12209
When mutations that abolished factor binding in bandshift assays were introduced into a Wp reporter construct , the loss of any one of the three UAS1 binding sites was sufficient to reduce promoter activity by 10- to 30-fold in B cells .
 ( cell type: B cells, None) , ( DNA: binding sites, None) , ( DNA: UAS1, None) , ( DNA: UAS1 binding sites, None) , ( DNA: Wp reporter construct, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( cell type: B cells, None) , ( DNA: binding sites, None) , ( DNA: UAS1, None) , ( DNA: UAS1 binding sites, None) , ( DNA: Wp reporter construct, None) 