Mediated Alleviation of Dormancy of Arabidopsis Seeds Possibly via the Modulation of Expression of Genes Related to Light, GA, and ABA


Supplementary Table

. Effect of different combinations of pre-or post-incubations in darkness on BL light effect on germination rates of WT dormant Arabidopsis Col-0 seeds. Results are expressed as % of germinated seeds after 48 and 72 h of incubation in specific light conditions ± SD. Experiments were conducted in three biological and two technical replicates (100 seeds per each replicate). 

Supplementary Table S2. Characteristic of primers used in qRT-PCR analysis of genes relative expression


Gene

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gene name Gene accession no. NCBI number L/R Sequence 5' --> 3' Tm o C Product size [bp] Reference genes

  
	HYH	HY5-Homolog	At3g17609	NM_180274.2	L R	CACAAGAAGCACAAAACTGAGG TGCGCTGATACTCTGTTCCT	53.0 51.8
	LAF1	Long After Far-Red Light 1	At4g25560	NM_118688.3	L R	CCCCTCCTTCGTCTTCATCA AGCCTTGGAATCTCAGGAGC	58.8 59.4
	ACT7 CIB1 APC2 CIB5 HBT SUB1 PP7	Actin 7 Cryptochrome Interacting Basic Helix-Loop-Helix 1 Anaphase-Promoting Complex/Cyclosome 2 Cryptochrome Interacting Basic Helix-Loop-Helix 5 Hobbit Calcium Ion Binding Protein SUB1 Serine/Threonine Phosphatase 7	At5g09810 At4g34530 At2g04660 At1g26260 At2g20000 At4g08810 Light signaling related genes L L NM_119618.3 R NM_121018.3 R L L NM_202184.2 R NM_126495.2 R NM_179663.2 L R L NM_116951.1 R At5g63870 L NM_125782.3 R	GGAGCTGAGAGATTCCGTTG CACAGCATAGCAGAACGAGT GGCCTTGGATTCACAATTGC TCTTTGCTCATACGGTCAGC GGAGGAGTTAAGCGCAATCA TCGATGAAACTTTCGGCTGT TGAGGAAATGTTCGGTTGCA CACCAACTTGTCATGTCCCA ATCGATGAGAGCCCGTGAAA GGCTCTTGTGGCTGTGTTAC GAAGGGCAAGCATCGAAGTA CTCCACACCTGAACCAATCC CTGGTCTTGGTGGAATGGAC GAGGATCCGAGAAATCAGGC	58.1 58.0 57.9 57.9 57.9 57.8 58.0 58.0 59.2 57.9 58.1 58.1 58.1 57.8
	PHYA PHR2	Phytochrome A Photolyase/Blue Light Receptor 2	At1g09570 At2g47590	NM_100828.3 NM_130327.3	L R L R	CAAGGTCCGGATGATAGTTG AACTACAACCGCCATAACCA ATCGCAGCGCTTGATCAATT CTGGTTTCCCTTGCTCAACC	51.8 49.7 58.9 59.0
	PHYB COP1	Phytochrome B Constitutive Photomorphogenic 1	At2g18790 At2g32950	NM_127435.3 NM_128855.3	L R L R	AAAGAATCTGAGGCGGCTAT CTGCAATCTTAGCGTTCCAT TCTCACAGTGAACAGCGAGT ATTCGCAGTCAACATCGTGG	49.7 49.7 58.9 58.9
	CRY1	Cryptochrome 1	At4g08920	NM_116961.4	L R	CAGCAGCGGAAGGAGAGAAA TTTCACCCGGAGTTACAGCCC	60.0 59.9
	CRY2	Cryptochrome 2	At1g04400	NM_100320.3	L R	TTCGGGGAAATAAGCGTCAG TCAACATCAGCATCCCAAGG	57.9 57.9
	CRY3	Cryptochrome 3	At5g24850	NM_122394.3	L R	GGAGAGATGCCAAGACAGGG TTGTGCTTGCTTGGGAATGC	59.8 59.9
	HY5	Elongated Hypocotyl 5	At5g11260	NM_121164.4	L R	CGGAGAAAGAGAACAAGCGG CCACCACCTCCTCTCTTGTT	58.9 58.9
	HFR1	Long Hypocotyl in Far-Red	At1g02340	NM_100115.3	L R	CTAAATCCGGCGAATCACAT GGAACCAAACCGTGAAGAGA	49.7 51.8

Genes involved in ABA metabolism and signalling

  
	ABI4 RGL1 ABI5 RGL2 HAI1 RGL3 HAI2 XERICO HAI3 KAO2	ABA Insensitive 4 RGA-Like 1 ABA Insensitive 5 RGA-Like 2 Highly ABA Induced PP2C Gene 1 RGA-Like 3 Highly ABA Induced PP2C Gene 2 RING/U-box superfamily protein Highly ABA Induced PP2C Gene 3 Arabidopsis thaliana Ent-kaurenoic acid axidase 2	At2G40220 At1g66350 At2G36270 At3g03450 At5g59220 At5g17490 At1g07430 At2g04240 At2g29380 At2g32440	NM_105306.3 NM_129580.1 NM_111216.2 NM_129185.3 NM_121755.2 NM_125312.3 NM_201687.1 NM_100617.2 NM_128492.2 NM_001202728	R L L R R L L R R L L R R L L R R R L L R	CACCAGAAGAGTCGTCACAG GTTAGGGCAGGAACAAGGAG AAGCATGCTCTCGGATCTTG AGAGCGCGTAGAGGATAACT TATAACCCGGATCCAGACCC ATGATCAAGAACCGCGAGTC ACCGGAGTTAGACTTGTCCA CGGTAAATACGACGAGCCAA ATCAATGTCCGCAATCTCCC GTCACGAGCCATTGGAGATA ACTTACACTCTCCAGGTCCC GTTATCGTCTCCACCACCAC AACACATTCGAACGACGCTA ATGTCACGAGCCATAGGAGA GCAAGAACAGGCAGACAA GGCACAACAACAAGAGGAG TCTTCTTCACTCCTCCTCCC CTCTTCTTCTACCGCACACC CCGAGATTCCAAACGGTGAT CAGGGAAGCAAAGAGTGATG CAAACCAAAAGGAAGGAATG	57.9 57.8 51.8 51.8 57.9 58.1 51.8 51.8 58.0 57.1 53.8 53.8 57.9 57.9 48.9 51.1 57.7 53.8 51.8 58.4 57.1
			Genes involved in GA metabolism and signaling	
	GA3ox1	Gibberellin 3-Oxidase 1	At1g15550	NM_101424.2	L R	CTTGGGGTGCCTTCCAAATC AACCTTCGGACCACATTTGC	59.1 59.0
	GA3ox2	Gibberellin 3-Oxidase 2	At1g80340	NM_106683.1	L R	CCAAGTTGATGTGGTTCGCA GAGGGTCGAGTCTGTATGGG	59.0 58.9
	ABA1 GA2ox6 NCED6 GA2ox2 NCED9 GID1a CYP707A2 GID1b ABI3 GID1c	ABA Deficient 1 Gibberellin 2-Oxidase 6 Nine-Cis-Epoxycarotenoid Dioxygenase 6 Gibberellin 2-Oxidase 2 Nine-Cis-Epoxycarotenoid Dioxygenase 9 GA Insensitive Dwarf1A Cytochrome P450, Family 707, Subfamily A, Polypeptide 2 GA Insensitive Dwarf1B ABA Insensitive 3 GA Insensitive Dwarf1C	At5g67030 At1g02400 At3g24220 At1g30040 At1g78390 At3g05120 At2g29090 At3g63010 At3G24650 At5g27320	NM_100121.4 NM_126103.2 NM_001036035.1 NM_113327.2 NM_111384.3 NM_106486.2 NM_116166.4 NM_001202700 NM_113376.3 NM_122614.3	L L R R L L R R L L R R L L R R L L R	TGGCTGCAATTATGGCTTCC AGCGAAGTGAGTGAGCTGAT GGGTCAGATGGGATTGGGAT TCGGCTTTGTCAGTGAGTCT AATGCGTGGGAAGAGAGAAC ATCCCCACATACAAACCGGT GCAAGCCGAAGAAGCCAATA GGTTTATGTGACCCGCTTCT CAATGATAGCCCACCCGAAA TACTCTTTGTCGCAGGCTTG CCGATTCACCCGCTCTTAAA ACCTCTTATCATCTCCGGCA GCAGCAAGTGTACTGTCCTT CCTCGAGCTAACCAAACCTC GACAACAACAACACCGCAAA GCTCACTTCCTGGACATGAG CATCTCCAGCTCCTGTCAAC TGTGCATAATGTCGCGGTAA ATGCTCTCTGTCTTCACCCT	59.1 58.8 51.8 51.8 59.0 58.9 57.9 57.9 51.8 51.8 57.9 57.9 53.8 49.7 58.1 57.9 57.9 49.7 51.8
Marlena Stawska, Krystyna Oracz
Mediated Alleviation of Dormancy of Arabidopsis Seeds Possibly via the Modulation of Expression of Genes Related to Light, GA, and ABA