<clinical_study>
  <!-- This xml conforms to an XML Schema at:
    https://clinicaltrials.gov/ct2/html/images/info/public.xsd -->
  <required_header>
    <download_date>ClinicalTrials.gov processed this data on September 20, 2023</download_date>
    <link_text>Link to the current ClinicalTrials.gov record.</link_text>
    <url>https://clinicaltrials.gov/ct2/show/NCT01720771</url>
  </required_header>
  <id_info>
    <org_study_id>EPN-2011/530</org_study_id>
    <secondary_id>EPN-2011/530</secondary_id>
    <nct_id>NCT01720771</nct_id>
  </id_info>
  <brief_title>Preventive Effect of a Probiotic Tablet on Oral Health in Preschool Children</brief_title>
  <official_title>Preventive Effect of a Probiotic Tablet on Oral Health in Preschool Children</official_title>
  <sponsors>
    <lead_sponsor>
      <agency>University of Copenhagen</agency>
      <agency_class>Other</agency_class>
    </lead_sponsor>
  </sponsors>
  <source>University of Copenhagen</source>
  <oversight_info>
    <has_dmc>No</has_dmc>
  </oversight_info>
  <brief_summary>
    <textblock>
      The aim of the prospective project is to investigate effect of daily probiotic supplements on&#xD;
      dental caries development in preschool children living in a low socioeconomic immigrant area&#xD;
      in souithern Sweden. The study design is a randomised controlled trial with two parallell&#xD;
      arms. The intervention is one probiotic tablet or placebo once daily. The null hypothesis is&#xD;
      that the caries experience and increment will not differ betwwen the two groups.&#xD;
    </textblock>
  </brief_summary>
  <detailed_description>
    <textblock>
      Background In spite of the general decline in dental caries, early childhood caries (ECC) is&#xD;
      still a severe global problem with social and behavioural gradients [1, 2]. The disease is&#xD;
      multi-factorial but the key event is a pH-induced stress in the oral environment that results&#xD;
      in reduced diversity of the oral biofilm [3]. The biofilm simply adapt to this situation with&#xD;
      an overgrowth of caries-associated aciduric strains on behalf of beneficial and health&#xD;
      promoting bacteria. Therefore, the addition of probiotic bacteria has been suggested as a&#xD;
      novel approach to maintain oral health as an adjunct to the existing evidence-based methods&#xD;
      [4, 5]. Previous clinical trials have indicated that a daily intake of lactobacilli-derived&#xD;
      dairy products can reduce caries incidence, improve general health and diminish the use of&#xD;
      antibiotics in preschool children [6, 7, 8]. However, further studies on the probiotic&#xD;
      concept are needed; all children do not drink milk and no studies on childhood caries have&#xD;
      hitherto been conducted with non-dairy products and streptococci strains.&#xD;
&#xD;
      Objectives The aim of the present project is to evaluate the effect of daily administration&#xD;
      of chewing tablets containing probiotic bacteria (Probiora3) on plaque amount, gingival&#xD;
      health and caries incidence in preschool children. In addition, information on general health&#xD;
      will be collected. The null hypothesis is that the oral health will not differ from preschool&#xD;
      children with a similar intake of placebo tablets. An additional aim is to monitor the&#xD;
      microbial composition in saliva samples at baseline and during the course of intervention.&#xD;
&#xD;
      Study design The study design is a prospective randomised, double-blind, placebo-controlled&#xD;
      trial with two parallel arms. The duration is 12 months. Assignment to intervention and&#xD;
      control group will be made by a computer-based randomization in groups A or B.&#xD;
&#xD;
      Material The study group will be recruited among the 2-year-old children that are regular&#xD;
      patients attending the Public Dental Clinic in Rosengård, Malmö, Sweden. The clinic is&#xD;
      situated in a multicultural suburban area with a low socio-economy. The proportion of&#xD;
      immigrants is high in the area, the majority Arabic speaking. The inclusion criteria are i)&#xD;
      uncompromised general health, ii) no cavities or severe dental diseases, and iii) not&#xD;
      mentally or physically disabled. Children that are uncooperative or totally reject dental&#xD;
      examination are excluded. The children will be consecutively enrolled after signed informed&#xD;
      consent (verbal and written) by their parents and assigned to either the test or the control&#xD;
      group by pre-numbered envelopes generated by a computer program. As a new patient is entering&#xD;
      the study, the clinician takes the next numbered envelope assigning the patient to either&#xD;
      group A or B. The protocol has been subjected to the regional ethical committee and is&#xD;
      approved.&#xD;
&#xD;
      Power calculation A power calculation of the clinical scores with alpha set at 0.05 and beta&#xD;
      = 0.2 estimates that 85 subjects in each arm is needed to disclose a 30% difference between&#xD;
      the treatment groups with respect to the clinical scores (Fleiss Statistical Methods for&#xD;
      rates and Proportions, 2nd edition, John Wiley and Sons New York 1981). Due to expected&#xD;
      relocations and failure to comply with the protocol, the goal will be to recruit a minimum of&#xD;
      220 and a maximum of 250 children.&#xD;
&#xD;
      Intervention and duration All families will receive comprehensive reinforcement of the oral&#xD;
      health message by their dental hygienists prior to baseline. The focus will be on daily&#xD;
      supervised brushing with fluoride toothpaste. At the baseline examination, plaque index,&#xD;
      bleeding after brushing and caries are scored on special forms for optical reading. Data on&#xD;
      ongoing medication etc will be extracted from the dental records. In addition, a caries risk&#xD;
      assessment is carried out with the aid of a computerized program (Cariogram) according to&#xD;
      Stecksén-Blicks et al. 2007 [8]. The parents of the children in the test-group (group A) will&#xD;
      be instructed to give their children one EvoraKids® tablet once daily, ideally after&#xD;
      brushing, for a period of 12 months. The parents of the children in the control-group (group&#xD;
      B) will receive the same instructions for the intake of tablets without live probiotic&#xD;
      bacteria. The group assignment will be unknown both for the parents and the investigators and&#xD;
      disclosed only after the statistical calculations. The allocation concealment is kept by the&#xD;
      manufacturer. All parents are encouraged to maintain all their habitual oral hygiene routines&#xD;
      and use fluoride toothpaste throughout the study period.&#xD;
&#xD;
      The test tablets contain Probiora3 which is a trade mark for three probiotic streptococci&#xD;
      strains (S. uberis KJ2, S. oralis KJ3 and S. rattus JH145) at a concentration of 3x108 CFU.&#xD;
      The tablets are sweetened by erythritrol which is a tooth-friendly polyol (for nutrition&#xD;
      facts, see Appendix B). The chew tablets (test and placebo), identical in taste and&#xD;
      appearance, will be supplied by the manufacturer (Oragenics, USA) in packages separated by&#xD;
      the letter &quot;A&quot; or &quot;B&quot;. The participating families will be provided with tablets for three&#xD;
      month's use at the time and the compliance will be checked and registered at each follow-up.&#xD;
      Parents will be instructed to report any perceived side- or adverse-effects immediately to&#xD;
      the dental staffs and stop the intake.&#xD;
&#xD;
      Clinical examinations&#xD;
&#xD;
      Clinical examinations will be performed at baseline and after 6 and 12 months. The families&#xD;
      are contacted every third month for interviews and delivery of study products. The&#xD;
      examinations and scoring will be carried out visually and tactile by two well-trained and&#xD;
      calibrated dental hygienists at the dental clinic. At baseline, information on the domestic&#xD;
      oral hygiene routines, use of baby bottles and habitual sweet dietary intakes will be&#xD;
      collected by a 10-question questionnaire. The following endpoints are scored:&#xD;
&#xD;
      Primary outcome measures:&#xD;
&#xD;
        -  visible plaque index (VPI)&#xD;
&#xD;
        -  prevalence of gingival inflammation (bleeding after brushing, BAB)&#xD;
&#xD;
        -  prevalence of caries (cavitated lesions and white spot lesions)&#xD;
&#xD;
      Secondary outcome measures:&#xD;
&#xD;
        -  number of upper or lower respiratory infections&#xD;
&#xD;
        -  number of otitis media&#xD;
&#xD;
        -  number of days with diarrhea&#xD;
&#xD;
        -  doctor's visits&#xD;
&#xD;
        -  prescription of antibiotics&#xD;
&#xD;
        -  incidence of allergy, asthma or eczema&#xD;
&#xD;
      Detailed methodology Visible plaque index (VPI): Presence or absence of dental plaque along&#xD;
      the gingival margin of the upper incisors and canines Bleeding after brushing (BAB):&#xD;
      Presence/absence of bleeding after gentle brushing with a soft toothbrush Presence of caries:&#xD;
      Cavitated and non-cavitated lesions are registered in all teeth according to the criterion of&#xD;
      the Public Dental Service and expressed as dmft and dmfs&#xD;
&#xD;
      The secondary endpoints are scored every sixth month through telephone calls to the parents&#xD;
      and based on parental logbooks.&#xD;
&#xD;
      Clinical sampling In order to monitor the oral microbiota in the 2 study groups during the&#xD;
      course of the study, saliva samples will be collected at baseline (before intervention) and&#xD;
      at the 6 and 12 months follow-ups according to Barfod and co-workers [9]. A cotton bud is&#xD;
      placed by the parents in the mouth of their child under supervision of the clinical staff.&#xD;
      When soaked, the bud will be transferred to a small plastic tube, marked with the patients&#xD;
      study number, and immediately frozen and stored. After the full completion of the trial, all&#xD;
      samples will be shipped on expense of the manufacturer to Oragenics, FL, USA for further&#xD;
      handling and processing. The running costs for the clinical samplings are covered by a&#xD;
      separate agreement.&#xD;
&#xD;
      Statistical methods All data are processed with the IBM-SPSS software (version 19.0, Chicago&#xD;
      IL, USA). The categorical data are subjected chi-square tests. Continuous measurements will&#xD;
      be calculated with aid of non-parametric tests. The caries incidence is calculated as the&#xD;
      difference in dmfs/dmft between follow-up and baseline. Comparisons are carried out between&#xD;
      the study groups at baseline and at the follow-ups. The group concealment will not be&#xD;
      unveiled until after the statistical calculations. A p value less than 0.05 is considered as&#xD;
      statistically significant.&#xD;
&#xD;
      Report of results The null hypothesis will either be rejected or accepted. The findings will&#xD;
      without any unnecessary delay be reported at an international congress and submitted for&#xD;
      publication to an appropriate peer-reviewed scientific journal. The suppliers will have a the&#xD;
      possibility to read and comment on the manuscript before submission.&#xD;
&#xD;
      Ethical considerations There are no side- or adverse-effects to be foreseen in connection&#xD;
      with the intervention. All the patients are managed by dentists or specially trained dental&#xD;
      hygienists according to &quot;best clinical practice&quot; during and after the project. A rapid&#xD;
      development of caries will be managed by non-operative and operative treatment irrespective&#xD;
      of group. All treatment will be carried out without costs for the families. The tablets are&#xD;
      distributed free of charge. All adverse events related to the treatment provided will be&#xD;
      recorded on an adverse event form. The investigation will be performed according to the&#xD;
      principles of the Declaration of Helsinki on experimentation involving human subjects.&#xD;
    </textblock>
  </detailed_description>
  <overall_status>Completed</overall_status>
  <start_date>February 2013</start_date>
  <completion_date type="Actual">May 2015</completion_date>
  <primary_completion_date type="Actual">December 2014</primary_completion_date>
  <phase>N/A</phase>
  <study_type>Interventional</study_type>
  <has_expanded_access>No</has_expanded_access>
  <study_design_info>
    <allocation>Randomized</allocation>
    <intervention_model>Parallel Assignment</intervention_model>
    <primary_purpose>Prevention</primary_purpose>
    <masking>Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)</masking>
  </study_design_info>
  <primary_outcome>
    <measure>Caries increment in primary teeth</measure>
    <time_frame>after 1 year of intervention</time_frame>
    <description>Caries is recorded by clinical examinations with mirror and explorer</description>
  </primary_outcome>
  <number_of_arms>2</number_of_arms>
  <enrollment type="Actual">138</enrollment>
  <condition>Dental Caries</condition>
  <arm_group>
    <arm_group_label>Probiotic tablet</arm_group_label>
    <arm_group_type>Active Comparator</arm_group_type>
    <description>a tablet with three probiotic streptococci strains (S. uberis KJ2, S. oralis KJ3 and S. rattus JH145) at a concentration of 3x108 CFU</description>
  </arm_group>
  <arm_group>
    <arm_group_label>Sugar pill</arm_group_label>
    <arm_group_type>Placebo Comparator</arm_group_type>
    <description>The same tablet but without active probiotic bacteria</description>
  </arm_group>
  <intervention>
    <intervention_type>Dietary Supplement</intervention_type>
    <intervention_name>probiotic tablet</intervention_name>
    <description>Intake of one probiotic lozenge tablet per day</description>
    <arm_group_label>Probiotic tablet</arm_group_label>
    <other_name>Evora Kids</other_name>
  </intervention>
  <intervention>
    <intervention_type>Dietary Supplement</intervention_type>
    <intervention_name>sugar pill</intervention_name>
    <description>one tablet daily</description>
    <arm_group_label>Sugar pill</arm_group_label>
    <other_name>Evora kids (placebo)</other_name>
  </intervention>
  <eligibility>
    <criteria>
      <textblock>
        Inclusion Criteria:)&#xD;
&#xD;
          -  uncompromised general health,&#xD;
&#xD;
          -  no cavities or severe dental diseases,&#xD;
&#xD;
          -  and not mentally or physically disabled&#xD;
&#xD;
        Exclusion Criteria:&#xD;
&#xD;
          -  Children that are uncooperative or totally reject dental examination are excluded&#xD;
      </textblock>
    </criteria>
    <gender>All</gender>
    <minimum_age>2 Years</minimum_age>
    <maximum_age>3 Years</maximum_age>
    <healthy_volunteers>Accepts Healthy Volunteers</healthy_volunteers>
  </eligibility>
  <location>
    <facility>
      <name>Public Dental Clinic, Folktandvården Rosengård, Wachtmeistersväg 13</name>
      <address>
        <city>Malmö</city>
        <state>Skåne</state>
        <zip>213 73</zip>
        <country>Sweden</country>
      </address>
    </facility>
  </location>
  <location_countries>
    <country>Sweden</country>
  </location_countries>
  <verification_date>May 2015</verification_date>
  <study_first_submitted>October 31, 2012</study_first_submitted>
  <study_first_submitted_qc>November 1, 2012</study_first_submitted_qc>
  <study_first_posted type="Estimate">November 2, 2012</study_first_posted>
  <last_update_submitted>May 28, 2015</last_update_submitted>
  <last_update_submitted_qc>May 28, 2015</last_update_submitted_qc>
  <last_update_posted type="Estimate">May 29, 2015</last_update_posted>
  <responsible_party>
    <responsible_party_type>Principal Investigator</responsible_party_type>
    <investigator_affiliation>University of Copenhagen</investigator_affiliation>
    <investigator_full_name>Svante Twetman</investigator_full_name>
    <investigator_title>Professor</investigator_title>
  </responsible_party>
  <keyword>bacteriotherapy</keyword>
  <keyword>caries</keyword>
  <keyword>preschool children</keyword>
  <keyword>probiotics</keyword>
  <condition_browse>
    <!-- CAUTION:  The following MeSH terms are assigned with an imperfect algorithm            -->
    <mesh_term>Dental Caries</mesh_term>
  </condition_browse>
  <!-- Results have not yet been posted for this study                                          -->
</clinical_study>


download date: ClinicalTrials.gov processed this data on September 20, 2023
link text: Link to the current ClinicalTrials.gov record.
url: https://clinicaltrials.gov/ct2/show/NCT01720771
org study id: EPN-2011/530
secondary id: EPN-2011/530
nct id: NCT01720771
lead sponsor: 
has dmc: No
The aim of the prospective project is to investigate effect of daily probiotic supplements on
      dental caries development in preschool children living in a low socioeconomic immigrant area
      in souithern Sweden. The study design is a randomised controlled trial with two parallell
      arms. The intervention is one probiotic tablet or placebo once daily. The null hypothesis is
      that the caries experience and increment will not differ betwwen the two groups.
Background In spite of the general decline in dental caries, early childhood caries (ECC) is
      still a severe global problem with social and behavioural gradients [1, 2]. The disease is
      multi-factorial but the key event is a pH-induced stress in the oral environment that results
      in reduced diversity of the oral biofilm [3]. The biofilm simply adapt to this situation with
      an overgrowth of caries-associated aciduric strains on behalf of beneficial and health
      promoting bacteria. Therefore, the addition of probiotic bacteria has been suggested as a
      novel approach to maintain oral health as an adjunct to the existing evidence-based methods
      [4, 5]. Previous clinical trials have indicated that a daily intake of lactobacilli-derived
      dairy products can reduce caries incidence, improve general health and diminish the use of
      antibiotics in preschool children [6, 7, 8]. However, further studies on the probiotic
      concept are needed; all children do not drink milk and no studies on childhood caries have
      hitherto been conducted with non-dairy products and streptococci strains.

      Objectives The aim of the present project is to evaluate the effect of daily administration
      of chewing tablets containing probiotic bacteria (Probiora3) on plaque amount, gingival
      health and caries incidence in preschool children. In addition, information on general health
      will be collected. The null hypothesis is that the oral health will not differ from preschool
      children with a similar intake of placebo tablets. An additional aim is to monitor the
      microbial composition in saliva samples at baseline and during the course of intervention.

      Study design The study design is a prospective randomised, double-blind, placebo-controlled
      trial with two parallel arms. The duration is 12 months. Assignment to intervention and
      control group will be made by a computer-based randomization in groups A or B.

      Material The study group will be recruited among the 2-year-old children that are regular
      patients attending the Public Dental Clinic in Rosengård, Malmö, Sweden. The clinic is
      situated in a multicultural suburban area with a low socio-economy. The proportion of
      immigrants is high in the area, the majority Arabic speaking. The inclusion criteria are i)
      uncompromised general health, ii) no cavities or severe dental diseases, and iii) not
      mentally or physically disabled. Children that are uncooperative or totally reject dental
      examination are excluded. The children will be consecutively enrolled after signed informed
      consent (verbal and written) by their parents and assigned to either the test or the control
      group by pre-numbered envelopes generated by a computer program. As a new patient is entering
      the study, the clinician takes the next numbered envelope assigning the patient to either
      group A or B. The protocol has been subjected to the regional ethical committee and is
      approved.

      Power calculation A power calculation of the clinical scores with alpha set at 0.05 and beta
      = 0.2 estimates that 85 subjects in each arm is needed to disclose a 30% difference between
      the treatment groups with respect to the clinical scores (Fleiss Statistical Methods for
      rates and Proportions, 2nd edition, John Wiley and Sons New York 1981). Due to expected
      relocations and failure to comply with the protocol, the goal will be to recruit a minimum of
      220 and a maximum of 250 children.

      Intervention and duration All families will receive comprehensive reinforcement of the oral
      health message by their dental hygienists prior to baseline. The focus will be on daily
      supervised brushing with fluoride toothpaste. At the baseline examination, plaque index,
      bleeding after brushing and caries are scored on special forms for optical reading. Data on
      ongoing medication etc will be extracted from the dental records. In addition, a caries risk
      assessment is carried out with the aid of a computerized program (Cariogram) according to
      Stecksén-Blicks et al. 2007 [8]. The parents of the children in the test-group (group A) will
      be instructed to give their children one EvoraKids® tablet once daily, ideally after
      brushing, for a period of 12 months. The parents of the children in the control-group (group
      B) will receive the same instructions for the intake of tablets without live probiotic
      bacteria. The group assignment will be unknown both for the parents and the investigators and
      disclosed only after the statistical calculations. The allocation concealment is kept by the
      manufacturer. All parents are encouraged to maintain all their habitual oral hygiene routines
      and use fluoride toothpaste throughout the study period.

      The test tablets contain Probiora3 which is a trade mark for three probiotic streptococci
      strains (S. uberis KJ2, S. oralis KJ3 and S. rattus JH145) at a concentration of 3x108 CFU.
      The tablets are sweetened by erythritrol which is a tooth-friendly polyol (for nutrition
      facts, see Appendix B). The chew tablets (test and placebo), identical in taste and
      appearance, will be supplied by the manufacturer (Oragenics, USA) in packages separated by
      the letter "A" or "B". The participating families will be provided with tablets for three
      month's use at the time and the compliance will be checked and registered at each follow-up.
      Parents will be instructed to report any perceived side- or adverse-effects immediately to
      the dental staffs and stop the intake.

      Clinical examinations

      Clinical examinations will be performed at baseline and after 6 and 12 months. The families
      are contacted every third month for interviews and delivery of study products. The
      examinations and scoring will be carried out visually and tactile by two well-trained and
      calibrated dental hygienists at the dental clinic. At baseline, information on the domestic
      oral hygiene routines, use of baby bottles and habitual sweet dietary intakes will be
      collected by a 10-question questionnaire. The following endpoints are scored:

      Primary outcome measures:

        -  visible plaque index (VPI)

        -  prevalence of gingival inflammation (bleeding after brushing, BAB)

        -  prevalence of caries (cavitated lesions and white spot lesions)

      Secondary outcome measures:

        -  number of upper or lower respiratory infections

        -  number of otitis media

        -  number of days with diarrhea

        -  doctor's visits

        -  prescription of antibiotics

        -  incidence of allergy, asthma or eczema

      Detailed methodology Visible plaque index (VPI): Presence or absence of dental plaque along
      the gingival margin of the upper incisors and canines Bleeding after brushing (BAB):
      Presence/absence of bleeding after gentle brushing with a soft toothbrush Presence of caries:
      Cavitated and non-cavitated lesions are registered in all teeth according to the criterion of
      the Public Dental Service and expressed as dmft and dmfs

      The secondary endpoints are scored every sixth month through telephone calls to the parents
      and based on parental logbooks.

      Clinical sampling In order to monitor the oral microbiota in the 2 study groups during the
      course of the study, saliva samples will be collected at baseline (before intervention) and
      at the 6 and 12 months follow-ups according to Barfod and co-workers [9]. A cotton bud is
      placed by the parents in the mouth of their child under supervision of the clinical staff.
      When soaked, the bud will be transferred to a small plastic tube, marked with the patients
      study number, and immediately frozen and stored. After the full completion of the trial, all
      samples will be shipped on expense of the manufacturer to Oragenics, FL, USA for further
      handling and processing. The running costs for the clinical samplings are covered by a
      separate agreement.

      Statistical methods All data are processed with the IBM-SPSS software (version 19.0, Chicago
      IL, USA). The categorical data are subjected chi-square tests. Continuous measurements will
      be calculated with aid of non-parametric tests. The caries incidence is calculated as the
      difference in dmfs/dmft between follow-up and baseline. Comparisons are carried out between
      the study groups at baseline and at the follow-ups. The group concealment will not be
      unveiled until after the statistical calculations. A p value less than 0.05 is considered as
      statistically significant.

      Report of results The null hypothesis will either be rejected or accepted. The findings will
      without any unnecessary delay be reported at an international congress and submitted for
      publication to an appropriate peer-reviewed scientific journal. The suppliers will have a the
      possibility to read and comment on the manuscript before submission.

      Ethical considerations There are no side- or adverse-effects to be foreseen in connection
      with the intervention. All the patients are managed by dentists or specially trained dental
      hygienists according to "best clinical practice" during and after the project. A rapid
      development of caries will be managed by non-operative and operative treatment irrespective
      of group. All treatment will be carried out without costs for the families. The tablets are
      distributed free of charge. All adverse events related to the treatment provided will be
      recorded on an adverse event form. The investigation will be performed according to the
      principles of the Declaration of Helsinki on experimentation involving human subjects.
allocation: Randomized
intervention model: Parallel Assignment
primary purpose: Prevention
masking: Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
measure: Caries increment in primary teeth
time frame: after 1 year of intervention
description: Caries is recorded by clinical examinations with mirror and explorer
arm group label: Probiotic tablet
arm group type: Active Comparator
description: a tablet with three probiotic streptococci strains (S. uberis KJ2, S. oralis KJ3 and S. rattus JH145) at a concentration of 3x108 CFU
arm group label: Sugar pill
arm group type: Placebo Comparator
description: The same tablet but without active probiotic bacteria
intervention type: Dietary Supplement
intervention name: probiotic tablet
description: Intake of one probiotic lozenge tablet per day
arm group label: Probiotic tablet
other name: Evora Kids
intervention type: Dietary Supplement
intervention name: sugar pill
description: one tablet daily
arm group label: Sugar pill
other name: Evora kids (placebo)
criteria: 
gender: All
minimum age: 2 Years
maximum age: 3 Years
healthy volunteers: Accepts Healthy Volunteers
facility: 
country: Sweden
responsible party type: Principal Investigator
investigator affiliation: University of Copenhagen
investigator full name: Svante Twetman
investigator title: Professor
mesh term: Dental Caries