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Using a combination of bacterial motility assays in platelet extracts with Escherichia coli expressing the Shigella IcsA protein and in vitro analysis of reconstituted systems from purified proteins , we show here that the bacterial protein IcsA binds N-WASP and activates it in a Cdc42 -like fashion .
 ( protein: purified proteins, None) , ( protein: bacterial protein IcsA, None) , ( protein: N-WASP, None) , ( protein: Cdc42, None) , ( protein: Shigella IcsA protein, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: purified proteins, None) , ( protein: bacterial protein IcsA, None) , ( protein: N-WASP, None) , ( protein: Cdc42, None) , ( protein: Shigella IcsA protein, None) 