10669
To further explore the involvement of PI3-K in T cell activation , we created a set of potentially dominant negative PI3-K constructs comprising individual or tandem domains of the regulatory p85 subunit and tested their effect on downstream signaling events like Erk2 activation and transcription from an NFAT ( nuclear factor of activated T cells ) element taken from the interleukin-2 promoter .
 ( DNA: NFAT ( nuclear factor of activated T cells ) element, None) , ( protein: NFAT, None) , ( protein: regulatory p85 subunit, None) , ( DNA: interleukin-2 promoter, None) , ( DNA: tandem domains, None) , ( protein: PI3-K, None) , ( protein: nuclear factor of activated T cells, None) , ( protein: Erk2, None) , ( DNA: dominant negative PI3-K constructs, None) , ( cell type: T cells, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: NFAT ( nuclear factor of activated T cells ) element, None) , ( protein: NFAT, None) , ( protein: regulatory p85 subunit, None) , ( DNA: interleukin-2 promoter, None) , ( DNA: tandem domains, None) , ( protein: PI3-K, None) , ( protein: nuclear factor of activated T cells, None) , ( protein: Erk2, None) , ( DNA: dominant negative PI3-K constructs, None) , ( cell type: T cells, None) 