6.1: Ultraviolet-visible spectroscopy (UV-vis) 6.1.1: Methods of Calibration 6.1.2: Data analysis 6.1.3: Pre-lab assignment 6.1.4: Absorbance and Beer's Law 6.1.5: UV/Vis Instrumentation 6.1.6: UV-vis multicomponent analysis procedure 6.1.7: Data analysis 6.2: Cyclic Voltammetry Cyclic voltammetry (CV) is a technique used to study electrochemical reaction mechanisms that give rise to electroanalytical current signals. The method involves linearly varying an electrode potential between two limits at a specific rate while monitoring the current that develops in an electrochemical cell under conditions where voltage is in excess of that predicted by the Nernst equation.¹ 6.3: Fourier Transform Infrared Spectroscopy (FTIR) The FTIR / Vibrational spectroscopy exercise follows the format of a detective story involving solving a series of problems rather than the normal lab format. The experiment is an adaptation of "Pollution Police" by Profs. Jodye Selco and Janet Beery at the University of Redlands, which was presented at the Division of Chemical Education Regional ACS meeting in Ontario, CA 1999. 6.4: Molecular Fluorescence When a molecule absorbs a photon in the ultraviolet or visible (UV VIS) region (180 - 780 nm), an electronic transition occurs within the molecule. This transition involves moving an electron from the singlet ground state to a singlet excited state. 6.5: Capillary Electrophoresis Electrophoresis is a class of separation techniques in which we separate analytes by their ability to move through a conductive medium—usually an aqueous buffer—in response to an applied electric field. In the absence of other effects, cations migrate toward the electric field’s negatively charged cathode. 6.6: Electrospray Mass Spectrometry In this experiment you will learn the mechanics of electrospray mass spectrometry and use this technique to study the effect of different solvents on the three-dimensional structure of proteins. 6.7: Vocabulary 6.8: GC-MS Procedure and instructions for data analysis This lab involves the analysis of different unknown mixtures by high resolution capillary gas chromatography (GC) with an ion trap detector (ITD). The ITD is a variation of a quadrupole mass spectrometer and is designed to function specifically as a GC detector. Due to the design variances of the ITD compared to a true quadrupole mass spectrometer, the ITD mass spectrum of an organic compound may not be identical (but should be very similar) to its classical electron impact (EI) mass spectrum. 6.9: Instructions for Manual Operation of the Agilent GCMS 6.10: Caffeine serial dilution and analysis using (UV-vis) spectroscopy 6.10.1: Pre-lab assignment 6.10.2: TA Notes