Human skin fibroblasts were cultured in DMEM medium (Dulbecco's modified Eagle's medium, Gibco) supplemented with 10% (v/v) fetal calf serum (FCS), 2 mM l-glutamine, 50 μg/ml uridine, 110 μg/ml pyruvate, 10,000 U/ml penicillin G and 10,000 μg/ml streptomycin.
DMEM medium
fetal calf serum (FCS)
10%
DMEM medium
l-glutamine
2 mM
mM
DMEM medium
50 μg/ml
μg/ml
DMEM medium
pyruvate
110 μg/ml
μg/ml
DMEM medium
penicillin G
10,000 U/ml
U/ml
DMEM medium
streptomycin
10,000 μg/ml
μg/ml