12460
In addition, both heat and cadmium produced an increase in the amount of the intermediate filament protein vimentin , as determined by immunoblot and immunofluorescence assays .
 ( protein: intermediate filament protein, None) , ( protein: vimentin, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: intermediate filament protein, None) , ( protein: vimentin, None) 