378
Finally, we show by both footprint analysis and functional assays that the ability of the G + C-rich region to increase alpha-globin promoter activity from a stably integrated alpha-globin gene is mediated by its multiple binding sites for the transcription factor Sp1 .
 ( protein: transcription factor, None) , ( DNA: alpha-globin gene, None) , ( DNA: multiple binding sites, None) , ( DNA: alpha-globin promoter, None) , ( DNA: G + C-rich region, None) , ( protein: Sp1, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: transcription factor, None) , ( DNA: alpha-globin gene, None) , ( DNA: multiple binding sites, None) , ( DNA: alpha-globin promoter, None) , ( DNA: G + C-rich region, None) , ( protein: Sp1, None) 