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To understand the mechanism by which PRL regulates the biphasic expression of IRF-1 , we cloned the rat IRF-1 gene and functionally characterized the IRF-1 promoter .
 ( DNA: IRF-1 promoter, None) , ( protein: IRF-1, None) , ( DNA: rat IRF-1 gene, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) 
Locate the role in the text that participated in the event based on the event type and return it in the event list. 
 Option: Event type: RNA,cell type,protein,cell line,DNA, Arguments type: . 
Text: {0} 
Answer:
 ( DNA: IRF-1 promoter, None) , ( protein: IRF-1, None) , ( DNA: rat IRF-1 gene, None) , ( protein: IRF-1, None) , ( protein: IRF-1, None) , ( protein: PRL, None) 