  We developed pgMAP, an analysis pipeline to map gRNA sequencing reads from
dual-targeting CRISPR screens. pgMAP output includes a dual gRNA read counts
table and quality control metrics including the proportion of correctly-paired
reads and CRISPR library sequencing coverage across all time points and
samples. pgMAP is implemented using Snakemake and is available open-source
under the MIT license at https://github.com/fredhutch/pgmap_pipeline.

Genomics