Supplementary Table

. Body weight changes in the experiment on the effect of CNP on food intake in refeeding following 48-hr fasting. 
Data represent mean ± SEM. Significant difference: ** p < 0.01. 

Saline

(13) 1.8 ± 0.2 CNP-22 0.5 nmol (8) 1.0 ± 0.3 1.5 nmol (13) 1.2 ± 0. 

Supplementary Table 1. PCR Primers

mRNA Sense: 5'-CCCAACGTTGCTGAGAACGAGTCG-3' Antisense: 5'-GGAGGTCATGAAGCCACCGTAACG-3' Primers POMC CART NPY AgRP Sense: 5'-AGAGTAAACGCATTCCGATCTACGA-3' Antisense: 5'-TCCTCACTGCGCACTGCTCT-3' Sense: 5'-CTGACCCTCGCTCTATCTCTG-3' Antisense: 5'-AGTATCTGGCCATGTCCTCTG-3' Sense: 5'-TTGTGTTCTGCTGTTGGCACT-3' Antisense: 5'-AGCAAAAGGCATTGAAGAAGC-3' 

GAPDH

Sense: 5'-TGCACCACCAACTGCTTAGC-3' Antisense: 5'-GGATGCAGGCATGATGTTCTG-3' 


Supplementary Table

 3. Body weight changes in the experiment on the effect of CNP on nocturnal food intake. 

Data represent mean ± SEM.

Supplementary Figure 1. Effects of icv administration of CNP-53 (1.5 nmol/mouse) on mRNA expressions of (A) POMC, (B) CART, (C) NPY and (D) AgRP in the hypothalamus. The hypothalamus was collected at the end of the experiment to examine the effect of CNP-53 on food intake in refeeding following 48-hr fasting. Data represent mean ± SEM. The number of mice is given in parentheses. Significant differences: * p < 0.05, ** p < 0.01. 

Saline