3657
To determine whether the functional defect of the patients' T cells was due to the absence or abnormal binding of transcription factors involved in cytokine gene expression , electrophoretic mobility shift assays were used to examine the DNA binding of AP-1 , Oct , CREB , SP1 , NF-kappa B and the nuclear factor of activated T cells ( NF-AT ) to their respective response elements in the promoter of the IL-2 gene .
 ( DNA: IL-2 gene, None) , ( protein: Oct, None) , ( protein: SP1, None) , ( protein: AP-1, None) , ( DNA: promoter, None) , ( DNA: response elements, None) , ( protein: NF-AT, None) , ( protein: IL-2, None) , ( cell type: T cells, None) , ( protein: transcription factors, None) , ( cell type: T cells, None) , ( DNA: cytokine gene, None) , ( protein: CREB, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor of activated T cells, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: IL-2 gene, None) , ( protein: Oct, None) , ( protein: SP1, None) , ( protein: AP-1, None) , ( DNA: promoter, None) , ( DNA: response elements, None) , ( protein: NF-AT, None) , ( protein: IL-2, None) , ( cell type: T cells, None) , ( protein: transcription factors, None) , ( cell type: T cells, None) , ( DNA: cytokine gene, None) , ( protein: CREB, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor of activated T cells, None) 