5290
mRNA expression was identified after RNA extraction using RNAzol and analysed after reverse transcriptase , by polymerase chain reaction using a semiquantitative competitive hybridization assay .
 ( protein: reverse transcriptase, None) , ( RNA: mRNA, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: reverse transcriptase, None) , ( RNA: mRNA, None) 