10624
Functional analysis of monocytic THP-1 cells transfected with plasmids containing various lengths of TNF-alpha promoter localized enhancer elements in a region ( -182 to -37 base pairs (bp)) that were required for optimal transcription of the TNF-alpha gene in response to LPS .
 ( DNA: plasmids, None) , ( DNA: TNF-alpha promoter, None) , ( cell line: monocytic THP-1 cells, None) , ( DNA: TNF-alpha gene, None) , ( DNA: -182 to -37 base pairs, None) , ( cell line: THP-1 cells, None) , ( DNA: enhancer elements, None) , ( protein: TNF-alpha, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: plasmids, None) , ( DNA: TNF-alpha promoter, None) , ( cell line: monocytic THP-1 cells, None) , ( DNA: TNF-alpha gene, None) , ( DNA: -182 to -37 base pairs, None) , ( cell line: THP-1 cells, None) , ( DNA: enhancer elements, None) , ( protein: TNF-alpha, None) 