p63 Silencing Induces Epigenetic Modulation to Enhance Human Cardiac Fibroblast to Cardiomyocyte-Like Differentiation


Supplemental Table

 S1Beta-actin was used as loading control. Beta-actin was used as loading control. IB, immunoblot; IP, immunoprecipitation 

Figure S1 .

 S1 Figure S1. p63 shRNA validation. A) Western analysis of P63 protein levels in immortalized 


Figure S2 .

 S2 Figure S2. p63 interacts with HDAC1 and p63-TID is required for their interaction. A) 293T 


Figure S3 .

 S3 Figure S3. p63 recruits HDAC1 at cardiogenic gene promoter regions. A) H3K27Ac relative 


Figure S4 .

 S4 Figure S4. Western blot raw images, Figure 4A. FLAG co-immunoprecipitation assay in 293T 


Figure S5 .

 S5 Figure S5. Western blot raw images, Figure 5A. FLAG co-immunoprecipitation assay in 293T 


Figure S6 .

 S6 Figure S6. Western blot raw images, Supplemental Figure S1. A) p63 shRNA validation. 


Figure S7 .

 S7 Figure S7. Western blot raw images, Supplemental Figure S2. p63 interacts with HDAC1 


. qRT-PCR rat and human primers list Rat Primer sequences:

  
	Human Primer sequences:	
	Gene	Forward Primer	Reverse Primer
	cTnT	AGGCTCACTTCGAGAACAGG	ATTGCGAATACGCTGCTGT
	Ryr	ACATCATGTTTTACCGCCTGAG TTTGTGGTTATTGAACTCTGGCT
	Pln	GTGACGATCACAGAAGCCAAGG	TGACAGCAGGCAGCCAAACG
	Actc1	GATTATTGCTCCCCCTGAGCG	GTGTAAGGTAGCCGCCTCAGAA
	Col1a1	GCGAAGGCAACAGTCGATTC	CCCAAGTTCCGGTGTGACTC
	Postn GGCTGAAGACTGCCTTGAATGAC	CGTGGCAGCACCTTCAAAGA
	Gapdh GGCACAGTCAAGGCTGAGAATG ATGGTGGTGAAGACGCCAGTA
	ΔNp63	GGAAAACAATGCCCAGACTC	GTGGAATACGTCCAGGTGGC
	TAp63	AAGATGGTGCGACAAACAAG	AGAGAGCATCGAAGGTGGAG

Table S2 . ChIP-qPCR Primer list:

 S2 
	Gene	Forward Primer		Reverse Primer
	cTnT	AGACGCCTCCAGGATCTGT	TCTTCAACAGCTGCTTCTTCC
	Myh6	GCTGGTCACCAACAATCCCTA	CGTCAAAGGCACTATCGGTGG
	Gja1	GCCAAAGACTGTGGGTCTCA	GAAGGTCGCTGGTCCACAAT
	Col1a1	CATGTTCAGCTTTGTGGACCT	GCAGCTGACTTCAGGGATGT
	Postn	CTCATAGTCGTATCAGGGGTCG	ACACAGTCGTTTTCTGTCCAC
	Gapdh	TCACCACCATGGAGAAGGC	GCTAAGCAGTTGGTGGTGCA
	ΔNp63	GAAAACAATGCCCAGACTCAA	TGCGCGTGGTCTGTGTTA
	TAp63	TGTATCCGCATGCAGGACT	CTGTGTTATAGGGACTGGTGGAC
	Gene	Forward Primer		Reverse Primer
	Gata4_G1 CCAATCCTTCCTCTCTGAACC TCAAGTGTCCGTTGATCTTCA
	Gata4_G2 TGAAGCTCGGGGCTTAAGTG AGCGAAGGATCACCCGATTC
	Gata4_G3 CAGTTCCAGGGCGTCCAATA TCTTCTGCGGGCATGCTAAT
	Tnnt2_T1 GGCAGTGGAGGGTGTAACTG CTCCCACCACCAACTGTCTG
	Tnnt2_T2 TCAAGAGGGACAGCTGGTTT AACAAGTACCCCACGCCATA
	Tnnt2_T3 CCCAAGTCGCAGTGAGTTCT ACGTGATTGACAGGACCACC
	Tnnt2_T4 CCTGCCTCAGTTTCAGTGCT	GTGGCCACATGACTCAGGAC
	Myh6_M1 CACCGTAGCCTATGGCTCTC CGGCACTCTTAGCAAACCTC
	Myh6_M2 CTGGCTCCCCAGAGACAAAG GCAGCTGTTCAGTTCTGTGC
	Myh6_M3 CAGCTGAGCCTATGACCCAC TGAGCAAGCACTAACCCTCC
MS Jaya Pratap Pinnamaneni, PhD Vivek P Singh, MD Mary B Kim, MD Christopher T Ryan, MS Aarthi Pugazenthi, MS Deepthi Sanagasetti, MD Megumi Mathison, PhD Jianchang Yang, MD. Todd K Rosengart, Michael E De Bakey
p63 Silencing Induces Epigenetic Modulation to Enhance Human Cardiac Fibroblast to Cardiomyocyte-Like Differentiation