4190
In contrast to freshly isolated T cells , in vitro-cultured T cells did not require costimulation for cytokine secretion in response to anti-CD3 alone.
 ( cell line: vitro-cultured T cells, None) , ( cell type: freshly isolated T cells, None) , ( protein: cytokine, None) , ( protein: anti-CD3, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell line: vitro-cultured T cells, None) , ( cell type: freshly isolated T cells, None) , ( protein: cytokine, None) , ( protein: anti-CD3, None) 