Plasmids in environmental microbes: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology
https://journals.asm.org/doi/epub/10.1128/mbio.03191-22 Most studies of plasmids focus on traits like antibiotic resistance, which account for ~1% of plasmid genes. This study looked broadly at plasmids to better understand their composition and distribution as a function of environment. 

https://www.sciencedirect.com/science/article/pii/S1369527422000364 The studies described here investigate how microbial communities alter conjugative plasmid transfer and persistence. 

Pseudomonas https://www.addgene.org/search/catalog/plasmids/?q=pseudomonas This commercial site contains a large list of plasmids suitable for research with Pseudomonas . It includes plasmid maps and other useful information. 

https://royalsocietypublishing.org/doi/10.1098/rstb.2020.0461 This opinion piece discusses the role of plasmids in vertical and horizontal transfer and how plasmids might modulate their hosts for their own benefit in some instances. 

https://blog.addgene.org/plasmids-101-positive-and-negative-selection-for-plasmid-cloning The blog post here discusses the basics of using plasmids for negative and positive selection purposes. 

https://www.nature.com/articles/s41467-019-10600-7 This paper describes how plasmids might persist over evolutionary time scales without having selectable markers associated with the plasmid. 

https://pubs.acs.org/doi/10.1021/acssynbio.0c00466 This report documents that Escherichia coli Nissle 1917 natively contains two plasmids, the plasmids are maintained without selection, and they can be engineered for molecular engineering. 

https://phys.org/news/2022-12-reassemble-plasmid-genes-diverse-environmental.html This news article describes research in which an environmentally‐derived plasmid was engineered to be highly promiscuous. It could transfer between an Escherichia coli, Pseudomonas putida, Bacillus subtilis and Saccharomyces cerevisiae . 

https://journals.asm.org/doi/epub/10.1128/microbiolspec.plas-0033-2014 Here is reviewed plasmids and molecular parts of vectors than can be used for engineering purposes. 

https://www.addgene.org/vector-database/query/ The Vector database currently has 4,399 entries with information on vector backbones. It is compiled by Addgene but the resources are from third party sources and are not their commercial plasmids. There is a link to search Addgene plasmids for those interested. 

http://www.geneinfinity.org/sp/sp_plasmidsandvectors.html This page provides links to collections of plasmid and vector resources. 

https://www.snapgene.com/plasmids This site contains plasmid annotations and sequences with a focus on cloning vectors from different commercial sources. 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581055/pdf/961.pdf As more microbes are subjected to genome sequencing, and particularly with metagenome sequencing, it is becoming more important to separately identify plasmid DNA from chromosomal DNA. 

https://www.ebi.ac.uk/genomes/plasmid.html While this page is not being updated, it still has a useful collection of microbial plasmid sequences. 

