  We report on wide-field imaging of pulsatile microvascular blood flow in the
exposed cerebral cortex of a mouse by holographic interferometry. We recorded
interferograms of laser light backscattered by the tissue, beating against an
off-axis reference beam with a 50 kHz framerate camera. Videos of local Doppler
contrasts were rendered numerically by Fresnel transformation and short-time
Fourier transform analysis. This approach enabled instantaneous imaging of
pulsatile blood flow contrasts in superficial blood vessels over 256 x 256
pixels with a spatial resolution of 10 microns and a temporal resolution of 20
ms.

Medical Physics;Optics