2442
Production of IL-8 by hypoxic ECs occurred concomitantly with both increased levels of IL-8 mRNA , based on polymerase chain reaction analysis , and increased IL-8 transcription , based on nuclear run-on assays .
 ( RNA: IL-8 mRNA, None) , ( protein: IL-8, None) , ( cell type: ECs, None) , ( protein: IL-8, None) , ( protein: IL-8, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( RNA: IL-8 mRNA, None) , ( protein: IL-8, None) , ( cell type: ECs, None) , ( protein: IL-8, None) , ( protein: IL-8, None) 