CsrA coordinates compatible solute synthesis in Acinetobacter baumannii and facilitates growth in human urine


2


SUPPLEMENTAL MATERIAL

0.45 g). The control groups were injected with or 10 µl saline () or were not injected at all (). 

29

Caterpillars were incubated in the dark over 5 day at 37 °C and the number of survived animals 30 was determined. Caterpillars were considered dead if they did not respond towards gentle poking. 

31

Error bars denote the standard deviation calculated from at least three biological replicates. 

Fig. S1

 S1 Fig. S1 Galleria mellonella killing. A. baumannii ATCC 19606 () and ∆csrA () were grown 


Fig.

  Fig. Desiccation resistance of A. baumannii ATCC 19606 and ∆csrA. An overnight culture of 


36Fig.

  Fig. Growth of A. baumannii ATCC 19606 and ∆csrA mutant in mineral medium with 200 mM 




  


Table S1

 S1 Primers used in this study. 


	22	
	csrA_upstream fw	CCCGGGGGATCCACTAGTTCCGGTACTTCTATGGGTAC
	csrA_upstream rev	CAACTTCATAGTTTATCTCCTTGCTAAACG
	crsA_downstream fw	GGAGATAAACTATGAAGTTGTTCTCTCCC
	crsA_downstream rev	CCGCGGTGGCGGCCGCTCTAGATATTCTTATGTAGTGTAATGAC
	pBIISK_sacB_kan R fw	TAGAGCGGCCGCCACCGC
	pBIISK_sacB_kan R rev	GAACTAGTGGATCCCCCGGGC
	csrA_up fw	AGAATTTGACGTCGAATTCGCACGTGAAGTTTCAACAC
	csrA_up rev	CCTGAGGCCTGCAGCGGCCGCTAACGATTGAAGTTTTTCTG
	pBAV1k fw	CGGCCGCTGCAGGCCTCA
	pBAV1k rev	CGAATTCGACGTCAAATTCTATCATAATTGTGGTTTCAAAATCGGCTC
	23	
	24	

am Main, Max-von-Laue-Str. 9,
Josephine Joy Hubloher, Kim Schabacker, Volker Müller, Beate Averhoff
CsrA coordinates compatible solute synthesis in Acinetobacter baumannii and facilitates growth in human urine