7404
Our previous studies pertaining to macrophage response to LPS identified a novel DNA-binding domain located from -550 to -487 in the human TNF-alpha promoter that contains transcriptional activity , but lacks any known NF-kappaB -binding sites .
 ( protein: TNF-alpha, None) , ( DNA: -binding sites, None) , ( protein: NF-kappaB, None) , ( DNA: human TNF-alpha promoter, None) , ( protein: DNA-binding domain, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: TNF-alpha, None) , ( DNA: -binding sites, None) , ( protein: NF-kappaB, None) , ( DNA: human TNF-alpha promoter, None) , ( protein: DNA-binding domain, None) 