Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis 2



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 10 Norway To whom correspondence should be addressed: (Tel: +31 50 363 2111, Fax: +31 50 363 2348, Email: 11 jan.kok@rug.nl) 


Table S1

 S1 Primers used in this study 


	13		
	Oligonucleotide * Sequence; (5'-3')	Restriction
			Enzyme
	FbIL285	CTTCACGAATGGGCCCCAATAGTTATGATTGC	
	FbIL286	CCTTGCGAATGGGTCCTAGAAGTTATGAC	
	FbIL309	CTTCACGAATGGGTCCAAAAAGTTATGATTGTAG	
	RbIl285	TCAGTATTACCCATTGAGCCAGCTGACAG	
	RbIL286	TCAGTATTACCCATTGAACCTGCTGACAG	
	RbIL309	GTTTCAGTATTACCCATTGAACCTTCTGACAG	
	bil286F	ATATGGTCTCCCATGTCAAGTATTGAAAATATGATTGC	BsaI for NcoI
			overhang
	bil285F	ATATGGTCTCCCATGCCAAGTATTGAAAATATGATAGC	BsaI for NcoI
			overhang
	bil286R	ATATGGTCTCCAGCTTTATAATTTATTCGCATTTAATCGACG BsaI for HindIII
			overhang

* F and R indicate forward and reverse primers of specific endolysin genes, respectively. 
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Ram R Ganesh, Visweswaran, Dorota Kurek, Monika Szeliga, Francisco Romero Pastrana, Oscar P Kuipers, Jan Kok, Girbe Buist
Expression of prophage-encoded endolysins contributes to autolysis of Lactococcus lactis 2