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In HeLa cells stably transfected with the HIV-1 tat gene ( HeLa-tat cells ), expression of the Tat protein enhanced both TNF -induced activation of NF-kappa B and TNF -mediated cytotoxicity .
 ( protein: TNF, None) , ( cell line: HeLa-tat cells, None) , ( protein: Tat protein, None) , ( DNA: HIV-1 tat gene, None) , ( cell line: HeLa cells, None) , ( protein: TNF, None) , ( protein: NF-kappa B, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF, None) , ( cell line: HeLa-tat cells, None) , ( protein: Tat protein, None) , ( DNA: HIV-1 tat gene, None) , ( cell line: HeLa cells, None) , ( protein: TNF, None) , ( protein: NF-kappa B, None) 