FOXO1-suppressed miR-424 regulates the proliferation and osteogenic differentiation of MSCs by targeting FGF2 under oxidative stress


CAGCAGCAATTCATGTTTTGAAGTGTTCTAAATGGTTCAAAACGTGAGGCGCTGCTATACCCCCTCGTGGGG AAGGTAGAAGGTGGGGTCT) miR424 sequence

We obtained the human miR-424 promoter and predicted FOXO1 binding sites using the JASPAR database (http://jaspar.genereg.net/cgi-bin/jaspar_db.pl?rm=browse&db=core&tax_group=vertebrates ) . Accordingly, three primers were designed and then PCRs were performed for the analysis of FOXO1 binding to the promoter of miR-424. The oligonucleotides were as follows: 
The primer at -1559bp that encompass FOXO1 BS1 of the human miR-424 promoter (5'to 3'): 



  The primer at -1418bp that encompass FOXO1 BS2 of the human miR-424 promoter (5'to 3'):Forward: TGTTGGGAGAAAGTTGTGGA Reverse: CCATCTTGTTTTGGCAATGA The primer at -412～-443bp that encompass FOXO1 BS3 of the human miR- 




  


Supplementary Figure 1: FOXO1 mRNA levels after the knockdown of FOXO1 alone and the concomitant knockdown of FOXO1 and miR-424
Liangping Li, Qihua Qi, Jiaquan Luo, Sheng Huang, Zemin Ling, Manman Gao, Zhiyu Zhou, Maik Stiehler, Xuenong Zou
FOXO1-suppressed miR-424 regulates the proliferation and osteogenic differentiation of MSCs by targeting FGF2 under oxidative stress