1985
The antigenic specificity of an HLA DQ2-restricted, HSV-2 type-specific T-cell clone was mapped to amino acids 425 to 444 of VP16 of HSV-2 by sequential use of an intertypic recombinant virus containing VP16 of HSV-2 in an HSV-1 background , recombinant VP16 fusion proteins , and synthetic peptides .
 ( protein: VP16, None) , ( protein: VP16, None) , ( protein: recombinant VP16 fusion proteins, None) , ( cell line: HLA DQ2-restricted, HSV-2 type-specific T-cell clone, None) , ( protein: VP16, None) , ( protein: amino acids 425 to 444, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: VP16, None) , ( protein: VP16, None) , ( protein: recombinant VP16 fusion proteins, None) , ( cell line: HLA DQ2-restricted, HSV-2 type-specific T-cell clone, None) , ( protein: VP16, None) , ( protein: amino acids 425 to 444, None) 