Fig. S1 -Wilson & Edgell 


TABLE S1 .

 S1 Oligonucleotides used in this study. 


		mobE / mobE	C			
		mutations				
	Oligo	Purpose segment 2	5'-3' Sequence	Strand	Position	Phage
		reverse			Relative to	
	DE-369	To clone StuI	AATTCCGGCAGGCCTCGG		td start	N/A
		site into EcoRI			codon	
	DE-30	mobE 3' end for digested pCR2.1	CGGGATCCACTCATTTAGAATCCT	-	5397	T4
	DE-370	mutant To clone StuI	TAAATTTAC AATTCCGAGGCCTGCCGG			N/A
		sequencing site into EcoRI				
	DE-193	RNA Adaptor digested pCR2.1	GCUGAUGGCGAUGAAUGAACAC			N/A
	DE-387	5'/3' RACE (5' 3'-RACE nrdA /	UGCGUUUGCUGGCUUUGAUGAA GAAACTCCTAAAGCCGATGATTG	+	4656	T4
		phosphorylated) RT-PCR across	A TG			
	DE-194	3'-RACE mobE terminator	TTTCATCAAAGCCAGCAAACGC			N/A
	DE-388	adaptor specific mobE phage	ATTATTCAGACACTCGTTGGTCTC	+	4114	T4
	DE-195	3'-RACE mutations	CAAACGCAGTGTTCATTCATCGCC G			N/A
	DE-412	adaptor specific I-TevI specific	GAGGCCTGAGTATAAGGTG	+	555	T4
	DE-196	5'-RACE probe (intron)	GCTGATGGCGATGAATGAACACT			N/A
	DE-413	adaptor specific I-TevIII specific	G TGCGCCTTTAAACGGTTAG	+	6060	T4
	DE-197	5'-RACE probe (intron)	ACACTGCGTTTGCTGGCTTTGATG			N/A
	DE-416	adaptor specific Sequencing	TCGGTGTAGGTGTTACCAACTATG	+	3958	T4
	DE-251	mobE specific progeny phage	CTTCTGTTAAACGCAGACCTG C	-	5078	T4
		probe nrdA and T4				
	DE-256	mobEΔ33-42 mobE phage	GAAAAATGCGAATATAACAGATT	-	4912	T4
		segment 1 mutations	TTACCGGGCATCTAGGGATTATAT			
	DE-421	mobE 5' end for	GATG TGAGCTCGGGAAATTAGGTC	-	4740	T4
	DE-257	mobEΔ33-42 progeny phage	CCGGTAAAATCTGTTATATTC	+	4883	T4
		segment 2 sequencing				
	DE-259 DE-422	T4 mobEΔ33-42 mobE 3' end for	TCCCTAGATGCCCGGTAAAATC CAGCAGAGTATCCACCTTGC	+ +	4785 5296	T4 T4
		detection progeny phage				
	DE-352	RNase sequencing	GTAATACGACTCACTATAGGCCG	-	4856	T4
	DE-423	protection mobE I-TevIII 5' end	TACTTCTGAACCTATAAAATGCTC TTCCATAAAGGACTTTCCAGC	-	6122	T4
		terminator + T7 for progeny	TCTAG			
		promoter phage				
	DE-353	RNase sequencing	CGTACTTCTGAACGTTCTGGTACT	+	4630	T4
	DE-424	protection mobE I-TevIII 3' end	GATGATTAT GCAAAACAAGGTTCAACGAC	+	6496	T4
		terminator for progeny				
	DE-363	nrdA northern phage	CTTATCTCCGTGATGGAATGACA	+	2566	T4
		probe sequencing	AC			
	DE-364 DE-465	nrdA northern T2 specific	TTGATGCAGTGCCATTCCAATAGT AAGCTGAAGATTGTTCATCC	-+	2972 3655	T4 T2
		probe primer extension	C			
	DE-365 DE-488	RT-PCR mobE / mobE northern for nrdB 5' end Progeny nrdB	AACAGCTAGAGAGCATTTTATAG CG CATCAACATCATTATCAATCTGTG	+ -	4834 7121 / 4881 T4/T2 T4
		probe sequencing	C			
	DE-366 DE-522	nrdA 3'-RACE Primer	TTCCAAAAGGAAAGGTTCCAATG CAGAGCCCTGAGGAAGCTTGTTA	+ -	4531	T4
		Extension +SacI	TC AATTGC			
	DE-367	RT-PCR mobE site	TCTTGTCGGAGAAATTGTACCATA	-	5239	T4
		terminator /	C			
		mobE northern				
		probe				
	DE-368	5'-RACE Outer ATACTCTGCTCCTTGTTTAGTTCT -	5305	T4

TABLE S2 .

 S2 Phage-to-phage crosses. 


			%T4				
	Phages	Exp	Input	N	% mobE % I-TevIII % I-TevI
	T2 x T4	1	47.2	211	86.7	84.8	91.4
		2	48.1	216	97.7	97.7	96.3
		3	38.9	216	89.4	91.2	95.3
	T2 x T4ΔmobE33-62 1	51.9	216	64.8	64.8	100
		2	44.4	216	81.9	82.4	99.5
		3	49.1	216	71.7	N.D.	N.D.

Supplemental data for

Lanes 1-3, primer extension on DraI-digested gDNA, with increasing amounts of extension reaction added from left to right. Lanes 4-7, primer extension on DNA isolated after co-infection with T2 and T4. Note that the predicted size of the primer extension product is ~78 nts.