How could click reaction take place at low concentration?

I have DBCO-functionalized resin, and the DBCO group is validated with FAM-azide.


But I cannot use this resin to react with FAM-Azide-labled primer, which has very low concentration (20 μM, it is already very concentrate at DNA-scale).


I think that might be caused by concentration, because the reaction between DBCO-resin and FAM-azide is also not significant at such low concentration.


I have already tried long reaction time and higher temperature.
How could I get this work?


