+ R 1 COOH R 2 COOH R 3 COOH R n COOMe FAME + + H 2 O CH 2 OH CHOH CH 2 OH R n COOH Extracellular Tll CH 2 OCOR 1 CHOCOR 2 CH 2 OCOR 3 TAG FFA Glycerol FFA MeOH + FAME + H 2 O RnCOOH FFA

Intracellular Tll RnCOOMe

Recombinant yeast (n=1, 2, 3,4)