<i>In Vitro</i> Effects of Lead on Gene Expression in Neural Stem Cells and Associations between Up-regulated Genes and Cognitive Scores in Children



Figure S1 .

 S1 Figure S1. Histone H3 ChIP followed by PCR amplification of the putative SPP1 ARE. NRF2 was activated by KEAP1 knockdown (H3 si). NT-siRNA was transfected into control cells (H3 NT). Following H3 ChIP, qPCR was done to measure the presence of the SPP1 ARE. 


Table S1 .

 S1 Primers Used in Study 


	Target	Application	Forward	Reverse
	DIO3OS	QPCR	GCTACAGGCTTCAGCAGGTC	CCCCAGACAGCAGAGAAATC
	F2RL2	QPCR	GCAAAGCCAACCTTACCCATT	GAGGTAGATGGCAGGTATCAGT
	FTL	QPCR	CAGCCTGGTCAATTTGTACCT	GCCAATTCGCGGAAGAAGTG
	HMOX1	QPCR	GGCAGAGGGTGATAGAAGAGG	AGCTCCTGCAACTCCTCAAA
	LUCAT1	QPCR	CGATCTCGGTTCACTGCAA	AGCAAACAGCAAGTTGGATTC
	NQO1	QPCR	GAAGAGCACTGATCGTACTGGC	GGATACTGAAAGTTCGCAGGG
	OSGIN1	QPCR	CGAGGGTGTCGAGAAGGTG	TCCACGTCAATGGGGTTCCT
	SPP1	QPCR	GGAGTTGAATGGTGCATACAAGG	CCACGGCTGTCCCAATCAG
	KEAP1	QPCR	CTGGAGGATCATACCAAGCAGG	GGATACCCTCAATGGACACCAC
	NRF2	QPCR	TCCAGTCAGAAACCAGTGGAT	GAATGTCTGCGCCAAAAGCTG
	SPP1-a	QPCR	ATCTCCTAGCCCCACAGAAT	CATCAGACTGGTGAGAATCAT
	SPP1-b	QPCR	ATCTCCTAGCCCCAGAGAC	AAAATCAGTGACCAGTTCATCAG
	SPP1-c	QPCR	TGAGGAAAAGCAGAATGCTG	GTCAATGGAGTCCTGGCTGT
	NQO1 ARE	ChIP-QPCR	CCCTTTTAGCCTTGGCACGAAA	TGCACCCAGGGAAGTGTGTTGTAT
	SPP1 ARE	ChIP-QPCR	ATAGGTAGGCTGGGCGATTT	GTGGTTCTGAATTCCGCTGT
	GAPDH	QPCR	GGAAACTGTGGCGTGATG	AGTGAGCTTCCCGTTCAG

Table S2 .

 S2 Characteristics of the ELEMENT cohort 


	Trait	Cohort
	Number of participants	462
	Sex (male/female)	245/217
	Mean gestational age in weeks (sd)	38.38 (1.65)
	Mean maternal age in years at birth (sd)	27.81 (5.51)
	Percentage of mothers married	80.3%
	Percentage of children with siblings	53.0%
	Percentage of mothers who completed high school education	58.4%
	Mean second trimester maternal blood Pb level in ug/dL (sd)	3.76 (2.62)
	Mean Raw Cognitive Development Index at ~24 months (sd)	92.48 (8.33)
	Mean Raw Language Development Index at ~24 months (sd)	89.76 (9.08)
	Mean Raw Psychomotor Development Index at ~24 months (sd)	93.95 (10.58)

Supplemental Material


In Vitro Effects of Lead on Gene Expression in

was activated by KEAP1 knockdown (H3 si). NT-siRNA was transfected into control cells (H3 NT). Following H3 ChIP, qPCR was done to measure the presence of the SPP1 ARE. 

Additional File


Supplemental Code and Data ZIP File

Excel File Table S1. Full results from the differential expression analysis
Peter J Wagner, Hae-Ryung Park, Zhaoxi Wang, Rory Kirchner, Yongyue Wei, Li Su, Kirstie Stanfield, Tomas R Guilarte, Robert O Wright, David C Christiani, Quan Lu
<i>In Vitro</i> Effects of Lead on Gene Expression in Neural Stem Cells and Associations between Up-regulated Genes and Cognitive Scores in Children