After pre-treatment of the tiny-TIM samples with the brush border enzyme mix, all samples were analyzed on free glucose and fructose using the glucose-fructose assay (R-Biopharm). 

Obtained glucose and fructose concentrations were corrected for the dialyzed volume from the tiny-TIM system to obtain the absolute amount of glucose and fructose per sampling time point. 

## Sucrose analysis before BB treatment 

Untreated dialysate samples were analyzed on sucrose content. Samples are dissolved in diluted hydrochloric acid, and after stirring for 1 h in order to dissolve the sugars. In the resulting solution the content of the sugars were analyzed using high performance anion exchange chromatography (HPAEC). 

## Calculation 

The bioaccessible carbohydrate fraction was calculated by multiplying the total dialysate volume (V) with the analyzed glucose+fructose concentration (C) (Equation 1). Equation 1: Bioaccessible fraction [mg] = C[mglmL] V [mL] The results of the duplicate tiny-TIM runs are presented as average – sd. 

## Results 

The bioaccessible amount of sucrose and glucose+fructose was measured during and after the tiny-TIM experiments. While bioaccessibility (fraction which is potentially available for small intestinal absorption in vivo) of sucrose was measured in the dialysate samples before brush border enzyme treatment, bioaccessibility of glucose+fructose was measured after brush border enzyme treatment. The difference between the two measurements can give insight in mechanism underlying the glucose handling in the intestine for the test products. 

## Bioaccessibilitv of sucrose 

Two test products were studied using tiny-TIMcarbo technology. Less refined sugar and white refined cane sugar (reference). 

Figure 6 shows the amount of sucrose measured in the dialysate (=bioaccessible fraction) collected at the end of each experiment and before brush border treatment. Similar amounts of sucrose were taken up in the dialysate for both test products (6891 .8 – 70.6 mg refined cane sugar; 6938.3 – 186.4 mg for Less refined sugar, average – sd). 

