3486
We overexpressed in the Jurkat T-cell line ZAP-70 mutated on Tyr-492 and Tyr-493 in the putative regulatory loop of its kinase domain .
 ( protein: kinase domain, None) , ( protein: ZAP-70, None) , ( cell line: Jurkat T-cell line, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: kinase domain, None) , ( protein: ZAP-70, None) , ( cell line: Jurkat T-cell line, None) 