13719
The effect of Rho on AP-1 is independent of the mitogen-activated protein kinase pathway , as a dominant-negative MEK and a MEK inhibitor ( PD98059 ) did not affect Rho -induced AP-1 activity .
 ( protein: Rho, None) , ( protein: AP-1, None) , ( protein: MEK, None) , ( protein: Rho, None) , ( protein: MEK, None) , ( protein: mitogen-activated protein kinase, None) , ( protein: AP-1, None) , ( protein: dominant-negative MEK, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: Rho, None) , ( protein: AP-1, None) , ( protein: MEK, None) , ( protein: Rho, None) , ( protein: MEK, None) , ( protein: mitogen-activated protein kinase, None) , ( protein: AP-1, None) , ( protein: dominant-negative MEK, None) 