Figure S1 .Figure S2 .Figure S3 .

 S1S2S3 Figure S1. GRP78 protein levels did not change in basal unstressed breast cancer cells cells. (A) Western blot analysis of MCF7(2) cells that were treated with BOLD-100 at indicated doses for 72 h did not show any change in GRP78 levels compared to vehicle Densitometry ratios (protein: loading control) of bands for all conditions versus vehicle control are shown. (B) GRP87 protein levels were determined in MDA-MB-231 cells by Western blotting. Cells were treated with indicted doses of BOLD-100 and/or olaparib for 72 h. β-tubulin was used as the loading control in both cases. For whole blots, please see Figures S7 and S8.. 


Figure S5 .

 S5 Figure S5. Whole blot for Figure 6 A. Note: This blot was cut and bottom portion was used for gamma-H2AX. For actin, which consistently produces one band, only the 45 kDa band was saved. 


Figure S8 .

 S8 Figure S8. Whole blot for Supplementary Figure S1 B. Note: This blot was first used to detect GRP78, stripped and then used for -tubulin. . 


Table S1 : Top 6 identified disease and functional pathways enriched in differentially expressed (DE) genes in MCF7(2) cells.

 S1 Significantly changed genes following treatment with 100 μM BOLD-100 were ranked by p-value (IPA Core Analysis, QIAGEN). 


	# DE GENES	TOP DISEASE AND FUNCTIONS
	33	RNA Post-Translational Modification, Cancer, Cell-mediated Immune Response
	32	Gene Expression, Connective Tissue Disorders, Developmental Disorders
	31	Drug Metabolism, Endocrine System Development and Function, Lipid Metabolism
	31	Cancer, Cell Cycle, Cellular Assembly and Organization
	31	Embryonic Development, Cell Cycle, Cellular Development
	29	Lipid Metabolism, Small Molecule Biochemistry, Vitamin and Mineral Metabolism

Table S2 .

 S2 Complete list of Reserve Phase Protein Array (RPPA) results with significantly altered proteins in MCF7 cells treated with 100 μM BOLD-100 compared with control. 


	DJ1	0.010	24% increased
	β-Actin	0.010	13% increased
	Annexin-VII	0.010	16% increased
	p27_pT198	0.011	14% decreased
	MAPK_pT202_Y204	0.013	7% decreased
	p70-S6K1	0.013	15% increased
	ATR_pS428	0.014	12% decreased
	TIGAR	0.014	15% increased
	MUC1 (EMA)	0.014	65% increased
	UGT1A	0.015	36% increased
	RBM15	0.015	19% decreased
	FRA-1	0.016	7% increased
	TUFM	0.017	16% decreased
	Bax	0.017	12% increased
	Smad4	0.019	4% decreased
	HER2_pY1248	0.020	6% increased
	MEK1	0.020	7% increased
	PROTEIN NAME Stat3 Rab11 PKCa PDK1 MSI2 Notch3 RPA32 TSC1 p53 14-3-3-beta AMPK-a2_pS345 p38-MAPK Collagen-VI SOD2 c-Jun_pS73 G6PD Smad3 NAPSIN ATM_pS1981 PI3K-p85 TAZ HER2 HER3 ACC1 ATRX PEA-15 Cyclin-E1 PCNA Mcl-1 Raptor TWIST RSK Elk1_pS383 DM-K9-Histone-H3 LC3A-B Rad51 IR-b (INSRB) HSP70 Rb PDK1_pS241 PKC-b-II_pS660 Rock-1 WIPI2 CD44 Ets-1 Jak2 Stathmin-1 ATM Paxillin Oct-4 IRS1 b-Catenin Cyclin-D3 P-Cadherin ERCC5 Transglutaminase p27-Kip-1	p-value 0.021 <0.001 0.021 <0.001 0.022 <0.001 0.022 <0.001 0.022 <0.001 0.023 <0.001 0.025 <0.001 0.026 <0.001 0.026 <0.001 0.027 0.001 0.030 0.002 0.031 0.002 0.031 0.002 0.031 0.002 0.031 0.002 0.032 0.002 0.032 0.003 0.035 0.003 0.038 0.004 0.038 0.004 0.039 0.004 0.042 0.004 0.042 0.004 0.042 0.005 0.043 0.005 0.043 0.005 0.044 0.005 0.045 0.005 0.048	9% increased % Difference in 100 μM BOLD-100 treated cells 4% increased 19% increased 10% decreased 24% increased 5% decreased 16% increased 7% increased 27% increased 12% decreased 12% increased 21% increased 16% increased 20% decreased 22% increased 8% decreased 39% increased 3% decreased 11% decreased 13% increased 6% increased 9% increased 31% increased 24% decreased 13% increased 18% increased 12% increased 15% increased 8% decreased 6% decreased 5% decreased 4% decreased 11% increased 21% increased 6% decreased 9% increased 10% decreased 4% decreased 18% increased 10% increased 35% increased 4% decreased 18% increased 7% decreased 7% decreased 3% decreased 15% decreased 11% increased 18% decreased 16% decreased 6% decreased 39% increased 7% decreased 5% increased 12% increased 5% increased 2% decreased
	D-a-Tubulin	0.005	8% increased
	Notch1	0.006	21% increased
	TFAM	0.006	30% increased
	INPP4b	0.007	19% increased
	Chk2	0.007	8% decreased
	Chk1_pS296	0.007	4% decreased
	14-3-3-zeta	0.009	17% increased
	PR (Progesterone receptor)	0.009	64% decreased

Supplementary Materials