The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana
The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes... Figueroa, Pablo; Léon, Gabriel; Elorza, Alvaro; Holuigue, Loreto; Araya, Alejandro; Jordana, Xavier 2004-10-13 00:00:00 Mitochondrial respiratory complex II contains four subunits: a flavoprotein (SDH1), an iron-sulphur subunit (SDH2) and two membrane anchor subunits (SDH3 and SDH4). We have found that in Arabidopsis thaliana SDH1 and SDH3 are encoded by two, and SDH4 by one nuclear genes, respectively. All these encoded polypeptides are found to be imported into isolated plant mitochondria. While both SDH1 proteins are highly conserved when compared to their counterparts in other organisms, SDH3 and SDH4 share little similarity with non-plant homologues. Expression of SDH1-1, SDH3 and SDH4 genes was detected in all tissues analysed, with the highest steady-state mRNA levels found in flowers and inflorescences. In contrast, the second SDH1 gene (SDH1-2) is expressed at a low level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals http://www.deepdyve.com/lp/springer-journals/the-four-subunits-of-mitochondrial-respiratory-complex-ii-are-encoded-xB9jOFFlgi
Figueroa, Pablo; Léon, Gabriel; Elorza, Alvaro; Holuigue, Loreto; Araya, Alejandro; Jordana, Xavier
Plant Molecular Biology
, Volume 50 (5) – Oct 13, 2004
/lp/springer_journal/the-four-subunits-of-mitochondrial-respiratory-complex-ii-are-encoded-xB9jOFFlgi
Plant Molecular Biology /
Copyright © 2002 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
Mitochondrial respiratory complex II contains four subunits: a flavoprotein (SDH1), an iron-sulphur subunit (SDH2) and two membrane anchor subunits (SDH3 and SDH4). We have found that in Arabidopsis thaliana SDH1 and SDH3 are encoded by two, and SDH4 by one nuclear genes, respectively. All these encoded polypeptides are found to be imported into isolated plant mitochondria. While both SDH1 proteins are highly conserved when compared to their counterparts in other organisms, SDH3 and SDH4 share little similarity with non-plant homologues. Expression of SDH1-1, SDH3 and SDH4 genes was detected in all tissues analysed, with the highest steady-state mRNA levels found in flowers and inflorescences. In contrast, the second SDH1 gene (SDH1-2) is expressed at a low level.
Plant Molecular Biology – Springer Journals
Progress in understanding structure-function relationships in respiratory complex II
Ackrell, B.A.C.
Multiple losses and transfers to the nucleus of two mitochondrial succinate dehydrogenase genes during angiosperm evolution
Adams, K.L.; Rosenblueth, M.; Qiu, Y-L.; Palmer, J.D.
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S.F.; Madden, T.L.; Schaffer, A.A.; Zhang, J.; Zhang, Z.; Miller, W.; Lipman, D.J.
Current Protocols in Molecular Biology
Ausubel, F.M.; Brent, R.; Kingston, R.E.; Moore, D.D.; Seidman, J.G.; Smith, J.A.; Struhl, K.
Genes encoding the same three subunits of respiratory complex II are present in the mitochondrial DNA of two phylogenetically distant eukaryotes
Burger, G.; Lang, B.F.; Reith, M.; Gray, M.W.
Computational method to predict mitochondrially imported proteins and their transit peptides
Claros, M.G.; Vincens, P.
Structure and regulation of SDH3, the yeast gene encoding the cytochrome b560 subunit of respiratory complex II
Daignan-Fornier, B.; Valens, M.; Lemire, B.; Bolotin-Fukuhara, M.
Isolation of plant mitochondria: general principles and criteria of integrity
Douce, R.; Bourguignon, J.; Brouquisse, R.; Neuburger, M.
Characterization of human SDHC gene encoding one of the integral membrane proteins of succinate-quinone oxidoreductase in mitochondria
Elbehti-Green, A.; Au, H.C.; Mascarello, J.T.; Ream-Robinson, D.; Scheffler, I.E.
Pollen specificity elements reside in 30 bp of the proximal promoters of two pollen-expressed genes
Eyal, Y.; Curie, C.; McCormick, S.
Transfer of rps14 from the mitochondrion to the nucleus in maize implied integration within a gene encoding the iron-sulphur subunit of succinate dehydrogenase and expression by alternative splicing
Figueroa, P.; Gómez, I.; Holuigue, L.; Araya, A.; Jordana, X.
The nuclear-encoded SDH2-RPS14 precursor is proteolytically processed between SDH2 and RPS14 to generate maize mitochondrial RPS14
Figueroa, P.; Holuigue, L.; Araya, A.; Jordana, X.
Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana
Figueroa, P.; León, G.; Elorza, A.; Holuigue, L.; Jordana, X.
Complex II subunit 4 (sdh4) homologous sequences in plant mitochondrial genomes
Giegé, P.; Knoop, V.; Brennicke, A.
Improved method for the isolation of RNA from plant tissues
Logemann, J.; Schell, J.; Willmitzer, L.
PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization
Nakai, K.; Horton, P.
A chinese hamster mutant cell line with a defect in the integral membrane protein CII-3 of complex II of the mitochondrial electron transport chain
Oostveen, F.G.; Au, H.C.; Meijer, P.-J.; Scheffler, I.E.
The Saccharomyces cerevisiae succinate-ubiquinone oxidoreductase. Identification of SDH3P amino acid residues involved in ubiquinone binding
Oyedotun, K.S.; Lemire, B.D.
Isolation and nucleotide sequence of the Saccharomyces cerevisiae gene for the succinate dehydrogenase flavoprotein subunit
Robinson, K.M.; Lemire, B.
Differential expression of two succinate dehydrogenase subunit-B genes and a transition in energy metabolism during the development of the parasitic nematode Haemonchus contortus
Roos, M.H.; Tielens, A.G.M.
Molecular genetics of succinate:quinone oxidoreductase in eukaryotes
Scheffler, I.E.
Mitochondrial targeting peptides in plants
Sjöling, S.; Glaser, E.
Complex mtDNA constitutes an approximate 620-kb insertion on Arabidopsis thaliana chromosome 2: implication of potential sequencing errors caused by large-unit repeats
Stupar, R.M.; Lilly, J.W.; Town, C.D.; Cheng, Z.; Kaul, S.; Buell, C.R.; Jiang, J.
The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
Thompson, J.D.; Gibson, T.J.; Plewniak, F.; Jeanmougin, F.; Higgins, D.G.
Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements
Twell, D.; Yamaguchi, J.; Wing, R.A.; Ushiba, J.; McCormick, S.
The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides
Unseld, M.; Marienfeld, J.R.; Brandt, P.; Brennicke, A.
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli
Wood, D.; Darlison, M.G.; Wilde, R.J.; Guest, J.R.
The quinone-binding site in succinate-ubiquinone reductase from Escherichia coli
Yang, X.; Yu, L.; He, D.; Yu, C.-A.
Promoters of nuclear-encoded respiratory chain complex I genes from Arabidopsis thaliana contain a region essential for anther/pollen-specific expression
Zabaleta, E.; Heiser, V.; Grohmann, L.; Brennicke, A.
Figueroa, P., Léon, G., Elorza, A., Holuigue, L., Araya, A., & Jordana, X. (2004). The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana. Plant Molecular Biology, 50(5), 725-734.
Figueroa, Pablo, Gabriel Léon, Alvaro Elorza, Loreto Holuigue, Alejandro Araya, and Xavier Jordana. "The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana." Plant Molecular Biology 50.5 (2004): 725-734.