Table S1 -List of oligonucleotide staples required to construct SPOTs and their precursors or derivatives, related to STAR Methods. This table is also available as a CSV file for ordering from commercial oligonucleotide suppliers (Table S4). All oligonucleotides require a standard desalting purification unless indicated. 42[107]41[107 
TTTTTTAACATCCAATAAATCATAGCTAAACGGGGAGCGTATTG 1 nmol A-4[111]1[115] AAAGTACCGAAGGCACCAACCGACTAAAGTTA 1 nmol A-4[155]3[161] CGCCACTCAAAAACACCCTCAGAA 1 nmol A-4[181]2[168] ATCACCGGCCTATTCAGCTTGCTTTCGAAGTAAATAAGTTTT 1 nmol A-4[222]2[210] CCGTCGGAGGCTGAAAAAAAGGCTCCAAACAGTTTAGCATT 1 nmol A-40[107]39[107] TTTTTTCCAAGATTAAGCAAAAACGGGTATTTTT 1 nmol A-40[76]71[92] AGCATAAACAGGCACATCAATTTTGGGGGAATGGCTAATGCGCGAACTGATAGCTTTT 1 nmol A-41[47]41[76] TTTTAAAACATTATGACCCTGTAATACTTT 1 nmol A-

Table S2 -

 S2 Mixtures of staples required to produce SPOTs and their derivatives or precursors, related to STAR Methods. 


		Required Staple Mixes	
	Construct	from Table S1	Required Additional Reagents
	Signpost origami (SPO)	1, 2, 4, 6	-
	SPO with Texas red label	1, 2, 5, 6	Texas red labeled oligo
	SPO with cy5 label	1, 2, 5, 6	cy5 labeled oligo
	SPOT (SPO with aptamer)	1, 2, 4, 7, 8	Transcribed apatmer
	SPOT with Texas red label	1, 2, 5, 7, 8	aptamer, Texas red label
	SPOT with cy5 label	1, 2, 5, 7, 8	aptamer, cy5 label
	SPOT with rigid aptamer attachment	1, 3, 4, 8	rigid aptamer

Table S3 -

 S3 Primers used for cloning of fluorescent protein genes, related to STAR Methods. 


	Description	Primer	Sequence
		type	
	Primers for insertion of fluorescent	forward	GGCGGGAATTCATGGTG
	protein genes in pEP98-gB		AGCAAGGGCGAG
		reverse	GGCGGCTCGAGCTTGTA
			CAGCTCGTCCATGC
	Primers for insertion of TEV	forward	GGCGGGAATTCCGCCGA
	restriction site as linker into mCherry-		CGCCACCAGG
	gB in pEP98	reverse	gBGGCGGCTCGAGAACC
			TGTACTTCCAGTCCGCTC
			CGACTTCCCCCGG
	Primers for ligation independent	forward	CACCACCACCACTGA
	cloning of fluorescent proteins into	reverse	AGTGGTGGTGGTGCTTAC
	pET-28, plasmid		TTGTACAGCTC
	Primers for ligation independent	forward	AGATTGGTGAGAATTTAT
	cloning of fluorescent proteins into		ATTTTCAGGGCCATATGG
	pET-28, insert		TGAGCAAGG
		reverse	AGTGGTGGTGGTGCTTAC
			TTGTACAGCTC