Table S1. Oligonucleotides used in this study


Primers

Sequences (5'-3') 

For amplification of CDS


HcSMA2-F ATGAGCATACAAACGAAG


HcSMA2-R ATGAGCATACAAACGAAG

For the preparation of dsRNA 
Hcsma2i-F1 TAATACGACTCACTATAGGGAGACATTGGGCGACAATCAGCTA Hcsma2i-R1 AAGCTTATGGATGAGATCGGTCGAGG Hcsma2i-F2 AAGCTTCATTGGGCGACAATCAGCTA Hcsma2i-R2 TAATACGACTCACTATAGGGAGAATGGATGAGATCGGTCGAGG Btcry1Ac-F1 TAATACGACTCACTATAGGGCCAATACAGTACCAGCTACAG Btcry1Ac-R1 GGATCCGATTCGGCTCTCCACAC Btcry1Ac-F2 GGATCCCCAATACAGTACCAGCTACAG Btcry1Ac-R2 AATACGACTCACTATAGGGGATTCGGCTCTCCACAC For real-time PCR rtHcsma2-F ATCCCACCAGGAATTCGACG rtHcsma2-R CATCGTCCACGTGCATTTGA Tubulin-F TGTTCCATCACCCAAGGTATCC Tubulin-R TGACAGACACAAGGTGGTTGAGAT 18S-F AATGGTTAAGAGGGACAATTCG 18S-R CTTGGCAAATGCTTTCGC

F.For

  Li et al. (2020) SupplementalsHcSMA2 is involved in the larval development of H. contortus represent restriction sites, and those in bold letters indicate the T7 promoter site. 


Table S2 . Sequences of siRNA used for RNA interference (5'-3')

 S2 
				Position of the
	siRNAs	Sense strand	Antisense strand
				target sequence
	siRNA-136 GCCAGUCUGGAAUUCGCAUTT	AUGCGAAUUCCAGACUGGCTT	136-154
	siRNA-625 CCAGAACAUUGGGCGACAATT	UUGUCGCCCAAUGUUCUGGTT	625-644
	siRNA-1130 GCUGGAUCGAAAUUCAUUUTT	AAAUGAAUUUCGAUCCAGCTT	1130-1149
	Negative siRNA UUCUCCGAACGUGUCACGUTT	ACGUGACACGUUCGGAGAATT	/