4959
We found that two IL-1alpha kappaB-like sites (positions -1,065 to -1,056 and +646 to +655 ) specifically formed a complex with NF-kappaB -containing nuclear extract from MT-2 cells and that NF-kappaB bound with higher affinity to the 3' NF-kappaB binding site than to the 5' NF-kappaB site .
 ( protein: NF-kappaB, None) , ( protein: NF-kappaB, None) , ( DNA: +646 to +655, None) , ( DNA: -1,065 to -1,056, None) , ( protein: NF-kappaB, None) , ( protein: NF-kappaB, None) , ( cell line: MT-2 cells, None) , ( DNA: 3#$%# NF-kappaB binding site, None) , ( protein: IL-1alpha, None) , ( DNA: 5#$%# NF-kappaB site, None) , ( DNA: IL-1alpha kappaB-like sites, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: NF-kappaB, None) , ( protein: NF-kappaB, None) , ( DNA: +646 to +655, None) , ( DNA: -1,065 to -1,056, None) , ( protein: NF-kappaB, None) , ( protein: NF-kappaB, None) , ( cell line: MT-2 cells, None) , ( DNA: 3#$%# NF-kappaB binding site, None) , ( protein: IL-1alpha, None) , ( DNA: 5#$%# NF-kappaB site, None) , ( DNA: IL-1alpha kappaB-like sites, None) 