Genetic mapping with testcrossing association and F2:3 populations reveals the importance of heterosis in chilling tolerance at maize seedling stage


a The positions of the candidate genes were based on the public maize genome data set B73 RefGen_v2. 
b The homologs of the candidate genes in red have been reported associating with stress tolerance in Arabidopsis or rice. 

Supplemental table 3 Sequences of the primers used for gene expression assay


Gene name

Forward primer (5′-3′) Reverse primer (5′-3′) 
GRMZM2G403609 ACACCCATGATGACGATGGT CTCTTCAGCTGGTCCCTCAA GRMZM2G000936 GCGAGGAAGGATTGCAGAAG ACCAGCTCTGTTGTTCCGTA GRMZM2G058518 CACCGGTGGAAAACTCAAGG GCACCTCAAAGTTAGGCACC GRMZM2G463462 GCTGAGAGGGATGAATGGGA GGAGCTGCTGAATCCAATGG GRMZM2G051917 AGCCTTGGATCTGTTGCTCT TCTTGTTGTGTGCGGACTTG GRMZM2G035807 GGAGACAGGACACAACAGGA TGATCCGCCATATCCTCCAC GRMZM2G012479 ATGGCTCCCTCGTTCACTAG TTTGCTGTTGTCAACCCACA GRMZM2G092327 CAACTCCTGGTCGCTTGATG TCCTGTTGTGTCCTGTCTCC GRMZM2G138161 ATTTGTGCCACCTCAACCAC TGACAAGCGATGGAAGAGGT GRMZM2G019986 TCCCCATCCACAGAGCTTTT ATCCGAACCTGACCCATTGT Actin1/GRMZM2G126010
TACGAGATGCCTGATGGTCAGGTCA TGGAGTTGTACGTGGCCTCATGGAC 



  


Supplementary figure, table and data file legends


Supplementary Figure

Figure S1 Performance of chilling tolerance in Mei C, K932 and their hybrid A Leaf rolling degree in the two parents and their hybrid in the field experiment of 2014 winter B Leaf rolling degree in the two parents and their hybrid in the experiment of growth chamber Supplementary Tables Supplemental table 1 Annotation and category of the candidate genes predicted according to the significant loci revealed by GWAS across all the testcrosses Supplemental table 2 Annotation and category of the candidate genes predicted according to the subpopulation-specific significant loci 

Supplemental table 3 Sequences of the ten pairs of primers used for gene expression assay in this study


Supplementary Figure


Supplementary Figure S1

Supplementary Tables Supplemental table 1 Annotation and category of the candidate genes predicted according to the significant loci revealed by GWAS across all the testcrosses a The positions of the candidate genes were based on the public maize genome data set B73 RefGen_v2. 
b The homologs of the candidate genes in red have been reported associating with stress tolerance in Arabidopsis or rice.
Jinbo Yan, Yu Wu, Wenming Li, Xiner Qin, Yi Wang, Bing Yue
Genetic mapping with testcrossing association and F2:3 populations reveals the importance of heterosis in chilling tolerance at maize seedling stage