Figure S3. Fitting procedure to obtain k n and k 2 . We fitted the unseeded aggregation data, only using the experimental data points from 0.15 to 0.72 in the normalised relative fibril mass (y-axis). The k + values were obtained from the seeded kinetics aggregation assays (Figure S1). Red dots represent the experimental data for each replicate and blue lines represent the fitting results. From top to bottom, each row represents one antibody: DesAb-HETLTLR, DesAb-FETLTLR, DesAb-AETLTLR, DesAb-HETLNLV, DesAb-FETLNLV, DesAb-RVIAHQELK, DesAb-IAHQELK, DesAb-FAHQELT and DesAb-FAHQELK. In each row, from left to right: DesAb:Aβ42 ratios 1:2(triplicate), DesAb:Aβ42 ratios 1:4(triplicate) and DesAb:Aβ42 ratios 1:8(triplicate). 
M E V Q L V E S G G G L V Q P G G S L R L S C A A S G F N I 30 K D T Y I G W V R R A P G K G E E W V A S I Y P T N G Y T R 60 Y A D S V K G R F T I S A D T S K N T A Y L Q M N S L R A E 90 D T A V Y Y C A A G S Designed CDR3 E E E A A A W G Q G T L 120 V T V S S G S H H H H H H H

Figure S2 .

 S2 Figure S2. Fitting procedure to obtain k + . We fitted the seeded aggregation data by using the equation r = 2k + P 0 m from Ref. 16 . Red dots represent the original data and blue lines represent the fitting results. From top to bottom, each row represents one antibody: DesAb-HETLTLR, DesAb-FETLTLR, DesAb-AETLTLR, DesAb-HETLNLV, DesAb-FETLNLV, DesAb-RVIAHQELK, DesAb-IAHQELK, DesAb-FAHQELT and DesAb-FAHQELK. In each row, from left to right: DesAb:Aβ42 ratios 1:2(triplicate), DesAb:Aβ42 ratios 1:4(triplicate) and DesAb:Aβ42 ratios 1:8(triplicate). 


Figure S4 .

 S4 Figure S4. Amino acid sequence of the DesAb scaffold used in this work. The sequences of the various designed CDR3 employed are in Fig. 1. 


Figure S5 .

 S5 Figure S5. Time dependence of the reactive flux toward oligomers at 1:2 DesAb:Aβ42 ratio. A 1 μM Aβ42 solution in the presence of either buffer (green) alone or with 0.5 μM (red) of DesAb; results are averaged over three replicas. 


Figure S6 .

 S6 Figure S6. Time dependence of the reactive flux toward oligomers at 1:4 DesAb:Aβ42 ratio. A 1 μM Aβ42 solution in the presence of either buffer (green) alone or with 0.25 μM (red) of DesAb; results are averaged over three replicas. 


Figure S7 .

 S7 Figure S7. Time dependence of the reactive flux toward oligomers at 1:8 DesAb:Aβ42 ratio. A 1 μM Aβ42 solution in the presence of either buffer (green) alone or with 0.125 μM (red) of DesAb; results are averaged over three replicas. 




  




  


Table S1 .

 S1 Oligonucleotide primer sequences of the DesAbs used in this work.These sequences of primers are based on the scaffold of DesAb-HETLTLR from Ref.16. 


	DesAb-HETLNLV
	Fwd GAAACCCTGaacTTAgtgGAAGAGGAAGCGGCAGC
	Rev atgCGATCCTGCTGCGGAAACCCTGaacTTAg
	DesAb-FETLTLR
	Fwd AGGATCGtttGAAACCCTGACCTTACGCGAAGA
	Rev GTTTCaaaCGATCCTGCTGCGCAATAGTACACC
	DesAb-AETLTLR
	Fwd AGGATCGgcgGAAACCCTGACCTTACGCG
	Rev GCTGCGCAATAGTACACCAGGATCGgcgG
	DesAb-FETLNLV
	Fwd AGCAGGATCGtttGAAACCCTGaacTTAgtgGAAGAGGAAGCGGCAGC
	Rev CaaaCGATCCTGCTGCGCAATAGTACACCGC
	DesAb-RVIAHQELK
	Fwd ATCGcgcgtgattgcgcatcaggaactgaaaGAAGAGGAAGCGGCAGC
	Rev tcacgcgCGATCCTGCTGCGCAATAGTAC
	DesAb-IAHQELK
	Fwd GGATCGattgcgcatcaggaactgaaaGAAGAGGAAGCGGCAGC
	Rev gcgcaatCGATCCTGCTGCGCAATAGTACACC
	DesAb-FAHQELT
	Fwd TCGTTTgcgcatcaggaactgaccGAAGAGGAAGCGGCAGC
	Rev gatgcgcAAACGATCCTGCTGCGCAATAGTAC
	DesAb-FAHQELK
	Fwd GGATCGTTTgcgcatcaggaactgaaaGAAGAGGAAGCGGCAGC
	Rev gcgcAAACGATCCTGCTGCGCAATAGTACACC

Supporting information

Electronic Supplementary Material (ESI) for Chemical Science. This journal is © The Royal Society of Chemistry 2021