12604
Binding to kappaB sequence was accompanied by an initial decrease and subsequent increase in the cytoplasmic IkappaBalpha levels , as detected by Western blotting using an anti- IkappaBalpha antibody .
 ( protein: IkappaBalpha, None) , ( protein: anti- IkappaBalpha antibody, None) , ( protein: IkappaBalpha, None) , ( DNA: kappaB sequence, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: IkappaBalpha, None) , ( protein: anti- IkappaBalpha antibody, None) , ( protein: IkappaBalpha, None) , ( DNA: kappaB sequence, None) 