14218
To determine whether Nef affects the binding of cellular factors implicated in HIV-1 regulation , 32P-labeled oligonucleotides corresponding to the binding sites were incubated with nuclear extracts prepared from Nef-expressing T-cell lines that were not stimulated or were stimulated with T-cell mitogens .
 ( cell line: Nef-expressing T-cell lines, None) , ( protein: cellular factors, None) , ( protein: Nef, None) , ( protein: T-cell mitogens, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: Nef-expressing T-cell lines, None) , ( protein: cellular factors, None) , ( protein: Nef, None) , ( protein: T-cell mitogens, None) 