Numbers indicate the location of the oligonucleotide primer with regard to the initiation ATG codon. The gene ide Ssuis ends at position 3426. 


	primer	sequence	position a
	preProIde Ssuis PstI	CTTCTGCAGTAAAAGACGCATG	-504 to -483
	postSSide Ssuis BamHI	ATTGGATCCTGTAACAACTGTATCGGC	94 to 114
	preEndide Ssuis BamHI	TAAGGGATCCTCAAGACAGATTATGGGAGT 3346 to 3365
	postEndide Ssuis EcoRI	AACGGAATTCTATACTTCAACTTATTCAC	3926 to 3945
	Ide Ssuis delh_for_BamHI	TTTCGGATCCCTTGCAAAAGCCAAAGAAAC 1291 to 1310
	Ide Ssuis delh_rev_SacI	AAGGAGCTCTTCAACTTGGGCTACC	1878 to 1898
	ide Ssuis delCforPstI	CTACTGCAGAACGGACTTACGGCGCTAT	702 to 720
	ide Ssuis delCrevBamHI	TGTGGATCCGGCTTTTGCAAGCGGATTG	1284 to1302
	ide Ssuis delCforBamHI	TTAGGATCCTTAAAGAAAAGACGTAAGTA	3406 to 3425
	ide Ssuis delCrevEcoRI	GCTGAATTCATGCCTGATTT	3989 to 4008

a .