CmMYB2

Forward AGAAGCCGTTTGTGCCGTTTAG Reversed CCGTTACACATTCCGTTACCA 

CmMYB3

Forward TCATCTAGCACGGTTTCGTATG 
Reversed TGGCACACTTGTATTTGTACACGT 

CmMYB4

Forward TGGACTATAGAAGCTTGGAGATGA Reversed GCTTGCATACGACTATACGAGTGA 

CmMYB5

Forward GCGTTGCGTCAACATCTAGTG 

Reversed CAACACACGAACTCCTTCTTC


CmMYB6

Forward ACGGCGAAATAGGGTGGTCATTAG 

Reversed GGATTGCAATATCATAGTTGGTCCG


CmMYB74

Forward ACATTTCCTCGTCACCAACATCT 

Reversed GAAACTCAAACTGCTTGTCTGTGGT

CmMYB#1 Forward GAGTCTCCAGACAATTCAGATC Reversed GAACAACCACACCACCAACTG CmMYB#2 Forward CTCATACTCGCCAAACTCGTC Reversed GAGTAGACAACCTGTCAAGTAG CmMYB#3 Forward CCAGGAATTGGCCACCATTG Reversed CCTGAATGCTTTAGCTAAGTCC CmMYB#4 Forward GCGAGGTAGATGACATTGTGG Reversed CTCAGGTAACTCGGGTAGTTCC CmMYB#5 Forward CATTGGGAGCAGGGATCCCGAGC Reversed CTCTGGGTGGGTTTTGCTCTC CmMYB#7 Forward TACAGGATGCACAAGCTTGTTG Reversed ACATCGTATGAGACAAAGTGTC CmMYB#8 Forward TCGTGCTACCGTACTTGCCTG Reversed GCCACAACTCATCCATGTTCC CmMYB#9 Forward CAATACATCTCCACCGTACAG Reversed GATGCACTTAAAGACACTTTGG CmMYB#10 Forward CATGGATGATGACATGGTTTTGG Reversed GTGGTAATCCATCTCCTCTTGTG CmMYB#11 Forward GATCATTCAGGGTCGAAAATGAG Reversed CATATCTGATGGAAGACATCAG CmMYB#12 Forward CGTTAGGGCTTCCTGGGATGG Reversed CCTTGATTATCACAATACGATG CmMYB#13 Forward CCCACTGACTCTCCGCTACCTCC Reversed GCATTACTAATAGCAACTTCACC CmMYB#14 Forward GCTGGGACAAGATGATGAGTGG Reversed GGCCCCGTCGAGTTGGCTTGCGG CmMYB#15 Forward GTGATGGTCTATTAGCGGATCC Reversed CATCACCATCAAAATTCACCTTC CmMYB#16 Forward GCATGAGCTCAAAATCTTCACAG Reversed AACCTTAATTCCATTGCCAATG CmMYB#17 Forward GGATAAGCAGTGGTGACATGGC Reversed CCAAGTGTCTATCTAGCTACATG CmMYB#18 Forward CGTTGCAACTTTACTAGAGGTG Reversed CTCTAAAGCATGAGAGAACTAG CmMYB#19 Forward CTATGGCACAAGGAATTCCGAGC Reversed TTGCAAGGTGGTGACTGAGAAG CmMYB#20 Forward CAATGTGGGTGTTGTGGAGGAGG Reversed CTCCCTAACTTCTCTAGCAATC CmMYB#21 Forward GGATGTTTCGCAATCAGTTTCG Reversed CACTTTGTGTTGCAGCCTCATC CmMYB#22 Forward GGAGGAAGCAGAAGAGATAACAC Reversed CATATTCCAAATATTCACCATCG CmMYB#23 Forward ATGGGGCCTAAACCAGAACCTG Reversed GTAGTGCGGTATCAGATGAGTC CmMYB#24 Forward CCAGAAGATGGTGACAGTAGCG Reversed CATCTCCATCTACTCGACAAG CmMYB#25 Forward GTGTCGCGAGATGTAGCGACAG Reversed CCTCTTCACCACAGGCACCACC CmMYB#26 Forward CGCTTCCTGGAGCGGAGTCGAG Reversed CCTCACTTCCATTCTAATCATC 




  Fig. S1. HPLC analysis of extracts of 'Turning red Jimba' (TRJ) and white 'Jimba' (WJ) 'Jimba' petals. Y-axis indicates the absorbance at 530 nm. Three peaks were detected: cyanidin-3-glucoside (1), cyanidin-3-O-(6-O-acetyl-β-D-glucoside) (2), cyanidin 3-O-(6''-O-succinyl-β-glucopyranoside) (3). 


Fig. S2 .

 S2 Fig. S2. The expression patterns of anthocyanin biosynthetic genes and two reported transcription factors were studied in white 'Jimba' (WJ) and 'Turning red Jimba' (TRJ). Error bars are the S.E. of three independent experiments. 


Fig. S4 .

 S4 Fig. S4. Phylogenetic analysis of 7 CmMYBs and reported MYBs related to phenylpropanoid pathway in other plant species. Neighbor-joining Tree was constructed using MEGA7. MYBs from chrysanthemum were marked with black solid circle. 


Fig

  Fig. S5 Regulatory effects of transiently over expressing CmMYB#85, CmMYB#15, CmMYB#16, CmMYB#17 with CmMYB6 or CmbHLH2 on transcription from CmDFR and CmUFGT promoters. SK refers to 'Empty vector'. Error bars are the S.E. of three independent experiments. 


Fig

  Fig. S6 The expression patterns of CmMYB#7 in different type petals of white 'Jimba' and 'Turning red Jimba' were measured by real-time PCR. Error bars are the S.E. of three independent experiments.