475
We show in this work that mutations in either the purine -rich region of the CLEO element or the NF-kappa B site result in reduced PMA / Ca2+ activation of a 620-bp human GM-CSF promoter- luciferase reporter construct in Jurkat T cells by 65% and 50%, respectively.
 ( protein: GM-CSF, None) , ( protein: NF-kappa B, None) , ( protein: luciferase, None) , ( DNA: CLEO, None) , ( DNA: purine -rich region, None) , ( DNA: CLEO element, None) , ( DNA: NF-kappa B site, None) , ( cell line: Jurkat T cells, None) , ( cell type: T cells, None) , ( DNA: 620-bp human GM-CSF promoter- luciferase reporter construct, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: GM-CSF, None) , ( protein: NF-kappa B, None) , ( protein: luciferase, None) , ( DNA: CLEO, None) , ( DNA: purine -rich region, None) , ( DNA: CLEO element, None) , ( DNA: NF-kappa B site, None) , ( cell line: Jurkat T cells, None) , ( cell type: T cells, None) , ( DNA: 620-bp human GM-CSF promoter- luciferase reporter construct, None) 