about 2 mM to about 6 mM, about 2 mM to about 4 mM, about 4 mM to about 10 mM, about 4 mM to about 8 mM, about 4 mM to about 6 mM, about 6 mM to about 10 mM, about 6 mM to about 8 mM, or about 8 mM to about 10 mM); beta-hyrdoxybutyrate at a final concentration of about 0.01 mM to about 0.3 mM ( e.g., about 0.01 mM to about 0.2 mM, about 0.01 mM to about 0.1 mM, about 0.01 mM to about 0.05 mM, about 0.05 mM to about 3 mM, about 0.05 mM to about 0.2 mM, about 0.05 mM to about 0.1 mM, about 0.1 mM to about 3 mM, about 0.1 mM to about 0.2 mM, or about 0.2 mM to about 0.3 mM); copper ion at a concentration of about 0.1 µM to about 1.0 µM ( e.g., about 0.1 µM to about 0.8 µM, about 0.1 µM to about 0.6 µM, about 0.1 µM to about 0.4 µM, or about 0.1 µM to about 0.2 µM), zinc ion at a final concentration of about 0.1 µM to about 10 µM (e.g., about 0.1 µM to about 8 µM, about 0.1 µM to about 6 µM, about 0.1 µM to about 4 µM, or about 0.1 µM to about 2 µM); selenium ion at a final concentration of about 0.01 µM to about 0.2 µM ( e.g., about 0.01 µM to about 0.15 µM, about 0.01 µM to about 0.10 µM, or about 0.01 µM to about 0.5 µM), iron ion at a final concentration of about 0.01 µM to about 8 µM (e.g., about 0.01 µM to about 6 µM, about 0.01 µM to about 4 µM, about 0.01 µM to about 2 µM, about 0.01 µM to about 1 µM, about 0.01 µM to about 0.05 µM), ferritin at a final concentration of about 0.1 µM to about 50 µM (e.g., 0.1 µM to about 40 µM, about 0.1 µM to about 30 µM, about 0.1 µM to about 20 µM, about 0.1 µM to about 10 µM, about 0.1 µM to about 5 µM, about 0.1 µM to about 1 µM); and transferrin at final concentration of about 0.01 µM to about 0.2 µM ( e.g., about 0.01 µM to about 0.15 µM, about 0.01 µM to about 0.10 µM, about 0.01 µM to about 0.05 µM). 

[0031] In one embodiment, the formulation is iso-osmolar relative to the vitreous. 

Formulations Free of Buffer and Organic Co-Solvent 

[0032] In another embodiment, the invention provides an aqueous formulation comprising aflibercept and a stabilizer, wherein the formulation is free of both an organic co-solvent and a buffer. 

[0033] In one embodiment, the formulation has a pH of about 5.5 to about 7.0 (e.g., or any of the subranges of this range described herein, e.g., about 5.5, about 5.6, about 5.7, about 5.8, about 5.9, about 6.0, about 6.1, about 6.2, about 6.3, about 6.4, about 6.5, about 6.6, about 6.7, about 6.8, about 6.9, or about 7.0). In another embodiment, the pH is about 6.0 to about 6.5. In yet another embodiment, the pH is about 6.2. 

[0034] In one embodiment, the stabilizer is selected from the group consisting of amino acids, sugars (e.g., sucrose), polyols, polymers, block copolymers, alkyl saccharides (e.g., a ProTek alkyl saccharides, octyl glucoside, N-decyl› pyranoside, N-octyl-glucopyranoside, Intravailfi, and those described in U.S. Pat. Nos. 9,446,134; 8,846,044, 8,226,949; 7,998,927; 8,084,022; 7,425,542; and 10,046,025), and FMl 000, or any combination thereof. 

[0035] In one embodiment, the stabilizer is a block copo› lymer. In another embodiment, the block copolymer is poloxamer (a water-soluble nonionic A-B-A and B-A-B triblock copolymers, where A is poly( ethylene oxide) (PEO) and Bis poly(propylene oxide) (PPO)). In a further embodi› ment, the poloxamer is one described in Bodratti A M, Alexandridis P. Formulation of Poloxamers for Drug Deliv› ery. J Funct Bio mater. 2018; 9(1 ): 11. 

[0036] In one embodiment, the stabilizer is a sugar. In another embodiment, the stabilizer is sucrose. 

[0037] In one embodiment, the stabilizer is an amino acid ( e.g., an aromatic amino acid, e.g., tyrosine, phenylalanine, and tryptophan). In another embodiment, the amino acid is selected from the group consisting of glycine, arginine, and praline or combinations thereof. The amino acids may be present at a concentration of about 5-300 mM ( e.g., about 25 mM to about 280 mM, about 25 mM to about 260 mM, about 25 mM to about 240 mM, about 25 mM to about 220 mM, about 25 mM to about 200 mM, about 25 mM to about 180 mM, about 25 mM to about 160 mM, about 25 mM to about 140 mM, about 25 mM to about 120 mM, about 25 mM to about 100 mM, about 25 mM to about 95 mM, about 25 mM to about 90 mM, about 25 mM to about 85 mM, about 25 mM to about 80 mM, about 25 mM to about 75 mM, about 25 mM to about 70 mM, about 25 mM to about 65 mM, about 25 mM to about 60 mM, about 25 mM to about 55 mM, about 25 mM to about 50 mM, about 25 mM to about 45 mM, about 25 mM to about 40 mM, about 25 mM to about 35 mM, about 25 mM to about 30 mM, about 30 mM to about 300 mM, about 30 mM to about 280 mM, about 30 mM to about 260 mM, about 30 mM to about 240 mM, about 30 mM to about 220 mM, about 30 mM to about 200 mM, about 30 mM to about 180 mM, about 30 mM to about 160 mM, about 30 mM to about 140 mM, about 30 mM to about 120 mM, about 30 mM to about 100 mM, about 30 mM to about 95 mM, about 30 mM to about 90 mM, about 30 mM to about 85 mM, about 30 mM to about 80 mM, about 30 mM to about 75 mM, about 30 mM to about 70 mM, about 30 mM to about 65 mM, about 30 mM to about 60 mM, about 30 mM to about 55 mM, about 30 mM to about 50 mM, about 30 mM to about 45 mM, about 30 mM to about 40 mM, about 30 mM to about 35 mM, about 35 mM to about 300 mM, about 35 mM to about 280 mM, about 35 mM to about 260 mM, about 35 mM to about 240 mM, about 35 mM to about 220 mM, about 35 mM to about 200 mM, about 35 mM to about 180 mM, about 35 mM to about 160 mM, about 35 mM to about 140 mM, about 35 mM to about 120 mM, about 35 mM to about 100 mM, about 35 mM to about 95 mM, about 35 mM to about 90 mM, about 35 mM to about 85 mM, about 35 mM to about 80 mM, about 35 mM to about 75 mM, about 35 mM to about 70 mM, about 35 mM to about 65 mM, about 35 mM to about 60 mM, about 35 mM to about 55 mM, about 35 mM to about 50 mM, about 35 mM to about 45 mM, about 35 mM to about 40 mM, about 40 mM to about 300 mM, about 40 mM to about 280 mM, about 40 mM to about 260 mM, about 40 mM to about 240 mM, about 40 mM to about 220 mM, about 40 mM to about 200 mM, about 40 mM to about 180 mM, about 40 mM to about 160 mM, about 40 mM to about 140 mM, about 40 mM to about 120 mM, about 40 mM to about 100 mM, about 40 mM to about 95 mM, about 40 mM to about 90 mM, about 40 mM to about 85 mM, about 40 mM to about 80 mM, about 40 mM to about 75 mM, about 40 mM to about 70 mM, about 40 mM to about 65 mM, about 40 mM to about 60 mM, about 40 mM to about 55 mM, about 40 mM to about 50 mM, about 40 mM to about 45 mM, about 45 mM to about 300 mM, about 45 mM to about 280 mM, about 45 mM to about 260 mM, about 45 mM to about 240 mM, about 45 mM to about 220 mM, about 45 mM to about 200 mM, about 45 mM to about 180 mM, about 45 mM to about 160 mM, about 45 mM to about 140 mM, about 45 mM to about 120 

