7337
When isolated PBMC were first cultured for 20 h in the presence of IL-10 on Teflon to prevent adherence, washed to remove IL-10 and then further cultured in plastic dishes for an additional 20 h in the presence of LPS or IL-1beta , an enhanced release of TNF was observed.
 ( protein: IL-10, None) , ( protein: IL-1beta, None) , ( protein: TNF, None) , ( protein: IL-10, None) , ( cell type: PBMC, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: IL-10, None) , ( protein: IL-1beta, None) , ( protein: TNF, None) , ( protein: IL-10, None) , ( cell type: PBMC, None) 