Transmission of human respiratory syncytial virus in the ferret model


Figure S1

. HRSV virus titer in throat and nose swabs of donor and contact ferrets; Group A: donor ferrets inoculated intra-tracheally; Group B: donor ferrets inoculated intra-nasally; Group C: immunocompromised ferrets in contact with group A ferrets; Group D: immunocompetent ferrets in contact with group A ferrets; Group E: immunocompromised ferrets in contact with group B ferrets; Group F: immunocompetent ferrets in contact with group B ferrets; each line represents a single ferret. 

Table S1. RT-PCR and sequencing primers and probes


Assay

Virus Name Sequence (5'-3') 
qPCR RSV-A RSV-A Fwd AGATCAACTTCTGTCATCCAGCAA qPCR RSV-A RSV-A Rev TTCTGCACATCATAATTAGGAGTATCAAT qPCR RSV-A RSV-A Probe Drfly-CACCATCCAACGGAGCACAGGAGAT- BHQ2 qPCR RSV-B RSV-B Fwd TTCCTAACTTCTCAAGTGTGGTCCTA qPCR RSV-B RSV-B Rev CTGGTTTCTTGGCGTACCTCTATAC qPCR RSV-B RSV-B Probe FAM-TCCCATTATGCCTAGACCTGCTGCATTG- BHQ1 Sequencing RSV-A G RT ATCTCCATCATGATTGCAATACTAAAC Sequencing RSV-A G Fw1 TGGGGCAAATGCAAACATG Sequencing RSV-A G Rev2 GTTGATTGATAAAATTCTTCAGTGATG Sequencing RSV-A G Rev 1 GCTGCATATGCTGCAGGGTAC Sequencing RSV-A G Fw 2 CACCACAAAACAACGCCAAAA Sequencing RSV-A RSV-AFcDNA AGCATATGCAGCAACAATCCAAC Sequencing RSV-A RSV-AFFw4 CAAGAACCGACAGAGGATGG Sequencing RSV-A RSV-AFOuterFw CCATGACCAAATCAAACAGAATC Sequencing RSV-A RSV-AFRev3 GCATGACACAATGGCTCCTA Sequencing RSV-A RSV-AFOuterRev AATTATGACTAAAATGACACCTCTTACC Sequencing RSV-A RSV-AFFw1 AAAATCAACTCTGGGGCAAATAAC Sequencing RSV-A RSV-AFRev 1 GTGCAGGACCTTGGATACGG Sequencing RSV-A RSV-AFFw2 GTCGAGCCAGAAGAGAACTACC Sequencing RSV-A RSV-AFRev2 AGTAACTTTGCTGTCTAACTATTTGAAC Sequencing RSV-A RSV-AFFw3 AGAACAACAGACTACTAGAGATTACCAG Sequencing RSV-A RSV-AFRev4 TGGATTTACCAGCATTTACATTATG Sequencing RSV-A RSV-AFFw5 AAAGGTGAACCAATAATAAATTTCTATG Sequencing RSV-A RSV-AFRev5 TTACCATTCAAGCAATGACCTCG

Table S2 .

 S2 Sequence mutations in the F and G genes of virus shed from donor and contact ferrets compared to the inoculum virus. 


	Ferret Group	Ferret Number	Sample type	Dpi	Ct value	F gene	G gene
	A	1	Throat swab	5	24.84		
	A	1	Nose swab	5	37.15	ND	ND
	A	1	Throat swab	11	20.07		
	A	2	Throat swab	5	18.45		
	A	2	Nose swab	5	32.30	ND	
	A	2	Throat swab	11	20.05		
	B	3	Throat swab	5	20.40	ND	
	B	3	Nose swab	5	22.51	ND	
	B	3	Throat swab	11	18.48	ND	
	B	4	Throat swab	5	23.34		
	B	4	Nose swab	5	17.90	ND	
	B	4	Throat swab	11	26.67		
	C	5	Throat swab	11	21.36		T226I
	C	6	Throat swab	11	27.01		
	C	7	Throat swab	11	22.42		Q81Q/L
	C	8	Throat swab	11	31.70	ND	
	C	9	Throat swab	11	29.56		
	D	10	Throat swab	11	19.08		
	D	11	Throat swab	11	25.04		
	D	12	Throat swab	11	23.55		
	D	13	Throat swab	11	25.32		
	D	14	Throat swab	11	33.10	ND	Partial; N20N/D
	E	15	Throat swab	11	24.40		
	E	16	Throat swab	11	24.03		
	E	17	Throat swab	11	33.58	ND	Partial
	E	18	Throat swab	11	29.40	ND	
	E	19	Throat swab	11	22.33		
	F	20	Throat swab	11	32.87	ND	Partial
	F	21	Throat swab	11	33.03	ND	Partial
	F	22	Throat swab	11	30.68	ND	
	F	23	Throat swab	11	29.50	T244T/A	
	F	24	Throat swab	11	23.91		
	Dpi: day post-inoculation; ND: no data				

Supplementary Materials:

Figure S1 Table S1 Table S2
Leon De Waal, Saskia L Smits, Edwin J B Veldhuis Kroeze, Geert Van Amerongen, Marie O Pohl, Albert D M E Osterhaus, Koert J Stittelaar
Transmission of human respiratory syncytial virus in the ferret model