Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression


Supplementary Materials

Supplementary Table S1 



  




  




  


: Primer list qRT-PCR

  
	GAPDH	forward	GGAGCGAGATCCCTCCAAAAT
		reverse	GGCTGTTGTCATACTTCTCATGG
	U6	forward	GTGCTCGCTTCGGCAGCACA
		reverse	GCAAATTCGTGAAGCGTTCC
	COTL1	forward	AGTTGACGGAGGGAGTATTT
		reverse	CAGACGCAGGACTGAAGC
	GATA3	forward	GCCCCTCATTAAGCCCAAG
		reverse	TTGTGGTGGTCTGACAGTTCG
	UCA1	forward	TTTATGCTTGAGCCTTGA
		reverse	CTTGCCTGAAATACTTGC
	ChIP-qPCR		
	GATA3	forward	AAGAATGTGATAGCAACG
		reverse	AGCTCCAAACAATGACAG

Supplementary Figure S1:

(A) qPCR showed miR-506-3p expression was downregulated in A549, HCC827 and SPC-A1 cells compared with that of BEAS-2B cells. (B) qPCR showed miR-506-3p were upregulated when transfected by different miR-506-3p mimics in A549 cells. #1 miR-506-3p was selected in the present study. C. qPCR showed miR-506-3p were downregulated when transfected by different miR-506-3p siRNAs in HCC827 cells. siRNA-miR-506-3p #1 was selected in the present study. Asterisk (*) indicated P < 0.05. 

Supplementary Figure S2: