  Three-dimensional Polarized Light Imaging (3D-PLI) is a promising technique
to reconstruct the nerve fiber architecture of human post-mortem brains from
birefringence measurements of histological brain sections with micrometer
resolution. To better understand how the reconstructed fiber orientations are
related to the underlying fiber structure, numerical simulations are employed.
Here, we present two complementary simulation approaches that reproduce the
entire 3D-PLI analysis: First, we give a short review on a simulation approach
that uses the Jones matrix calculus to model the birefringent myelin sheaths.
Afterwards, we introduce a more sophisticated simulation tool: a 3D Maxwell
solver based on a Finite-Difference Time-Domain algorithm that simulates the
propagation of the electromagnetic light wave through the brain tissue. We
demonstrate that the Maxwell solver is a valuable tool to better understand the
interaction of polarized light with brain tissue and to enhance the accuracy of
the fiber orientations extracted by 3D-PLI.

Medical Physics;Optics