Supplemental Information: Fatty acid oxidation promotes cardiomyocyte proliferation rate but does not change cardiomyocyte number in infant mice



Supplemental Figure 1 .

 1 Infant mouse hearts show evidence for cardiomyocyte cycling, hypertrophic growth and maturation. (A) Total number of cardiomyocytes (CM) in both cardiac ventricles of mice. (B) Confocal images of EdU+ cariomyocytes on tissue sections using Click-iT EdU Alexa Fluor (green) and co-immunostaining with antibody against caveolin1. Arrows point to EdU+Caveolin1+ cells. Graph on the right showing quantification of EdU+Caveolin1+ cells as percentage of total caveolin1+ cells analyzed per field. (C and D) Percentage of binuclear (C) and mononuclear (D) cardiomyocytes (CM) in the heart ventricles of infant mice. (E and H) Expression of indicated genes by qRT-PCR analysis of the mRNA of isolated heart ventricles at indicated time points (n= 5-6 per time point). (F and G) The frequency distribution (F) and mean square areas (G) of the surface area of cardiomyocytes isolated from infant mouse heart ventricles. P value was calculated using one-way ANOVA. 




  Supplemental Table1. qRT-PCR primer sequences used in this study. 


		Forward	Reverse
	Myh6	CCACTTCTCCTTGGTCCACTATG	ACAAACCCACCACCGTCTCA
	Myh7	AAGGGCCTGAATGAGGAGTAGCTC GCAAAGGCTCCAGGTCTGA
	Mef2c	GCCAGCACTG ACATGGATAAG	CCATTGAGGCCCTTCTTTCT
	Gata4	CCGGGCTGTCATCTCACTATG	TTCAGAGCAGACAGCACTGGAT
	Nkx2.5	TGACCCAGCCAAAGACCCT	CCATCCGTCTCGGCTTTGT
	Acta1	TGAAGATGGGTTAAGCGGAG	TTCGTCGCACATGGTGTCTA
	Nppa	GGGTAGGATTGACAGGATTGG	CTCCTTGGCTGTTATCTTCGG
	Nppb	CTGAAGGTGCTGTCCCAGAT	CCTTGGTCCTTCAAGAGCTG
	Acaca	CTGGGACAAAGAACCATCCA	ATAATCTGGATGCCCCCAAG
	Acacb	CCGAGTTTGTCACTCGGTTT	GCATACACTTGACCGCAGC
	Acadm	AGCTCTAGACGAAGCCACGA	TGAGCCTAGCGAGTTCAACC
	Acadl	AACGTCTGGACTCCGGTTCT	CGGGTACTCCCACATGTACC
	Cpt1b	TCTCCATGGGACTGGTCGAT	ACCATGCTGAGAAGTGCCTC
	PParα	CAGTCCATCGGTGAGGAGAG	CTGGAAGCTGGAGAGAGGGT
	Pdk4	CGTTCCTTCACACCTTCACC	GGTCAAGGAAGGACGGTTTT
	Ccnd1	TTCCTCTCCAAAATGCCAGA	AGGGTGGGTTGGAAATGAAC
	Ccnd2	GAACCTGGCCGCAGTCACCC	CGACGGCGGGTACATGGCAA
	Bcl2	GTGGATGACTGAGTACCTGAAC	GAGACAGCCAGGAGAAATCAA
	Bax	GTGGTTGCCCTCTTCTACTTT	CAGCCCATGATGGTTCTGAT
	18S	TCAAGAACGAAAGTCGGAGG	GGACATCTAAGGGCATCAC
Tongtong Cao, Daniela Liccardo, Ryan Lacanna, Xiaoying Zhang, Rong Lu, Brian N Finck, Tani Leigh, Xiongwen Chen, Konstantinos Drosatos, Ying Tian
Supplemental Information: Fatty acid oxidation promotes cardiomyocyte proliferation rate but does not change cardiomyocyte number in infant mice