4650
By using mutated promoter- PLAP plasmids , we established that these NF-kappa B sites were necessary for induction of TNF-alpha transcription on stimulation with LPS .
 ( protein: TNF-alpha, None) , ( protein: PLAP, None) , ( DNA: NF-kappa B sites, None) , ( protein: NF-kappa B, None) , ( DNA: mutated promoter- PLAP plasmids, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF-alpha, None) , ( protein: PLAP, None) , ( DNA: NF-kappa B sites, None) , ( protein: NF-kappa B, None) , ( DNA: mutated promoter- PLAP plasmids, None) 