  Calcium imaging using miniscopes are becoming increasingly popular in the
neuroscience community with a multitude of microendoscope versions, lens
systems and packages for analysis available as open source. Here we describe in
detail how to implant lenses and baseplates for the University of California at
Los Angeles (UCLA) miniscope V3 and V4 using a Gradient Index (GRIN) lens for
the hippocampus or a combination of prism and GRIN lens for recording in the
neocortex. Our protocols contain adaptations from several published protocols
however the GRIN-relay-Prism lens system protocol is developed in-house and not
previously described. This chapter also suggests how lenses may be re-used and
how to test the quality of a reused lens. Lastly, we comment on the difference
between analysis software and the computational needs for different packages.

Neurons and Cognition