content_image/937716.jpg
# c19) United States 

# c12) Patent Application Publication Keough et al. 

# (54) NEW METHODS AND KITS FOR SEQUENCING POLYPEPTIDES 

(75) Inventors: Thomas Woods Keough, Cincinnati, OH (US); Robert Scott Youngquist, Mason, OH (US) 

Correspondence Address: Bart S. Hersko The Procter & Gamble Co. Miami Valley Labs P. 0. Box 538707 Cincinnati, OH 45253-8707 (US) 

(73) Assignee: The Procter & Gamble Co. 

(21) Appl. No.: 09/906,481 

(22) Filed: Jul. 16, 2001 

(60) Provisional application No. 60/220,564, filed on Jul. 25, 2000. 

## Related U.S. Application Data 

<img src='content_image/937716.jpg'>

US 20060141632Al 

# c10) Pub. No.: US 2006/0141632 Al 

# (43) Pub. Date: Jun. 29, 2006 

(51) Int. Cl. GOIN 33/00 (2006.01) 

(52) U.S. Cl. ................................................................ 436/89 

## (57) ABSTRACT 

The present disclosure provides methods and kits which are useful for sequencing polypeptides. The methods involve derivatization of the N-terminus of the polypeptide or pep› tides thereof. The methods also involve derivatization of the epsilon amino group of the side-chain of the lysine contain› ing polypeptide or peptides thereof. Mass spectral analysis of one or more of the resulting derivatized analytes provides spectra which are readily interpreted through the use of techniques well-known to the ordinarily skilled artisan. The present disclosure also describes kits which enhance con› venient performance of the methods. 

## Publication Classification 

