Cloning and sequence analysis of the cDNA for murine interleukin-6


We isolated and sequenced a cDNA clone of murine interleukin-6 (mIL-6) from an activated murine macrophage library constructed in Xgtl 1. This clone is comprised of 1087 bases and a consensus polyadenylation signal 410 bases 3' to the stop codon and 13 bases upstream of the poly (A) tail. The sequence exhibits an AUG codon which starts at base 32 and an open reading frame of 633 bases coding for a protein of 211 amino acids and a predicted molecular weight of 23,210 daltons. At the nucleic acid level, murine and human IL-6 coding sequences reveal 65 % homology and 41 % homology at the amino acid level. The rat and murine cDNA sequences are 92% identical and the protein sequences are 93 % homologous ( 1-3). EMBL accession no. X54542 

ccaagaacgatagtcaattcagaaaccg ATGAAGTTCCTCTCTGCMGAGACTTCCATCCAGTTGCCTTCTTGGGA 79 M K F L S A R D F H P V A F L G 16 CTGATGCTGGTGACAACCACGGCCTTCCCTACTTCACMGTCCGGAGAGGAGACTTCACAGAGGATACCACT 151 L M L V T T T A F P T S Q V R R G D F T E D T T 40 CCCAACAGACCTGTCTATACCACTTCACAAGTCGGAGGCTTMTTACACATGTTCTCTGGGAAATCGTGGM 223 P N R P V Y T T S Q V G G L I T H V L W E I V 1 64 ATGAGAMAGAGTTGTGCAATGGCMTTCTGATTGTATGAACAACGATGATGCACTTGCAGAMACAATCTG 295 M R K E L C N G N S D C M N N D D A L A E N N L 88 AAACTTCCAGAGATACAAAGMATGATGGATGCTACCAAACTGGATATMTCAGGAAMTTGCCTATTGAAA 367 K L P E I 0 R N D G C Y 0 T G Y N 0 E I C L L K 112 AlTICCTCTGGTCTTCTGGAGTACCATAGCTACCTGGAGTACATGAAGACAACMTTAMAGATAACAAGAA 439 S S G L L E Y H S Y L E Y M K N N L K D N K K D 136 GACAAAGCCAGAGTCCTTCAGAGAGATACAGAAACTCTMTTCATATCTTCAACCAAGAGGTAAAAGATTTA 511 K A R V L 0 R D T E T L I H I F N 0 E V K D V L 160 CATAAAATAGTCCTTCCTACCCCMATUCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGGAGTGG 583 H K I V L P T P I S N A L L T D K L E S 0 K E W 184 CTMGGACCAAGACCATCCAATCATCTTGAAATCACTTGMGAATIICTAAAAGTCAC1TTGAGATCTACT 655 L R T K T I O F I L K S L E E F L K V T L R S T 206 CGGCAAACCtagtgcgttatgcctaagcatatcagtttgtggacattcCtcactgtggtCagaaaatatatcgtgtgtcaggtatctgacttattgftgttc R Q T *
H E Grenett, N L Fuentes, G M Fuller
Cloning and sequence analysis of the cDNA for murine interleukin-6