10706
For this purpose, we transiently transfected Jurkat T cells with the reporter plasmid p[(TRE)3-tk-Luc] and found that CCK-8 was able to dose-dependently induce luciferase expression related to activator protein-1 ( AP-1 ) activation with a maximal response identical to that obtained with compounds known to activate AP-1 complex (quantitatively, the same level of induction was obtained with 1 nM 12-O-tetradecanoylphorbol-13-acetate , 100 microM diacylglycerol , or 4 nM epidermal growth factor ).
 ( protein: luciferase, None) , ( protein: activator protein-1, None) , ( protein: AP-1 complex, None) , ( protein: AP-1, None) , ( DNA: reporter plasmid p[(TRE)3-tk-Luc], None) , ( protein: AP-1, None) , ( cell line: Jurkat T cells, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: luciferase, None) , ( protein: activator protein-1, None) , ( protein: AP-1 complex, None) , ( protein: AP-1, None) , ( DNA: reporter plasmid p[(TRE)3-tk-Luc], None) , ( protein: AP-1, None) , ( cell line: Jurkat T cells, None) 