5772
After up to 90 min exposure time ELISA , EMSA ( electromobility shift assay ) and RT- PCR were used to measure protein tyrosine kinase activity , protein activity of NF-kappaB and cytokine ( IL-1beta , IL-6 , TNF-alpha ) specific mRNA levels in BEAS-2B cells .
 ( protein: cytokine, None) , ( RNA: mRNA, None) , ( protein: protein tyrosine kinase, None) , ( protein: TNF-alpha, None) , ( protein: IL-1beta, None) , ( protein: IL-6, None) , ( cell type: BEAS-2B cells, None) , ( protein: NF-kappaB, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: cytokine, None) , ( RNA: mRNA, None) , ( protein: protein tyrosine kinase, None) , ( protein: TNF-alpha, None) , ( protein: IL-1beta, None) , ( protein: IL-6, None) , ( cell type: BEAS-2B cells, None) , ( protein: NF-kappaB, None) 