Nucleotide sequence of the gene encoding Rhesus monkey (Macaca mulatta) interleukin-3


EMBL accession no. X51890

A clone RDP1. 1 (1) containing the Rhesus monkey IL-3 (RhIL-3) gene was isolated from a X Charon 40 library by screening with radiolabeled human IL-3 (hIL-3) cDNA (2). A 4.1 kb EcoRI fragment of RDP1.1 included the RhIL-3 promoter region containing regulatory elements, identical to those of the hIL-3 gene (3), as well as 5 exons which encode RhIL-3. The isolated gene encodes functional RhIL-3 (1, 4). Comparative studies in vitro between hIL-3 and RhIL-3 produced by B. licheniformis showed that RhIL-3 was about 100-fold more effective in stimulating Rhesus monkey bone marrow cells (1). Similar data were obtained from in vivo studies (4). The availability of RhIL-3 enables further preclinical studies in Rhesus monkeys with homologous IL-3. 

E X N L R R S N L E A F S K A V K S L Q N A S A I E S I L K GGCCCTGCCCTGCCTCTGGGGAGGATGACAGCAGGGCCCACTCCCTTTCCAAGGGAATCTCTGACCATCTGCTTTGATCTCTTTCCACAGAATCTCCCACCATGCCTGCCCATGGCCACGGCCGCACCCACGGTAAGCTGTCCCCCAAGA N L P P C L P M A T A A P T TGCCCGTGTCGTGGCTTGCTCCTCAGTTGGTCATCACCATTATAGCCTGGACTCACCTAATCCCACCTTCTTGGTTTCTTTATA( GAAAACCCTTGAGAATGAGCAGGCTCAATAGACGACTCTGAGCCTCGAGATCTCTTGAGTCCAACGTCCAGCTCGTTCTCTGGGI K T L E N E Q A Q * 3150 ,GCGACCTCCAATCCGTATCACGAACGGTGACCGGAATGACTTCCGGAGGAAACTGAAGTTCTATCT 3300 R P P I R I T N G D R N D F R R K L K F Y L ,TCTTCTCACCACAGAGCTTCAGGACATTGAAAACTTCTGAAACCTCTGGCTCATCTCTCACACAGT 3450



  L P V L L L L H L L V S P G L Q A P M GACCCAGACAACGTCCTTGAAGACAAGCTGGGCTAAGTGCTCTAACATGATCGATGAAATTATAACACACTTAAACCAGCCACCTTTGCCTTCACCGGTGAGTAGCTGGGATAAGACTGGCCTGCAGCAGGGAGGGGTGGTGGCTGCCTC T 0 T T S L K T S W A K C S N M I D E I I T H L N Q P P L P S
H Burger, L C J Dorssers, R W Van Leen, G Wagemaker
Nucleotide sequence of the gene encoding Rhesus monkey (Macaca mulatta) interleukin-3