11716
The capacity of Syk to reconstitute the MAPK response required the catalytic activity of Syk , an intact autophosphorylation site ( Y518 and Y519 ), both Src homology 2 domains and it was blocked by the inhibitory N17-mutated dominant-negative Ras construct .
 ( protein: Src homology 2 domains, None) , ( protein: Syk, None) , ( protein: Syk, None) , ( protein: inhibitory N17-mutated dominant-negative Ras construct, None) , ( protein: autophosphorylation site, None) , ( protein: MAPK, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: Src homology 2 domains, None) , ( protein: Syk, None) , ( protein: Syk, None) , ( protein: inhibitory N17-mutated dominant-negative Ras construct, None) , ( protein: autophosphorylation site, None) , ( protein: MAPK, None) 