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about thirty hours. Next, pour half of the provided salt into one container and stir the solution until the salt dissolves. Pour some of this solution into the prawn hatchery. Place a few eggs close to the lid. Position the hatch- ery somewhere with plenty of light but not in direct sunlight. The ambient temperature should ideally hover around 77°F. As water in the hatchery evaporates, gradually add fresh water from the second container. After two to three days, the eggs will hatch prawn larvae, called nauplii. 

## Observe Your Brine Shrimp 

The animal that hatches from the egg is known as a nauplius larva. With the help of a pipette, you can place a few of these larvae on a glass slide and observe them. The larvae will move around in the salt water by using their hair-like appendages. Take a few larvae from the container each day and observe them under the microscope. Remember to return them to their container when you’re done observing them. In case you’ve hatched the larvae in a hatchery, sim- ply take off the cover of the tank and place the tank on the stage. Depending on the room temperature, the larvae will be mature in 6-10 weeks. Soon, you will have raised a whole generation of brine shrimp, which will constantly grow in numbers. 

## Feed Your Brine Shrimp 

Feed your brine shrimp often to keep them alive. The best food is dry-powdered yeast. Give the some every other day. Be careful not to overfeed them, as doing so can cause the water to stagnate and poison the shrimp. If the water does begin to stagnate (you’ll see it darkening), transfer the shrimp to a freshwater solution. 

## Warning! The shrimp eggs and the shrimp are not meant to be eaten! Experiment 7: 

Developing Bread Mold Object: An old piece of bread Put the bread on a slide and lightly moisten it with water. Place the bread into a sealed container, and keep it warm and out of harsh light. Within a short time, black bread mold will form. When the mold takes on a white, shiny appearance, remove the slide from the con- tainer and observe it with your microscope. It will look like a complicated mass of thread, forming the fungus body, which is called the mycelium. Each thread is known as a hypha. These threads, or hyphae, grow like long, slim stacks, ending in a small, white ball, called a 

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sporcap. Inside the sporcap is a spore that will eventually be released to start new colo- nies of mold. With your microscope you can watch this amazing transformation unfold. 

## Experiment 8: 

Observing Stem and Root Sections Objects: 

1. A celery stalk 

2. A carrot Cut several very thin slices from the middle of the celery (a stem) and from the middle of the carrot (a root). Make a wet mount by placing a drop of water on the slide. Then put the specimen on the water-covered sli- de, and top it with a cover slip. The water will help support the sample. It also fills in the space between the cover slip and the slide. Start by viewing the specimens at the lowest magnification and then increase the magni- fication, for more detailed observation. What differences are there between the stem and the root? 

## Experiment 9: 

Observing Cork Cells Object: A small cork With an adult’s supervision, cut a very thin slice from the cork. The thinner you cut the slice, the better you‘ll be able to observe it.. Prepare a wet mount of this cork slice as you did with the celery and carrot in Experiment 8. When applying the cover slip over the sli- de, the water, and the cork, make sure no air bubbles are trapped beneath it. Begin ob- serving the specimen with the lowest power, and increase the magnification as desired. The cells you see, called lenticels, are actu- ally the air pockets that are left after the plant material inside has died. 

## Experiment 10: 

Observing Leaf Cells Objects: A fresh leaf, clean and dry, without holes or blemishes With an adult’s supervision, cut a one-inch -wide cross section out of the center of the leaf, from one side of the leaf to the other. Tightly roll that section up, starting from one uncut edge of the leaf. The central vein of the leaf will be in the center of the roll and not be visible. Then cut several very thin slices off of one end of the roll. The central vein will be in the middle of this almost transparent slice. You’ll be observing the cells around that central vein. Using a droplet of water, make a wet mount (as in Experiments 8 and 9), placing the leaf segment so that the inner part faces up. Start with the lowest power 

## 1200X Microscope Manual 

and gradually increase the magnification for more detail. What do you observe about the leaf cells? 

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