(saccharose/sucrose) is driven by brush border enzymes. The effect of the test product containing phenols on the brush border enzymes and hence digestion of saccharose was studied and compared with a reference product (refined sugar). It is part of the hypothesis that phenols bound in the test product are released into the dialysate. 

The test product Less refined sugar showed a lower amount of glucose+fructose becoming bioaccessible after brush border treatment in the dialysate, compared to the reference test product. The amount of sucrose in the dialysate fraction was comparable between the two test products indicating a difference with Less refined sugar on the sucrose digestion with brush border enzymes, possibly via phenols. 

The digestion resistant portion of less refined sugar is expected to proceed to the colon, where they would have a prebiotic effect, in particular increasing Prevotella spp. that are associated with weight loss. 

## Example 8 

The prebiotic effect of bagasse was tested using the Triskelion TNO Intestinal Model 2 . Two concentrations of Sugarcane bagasse (3.5% and 7%) were compared to a negative cellulose control. 

The effect of the test product on ammonia was assessed. In addition, microbial composition can be assessed by 16S rDNA amplicon sequencing (V4 region). Assessment of these parameters, i.e. growth and activity of (human) colonic bacteria, was done using an in vitro model which simulates colon conditions and more importantly which is predictive for the human situation. 

## Test products 

## Test product # 1 

•Name: Sugar cane Bagasse 

•Description: Composed of hemicellulose (~20-25%), cellulose (~45-55%), 

## Lignin (-1 8-24%) 

•Batch number: Bagasse Nl 201 8 

•Form: Brown powder 

• Storage conditions: ambient temperature (15-25°C),dry condition 

•Expiry date: February 201 9 

