1053
Transient chloramphenicol acetyltransferase assay revealed that the region containing E2F binding site had a strong promoter activity , and introduction of the mutation at the E2F binding site resulted in a significant loss of the activity.
 ( DNA: E2F binding site, None) , ( protein: E2F, None) , ( protein: E2F, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: E2F binding site, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: E2F binding site, None) , ( protein: E2F, None) , ( protein: E2F, None) , ( protein: chloramphenicol acetyltransferase, None) , ( DNA: E2F binding site, None) 