  The genome-scale metabolic model with protein constraint (PC-model) has been
increasingly popular for microbial metabolic simulations. We present PROSO
Toolbox, a unified and simple-to-use PC-model toolbox that takes any
high-quality genome-scale metabolic reconstruction as the input. The toolbox
can construct a PC-model automatically, apply various algorithms for
computational strain design and simulation, and help unveil metabolism from
gene expression data through a state-of-the-art OVERLAY workflow. It also has
detailed tutorials and documentation for maximum accessibility to researchers
from diverse backgrounds. PROSO Toolbox, tutorials, and documentation are
freely available online: https://github.com/QCSB/PROSO-Toolbox.

Molecular Networks;Quantitative Methods