document: Rapid microwell polymerase chain reaction with subsequent ultrathin-layer gel electrophoresis of DNA Large-scale genotyping , mapping and expression profiling require affordable , fully automated high-throughput devices enabling rapid , high-performance analysis using minute quantities of reagents . In this paper , we describe a new combination of microwell polymerase chain reaction -LRB- PCR -RRB- based DNA amplification technique with automated ultrathin-layer gel electrophoresis analysis of the resulting products . This technique decreases the reagent consumption -LRB- total reaction volume 0.75-1 mu L -RRB- , the time requirement of the PCR -LRB- 15-20 min -RRB- and subsequent ultrathin-layer gel electrophoresis based fragment analysis -LRB- 5 min -RRB- by automating the current manual procedure and reducing the human intervention using sample loading robots and computerized real time data analysis . Small aliquots -LRB- 0.2 mu L -RRB- of the submicroliter size PCR reaction were transferred onto loading membranes and analyzed by ultrathin-layer gel electrophoresis which is a novel , high-performance and automated microseparation technique . This system employs integrated scanning laser-induced fluorescence-avalanche photodiode detection and combines the advantages of conventional slab and capillary gel electrophoresis . Visualization of the DNA fragments was accomplished by `` in migratio '' complexation with ethidium bromide during the electrophoresis process also enabling real time imaging and data analysis
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