14220
Additionally, Nef expressing cells were transiently transfected with a plasmid in which HIV-1 AP-1 DNA recognition sequences were cloned downstream of the chloramphenicol acetyltransferase ( CAT ) gene .
 ( DNA: HIV-1 AP-1 DNA recognition sequences, None) , ( protein: Nef, None) , ( DNA: chloramphenicol acetyltransferase ( CAT ) gene, None) , ( cell line: Nef expressing cells, None) , ( protein: CAT, None) , ( protein: chloramphenicol acetyltransferase, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: HIV-1 AP-1 DNA recognition sequences, None) , ( protein: Nef, None) , ( DNA: chloramphenicol acetyltransferase ( CAT ) gene, None) , ( cell line: Nef expressing cells, None) , ( protein: CAT, None) , ( protein: chloramphenicol acetyltransferase, None) 