Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
Jonathan A. Dranoff, Anatoly I. Masyuk, Emma A. Kruglov, Nicholas F. LaRusso, Michael H. Nathanson
Extracellular nucleotides may be important regulators of bile ductular secretion, because cholangiocytes express P2Y ATP receptors and nucleotides are found in bile. However, the expression, distribution, and function of specific P2Y receptor subtypes in cholangiocytes are unknown. Thus our aim was to determine the subtypes, distribution, and role in secretion of P2Y receptors expressed by cholangiocytes. The molecular subtypes of P2Y receptors were determined by RT-PCR. Functional studies measuring cytosolic Ca2+ (Cai2+) signals and bile ductular pH were performed in isolated, microperfused intrahepatic bile duct units (IBDUs). PCR products corresponding to P2Y1, P2Y2, P2Y4, P2Y6, and P2X4 receptor subtypes were identified. Luminal perfusion of ATP into IBDUs induced increases in Ca2+ that were inhibited by apyrase and suramin. Luminal ATP, ADP, 2-methylthio-adenosine 5′-triphosphate, UTP, and UDP each increased Cai2+. Basolateral addition of adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S), but not ATP, to the perifusing bath increased Cai2+. IBDU perfusion with ATP-γ-S induced net bile ductular alkalization. Cholangiocytes express multiple P2Y receptor subtypes that are expressed at the apical plasma membrane domain. P2Y receptors are also expressed on the basolateral domain, but their activation is attenuated by nucleotide hydrolysis. Activation of ductular P2Y receptors induces net ductular alkalization, suggesting that nucleotide signaling may be an important regulator of bile secretion by the liver.
G1059-G1067
American Journal of Physiology - Gastrointestinal and Liver Physiology
4 44-4
https://doi.org/10.1152/ajpgi.2001.281.4.g1059
Nucleoside triphosphate diphosphohydrolase
Purinoceptor
Secretion
10.1152/ajpgi.2001.281.4.g1059
Dive into the research topics of 'Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia'. Together they form a unique fingerprint.
Purinergic P2 Receptors Medicine & Life Sciences 100%
Bile Ducts Medicine & Life Sciences 74%
Bile Medicine & Life Sciences 72%
Intrahepatic Bile Ducts Medicine & Life Sciences 58%
Epithelium Medicine & Life Sciences 55%
Nucleotides Medicine & Life Sciences 47%
purinoceptor P2Y4 Medicine & Life Sciences 25%
Dranoff, J. A., Masyuk, A. I., Kruglov, E. A., LaRusso, N. F., & Nathanson, M. H. (2001). Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. American Journal of Physiology - Gastrointestinal and Liver Physiology, 281(4 44-4), G1059-G1067. https://doi.org/10.1152/ajpgi.2001.281.4.g1059
Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. / Dranoff, Jonathan A.; Masyuk, Anatoly I.; Kruglov, Emma A. et al.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 281, No. 4 44-4, 2001, p. G1059-G1067.
Dranoff, JA, Masyuk, AI, Kruglov, EA, LaRusso, NF & Nathanson, MH 2001, 'Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 281, no. 4 44-4, pp. G1059-G1067. https://doi.org/10.1152/ajpgi.2001.281.4.g1059
Dranoff JA, Masyuk AI, Kruglov EA, LaRusso NF, Nathanson MH. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2001;281(4 44-4):G1059-G1067. doi: 10.1152/ajpgi.2001.281.4.g1059
Dranoff, Jonathan A. ; Masyuk, Anatoly I. ; Kruglov, Emma A. et al. / Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2001 ; Vol. 281, No. 4 44-4. pp. G1059-G1067.
@article{33fce1c14f294d31a5abe161b9fb8c4a,
title = "Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia",
abstract = "Extracellular nucleotides may be important regulators of bile ductular secretion, because cholangiocytes express P2Y ATP receptors and nucleotides are found in bile. However, the expression, distribution, and function of specific P2Y receptor subtypes in cholangiocytes are unknown. Thus our aim was to determine the subtypes, distribution, and role in secretion of P2Y receptors expressed by cholangiocytes. The molecular subtypes of P2Y receptors were determined by RT-PCR. Functional studies measuring cytosolic Ca2+ (Cai2+) signals and bile ductular pH were performed in isolated, microperfused intrahepatic bile duct units (IBDUs). PCR products corresponding to P2Y1, P2Y2, P2Y4, P2Y6, and P2X4 receptor subtypes were identified. Luminal perfusion of ATP into IBDUs induced increases in Ca2+ that were inhibited by apyrase and suramin. Luminal ATP, ADP, 2-methylthio-adenosine 5′-triphosphate, UTP, and UDP each increased Cai2+. Basolateral addition of adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S), but not ATP, to the perifusing bath increased Cai2+. IBDU perfusion with ATP-γ-S induced net bile ductular alkalization. Cholangiocytes express multiple P2Y receptor subtypes that are expressed at the apical plasma membrane domain. P2Y receptors are also expressed on the basolateral domain, but their activation is attenuated by nucleotide hydrolysis. Activation of ductular P2Y receptors induces net ductular alkalization, suggesting that nucleotide signaling may be an important regulator of bile secretion by the liver.",
keywords = "Calcium, Chloride, Nucleoside triphosphate diphosphohydrolase, Nucleotide, Purinoceptor, Secretion",
author = "Dranoff, {Jonathan A.} and Masyuk, {Anatoly I.} and Kruglov, {Emma A.} and LaRusso, {Nicholas F.} and Nathanson, {Michael H.}",
doi = "10.1152/ajpgi.2001.281.4.g1059",
pages = "G1059--G1067",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
publisher = "American Physiological Society",
number = "4 44-4",
T1 - Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
AU - Dranoff, Jonathan A.
AU - Masyuk, Anatoly I.
AU - Kruglov, Emma A.
AU - LaRusso, Nicholas F.
AU - Nathanson, Michael H.
N2 - Extracellular nucleotides may be important regulators of bile ductular secretion, because cholangiocytes express P2Y ATP receptors and nucleotides are found in bile. However, the expression, distribution, and function of specific P2Y receptor subtypes in cholangiocytes are unknown. Thus our aim was to determine the subtypes, distribution, and role in secretion of P2Y receptors expressed by cholangiocytes. The molecular subtypes of P2Y receptors were determined by RT-PCR. Functional studies measuring cytosolic Ca2+ (Cai2+) signals and bile ductular pH were performed in isolated, microperfused intrahepatic bile duct units (IBDUs). PCR products corresponding to P2Y1, P2Y2, P2Y4, P2Y6, and P2X4 receptor subtypes were identified. Luminal perfusion of ATP into IBDUs induced increases in Ca2+ that were inhibited by apyrase and suramin. Luminal ATP, ADP, 2-methylthio-adenosine 5′-triphosphate, UTP, and UDP each increased Cai2+. Basolateral addition of adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S), but not ATP, to the perifusing bath increased Cai2+. IBDU perfusion with ATP-γ-S induced net bile ductular alkalization. Cholangiocytes express multiple P2Y receptor subtypes that are expressed at the apical plasma membrane domain. P2Y receptors are also expressed on the basolateral domain, but their activation is attenuated by nucleotide hydrolysis. Activation of ductular P2Y receptors induces net ductular alkalization, suggesting that nucleotide signaling may be an important regulator of bile secretion by the liver.
AB - Extracellular nucleotides may be important regulators of bile ductular secretion, because cholangiocytes express P2Y ATP receptors and nucleotides are found in bile. However, the expression, distribution, and function of specific P2Y receptor subtypes in cholangiocytes are unknown. Thus our aim was to determine the subtypes, distribution, and role in secretion of P2Y receptors expressed by cholangiocytes. The molecular subtypes of P2Y receptors were determined by RT-PCR. Functional studies measuring cytosolic Ca2+ (Cai2+) signals and bile ductular pH were performed in isolated, microperfused intrahepatic bile duct units (IBDUs). PCR products corresponding to P2Y1, P2Y2, P2Y4, P2Y6, and P2X4 receptor subtypes were identified. Luminal perfusion of ATP into IBDUs induced increases in Ca2+ that were inhibited by apyrase and suramin. Luminal ATP, ADP, 2-methylthio-adenosine 5′-triphosphate, UTP, and UDP each increased Cai2+. Basolateral addition of adenosine 5′-O-(3-thiotriphosphate) (ATP-γ-S), but not ATP, to the perifusing bath increased Cai2+. IBDU perfusion with ATP-γ-S induced net bile ductular alkalization. Cholangiocytes express multiple P2Y receptor subtypes that are expressed at the apical plasma membrane domain. P2Y receptors are also expressed on the basolateral domain, but their activation is attenuated by nucleotide hydrolysis. Activation of ductular P2Y receptors induces net ductular alkalization, suggesting that nucleotide signaling may be an important regulator of bile secretion by the liver.
KW - Chloride
KW - Nucleoside triphosphate diphosphohydrolase
KW - Nucleotide
KW - Purinoceptor
KW - Secretion
U2 - 10.1152/ajpgi.2001.281.4.g1059
DO - 10.1152/ajpgi.2001.281.4.g1059
SP - G1059-G1067
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 4 44-4