After various experiments on E. coli, yeast, and fruit fly cells, they still could not produce the enzyme with the carbohydrate attached. Finally, they succeeded in isolating enzyme with the bound carbohydrate from a nutrient medium in which hamster cells had been cultivated. After purification, the enzyme could be injected into the body and penetrate skeletal muscle and heart tissue cells, enabling the breakdown of glycogen in infants with Pompe disease.
經過大腸菌、酵母菌及果蠅細胞等各種實驗，都沒有辦法製造出帶有基因標誌的酵素，最後才在倉鼠細胞的培養液中，成功分離出帶有基因標誌的酵素，經過純化，注入體內可以通行無阻地到達肌肉與心臟細胞，在「龐貝氏症」病童身上發揮了分解肝醣的功效。