3646
Addition of the -176 to -96 sequence to the 4X NF-AT-Oct construct did not impart the ability to be affected by anergy, but addition of the -236 to -96 sequence did, demonstrating that anergy is an active inhibitory process and that more than the presence of the -150 AP-1 binding site ( -152 to -147 ) is required to mediate the effect.
 ( DNA: -150 AP-1 binding site, None) , ( DNA: 4X NF-AT-Oct construct, None) , ( DNA: -152 to -147, None) , ( DNA: 4X NF-AT-Oct, None) , ( DNA: -176 to -96 sequence, None) , ( DNA: -236 to -96 sequence, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: -150 AP-1 binding site, None) , ( DNA: 4X NF-AT-Oct construct, None) , ( DNA: -152 to -147, None) , ( DNA: 4X NF-AT-Oct, None) , ( DNA: -176 to -96 sequence, None) , ( DNA: -236 to -96 sequence, None) 