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To get further insights into the molecular mechanisms underlying the biological activity of Tat , we investigated the effect of endogenous and exogenous Tat protein on c-fos gene expression in T lymphoblastoid (Jurkat) and monocytic (U937) cell lines , as well as in primary peripheral blood mononuclear cells ( PBMC ).
 ( protein: Tat, None) , ( cell type: primary peripheral blood mononuclear cells, None) , ( protein: Tat protein, None) , ( cell type: PBMC, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: Tat, None) , ( cell type: primary peripheral blood mononuclear cells, None) , ( protein: Tat protein, None) , ( cell type: PBMC, None) 