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By using a partial nucleotide sequence from the cloned TSC2 and polymerase chain reaction methodology , we constructed a digoxigenin-labeled complementary DNA probe to examine TSC2 gene expression in autopsy- or biopsy-derived human tissues by in situ hybridization .
 ( DNA: TSC2, None) , ( DNA: TSC2, None) , ( DNA: partial nucleotide sequence, None) , ( DNA: digoxigenin-labeled complementary DNA probe, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: TSC2, None) , ( DNA: TSC2, None) , ( DNA: partial nucleotide sequence, None) , ( DNA: digoxigenin-labeled complementary DNA probe, None) 