<clinical_study>
  <!-- This xml conforms to an XML Schema at:
    https://clinicaltrials.gov/ct2/html/images/info/public.xsd -->
  <required_header>
    <download_date>ClinicalTrials.gov processed this data on September 20, 2023</download_date>
    <link_text>Link to the current ClinicalTrials.gov record.</link_text>
    <url>https://clinicaltrials.gov/ct2/show/NCT03807674</url>
  </required_header>
  <id_info>
    <org_study_id>BEC-LSMU(R)-01</org_study_id>
    <nct_id>NCT03807674</nct_id>
  </id_info>
  <brief_title>MMP-9 Expression in Permanent Mature Teeth With Symptomatic Pulpitis</brief_title>
  <official_title>A Prospective Clinical Pilot Study of MMP-9 Expression and Short-term Outcome of Coronal Pulpotomy in Mature Permanent Teeth With Symptomatic Pulpitis</official_title>
  <sponsors>
    <lead_sponsor>
      <agency>Eglė Gvazdaitytė</agency>
      <agency_class>Other</agency_class>
    </lead_sponsor>
  </sponsors>
  <source>Lithuanian University of Health Sciences</source>
  <oversight_info>
    <has_dmc>No</has_dmc>
    <is_fda_regulated_drug>No</is_fda_regulated_drug>
    <is_fda_regulated_device>No</is_fda_regulated_device>
    <is_us_export>No</is_us_export>
  </oversight_info>
  <brief_summary>
    <textblock>
      Matrix metallopeptidase-9 (MMP-9) expression was compared in healthy and inflamed pulp and&#xD;
      the outcome of coronal pulpotomy in teeth with symptomatic pulpitis was assessed. After&#xD;
      procedure blood samples were examined using Elisa kit.&#xD;
    </textblock>
  </brief_summary>
  <detailed_description>
    <textblock>
      Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp&#xD;
      inflammation stage while improvement of materials, techniques and vital pulp therapy have&#xD;
      received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study&#xD;
      was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term&#xD;
      outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.&#xD;
&#xD;
      Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study.&#xD;
      Coronal pulpotomy was performed using calcium-silicate based material and blood samples were&#xD;
      taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24&#xD;
      hours and 72 hours after the procedure. In control group teeth with healthy pulp were used.&#xD;
      During follow-up visits after three and six-months, patients were examined clinically and&#xD;
      radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and&#xD;
      healthy coronal pulp tissue.&#xD;
    </textblock>
  </detailed_description>
  <overall_status>Completed</overall_status>
  <start_date type="Actual">March 27, 2017</start_date>
  <completion_date type="Actual">February 11, 2018</completion_date>
  <primary_completion_date type="Actual">July 15, 2017</primary_completion_date>
  <phase>N/A</phase>
  <study_type>Interventional</study_type>
  <has_expanded_access>No</has_expanded_access>
  <study_design_info>
    <allocation>Non-Randomized</allocation>
    <intervention_model>Parallel Assignment</intervention_model>
    <primary_purpose>Treatment</primary_purpose>
    <masking>None (Open Label)</masking>
  </study_design_info>
  <primary_outcome>
    <measure>MMP-9 amount measurement using ELISA kit</measure>
    <time_frame>All samples were measured in one time (during 12 hours)</time_frame>
    <description>The MMP-9 amount in the pulp blood samples was measured using the MMP-9 Human ELISA Kit. The measurement procedure was followed according to the manufacturer's instructions. The anti-MMP-9 polyclonal antibody was pre-coated on 96-well plates. Blood samples and a biotin-conjugated antibody were added to the wells supplemented with Avidin-Biotin-Peroxidase Complex and 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. A blue-colored product was produced and turned to yellow after an acidic stop solution was added. The intensity of the color yellow was proportional to the MMP-9 amount bound on the plate. The optical density absorbance was measured spectrophotometrically at 450nm in a microplate reader and the concentration of MMP-9 was calculated. For the expression of MMP-9 concentrations in the samples, a standard curve was used.</description>
  </primary_outcome>
  <number_of_arms>2</number_of_arms>
  <enrollment type="Actual">20</enrollment>
  <condition>Pulpitis</condition>
  <arm_group>
    <arm_group_label>Control group - Healthy teeth</arm_group_label>
    <arm_group_type>Other</arm_group_type>
    <description>Patients with clinically healthy third molar or premolar teeth with indications for extraction; no clinical signs of pulpitis, no caries, no indications for the replacement of an old filling that is 1 mm from the pulp space as determined on the radiograph; a normal response to the cold test, and no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Test group - Teeth with pulpitis</arm_group_label>
    <arm_group_type>Experimental</arm_group_type>
    <description>Patients with symptomatic pulpitis resulting from caries; tooth pain defined as sharp, dull, localized or diffuse; pain at night; a symptomatic tooth with a positive response to the cold test and lingering pain; intermittent or continuous episodes of spontaneous pain (with no external stimulus) that could last from a few minutes up to a few hours; no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.</description>
  </arm_group>
  <intervention>
    <intervention_type>Procedure</intervention_type>
    <intervention_name>Coronal pulpotomy</intervention_name>
    <description>Coronal pulpotomy was performed with a sterile high-speed round bur under water coolant. Pulp vitality was confirmed by the presence of bleeding pulp tissue from all of the canal orifices. Hemostasis was achieved through the application of a pellet moistened with 2.5% NaOCl for two minutes and was repeated as needed. Calcium-silicate based material was prepared ac-cording to the manufacturer's instructions and gently placed over the pulp to fill the entire cavity.</description>
    <arm_group_label>Control group - Healthy teeth</arm_group_label>
    <arm_group_label>Test group - Teeth with pulpitis</arm_group_label>
  </intervention>
  <eligibility>
    <criteria>
      <textblock>
        Inclusion Criteria:&#xD;
&#xD;
          -  Teeth diagnosed with symptomatic pulpitis&#xD;
&#xD;
          -  Patients who did not use any NSAIDs before the treatment&#xD;
&#xD;
          -  Positive cold test of investigated teeth&#xD;
&#xD;
          -  Clinically dental caries in contact with pulp chamber&#xD;
&#xD;
          -  Permanent teeth with radiographically closed root apex&#xD;
&#xD;
        Exclusion Criteria:&#xD;
&#xD;
          -  Patients who refuse to participate in the study&#xD;
&#xD;
          -  Medically compromised patients (with immunosuppressive/systemic diseases, patients on&#xD;
             medications)&#xD;
&#xD;
          -  Teeth with periapical radiolucency or clinical signs of apical periodontitis&#xD;
&#xD;
          -  Periodontological compromised teeth (probing depth ≥4mm)&#xD;
&#xD;
          -  Internal/external root resorption in periapical radiograph&#xD;
&#xD;
          -  Pulp chamber and/or root canal calcification in periapical radiograph&#xD;
&#xD;
          -  Teeth with unrestorable crown&#xD;
&#xD;
          -  Teeth with a negative response to cold test&#xD;
      </textblock>
    </criteria>
    <gender>All</gender>
    <minimum_age>14 Years</minimum_age>
    <maximum_age>33 Years</maximum_age>
    <healthy_volunteers>Accepts Healthy Volunteers</healthy_volunteers>
  </eligibility>
  <overall_official>
    <last_name>Greta Lodiene</last_name>
    <role>Study Director</role>
    <affiliation>Lithuanian University of Health Science</affiliation>
  </overall_official>
  <location>
    <facility>
      <name>Lithuanian University of Health Science</name>
      <address>
        <city>Kaunas</city>
        <country>Lithuania</country>
      </address>
    </facility>
  </location>
  <location_countries>
    <country>Lithuania</country>
  </location_countries>
  <verification_date>January 2019</verification_date>
  <study_first_submitted>November 19, 2018</study_first_submitted>
  <study_first_submitted_qc>January 14, 2019</study_first_submitted_qc>
  <study_first_posted type="Actual">January 17, 2019</study_first_posted>
  <last_update_submitted>January 14, 2019</last_update_submitted>
  <last_update_submitted_qc>January 14, 2019</last_update_submitted_qc>
  <last_update_posted type="Actual">January 17, 2019</last_update_posted>
  <responsible_party>
    <responsible_party_type>Sponsor-Investigator</responsible_party_type>
    <investigator_affiliation>Lithuanian University of Health Sciences</investigator_affiliation>
    <investigator_full_name>Eglė Gvazdaitytė</investigator_full_name>
    <investigator_title>The senior resident of Lithuanian University of Health Sciences</investigator_title>
  </responsible_party>
  <keyword>Calcium-silicate, coronal pulpotomy, MMP-9, symptomatic pulpitis.</keyword>
  <condition_browse>
    <!-- CAUTION:  The following MeSH terms are assigned with an imperfect algorithm            -->
    <mesh_term>Pulpitis</mesh_term>
  </condition_browse>
  <patient_data>
    <sharing_ipd>Undecided</sharing_ipd>
  </patient_data>
  <!-- Results have not yet been posted for this study                                          -->
</clinical_study>


download date: ClinicalTrials.gov processed this data on September 20, 2023
link text: Link to the current ClinicalTrials.gov record.
url: https://clinicaltrials.gov/ct2/show/NCT03807674
org study id: BEC-LSMU(R)-01
nct id: NCT03807674
lead sponsor: 
has dmc: No
is fda regulated drug: No
is fda regulated device: No
is us export: No
Matrix metallopeptidase-9 (MMP-9) expression was compared in healthy and inflamed pulp and
      the outcome of coronal pulpotomy in teeth with symptomatic pulpitis was assessed. After
      procedure blood samples were examined using Elisa kit.
Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp
      inflammation stage while improvement of materials, techniques and vital pulp therapy have
      received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study
      was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term
      outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.

      Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study.
      Coronal pulpotomy was performed using calcium-silicate based material and blood samples were
      taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24
      hours and 72 hours after the procedure. In control group teeth with healthy pulp were used.
      During follow-up visits after three and six-months, patients were examined clinically and
      radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and
      healthy coronal pulp tissue.
allocation: Non-Randomized
intervention model: Parallel Assignment
primary purpose: Treatment
masking: None (Open Label)
measure: MMP-9 amount measurement using ELISA kit
time frame: All samples were measured in one time (during 12 hours)
description: The MMP-9 amount in the pulp blood samples was measured using the MMP-9 Human ELISA Kit. The measurement procedure was followed according to the manufacturer's instructions. The anti-MMP-9 polyclonal antibody was pre-coated on 96-well plates. Blood samples and a biotin-conjugated antibody were added to the wells supplemented with Avidin-Biotin-Peroxidase Complex and 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. A blue-colored product was produced and turned to yellow after an acidic stop solution was added. The intensity of the color yellow was proportional to the MMP-9 amount bound on the plate. The optical density absorbance was measured spectrophotometrically at 450nm in a microplate reader and the concentration of MMP-9 was calculated. For the expression of MMP-9 concentrations in the samples, a standard curve was used.
arm group label: Control group - Healthy teeth
arm group type: Other
description: Patients with clinically healthy third molar or premolar teeth with indications for extraction; no clinical signs of pulpitis, no caries, no indications for the replacement of an old filling that is 1 mm from the pulp space as determined on the radiograph; a normal response to the cold test, and no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.
arm group label: Test group - Teeth with pulpitis
arm group type: Experimental
description: Patients with symptomatic pulpitis resulting from caries; tooth pain defined as sharp, dull, localized or diffuse; pain at night; a symptomatic tooth with a positive response to the cold test and lingering pain; intermittent or continuous episodes of spontaneous pain (with no external stimulus) that could last from a few minutes up to a few hours; no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied.
intervention type: Procedure
intervention name: Coronal pulpotomy
description: Coronal pulpotomy was performed with a sterile high-speed round bur under water coolant. Pulp vitality was confirmed by the presence of bleeding pulp tissue from all of the canal orifices. Hemostasis was achieved through the application of a pellet moistened with 2.5% NaOCl for two minutes and was repeated as needed. Calcium-silicate based material was prepared ac-cording to the manufacturer's instructions and gently placed over the pulp to fill the entire cavity.
arm group label: Control group - Healthy teeth
arm group label: Test group - Teeth with pulpitis
criteria: 
gender: All
minimum age: 14 Years
maximum age: 33 Years
healthy volunteers: Accepts Healthy Volunteers
last name: Greta Lodiene
role: Study Director
affiliation: Lithuanian University of Health Science
facility: 
country: Lithuania
responsible party type: Sponsor-Investigator
investigator affiliation: Lithuanian University of Health Sciences
investigator full name: Eglė Gvazdaitytė
investigator title: The senior resident of Lithuanian University of Health Sciences
mesh term: Pulpitis
sharing ipd: Undecided