Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white
Chiu Lan Hsieh, Chiung Chi Peng, Li Yun Lin, Yu Ting Cheng, Kuan Chou Chen, Robert Y. Peng
Graduate Institute of Clinical Medicine
The chorioallantoic membrane (CAM) assays have been intensively used to determine angiogenesis and anti-angiogenesis of medicines. In view of bioactivity, this technique should be performed with kinetic control regime in chicken embryos. Whether the dosages ever used had satisfied this requirement, we explored by mathematical analysis. A diffusion-in-egg model was established to describe several medicinal diffusions in egg white that involved the instantaneous transient kinetic behavior, the diffusion of medicines in capping volume (the volume from the air sac to the interface of egg yolk). By reviewing the diffusion of various compounds including the cited and the experimentals in this work, we conclude that all the CAM assays ever cited were performed under diffusion control regime rather than kinetic control, which may bring forth deviations caused by a diversity of constitutes in egg white through various medicine-protein interactions.
Colloids and Surfaces B: Biointerfaces
https://doi.org/10.1016/j.colsurfb.2008.09.020
Chorioallantoic Membrane
Air Sacs
Chorioallantoic membrane (CAM) assay
Diffusion control
Kinetic control
Hsieh, C. L., Peng, C. C., Lin, L. Y., Cheng, Y. T., Chen, K. C., & Peng, R. Y. (2009). Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white. Colloids and Surfaces B: Biointerfaces, 68(1), 74-78. https://doi.org/10.1016/j.colsurfb.2008.09.020
Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white. / Hsieh, Chiu Lan; Peng, Chiung Chi; Lin, Li Yun; Cheng, Yu Ting; Chen, Kuan Chou; Peng, Robert Y.
In: Colloids and Surfaces B: Biointerfaces, Vol. 68, No. 1, 01.01.2009, p. 74-78.
Hsieh, CL, Peng, CC, Lin, LY, Cheng, YT, Chen, KC & Peng, RY 2009, 'Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white', Colloids and Surfaces B: Biointerfaces, vol. 68, no. 1, pp. 74-78. https://doi.org/10.1016/j.colsurfb.2008.09.020
Hsieh CL, Peng CC, Lin LY, Cheng YT, Chen KC, Peng RY. Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white. Colloids and Surfaces B: Biointerfaces. 2009 Jan 1;68(1):74-78. https://doi.org/10.1016/j.colsurfb.2008.09.020
Hsieh, Chiu Lan ; Peng, Chiung Chi ; Lin, Li Yun ; Cheng, Yu Ting ; Chen, Kuan Chou ; Peng, Robert Y. / Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white. In: Colloids and Surfaces B: Biointerfaces. 2009 ; Vol. 68, No. 1. pp. 74-78.
@article{c0dcf760d2ea4fad83148eb1cc3f7200,
title = "Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white",
abstract = "The chorioallantoic membrane (CAM) assays have been intensively used to determine angiogenesis and anti-angiogenesis of medicines. In view of bioactivity, this technique should be performed with kinetic control regime in chicken embryos. Whether the dosages ever used had satisfied this requirement, we explored by mathematical analysis. A diffusion-in-egg model was established to describe several medicinal diffusions in egg white that involved the instantaneous transient kinetic behavior, the diffusion of medicines in capping volume (the volume from the air sac to the interface of egg yolk). By reviewing the diffusion of various compounds including the cited and the experimentals in this work, we conclude that all the CAM assays ever cited were performed under diffusion control regime rather than kinetic control, which may bring forth deviations caused by a diversity of constitutes in egg white through various medicine-protein interactions.",
keywords = "Chorioallantoic membrane (CAM) assay, Diffusion control, Kinetic control",
author = "Hsieh, {Chiu Lan} and Peng, {Chiung Chi} and Lin, {Li Yun} and Cheng, {Yu Ting} and Chen, {Kuan Chou} and Peng, {Robert Y.}",
doi = "10.1016/j.colsurfb.2008.09.020",
journal = "Colloids and Surfaces B: Biointerfaces",
T1 - Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white
AU - Hsieh, Chiu Lan
AU - Peng, Chiung Chi
AU - Lin, Li Yun
AU - Cheng, Yu Ting
AU - Chen, Kuan Chou
AU - Peng, Robert Y.
N2 - The chorioallantoic membrane (CAM) assays have been intensively used to determine angiogenesis and anti-angiogenesis of medicines. In view of bioactivity, this technique should be performed with kinetic control regime in chicken embryos. Whether the dosages ever used had satisfied this requirement, we explored by mathematical analysis. A diffusion-in-egg model was established to describe several medicinal diffusions in egg white that involved the instantaneous transient kinetic behavior, the diffusion of medicines in capping volume (the volume from the air sac to the interface of egg yolk). By reviewing the diffusion of various compounds including the cited and the experimentals in this work, we conclude that all the CAM assays ever cited were performed under diffusion control regime rather than kinetic control, which may bring forth deviations caused by a diversity of constitutes in egg white through various medicine-protein interactions.
AB - The chorioallantoic membrane (CAM) assays have been intensively used to determine angiogenesis and anti-angiogenesis of medicines. In view of bioactivity, this technique should be performed with kinetic control regime in chicken embryos. Whether the dosages ever used had satisfied this requirement, we explored by mathematical analysis. A diffusion-in-egg model was established to describe several medicinal diffusions in egg white that involved the instantaneous transient kinetic behavior, the diffusion of medicines in capping volume (the volume from the air sac to the interface of egg yolk). By reviewing the diffusion of various compounds including the cited and the experimentals in this work, we conclude that all the CAM assays ever cited were performed under diffusion control regime rather than kinetic control, which may bring forth deviations caused by a diversity of constitutes in egg white through various medicine-protein interactions.
KW - Chorioallantoic membrane (CAM) assay
KW - Diffusion control
KW - Kinetic control
U2 - 10.1016/j.colsurfb.2008.09.020
DO - 10.1016/j.colsurfb.2008.09.020
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
10.1016/j.colsurfb.2008.09.020