7405
We have used this DNA fragment to isolate and purify a 60-kDa protein binding to this fragment and obtained its amino-terminal sequence , which was used to design degenerate probes to screen a cDNA library from THP-1 cells .
 ( cell line: THP-1 cells, None) , ( DNA: degenerate probes, None) , ( protein: amino-terminal sequence, None) , ( protein: 60-kDa protein, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: THP-1 cells, None) , ( DNA: degenerate probes, None) , ( protein: amino-terminal sequence, None) , ( protein: 60-kDa protein, None) 