Nucleotide Sequences of a Soybean cDNA Encoding an 18 Kilodalton Late Embryogenesis Abundant Protein'


We report the sequence of a cDNA clone, pGmPM 1, corresponding to a soybean mature seed-abundant mRNA (GmPM for Glycine max physiological mature; Table I). It was selected from a pod-dried cotyledon cDNA AZaplI library (3) screened by differential hybridization with single-stranded cDNA probes prepared from immature seeds (35 DAF) and pod-dried seeds poly(A+)2 RNA. Figure 1 shows the nucleotide sequences of the 836-bp cDNA insert and the derived amino acid sequence. 
Only one long open reading frame was found and the molecular mass of the deduced protein is similar to that predicted by hybrid select translation (3). The deduced protein is very hydrophilic, as are most other Lea proteins (2), and consists of 173 amino acid residues corresponding to a mo- lecular mass of 18 kD. Two potential poly(A+) signals, AA- TAA and AATAAA, are found in the 3'-noncoding region, 27 and 19 nucleotides, respectively, upstream from the poly(A+) tail. A search for polypeptide homologies in data- banks revealed a strong local similarity with Lea protein D1 13 from cotton (1). Recently, this gene was grouped into the LeaA gene and was demonstrated to be expressed at the late seed developmental stage as well as in mid-development seeds after excision and culture in basal medium or ABA treatment (4). 2 Abbreviations: poly(A+), polyadenylation; Lea, late embryogenesis abundant; bp, base pair. 
773 1 GAAAACCTAAGCTTAGAATTAGAAACAAAGAAAAGAAGAAAGGAAAGAATTGATCGTTGA 61 AAATCAGGGAGGAAAGAAAGCGGGAGAGTCCATTAAGGAAACAGCTACAAACATTGGTG M Q G G K K A G E S I K E T A T N I G A 121 CTTCTGCCAAGGCTGGCATGGAGAAGACCAAGGCCACTGTCCAAGAGAAGGCAGAAAGAA S A K A G M E K T K A T V Q E K A E R M 181 TGACAGCACGTGATCCGGTGCAAAAGGAGTTGGCAACCCAAAAGAAAGAGGCAAAGATGA T A R D P V Q K E L A T Q K K E A K M N 241 ACCAGGCGGAGCTGGACAAGCAGGCGGCGCGTCAGCATAACACGGCGGCCAAACAGTCCG Q A E L D K Q A A R Q H N T A A K Q S A 20 40 60 80 301 CCACCACGGCTGGGCACATGGGACATGGCCACCACACCACCGGAACCGGGACTGGAACCG T T A G H M G H G H H T T G T G T G T A 100 361 CCACGTATTCCACCACCGGGGAATATGGTCAGCCCATGGGGGCCCACCAGACGTCGGCAA T Y S T T G E Y G Q P M G A H Q T S A M 120 421 TGCCTGGCCATGGAACCGGGCAGCCCACGGGCCATGTCACCGAAGGAGTGGTGGGCTCAC P G H G T G Q P T G H V T E G V V G S H 140 481 ATCCCATTGGAACCAACAGAGGGCCGGGTGGGACCGCTACAGCTCATAATACCCGTGCGG P I G T N R G P G G T A T A H N T R A G 160 541 GTGGAAAGCCGAATGATTATGGGTATGGGACTGGGGGTAC G K P N D Y G Y G T G G T ;I'A&TTAATGATGATTATAT



  LITERATURE CITED1. Baker J, Steele C, Dure L III (1988) Sequence and characteriza- tion of 6 Lea proteins and their genes from cotton. Plant Mol Biol 11: 277-291 2. Dure L III, Crouch M, Harada J, Ho T-hD, Mundy J, Quatrano R, Thomas T, Sung ZR (1989) Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475-486 3. Hsing Y-iC, Wu S-j (1991) Cloning and characterization of cDNA clones encoding soybean seed maturation polypeptides. Bot Bull Academia Sinica 33: 191-199 4. Hughes DW, Galau GA (1991) Developmental and environmen- tal induction of Lea and LeaA mRNAs and the postabscission program during embryo culture. Plant Cell 3: 605-618 'This research was supported in part by the National Science Council to Y.C.H. and T.C. 


Figure 1 .

 1 Figure 1. Nucleotide sequence of the 836-bp cDNA insert and the derived amino acid sequence of pGmPM1 from soybean. The start codon, stop codon, and potential poly(A+) sites are indicated by underlines. 


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Zuei-Ying Chen, Yue-Ie C Hsing, Pei-Fang Lee, Teh-Yuan Chow
Nucleotide Sequences of a Soybean cDNA Encoding an 18 Kilodalton Late Embryogenesis Abundant Protein'