5930
The requirement for TNF is further confirmed by the observation that the inability of the intermediate CD4 (+) CD8(-) CD3 (-) thymocytes to replicate the virus is associated with a defect in TNF production during their interaction with TEC and correlates with the absence of nuclear NF-kappaB activity in these freshly isolated thymocytes .
 ( protein: TNF, None) , ( protein: CD4, None) , ( cell type: TEC, None) , ( cell type: intermediate CD4 (+) CD8(-) CD3 (-) thymocytes, None) , ( protein: NF-kappaB, None) , ( protein: CD3, None) , ( cell type: freshly isolated thymocytes, None) , ( protein: TNF, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF, None) , ( protein: CD4, None) , ( cell type: TEC, None) , ( cell type: intermediate CD4 (+) CD8(-) CD3 (-) thymocytes, None) , ( protein: NF-kappaB, None) , ( protein: CD3, None) , ( cell type: freshly isolated thymocytes, None) , ( protein: TNF, None) 