M65 Apoptosis + Necrosis. Human intermediate filament protein keratin 18 (K18)*. Released from human epithelial cells.
supernatants from cells or tissues.
Store the samples at 2–8 °C up to 4 hours. For longer periods, store samples at -20 °C or lower.
Avoid repeated freezing and thawing of specimens.
No high dose "hook effect" occur until 200000 U/l which is well above concentrations of M65-reactive material observed in cell culture or serum/plasma samples.
Quantitative measurement of total soluble keratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.
The M65 EpiDeath® ELISA can be combined with the M30 Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients.