Activation of protein kinase G after repeated cocaine administration is necessary for the phosphorylation of α-amino-3-hydroxy-5-methyl-4isoxazolepropionic acid receptor GluA1 at serine 831 in the rat nucleus accumbens


Supplementary Table

 S1. Rates of changes in the immunoreactivity of pGluA1-S831 in the NAc after repeated systemic injections of saline or cocaine at different time points 

Table S2 .

 S2 Rates of changes in the immunoreactivity of pGluA1-S831 in the NAc after intra-NAc infusion of KT5823 or Rp-cGMP prior to the final injection of saline or cocaine 


	Supplementary		
			Repeated saline	Repeated cocaine
			Repeated saline	Repeated cocaine
		Treatment	Mean ± SEM
	Time (min)	Vehicle	Mean ± SEM 100 ± 14.02	260.8 ± 22.053 *
	5 WB	KT5823	100 ± 15.412 114.225 ± 3.383	127.33 ± 39.443 123.575 ± 14.446 #
	10	Rp-cGMP	100 ± 12.428 113.2 ± 2.587	129.873 ± 6.131 88.3 ± 14.849 #
	30	Vehicle	100 ± 16.493 100 ± 3.095	422.652 ± 52.047 * 193.1 ± 11.656 *
	60 IHC	KT5823	100 ± 23.862 111.525 ± 4.509	264.855 ± 24.21 * 111.025 ± 8.742 #
	120	Rp-cGMP	100 ± 4.517 107.025 ± 6.716	164.846 ± 15.488 # 113.225 ± 7.035 #
	240		100 ± 5.722	121.777 ± 3.903 #

*p < 0.05 vs. repeated saline group at the same time point; # p < 0.05 vs. repeated cocaine group at the 3 Supplementary *p < 0.05 vs. repeated saline vehicle group; # p < 0.05 vs. repeated cocaine vehicle group; WB, western immunoblotting; IHC, immunohistochemistry. 
Table S4 .

 S4 Rates of changes in the immunoreactivity of PKG, pGluA1-S831 or GluA1 in the GluA1 precipitate of the NAc after the repeated systemic injections of saline or cocaine 


		Repeated saline	Repeated cocaine
	Treatment	Mean ± SEM	
	PKG	100 ± 5.289	228.4 ± 13.16 *
	pGluA1 S831	100 ± 11.53	202 ± 9.809 *
	GluA1	100 ± 8.264	106.7 ± 8.209

*p < 0.05 vs. repeated saline group. 
Supplementary Table

 S3. Rates of changes in number of co-localization of pGluA1-S831 with NeuN in the NAc after the intra-NAc infusion of KT5823 or Rp-cGMP prior to the final injection of saline or cocaine 

Repeated saline

Repeated cocaine
Ju Hwan, Su Yeon, Jeong Hwan Oh, In Soo Ryu, Jieun Kim, Dong Kun Lee, Yeonhee Ryu, Eun Sang Choe
Activation of protein kinase G after repeated cocaine administration is necessary for the phosphorylation of α-amino-3-hydroxy-5-methyl-4isoxazolepropionic acid receptor GluA1 at serine 831 in the rat nucleus accumbens