ACT_SITE 72; /note="Schiff-base intermediate with substrate; via pyruvic acid"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 86; /note="Proton donor; for catalytic activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 241; /note="Proton acceptor; for processing activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 254; /note="Proton acceptor; for processing activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"