ACT_SITE 71; /note="Schiff-base intermediate with substrate; via pyruvic acid"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 85; /note="Proton donor; for catalytic activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 225; /note="Proton acceptor; for processing activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"; ACT_SITE 238; /note="Proton acceptor; for processing activity"; /evidence="ECO:0000256|PIRSR:PIRSR001355-1"