6650
Among upstream signaling molecules of ERK , Shc was constitutively associated with Grb2 and was not tyrosine -phosphorylated by GM-CSF and FMLP , and Sos1 and c-Raf-1 were not phosphorylated by GM-CSF , IL-3, TNF, and FMLP in monocytes , whereas all these signaling molecules were affected and/or utilized by GM-CSF in MO7e cells .
 ( protein: Sos1, None) , ( protein: c-Raf-1, None) , ( protein: Shc, None) , ( protein: upstream signaling molecules, None) , ( protein: GM-CSF, None) , ( cell type: monocytes, None) , ( protein: GM-CSF, None) , ( protein: ERK, None) , ( cell type: MO7e cells, None) , ( protein: GM-CSF, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: Sos1, None) , ( protein: c-Raf-1, None) , ( protein: Shc, None) , ( protein: upstream signaling molecules, None) , ( protein: GM-CSF, None) , ( cell type: monocytes, None) , ( protein: GM-CSF, None) , ( protein: ERK, None) , ( cell type: MO7e cells, None) , ( protein: GM-CSF, None) 