Figure S1. IRF1 exhibited similar effects in a Chinese cohort of patients with CRC. (A) Kaplan Meier survival plots of the association between overall survival time and IRF1 expression in GSE71187 datasets. The association between IRF1 mRNA expression and the ratio of (B) M1 macrophages and activated (C) CD4 + memory T cells based on GSE71187 datasets. The histogram exhibits the frequency in the value. The violet dotted line represents the median value of log 2 (IRF1 expression) and the green dotted line indicates the median value of the ratio of (B) M1 macrophages or (C) activated CD4 + memory T cells. The blue line indicated the fit line of the association between the log 2 (IRF1 expression) and cell ratio of (B) M1 macrophages or (C) activated CD4 + memory T cells. IRF1, interferon regulatory factor 1; CRC, colorectal cancer. 




  


Table SI .

 SI Hub gene identification in the yellow module by a sum rank.Co-expression degree indicates the number of connections that the indicated gene has to other genes in the co-expression network of the yellow module; PPI degree indicates the number of edges the indicated gene has to other genes in the yellow module; N co-expression rank and N ppi rank indicate the rank of the gene based on its rank of co-expression degree and PPI degree, respectively, among all genes listed in the yellow module. N sum rank =N ppi rank +N co-expression rank. PPI, protein-protein interaction. 


	Table SI. Continued. Table SI. Continued.					
	Gene symbol Gene symbol Gene symbol	Co-expression degree Co-expression degree Co-expression degree	PPI degree PPI degree PPI degree	Co-expression rank Co-expression rank Co-expression rank	PPI rank PPI rank PPI rank	Sum rank Sum rank Sum rank
	IRF1 LAMP3 RP11-326I11.5	110 99 8	39 1 0	1 25 118	2 86 119	3 111 237
	CCL5 NMI CTA-384D8.35	108 74 0	27 12 0	2 74 120	9 40 120	11 114 240
	UBE2L6 SLAMF7	107 85	24 6	4 55	13 59	17 114
	GBP1 APOL6	100 98	33 1	16 27	5 87	21 114
	IL2RB RNF213	104 66	22 14	8 80	20 36	28 116
	STAT1 BATF2	97 86	44 5	28 51	1 65	29 116
	IFIT3 FBXO6	97 89	31 4	29 48	6 70	35 118
	PSMB9 ICOS	102 75	21 8	12 72	23 51	35 123
	SAMD9L ETV7	103 97	20 0	10 31	25 97	35 128
	GBP4 ZBP1	108 54	15 12	3 89	32 41	35 130
	GBP5 MICB	100 86	23 2	17 52	19 79	36 131
	CXCL10 USP30-AS1	95 96	37 0	35 34	3 98	38 132
	PARP9 EPSTI1	100 52	21 11	18 91	24 42	42 133
	IDO1 TNFRSF14	102 76	17 5	13 69	29 66	42 135
	IFI35 RP1-93H18.6	93 95	28 0	39 37	8 99	47 136
	CD2 TNFRSF18	99 59	18 7	22 85	27 56	49 141
	OAS2 IKZF3	92 83	27 2	41 61	10 80	51 141
	GZMA TRBC1	99 92	15 0	23 42	33 100	56 142
	PSMB10 IFITM1	100 20	13 17	19 113	37 30	56 143
	PSME1 SIRPG	104 84	8 1	9 58	48 88	57 146
	PSMB8 BTN3A2	89 83	24 1	45 62	14 89	59 151
	NLRC5 LGALS9	100 43	10 7	20 99	43 57	63 156
	TAP1 LYN	103 45	7 6	11 97	52 60	63 157
	WARS MMP12	107 60	6 3	5 84	58 76	63 160
	CXCL9 IL18R1	91 50	22 4	43 92	21 71	64 163
	USP18 MAX	86 65	24 2	50 82	15 81	65 163
	GNLY ERAP1	102 34	7 6	14 103	53 61	67 164
	CD247 LYSMD2	99 73	10 1	24 75	44 90	68 165
	APOL3 RFX5	105 69	5 1	6 76	62 91	68 167
	IFIH1 APOL2	80 79	36 0	65 66	4 101	69 167
	DHX58 LAX1	83 58	24 2	59 86	16 82	75 168
	TRIM21 CCDC109B	85 77	22 0	53 68	22 102	75 170
	CTLA4 HSH2D	75 54	31 2	70 90	7 83	77 173
	RTP4 MLKL	83 38	24 4	60 102	17 72	77 174
	PARP14 IGFLR1	89 75	16 0	46 73	31 103	77 176
	IL15RA GSDMD	101 50	5 2	15 93	63 84	78 177
	RARRES3 TAPBPL	105 19	3 5	7 115	73 67	80 182
	CXCL13 TFAP2A-AS1	96 67	8 0	32 79	49 104	81 183
	CXCL11 SLC25A28	85 66	18 0	54 81	28 105	82 186
	CD3D GFI1	93 23	9 3	40 110	47 77	87 187
	CXCR6 RP11-468E2.5	88 63	10 0	49 83	45 106	94 189
	OAS3 SOD2	68 43	24 1	77 100	18 92	95 192
	CMPK2 C18orf8	82 56	15 0	63 87	34 107	97 194
	LAP3 ICAM3	96 17	5 3	33 117	64 78	97 195
	BIRC3 PLA2G2D	91 55	7 0	44 88	54 108	98 196
	TAP2 JADE2	97 33	4 1	30 104	68 93	98 197
	APOBEC3G FAM46C	98 29	3 1	26 106	74 94	100 200
	CD38 TRDV3	82 49	12 0	64 94	38 109	102 203
	TNFRSF9 FREM2	84 21	10 1	56 111	46 95	102 206
	CD7 PIM2	89 47	7 0	47 96	55 110	102 206
	CASP1 SP140L	68 40	19 0	78 101	26 111	104 212
	GZMB GSTA4	48 7	27 1	95 119	11 96	106 215
	ADAR LINC00944	75 33	14 0	71 105	35 112	106 217
	DTX3L BMS1P20	78 27	12 0	67 107	39 113	106 220
	BTN3A1 COL9A3	100 24	1 0	21 108	85 114	106 222
	IL12RB1 FGD3	84 24	8 0	57 109	50 115	107 224
	FAM26F NKD1	94 21	4 0	38 112	69 116	107 228
	IRF8 C1orf56	43 20	26 0	98 114	12 117	110 231
	RASGRP1 NUTM2A-AS1	95 18	3 0	36 116	75 118	111 234

Table SII .

 SII Comparisons of the clinical variables between the Chinese and French cohort. Unpaired t-test, b χ 2 test, c Wilcoxon's rank sum test and d Log-rank test. 


	Clinical variable	Chinese cohort (GSE71187, n=52)	French cohort (GSE39582, n=585)
	Sex, ratio (n)		P>0.05 a
	Male	0.577 (30)	0.550 (322)
	Female	0.423 (22)	0.450 (263)
	Age, year		P<0.001 b
	Median (range)	58 (26-93)	69 (22-97)
	Tumor-Node-		P>0.05 c
	Metastasis stage, ratio (n)		
	I	0	0.067(39)
	II	0.365 (19)	0.466 (271)
	III	0.635 (33)	0.361 (210)
	IV	0	0.103 (60)
	NA, n	0	2
	Overall survival time, year		P>0.05 d
	Median	3.865	4.333
	Survival state, ratio (n)		
	Died	0.423 (22)	0.331 (194)
	Alive	0.577 (30)	0.658 (385)
	NA, n	0	6

a