Glutaredoxin GRXS13 plays a key role in protection to photooxidative stress in Arabidopsis


Figure S1. Phylogenetic relationship and classification of the 31 GRXs from Arabidopsis. 
The hierarchical cluster analysis of the 31 GRXs predicted in Arabidopsis was performed with the full-length deduced protein sequences obtained from TAIR (www.arabidopsis.org) using the CLUSTAL W software for sequence alignment (http://www.ebi.ac.uk/Tools/clustalw2/index.html). Cladogram was built according to the maximum parsimony algorithm with a bootstrap of 500 iterations. The Arabidopsis thioredoxin gene At1g02920.1 was used as an external group. Clusters of CC-type, CPYC-type and CGFS-type GRX are separated by square brackets. The Arabidopsis Genome Initiative code, the assigned names (between brackets) and the four active-site aminoacids, are indicated for each gene. GRX genes are named according to GRXS/GRXC classiffication and to ROXY classification for the CC-type (Li et al., 2009). GRXS13.1 and GRXS13.2 variant genes are underlined and glutaredoxins previously characterized by genetic approaches are indicated by an asterisk (*). 

Target Gene


Forward Primer

Reverse Primer At1g03850.1 (GRXS13.1) 
CCTCCGCTCAAATCTTACGG CTTTTTCGTAACCTAGTCGG At1g03850.2 (GRXS13.2) CAGTGGTGGTTGAGATTGGTGAAG AGTGCCTACTCTTAGACAAGCTGG At4g24550 (Clathrin) AGCACCGGGTTCTAACTCAA AATACGCGCTGAGTTCCCTT At5g08290 (YLS8) TTACTGTTTCGGTTGTTCTCCATTT CACTGAATCATGTTCGAAGCAAGT At4g05320 (UBQ10) GGCCTTGTATAATCCCTGATGAATAAG AAAGAGATAACAGGAACGGAAACATAG At1g02840 (GRXC9) CCTACATAAACCGCCGGT GAGGCTGCTTCTTGGACTT At1g02930 (GST-1) TAATAAAAGTGGCGACC ACATTCAAATCAAACACTCG
Table S1. Primers used to quantify transcripts from the indicated genes by qRT-PCR. 

Gene variant

ESTs FL-cDNAs At1g03850.1 
EBENXNS02JCUQX RAFL14-01-M01 (3`) RAFL14-01-M01 (5`) U22510 At1g03850.2 06c01 (3`) 07E02 (3`) 1025557 (5`) 271253 (3`) 271253 (5`) 27F7 (3`) 39H8 (3`) EB3RODY01AW7JM EB3RODY01DG3Y4 EB3RODY01DVA3R EB3RODY01E2936 EB3RODY02HOI1F EBENXNS02H8O7Y EBENXNS02JP95O EBENXNS02JS5FE MPIZP200III83Q (5´) RAFL04-13-K21 (3`) RAFL04-13-K21 (5`) RAFL15-03-F10 (3`) RAFL15-03-F10 (5`) RAFL16-63-C05 (3`) RAFL19-43-J01 (3`) R09778 U09778 AY086732 GSLTPGH52ZE12
Table S2. Evidence of expression for GRXS13 gene variants in Arabidopsis databases. 
ESTs and FL-cDNAs associated to GRXS13 gene (At1g03850) were obtained from TAIR database (http://www.arabidopsis.org/cgi-bin/gbrowse/arabidopsis/) and analyzed for variant correspondence. Twenty two out of the 25 ESTs (88%) and 4 out of the 5 FL-cDNAs (80%) corresponded to the GRXS13.2 (At1g03850.2) variant.
Daniel Laporte, Ema Olate, Paula Salinas, Marcela Salazar, Xavier Jordana, Loreto Holuigue
Glutaredoxin GRXS13 plays a key role in protection to photooxidative stress in Arabidopsis