Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves
Jennifer D. Tedman-Jones, Rita Lei, Florence Jay, Georgina Fabro, Xuemei Li, Wolf‐Dieter Reiter, Charles Brearley, Jonathan D. G. Jones
A screen was established for mutants in which the plant defence response is de-repressed. The pathogen-inducible isochorismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic line generated in which the ICS1:luc fusion is co-regulated with ICS1. This line was mutagenized and M2 seedlings screened for constitutive ICS1:luc expression (cie). The cie mutants fall into distinct phenotypic classes based on tissue-specific localization of luciferase activity. One mutant, cie1, that shows constitutive luciferase activity specifically in petioles, was chosen for further analysis. In addition to ICS1, PR and other defence-related genes are constitutively expressed in cie1 plants. The cie1 mutant is also characterized by an increased production of conjugated salicylic acid and reactive oxygen intermediates, as well as spontaneous lesion formation, all confined to petiole tissue. Significantly, defences activated in cie1 are sufficient to prevent infection by a virulent isolate of Hyaloperonospora parasitica, and this enhanced resistance response protects petiole tissue alone. Furthermore, cie1-mediated resistance, along with PR gene expression, is abolished in a sid2-1 mutant background, consistent with a requirement for salicylic acid. A positional cloning approach was used to identify cie1, which carries two point mutations in a gene required for cell wall biosynthesis and actin organization, MUR3. A mur3 knockout mutant also resists infection by H. parasitica in its petioles and this phenotype is complemented by transformation with wild-type MUR3. We propose that perturbed cell wall biosynthesis may activate plant defence and provide a rationale for the cie1 and the mur3 knockout phenotypes.
The Plant Journal
https://doi.org/10.1111/j.1365-313X.2008.03636.x
10.1111/j.1365-313X.2008.03636.x
Tedman-Jones, J. D., Lei, R., Jay, F., Fabro, G., Li, X., Reiter, WD., Brearley, C., & Jones, J. D. G. (2008). Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves. The Plant Journal, 56(5), 691-703. https://doi.org/10.1111/j.1365-313X.2008.03636.x
Tedman-Jones, Jennifer D. ; Lei, Rita ; Jay, Florence ; Fabro, Georgina ; Li, Xuemei ; Reiter, Wolf‐Dieter ; Brearley, Charles ; Jones, Jonathan D. G. / Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves. In: The Plant Journal. 2008 ; Vol. 56, No. 5. pp. 691-703.
@article{1b991c37baa547fcb684c018be3f795a,
title = "Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves",
abstract = "A screen was established for mutants in which the plant defence response is de-repressed. The pathogen-inducible isochorismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic line generated in which the ICS1:luc fusion is co-regulated with ICS1. This line was mutagenized and M2 seedlings screened for constitutive ICS1:luc expression (cie). The cie mutants fall into distinct phenotypic classes based on tissue-specific localization of luciferase activity. One mutant, cie1, that shows constitutive luciferase activity specifically in petioles, was chosen for further analysis. In addition to ICS1, PR and other defence-related genes are constitutively expressed in cie1 plants. The cie1 mutant is also characterized by an increased production of conjugated salicylic acid and reactive oxygen intermediates, as well as spontaneous lesion formation, all confined to petiole tissue. Significantly, defences activated in cie1 are sufficient to prevent infection by a virulent isolate of Hyaloperonospora parasitica, and this enhanced resistance response protects petiole tissue alone. Furthermore, cie1-mediated resistance, along with PR gene expression, is abolished in a sid2-1 mutant background, consistent with a requirement for salicylic acid. A positional cloning approach was used to identify cie1, which carries two point mutations in a gene required for cell wall biosynthesis and actin organization, MUR3. A mur3 knockout mutant also resists infection by H. parasitica in its petioles and this phenotype is complemented by transformation with wild-type MUR3. We propose that perturbed cell wall biosynthesis may activate plant defence and provide a rationale for the cie1 and the mur3 knockout phenotypes.",
author = "Tedman-Jones, {Jennifer D.} and Rita Lei and Florence Jay and Georgina Fabro and Xuemei Li and Wolf‐Dieter Reiter and Charles Brearley and Jones, {Jonathan D. G.}",
doi = "10.1111/j.1365-313X.2008.03636.x",
journal = "The Plant Journal",
Tedman-Jones, JD, Lei, R, Jay, F, Fabro, G, Li, X, Reiter, WD, Brearley, C & Jones, JDG 2008, 'Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves', The Plant Journal, vol. 56, no. 5, pp. 691-703. https://doi.org/10.1111/j.1365-313X.2008.03636.x
Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves. / Tedman-Jones, Jennifer D.; Lei, Rita; Jay, Florence; Fabro, Georgina; Li, Xuemei; Reiter, Wolf‐Dieter; Brearley, Charles; Jones, Jonathan D. G.
In: The Plant Journal, Vol. 56, No. 5, 12.2008, p. 691-703.
T1 - Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves
AU - Tedman-Jones, Jennifer D.
AU - Lei, Rita
AU - Jay, Florence
AU - Fabro, Georgina
AU - Li, Xuemei
AU - Reiter, Wolf‐Dieter
AU - Brearley, Charles
AU - Jones, Jonathan D. G.
N2 - A screen was established for mutants in which the plant defence response is de-repressed. The pathogen-inducible isochorismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic line generated in which the ICS1:luc fusion is co-regulated with ICS1. This line was mutagenized and M2 seedlings screened for constitutive ICS1:luc expression (cie). The cie mutants fall into distinct phenotypic classes based on tissue-specific localization of luciferase activity. One mutant, cie1, that shows constitutive luciferase activity specifically in petioles, was chosen for further analysis. In addition to ICS1, PR and other defence-related genes are constitutively expressed in cie1 plants. The cie1 mutant is also characterized by an increased production of conjugated salicylic acid and reactive oxygen intermediates, as well as spontaneous lesion formation, all confined to petiole tissue. Significantly, defences activated in cie1 are sufficient to prevent infection by a virulent isolate of Hyaloperonospora parasitica, and this enhanced resistance response protects petiole tissue alone. Furthermore, cie1-mediated resistance, along with PR gene expression, is abolished in a sid2-1 mutant background, consistent with a requirement for salicylic acid. A positional cloning approach was used to identify cie1, which carries two point mutations in a gene required for cell wall biosynthesis and actin organization, MUR3. A mur3 knockout mutant also resists infection by H. parasitica in its petioles and this phenotype is complemented by transformation with wild-type MUR3. We propose that perturbed cell wall biosynthesis may activate plant defence and provide a rationale for the cie1 and the mur3 knockout phenotypes.
AB - A screen was established for mutants in which the plant defence response is de-repressed. The pathogen-inducible isochorismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic line generated in which the ICS1:luc fusion is co-regulated with ICS1. This line was mutagenized and M2 seedlings screened for constitutive ICS1:luc expression (cie). The cie mutants fall into distinct phenotypic classes based on tissue-specific localization of luciferase activity. One mutant, cie1, that shows constitutive luciferase activity specifically in petioles, was chosen for further analysis. In addition to ICS1, PR and other defence-related genes are constitutively expressed in cie1 plants. The cie1 mutant is also characterized by an increased production of conjugated salicylic acid and reactive oxygen intermediates, as well as spontaneous lesion formation, all confined to petiole tissue. Significantly, defences activated in cie1 are sufficient to prevent infection by a virulent isolate of Hyaloperonospora parasitica, and this enhanced resistance response protects petiole tissue alone. Furthermore, cie1-mediated resistance, along with PR gene expression, is abolished in a sid2-1 mutant background, consistent with a requirement for salicylic acid. A positional cloning approach was used to identify cie1, which carries two point mutations in a gene required for cell wall biosynthesis and actin organization, MUR3. A mur3 knockout mutant also resists infection by H. parasitica in its petioles and this phenotype is complemented by transformation with wild-type MUR3. We propose that perturbed cell wall biosynthesis may activate plant defence and provide a rationale for the cie1 and the mur3 knockout phenotypes.
U2 - 10.1111/j.1365-313X.2008.03636.x
DO - 10.1111/j.1365-313X.2008.03636.x
JO - The Plant Journal
JF - The Plant Journal
Tedman-Jones JD, Lei R, Jay F, Fabro G, Li X, Reiter WD et al. Characterization of Arabidopsis mur3 mutations that result in constitutive activation of defence in petioles, but not leaves. The Plant Journal. 2008 Dec;56(5):691-703. https://doi.org/10.1111/j.1365-313X.2008.03636.x