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As NF-kappa B is a critical transcription factor in the control of IL-2 expression , we next analysed its nuclear translocation in neonatal and adult T cells using the electrophoretic mobility shift assay and, because induction of reactive oxygen intermediates ( ROI ) is required for the activation of NF-kappa B , we also analysed levels of intracellular ROI in these cells using the ROI -reactive fluorochrome DCFH-DA and flow cytometry .
 ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: NF-kappa B, None) , ( protein: IL-2, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: NF-kappa B, None) , ( protein: transcription factor, None) , ( protein: NF-kappa B, None) , ( protein: IL-2, None) 