4573
To investigate the molecular mechanism of the inhibitory effect on transcriptional or post-transcriptional regulation of IL-6 gene expression by IL-4 and IL-10 , we studied IL-6 production, expression level of IL-6 mRNA , IL-6 promoter activity , transcriptional activity of NF-kappaB and NF- IL-6 , and IL-6 mRNA stability in human monocytic cell lines , THP-1 and U937 , stimulated by PMA and LPS in the absence or the presence of IL-4 or IL-10 .
 ( RNA: IL-6 mRNA, None) , ( protein: IL-10, None) , ( protein: NF-kappaB, None) , ( cell line: human monocytic cell lines, None) , ( protein: IL-6, None) , ( DNA: IL-6 gene, None) , ( protein: IL-6, None) , ( protein: IL-6, None) , ( protein: IL-4, None) , ( protein: IL-6, None) , ( protein: IL-4, None) , ( protein: NF- IL-6, None) , ( cell line: U937, None) , ( protein: IL-6, None) , ( RNA: IL-6 mRNA, None) , ( cell line: THP-1, None) , ( protein: IL-10, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( RNA: IL-6 mRNA, None) , ( protein: IL-10, None) , ( protein: NF-kappaB, None) , ( cell line: human monocytic cell lines, None) , ( protein: IL-6, None) , ( DNA: IL-6 gene, None) , ( protein: IL-6, None) , ( protein: IL-6, None) , ( protein: IL-4, None) , ( protein: IL-6, None) , ( protein: IL-4, None) , ( protein: NF- IL-6, None) , ( cell line: U937, None) , ( protein: IL-6, None) , ( RNA: IL-6 mRNA, None) , ( cell line: THP-1, None) , ( protein: IL-10, None) 