6262
Although the TNF-alpha level in culture supernatants was below the sensitivity of an ELISA assay system , addition of anti- TNF-alpha antibody in culture medium could partially suppress the heat shock induced HIV-1 production .
 ( protein: TNF-alpha, None) , ( protein: TNF-alpha, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF-alpha, None) , ( protein: TNF-alpha, None) 