After squeezing the rubber bladder, the suction tube must be placed right up next to the larva without actually pressing down on it. When the hand over the bladder is relaxed, the larva is sucked into the tube. If the operator is clumsy, and touches the larva, it will be injured if not killed, affecting the collection effort and leaving the experts feeling a little sad.
這整套採集孑孓的工作看來不算複雜，可是得輕手輕腳地，先捏住橡皮頭，然後在吸管與孑孓將觸未觸之際，手一鬆，吸進來；若是笨手笨腳的，觸到幼蟲身體，非死即傷，影響了採集成果，老師傅可是會心疼的。