133
Heterozygote detection was performed with an allele - specific polymerase chain reaction ( PCR ) system designed to detect exclusively only one of the normal and mutant alleles , followed by confirmation with PCR / single - strand conformation polymorphism ( SSCP ) analysis and direct sequencing .
 None
Given options, please tell me the categories of all the listed entity words.Output format is "type1: word1; type2: word2". 
Option: Disease 
Text: {0} 
 Entities:  
Answer:
 None