<clinical_study>
  <!-- This xml conforms to an XML Schema at:
    https://clinicaltrials.gov/ct2/html/images/info/public.xsd -->
  <required_header>
    <download_date>ClinicalTrials.gov processed this data on September 20, 2023</download_date>
    <link_text>Link to the current ClinicalTrials.gov record.</link_text>
    <url>https://clinicaltrials.gov/ct2/show/NCT01663116</url>
  </required_header>
  <id_info>
    <org_study_id>Cx611-0101</org_study_id>
    <nct_id>NCT01663116</nct_id>
  </id_info>
  <brief_title>Cx611-0101, eASCs Intravenous Administration to Refractory Rheumatoid Arthritis Patients</brief_title>
  <official_title>&quot;Phase Ib/IIa, Escalating Dose, Single Blind, Clinical Trial to Assess the Safety of the i.v Administration of Allogeneic Adipose-derived Mesenchymal Cells (eASCs) to Refractory Rheumatoid Arthritis (RA) Patients&quot;.</official_title>
  <sponsors>
    <lead_sponsor>
      <agency>Tigenix S.A.U.</agency>
      <agency_class>Industry</agency_class>
    </lead_sponsor>
  </sponsors>
  <source>Takeda</source>
  <oversight_info>
    <has_dmc>Yes</has_dmc>
  </oversight_info>
  <brief_summary>
    <textblock>
      Phase Ib/IIa clinical trial of a new medicinal product of the somatic cell therapy class&#xD;
      (eASCs). This study is designed as a multicenter, single blind, fixed dose escalation, with&#xD;
      three treatment groups, controlled with placebo (randomization 3:1) whose target population&#xD;
      are patients with rheumatoid arthritis refractory to at least two biologic.&#xD;
    </textblock>
  </brief_summary>
  <detailed_description>
    <textblock>
      Traditionally, RA has been treated with non-steroidal anti-inflammatory drugs,&#xD;
      glucocorticoids and non-biologics-DMARDs. Only non-biologics-DMARDs and, at a lesser extent,&#xD;
      glucocorticoids have shown to be able to prevent or interrupt the inflammatory and&#xD;
      destructive disease processes.&#xD;
&#xD;
      Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are able to&#xD;
      differentiate into mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, and&#xD;
      adipose. MSCs can be easily isolated from bone marrow or adipose tissue and rapidly expanded&#xD;
      in culture. MSCs have also been shown to have immuno-suppressive and healing capacities,&#xD;
      improve angiogenesis and prevent fibrosis. These properties could be used for novel&#xD;
      therapeutic applications in various disorders, including rheumatoid arthritis, osteoarthritis&#xD;
      (OA), genetic bone and cartilage disorders and bone metastasis. MSCs can potently modulate&#xD;
      immune responses, showing antiproliferative and anti-inflammatory capacities.&#xD;
&#xD;
      This study is a multicenter phase Ib/IIa, escalating dose, single blind clinical trial to&#xD;
      assess the safety of the intravenous administration of expanded allogeneic adipose-derived&#xD;
      mesenchymal stem cells (eASCs) to refractory rheumatoid arthritis (RA) patients.&#xD;
&#xD;
      The primary objective of the study is to determine the safety, feasibility and tolerance, and&#xD;
      to identify, if possible, the dose limiting toxicity (DLT) and the dose for future clinical&#xD;
      trials on efficacy of the intravenous infusion of allogeneic eASCs for patients suffering&#xD;
      rheumatoid arthritis (RA) under treatment with at least one non-biologic-Disease modifying&#xD;
      antirheumatoid drug (DMARD) who have previously failed to treatment with at least two&#xD;
      biologics. The secondary objective is to obtain information on the clinical and functional&#xD;
      effects of the intravenous infusion of allogeneic eASCs in patients with RA and to explore&#xD;
      pharmacodynamics parameters.&#xD;
&#xD;
      53 patients (i.e. patients having received at least one dose of study treatment) in three&#xD;
      different cohorts are planned to be included in this clinical trial. Expansion will start&#xD;
      after acute toxicity assessment of the first three patients of each cohort.&#xD;
&#xD;
      Dose and intervals for the trial consist of the following active groups: a) first cohort: 1&#xD;
      million cells/kg administered at days 1, 8 and 15; b) second cohort: 2 million cells / kg&#xD;
      administered at days 1, 8 and 15; c) third cohort: 4 million cells / kg administered at days&#xD;
      1, 8 and 15.&#xD;
    </textblock>
  </detailed_description>
  <overall_status>Completed</overall_status>
  <start_date>March 2011</start_date>
  <completion_date type="Actual">January 2013</completion_date>
  <primary_completion_date type="Actual">January 2013</primary_completion_date>
  <phase>Phase 1/Phase 2</phase>
  <study_type>Interventional</study_type>
  <has_expanded_access>No</has_expanded_access>
  <study_design_info>
    <allocation>Randomized</allocation>
    <intervention_model>Parallel Assignment</intervention_model>
    <primary_purpose>Treatment</primary_purpose>
    <masking>Single (Participant)</masking>
  </study_design_info>
  <primary_outcome>
    <measure>Number of Adverse Events and Severe Adverse Events</measure>
    <time_frame>6 months follow up after the first administration</time_frame>
    <description>Total number of Adverse Events and Severe Adverse Events, related and non-related with the medication will be recorded as a measure of tolerability and safety.</description>
  </primary_outcome>
  <secondary_outcome>
    <measure>Proportion of ACR20 patients/ACR50 patients/ACR70 patients (swollen joints*, tender joints**, physician global assessment***, patient global assessment***, patient´s assessment of pain ***, ESR/CRP, HAQ score)</measure>
    <time_frame>At selection and screening visit, and once per month during 6 months after the last administration</time_frame>
  </secondary_outcome>
  <number_of_arms>2</number_of_arms>
  <enrollment type="Actual">53</enrollment>
  <condition>Rheumatoid Arthritis Aggravated</condition>
  <arm_group>
    <arm_group_label>Treatment</arm_group_label>
    <arm_group_type>Experimental</arm_group_type>
    <description>first cohort: 1 million stem cells/kg administered at days 1, 8 and 15&#xD;
second cohort: 2 million stem cells / kg administered at days 1, 8 and 15&#xD;
third cohort: 4 million stem cells / kg administered at days 1, 8 and 15</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Placebo</arm_group_label>
    <arm_group_type>Placebo Comparator</arm_group_type>
    <description>Lactate Ringer´s solution</description>
  </arm_group>
  <intervention>
    <intervention_type>Genetic</intervention_type>
    <intervention_name>Stem cells</intervention_name>
    <description>first cohort: 1 million stem cells/kg administered at days 1, 8 and 15&#xD;
second cohort: 2 million stem cells / kg administered at days 1, 8 and 15&#xD;
third cohort: 4 million stem cells / kg administered at days 1, 8 and 15</description>
    <arm_group_label>Treatment</arm_group_label>
  </intervention>
  <intervention>
    <intervention_type>Genetic</intervention_type>
    <intervention_name>Placebo</intervention_name>
    <description>first cohort: 20 ml administered at days 1, 8 and 15&#xD;
second cohort: 40 ml administered at days 1, 8 and 15&#xD;
third cohort: 80 ml administered at days 1, 8 and 15</description>
    <arm_group_label>Placebo</arm_group_label>
  </intervention>
  <eligibility>
    <criteria>
      <textblock>
        Inclusion criteria&#xD;
&#xD;
        Patients must meet all the following inclusion criteria to be eligible for study entry:&#xD;
&#xD;
          1. Must understand and voluntarily sign an informed consent form prior to the conduct of&#xD;
             any study related assessment/procedures.&#xD;
&#xD;
          2. Subjects with RA under treatment with at least one non-biologic-DMARD and failure to&#xD;
             treatment with at least two biologics.&#xD;
&#xD;
          3. Of either gender, aged ≥ 18 years at time of consent.&#xD;
&#xD;
          4. Able to adhere to the study visit schedule and other protocol requirements.&#xD;
&#xD;
          5. Have a diagnosis of RA for ≥6 months.&#xD;
&#xD;
          6. EULAR DAS28-ESR activity criteria &gt;3.2.&#xD;
&#xD;
          7. Four tender joints to palpation and four swollen joints, based on a 68/66-joint count.&#xD;
&#xD;
          8. Be receiving treatment on an outpatient basis.&#xD;
&#xD;
          9. If taking methotrexate, leflunomide, or sulfasalazine, must have been treated for at&#xD;
             least 16 weeks and on a stable dose (oral methotrexate ≤ 25 mg/week; parenteral&#xD;
             methotrexate ≤ 20 mg/week; leflunomide ≤ 20 mg/day; sulfasalazine ≤ 3 g/day) for at&#xD;
             least 4 weeks prior to the start of treatment and throughout the study. (See also&#xD;
             section 7.5). The rest of the DMARDs (gold salts, etc) should be maintained at stable&#xD;
             doses during at least the 4 weeks prior to the start of treatment and throughout the&#xD;
             study (see also section 7.5).&#xD;
&#xD;
         10. If taking oral corticosteroids, must be on a stable dose of prednisone ≤ 10 mg/day or&#xD;
             equivalent for at least 1 month prior to screening. ( See also section 7.5).&#xD;
&#xD;
         11. If taking NSAIDs, must be on stable dose for at least 2 weeks prior to screening and&#xD;
             until they have completed the Week 24 study visit. (See also section 7.5).&#xD;
&#xD;
         12. Male subjects (including those who have had a vasectomy) must agree to use barrier&#xD;
             contraception (latex condoms) when engaging in activity in which conception is&#xD;
             possible while on study medication and for at least 28 days after taking the last dose&#xD;
             of study medication.&#xD;
&#xD;
         13. Females of Childbearing Potential* must have a negative urine pregnancy test at&#xD;
             Screening and Baseline and must be willing to use one medically approved form of birth&#xD;
             control when engaging in activity in which conception is possible while on study&#xD;
             medication and for at least 28 days after taking the last dose of study medication.&#xD;
&#xD;
               -  A female of childbearing potential is a sexually mature female who 1) has not&#xD;
                  undergone a hysterectomy (the surgical removal of the uterus) or bilateral&#xD;
                  ovariectomy (the surgical removal of both ovaries) or 2) has not been&#xD;
                  postmenopausal for at least 24 consecutive months (i.e., has had menses at any&#xD;
                  time during the preceding 24 consecutive months).&#xD;
&#xD;
        Exclusion criteria&#xD;
&#xD;
        A patient CANNOT be recruited into this study if any of the following criteria is met:&#xD;
&#xD;
          1. Treatment with biologics within the following period prior to the start of treatment:&#xD;
&#xD;
               1. Infliximab: 8 weeks&#xD;
&#xD;
               2. Etanercept: 2 weeks&#xD;
&#xD;
               3. Adalimumab and certolizumab: 4 weeks&#xD;
&#xD;
               4. Abatacept, tocilizumab and golimumab: 8 weeks&#xD;
&#xD;
               5. Rituximab: 6 months&#xD;
&#xD;
               6. Anakinra: 3 days No treatment with biologics is allowed during the first 12 weeks&#xD;
                  after the start of the study treatment. Thus, the patients should have complied&#xD;
                  with the periods indicated above, and should not receive any biologics during the&#xD;
                  period specified.&#xD;
&#xD;
          2. Presence of a severe bleeding or thrombotic disorder.&#xD;
&#xD;
          3. History of known pulmonary embolism or known secondary anti-phospholipid syndrome.&#xD;
&#xD;
          4. Received any of the following treatments within 2 years prior to study entry:&#xD;
             anti-cancer therapy (e.g. alkylating agents, anti-metabolites, purine analogues,&#xD;
             monoclonal antibodies for malignancy).&#xD;
&#xD;
          5. Received within 4 weeks prior to the start of treatment: intra-articular,&#xD;
             intramuscular or intravenous corticosteroids. (See also section 7.5).&#xD;
&#xD;
          6. Past or current malignant melanoma.&#xD;
&#xD;
          7. Past or current malignancy; except for in situ cervical cancer, non-invasive basal&#xD;
             cell and squamous cell skin carcinoma, superficial bladder tumors (Ta and Tis) with a&#xD;
             complete response duration of &gt;10 years. In the case of lymphoma or breast cancer&#xD;
             patients will be allowed to participate in the trial with a complete response duration&#xD;
             of &gt;20 years.&#xD;
&#xD;
          8. Other autoimmune diseases, previous or current inflammatory joint disease other than&#xD;
             rheumatoid arthritis, currently active or previous recurrent bacterial, viral, fungal,&#xD;
             or other infections including, but not limited to, tuberculosis and atypical&#xD;
             mycobacterial disease, clinically significant abnormalities on chest radiograph,&#xD;
             hepatitis B and C, and recurrent herpes zoster.&#xD;
&#xD;
          9. Chronic or ongoing active infectious disease requiring systemic treatment such as, but&#xD;
             not limited to, chronic renal infection, chronic chest infection with bronchiectasis,&#xD;
             Mycobacterium tuberculosis infection (TB) and active hepatitis B and C.&#xD;
&#xD;
             For the screening of latent TB, the results of the tests performed in the last year&#xD;
             according to the usual practice of the center or local guidelines will be accepted, as&#xD;
             long as the investigator rules out a situation of high contact risk in the months&#xD;
             after the last screening in accordance with the medical history of the patient.&#xD;
&#xD;
             If the patient has a recent chest X-ray (performed in the month prior to enrollment in&#xD;
             the study) and there is no clinical evidence or history suggestive of recent contact,&#xD;
             it will not be necessary to repeat the test.&#xD;
&#xD;
         10. Subjects with signs of latent TB can be included if they have started treatment&#xD;
             according to local guidelines at least one month prior to starting investigational&#xD;
             therapy.&#xD;
&#xD;
         11. Clinically significant cardiac disease including unstable angina, acute myocardial&#xD;
             infarction within six months from screening, congestive heart failure of worse than&#xD;
             grade II of the New York criteria.&#xD;
&#xD;
         12. Significant concurrent, uncontrolled medical condition including, but not limited to,&#xD;
             renal, hepatic, hematological, gastrointestinal, endocrine, pulmonary, neurological,&#xD;
             cerebral psychiatric disease, or evidence of demyelinating disease.&#xD;
&#xD;
         13. History of significant cerebrovascular disease.&#xD;
&#xD;
         14. Subjects with congenital or acquired immunodeficiencies.&#xD;
&#xD;
         15. Known human immunodeficiency virus (HIV) positive.&#xD;
&#xD;
         16. Screening laboratory values (according to central laboratory):&#xD;
&#xD;
               -  Haemoglobin &lt;5.6 mmol/L (9.0 g/dL).&#xD;
&#xD;
               -  Neutrophils &lt;1.5 x 10(9)/L.&#xD;
&#xD;
               -  Leukocytes &lt;3.0 x 10(9)/L.&#xD;
&#xD;
               -  Platelets &lt;100 x 10(9)/L.&#xD;
&#xD;
               -  Serum IgG &lt;lower limit of normal (LLN).&#xD;
&#xD;
               -  Alanine amino transferase (ALT) &gt; 1.5 times the upper limit of normal (ULN).&#xD;
&#xD;
               -  Total bilirubin &gt;2 mg/dl.&#xD;
&#xD;
               -  Aspartate amino transferase (AST) &gt;1.5 times ULN.&#xD;
&#xD;
               -  Alkaline phosphatase (ALP) &gt;2 times ULN.&#xD;
&#xD;
               -  Creatinine &gt;133 mmol/L (1.5 mg/dL).&#xD;
&#xD;
         17. Serologic evidence of hepatitis C (HC) infection.&#xD;
&#xD;
         18. Serologic evidence of hepatitis B (HB) infection based on the results of testing for&#xD;
             HBsAg, anti-HBc and anti-HBs antibodies as follows:&#xD;
&#xD;
               -  Subjects positive for HBsAg are excluded.&#xD;
&#xD;
               -  Subjects negative for HBsAg but positive for both anti-HBc and anti-HBs&#xD;
                  antibodies were eligible to participate.&#xD;
&#xD;
               -  Subjects negative for HBsAg and anti-HBc antibody but positive for anti-HBs&#xD;
                  antibody are eligible to participate.&#xD;
&#xD;
               -  Subjects negative for HBsAg and anti-HBs antibody but positive for anti-HBc&#xD;
                  antibody required clarification of their status by testing for HB DNA which if&#xD;
                  positive excludes the subject from participation.&#xD;
&#xD;
                    -  Patients with documented vaccination against hepatitis B (primary and&#xD;
                       secondary immunization and booster) are considered negative.&#xD;
&#xD;
         19. Receipt of any vaccination (live, attenuated or killed) in 8 wks prior to baseline.&#xD;
&#xD;
         20. Subjects who had received treatment with any non-marketed drug substance or&#xD;
             experimental therapy within 4 weeks prior to screening.&#xD;
&#xD;
         21. Current participation in any other interventional clinical study.&#xD;
&#xD;
         22. Subjects known or suspected of not being able to comply with a study protocol (e.g.&#xD;
             due to alcoholism, drug dependency or psychological disorder).&#xD;
&#xD;
         23. MRI is unfeasible, (e.g. due to the presence of pacemakers, hip replacements or severe&#xD;
             claustrophobia).&#xD;
&#xD;
         24. Subjects with impossibility of having a radiological exploration.&#xD;
&#xD;
         25. Known allergies or hypersensitivity to antibiotics, HSA, DMEM, materials of bovine&#xD;
             origin, gadolinium (MRI contrast) and Ringer's Lactate Solution.&#xD;
&#xD;
         26. Pregnancy and breastfeeding.&#xD;
&#xD;
         27. Any other condition which the PI judges would make patient unsuitable for study&#xD;
             participation.&#xD;
      </textblock>
    </criteria>
    <gender>All</gender>
    <minimum_age>18 Years</minimum_age>
    <maximum_age>N/A</maximum_age>
    <healthy_volunteers>No</healthy_volunteers>
  </eligibility>
  <overall_official>
    <last_name>José María Alvaro-Gracia, MD, PhD</last_name>
    <role>Study Chair</role>
    <affiliation>Hospital de la Princesa</affiliation>
  </overall_official>
  <location>
    <facility>
      <name>Hospital U. Central de Asturias</name>
      <address>
        <city>Oviedo</city>
        <state>Asturias</state>
        <zip>33006</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital General de Mérida</name>
      <address>
        <city>Mérida</city>
        <state>Badajoz</state>
        <zip>06800</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. de Canarias</name>
      <address>
        <city>La Laguna</city>
        <state>Tenerife</state>
        <zip>38320</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital de Cruces</name>
      <address>
        <city>Barakaldo</city>
        <state>Vizcaya</state>
        <zip>48903</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital de Basurto</name>
      <address>
        <city>Bilbao</city>
        <state>Vizcaya</state>
        <zip>48013</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Complejo Hospitalario U. A Coruña</name>
      <address>
        <city>A Coruña</city>
        <zip>15006</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Vall d'Hebron</name>
      <address>
        <city>Barcelona</city>
        <zip>08035</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Clinic i Provincial</name>
      <address>
        <city>Barcelona</city>
        <zip>08036</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Santa Creu i Sant Pau</name>
      <address>
        <city>Barcelona</city>
        <zip>08041</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Reina Sofía</name>
      <address>
        <city>Córdoba</city>
        <zip>14004</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Clínico San Cecilio</name>
      <address>
        <city>Granada</city>
        <zip>18012</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. Virgen de las Nieves</name>
      <address>
        <city>Granada</city>
        <zip>18014</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. de Guadalajara</name>
      <address>
        <city>Guadalajara</city>
        <zip>19002</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital de La Princesa</name>
      <address>
        <city>Madrid</city>
        <zip>28006</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. Gregorio Marañon</name>
      <address>
        <city>Madrid</city>
        <zip>28007</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Clínico San Carlos</name>
      <address>
        <city>Madrid</city>
        <zip>28040</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. La Paz</name>
      <address>
        <city>Madrid</city>
        <zip>28046</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Carlos Haya</name>
      <address>
        <city>Málaga</city>
        <zip>29010</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital U. Marques de Valdecilla</name>
      <address>
        <city>Santander</city>
        <zip>39008</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Ntra. Sª de Valme</name>
      <address>
        <city>Sevilla</city>
        <zip>41014</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Virgen Macarena</name>
      <address>
        <city>Sevilla</city>
        <zip>41071</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital La Fe</name>
      <address>
        <city>Valencia</city>
        <zip>46009</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location>
    <facility>
      <name>Hospital Dr. Peset</name>
      <address>
        <city>Valencia</city>
        <zip>46017</zip>
        <country>Spain</country>
      </address>
    </facility>
  </location>
  <location_countries>
    <country>Spain</country>
  </location_countries>
  <verification_date>April 2019</verification_date>
  <study_first_submitted>August 5, 2011</study_first_submitted>
  <study_first_submitted_qc>August 10, 2012</study_first_submitted_qc>
  <study_first_posted type="Estimate">August 13, 2012</study_first_posted>
  <last_update_submitted>April 10, 2019</last_update_submitted>
  <last_update_submitted_qc>April 10, 2019</last_update_submitted_qc>
  <last_update_posted type="Actual">April 12, 2019</last_update_posted>
  <responsible_party>
    <responsible_party_type>Sponsor</responsible_party_type>
  </responsible_party>
  <keyword>Rheumatoid arthritis</keyword>
  <keyword>Expanded adipose derived allogeneic adult stem cells (eASCs)</keyword>
  <condition_browse>
    <!-- CAUTION:  The following MeSH terms are assigned with an imperfect algorithm            -->
    <mesh_term>Arthritis</mesh_term>
    <mesh_term>Arthritis, Rheumatoid</mesh_term>
  </condition_browse>
  <!-- Results have not yet been posted for this study                                          -->
</clinical_study>


download date: ClinicalTrials.gov processed this data on September 20, 2023
link text: Link to the current ClinicalTrials.gov record.
url: https://clinicaltrials.gov/ct2/show/NCT01663116
org study id: Cx611-0101
nct id: NCT01663116
lead sponsor: 
has dmc: Yes
Phase Ib/IIa clinical trial of a new medicinal product of the somatic cell therapy class
      (eASCs). This study is designed as a multicenter, single blind, fixed dose escalation, with
      three treatment groups, controlled with placebo (randomization 3:1) whose target population
      are patients with rheumatoid arthritis refractory to at least two biologic.
Traditionally, RA has been treated with non-steroidal anti-inflammatory drugs,
      glucocorticoids and non-biologics-DMARDs. Only non-biologics-DMARDs and, at a lesser extent,
      glucocorticoids have shown to be able to prevent or interrupt the inflammatory and
      destructive disease processes.

      Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are able to
      differentiate into mesenchymal tissues such as bone, cartilage, muscle, ligament, tendon, and
      adipose. MSCs can be easily isolated from bone marrow or adipose tissue and rapidly expanded
      in culture. MSCs have also been shown to have immuno-suppressive and healing capacities,
      improve angiogenesis and prevent fibrosis. These properties could be used for novel
      therapeutic applications in various disorders, including rheumatoid arthritis, osteoarthritis
      (OA), genetic bone and cartilage disorders and bone metastasis. MSCs can potently modulate
      immune responses, showing antiproliferative and anti-inflammatory capacities.

      This study is a multicenter phase Ib/IIa, escalating dose, single blind clinical trial to
      assess the safety of the intravenous administration of expanded allogeneic adipose-derived
      mesenchymal stem cells (eASCs) to refractory rheumatoid arthritis (RA) patients.

      The primary objective of the study is to determine the safety, feasibility and tolerance, and
      to identify, if possible, the dose limiting toxicity (DLT) and the dose for future clinical
      trials on efficacy of the intravenous infusion of allogeneic eASCs for patients suffering
      rheumatoid arthritis (RA) under treatment with at least one non-biologic-Disease modifying
      antirheumatoid drug (DMARD) who have previously failed to treatment with at least two
      biologics. The secondary objective is to obtain information on the clinical and functional
      effects of the intravenous infusion of allogeneic eASCs in patients with RA and to explore
      pharmacodynamics parameters.

      53 patients (i.e. patients having received at least one dose of study treatment) in three
      different cohorts are planned to be included in this clinical trial. Expansion will start
      after acute toxicity assessment of the first three patients of each cohort.

      Dose and intervals for the trial consist of the following active groups: a) first cohort: 1
      million cells/kg administered at days 1, 8 and 15; b) second cohort: 2 million cells / kg
      administered at days 1, 8 and 15; c) third cohort: 4 million cells / kg administered at days
      1, 8 and 15.
allocation: Randomized
intervention model: Parallel Assignment
primary purpose: Treatment
masking: Single (Participant)
measure: Number of Adverse Events and Severe Adverse Events
time frame: 6 months follow up after the first administration
description: Total number of Adverse Events and Severe Adverse Events, related and non-related with the medication will be recorded as a measure of tolerability and safety.
measure: Proportion of ACR20 patients/ACR50 patients/ACR70 patients (swollen joints*, tender joints**, physician global assessment***, patient global assessment***, patient´s assessment of pain ***, ESR/CRP, HAQ score)
time frame: At selection and screening visit, and once per month during 6 months after the last administration
arm group label: Treatment
arm group type: Experimental
description: first cohort: 1 million stem cells/kg administered at days 1, 8 and 15
second cohort: 2 million stem cells / kg administered at days 1, 8 and 15
third cohort: 4 million stem cells / kg administered at days 1, 8 and 15
arm group label: Placebo
arm group type: Placebo Comparator
description: Lactate Ringer´s solution
intervention type: Genetic
intervention name: Stem cells
description: first cohort: 1 million stem cells/kg administered at days 1, 8 and 15
second cohort: 2 million stem cells / kg administered at days 1, 8 and 15
third cohort: 4 million stem cells / kg administered at days 1, 8 and 15
arm group label: Treatment
intervention type: Genetic
intervention name: Placebo
description: first cohort: 20 ml administered at days 1, 8 and 15
second cohort: 40 ml administered at days 1, 8 and 15
third cohort: 80 ml administered at days 1, 8 and 15
arm group label: Placebo
criteria: 
gender: All
minimum age: 18 Years
maximum age: N/A
healthy volunteers: No
last name: José María Alvaro-Gracia, MD, PhD
role: Study Chair
affiliation: Hospital de la Princesa
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country: Spain
responsible party type: Sponsor
mesh term: Arthritis
mesh term: Arthritis, Rheumatoid