Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Learn more about our entire cell analysis portfolio and identify the best solution for your research needs.
Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
Controller Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability.
Software Design and analyze assay templates on Controller or Wave desktop.
Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
Find the perfect instrument for your analysis needs with this hepful guide.
Glycolysis is a critical process in both normal cellular function and diseases such as cancer. Cells readily switch between this energy producing pathway and mitochondrial respiration, depending on energy demand and nutrient availability. As such, knowing how much glycolysis is being used by cells at any moment in time is important for understanding the interaction between metabolic activity and cell function. When glycolysis proceeds to completion, lactate and protons are extruded from the cell. The Seahorse XF Glycolytic Rate Assay Kit measures the resulting acidification rates and accounts for mitochondrial acidification to deliver the most accurate acidification-based measurement available.
Seahorse technology is based on measuring the rate of extracellular fluxes of analytes in the media surrounding cells. Seahorse assays enable quantification of cellular bioenergetics by measuring the two energy yielding pathways, mitochondrial respiration and glycolysis, in a sensitive and convenient microplate format. A cell's ability to respond to increased energy, biosynthetic and redox demands are in a large part influenced by two fundamental pathways, mitochondrial respiration and glycolysis.
XF Extracellular Flux technology is commonly used to measure cellular bioenergetics, and determine the role of metabolism in regulating cell phenotype. From measuring basic intracellular mitochondrial function, XF analysis has now evolved to employing multiple assays for the measurement of mitochondrial substrate selectivity and glycolysis. In addition XF Analyzers are also used to measure the function of isolated mitochondria, enabling the examination of mitochondria from multiple samples simultaneously, saving valuable time and resources.
Cell Reference Database - search scientific publications that reference/cite Agilent Cell Analysis products and technology by cell type, cell line, research area, assay, or author.
Publications Database - publications, in which Agilent Cell Analysis data is included, linked to PubMed.gov.
XF Instrument Selection Guide - general specifications and features of the different Seahorse Analyzer models.
Basic Procedures & Assay Protocols - guides to help users through the steps required to perform assays.
Cell Analysis Travel Awards - travel awards for researchers who present a poster or presentation citing data generated using Agilent Cell Analysis technology.
XF Analyzer Grant Expeditor Package - simplify your grant application process with the Agilent Seahorse XF Analyzer Grant Expeditor Package.
XF Core Facilities - facilities that provide access to Agilent Seahorse XF technology, including advice in experimental design, data analysis, basic training, and bioinformatics on a cost recovery basis.
Lab Instrument Service Agreements – instrument service plans that maintain instrument performance and keep laboratory operations running smoothly and consistently.