2451
Proteolytic activity of the enzyme was required for this effect since diisopropyl fluorophosphate-thrombin failed to increase [ Ca2+ ]i .
 ( protein: diisopropyl fluorophosphate-thrombin, None) , ( protein: enzyme, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: diisopropyl fluorophosphate-thrombin, None) , ( protein: enzyme, None) 