LCT

instructs the production of lactase enzyme (Rings et al., 1994) 

SLC9A3R1

encodes the Na + /H + exchanger regulatory factor 1 protein (Lin et al., 2010) ENPEP are involved in the control of sodium and water absorption, glucose uptake and absorption and digestion of peptides (Holmes et al., 2017;Penninger et al., 2021) ACE2 APOA4 
is involved between others in chylomicron assembly, cholesterol transport and blood glucose homeostasis (Kohan et al., 2015) APOA1 is a major component of the high-density lipoprotein (HDL) (Shioji et al., 2004) APOA5 is a key regulator of triglyceride levels (Garelnabi et al., 2013) HMGCS2 
encodes the rate-limiting enzyme in the production of ketone bodies(Ruiz-Roso et al., 2020) 

PLTP

transfers phospholipid and cholesterol from apo B-containing lipoproteins to HDL (Huuskonen et al., 2001) 

ME1

generates nicotinamide adenine dinucleotide phosphate (NADPH) that is used in fatty acid and cholesterol biosynthesis (Jiang et al., 2013) CYP8B1 is required for the synthesis of cholic acid (Kim et al., 2007) CLPS is a cofactor of pancreatic lipase, which allows the lipase to anchor itself to the lipid-water interface (Van Tilbeurgh et al., 1999) LIPA produces the lysosomal acid lipase (Zhang, 2018) LPL hydrolyses circulating triglycerides and releases fatty acids that can be taken up by tissues(Sylvers-Davie and Davies, 2021) 



  


Supplementary Table 3. List of antibodies

  
	1.3 Antibodies	RPMI 1640 R-Spondin Supplier	Host	Thermofisher R&D systems Dilution	11-875-093 4645-RS
	E-Cadherin	Sucrose Beckton Dickinson	Mouse	Merck 1:500	S1888
	Villin		Triton X-100 Santa cruz	Mouse	Sigma-Aldrich 1:250	10789704001
	TGR5	TrypLE™ Express Enzyme Abcam	Rabbit	Thermofisher 1:200	12604013
	GLUT2	Verapamil Santa cruz	Mouse	Sigma-Aldrich 1:200	V4629
	Phalloidin 568	Y-27632 Invitrogen		Tocris 1:500	1254/10
	Alexa Fluor 488	Invitrogen		1:500
	1.2 Supplementary Table 2. Primer sequences Alexa Fluor 647 Invitrogen	1:500
	Gene Alexa Fluor 568	5'-Forward-3' Invitrogen		1:500	5'-Reverse-3'
	ACE2 APOA1 1.4	CAAGAGCAAACGGTTGAACAC CCCTGGGATCGAGTGAAGGA	CCAGAGCCTCTCATTGTAGTCT CTGGGACACATAGTCTCTGCC
	APOA4		CTCAAGGGACGCCTTACGC	GTCCTGAGCATAGGGAGCCA
	APOA5		GCCAGCGACTTCAGGCTTT	AGCTTGCTCAGAACCTTGCC
	BCRP	ACGAACGGATTAACAGGGTCA	CTCCAGACACACCACGGAT
	CES2	CATGGCTTCCTTGTATGATGGT	CTCCAAAGTGGGCGATATTCTG
	CLDN1	CCCAGTCAATGCCAGGTACG	GGGCCTTGGTGTTGGGTAAG
	CLDN3	AACACCATTATCCGGGACTTCT	GCGGAGTAGACGACCTTGG
	CLDN5	GCAGCCCCTGTGAAGATTGA	GTCTCTGGCAAAAAGCGGTG
	CLPS	CTCTGCATGAATAGTGCCCAG	AGGGACACTTGTAGTAAATCCCA
	CYP2C9	CAGAGACGACAAGCACAACCCT	ATGTGGCTCCTGTCTTGCATGC
	CYP2J2	TGGCTTGCCCTTAATCAAAGAA	GGCCACTTGACATAATCAATCCA
	CYP3A4	AAGTCGCCTCGAAGATACACA	AAGGAGAGAACACTGCTCGTG
	CYP8B1	ATTTGGATACCGTTCAGTGCAA	CAGAAGCGAAAGAGGCTGTC
	ENPEP	CTTGACCAGATCGTGTGACTC	GGCAGTCGAAAGTTTTTCCAC
	HMGCS2	CAGTCCAAGAGGACATCAACTC	CAGTGCCTACTTCCAGCCTG
	LCT	ATCCAGACGAGAAAACAGTGC	GTCAGCAAAGGCTTCGGTTC
	LIPA		CCCACGTTTGCACTCATGTC	CCCAGTCAAAGGCTTGAAACTT
	LPL	TCATTCCCGGAGTAGCAGAGT	GGCCACAAGTTTTGGCACC
	MDR1	GGGATGGTCAGTGTTGATGGA	GCTATCGTGGTGGCAAACAATA
	ME1	GGGAGACCTTGGCTGTAATGG	TTCGGTTCCCACATCCAGAAT
	MRP1	TTACTCATTCAGCTCGTCTTGTC	CAGGGATTAGGGTCGTGGAT
	MRP2	TCTCTCGATACTCTGTGGCAC	CTGGAATCCGTAGGAGATGAAGA
	MRP3	CACCAACTCAGTCAAACGTGC	GCAAGACCATGAAAGCGACTC
	MRP4	TGTGGCTTTGAACACAGCGTA	CCAGCACACTGAACGTGATAA
	MRP5	GAACTCGACCGTTGGAATGC	TCATCCAGGATTCTGAGCTGAG
	MRP6	AGATGGTGCTTGGATTCGCC	GCCACACAGTAGGATGAATGAG
	OCLN		CATTGCCATCTTTGCCTGTG	AGCCATAACCATAGCCATAGC
	OSTA		ACCTCGTTTTATGCCGTGTG	AAGAAGGCGTATTGGAAAGGG
	OSTB	ATGGTCCTCCTGGGAAGAAGCA	GCCTCATCCAAATGCAGGACTTC
	PEPT1	GACAAGCAGTCACCTCAGTAAG	AGTCCCGAGAGCTATCAGGG
	PLTP	AAGAGCGGATGGTGTATGTGG	ATGGGGAGTCAATCACTGCTG
	SLC9A3R1	GGCTGGCAACGAAAATGAGC	TGTCGCTGTGCAGGTTGAAG
	UGT1A1	CTGTCTCTGCCCACTGTATTCT	TCTGTGAAAAGGCAATGAGCAT
	UGT1A3	TTTCACCCTGACAACCTATGC	AGCTCCACACAAGACCTATGAT
	ZO-1	CAACATACAGTGACGCTTCACA	CACTATTGACGTTTCCCCACTC

Supplementary Table 4. Functions of lipid metabolism markers Gene Function