6489
First, STAT4 was required for the IL-12 -dependent transactivation of an IRF-1 reporter construct , and second, STAT4 binding to the IRF-1 promoter was shown using EMSA .
 ( DNA: IRF-1 promoter, None) , ( DNA: IRF-1 reporter construct, None) , ( protein: IL-12, None) , ( protein: IRF-1, None) , ( protein: STAT4, None) , ( protein: STAT4, None) , ( protein: IRF-1, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: IRF-1 promoter, None) , ( DNA: IRF-1 reporter construct, None) , ( protein: IL-12, None) , ( protein: IRF-1, None) , ( protein: STAT4, None) , ( protein: STAT4, None) , ( protein: IRF-1, None) 