A Study on Postmortem Wound Dating by Gross and Histopathological Examination of Abrasions
This is a study on postmortem wound dating by gross and histopathological examination of abrasions. 

To determine the age of abrasions by gross and histopathological techniques. To correlate gross and histopathological changes in relation to time of injury. 

Source of Data This study was conducted in the Department of Forensic Medicine, M.S. Ramaiah Medical College, from October 2011 to March 2013, for a period of 18 months. Method of Collection of Data All fatal cases having well-demarcated wounds and known time of injury were included among the cases subjected to medicolegal autopsy. A total of 101 samples after fulfilling the inclusion criteria were taken up in the study. A standard proforma was used to collect information regarding time of injury, time of death, and associated comorbidities. Consent for tissue section was obtained after detailed interviews with investigating officer, relatives, friends, or whoever witnessed the incident and hospital records of the deceased. Standard autopsy protocol was followed; relevant details of the injuries such as site, size, and color were taken into consideration, and the age of injury was assessed depending on appearance. Then, injuries were grouped under 7 different time intervals—0 to 4, 4 to 12, 12 to 24, and 24 to 72 hours; 4 to 6 and 7 to 14 days; and more than 2 weeks—based on the survival period after the infliction of injury. The representative areas of injury with adjacent normal skin were sampled along with control sample from a nearer tissue of injury that was taken and preserved in 10% formalin for a day, then samples were processed, and blocks were created using paraffin. The process of embedding in paraffin blocks allows the sectioning of tissues into very thin (2–7 μm) sections using a microtome. The slices are thinner than the average cell and are layered on a glass slide for staining by hematoxylin and eosin (often abbreviated as H&E). Then, the slide is viewed under a microscope. Von Gieson staining was performed whenever necessary to confirm the presence of collagen tissue. 

This study was conducted in the Department of Forensic Medicine, M.S. Ramaiah Medical College, from October 2011 to March 2013, for a period of 18 months. 

All fatal cases having well-demarcated wounds and known time of injury were included among the cases subjected to medicolegal autopsy. A total of 101 samples after fulfilling the inclusion criteria were taken up in the study. A standard proforma was used to collect information regarding time of injury, time of death, and associated comorbidities. Consent for tissue section was obtained after detailed interviews with investigating officer, relatives, friends, or whoever witnessed the incident and hospital records of the deceased. Standard autopsy protocol was followed; relevant details of the injuries such as site, size, and color were taken into consideration, and the age of injury was assessed depending on appearance. Then, injuries were grouped under 7 different time intervals—0 to 4, 4 to 12, 12 to 24, and 24 to 72 hours; 4 to 6 and 7 to 14 days; and more than 2 weeks—based on the survival period after the infliction of injury. The representative areas of injury with adjacent normal skin were sampled along with control sample from a nearer tissue of injury that was taken and preserved in 10% formalin for a day, then samples were processed, and blocks were created using paraffin. The process of embedding in paraffin blocks allows the sectioning of tissues into very thin (2–7 μm) sections using a microtome. The slices are thinner than the average cell and are layered on a glass slide for staining by hematoxylin and eosin (often abbreviated as H&E). Then, the slide is viewed under a microscope. Von Gieson staining was performed whenever necessary to confirm the presence of collagen tissue. 

Inclusion criteria are cases where the time of infliction of injury was known. Exclusion criteria are bodies in the state of decomposition. Ethical clearance was obtained (ref. no. STD-1/EC/0416/2011). 

A total of 101 samples were taken up for the study. In the study, of 101cases, 89 cases (88.1%) were men because they are more into outdoor activities such as driving vehicles, labor work, and so forth, and the remaining 12 cases (11.9%) were women. Most were those in the age group of 21 to 40 years (51 cases), followed by 41 to 60 years (34 cases) as shown in Table . The reason was that they form the work group and are more prone to injuries due to road traffic accidents, fall, assaults, and so forth. A similar observation was made in a study conducted by Sharma et al on 60 corpses, where the maximum number of cases, that is, 24 cases (40%), belong to the age group of 21 to 30 years, followed by the age groups of 31 to 40, 41 to 50, and 51 to 60 years (13.34% of cases in each group). The minimum number of cases was observed in the age groups of younger than 10, 61 to 70 years, and older than 70 (3.34% of cases in each group). It was observed in another study that, of 35 total cases in the age group of 1 to 80 years, maximum cases belonged to the age group of 31 to 40 years, comprising 25 men and 10 women. Of the 101 total cases, 89 cases (75.4%) were due to road traffic accident, 10 cases (8.5%) were due to fall from height, 1 case (0.8%) was due to railway injury, and the remaining 1 case (0.8%) was due to assault. Among the 101 cases, 29 cases (24.6%) were 0 to 4 hours old, 11 cases (9.3%) were of the duration of 4 to 12 hours, 10 cases (8.5%) were 12 to 24 hours old, 18 cases (15.3%) were 24 to 72 hours old, 8 cases (6.8%) were 4 to 6 days old, 16 cases (13.6%) were 7 to 14 days old, and the remaining 9 cases (7.6%) were more than 2 weeks old (Fig. ). Gross Changes One hundred one abrasions were studied (Table ), among which 33 (32.7%) showed bright red, 22 (21.8%) showed reddish scab, 16 (15.8%) showed brownish scab, 3 (3.0%) showed dark brown scab, 10 (9.9%) showed black scab, and in 13 injuries (12.9%), the scab had fallen off at margins. In 4 injuries (4.0%), the scab had fallen off completely. Bright red color was observed in 33 abrasions; 29 (87.9%) of these injuries were 0 to 4 hours old. The remaining 4 injuries were 4 to 12 hours old, more so between 4 and 5 hours, and were located in the less vascular areas of the extremities. No comorbidities were associated in any of these cases. Bright redness was observed earliest at 10 minutes and latest up to 5 hours. This was in agreement with the study conducted by Sharma et al where they found redness from 10 minutes up to 7 hours. In contrary, it was observed in another study that most cases on the first day were dark red instead of bright red in color. Reddish scab was noted in 22 injuries, of which 8 injuries (36.4%) were 12 to 24 hours old. The remaining 14 were distributed equally (31.8% each) in the durations of 4 to 12 and 24 to 72 hours. Reddish scab was observed earliest at 6 hours and latest up to 68 hours. A similar observation was made by Sharma et al in their study. An author also quotes that reddish scab forms by 8 to 24 hours. Brownish scab was noted in 16 injuries, of which 10 injuries (62.5%) were 24 to 72 hours old; the remaining 2 were 12 to 24 hours old, and 4 were 4 to 6 days old. The earliest brownish scab was noted at 18 hours in 2 injuries, where both injuries were small in size and the deceased was young who did not have comorbidities. The latest appearance of the brownish scab was at 132 hours (5.5 days). A similar observation was made by Sharma et al, and an author also quotes that the scab looks brownish by the second and third days. This was in contrast with the study conducted by Kumar et al wherein they observed that, on the third day, most injuries were dark red in color instead of brownish. The dark brown scab was noted in 3 injuries, of which 2 injuries (66.7%) were 4 to 6 days old and the remaining was 24 to 72 hours old. The earliest dark brown scab was noted at 44 hours, where the injury was situated over the face (more vascular area). The other 2 injuries were at 97 and 144 hours; however, their sizes (2 × 1 and 5 × 3 cm) were small, and the deceased did not have any comorbidity. In a similar study, Sharma et al found a hard brown scab 27 hours onward, and an author also quotes that, by the fourth and fifth days, the scab looks dark brown. However, this was in disagreement with another study where they observed a dark red scab instead of a dark brownish scab on the fifth day. The black scab was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old, 1 injury was 4 to 6 days old, and 2 were observed at more than 2 weeks. The black scab was seen only after 5 days, was seen at 21 days in a deceased who had no comorbidities, and was situated in the leg (less vascular area). In another case, a black scab was noted at 35 days in an elderly man, who was poorly nourished and comatose, died of secondary infections consequent to sustained head injury, and had a history of diabetes and hypertension. A scab fallen at the margin was seen in 13 injuries, of which 8 injuries (61.5%) were of 7 to 14 days and the remaining 4 were more than 2 weeks old. The fall of scab was noted only after 6 days and was seen up to 15 days in a healthy individual where injury was located in the lower extremity (knee). In the remaining 3 cases, there was significant delay of fall of scab observed up to 45 days. All 3 of them were experiencing septicemia. In 4 injuries, the scabs had fallen off completely, of which 3 injuries (75.0%) were more than 2 weeks old, and in the remaining injury, the scab had fallen off at 11 days itself. In a healthy individual, it took up to 17 days for the scab to fall off completely. However, in a comatose patient, who had comorbidities such as diabetes and septicemia, the fall of scab (complete) was observed on the 27th day. In a similar study by Sharma et al, they found that the black scab started separating 7 days onward, and the authors quote that, by the sixth day, it turned blackish and started falling off from the margins. A big scab takes a few more days to fall off. However, this was in disagreement with the study where they observed a dark brown scab instead of a black scab on the ninth day. The Scoring for Microscopic Changes as Given in the Following Order 0. Hemorrhage and/or congestion of vessels 1. Edema formation 2. Margination of polymorph cells 3. Early infiltration of polymorph cells 4. Predominant polymorph infiltration with poorly differentiated mononuclear cells 5. Predominant mononuclear cell infiltration 6. Appearance of fibroblast 7. Granulation tissue with rich leukocyte infiltration and much fibroblasts 8. Collagen tissue deposition 9. Cellular reaction subsides; fibroblast are more active with increased collagen formation (regression phase of injury) 10. Organized pustule formation in the presence of infection Microscopic Changes Table shows among 30 abrasions, which showed only hemorrhage and/or congestion of vessels, 23 (76.7%) were 0 to 4 hours old, 6 were 4 to 12 hours old, and one was 12 to 24 hours old. The presence of congestion of vessels and/or hemorrhage was observed from 10 minutes and lasts up to 6 hours, except in 2 where it was observed at 11 and 14 hours. The earliest formation of edema was noted at 15 minutes. Margination of polymorphs was observed during 0 to 4 hours in 5 (62.5%) of 8 injuries. This was seen earliest at 30 minutes and lasted up to 5 hours. Early polymorphs infiltration was observed in 3 injuries, of which 2 injuries (66.7%) were 4 to 12 hours old. It was seen earliest at 6 hours. A similar observation was made in a study by Sharma et al where early polymorph infiltration was found at 7 hours, and another author also quotes that the margination of polymorph occurs by 4 hours. An author quotes that the polymorph infiltration in a perivascular fashion was seen from 2 to 6 hours. On the contrary, in another study, the author comments that the earliest appearance of polymorphs was observed at 20 to 30 minutes after an injury. Prominent polymorph infiltration was observed in 15 injuries, of which 8 (53.3%) were 12 to 24 hours old and the remaining 7 (46.7%) were 24 to 72 hours old. It was noted earliest at 12 hours, and progressive increase was noted in the 24- to 72-hour-old injuries. A similar observation was made by Ross and Benditt, where, in less than 12 hours, the wound was rich in polymorph nuclear leukocytes, and the numbers of those cells increased for the first 24 hours to a maximum. An author also comments that infiltration progressively increases from 12 to 18 hours. A similar observation was made by Sharma et al in their study. Mononuclear cell infiltration was first noted at 24 hours, and increased infiltration was observed in 6 injuries during 24 to 72 hours. It was noted that predominant mononuclear infiltration was between 35 and 50 hours in 5 injuries. The earliest regenerative change of epithelium was noted at 2 days. The active fibroblasts were evident only after 71 hours. In a similar study by Sharma et al, early mononuclear infiltration was observed at 13 hours itself, whereas we noticed that in our study at 24 hours. Similar observations were made by an author where the earliest mononuclear infiltration was noted at 20 hours. Another author quotes that the regeneration of epithelium starts as early as 30 hours and is clearly visible by 72 hours in most cases. The granulation tissue deposition was noted in 13 injuries, of which 5 (38.5%) were 4 to 6 days old, 4 were 24 to 72 hours old, and the remaining 3 injuries were 7 to 14 days old. The early granulation tissue (neovascularization) was noted at 72 hours, and an increase in the deposition of granulation tissue was observed routinely by 4 to 6 days. This observation is in agreement with an author who found that the granulation tissue formation is seen by 5 to 8 days, and another author also quotes the similar finding. The collagen tissue was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old. The earliest collagen formation was seen at 96 hours and more commonly in 7- to 14-day-old injuries. This was in agreement with a study where collagen formation started after the third day. A similar observation was made in another study by Ross and Benditt, and an author quotes that, at 3 to 6 days, collagen formation begins and later increases in density. The regression phase was noted in 10 injuries, of which 6 (60.0%) were more than 2 weeks old and the remaining injuries were 7 to 14 days old. The earliest regression phase was noted at 9 days and was more common in injuries of more than 2 weeks old. In a similar study by Ross and Benditt, at 14 days, the morphologic features were parallel to those of the earlier periods. However, another author quotes that the regression phase will start by the 12th day. In 2 injuries, the formation of organized pustule rich in polymorphs was noted during the period of 7 to 14 days and more than 2 weeks old. Correlation of Gross Changes With Microscopic Changes Table shows among 33 abrasions of bright red color, 24 (72.7%) had hemorrhage and/or congestion of vessels, 1 was associated with edema, and the remaining showed margination of polymorphs. A reddish scab was noted in 22 abrasions. Among them, 9 (40.9%) had predominant polymorphs infiltration, 6 had only hemorrhage and/or congestion of vessels, 3 had margination of polymorph cells, 3 had significant mononuclear cell infiltration, and 2 showed granulation tissue deposition. A brownish scab was noted in 16 abrasions, among which 6 (37.5%) had predominant polymorph cell infiltration, 4 had granulation tissue, 3 had mononuclear cell infiltration, and 2 injuries had active fibroblasts. A dark brown scab was noted in 3 abrasions, among which 2 (66.7%) showed dense granulation tissue. A black scab was noted in 10 abrasions; among them, 4 injuries (40%) had collagen tissue, 2 injuries showed granulation tissue, 2 injuries were in the regression phase, and in 1 case, there was organized pustule. A scab had fallen off at margin in 13 abrasions; most of them showed collagen tissue deposition or were in the regression phase (38.5% each), and in 1 injury, there was organized pustule. Scabs had fallen off completely in 4 injuries, where most of these injuries were in the regression phase (75%). By comparing gross changes with microscopic changes, among the injuries of 4 to 6 days in duration, 2 (25%) of 8 abrasions showed a dark brown scab, whereas by histological study, 5 (62.5%) of them showed granulation tissue, which confirmed the age of injury to be more than 4 days. Among the injuries of more than 2 weeks old, 3 (33.3%) of 9 abrasions showed a scab that has fallen off completely, whereas by histological study, 66.7% of the injuries showed dense collagen tissue with decreased cellular reaction, which confirms the injury as more than 2 weeks old. 

One hundred one abrasions were studied (Table ), among which 33 (32.7%) showed bright red, 22 (21.8%) showed reddish scab, 16 (15.8%) showed brownish scab, 3 (3.0%) showed dark brown scab, 10 (9.9%) showed black scab, and in 13 injuries (12.9%), the scab had fallen off at margins. In 4 injuries (4.0%), the scab had fallen off completely. Bright red color was observed in 33 abrasions; 29 (87.9%) of these injuries were 0 to 4 hours old. The remaining 4 injuries were 4 to 12 hours old, more so between 4 and 5 hours, and were located in the less vascular areas of the extremities. No comorbidities were associated in any of these cases. Bright redness was observed earliest at 10 minutes and latest up to 5 hours. This was in agreement with the study conducted by Sharma et al where they found redness from 10 minutes up to 7 hours. In contrary, it was observed in another study that most cases on the first day were dark red instead of bright red in color. Reddish scab was noted in 22 injuries, of which 8 injuries (36.4%) were 12 to 24 hours old. The remaining 14 were distributed equally (31.8% each) in the durations of 4 to 12 and 24 to 72 hours. Reddish scab was observed earliest at 6 hours and latest up to 68 hours. A similar observation was made by Sharma et al in their study. An author also quotes that reddish scab forms by 8 to 24 hours. Brownish scab was noted in 16 injuries, of which 10 injuries (62.5%) were 24 to 72 hours old; the remaining 2 were 12 to 24 hours old, and 4 were 4 to 6 days old. The earliest brownish scab was noted at 18 hours in 2 injuries, where both injuries were small in size and the deceased was young who did not have comorbidities. The latest appearance of the brownish scab was at 132 hours (5.5 days). A similar observation was made by Sharma et al, and an author also quotes that the scab looks brownish by the second and third days. This was in contrast with the study conducted by Kumar et al wherein they observed that, on the third day, most injuries were dark red in color instead of brownish. The dark brown scab was noted in 3 injuries, of which 2 injuries (66.7%) were 4 to 6 days old and the remaining was 24 to 72 hours old. The earliest dark brown scab was noted at 44 hours, where the injury was situated over the face (more vascular area). The other 2 injuries were at 97 and 144 hours; however, their sizes (2 × 1 and 5 × 3 cm) were small, and the deceased did not have any comorbidity. In a similar study, Sharma et al found a hard brown scab 27 hours onward, and an author also quotes that, by the fourth and fifth days, the scab looks dark brown. However, this was in disagreement with another study where they observed a dark red scab instead of a dark brownish scab on the fifth day. The black scab was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old, 1 injury was 4 to 6 days old, and 2 were observed at more than 2 weeks. The black scab was seen only after 5 days, was seen at 21 days in a deceased who had no comorbidities, and was situated in the leg (less vascular area). In another case, a black scab was noted at 35 days in an elderly man, who was poorly nourished and comatose, died of secondary infections consequent to sustained head injury, and had a history of diabetes and hypertension. A scab fallen at the margin was seen in 13 injuries, of which 8 injuries (61.5%) were of 7 to 14 days and the remaining 4 were more than 2 weeks old. The fall of scab was noted only after 6 days and was seen up to 15 days in a healthy individual where injury was located in the lower extremity (knee). In the remaining 3 cases, there was significant delay of fall of scab observed up to 45 days. All 3 of them were experiencing septicemia. In 4 injuries, the scabs had fallen off completely, of which 3 injuries (75.0%) were more than 2 weeks old, and in the remaining injury, the scab had fallen off at 11 days itself. In a healthy individual, it took up to 17 days for the scab to fall off completely. However, in a comatose patient, who had comorbidities such as diabetes and septicemia, the fall of scab (complete) was observed on the 27th day. In a similar study by Sharma et al, they found that the black scab started separating 7 days onward, and the authors quote that, by the sixth day, it turned blackish and started falling off from the margins. A big scab takes a few more days to fall off. However, this was in disagreement with the study where they observed a dark brown scab instead of a black scab on the ninth day. The Scoring for Microscopic Changes as Given in the Following Order 0. Hemorrhage and/or congestion of vessels 1. Edema formation 2. Margination of polymorph cells 3. Early infiltration of polymorph cells 4. Predominant polymorph infiltration with poorly differentiated mononuclear cells 5. Predominant mononuclear cell infiltration 6. Appearance of fibroblast 7. Granulation tissue with rich leukocyte infiltration and much fibroblasts 8. Collagen tissue deposition 9. Cellular reaction subsides; fibroblast are more active with increased collagen formation (regression phase of injury) 10. Organized pustule formation in the presence of infection Microscopic Changes Table shows among 30 abrasions, which showed only hemorrhage and/or congestion of vessels, 23 (76.7%) were 0 to 4 hours old, 6 were 4 to 12 hours old, and one was 12 to 24 hours old. The presence of congestion of vessels and/or hemorrhage was observed from 10 minutes and lasts up to 6 hours, except in 2 where it was observed at 11 and 14 hours. The earliest formation of edema was noted at 15 minutes. Margination of polymorphs was observed during 0 to 4 hours in 5 (62.5%) of 8 injuries. This was seen earliest at 30 minutes and lasted up to 5 hours. Early polymorphs infiltration was observed in 3 injuries, of which 2 injuries (66.7%) were 4 to 12 hours old. It was seen earliest at 6 hours. A similar observation was made in a study by Sharma et al where early polymorph infiltration was found at 7 hours, and another author also quotes that the margination of polymorph occurs by 4 hours. An author quotes that the polymorph infiltration in a perivascular fashion was seen from 2 to 6 hours. On the contrary, in another study, the author comments that the earliest appearance of polymorphs was observed at 20 to 30 minutes after an injury. Prominent polymorph infiltration was observed in 15 injuries, of which 8 (53.3%) were 12 to 24 hours old and the remaining 7 (46.7%) were 24 to 72 hours old. It was noted earliest at 12 hours, and progressive increase was noted in the 24- to 72-hour-old injuries. A similar observation was made by Ross and Benditt, where, in less than 12 hours, the wound was rich in polymorph nuclear leukocytes, and the numbers of those cells increased for the first 24 hours to a maximum. An author also comments that infiltration progressively increases from 12 to 18 hours. A similar observation was made by Sharma et al in their study. Mononuclear cell infiltration was first noted at 24 hours, and increased infiltration was observed in 6 injuries during 24 to 72 hours. It was noted that predominant mononuclear infiltration was between 35 and 50 hours in 5 injuries. The earliest regenerative change of epithelium was noted at 2 days. The active fibroblasts were evident only after 71 hours. In a similar study by Sharma et al, early mononuclear infiltration was observed at 13 hours itself, whereas we noticed that in our study at 24 hours. Similar observations were made by an author where the earliest mononuclear infiltration was noted at 20 hours. Another author quotes that the regeneration of epithelium starts as early as 30 hours and is clearly visible by 72 hours in most cases. The granulation tissue deposition was noted in 13 injuries, of which 5 (38.5%) were 4 to 6 days old, 4 were 24 to 72 hours old, and the remaining 3 injuries were 7 to 14 days old. The early granulation tissue (neovascularization) was noted at 72 hours, and an increase in the deposition of granulation tissue was observed routinely by 4 to 6 days. This observation is in agreement with an author who found that the granulation tissue formation is seen by 5 to 8 days, and another author also quotes the similar finding. The collagen tissue was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old. The earliest collagen formation was seen at 96 hours and more commonly in 7- to 14-day-old injuries. This was in agreement with a study where collagen formation started after the third day. A similar observation was made in another study by Ross and Benditt, and an author quotes that, at 3 to 6 days, collagen formation begins and later increases in density. The regression phase was noted in 10 injuries, of which 6 (60.0%) were more than 2 weeks old and the remaining injuries were 7 to 14 days old. The earliest regression phase was noted at 9 days and was more common in injuries of more than 2 weeks old. In a similar study by Ross and Benditt, at 14 days, the morphologic features were parallel to those of the earlier periods. However, another author quotes that the regression phase will start by the 12th day. In 2 injuries, the formation of organized pustule rich in polymorphs was noted during the period of 7 to 14 days and more than 2 weeks old. Correlation of Gross Changes With Microscopic Changes Table shows among 33 abrasions of bright red color, 24 (72.7%) had hemorrhage and/or congestion of vessels, 1 was associated with edema, and the remaining showed margination of polymorphs. A reddish scab was noted in 22 abrasions. Among them, 9 (40.9%) had predominant polymorphs infiltration, 6 had only hemorrhage and/or congestion of vessels, 3 had margination of polymorph cells, 3 had significant mononuclear cell infiltration, and 2 showed granulation tissue deposition. A brownish scab was noted in 16 abrasions, among which 6 (37.5%) had predominant polymorph cell infiltration, 4 had granulation tissue, 3 had mononuclear cell infiltration, and 2 injuries had active fibroblasts. A dark brown scab was noted in 3 abrasions, among which 2 (66.7%) showed dense granulation tissue. A black scab was noted in 10 abrasions; among them, 4 injuries (40%) had collagen tissue, 2 injuries showed granulation tissue, 2 injuries were in the regression phase, and in 1 case, there was organized pustule. A scab had fallen off at margin in 13 abrasions; most of them showed collagen tissue deposition or were in the regression phase (38.5% each), and in 1 injury, there was organized pustule. Scabs had fallen off completely in 4 injuries, where most of these injuries were in the regression phase (75%). By comparing gross changes with microscopic changes, among the injuries of 4 to 6 days in duration, 2 (25%) of 8 abrasions showed a dark brown scab, whereas by histological study, 5 (62.5%) of them showed granulation tissue, which confirmed the age of injury to be more than 4 days. Among the injuries of more than 2 weeks old, 3 (33.3%) of 9 abrasions showed a scab that has fallen off completely, whereas by histological study, 66.7% of the injuries showed dense collagen tissue with decreased cellular reaction, which confirms the injury as more than 2 weeks old. 

One hundred one abrasions were studied (Table ), among which 33 (32.7%) showed bright red, 22 (21.8%) showed reddish scab, 16 (15.8%) showed brownish scab, 3 (3.0%) showed dark brown scab, 10 (9.9%) showed black scab, and in 13 injuries (12.9%), the scab had fallen off at margins. In 4 injuries (4.0%), the scab had fallen off completely. Bright red color was observed in 33 abrasions; 29 (87.9%) of these injuries were 0 to 4 hours old. The remaining 4 injuries were 4 to 12 hours old, more so between 4 and 5 hours, and were located in the less vascular areas of the extremities. No comorbidities were associated in any of these cases. Bright redness was observed earliest at 10 minutes and latest up to 5 hours. This was in agreement with the study conducted by Sharma et al where they found redness from 10 minutes up to 7 hours. In contrary, it was observed in another study that most cases on the first day were dark red instead of bright red in color. Reddish scab was noted in 22 injuries, of which 8 injuries (36.4%) were 12 to 24 hours old. The remaining 14 were distributed equally (31.8% each) in the durations of 4 to 12 and 24 to 72 hours. Reddish scab was observed earliest at 6 hours and latest up to 68 hours. A similar observation was made by Sharma et al in their study. An author also quotes that reddish scab forms by 8 to 24 hours. Brownish scab was noted in 16 injuries, of which 10 injuries (62.5%) were 24 to 72 hours old; the remaining 2 were 12 to 24 hours old, and 4 were 4 to 6 days old. The earliest brownish scab was noted at 18 hours in 2 injuries, where both injuries were small in size and the deceased was young who did not have comorbidities. The latest appearance of the brownish scab was at 132 hours (5.5 days). A similar observation was made by Sharma et al, and an author also quotes that the scab looks brownish by the second and third days. This was in contrast with the study conducted by Kumar et al wherein they observed that, on the third day, most injuries were dark red in color instead of brownish. The dark brown scab was noted in 3 injuries, of which 2 injuries (66.7%) were 4 to 6 days old and the remaining was 24 to 72 hours old. The earliest dark brown scab was noted at 44 hours, where the injury was situated over the face (more vascular area). The other 2 injuries were at 97 and 144 hours; however, their sizes (2 × 1 and 5 × 3 cm) were small, and the deceased did not have any comorbidity. In a similar study, Sharma et al found a hard brown scab 27 hours onward, and an author also quotes that, by the fourth and fifth days, the scab looks dark brown. However, this was in disagreement with another study where they observed a dark red scab instead of a dark brownish scab on the fifth day. The black scab was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old, 1 injury was 4 to 6 days old, and 2 were observed at more than 2 weeks. The black scab was seen only after 5 days, was seen at 21 days in a deceased who had no comorbidities, and was situated in the leg (less vascular area). In another case, a black scab was noted at 35 days in an elderly man, who was poorly nourished and comatose, died of secondary infections consequent to sustained head injury, and had a history of diabetes and hypertension. A scab fallen at the margin was seen in 13 injuries, of which 8 injuries (61.5%) were of 7 to 14 days and the remaining 4 were more than 2 weeks old. The fall of scab was noted only after 6 days and was seen up to 15 days in a healthy individual where injury was located in the lower extremity (knee). In the remaining 3 cases, there was significant delay of fall of scab observed up to 45 days. All 3 of them were experiencing septicemia. In 4 injuries, the scabs had fallen off completely, of which 3 injuries (75.0%) were more than 2 weeks old, and in the remaining injury, the scab had fallen off at 11 days itself. In a healthy individual, it took up to 17 days for the scab to fall off completely. However, in a comatose patient, who had comorbidities such as diabetes and septicemia, the fall of scab (complete) was observed on the 27th day. In a similar study by Sharma et al, they found that the black scab started separating 7 days onward, and the authors quote that, by the sixth day, it turned blackish and started falling off from the margins. A big scab takes a few more days to fall off. However, this was in disagreement with the study where they observed a dark brown scab instead of a black scab on the ninth day. 

0. Hemorrhage and/or congestion of vessels 1. Edema formation 2. Margination of polymorph cells 3. Early infiltration of polymorph cells 4. Predominant polymorph infiltration with poorly differentiated mononuclear cells 5. Predominant mononuclear cell infiltration 6. Appearance of fibroblast 7. Granulation tissue with rich leukocyte infiltration and much fibroblasts 8. Collagen tissue deposition 9. Cellular reaction subsides; fibroblast are more active with increased collagen formation (regression phase of injury) 10. Organized pustule formation in the presence of infection 

Table shows among 30 abrasions, which showed only hemorrhage and/or congestion of vessels, 23 (76.7%) were 0 to 4 hours old, 6 were 4 to 12 hours old, and one was 12 to 24 hours old. The presence of congestion of vessels and/or hemorrhage was observed from 10 minutes and lasts up to 6 hours, except in 2 where it was observed at 11 and 14 hours. The earliest formation of edema was noted at 15 minutes. Margination of polymorphs was observed during 0 to 4 hours in 5 (62.5%) of 8 injuries. This was seen earliest at 30 minutes and lasted up to 5 hours. Early polymorphs infiltration was observed in 3 injuries, of which 2 injuries (66.7%) were 4 to 12 hours old. It was seen earliest at 6 hours. A similar observation was made in a study by Sharma et al where early polymorph infiltration was found at 7 hours, and another author also quotes that the margination of polymorph occurs by 4 hours. An author quotes that the polymorph infiltration in a perivascular fashion was seen from 2 to 6 hours. On the contrary, in another study, the author comments that the earliest appearance of polymorphs was observed at 20 to 30 minutes after an injury. Prominent polymorph infiltration was observed in 15 injuries, of which 8 (53.3%) were 12 to 24 hours old and the remaining 7 (46.7%) were 24 to 72 hours old. It was noted earliest at 12 hours, and progressive increase was noted in the 24- to 72-hour-old injuries. A similar observation was made by Ross and Benditt, where, in less than 12 hours, the wound was rich in polymorph nuclear leukocytes, and the numbers of those cells increased for the first 24 hours to a maximum. An author also comments that infiltration progressively increases from 12 to 18 hours. A similar observation was made by Sharma et al in their study. Mononuclear cell infiltration was first noted at 24 hours, and increased infiltration was observed in 6 injuries during 24 to 72 hours. It was noted that predominant mononuclear infiltration was between 35 and 50 hours in 5 injuries. The earliest regenerative change of epithelium was noted at 2 days. The active fibroblasts were evident only after 71 hours. In a similar study by Sharma et al, early mononuclear infiltration was observed at 13 hours itself, whereas we noticed that in our study at 24 hours. Similar observations were made by an author where the earliest mononuclear infiltration was noted at 20 hours. Another author quotes that the regeneration of epithelium starts as early as 30 hours and is clearly visible by 72 hours in most cases. The granulation tissue deposition was noted in 13 injuries, of which 5 (38.5%) were 4 to 6 days old, 4 were 24 to 72 hours old, and the remaining 3 injuries were 7 to 14 days old. The early granulation tissue (neovascularization) was noted at 72 hours, and an increase in the deposition of granulation tissue was observed routinely by 4 to 6 days. This observation is in agreement with an author who found that the granulation tissue formation is seen by 5 to 8 days, and another author also quotes the similar finding. The collagen tissue was noted in 10 injuries, of which 7 injuries (70.0%) were 7 to 14 days old. The earliest collagen formation was seen at 96 hours and more commonly in 7- to 14-day-old injuries. This was in agreement with a study where collagen formation started after the third day. A similar observation was made in another study by Ross and Benditt, and an author quotes that, at 3 to 6 days, collagen formation begins and later increases in density. The regression phase was noted in 10 injuries, of which 6 (60.0%) were more than 2 weeks old and the remaining injuries were 7 to 14 days old. The earliest regression phase was noted at 9 days and was more common in injuries of more than 2 weeks old. In a similar study by Ross and Benditt, at 14 days, the morphologic features were parallel to those of the earlier periods. However, another author quotes that the regression phase will start by the 12th day. In 2 injuries, the formation of organized pustule rich in polymorphs was noted during the period of 7 to 14 days and more than 2 weeks old. 

Table shows among 33 abrasions of bright red color, 24 (72.7%) had hemorrhage and/or congestion of vessels, 1 was associated with edema, and the remaining showed margination of polymorphs. A reddish scab was noted in 22 abrasions. Among them, 9 (40.9%) had predominant polymorphs infiltration, 6 had only hemorrhage and/or congestion of vessels, 3 had margination of polymorph cells, 3 had significant mononuclear cell infiltration, and 2 showed granulation tissue deposition. A brownish scab was noted in 16 abrasions, among which 6 (37.5%) had predominant polymorph cell infiltration, 4 had granulation tissue, 3 had mononuclear cell infiltration, and 2 injuries had active fibroblasts. A dark brown scab was noted in 3 abrasions, among which 2 (66.7%) showed dense granulation tissue. A black scab was noted in 10 abrasions; among them, 4 injuries (40%) had collagen tissue, 2 injuries showed granulation tissue, 2 injuries were in the regression phase, and in 1 case, there was organized pustule. A scab had fallen off at margin in 13 abrasions; most of them showed collagen tissue deposition or were in the regression phase (38.5% each), and in 1 injury, there was organized pustule. Scabs had fallen off completely in 4 injuries, where most of these injuries were in the regression phase (75%). By comparing gross changes with microscopic changes, among the injuries of 4 to 6 days in duration, 2 (25%) of 8 abrasions showed a dark brown scab, whereas by histological study, 5 (62.5%) of them showed granulation tissue, which confirmed the age of injury to be more than 4 days. Among the injuries of more than 2 weeks old, 3 (33.3%) of 9 abrasions showed a scab that has fallen off completely, whereas by histological study, 66.7% of the injuries showed dense collagen tissue with decreased cellular reaction, which confirms the injury as more than 2 weeks old. 

A postmortem study of wound dating by gross and histopathological examination of abrasions was carried out in the Department of Forensic Medicine, M.S. Ramaiah Medical College, from October 2011 to March 2013. A total of 101 samples (injuries) were taken up for the study. Most of them were in the age group of 21 to 40 years (50.5%), and men constituted more than three fourths (88.1%) of the cases. Furthermore, 75.4% of the cases were due to road traffic injuries, and the remaining were due to railway injuries, fall from height, and so forth. The results concluded are as follows: on gross examination, bright red color was seen in injuries less than 5 hours old. The reddish scab was seen up to 68 hours. A brown scab was seen after 18 hours. A dark brown scab was observed only after 44 hours. A black scab was observed in the injuries more than 5 days old. It took at least 6 days for the scab to fall from the margin and 11 days to fall off completely (Table ). On microscopic examination, hemorrhage and congestion of vessels were seen in injuries less than 14 hours old. Predominant polymorph cell infiltration was observed only after 6 hours. Mononuclear cell infiltration was observed in injuries more than 1 day old. The granulation tissue was seen after 72 hours. Collagen tissue formation was observed after 4 days (Table ). Among 4- to 6-day-old abrasions, the accuracy of wound dating was increased from 25% to 62.5%; similarly, among those more than 2 weeks old, the accuracy was increased from 33.3% to 66.7% by subjecting it for histological study. Role of Comorbid Conditions on Wound Healing In comatose patients with or without other comorbidities such as diabetes, hypertension, sepsis, malnutrition, and so forth, delay in the scab formation was observed. By microscopy, the infiltration of inflammatory cells (polymorphs and mononuclear cells) was observed in the infected wounds even up to 45 days. Hence, these factors are to be considered while giving opinion with regard to dating of the injury. To conclude, although gross features of the injuries provide a rough estimate regarding their age, histological examination will confirm or gives a more accurate timing of their occurrence, thus aiding in the administration of justice. 

In comatose patients with or without other comorbidities such as diabetes, hypertension, sepsis, malnutrition, and so forth, delay in the scab formation was observed. By microscopy, the infiltration of inflammatory cells (polymorphs and mononuclear cells) was observed in the infected wounds even up to 45 days. Hence, these factors are to be considered while giving opinion with regard to dating of the injury. To conclude, although gross features of the injuries provide a rough estimate regarding their age, histological examination will confirm or gives a more accurate timing of their occurrence, thus aiding in the administration of justice. 

In comatose patients with or without other comorbidities such as diabetes, hypertension, sepsis, malnutrition, and so forth, delay in the scab formation was observed. By microscopy, the infiltration of inflammatory cells (polymorphs and mononuclear cells) was observed in the infected wounds even up to 45 days. Hence, these factors are to be considered while giving opinion with regard to dating of the injury. To conclude, although gross features of the injuries provide a rough estimate regarding their age, histological examination will confirm or gives a more accurate timing of their occurrence, thus aiding in the administration of justice. 

In individuals with dark brown complexion, the early color changes are difficult to appreciate clearly, and more so, this type of naked eye observation is subjective. The degree of possible observer variation was not assessed. 

Further studies on enzyme histochemistry, biochemical assays, and fluorescence studies can be conducted and correlated with microscopic changes. This will help in removing subjectivity and bring more objectivity in the observation and interpretation. 

To the Autopsy Surgeon In an Indian scenario for dating of injuries, we rely on naked eye examination (subjective) of gross changes, which gives a rough estimate regarding the age; thus, subjecting the samples for histological examination would be more accurate. In cases with multiple injuries over different sites, the healing process may vary because of various factors, and thus, opining the age of the injury would be difficult and would not correlate with the time of infliction of the injury as alleged by the police or relatives; hence, the autopsy surgeon should exercise caution while opining the age. In histopathology slides during examination, the presence of artifacts in the form of sand, mud particles, glass pieces, and so forth is to be kept in mind. 

In an Indian scenario for dating of injuries, we rely on naked eye examination (subjective) of gross changes, which gives a rough estimate regarding the age; thus, subjecting the samples for histological examination would be more accurate. In cases with multiple injuries over different sites, the healing process may vary because of various factors, and thus, opining the age of the injury would be difficult and would not correlate with the time of infliction of the injury as alleged by the police or relatives; hence, the autopsy surgeon should exercise caution while opining the age. In histopathology slides during examination, the presence of artifacts in the form of sand, mud particles, glass pieces, and so forth is to be kept in mind. 

