9534
Furthermore, deletion and mutation analyses of the VCAM-1 promoter performed with chloramphenicol acetyltransferase constructs revealed that Tax was trans activating the VCAM-1 promoter via two NF-kappaB sites present at bp -72 and -57 in the VCAM-1 gene promoter , with both of them being required for the Tax -induced expression of this adhesion molecule .
 ( protein: adhesion molecule, None) , ( protein: NF-kappaB, None) , ( protein: VCAM-1, None) , ( protein: VCAM-1, None) , ( protein: Tax, None) , ( protein: VCAM-1, None) , ( DNA: VCAM-1 gene promoter, None) , ( DNA: VCAM-1 promoter, None) , ( DNA: VCAM-1 promoter, None) , ( protein: Tax, None) , ( DNA: NF-kappaB sites, None) , ( DNA: chloramphenicol acetyltransferase constructs, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: adhesion molecule, None) , ( protein: NF-kappaB, None) , ( protein: VCAM-1, None) , ( protein: VCAM-1, None) , ( protein: Tax, None) , ( protein: VCAM-1, None) , ( DNA: VCAM-1 gene promoter, None) , ( DNA: VCAM-1 promoter, None) , ( DNA: VCAM-1 promoter, None) , ( protein: Tax, None) , ( DNA: NF-kappaB sites, None) , ( DNA: chloramphenicol acetyltransferase constructs, None) 