14603
In previous transfection analyses using the chloramphenicol acetyltransferase reporter gene system , we determined that linker substitution (LS) mutations between -201 and -130 (relative to the transcription start site) of the human immunodeficiency virus type 1 long terminal repeat ( LTR ) caused moderate decreases in LTR transcriptional activity in a T-cell line (S.L.Zeichner, J.Y.H. Kim, and J.C.Alwine, J.Virol.65:2436-2444, 1991).
 ( cell line: T-cell line, None) , ( DNA: reporter gene, None) , ( DNA: LTR, None) , ( DNA: LTR, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: long terminal repeat, None) , ( DNA: -201 and -130, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: T-cell line, None) , ( DNA: reporter gene, None) , ( DNA: LTR, None) , ( DNA: LTR, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: long terminal repeat, None) , ( DNA: -201 and -130, None) 