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Jenny Scott & Michael G Rowan Department of Pharmacy & Pharmacology

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1 Harm reduction advice based on the analysis of crack cocaine seizures from Swindon, UK
Jenny Scott & Michael G Rowan Department of Pharmacy & Pharmacology University of Bath, Claverton Down, Bath, UK BA2 7AY

2 Background Here we present the results of a collaboration between a drugs service, police and academics. We offer one way to expand harm reduction advice for crack cocaine users. Anecdotal reports from Drugs & Homeless Initiative (DHI) clients suggested that crack obtained in Swindon (UK) was of low quality. DHI workers felt this provided a harm reduction opportunity and approached the University of Bath for advice. It is hypothesised that providing information about the content and quality of local crack could influence informed choices made by users. The police felt additional forensic information, not routinely collected, could be helpful in expanding police intelligence. This study analysed crack samples provided by the police seized in the Swindon area. Major adulterants and cocaine content was determined. This was translated into key messages for users.

3 Method Crack cocaine samples were donated to the University by Wiltshire police once cases were closed. 2 x 100mg was from removed from each ‘rock’ for analysis. 100mg was shaken with 2ml dichloromethane and 2ml water and the two layers separated. If the water layer pH was basic (>pH7) this indicated the crack was made by the bicarbonate method. This was titrated with acid to determine residual carbonate. If the water layer pH was acidic (<pH7) this indicated the crack could have been made by the ammonia method. High Performance Liquid Chromatography (HPLC) was used to determine any salt form cocaine in the water layer.

4 Method The dichloromethane layer was assayed by HPLC to measure cocaine base content. Analysis methods were based on the British Pharmacopoeia assay for cocaine. Analysis was performed in duplicate and average results calculated. Nuclear Magnetic Resonance (NMR) was performed on a separate portion (~ 60mg) of the ‘rock’ to identify major contaminants.

5 Results 50 samples have been analysed so far.

6 Content Not all contained crack cocaine.
40 (80%) samples contained cocaine. 38 (76%) of the samples contained cocaine in base form, four of which also contained salt form; one (2%) contained 56.1% salt and 2.1% base 2 (4%) contained salt form cocaine only 10 (20%) samples were not found to contain any cocaine. Of these: 5 appeared to be heroin one probably citric acid 4 unknown

7 Weight of ‘crack’ seizures
Fig 1: weight distribution of crack seizures (g) mean = 0.58g (SD =0.91) mode= 0.23g min = 0.05g max = 4.10g (n=37, 3 missing) ‘rock’ mean = 0.23g (SD=0.08) ‘powders and lumps’

8 Probable crack manufacturing method
30 by Bicarbonate method 8 possibly by Ammonia method 2 pH not determined

9 Cocaine base content of rocks
Cocaine base content ranged from 2.1 to 88.0%. Fig 2: cocaine base content of crack rocks mean = 39.4% (SD =17.6) mode =39.6% min =2.1% max =88.0%

10 Contaminants Contaminants could be identified in 36 (90%) of the cocaine samples. phenacetin n =29 (81%) benzocaine n = 3 (8%) glucose n= 3 (8%) mannitol n= 1 (3%) Phenacetin : cocaine ratio ranged from 0.3:1 to 2.5:1 w/w 1 (3%) sample contained cocaine with no identified organic contaminants, 3 were not examined by NMR. mean =1.68 (SD=0.76) mode =1.23 max=4.11 min =0.0

11 cocaine: phenacetin 2:1 cocaine: phenacetin 1:2
Fig 3. NMR spectra showing cocaine and phenacetin standards and a sample with a high cocaine: phenacetin ratio Fig 4: NMR spectra showing cocaine and phenacetin standards and a sample with a low cocaine: phenacetin ratio

13 Harm reduction messages
90% of ‘crack’ samples analysed had contaminants in them The most common, phenacetin, is potentially harmful to kidneys There was huge variation in cocaine ‘purity’ with the most common being ~ 40%; so 3/5th of a Swindon ‘crack rock’ isn’t cocaine. Maybe we could use NMR spectra for visual illustration to users?

14 Summary The analysis concurred with the user reports that Swindon crack was of low quality; 20% of cases contained no cocaine. The average content was 39%, UK average content (2003/4) is reported to be higher (69%) [Schifano & Corkery, 2008]. Although…. Most samples were contaminated with phenacetin, present in the ratio 0.3:1 to 2.5:1 with respect to cocaine. Phenacetin is not licensed as a medicine in the EU and associated with kidney damage in crack users [Brunt et al, 2009].

15 Conclusions Although the data is not novel in the analytical sense – the contaminants are previously reported in the literature and purity data is available, the local context of the analysis is novel and provides users with data from their own streets. Local harm reduction messages can inform users that analysed crack was of low cocaine content and may present a risk to kidney health. The main limit of this work is the time delay necessary between seizure and analysis. Seizures were made 2-3 years before analysis. The data is therefore retrospective, samples were collected over a one year period.

16 Acknowledgements Wiltshire police
Drugs and Homeless Initiative, Swindon, especially Mick Webb My colleague Dr Mike Rowan, who cannot be here today, provides the chemistry expertise and is credited with undertaking the analysis work Dr Tim Woodman, NMR Spectroscopist, University of Bath

Download ppt "Jenny Scott & Michael G Rowan Department of Pharmacy & Pharmacology"
 Acidity and Alkalinity Definitions Alkalinity Measurement Task Acidity Measurement Importance. Red Cabbage PH Indicator Rachel Jarrett Matt Marthaler.  In this experiment, we will determine the concentration of carbonate and bicarbonate species in different sodas using. Neutralization Reactions Reaction that occurs between an acid and a base. Example: HCl + NaOH  NaCl + H 2 O & Water Products always include: A “Salt” Slide 1 of 45  Worked Examples Follow:. Slide 2 of 45. Lecture 24 10/31/05. Finding endpoint with pH electrode. Acids, Bases, pH and Buffers. Acids An acid dissolves in water to donate H + In water the H+ reacts with water to make the hydronium ion H + + H 2 O  Titrations. Titration Method for determining the concentration of an unknown solution A measured volume of a solution of known concentration (titrant) Amal Almuhanna In most natural waters bicarbonates and sometimes carbonates are present in appreciable amounts. Their salts get hydrolyzed in solution. SCH3U Solutions and Solubility Lecture 4 Thioacidolysis and Derivatization Followed by Reductive Cleavage. Chemical synthesis Lesson 5. Learning objective: To understand how the purity of a sample can be determined by performing a titration. Must: Give examples. What color of bell peppers has the most vitamin C? Ronnie Groller and EJ Habina. Intro The purpose of our experiment was to test water from several different sources for hardness, ions, and pH. Our samples. Alkalinity Bicarbonate-carbonate. Alkalinity is… …the measure of the ability of a water to neutralize an acid. © University of South Carolina Board of Trustees Chapt. 16 More Acids and Bases Sec. 2 Titration: Strong Acid + Strong Base. Chemical Weathering. I. Introduction Chemical Weathering I. Introduction II. Process of Decomposition A. Overview: Decomposition alters minerals into. Alkalinity, Hardness, & Lime/Soda Ash Softening

 Titration. Weak Acid/Strong Base Titration Curve pH starts higher Rapid rise and then levels into a zone where pH doesn’t change much (buffering zone)