Inhibition of HIF-1α by PX-478 enhances the anti-tumor effect of gemcitabine by inducing immunogenic cell death in pancreatic ductal adenocarcinoma


Supplementary Material




  




Supplementary Table 1. Antibodies and siRNA sequences Antibodies

  

	Name	Manufacturer	Number	Type	Usage
	HIF-1α	Abcam	ab113642	Monoclonal WB
	CRT	Cell Signaling Technology	12238	Polyclonal	WB,IF
	eIF2α	Cell Signaling Technology	9722	Polyclonal	WB
	P-eIF2α	Cell Signaling Technology	9721	Polyclonal	WB
	HMGB1	Sigma	WH0003146M8 Monoclonal WB.IF
	β-tubulin	Abmart	M30109	Monoclonal WB
	siRNAs	sequences (5'-3')			
	HIF-1α #1	CCAGCAGACUCAAAUACAATT/	UUGUAUUUGAGUCUGCUGGTT
	HIF-1α #2	UUGUAUUUGAGUCUGCUGGTT GCCUCUUUGACAAACUUAATT/	UUAAGUUUGUCAAAGAGGCTT
	HIF-1α #3	UUAAGUUUGUCAAAGAGGCTT CAGGCCACAUUCACGUAUATT/	UAUACGUGAAUGUGGCCUGTT
		UAUACGUGAAUGUGGCCUGTT			

WB: Western boltting; IF:Immunofluorescence 
Supplementary figure1: The figures of CD8

+ and CD3 + T cells isolated from peripheral blood, spleen and tumour were analyzed by flow cytometry.