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Here, we show that zinc addition in a serum- and lipopolysaccharide-free cell culture system leads to significantly enhanced levels of interleukin 1 beta ( IL-1 beta ) and tumour necrosis factor alpha ( TNF-alpha ) and to expression of the corresponding mRNA in human peripheral blood mononuclear cells ( PBMC ).
 ( RNA: mRNA, None) , ( cell type: human peripheral blood mononuclear cells, None) , ( protein: TNF-alpha, None) , ( protein: tumour necrosis factor alpha, None) , ( cell type: PBMC, None) , ( protein: IL-1 beta, None) , ( protein: interleukin 1 beta, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( RNA: mRNA, None) , ( cell type: human peripheral blood mononuclear cells, None) , ( protein: TNF-alpha, None) , ( protein: tumour necrosis factor alpha, None) , ( cell type: PBMC, None) , ( protein: IL-1 beta, None) , ( protein: interleukin 1 beta, None) 