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Discussion 

[0415] The aflibercept formulations of Block Dare physi› cally and chemically stable. 

[0416] It is further contemplated that in the formulations of Block D, phosphate could be used instead of histidine buffer. It is also contemplated that CaC1$_{2 }$ could be used instead of, or in addition to, MgC1$_{2}$ . 

## Example 6-Buffer-Free Aflibercept Formulations with Alternative Stabilizers 

[0417] This example describes experiments to evaluate buffer-free aflibercept formulations that have a stabilizer other than sucrose (Block E). The design mirrored Block D, except all of these compositions include 0.03% PS 20. This will allow for a direct and detailed examination of the importance PS 20 (and co-solvents in general) for their impact on storage stability. Table 58, below, shows an exemplary set of such formulations. 

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Concentration, pH, and Osmolality 

[0418] The aflibercept concentration (mg/mL), pH, and osmolality (mOSm) of the Block E formulations were evaluated at tO. Results are reported in Table 59 below. 

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Size Exclusion Chromatography 

[0419] The stability of the Block E formulations was evaluated by measuring the amount of non-degraded afliber› cept present in the formulation at time O (tO) and after being subjected to storage conditions. Size Exclusion Chromatog› raphy was conducted on the formulations and the percentage of protein present in the main peak (non-degraded afliber› cept) in each formulation is reported in Table 60-63 below. 

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