7564
Transient transfection of endothelial cells with ICAM-1 promoter luciferase reporter gene (ICAM-1LUC) constructs indicated that deletion of upstream NF-kappa B site ( -533 bases from translation start site ) had no effect on thrombin responsiveness , whereas mutation/deletion of downstream NF-kappa B site ( -223 bases from the translation start site ) prevented the activation of ICAM-1 promoter , indicating that the downstream NF-kappa B site is critical for thrombin inducibility .
 ( protein: NF-kappa B, None) , ( DNA: -533 bases, None) , ( DNA: translation start site, None) , ( protein: NF-kappa B, None) , ( DNA: ICAM-1 promoter luciferase reporter gene (ICAM-1LUC) constructs, None) , ( DNA: -223 bases, None) , ( DNA: ICAM-1 promoter, None) , ( protein: ICAM-1, None) , ( DNA: translation start site, None) , ( DNA: NF-kappa B site, None) , ( protein: ICAM-1, None) , ( DNA: ICAM-1 promoter, None) , ( DNA: NF-kappa B site, None) , ( cell type: endothelial cells, None) , ( protein: NF-kappa B, None) , ( DNA: NF-kappa B site, None) , ( protein: thrombin, None) , ( protein: thrombin, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: NF-kappa B, None) , ( DNA: -533 bases, None) , ( DNA: translation start site, None) , ( protein: NF-kappa B, None) , ( DNA: ICAM-1 promoter luciferase reporter gene (ICAM-1LUC) constructs, None) , ( DNA: -223 bases, None) , ( DNA: ICAM-1 promoter, None) , ( protein: ICAM-1, None) , ( DNA: translation start site, None) , ( DNA: NF-kappa B site, None) , ( protein: ICAM-1, None) , ( DNA: ICAM-1 promoter, None) , ( DNA: NF-kappa B site, None) , ( cell type: endothelial cells, None) , ( protein: NF-kappa B, None) , ( DNA: NF-kappa B site, None) , ( protein: thrombin, None) , ( protein: thrombin, None) 