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# (12) United States Patent Yajima et al. 

# (54) PROCESS FOR PRODUCING COENZYME QlO 

(71) Applicant: KANEKA CORPORATION, Osaka-shi (JP) 

(72) Inventors: Kazuyoshi Yajima, Hyogo (JP); Takahisa Kato, Hyogo (JP); Akihisa Kanda, Osaka (JP); Shiro Kitamura, Hyogo (JP); Yasuyoshi Ueda, Hyogo (JP) 

(73) Assignee: KANEKA CORPORATION, Osaka (JP) 

( *) Notice: Subject to any disclaimer, the term ofthis patent is extended or adjusted under 35 U.S.C. 154(b) by O days. This patent is subject to a terminal dis- claimer. 

(21) Appl. No.: 15/076,025 

(22) Filed: Mar. 21, 2016 

## (65) Prior Publication Data 

US 2016/0304915 Al Oct. 20, 2016 

(62) Division of application No. 13/020,500, filed on Feb. 3, 2011, now Pat. No. 9,315,839, which is a division of application No. 11/981,181, filed on Oct. 31, 2007, now Pat. No. 7,910,340, which is a division of application No. 10/500,249, filed as application No. PCT/JP02/13766 on Dec. 27, 2002, now abandoned. 

## (30) Foreign Application Priority Data 

Dec. 27, 2001 (JP) ................................. 2001-398545 

(51) Int. Cl. 

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(52) U.S. Cl. CPC .. C12P 7166 (2013.01); C12P 7122 (2013.01) 

( 58) Field of Classification Search CPC ......................................................... C12P 7/66 

See application file for complete search history. 

## (56) References Cited 

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## Related U.S. Application Data 

## U.S. PATENT DOCUMENTS 

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# (IO) Patent No.: US 9,926,580 B2 

# (45) Date of Patent: *Mar.27,2018 

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Wakabayashi et al. Biol Pharm Bull, 1994, 17(8): 998-1002.* 

Yoshida et al. J Gen Appl Microbiol., 1998, 44, 19-28.* 

Takada, Masahiro et al "Studies on Reduced and Oxidized Coen› zyme Q (Ubiquinones): The Determination Oxidation-Reduction LEvels of Coenzyme Q in Mitochondria, Microsomes and Plasma by High-Performance Liquid Chromatography," Biochimica et Biophysica Acta, 1982, vol. 679, pp. 308-314. 

Yoshida, Hajime et al "Production ofubiquinone-10 using bacteria," J. Gen. Microbiol. 1998, vol. 44, pp. 19-26. 

Disch, Andrea et al., "On the Absence of the Glyceraldehyde 3-Phosphate/Pyruvate Pathway for Isoprenoid Biosynthesis in Fungi and Yeasts," FEMS Microbiology Letters, vol. 168, No. 2, 1998, pp. 201-208. 

Kockova-Kratochvilova, A. et al., "Die Beziehungen innerhalb der Gattung Cryptococcus (Sanfelice) Vuillemin", Zbl. Bakt. Abt. 11, Bd., vol. 131, No. 7, 1976, pp. 610-631. 

Natori, Y. et al., "Production of Coenzyme Qlo by Pseudomonas N842", Agric. Biol. Chem., vol, 42, No. 9, 1978, pp. 1799-1800. 

Primary Examiner - Vera Afremova 

(74) Attorney, Agent, or Firm - Westerman, Hattori, Daniels & Adrian, LLP 

## (57) ABSTRACT 

A process for producing on an industrial scale the oxidized coenzyme Q$_{10}$ , includes culturing a reduced coenzyme Q 10 - producing microorganism selected from the group consist› ing of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q$_{10 }$at a ratio of not less than 70 mole% among the entire coenzymes Q$_{10}$; and one of: (a) oxidizing thus-obtained reduced coenzyme Q$_{10 }$ to oxidized coenzyme Q$_{10 }$ and then extracting the oxidized coenzyme Q$_{10 }$by an organic solvent; or (b) extract› ing reduced coenzyme Q$_{10 }$by an organic solvent and oxi› dizing the extracted reduced coenzyme Q$_{10 }$ to oxidized coenzyme Q$_{10 }$. 

US009926580B2 

## FOREIGN PATENT DOCUMENTS 

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## OTHER PUBLICATIONS 

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## 17 Claims, 1 Drawing Sheet 

