2899
TCR signaling was triggered by treating Jurkat T cells with PHA or anti-CD3 Abs , and NF-kappaB activation was monitored by electrophoretic mobility shift assays and/or by kappaB -dependent reporter assays .
 ( protein: kappaB, None) , ( protein: PHA, None) , ( protein: NF-kappaB, None) , ( protein: anti-CD3 Abs, None) , ( protein: TCR, None) , ( cell line: Jurkat T cells, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: kappaB, None) , ( protein: PHA, None) , ( protein: NF-kappaB, None) , ( protein: anti-CD3 Abs, None) , ( protein: TCR, None) , ( cell line: Jurkat T cells, None) 