2062
Because the Nef protein does not affect the surface expression of the CD3 -TCR complex, we conclude that the Nef protein down-regulates the transcriptional factors NF-kappa B and AP-1 in T cells in vitro through an effect on the TCR-dependent signal transduction pathway .
 ( protein: AP-1, None) , ( cell type: T cells, None) , ( protein: NF-kappa B, None) , ( protein: CD3, None) , ( protein: Nef, None) , ( protein: Nef, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: AP-1, None) , ( cell type: T cells, None) , ( protein: NF-kappa B, None) , ( protein: CD3, None) , ( protein: Nef, None) , ( protein: Nef, None) 