  Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of
fluorescence lifetime measurements is usually limited in speed due to pile-up.
With modern instrumentation this limitation can be lifted significantly but
some artefacts due to frequent merging of closely spaced detector pulses
(detector pulse pile-up) remains an issue to be addressed. We propose here a
data analysis method correcting for this type of artefact and the resulting
systematic errors. It physically models the photon losses due to detector pulse
pile-up and incorporates the loss in the decay fit model employed to obtain
fluorescence lifetimes and relative amplitudes of the decay components.
Comparison of results with and without this correction show a significant
reduction of systematic errors at count rates approaching the excitation rate.
This allows quantitatively accurate fluorescense lifetime imaging (FLIM) at
very high frame rates.

Data Analysis, Statistics and Probability;Instrumentation and Detectors;Optics