3820
Transfection of trans-dominant negative expression vectors of ras and raf , together with AP-1 -dependent reporter constructs , as well as Western blot analysis using anti-ERK ( extracellular signal-regulated kinase ) antibodies , indicated that the Ras/Raf/ERK pathway did not appear to mediate the effect of the antioxidant.
 ( protein: extracellular signal-regulated kinase, None) , ( DNA: ras, None) , ( DNA: AP-1 -dependent reporter constructs, None) , ( DNA: trans-dominant negative expression vectors, None) , ( protein: anti-ERK ( extracellular signal-regulated kinase ) antibodies, None) , ( DNA: raf, None) , ( protein: AP-1, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( protein: extracellular signal-regulated kinase, None) , ( DNA: ras, None) , ( DNA: AP-1 -dependent reporter constructs, None) , ( DNA: trans-dominant negative expression vectors, None) , ( protein: anti-ERK ( extracellular signal-regulated kinase ) antibodies, None) , ( DNA: raf, None) , ( protein: AP-1, None) 