Nitric Oxide Is Required for Melatonin-Enhanced Tolerance against Salinity Stress in Rapeseed (Brassica napus L.) Seedlings

Gan Zhao 1 , Yingying Zhao 1 , Xiuli Yu 1 , Felix Kiprotich 1 , Han Han 1 , Rongzhan Guan 2 , Ren Wang 3 and Wenbiao Shen 1, * Supplementary Table 1 The sequences of primers for qPCR used in rapeseed. 

Primer name

Accession number Sequence (5′→3′) 
Actin AF111812 F: CTGACCGTATGAGCAAAG R: CCACCGAACCAGAAGGCAGA GAPDH FJ529282 F: TTGGTGACAACAGGTCAAGCA R: AAACTTGTCGCTCAATGCAATC NHX1 AY189676 F: CAATGCAGGGTTTCAAGTA R: AGTCAAAGGTCCCAATGTC SOS2 AY310413 F: CCAGAGGTACTTAATGGCCAAG R: GTGGCCTTGACTGAACAATGTA APX HE574697 F: GAGAAATACGCTGCTGATGAAGA R: CCTACCCGAGCTCAGAAAGC MnSOD EF634058 F: TTGGTTCCTCTGGTGGGTATAGA R: CAGATAATCCGGCCTCACATTC Cu/ZnSOD AY970822 F: CCAACGTCCACGCTGATAAGA R: ACAGGTCCTATAGCCGTTGCA
Supplementary Table 2 The sequences of primers for qPCR used in Arabidopsis. 

Primer name Accession number Sequence (5′→3′)

Actin 2 At3g18780 F: ACAACCGGTATTGTGCTGGA R: GAAGACGGAGGATGGCATGA 
GAPDH At1g13440 F: TTGGTGACAACAGGTCAAGCA R: AAACTTGTCGCTCAATGCAATC SOS1 At2g01980 F: TGCTTGATGAGGGCAGAATA R: ACTTGCGTGGGACAACTTTA SOS2 At5g35410 F: TAGCGGAGAATGTAGAGAGAAAT R: TGCTGTCGCCTGTCAAATAG SOS3 At5g24270 F: TGATTGAAGTAATGGTGGATAAGGC R: AGTCATGTTCTTGATGAGCGATG APX1 At1g07890 F: CTCTGGGACGATGCCACAAG R: CTCGACCAAAGGACGGAAAA APX2 At3g09640 F: GTTCAGGATTCGAGGGTGC R: GAAGAGCCTTGTCGGTTGGT CAT At1g20630 F: TTCCCTGTATTCTTCGTCCG R: GCCTTCCATGTGCCTGTAGT FSD1 At495100 F: AACCAGGTGGTGGAGGAA R: GTAAGCAATGGGAAAGAGCC

Figure S1 .

 S1 Figure S1. NaCl-induced inhibition of root elongation was rescued by three types of NO-releasing compounds, but aggravated by the scavenger of NO. Three-day-old rapeseed seedlings were pretreated with 10 μΜ SNP, 200 μM 2-phenyl-4,4,5,5-tetramethylimidazoline-1 -oxyl-3-oxide (PTIO; a scavenger of NO), 10 μM diethylamine NONOate (NONOate; a NO donor), 10 μM S-nitrosoglutathione (GSNO; a NO donor), 200 μM old SNP (a negative conrol of SNP), and 1 μM melatonin for 12 h, and then transferred to 200 mM NaCl for another 2 d. Afterwards, root elongation was measured. The sample without pretreatment was the control (Con). Values are mean ± SE of three independent experiments with at least three replicates for each. Bars with different letters are significant different at p < 0.05 according to Duncan's multiple range test.