Complete nucleotide sequence of a putative G protein coupled receptor: RDC8


The dog cDNA clone RDC8 was isolated by screening a Xgtl 1 clearly indicates that RDC8 belongs to the seven transmembrane thyroid cDNA library with a partial human cDNA used as probe. 
segments receptors family. This probe was obtained by PCR amplification of human thyroid cDNA using degenerated primers corresponding to consensus of the third and sixth transmembrane regions of the G protein REFERENCE coupled receptor family (1). The human and dog sequences were 

1. Lil,et,F., Parmentier,M., Lefort,A., Dinsart,C., Van Sande,J., Maenhaut,C., more than 90% similar in the amplified region. Sequence analysis Simons,M.J., Dumont,J.E. and Vassart,G. (1989) Science 244, 569-572. 

0CGAOOCOOA

  GcCOCG00 AGAAGOG AGCCGOGCGG CGAGCCGCAGs GTaJCCTG GACTATGAG CGAGCTAG CCMAuTC?T CGAAGCC 100 CTGAAGAGO COCAOTTlCT GGAACTCAG ACTGWCAGT GAAAACQO TT1 ¶OOGAC OCOYCACAG OCAOGCC TnC'OTCC TOTAAGAAG 200 GOOC AGOGOCCOCAG OW OCGO ACYOCAUCTA TOGACCOG GCTYGGCOGG CCCGTGTCCT TCGACCCT GCCACGTCC WAGCATG1CC 300 ACCATGOOCT CCTGGGTTA CACACOGG GAGGOCAC TCOCTGTCT GCCATCCTG GCAATC TTGTGC GCGGGG CTGAACAGCA 400 ACCTGCAGAA OGACCAAC TACT YTVCACTGC GCAGCAC ATTOOCTGG GAAGICCTCOC GATCCCCTTC GOCATCACCA TCAGCACAGG 500 TTCTGWTGT OCCYOCCACA ACTEICCTC CTTGCCTG TTYTaCCTGG TCCTCACTCA GAUCCCAYC TYCAGCC TOCATCOC CATACCOC 600 TACATCOCCA TCATCOC ACTCIVOTAC AATG IGACTOOCAC AAGGCCAAG GOCACATTG COGTCTO GTCGTC TTTOCATTU 700 OCTYGAC:TC CATOCTOG TOGAACAACT GCAGTCAGC C AGAAACTACT CACAGOO OGGAGO CAGOT=OOCC GTCTTCTGA 800 GGATGTGG ccATGAACT ACATOOTA CTACAACTTC TTTMCTT O TCCTGTC GCTGCTGT ATOCTONGT TCTACTTGCG GATCTTCCA 900 ocrooc GACAUCtOM OCAGATOA AGOCAGOCTC QWTOGA GCOGGCTVGGC TOCACOCTOC AGAAGGAGT CA C AAGTCACTOG 1000 OCATCATIGT G C GOCTCCT GGCTAOCCC GCATATCATC AACTOCTITA CCTTCTFTTG CCCAGAGT AGOCAGCC CACTCTOOCT 1100 CATOTACCTO ACCATCG TCTVCCACAC CAATTCT GTIOMCOCT ICATCTATOC CTACCGCATC COTG GCAGACCTT OAG CCTCTCM OCC C ACTIGACT GAGAGAGO AGCC OC AC CAT GTOGCCTAGC 1700 AGGAAGOT TOGAGTCM GAAAGTOGAC GTICACAYC AGAAGCA GCATCAGM AGCACAG CACAGAT CTTOGG C 18W TCOCACYC TOGASCATC TAGAAGTACC ATOMATOT OCAGCAGCTAACTOW CCTGAC TOGOGGO C TCC C COOCTACCAC 1900 ACACAGCAA CACCCTCO AGACTITTCA AGATTOG AGCOTATG COCAGAGO A ITCTTYCT TOTTTICT CrrTrrrr 2000 TCCAGGAGA AATGTTAAAC GTGAAGMTO CCTITITTY TTIW?MAT TA1TITCTMY CCGTOCW CAGOTOTT GATCG COCTAACCT 2100 ACATCC A GCAGAOCAGGAUTCAG ACAOCYT TlACOCCA OCACTYCTT ACACCAYCAG TCCCAGGCC
F Libert', M Parmentier1, A Lefort1, J E Dumont', G Vassart1'
Complete nucleotide sequence of a putative G protein coupled receptor: RDC8