6962
We found that overexpression of IkappaB-alpha in endothelial cells using a recombinant adenovirus prevented tumor necrosis factor-alpha ( TNF-alpha )-induced degradation of IkappaB-alpha and suppressed the upregulation of vascular cell adhesion molecule-1 ( VCAM-1 ), intercellular adhesion molecule-1 ( ICAM-1 ), and E-selectin mRNA and surface protein expression and the upregulation of transcripts for the chemokines monocyte chemoattractant protein 1 ( MCP-1 ) and growth-related activity-alpha ( GRO-alpha ) by TNF-alpha .
 ( protein: GRO-alpha, None) , ( protein: TNF-alpha, None) , ( protein: ICAM-1, None) , ( protein: monocyte chemoattractant protein 1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: endothelial cells, None) , ( protein: chemokines, None) , ( protein: IkappaB-alpha, None) , ( RNA: E-selectin mRNA, None) , ( protein: IkappaB-alpha, None) , ( protein: MCP-1, None) , ( protein: E-selectin, None) , ( protein: growth-related activity-alpha, None) , ( protein: VCAM-1, None) , ( protein: intercellular adhesion molecule-1, None) , ( protein: TNF-alpha, None) , ( protein: vascular cell adhesion molecule-1, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: GRO-alpha, None) , ( protein: TNF-alpha, None) , ( protein: ICAM-1, None) , ( protein: monocyte chemoattractant protein 1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: endothelial cells, None) , ( protein: chemokines, None) , ( protein: IkappaB-alpha, None) , ( RNA: E-selectin mRNA, None) , ( protein: IkappaB-alpha, None) , ( protein: MCP-1, None) , ( protein: E-selectin, None) , ( protein: growth-related activity-alpha, None) , ( protein: VCAM-1, None) , ( protein: intercellular adhesion molecule-1, None) , ( protein: TNF-alpha, None) , ( protein: vascular cell adhesion molecule-1, None) 