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slowly added, producing a clear solution. After sitting at 50° C. for 24 hours, the solution was analyzed to determine the presence of a peracid. 

To determine the presence of a peracid, a kinetic iodomet- ric titration similar to the method disclosed in Sully and 5 Williams ("The Analysis of Per-Acids and Hydrogen Perox› ide," The Analyst, 87: 1037, p. 653 (August 1962)) was used. This method has demonstrated a lower detection limit of about 0.3 ppm for POAA. Given the molecular weight ratio of POAA to the perspective percarboxylic acid of PC-48, the detection limit was estimated to be about 1.4 ppm (3.93xl0- 6 M). No peracidformation was observed. This is equivalent to 10 a percarboxylic acid formation constant (Keq) less than 0.002, suggesting substantially no peracid was formed. 15 

Alternatively, formation of the peracid was determined using $^{13}$C NMR (D$_{2}$O). Using this technique, no carbonyl resonance signal from the peracid was observed. 

Other a-sulfonated fatty acid sources such as Alpha-Step PC-48 and Alpha-Step BSS-45 were also reacted with H$_{2}$O$_{2 }$ in a similar manner, and in both cases, no corresponding peracids were detected. 

Non-a-sulfonated fatty acids were also tested to determine the likelihood of peracid formation. For the sulfonated oleic acid discussed above, the measured formation constant was 1.42. The sulfonated undecenoic acid was collected as a stable solid powder, so the formation constant was not mea› sured. Although the formation constant of the peracid of sulfonated oleic acid is significantly lower than that of the most common commercialized peracid, peroxyacetic acid (Keq=2.70), it is still high enough to make practical yields. 

Overall, without wishing to be bound by any particular theory, it is thought that the a-sulfa group prohibits the oxi› dation and/or perhydrolysis of the carboxylic acid group by H$_{2}$O$_{2 }$to the corresponding peracid. This may in part be due to its strong electron withdrawing effects. 

A study was run to determine the efficacy of compositions of the present invention as sanitizers used in a clean in place cleaning method. A composition including about 5.85 wt% of the sulfonated peroleic acid product, and about 11.6% hydrogen peroxide, about 1 wt % of a chelating agent, about 12.75 wt% ofH$_{2}$SO$_{4}$ , about 13.6 wt% NAS-FAL (sodium octane sulfonate ), and about 1.5 wt% of SXS ( commercially available from the Stepan Company) was prepared. Synthetic hard water was used to dilute the test composition to the desired peracid concentration. The peracid was tested at con› centrations of 1000 ppm, 750 ppm and 500 ppm. The pH of the use solutions were as follows: 

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The use solutions were tested against Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442. The organic soil used was 5% Fetal Bovine Serum. The exposure time of the test was 5 minutes at a temperature of20–1 ° C. A neutralizer screen was also performed as part of the testing to verify that the neutralizer adequately neutral› ized the product and was not detrimental to the tested organ- 

## Clean in Place Sanitizing Compositions 

## Example 9 

isms. The plates were incubated at 35° C. for 48 hours with the test systems prior to exposure to the peracids. The results are shown in the table below. 

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As can be seen from these results, the use solutions tested were effective disinfectants against both Staphylococcus aureus, and Pseudomonas aeruginosa at the concentrations tested. 

Another study was run to determine the sanitizing efficacy of the test solution against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 11229 after a 30 second exposure time. For this experiment the solutions were diluted 

