Oligos used for PCR amplification of the ZRS and sZRS enhancer from different species. Also listed are primers used for the 4C-seq analyses, genomic deletion target sites (PAM sequence in bold), genomic deletions screening and lamprey cloning. Organism ZRS Forward (5') Primer ZRS Reverse (3') Primer M. musculus AGTTCAAGAAACCGCACACC GACAGCAACATCCTGACCAA A. carolinensis GGCAGAGACAGACGTGATGA TGGCATGCTTTTCCTAGCTT L. menadoensis CAGCAAAGACCCCCAAATAG GGAAAAAGACATTTTTAGTGTCTCC O. latipes ACTTTCTTCTGGTGTCGGTC TCACCGCAGAAAGCTCATTA D. rerio