5483
In combination with TNF-alpha , IL-10 stimulated activating protein-1 ( AP-1 ) and nuclear factor (NF)-kappa B binding activities and cooperated to increase HIV-1 steady-state mRNA levels and enhance long terminal repeat -directed transcription through activation of the NF-kappa B binding sites , suggesting the IL-10 effect occurs at least in part at the transcriptional level .
 ( protein: TNF-alpha, None) , ( protein: AP-1, None) , ( protein: NF-kappa B, None) , ( DNA: long terminal repeat, None) , ( protein: activating protein-1, None) , ( protein: nuclear factor (NF)-kappa B, None) , ( protein: IL-10, None) , ( protein: IL-10, None) , ( DNA: NF-kappa B binding sites, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF-alpha, None) , ( protein: AP-1, None) , ( protein: NF-kappa B, None) , ( DNA: long terminal repeat, None) , ( protein: activating protein-1, None) , ( protein: nuclear factor (NF)-kappa B, None) , ( protein: IL-10, None) , ( protein: IL-10, None) , ( DNA: NF-kappa B binding sites, None) 