13776
To determine the mechanism of action of Ro 09-2210 , we set up a transient expression system in Jurkat T cells using a variety of reporter gene constructs and showed effective inhibition of phorbol ester / ionomycin -induced NF-AT activation and anti-CD3 induced NF-AT with IC50 = 7.7 and 10 nM, respectively.
 ( protein: NF-AT, None) , ( cell line: Jurkat T cells, None) , ( protein: NF-AT, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: NF-AT, None) , ( cell line: Jurkat T cells, None) , ( protein: NF-AT, None) 