The Complete Genome Sequences of 13 Species of Brachycnemina (Cnidaria, Hexacorallia, Anthozoa, Zoantharia)

Keywords: Brachycnemina, genome, Zoantharia

. Genome sequencing was performed on genetic material from single wildcollected individuals. For each species the sequence reads were assembled using a de novo method followed by a finishing step. The raw and assembled data are publicly available via GenBank. 

Introduction

We present the assembled draft genome sequences from 13 extant species of the zoantharian suborder Brachycnemina. Detailed information about the specimen used for each species can be found in the Biosample linked to each genome assembly in GenBank. 

Methods

DNA extraction was performed using the Qiagen DNAeasy genomic extraction kit using the standard process. A paired-end sequencing library was constructed using the Illumina TruSeq kit according to the manufacturer's instructions. The libraries were sequenced on an Illumina HiSeq platform in paired-end (2 × 150 bp) format. The resulting fastq files were trimmed of adapter/primer sequences and low-quality regions with Trimmomatic v0.33 (Bolger, Lohse, and Usadel 2014). The trimmed sequences were assembled by SPAdes v2.5 (Bankevich et al. 2012) followed by a finishing step using Zanfona (Kieras, O'Neill, and Pirro 2021). 

Results and Data Availability

All data, including raw reads and assembled genome sequence, are available via GenBank. 

Funding

Funding was provided by Iridian Genomes, grant# IRGEN_RG_2021-1345 Genomic Studies of Eukaryotic Taxa. Submitted: February 22, 2023 EST, Accepted: March 04, 2023 EST The Complete Genome Sequences of 13 Species of Brachycnemina (Cnidaria, Hexacorallia, Anthozoa, Zoantharia) Biodiversity Genomes 


Isaurus tuberculatus JAOEHV000000000


Palythoa caribaeorum JAOCNB000000000

Palythoa grandiflora JAOCPT000000000 

Palythoa grandis JAOEJH000000000

Palythoa heliodiscus JAOBYJ000000000 
Palythoa mutuki JAODFW000000000 

Sphenopus marsupialis JAOBRF000000000

Zoanthus gigantus JAOBBB000000000 

Zoanthus pulchellus JAOBRB000000000

Zoanthus sanctaehelenae JAOTRI000000000 

Zoanthus sansibaricus JAPEIX000000000

Zoanthus sociatus JAOBAY000000000 
Zoanthus solanderi JANCMA000000000
Maria E A Santos, Hiroki Kise, Chloé Julie Loïs Fourreau, Angelo Poliseno, Stacy Pirro, James Davis Reimer
The Complete Genome Sequences of 13 Species of Brachycnemina (Cnidaria, Hexacorallia, Anthozoa, Zoantharia)