<clinical_study>
  <!-- This xml conforms to an XML Schema at:
    https://clinicaltrials.gov/ct2/html/images/info/public.xsd -->
  <required_header>
    <download_date>ClinicalTrials.gov processed this data on September 20, 2023</download_date>
    <link_text>Link to the current ClinicalTrials.gov record.</link_text>
    <url>https://clinicaltrials.gov/ct2/show/NCT03804034</url>
  </required_header>
  <id_info>
    <org_study_id>JCB002</org_study_id>
    <nct_id>NCT03804034</nct_id>
  </id_info>
  <brief_title>Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response</brief_title>
  <official_title>Neurogenic Inflammation and Angiogenesis in the Human Dental Pulp in Response to Occlusal Interferences and Orthodontic Movements</official_title>
  <sponsors>
    <lead_sponsor>
      <agency>Institucion Universitaria Colegios de Colombia</agency>
      <agency_class>Other</agency_class>
    </lead_sponsor>
    <collaborator>
      <agency>Universidad Cooperativa de Colombia seccional Pasto</agency>
      <agency_class>Other</agency_class>
    </collaborator>
  </sponsors>
  <source>Institucion Universitaria Colegios de Colombia</source>
  <oversight_info>
    <has_dmc>Yes</has_dmc>
    <is_fda_regulated_drug>No</is_fda_regulated_drug>
    <is_fda_regulated_device>No</is_fda_regulated_device>
  </oversight_info>
  <brief_summary>
    <textblock>
      This study measures SP, CGRP and VEGF expression in human dental pulp under occlusal trauma&#xD;
      induced by occlusal interferences under moderate orthodontic forces or under a combination of&#xD;
      occlusal trauma and moderate orthodontic forces because in clinical reality, patients under&#xD;
      orthodontic treatment experience a combination of these stimuli on their pulp tissue.&#xD;
    </textblock>
  </brief_summary>
  <detailed_description>
    <textblock>
      Substance P (SP) and calcitonin gene-related peptide (CGRP) are neuropeptides that are&#xD;
      assembled in the cell bodies of the trigeminal nerve and are transported through axonal flow&#xD;
      to the nerve endings in human dental pulp, mainly in type-C nerve fibers and free nerve&#xD;
      endings, where they are released to fulfill important biological functions.&#xD;
&#xD;
      SP plays an important role in pulp tissue reparation because it stimulates angiogenesis using&#xD;
      direct mechanisms by modulating the growth of endothelial cells and fibroblasts through the&#xD;
      activation of growth factors such as VEGF and IGF-1 and stimulates cell migration and&#xD;
      proliferation to form mineralized tissue as a defense mechanism. It also uses indirect&#xD;
      mechanisms such as binding to granulocytes or macrophages, which allow cells with angiogenic&#xD;
      potential to be attracted.&#xD;
&#xD;
      CGRP has a key role in pulp reparation because of its angiogenic potential, which is linked&#xD;
      to endothelial cell stimulation via the cAMP-PKA pathway, promoting cell proliferation. It&#xD;
      boosts VEGF expression and stimulates focal adhesion kinase involved in the stabilization and&#xD;
      maturation of blood vessels. It can stimulate growth factors that act on fibroblasts, such as&#xD;
      HGF, IGF-1, and bFGF, and it can also produce an effect on odontoblast-like cells by&#xD;
      increasing BMP-2 expression and allowing cell proliferation after 24 h. This shows that it&#xD;
      can contribute to mineralized matrix formation&#xD;
&#xD;
      Angiogenesis is mediated by several growth factors, such as the VEGF. VEGF is released in the&#xD;
      pulp tissue as a response to a harmful stimulus to counteract emerging hypoxic areas&#xD;
      regulating oxygen and nutrients supply to cell populations by forming new blood vessels. VEGF&#xD;
      is present in endothelial cells, mast cells, macrophages, lymphocytes, undifferentiated&#xD;
      mesenchymal cells, fibroblasts, and odontoblast-like cells, and it controls neurogenic&#xD;
      inflammation and reparation processes in combination with SP due to its angiogenic potential.&#xD;
&#xD;
      SP, CGRP, VEGF expression can be altered by the masticatory function, occlusal trauma,&#xD;
      orthodontic movements, or a combination of occlusal trauma and orthodontic movements, which&#xD;
      often takes place in patients under orthodontic treatment.&#xD;
    </textblock>
  </detailed_description>
  <overall_status>Completed</overall_status>
  <start_date type="Actual">July 2, 2013</start_date>
  <completion_date type="Actual">January 12, 2015</completion_date>
  <primary_completion_date type="Actual">January 27, 2014</primary_completion_date>
  <phase>N/A</phase>
  <study_type>Interventional</study_type>
  <has_expanded_access>No</has_expanded_access>
  <study_design_info>
    <allocation>Randomized</allocation>
    <intervention_model>Parallel Assignment</intervention_model>
    <intervention_model_description>Human dental pulp was obtained from 40 freshly extracted maxillary and mandibular premolars with orthodontic indication from 20 healthy patients, both male and female, aged between 18 and 30 yearsThe premolars were randomly distributed into four groups, namely a group without occlusal trauma and orthodontic force chosen as the control group and three experimental groups, i.e., one occlusal trauma group experimentally induced by occlusal interferences, one moderate orthodontic force group, and one occlusal trauma plus moderate orthodontic force group.</intervention_model_description>
    <primary_purpose>Basic Science</primary_purpose>
    <masking>None (Open Label)</masking>
  </study_design_info>
  <primary_outcome>
    <measure>SP expression (pmol/mg in pulp tissue)</measure>
    <time_frame>24 hours stimulations</time_frame>
    <description>Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.&#xD;
Results are presented as SP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each neuropeptide were calculated.</description>
  </primary_outcome>
  <secondary_outcome>
    <measure>CGRP expression (pmol/mg in pulp tissue)</measure>
    <time_frame>24 hours stimulations</time_frame>
    <description>Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.&#xD;
Results are presented as CGRP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each neuropeptide were calculated.</description>
  </secondary_outcome>
  <secondary_outcome>
    <measure>VEGF expression (pmol/mg in pulp tissue)</measure>
    <time_frame>24 hours stimulations</time_frame>
    <description>Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.&#xD;
Results are presented as CGRP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each growth factor were calculated.</description>
  </secondary_outcome>
  <number_of_arms>4</number_of_arms>
  <enrollment type="Actual">20</enrollment>
  <condition>Occlusal Trauma</condition>
  <condition>Orthodontic Appliance Complication</condition>
  <arm_group>
    <arm_group_label>Occlusal trauma group</arm_group_label>
    <arm_group_type>Experimental</arm_group_type>
    <description>an occlusal interference was placed on the lower teeth as follows. Articulating paper was used to mark the contact area between the upper and lower premolars indicated for extraction. Once established, the marked area on the lower premolar was acid-etched with 37% phosphoric acid for 15 s, washed, and dried. A bonding agent was placed and light-cured for 15 s, and finally, a 1- to 2-mm block of resin was placed over the contact area and light-cured for 40 s. Articulating paper was used again to verify that only the premolars that were going to be extracted had contact during normal occlusion as well as in lateral movements. Patients were given chewing gum and indications to repeat 20 masticatory cycles for 30 s, followed by a 30-s rest interval, and repeat the sequence again for a period of 30 min. This chewing cycle was repeated three times every 8 h for the first 24 h</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Moderate orthodontic force group</arm_group_label>
    <arm_group_type>Experimental</arm_group_type>
    <description>A convertible standard buccal tube was bonded over the buccal face of the first molar with resin and a McLaughlin, Bennett, and Trevisi slot size 0.022 bracket was bonded over the buccal face of the premolars. One 0.0017 × 0.025 in titanium molybdenum alloy wire cantilever was inserted into each first molar tube, and the wire was bent buccally to form a helix. The cantilever was clinched to the distal end of the tube, and a tipping and extrusive force was applied on the premolar. The activation angle was 45° with a force of 56 g, which was applied to the tooth for 24 h before it was extracted.</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Occlusal trauma and moderate orthodontic force group</arm_group_label>
    <arm_group_type>Experimental</arm_group_type>
    <description>the combination of occlusal trauma and orthodontic force .</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Control Group</arm_group_label>
    <arm_group_type>No Intervention</arm_group_type>
    <description>No experimental device to produce occlusal trauma or orthodontic forces</description>
  </arm_group>
  <intervention>
    <intervention_type>Device</intervention_type>
    <intervention_name>Block of resin</intervention_name>
    <arm_group_label>Occlusal trauma and moderate orthodontic force group</arm_group_label>
    <arm_group_label>Occlusal trauma group</arm_group_label>
  </intervention>
  <intervention>
    <intervention_type>Device</intervention_type>
    <intervention_name>Buccal tube, Bracket and 0.0017 × 0.025 in titanium molybdenum alloy wire</intervention_name>
    <arm_group_label>Moderate orthodontic force group</arm_group_label>
    <arm_group_label>Occlusal trauma and moderate orthodontic force group</arm_group_label>
  </intervention>
  <eligibility>
    <criteria>
      <textblock>
        Inclusion Criteria:&#xD;
&#xD;
          -  Teeth in normal masticatory function&#xD;
&#xD;
          -  Complete root development verified clinically and radiographically&#xD;
&#xD;
        Exclusion Criteria:&#xD;
&#xD;
          -  Patients under medication&#xD;
&#xD;
          -  Smokers&#xD;
&#xD;
          -  Pregnant women were excluded&#xD;
&#xD;
          -  Teeth with caries&#xD;
&#xD;
          -  Teeth with restaurations,&#xD;
&#xD;
          -  Teeth with previous orthodontic treatment&#xD;
&#xD;
          -  Teeth with periodontal disorders&#xD;
&#xD;
          -  Teeth with parafunctional habits.&#xD;
      </textblock>
    </criteria>
    <gender>All</gender>
    <minimum_age>18 Years</minimum_age>
    <maximum_age>30 Years</maximum_age>
    <healthy_volunteers>Accepts Healthy Volunteers</healthy_volunteers>
  </eligibility>
  <location>
    <facility>
      <name>Facultad de Odontologia, Unicoc</name>
      <address>
        <city>Bogota</city>
        <country>Colombia</country>
      </address>
    </facility>
  </location>
  <location_countries>
    <country>Colombia</country>
  </location_countries>
  <reference>
    <citation>Benemei S, Nicoletti P, Capone JG, Geppetti P. CGRP receptors in the control of pain and inflammation. Curr Opin Pharmacol. 2009 Feb;9(1):9-14. doi: 10.1016/j.coph.2008.12.007. Epub 2009 Jan 20.</citation>
    <PMID>19157980</PMID>
  </reference>
  <reference>
    <citation>Caviedes-Bucheli J, Arenas N, Guiza O, Moncada NA, Moreno GC, Diaz E, Munoz HR. Calcitonin gene-related peptide receptor expression in healthy and inflamed human pulp tissue. Int Endod J. 2005 Oct;38(10):712-7. doi: 10.1111/j.1365-2591.2005.01006.x.</citation>
    <PMID>16164685</PMID>
  </reference>
  <reference>
    <citation>Caviedes-Bucheli J, Azuero-Holguin MM, Correa-Ortiz JA, Aguilar-Mora MV, Pedroza-Flores JD, Ulate E, Lombana N, Munoz HR. Effect of experimentally induced occlusal trauma on substance p expression in human dental pulp and periodontal ligament. J Endod. 2011 May;37(5):627-30. doi: 10.1016/j.joen.2011.02.013.</citation>
    <PMID>21496661</PMID>
  </reference>
  <reference>
    <citation>Caviedes-Bucheli J, Gomez-Sosa JF, Azuero-Holguin MM, Ormeno-Gomez M, Pinto-Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017 Apr;50(4):339-351. doi: 10.1111/iej.12627. Epub 2016 Apr 2.</citation>
    <PMID>26953220</PMID>
  </reference>
  <reference>
    <citation>Caviedes-Bucheli J, Lombana N, Azuero-Holguin MM, Munoz HR. Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006 May;39(5):394-400. doi: 10.1111/j.1365-2591.2006.01093.x.</citation>
    <PMID>16640639</PMID>
  </reference>
  <reference>
    <citation>Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. Role of angiogenesis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. J Endod. 2015 Jun;41(6):797-803. doi: 10.1016/j.joen.2014.12.019. Epub 2015 Jan 31.</citation>
    <PMID>25649306</PMID>
  </reference>
  <reference>
    <citation>Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and Function of Hypoxia Inducible Factor-1alpha and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement. Mediators Inflamm. 2015;2015:215761. doi: 10.1155/2015/215761. Epub 2015 Sep 9.</citation>
    <PMID>26441483</PMID>
  </reference>
  <reference>
    <citation>Potente M, Gerhardt H, Carmeliet P. Basic and therapeutic aspects of angiogenesis. Cell. 2011 Sep 16;146(6):873-87. doi: 10.1016/j.cell.2011.08.039.</citation>
    <PMID>21925313</PMID>
  </reference>
  <results_reference>
    <citation>Awawdeh L, Lundy FT, Shaw C, Lamey PJ, Linden GJ, Kennedy JG. Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in pulp tissue from painful and healthy human teeth. Int Endod J. 2002 Jan;35(1):30-6. doi: 10.1046/j.1365-2591.2002.00451.x.</citation>
    <PMID>11853236</PMID>
  </results_reference>
  <verification_date>April 2019</verification_date>
  <study_first_submitted>January 8, 2019</study_first_submitted>
  <study_first_submitted_qc>January 14, 2019</study_first_submitted_qc>
  <study_first_posted type="Actual">January 15, 2019</study_first_posted>
  <last_update_submitted>April 24, 2019</last_update_submitted>
  <last_update_submitted_qc>April 24, 2019</last_update_submitted_qc>
  <last_update_posted type="Actual">April 26, 2019</last_update_posted>
  <responsible_party>
    <responsible_party_type>Principal Investigator</responsible_party_type>
    <investigator_affiliation>Cooperative University of Colombia</investigator_affiliation>
    <investigator_full_name>Javier Caviedes-Bucheli</investigator_full_name>
    <investigator_title>Endodontics postgraduate program professor</investigator_title>
  </responsible_party>
  <keyword>Neurogenic inflammation</keyword>
  <keyword>Dental Pulp</keyword>
  <keyword>Occlusal trauma</keyword>
  <keyword>Orthodontics forces</keyword>
  <keyword>SP</keyword>
  <keyword>CGRP</keyword>
  <keyword>VEGF</keyword>
  <condition_browse>
    <!-- CAUTION:  The following MeSH terms are assigned with an imperfect algorithm            -->
    <mesh_term>Neurogenic Inflammation</mesh_term>
    <mesh_term>Inflammation</mesh_term>
  </condition_browse>
  <intervention_browse>
    <!-- CAUTION:  The following MeSH terms are assigned with an imperfect algorithm            -->
    <mesh_term>Molybdenum</mesh_term>
  </intervention_browse>
  <patient_data>
    <sharing_ipd>Undecided</sharing_ipd>
  </patient_data>
  <!-- Results have not yet been posted for this study                                          -->
</clinical_study>


download date: ClinicalTrials.gov processed this data on September 20, 2023
link text: Link to the current ClinicalTrials.gov record.
url: https://clinicaltrials.gov/ct2/show/NCT03804034
org study id: JCB002
nct id: NCT03804034
lead sponsor: 
collaborator: 
has dmc: Yes
is fda regulated drug: No
is fda regulated device: No
This study measures SP, CGRP and VEGF expression in human dental pulp under occlusal trauma
      induced by occlusal interferences under moderate orthodontic forces or under a combination of
      occlusal trauma and moderate orthodontic forces because in clinical reality, patients under
      orthodontic treatment experience a combination of these stimuli on their pulp tissue.
Substance P (SP) and calcitonin gene-related peptide (CGRP) are neuropeptides that are
      assembled in the cell bodies of the trigeminal nerve and are transported through axonal flow
      to the nerve endings in human dental pulp, mainly in type-C nerve fibers and free nerve
      endings, where they are released to fulfill important biological functions.

      SP plays an important role in pulp tissue reparation because it stimulates angiogenesis using
      direct mechanisms by modulating the growth of endothelial cells and fibroblasts through the
      activation of growth factors such as VEGF and IGF-1 and stimulates cell migration and
      proliferation to form mineralized tissue as a defense mechanism. It also uses indirect
      mechanisms such as binding to granulocytes or macrophages, which allow cells with angiogenic
      potential to be attracted.

      CGRP has a key role in pulp reparation because of its angiogenic potential, which is linked
      to endothelial cell stimulation via the cAMP-PKA pathway, promoting cell proliferation. It
      boosts VEGF expression and stimulates focal adhesion kinase involved in the stabilization and
      maturation of blood vessels. It can stimulate growth factors that act on fibroblasts, such as
      HGF, IGF-1, and bFGF, and it can also produce an effect on odontoblast-like cells by
      increasing BMP-2 expression and allowing cell proliferation after 24 h. This shows that it
      can contribute to mineralized matrix formation

      Angiogenesis is mediated by several growth factors, such as the VEGF. VEGF is released in the
      pulp tissue as a response to a harmful stimulus to counteract emerging hypoxic areas
      regulating oxygen and nutrients supply to cell populations by forming new blood vessels. VEGF
      is present in endothelial cells, mast cells, macrophages, lymphocytes, undifferentiated
      mesenchymal cells, fibroblasts, and odontoblast-like cells, and it controls neurogenic
      inflammation and reparation processes in combination with SP due to its angiogenic potential.

      SP, CGRP, VEGF expression can be altered by the masticatory function, occlusal trauma,
      orthodontic movements, or a combination of occlusal trauma and orthodontic movements, which
      often takes place in patients under orthodontic treatment.
allocation: Randomized
intervention model: Parallel Assignment
intervention model description: Human dental pulp was obtained from 40 freshly extracted maxillary and mandibular premolars with orthodontic indication from 20 healthy patients, both male and female, aged between 18 and 30 yearsThe premolars were randomly distributed into four groups, namely a group without occlusal trauma and orthodontic force chosen as the control group and three experimental groups, i.e., one occlusal trauma group experimentally induced by occlusal interferences, one moderate orthodontic force group, and one occlusal trauma plus moderate orthodontic force group.
primary purpose: Basic Science
masking: None (Open Label)
measure: SP expression (pmol/mg in pulp tissue)
time frame: 24 hours stimulations
description: Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.
Results are presented as SP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each neuropeptide were calculated.
measure: CGRP expression (pmol/mg in pulp tissue)
time frame: 24 hours stimulations
description: Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.
Results are presented as CGRP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each neuropeptide were calculated.
measure: VEGF expression (pmol/mg in pulp tissue)
time frame: 24 hours stimulations
description: Sample Collection: All the teeth involved in this study were anesthetized and extracted 5 min after anesthetic application with conventional methods. Immediately after extraction, teeth were sectioned. Pulp tissue was obtained using a sterile endodontic excavator, placed on an Eppendorf tube, snap frozen in liquid nitrogen until the radioimmunoassay (RIA) test was performed.
Results are presented as CGRP expression in pmol/mg of pulp tissue. Means and standard deviations as well as minimum and maximum values for each growth factor were calculated.
arm group label: Occlusal trauma group
arm group type: Experimental
description: an occlusal interference was placed on the lower teeth as follows. Articulating paper was used to mark the contact area between the upper and lower premolars indicated for extraction. Once established, the marked area on the lower premolar was acid-etched with 37% phosphoric acid for 15 s, washed, and dried. A bonding agent was placed and light-cured for 15 s, and finally, a 1- to 2-mm block of resin was placed over the contact area and light-cured for 40 s. Articulating paper was used again to verify that only the premolars that were going to be extracted had contact during normal occlusion as well as in lateral movements. Patients were given chewing gum and indications to repeat 20 masticatory cycles for 30 s, followed by a 30-s rest interval, and repeat the sequence again for a period of 30 min. This chewing cycle was repeated three times every 8 h for the first 24 h
arm group label: Moderate orthodontic force group
arm group type: Experimental
description: A convertible standard buccal tube was bonded over the buccal face of the first molar with resin and a McLaughlin, Bennett, and Trevisi slot size 0.022 bracket was bonded over the buccal face of the premolars. One 0.0017 × 0.025 in titanium molybdenum alloy wire cantilever was inserted into each first molar tube, and the wire was bent buccally to form a helix. The cantilever was clinched to the distal end of the tube, and a tipping and extrusive force was applied on the premolar. The activation angle was 45° with a force of 56 g, which was applied to the tooth for 24 h before it was extracted.
arm group label: Occlusal trauma and moderate orthodontic force group
arm group type: Experimental
description: the combination of occlusal trauma and orthodontic force .
arm group label: Control Group
arm group type: No Intervention
description: No experimental device to produce occlusal trauma or orthodontic forces
intervention type: Device
intervention name: Block of resin
arm group label: Occlusal trauma and moderate orthodontic force group
arm group label: Occlusal trauma group
intervention type: Device
intervention name: Buccal tube, Bracket and 0.0017 × 0.025 in titanium molybdenum alloy wire
arm group label: Moderate orthodontic force group
arm group label: Occlusal trauma and moderate orthodontic force group
criteria: 
gender: All
minimum age: 18 Years
maximum age: 30 Years
healthy volunteers: Accepts Healthy Volunteers
facility: 
country: Colombia
citation: Benemei S, Nicoletti P, Capone JG, Geppetti P. CGRP receptors in the control of pain and inflammation. Curr Opin Pharmacol. 2009 Feb;9(1):9-14. doi: 10.1016/j.coph.2008.12.007. Epub 2009 Jan 20.
PMID: 19157980
citation: Caviedes-Bucheli J, Arenas N, Guiza O, Moncada NA, Moreno GC, Diaz E, Munoz HR. Calcitonin gene-related peptide receptor expression in healthy and inflamed human pulp tissue. Int Endod J. 2005 Oct;38(10):712-7. doi: 10.1111/j.1365-2591.2005.01006.x.
PMID: 16164685
citation: Caviedes-Bucheli J, Azuero-Holguin MM, Correa-Ortiz JA, Aguilar-Mora MV, Pedroza-Flores JD, Ulate E, Lombana N, Munoz HR. Effect of experimentally induced occlusal trauma on substance p expression in human dental pulp and periodontal ligament. J Endod. 2011 May;37(5):627-30. doi: 10.1016/j.joen.2011.02.013.
PMID: 21496661
citation: Caviedes-Bucheli J, Gomez-Sosa JF, Azuero-Holguin MM, Ormeno-Gomez M, Pinto-Pascual V, Munoz HR. Angiogenic mechanisms of human dental pulp and their relationship with substance P expression in response to occlusal trauma. Int Endod J. 2017 Apr;50(4):339-351. doi: 10.1111/iej.12627. Epub 2016 Apr 2.
PMID: 26953220
citation: Caviedes-Bucheli J, Lombana N, Azuero-Holguin MM, Munoz HR. Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006 May;39(5):394-400. doi: 10.1111/j.1365-2591.2006.01093.x.
PMID: 16640639
citation: Saghiri MA, Asatourian A, Sorenson CM, Sheibani N. Role of angiogenesis in endodontics: contributions of stem cells and proangiogenic and antiangiogenic factors to dental pulp regeneration. J Endod. 2015 Jun;41(6):797-803. doi: 10.1016/j.joen.2014.12.019. Epub 2015 Jan 31.
PMID: 25649306
citation: Wei F, Yang S, Xu H, Guo Q, Li Q, Hu L, Liu D, Wang C. Expression and Function of Hypoxia Inducible Factor-1alpha and Vascular Endothelial Growth Factor in Pulp Tissue of Teeth under Orthodontic Movement. Mediators Inflamm. 2015;2015:215761. doi: 10.1155/2015/215761. Epub 2015 Sep 9.
PMID: 26441483
citation: Potente M, Gerhardt H, Carmeliet P. Basic and therapeutic aspects of angiogenesis. Cell. 2011 Sep 16;146(6):873-87. doi: 10.1016/j.cell.2011.08.039.
PMID: 21925313
citation: Awawdeh L, Lundy FT, Shaw C, Lamey PJ, Linden GJ, Kennedy JG. Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in pulp tissue from painful and healthy human teeth. Int Endod J. 2002 Jan;35(1):30-6. doi: 10.1046/j.1365-2591.2002.00451.x.
PMID: 11853236
responsible party type: Principal Investigator
investigator affiliation: Cooperative University of Colombia
investigator full name: Javier Caviedes-Bucheli
investigator title: Endodontics postgraduate program professor
mesh term: Neurogenic Inflammation
mesh term: Inflammation
mesh term: Molybdenum
sharing ipd: Undecided