Supplemental Table 1. List of oligonucleotides used in this study

  

	Oligo ID	Sequence (5' è3')	Application
	IDT3557	CCCGGATCCGATTGGAAGGATCTCAACTGGAAGCAAAAGTTTAG	pΔN--RadAi and
			pΔN--RadAi--AA
			cloning
	IDT3558	CCCGGATCCGGAAAGCATTGGAAGGATCTCAACTGGAAGC	pΔN--RadAi and
			pΔN--RadAi--AA
			cloning
	IDT3674	GAAGGGTAAGGGAGGAAAGGCGGTAGCTAGGTTAATAGAT	pΔN--RadAi R503A
			mutagenesis
	IDT3675	ATCTATTAACCTAGCTACCGCCTTTCCTCCCTTACCCTTC	pΔN--RadAi R503A
			mutagenesis
	IDT4172	CCTGCCTTGTGGCAGCTTCAAGAATTCGGTAGTGGGAAGTGCTTTGCTAGG	pXI--INT--RadA
			cloning
	IDT4173	GTAAAACGACGGCCAGTGCCAAGCTTTTATTAAGCATGGAGAACAAGTCCATTGGGAGC pXI--INT--RadA
			cloning
	Supplemental	

Table 2 . List of plasmids used in this study

 2 

	Plasmid	Comments	Source
	pΔN--RadAi	For expression of intein containing Pho	Present study
		RadA lacking residues 1--115 with an	
		amino--terminal his--tag lacking cloned	
		into BamHI/XhoI sites of pET45b(+)	
	pΔN--RadAi--AA	C153A, N324A splicing inactive mutant	Present study
		of pΔN--RadAi	
	pΔN--RadAi--R503A	R503A mutant of pΔN--RadAi	Present study
	pXI--INT--RadA	Pho RadA Intein with chitin binding	Present study
		domain; no RadA extein sequence	
	pMIG--RadAi	Pho RadA intein with short extein	Topilina et al. 2015b
		sequences cloned between MBP and	
		GFP	
	pFL--RadAi	For expression of full--length intein	Topilina et al. 2015b
		containing Pho RadA precursor with an	
		amino--terminal his--tag	
	pFL--RadAi--AA	C153A, N324A splicing inactive mutant	Topilina et al. 2015b
		of pFL--RadAi--AA	

Supplemental Materials

Protein splicing of a recombinase intein induced by single--stranded DNA and DNA damage Christopher W. Lennon, Matthew Stanger, and Marlene Belfort * *Correspondence to Marlene Belfort (mbelfort@albany.edu). This PDF includes: Supplemental Figures 1-- (C) Splicing in SOS + strains is higher than in SOS --after UV treatment. Expression of RadA in recA + /recA --and lexA + /lexA3 strain pairs was measured 2 h after UV treatment. Samples were isolated and analyzed as in panel A. 3 F´ traD36 proA + B + lacI q Δ(lacZ)M15/ Δ(lac--proAB) glnV44 e14 --gyrA96 recA1 relA1 endA1 thi hsdR17 

Supplemental


Supplemental Table

New England Biolabs