My Little Pony：友情は魔法です。そこで、彼女はさまざまな性格を持つ他の5人のポニーと友達になり、彼らの助けを借りてさまざまな問題を解決し始めます。
My Little Pony: Friendship is Magic follows the life of Twilight Sparkle, a book-smart pony who is sent to Ponyville to learn about friendship. There, she befriends five other ponies with varying personalities, and starts solving various problems with their help.