14505
Using transfection and reporter gene assays specifically designed for primary T lymphocytes in conjunction with gel retardation assays , Western blot analyses and UV cross-linking studies , we found that c-Jun , c-Fos , and octamer-binding proteins play a major role in transcriptional activation of the IL3 gene via their interaction with two specific regions contained within the IL3 5'-flanking sequence .
 ( protein: c-Jun, None) , ( protein: IL3, None) , ( protein: IL3, None) , ( protein: octamer-binding proteins, None) , ( DNA: IL3 gene, None) , ( DNA: IL3 5#$%#-flanking sequence, None) , ( protein: c-Fos, None) , ( cell type: primary T lymphocytes, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: c-Jun, None) , ( protein: IL3, None) , ( protein: IL3, None) , ( protein: octamer-binding proteins, None) , ( DNA: IL3 gene, None) , ( DNA: IL3 5#$%#-flanking sequence, None) , ( protein: c-Fos, None) , ( cell type: primary T lymphocytes, None) 