Scientists are advancing their research with Double Helix Light Engineering.
Joshua Yoon, Colin J. Comerci, Lucien E. Weiss, Ljiljana Milenkovic, Tim Stearns, and W. E. Moerner, Biophys. J. 116, 319-329 (2019) (DOI: 10.1016/j.bpj.2018.11.3136, published online 7 December 2018).
Rehman, S. A., Carr, A. R., Lenz, M. O., Lee, S. F., & O'Holleran, K.
Optics Express, 26(4), 4631. (2018).
Gustavsson, A.-K., Petrov, P. N., Lee, M. Y., Shechtman, Y., & Moerner, W. E.
Communications 2018 9:1, 9(1), 123. (2018).
Three-Dimensional Tracking of Interfacial Hopping Diffusion.
Wang, D., Wu, H., & Schwartz, D. K.
Enhanced information content for three-dimensional localization and tracking using the double-helix point spread function with variable-angle illumination epifluorescence microscopy.
Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function.
ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure.
Real-time adaptive drift correction for super-resolution localization microscopy.
Grover, G., Mohrman, W. & Piestun, R.
Opt. Express 23, 23887–98 (2015).
Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging.
Nat. Rev. Microbiol. 12, 9–22 (2014).
Three-dimensional super-resolution and localization of dense clusters of single molecules.
Barsic, A., Grover, G. & Piestun, R.
Sci. Rep. 4, 5388 (2014).
The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.
Backlund, M. P., Lew, M. D., Backer, A. S., Sahl, S. J. & Moerner, W. E.
Extending single-molecule microscopy using optical Fourier processing.
Backer, A. S. & Moerner, W. E.
J. Phys. Chem. B 118, 8313–29 (2014).
Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy.
Lee, M. K., Rai, P., Williams, J., Twieg, R. J. & Moerner, W. E.
J. Am. Chem. Soc. 136, 14003–6 (2014).
Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope.
Backlund, M. P., Joyner, R., Weis, K. & Moerner, W. E.
Mol. Biol. Cell 25, 3619–29 (2014).
Proc. Natl. Acad. Sci. U. S. A. 111, E2046–55 (2014).
The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation.
Backlund, M. P. et al.
SPIE–the Int. Soc. Opt. Eng. 8590, 85900 (2013).
Super-resolution fluorescence imaging with single molecules.
Sahl, S. J. & Moerner, W. E.
Curr. Opin. Struct. Biol. 23, 778–87 (2013).
Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.
Nano Lett. 13, 987–93 (2013).
Depth estimation and image recovery using broadband, incoherent illumination with engineered point spread functions [Invited].
Quirin, S. & Piestun, R.
Appl. Opt. 52, A367–76 (2013).
Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration.
Ghosh, S. & Preza, C.
J. Biomed. Opt. 18, 036010 (2013).
The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision.
Lee, H.-L. D., Sahl, S. J., Lew, M. D. & Moerner, W. E.
Appl. Phys. Lett. 100, 153701–1537013 (2012).
Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE).
Grover, G., DeLuca, K., Quirin, S., DeLuca, J. & Piestun, R.
Opt. Express 20, 26681–95 (2012).
Limits of 3D dipole localization and orientation estimation for single-molecule imaging: towards Green's tensor engineering.
Proc. Natl. Acad. Sci. U. S. A. 109, 19087–92 (2012).
Opt. Express 19, 3835–42 (2011).
Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging.
Grover, G., Quirin, S., Fiedler, C. & Piestun, R.
Biomed. Opt. Express 2, 3010–20 (2011).
Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function.
Thompson, M. A., Casolari, J. M., Badieirostami, M., Brown, P. O. & Moerner, W. E.
Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function.
Thompson, M. A., Lew, M. D., Badieirostami, M. & Moerner, W. E.
Nano Lett. 10, 211–8 (2010).
Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane.
Badieirostami, M., Lew, M. D., Thompson, M. A. & Moerner, W. E.
Appl. Phys. Lett. 97, 161103 (2010).
Performance limits on three-dimensional particle localization in photon-limited microscopy.
Grover, G., Pavani, S. R. P. & Piestun, R.
Opt. Lett. 35, 3306–8 (2010).
In vivo Three-Dimensional Superresolution Fluorescence Tracking using a Double-Helix Point Spread Function.
Lew, M. D., Thompson, M. A., Badieirostami, M. & Moerner, W. E.
Proc. SPIE–the Int. Soc. Opt. Eng. 7571, 75710Z (2010).
Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system.
Pavani, S. R. P., Greengard, A. & Piestun, R.
Appl. Phys. Lett. 95, 021103 (2009).
Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.
Pavani, S. R. P. et al.
Proc. Natl. Acad. Sci. U. S. A. 106, 2995–9 (2009).
Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system.
Pavani, S. R. P. & Piestun, R.
Opt. Express 16, 22048 (2008).
High-efficiency rotating point spread functions.
Opt. Express 16, 3484 (2008).
Greengard, A., Schechner, Y. Y. & Piestun, R.
Opt. Lett. 31, 181 (2006).