Supplementary Table

 S1-R TGGTGTTGTGGGTGAGATGA COL4A2-UTR-F1 CACAGAAAGCCAGGAGCAG 864 COL4A2-UTR-R1 TTGGGATTACAGGCGTGAG COL4A2-UTR-F2 GGGGTACCTCCAATGCCACAGACAACTC 670 COL4A2-UTR-R2 CGGGATCCAATGGCTGTAATACCACGAAT EMP1-UTR-F1 CAGGGCTGTGCATTCTTGTG 833 EMP1-UTR-R1 TCGACTGGGCTCTGACCATC EMP1-UTR-F2 GGGGTACCTTATGCGAATCGTGATGGAA 644 EMP1-UTR-R2 CCGCTCGAGAGAGGGACAATGGCTCACAG ONECUT2-UTR-F1 GTGGTGCCAGCAAACTTCTTA 1266 ONECUT2-UTR-R1 TATACCCTGTTGACCGTCCCT ONECUT2-UTR-F2 GGGGTACCGCCAAAGAGCATCATAGGAAA 756 ONECUT2-UTR-R2 CCGCTCGAGGCAATAATACCCGTGAAAGCA SNX1-UTR-F1 TTCCCTCTTTATCTCCCAGTC 1101 SNX1-UTR-R1 GAGCACCAGTCTTACCAACC SNX1-UTR-F2 GGGGTACCGGTTAATGCTGGGCTTGGTA 721 SNX1-UTR-R2 CCGCTCGAGAGAGGCTGAAGAGGACGACA SNX1-ORF-F1 AGTTGTTGTTGCGGCTTCC 1924 SNX1-ORF-R1 CCAGTTCCTAAACCACATCCTG SNX1-ORF-F2 GAAGATCTATGGCGTCGGGTGGTGGT 1584 SNX1-ORF-R2 GGAATTCTTAGGAGATGGCCTTTGCCT UBE4B-UTR-F1 TCTGCTAGACACAGCCAAGG 1003 UBE4B-UTR-R1 TGTTGAAACCGGCAGACATA UBE4B-UTR-F2
GGGGTACCAGCAAACGCTGAGACCTGA 856 UBE4B-UTR-R2 CCGCTCGAGTGCACCCTCTTTTGCTTTG *: Sequences underlined are sites for restriction enzymes. 
Supplementary Table S3 

. Patients' information

  
		Colorectal cancer	Colorectal cancer*
	Characteristics	(n=146)	(n=87)
	Gender		
	Male	82	50
	Female	64	37
	Age at diagnosis		
	Mean ± SD	58±11	60±11
	Median(range)	58(28-80)	59(31-80)
	TNM stage		
	I	19	13
	II	46	26
	III	62	41
	IV	19	7
	Nodal status		
	Positive	79	48
	Negative	67	39
	T stage		
	T1	3	3
	T2	22	14
	T3	36	21
	T4	85	49
	Tumor location		
	Rectum	62	39
	Distal colon	38	24
	Proximal colon	46	24
	Histology		
	Adenocarcinoma	139	84
	Mucious adenocarcioma	5	3
	Signet-ring cell carcinoma	2	0
	*: Of these 146 cases, matched frozen noncancerous tissues were available in 87 cases.

Supplementary Table S2. Sequences of PCR primers

  
	Primers	Sequence (5 'to 3') *	Products length
	pri-miR-95-F	TTGCCTTGTTTCCCGATTT	605
	pri-miR-95		

. Significant dysregulation of miRNAs in primary CRC

  T: tumor tissue; NCT: noncancerous tissue § :P-values are calculated on log-signal of microRNA expression measured by microarray. 


		hsa-miR-145	0.38	0.00219
		hsa-miR-133b	0.36	0.00176
		hsa-miR-375	0.35	0.00722
		hsa-miR-513c	0.33	0.00858
		miRNA Name hsa-miR-650 hsa-miR-381	Fold change(T/NCT) † 0.28 0.25	0.00784 P value § 0.00442
		hsa-miR-135b hsa-miR-378*	16.29 0.25	0.00001 0.00067
		hsa-miR-31 hsa-miR-551b	15.45 0.21	0.00121 0.00063
		hsa-miR-183 hsa-miR-9	10.84 0.13	6.53E-07 0.00003
		hsa-miR-182 hsa-miR-30a*	6.88 0.11	0.00023 0.00032
		hsa-miR-421 hsa-miR-139-5p	5.20 0.10	0.00016 0.00010
		hsa-miR-224 hsa-miR-204	4.88 0.08	0.00005 9.84E-08
	†	hsa-miR-431*	4.13	0.00992
		hsa-miR-502-5p	3.86	0.00382
		hsa-miR-18b	3.77	0.00042
		hsa-miR-424	3.60	0.00006
		hsa-miR-18a	3.51	0.00086
		hsa-miR-96	3.38	0.00080
		hsa-miR-1201	3.06	0.00945
		hsa-miR-769-5p	2.43	0.00869
		hsa-miR-301a	2.38	1.10E-06
		hsa-miR-335*	2.37	0.00581
		hsa-miR-223	2.31	0.00011
		hsa-miR-95	2.29	0.00518
		hsa-miR-7	2.28	0.00587
		hsa-miR-21*	2.26	0.00013
		hsa-miR-17	2.20	0.00164
		hsa-miR-221	2.10	0.00045
		hsa-miR-1274a	2.06	0.00350
		hsa-miR-20b	2.01	0.00296
		hsa-miR-20a	2.01	0.00151
		hsa-miR-19a	2.01	0.00325
		hsa-miR-29c*	0.49	0.00230
		hsa-miR-145*	0.47	0.00334
		hsa-miR-1826	0.47	0.00508
		hsa-miR-638	0.45	0.00562
		hsa-miR-497	0.45	0.00093
		hsa-miR-572	0.43	0.00227
		hsa-miR-195	0.42	0.00033
		hsa-miR-326	0.41	0.00180
		hsa-miR-338-3p	0.40	0.00054
		hsa-miR-378	0.40	0.00060
		hsa-miR-1	0.38	0.00863

: 
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