4943
The EGR1 binding motif was shown to be necessary for stimulus-induced expression of a CD44 promoter-chloramphenicol acetyltransferase reporter construct in nontransformed B lymphocytes and was required for transactivation by an EGR1 expression vector in a B-cell line .
 ( cell line: B-cell line, None) , ( DNA: EGR1 binding motif, None) , ( protein: EGR1, None) , ( DNA: EGR1 expression vector, None) , ( protein: CD44, None) , ( DNA: promoter-chloramphenicol acetyltransferase reporter construct, None) , ( protein: EGR1, None) , ( cell type: nontransformed B lymphocytes, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: B-cell line, None) , ( DNA: EGR1 binding motif, None) , ( protein: EGR1, None) , ( DNA: EGR1 expression vector, None) , ( protein: CD44, None) , ( DNA: promoter-chloramphenicol acetyltransferase reporter construct, None) , ( protein: EGR1, None) , ( cell type: nontransformed B lymphocytes, None) 