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The role of the human immunodeficiency virus ( HIV-1 ) Nef protein in T cell activation pathways was investigated using a Jurkat CD4 + cell line stably transfected with a Nef expression vector .
 ( cell line: Jurkat CD4 + cell line, None) , ( protein: human immunodeficiency virus ( HIV-1 ) Nef protein, None) , ( protein: Nef protein, None) , ( protein: CD4, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: Jurkat CD4 + cell line, None) , ( protein: human immunodeficiency virus ( HIV-1 ) Nef protein, None) , ( protein: Nef protein, None) , ( protein: CD4, None) 