12402
Following stimulation, TNF-alpha was measured in cell supernatants by ELISA , NF-kappaB activity by electrophoretic mobility shift assay , and IkappaB-alpha levels by Western blot .
 ( protein: TNF-alpha, None) , ( protein: IkappaB-alpha, None) , ( protein: NF-kappaB, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF-alpha, None) , ( protein: IkappaB-alpha, None) , ( protein: NF-kappaB, None) 