12823
Unlike ligation of CD3 , stimulation with anti- CD28 mAb or CHO cells expressing the CD28 ligands CD80 or CD86 did not lead to tyrosine phosphorylation of HS1 in Jurkat T cells .
 ( protein: CD28 ligands, None) , ( protein: HS1, None) , ( protein: anti- CD28 mAb, None) , ( cell line: Jurkat T cells, None) , ( protein: CD28, None) , ( protein: CD80, None) , ( protein: CD86, None) , ( protein: CD28, None) , ( protein: CD3, None) , ( cell line: CHO cells, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: CD28 ligands, None) , ( protein: HS1, None) , ( protein: anti- CD28 mAb, None) , ( cell line: Jurkat T cells, None) , ( protein: CD28, None) , ( protein: CD80, None) , ( protein: CD86, None) , ( protein: CD28, None) , ( protein: CD3, None) , ( cell line: CHO cells, None) 