11911
We demonstrate that: 1) RANTES promoter activity is up-regulated by PMA plus ionomycin , coexpression of the p65 subunit of nuclear factor (NF)-kappa B , the proinflammatory cytokines TNF-alpha and IL-1 beta , and the CD28 costimulatory pathway ; 2) the RANTES promoter region contains four NF-kappa B binding sites at positions -30 , -44 , -213 , and -579 relative to the transcription start site ; 3) one site (-213) is an NF-AT ( nuclear factor of activated T cells ) binding site that also has weak affinity to NF-kappa B , and the most distal site (-579) also serves as a CD28-responsive element ; and 4) mutation on any of those NF-kappa B sites or coexpression of I kappa B alpha ( cytoplasmic inhibitor of NF-kappa B ) markedly reduced the promoter activity .
 ( DNA: transcription start site, None) , ( protein: RANTES, None) , ( protein: RANTES, None) , ( protein: proinflammatory cytokines, None) , ( protein: NF-AT, None) , ( DNA: NF-kappa B binding sites, None) , ( protein: I kappa B alpha, None) , ( protein: IL-1 beta, None) , ( protein: NF-kappa B, None) , ( protein: NF-kappa B, None) , ( DNA: RANTES promoter region, None) , ( protein: NF-kappa B, None) , ( protein: cytoplasmic inhibitor of NF-kappa B, None) , ( DNA: CD28-responsive element, None) , ( DNA: binding sites, None) , ( DNA: NF-AT ( nuclear factor of activated T cells ) binding site, None) , ( protein: p65 subunit, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor (NF)-kappa B, None) , ( protein: CD28, None) , ( protein: nuclear factor of activated T cells, None) , ( protein: TNF-alpha, None) , ( DNA: NF-kappa B sites, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: transcription start site, None) , ( protein: RANTES, None) , ( protein: RANTES, None) , ( protein: proinflammatory cytokines, None) , ( protein: NF-AT, None) , ( DNA: NF-kappa B binding sites, None) , ( protein: I kappa B alpha, None) , ( protein: IL-1 beta, None) , ( protein: NF-kappa B, None) , ( protein: NF-kappa B, None) , ( DNA: RANTES promoter region, None) , ( protein: NF-kappa B, None) , ( protein: cytoplasmic inhibitor of NF-kappa B, None) , ( DNA: CD28-responsive element, None) , ( DNA: binding sites, None) , ( DNA: NF-AT ( nuclear factor of activated T cells ) binding site, None) , ( protein: p65 subunit, None) , ( protein: NF-kappa B, None) , ( protein: nuclear factor (NF)-kappa B, None) , ( protein: CD28, None) , ( protein: nuclear factor of activated T cells, None) , ( protein: TNF-alpha, None) , ( DNA: NF-kappa B sites, None) 