526
Adhesion of human monocytes to P-selectin , the most rapidly expressed endothelial tethering factor , increased the secretion of monocyte chemotactic protein-1 ( MCP-1 ) and tumor necrosis factor-alpha ( TNF-alpha ) by the leukocytes when they were stimulated with platelet-activating factor .
 ( protein: MCP-1, None) , ( protein: platelet-activating factor, None) , ( protein: P-selectin, None) , ( cell type: human monocytes, None) , ( protein: TNF-alpha, None) , ( cell type: leukocytes, None) , ( protein: monocyte chemotactic protein-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: monocytes, None) , ( protein: endothelial tethering factor, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: MCP-1, None) , ( protein: platelet-activating factor, None) , ( protein: P-selectin, None) , ( cell type: human monocytes, None) , ( protein: TNF-alpha, None) , ( cell type: leukocytes, None) , ( protein: monocyte chemotactic protein-1, None) , ( protein: tumor necrosis factor-alpha, None) , ( cell type: monocytes, None) , ( protein: endothelial tethering factor, None) 