1 Apoplastic Sugar May be Lost from Grape Berries and Retrieved in Pedicels Supplemental Table S1. Sequences of primers and the GenBank accession numbers of the genes used in gene expression analysis. 

Gene

Primer sequence (5'-3') 



  Dissociation kinetics at the end of each PCR run were used to verify specific annealing of the oligonucleotides. The efficiency of each primer pair was quantified on a serial dilution of PCR products. 


			Reference	GenBank
				Accession
				number
	HT1	F: CGTTGTTCACATCGTCGCTTTATC Lecourieux et al., 2010 AJ001061
		R: GAGCAGTCCTCCGAATAGCATTG		
	HT2	F: GGCATAGGGGTGGTGGTAG	Lecourieux et al., 2010 AY663846
		R: TCGGGCTTTACGGAGAGAG		
	HT3	F: GCTACTCTCTACTCGTCCGCTTTG Lecourieux et al., 2010 AY538259
		R: CCCGTCATTGCTTCCGAACTTG		
	HT4	F: GGGCTGGCGAGTTTCTCTAG	Lecourieux et al., 2010 AY538260
		R: AGTTCTGCTTGGACATCGTTTG		
	HT5	F: CAGGCTGTTCCACTGTTCTTATCG Lecourieux et al., 2010 AY538261
		R: AGTTAGGAGGACCGCAGGAATC		
	HT6	F: TTTATTTGCATGAGGAGGGAGTC Lecourieux et al., 2010 AY861386
		R: GAGCAGCAGCCTGGATATAATC		
			Lecourieux et al.,	AY854146
	HT7	F: TTACCTCTTCCCTCTACCTTGCTG	unpublished a	
		R: TCGCCAAATCCAATGCCAATACC		
			Lecourieux et al.,	AF021808
	SUC11 F: CTCACGCCTGGTCCAGTATC	unpublished	
		R: CCGATGTCAGCCGAGAATCC		
			Lecourieux et al.,	AF021809
	SUC12 F: CAAGAATCTGAAGCAGGGTGAG	unpublished	
		R: GGCGGCAATCATACAACTGAG		
			Lecourieux et al.,	AF021810
	SUC27 F: GGAGTTAGCCAAGCCTTCTTCAG	unpublished	
		R: GCAACGCCCATCCGAACTG		
	Actin	F: CTTGCATCCCTCAGCACCTT	Reid et al., 2006	EC969944
		R: TCCTGTGGACAATGGATGGA		
	GAPDH F: TTCTCGTTGAGGGCTATTCCA	Reid et al., 2006	CB973647
		R: CCACAGACTTCATCGGTGACA		
	a Specific primer pairs were designed with Beacon Designer 7 software (Premier Biosoft
	International).		

a 
Supplemental Figure S1. Pedicel cross-section of a green hard Merlot grape berry. 
Samples were cut at 0.8 µm thickness and stained with 1% Toluidine blue in 1% sodium borohydrate for 2 min with heat, and the picture was taken under a light microscope. 
Abbreviations: COR, cortex; P, phloem; X, xylem. Bar = 100 μm.