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To clarify the presence of histamine receptor and its transmembrane mechanism in human T lymphocytes , we investigated the effects of agonists or antagonists of histamine receptor subtypes and bacterial toxins on intracellular concentration of Ca2+ [ Ca2+ ]i ), [3H]pyrilamine binding and c-fos mRNA expression in Jurkat cells , cloned human T lymphocytes .
 ( cell line: Jurkat cells, None) , ( protein: histamine receptor, None) , ( protein: histamine receptor, None) , ( RNA: c-fos mRNA, None) , ( cell type: human T lymphocytes, None) , ( cell type: human T lymphocytes, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: Jurkat cells, None) , ( protein: histamine receptor, None) , ( protein: histamine receptor, None) , ( RNA: c-fos mRNA, None) , ( cell type: human T lymphocytes, None) , ( cell type: human T lymphocytes, None) 