2055
The presence of TN in culture medium does not detectably alter the pattern of tyrosine phosphorylation resulting from T cell triggering with alpha CD3 , but at later time points prevents the appearance of functional NF-AT1 transcription factor complexes in T cell nuclear extracts .
 ( protein: TN, None) , ( protein: T cell nuclear extracts, None) , ( protein: CD3, None) , ( protein: alpha CD3, None) , ( protein: NF-AT1 transcription factor complexes, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TN, None) , ( protein: T cell nuclear extracts, None) , ( protein: CD3, None) , ( protein: alpha CD3, None) , ( protein: NF-AT1 transcription factor complexes, None) 