Supplementary Tables S1-S2 and Figure Legends Supplementary Table S1. Patients' information


RAB22A-R AGACACCATGCAATCACCAACAGC β-actin-F AGTGTGACGTGGACATCCGCAAAG 220 β-actin-R ATCCACATCTGCTGGAAGGTGGAC TRPM3-F CCAAGCTCAGCATCTGTTCA 93 TRPM3-R TCCTGGTGTCCTTCTGATCC 3'UTR primer RAB22A-Out-F ACTACGCCGACTCTATTCA 983 RAB22A-Out-R ACCTTCTTCACTTCCACTCT RAB22A-In-F CGGAATTCAAGACAGCCTTCAGAGCCA 669 RAB22A-In-R CGGGATCCGAAGCAAACCCAGGATGAG BCL2-Out-F TTTGAGTTCGGTGGGGTCAT 589 BCL2-Out-R AGCCTCCAGCAGCCAGAAAG BCL2-In-F CGGAATTCATGCCTGCCCCAAACAAATA 412 BCL2-In-R CGGGATCCTTCCATCCGTCTGCTCTTCA KLF12-Out-F GTTTAGCTCATTATACGTGGTG 758 KLF12-Out-R TAACCTTCATGCCTTCATTC KLF12-In-F CGGAATTCGGAGTTAAGAGTGCCCAGAG 465 KLF12-In-R CGGGATCCAGCAATGCACCAACAGAATA CCPG1-Out-F CCAGTGAAGTAGTGACGGAA 860 CCPG1-Out-R GCTAAACTACAGGAAAATGC CCPG1-in-F CGGAATTCGCGGTGTAGGTGTTGTGAGC 578 

CCPG1

  -Out-R GCAAGGTCAAAAGCCAACGA RAB22A-ORF-In-F ATAGGATCCCGGGCCATGGCGCTGAG 631 RAB22A-ORF-In-R TCATCACTAGTTCTGAGAGGCTGAGGTTCGG 


Supplementary Table S2. Primer sequences

  
	Primers	Sequence (5 'to 3')		Products
				length
	Primers for pri-miR-204 cloning		
	pri-miR-204-OUT-F	CTCCCTATTATTTATCATCC		
		AGTTGCCTACAGTATTCAC		
	pri-miR-204-F	CGCGGATCCCATTTACCCACAGGACAG	
		CCGGAATTCGGTTTGGACCCAGAACTA	
	Primers for pre-mir-204 copy number variation		
	pre-mir-204-CNV-F	CCAGAGCTGCTTGGATGAAGT		89
	pre-mir-638-CNV-R	TGTGACTCGTGGACTTCCCT		
	LINE1-CNV-F	TGCTTTGAATGCGTCCCAGAG		124
	LINE1-CNV-R	AAAGCCGCTCAACTACATGG		
	TNM stage Real time PCR Primers		
	I RAB22A-F	19 TACCAAAGAGGCAAAGCATGTGCG 12	124
	II	42	22	
	III	59	39	
	IV	20	7	
	Nodal status			
	Positive	63	34	
	Negative	77	46	
	Tumor size (cm)			
	<5	84	46	
	≥5	56	34	
	Tumor location			
	Rectum	60	37	
	Distal colon	34	20	
	Proximal colon	46	23	
	Grading			
	G1	6	5	
	G2	108	61	101
	G3	26	14	
	*: Both the miR-204-5p and RAB22A expression data were available in 140 cases.
	# :Of these 140 cases, matched frozen noncancerous tissues were available in 80 cases.
	$: Test cohort of CRC for RAN22A analysis.		

A,The most effective siRNA (siRNA-1) was selected out for further investigation. B, the protein levels of RAB22A were determined by western blotting in LoVo and HCT116 cells transfected with miR-204-5p mimic, RAB22A, siRAB22A or control. Beta-actin served as an internal control.
Supplementary Tables S1-S2 and Figure Legends Supplementary Table S1. Patients' information