Nucleic Acids Research A calmodulin pseudogene on human chromosome


has been isolated from a chromosome 17 specific library. Comparison 



  of the amino acid sequence 


			hCE2	with	that	for
	three previously identified human calmodulin cDNAs,XhCE1 (1)
	hCWP (2) and xht6 (3) reveals that there are 17 amino acid
	substitutions and two amino acid deletions 83. Insertion of arg occurs between residues 41 positions 42,	63	and of
	lys and asp between residues 95 and 96. upstream region, the changes in the first	amino	the cannot 5'
	be evaluated.	These results suggest that		intronless,
	processed calmodulin pseudogene. has 43 point mutations in the putative When compared		hCE2
		Comparison of			sequence
	with that of hCWP or xht6 reveals an 85%			in
	the coding region and only a 42% nucleotide non-coding region. hCE2 is evolutionarily SC8, a rat pseudogene (4) than to hCWP		in ahCE1	3' and human
	calmodulin pseudogene, hGH6, exhibiting		acid
	substitutions has been reported (5).		
	1 GAATTCATAGMAGCTTTTTC CCTATTTGAC AGAGACAATG ATGGAACTAT AACAACAAAG 61 GAAGTGGAAA CGATAATGAG GTCTCTTGGG GTTAGAATCC AGGGTTACCA 121 GACATGATTA ATGMAGTAGA TGCTGATGGT AATA AGG 181 ATGATGGCAA GAAAAATGMA AGACACAGAC AGTGAAGAGA ATAGAGAAGC 241 TTTGATAAGG ATAAGGATGG CAATGGCTAT ATCAGTGCAG CAGAACTTTG 301 ACAAACCATG GAGAGMGCT TACAGATGMA CAGGTTGATG AMTGACTAG GGAAGCAGAT 361 ATTGATGCTG ATGGTCAAGT AACTACGA GAGTTTGTAC AGCGAAGTGA 421 AGACCTTATA CAGAATATGT TACATTTCTT GTAACAAATT 481 GTTTATAATT TATCTCTAAA AGGTTTCTTC CTACTGTCMA AAAAMACAT 541 TAGGACTCCG TTCCTCCATG TTTTCTTCTC TTATCTTACT 601 TGAAAACTTA TTTTAGAAAM TTGATCAAGT AACATGTTAC 661 ATCTMACCCT TCTGCACAGC TAAXTTAGA TGGAGTTGGT CAAAGGGGAA 721 TGTGCCTTTT TTTTTTAGTT TTCTTTAAAA ACTGCAGCAT 781 GAGTTGTAAC TCTGTACACA ATTGACAGTC GACAATATGT ACCTMAATT 841 AAAAGGGGTG ATTATCCAGG TACTCATACA CTTTTTTTAT ATATATTACT 901 CAGAAACATT TTGCTTTATT GTTACTTACT TGCTTTTTMA GGCACTTAAA 961 GAAAATCTGC TTATGGCAGA TTGCCTCAAA TCCATTCCMA 1021 TATAAAATA CMTTTACCT CCAMACCCC CAAAAGACAA AACAAAACA GMATGAAGAT 1081 ATGAAAGCTC AGGGCTCCT

an overall identity of 90%.
Sen Banani, Sevilla D Gupta, O Wesley Detera-Wadleigh1, Mcbride2
Nucleic Acids Research A calmodulin pseudogene on human chromosome