  The small correction volume for conventional wavefront shaping methods limits
their applications in biological imaging through scattering media. We
demonstrate large volume wavefront shaping through a scattering layer with a
single correction by conjugate adaptive optics and remote focusing (CAORF). The
remote focusing module can keep the conjugation between the AO and scattering
layer during three-dimensional scanning. This new configuration provides a
wider correction volume by the best utilization of the memory effect in a fast
three-dimensional laser scanning microscope. Our results show that the proposed
system can provide 10 times wider axial field of view compared with a
conventional conjugate AO system when 16,384 segments are used on a spatial
light modulator. We also demonstrated three-dimensional fluorescence imaging
and multi-spot patterning through a scattering layer.

Biological Physics;Optics