document: Extended depth-of-focus imaging of chlorophyll fluorescence from intact leaves Imaging dynamic changes in chlorophyll a fluorescence provides a valuable means with which to examine localised changes in photosynthetic function . Microscope-based systems provide excellent spatial resolution which allows the response of individual cells to be measured . However , such systems have a restricted depth of focus and , as leaves are inherently uneven , only a small proportion of each image at any given focal plane is in focus . In this report we describe the development of algorithms , specifically adapted for imaging chlorophyll fluorescence and photosynthetic function in living plant cells , which allow extended-focus images to be reconstructed from images taken in different focal planes . We describe how these procedures can be used to reconstruct images of chlorophyll fluorescence and calculated photosynthetic parameters , as well as producing a map of leaf topology . The robustness of this procedure is demonstrated using leaves from a number of different plant species
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