How to calculate absolute charge of a protein [closed]








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My understanding is that hydrogen bonds formed by proteins require the NH2 / COOH to be neutral. Hence to find out when the hydrogen bonds are the strongest will depend on the absolute charge of the protein and not the net charge. Hence how do you calculate this absolute charge or is there another way to determine when the strongest hydrogen bonds will be formed when changing the pH condition?


Note: For absolute charge I do not need the actual charge in coulombs but more of the relative charge the NH2 / COOH will bear when changing the pH conditions


