M22~ (1)
Petković, Marijana
http://vinar.vin.bg.ac.rs/APP/faces/author.xhtml?author_id=orcid%3A%3A0000-0002-8354-995X&item_offset=0&project_offset=0&sort_by=dc.date.issued
Petković, Marijana (60)
Mechanistic studies of the reactions of transition metal ion complexes with biologically relevant molecules Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites
Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) Photonics of micro and nano structured materials
Istraživanje mehanizma interakcija biološki aktivnih jedinjenja sa biomolekulima Effects of laser radiation and plasma on novel materials in their synthesis, modification, and analysis
ARDITI [M1420-010145-FEDER-000005 -CQM+] ARDITI [M142009-5369-FSE-000002]
Dynamics of nonlinear physicochemical and biochemical systems with modeling and predicting of their behavior under nonequilibrium conditions Basic and Clinical Pharmacological research of mechanisms of action and drug interactions in nervous and cardiovascular system
Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications Cellular and molecular basis of neuroinflamation: potential targets for translational medicine and therapy
Portuguese Foundation for Science and Technology European Commission [UIDB/00674/2020] Programa de Cooperacion Territorial INTERREG V-A MAC 2014-2020 through the Project Inv2Mac [MAC2/4.6d/229]
Spanish Ministerio de Economia, Industria y Competividad [RTI2018-099668-BC22] ARDITI [M1420-01-0145-FEDER-000005]
bilateral German-Serbian project - German Academic Exchange Office (Deutscher Akademischer Austauschdienst, DAAD) Bilateral project Republic of Serbia-People's Republic of China [451-00-478/2018-09/16]
CURE [16927] Danube Region Strategies multilateral project -Danube meets Omics [DS 052]
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [59307082] [TRR67] [Projects A8 and Z3] Deutscher Akademischer Austausch Dienst (DAAD)
European Commission [2019093770] European Union REGIONAL COMPETITIVENESS ApliMetaFarma [RC.2.2.08-0046]
Falavarjan Branch, Islamic Azad University, Isfahan University, Abdus Salam International Centre for Theoretical Physics (ICTP) (Trieste, Italy) FCT-Fundacao para a Ciencia e a Tecnologia (CQM Base Fund) [UIDB/00674/2020, ARDITI-CQM_2019-018-ISG (MP), M1420-01-0145FEDER-000005]
Fudan University-CIOMP Joint Fund [FC2017-007] Fudan University-CIOMP Joint Fund [FC2018-001]
German Academic Exchange Service German Research Council [Schi 476/5-1], Federal Ministry of Education and Research [0313836]
Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts
Nešić, Maja D.; Algarra, Manuel; Soto, Juan; Nenadović, Miloš; Popović, Iva; Petković, Marijana
AU  - Nešić, Maja D.
AU  - Algarra, Manuel
AU  - Soto, Juan
AU  - Nenadović, Miloš
AU  - Popović, Iva
AU  - Petković, Marijana
AB  - Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.
T1  - Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts
SP  - 115463
DO  - 10.1016/j.poly.2021.115463
author = "Nešić, Maja D. and Algarra, Manuel and Soto, Juan and Nenadović, Miloš and Popović, Iva and Petković, Marijana",
abstract = "Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.",
title = "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts",
doi = "10.1016/j.poly.2021.115463"
Nešić, M. D., Algarra, M., Soto, J., Nenadović, M., Popović, I.,& Petković, M.. (2021). Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron, 209, 115463.
https://doi.org/10.1016/j.poly.2021.115463
Nešić MD, Algarra M, Soto J, Nenadović M, Popović I, Petković M. Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron. 2021;209:115463.
doi:10.1016/j.poly.2021.115463 .
Nešić, Maja D., Algarra, Manuel, Soto, Juan, Nenadović, Miloš, Popović, Iva, Petković, Marijana, "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts" in Polyhedron, 209 (2021):115463,
https://doi.org/10.1016/j.poly.2021.115463 . .
Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
Matijević, Milica; Žakula, Jelena; Korićanac, Lela; Radoičić, Marija B.; Liang, Xinyue; Mi, Lan; Filipović Tričković, Jelena G.; Valenta-Šobot, Ana; Stanković, Maja N.; Nakarada, Đura; Mojović, Miloš; Petković, Marijana; Stepić, Milutin; Nešić, Maja D.
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Stepić, Milutin
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical & Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
DO  - 10.1007/s43630-021-00082-2
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical & Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
doi = "10.1007/s43630-021-00082-2"
Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical & Photobiological Sciences, 20(8), 1087-1098.
Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical & Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .
Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical & Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
Houdová, Dominika; Soto, Juan; Castro, Rita; Rodrigues, João; Soledad Pino-González, Ma; Petković, Marijana; Bandosz, Teresa J; Algarra, Manuel
AU  - Houdová, Dominika
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Bandosz, Teresa J
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
DO  - 10.1016/j.jcis.2021.02.004
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
doi = "10.1016/j.jcis.2021.02.004"
Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004
Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .
Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .
Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo
Liang, Xinyue; Xie, Yonghui; Wu, Junxin; Wang, Jing; Petković, Marijana; Stepić, Milutin; Zhao, Jinzhuo; Ma, Jiong; Mi, Lan
AU  - Xie, Yonghui
AU  - Wu, Junxin
AU  - Wang, Jing
AU  - Zhao, Jinzhuo
AU  - Ma, Jiong
AB  - Photodynamic therapy (PDT) is a promising cancer treatment that can be implemented using various agents. The conventional photosensitizer Al (III) phthalocyanine chloride tetrasulfonic acid (Pc) has limitations of selectivity in tumor targeting, low affinity to cancer cells, and low two-photon absorption. This study presents a novel photosensitizer FA-TiO2-Pc, which has the TiO2 nanoparticle conjugated with a tumor targeting agent of folic acid (FA), and Pc. FA-TiO2-Pc possessed high targeted photodynamic therapeutic activity and excellent biocompatibility. This promising photosensitizer showed high therapeutic drug efficiency in vitro at a low concentration dose and short incubation time under one-photon excitation (OPE). In vivo, when treated with a low dose of FA-TiO2-Pc and low light irradiation, the tumor growth was depressed in mice bearing HeLa xenograft tumors with minimal side effects. In addition, the two-photon absorption of FA-TiO2-Pc was also enhanced compared to Pc, proving that FA-TiO2-Pc system has a great potential to be used for the therapy of the folate receptor positive cancer cells in both OPE-PDT and two-photon excitation (TPE)-PDT agents. © 2020 Elsevier B.V.
T2  - Journal of Photochemistry and Photobiology B: Biology
T1  - Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo
DO  - 10.1016/j.jphotobiol.2020.112122
author = "Liang, Xinyue and Xie, Yonghui and Wu, Junxin and Wang, Jing and Petković, Marijana and Stepić, Milutin and Zhao, Jinzhuo and Ma, Jiong and Mi, Lan",
abstract = "Photodynamic therapy (PDT) is a promising cancer treatment that can be implemented using various agents. The conventional photosensitizer Al (III) phthalocyanine chloride tetrasulfonic acid (Pc) has limitations of selectivity in tumor targeting, low affinity to cancer cells, and low two-photon absorption. This study presents a novel photosensitizer FA-TiO2-Pc, which has the TiO2 nanoparticle conjugated with a tumor targeting agent of folic acid (FA), and Pc. FA-TiO2-Pc possessed high targeted photodynamic therapeutic activity and excellent biocompatibility. This promising photosensitizer showed high therapeutic drug efficiency in vitro at a low concentration dose and short incubation time under one-photon excitation (OPE). In vivo, when treated with a low dose of FA-TiO2-Pc and low light irradiation, the tumor growth was depressed in mice bearing HeLa xenograft tumors with minimal side effects. In addition, the two-photon absorption of FA-TiO2-Pc was also enhanced compared to Pc, proving that FA-TiO2-Pc system has a great potential to be used for the therapy of the folate receptor positive cancer cells in both OPE-PDT and two-photon excitation (TPE)-PDT agents. © 2020 Elsevier B.V.",
journal = "Journal of Photochemistry and Photobiology B: Biology",
title = "Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo",
doi = "10.1016/j.jphotobiol.2020.112122"
Liang, X., Xie, Y., Wu, J., Wang, J., Petković, M., Stepić, M., Zhao, J., Ma, J.,& Mi, L.. (2021). Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo. in Journal of Photochemistry and Photobiology B: Biology, 215, 112122.
https://doi.org/10.1016/j.jphotobiol.2020.112122
Liang X, Xie Y, Wu J, Wang J, Petković M, Stepić M, Zhao J, Ma J, Mi L. Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo. in Journal of Photochemistry and Photobiology B: Biology. 2021;215:112122.
doi:10.1016/j.jphotobiol.2020.112122 .
Liang, Xinyue, Xie, Yonghui, Wu, Junxin, Wang, Jing, Petković, Marijana, Stepić, Milutin, Zhao, Jinzhuo, Ma, Jiong, Mi, Lan, "Functional titanium dioxide nanoparticle conjugated with phthalocyanine and folic acid as a promising photosensitizer for targeted photodynamic therapy in vitro and in vivo" in Journal of Photochemistry and Photobiology B: Biology, 215 (2021):112122,
https://doi.org/10.1016/j.jphotobiol.2020.112122 . .
Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
Miletić Vukajlović, Jadranka; Drakulić, Dunja R.; Pejić, Snežana; Ilić, Tihomir V.; Stefanović, Aleksandra; Petković, Marijana; Schiller, Jürgen
AU  - Miletić Vukajlović, Jadranka
AU  - Drakulić, Dunja R.
AU  - Pejić, Snežana
AU  - Ilić, Tihomir V.
AU  - Stefanović, Aleksandra
AU  - Schiller, Jürgen
AB  - Rationale: Changes in lipid composition might be associated with the onset and progression of various neurodegenerative diseases. Herein, we investigated the changes in the plasma phosphatidylcholine (PC)/lysophosphatidylcholine (LPC) ratios in patients with Parkinson's disease (PD) in comparison with healthy subjects and their correlation with clinico-pathological features. Methods: The study included 10 controls and 25 patients with PD. All patients were assigned to groups based on clinico-pathological characteristics (gender, age at examination, duration of disease and Hoehn and Yahr (H&Y) stage). The analysis of the PC/LPC intensity ratios in plasma lipid extracts was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: PD patients exhibited an increased PC/LPC intensity ratio in comparison with the control group of healthy subjects. Furthermore, the investigated ratio was shown to be correlated with clinico-pathological parameters, in particular with H&Y stage and disease duration. The PC/LPC intensity ratio in plasma samples of PD patients was found to be elevated in all examined H&Y stages and throughout the disease duration. Conclusions: To our knowledge, this is the first study examining the PC/LPC ratios in plasma of patients with PD and illustrating their correlation with clinico-pathological features. Although the presented results may be considered as preliminary due to the limited number of participants, the observed alterations of PC/LPC ratios in plasma might be a first step in the characterization of plasma lipid changes in PD patients and an indicator of lipid reconfiguration. © 2019 John Wiley & Sons, Ltd.
T2  - Rapid Communications in Mass Spectrometry
T1  - Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
DO  - 10.1002/rcm.8595
author = "Miletić Vukajlović, Jadranka and Drakulić, Dunja R. and Pejić, Snežana and Ilić, Tihomir V. and Stefanović, Aleksandra and Petković, Marijana and Schiller, Jürgen",
abstract = "Rationale: Changes in lipid composition might be associated with the onset and progression of various neurodegenerative diseases. Herein, we investigated the changes in the plasma phosphatidylcholine (PC)/lysophosphatidylcholine (LPC) ratios in patients with Parkinson's disease (PD) in comparison with healthy subjects and their correlation with clinico-pathological features. Methods: The study included 10 controls and 25 patients with PD. All patients were assigned to groups based on clinico-pathological characteristics (gender, age at examination, duration of disease and Hoehn and Yahr (H&Y) stage). The analysis of the PC/LPC intensity ratios in plasma lipid extracts was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: PD patients exhibited an increased PC/LPC intensity ratio in comparison with the control group of healthy subjects. Furthermore, the investigated ratio was shown to be correlated with clinico-pathological parameters, in particular with H&Y stage and disease duration. The PC/LPC intensity ratio in plasma samples of PD patients was found to be elevated in all examined H&Y stages and throughout the disease duration. Conclusions: To our knowledge, this is the first study examining the PC/LPC ratios in plasma of patients with PD and illustrating their correlation with clinico-pathological features. Although the presented results may be considered as preliminary due to the limited number of participants, the observed alterations of PC/LPC ratios in plasma might be a first step in the characterization of plasma lipid changes in PD patients and an indicator of lipid reconfiguration. © 2019 John Wiley & Sons, Ltd.",
journal = "Rapid Communications in Mass Spectrometry",
title = "Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease",
doi = "10.1002/rcm.8595"
Miletić Vukajlović, J., Drakulić, D. R., Pejić, S., Ilić, T. V., Stefanović, A., Petković, M.,& Schiller, J.. (2020). Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease. in Rapid Communications in Mass Spectrometry, 34(4).
https://doi.org/10.1002/rcm.8595
Miletić Vukajlović J, Drakulić DR, Pejić S, Ilić TV, Stefanović A, Petković M, Schiller J. Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease. in Rapid Communications in Mass Spectrometry. 2020;34(4).
doi:10.1002/rcm.8595 .
Miletić Vukajlović, Jadranka, Drakulić, Dunja R., Pejić, Snežana, Ilić, Tihomir V., Stefanović, Aleksandra, Petković, Marijana, Schiller, Jürgen, "Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease" in Rapid Communications in Mass Spectrometry, 34, no. 4 (2020),
https://doi.org/10.1002/rcm.8595 . .
Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate
Petković, Marijana; Leopold, Jenny; Popović, Iva A.; Dimić, Dušan; Ilić, Jelica; Nenadović, Miloš; Rakočević, Zlatko Lj.; Schiller, Jürgen
AU  - Leopold, Jenny
AU  - Popović, Iva A.
AU  - Dimić, Dušan
AU  - Ilić, Jelica
AU  - Rakočević, Zlatko Lj.
AB  - In this work, the performances of two ionic liquid matrices (ILMs) with the same ammonium counterpart for mass spectrometric analysis of the insoluble and soluble sucralfate were compared. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was performed assisted by the butylammonium salts of α-cyano-hydroxycinnamic acid (CHCAB) and 2,5-dihydroxybenzoic acid (DHBB). CHCAB has a higher IE than DHBB, but better optical properties. CHCAB is more suitable for the analysis of sucralfate, although molecular ions of both compounds were detectable only with low intensities. Thus, optical properties of ILMs are crucial to enhance the sensitivity of MALDI MS detection of polysulfated oligosaccharides. © 2019, © 2019 Taylor & Francis Group, LLC.
T2  - Journal of Carbohydrate Chemistry
T1  - Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate
author = "Petković, Marijana and Leopold, Jenny and Popović, Iva A. and Dimić, Dušan and Ilić, Jelica and Nenadović, Miloš and Rakočević, Zlatko Lj. and Schiller, Jürgen",
abstract = "In this work, the performances of two ionic liquid matrices (ILMs) with the same ammonium counterpart for mass spectrometric analysis of the insoluble and soluble sucralfate were compared. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) was performed assisted by the butylammonium salts of α-cyano-hydroxycinnamic acid (CHCAB) and 2,5-dihydroxybenzoic acid (DHBB). CHCAB has a higher IE than DHBB, but better optical properties. CHCAB is more suitable for the analysis of sucralfate, although molecular ions of both compounds were detectable only with low intensities. Thus, optical properties of ILMs are crucial to enhance the sensitivity of MALDI MS detection of polysulfated oligosaccharides. © 2019, © 2019 Taylor & Francis Group, LLC.",
journal = "Journal of Carbohydrate Chemistry",
title = "Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate",
doi = "10.1080/07328303.2019.1669633"
Petković, M., Leopold, J., Popović, I. A., Dimić, D., Ilić, J., Nenadović, M., Rakočević, Z. Lj.,& Schiller, J.. (2020). Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate. in Journal of Carbohydrate Chemistry, 39(1), 1-23.
Petković M, Leopold J, Popović IA, Dimić D, Ilić J, Nenadović M, Rakočević ZL, Schiller J. Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate. in Journal of Carbohydrate Chemistry. 2020;39(1):1-23.
doi:10.1080/07328303.2019.1669633 .
Petković, Marijana, Leopold, Jenny, Popović, Iva A., Dimić, Dušan, Ilić, Jelica, Nenadović, Miloš, Rakočević, Zlatko Lj., Schiller, Jürgen, "Performances of ionic liquid matrices with butyl ammonium counterion for matrix-assisted laser desorption/ionization mass spectrometric detection and analysis of sucralfate" in Journal of Carbohydrate Chemistry, 39, no. 1 (2020):1-23,
https://doi.org/10.1080/07328303.2019.1669633 . .
The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer
Ivanović, Marija; Kljajević, Ljiljana M.; Gulicovski, Jelena; Petković, Marijana; Janković-Častvan, Ivona; Bučevac, Dušan; Nenadović, Snežana S.
AU  - Ivanović, Marija
AU  - Kljajević, Ljiljana M.
AU  - Gulicovski, Jelena
AU  - Janković-Častvan, Ivona
AU  - Bučevac, Dušan
AU  - Nenadović, Snežana S.
AB  - This paper outlines the production of an inorganic polymer/geopolymer using a metakaolin by an environmental friendly, energy saving, clean technology to conserve natural environment and resources. The influence of alkali activation, i.e. different concentration of NaOH as a component of alkali activator mixture on the process of geopolymerization of metakaolin is investigated. Also, process of aging time of geopolymer is followed by several analytical methods. The structure of metakaolin and metakaolin based geopolymers and their physicochemical properties were studied using X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FTIR) and after 28 days scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) was applied for the surface characterization of the samples. A shift of the Si-O or Si-O-X (X=Al, Si, Na...) bands as the molarity of activator increasing during geopolymerization process was observed by FTIR. Mass spectra of geopolymers were characterized by MALDI TOF mass spectrometer. Structural reorganization of geopolymer samples occurs during the curing/aging in accordance with a geopolymerization mechanism.
T1  - The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer
DO  - 10.2298/SOS2002219I
author = "Ivanović, Marija and Kljajević, Ljiljana M. and Gulicovski, Jelena and Petković, Marijana and Janković-Častvan, Ivona and Bučevac, Dušan and Nenadović, Snežana S.",
abstract = "This paper outlines the production of an inorganic polymer/geopolymer using a metakaolin by an environmental friendly, energy saving, clean technology to conserve natural environment and resources. The influence of alkali activation, i.e. different concentration of NaOH as a component of alkali activator mixture on the process of geopolymerization of metakaolin is investigated. Also, process of aging time of geopolymer is followed by several analytical methods. The structure of metakaolin and metakaolin based geopolymers and their physicochemical properties were studied using X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FTIR) and after 28 days scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) was applied for the surface characterization of the samples. A shift of the Si-O or Si-O-X (X=Al, Si, Na...) bands as the molarity of activator increasing during geopolymerization process was observed by FTIR. Mass spectra of geopolymers were characterized by MALDI TOF mass spectrometer. Structural reorganization of geopolymer samples occurs during the curing/aging in accordance with a geopolymerization mechanism.",
title = "The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer",
doi = "10.2298/SOS2002219I"
Ivanović, M., Kljajević, L. M., Gulicovski, J., Petković, M., Janković-Častvan, I., Bučevac, D.,& Nenadović, S. S.. (2020). The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer. in Science of Sintering, 52(2), 219-229.
https://doi.org/10.2298/SOS2002219I
Ivanović M, Kljajević LM, Gulicovski J, Petković M, Janković-Častvan I, Bučevac D, Nenadović SS. The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer. in Science of Sintering. 2020;52(2):219-229.
doi:10.2298/SOS2002219I .
Ivanović, Marija, Kljajević, Ljiljana M., Gulicovski, Jelena, Petković, Marijana, Janković-Častvan, Ivona, Bučevac, Dušan, Nenadović, Snežana S., "The effect of the concentration of alkaline activator and aging time on the structure of metakaolin based geopolymer" in Science of Sintering, 52, no. 2 (2020):219-229,
https://doi.org/10.2298/SOS2002219I . .
SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
Nešić, Maja D.; Dučić, Tanja; Liang, Xinyue; Algarra, Manuel; Mi, Lan; Korićanac, Lela; Žakula, Jelena; Kop, Tatjana J.; Bjelaković, Mira S.; Mitrović, Aleksandra; Gojgić Cvijović, Gordana D.; Stepić, Milutin; Petković, Marijana
AU  - Dučić, Tanja
AU  - Kop, Tatjana J.
AU  - Bjelaković, Mira S.
AU  - Mitrović, Aleksandra
AU  - Gojgić Cvijović, Gordana D.
AB  - Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.
T2  - International Journal of Biological Macromolecules
T1  - SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives
DO  - 10.1016/j.ijbiomac.2020.10.141
author = "Nešić, Maja D. and Dučić, Tanja and Liang, Xinyue and Algarra, Manuel and Mi, Lan and Korićanac, Lela and Žakula, Jelena and Kop, Tatjana J. and Bjelaković, Mira S. and Mitrović, Aleksandra and Gojgić Cvijović, Gordana D. and Stepić, Milutin and Petković, Marijana",
abstract = "Objects of the present study are improved fullerene C60 drug carrier properties trough encapsulation by microbial polysaccharides, levan (LEV), pullulan (PUL), and their hydrophobized cholesterol-derivatives (CHL and CHP), that show better interaction with cancer cells. The zeta potential, polydispersity index, and the diameter of particles were determined, and their cytotoxicity against three cancer cell lines were tested. Biochemical changes in HeLa cells are analyzed by synchrotron radiation (SR) FTIR spectro-microscopy combined with the principal component analysis (PCA). The most significant changes occur in HeLa cells treated with LEV-C60 and correspond to the changes in the protein region, i.e. Amide I band, and the changes in the structure of lipid bodies and membrane fluidity are evident. The highest cytotoxicity was also induced by LEV-C60. In HeLa cells, cytotoxicity could not be strictly associated with biochemical changes in lipids, proteins and nucleic acids, but these findings are significant contribution to the study of the mechanism of interaction of C60-based nanoparticles with cellular biomolecules. In conclusion, LEV, PUL, CHL, and CHP enhanced fullerene C60 potential to be used as target drug delivery system with the ability to induce specific intracellular changes in HeLa cancer cells.",
journal = "International Journal of Biological Macromolecules",
title = "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives",
doi = "10.1016/j.ijbiomac.2020.10.141"
Nešić, M. D., Dučić, T., Liang, X., Algarra, M., Mi, L., Korićanac, L., Žakula, J., Kop, T. J., Bjelaković, M. S., Mitrović, A., Gojgić Cvijović, G. D., Stepić, M.,& Petković, M.. (2020). SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules, 165, 2541-2549.
https://doi.org/10.1016/j.ijbiomac.2020.10.141
Nešić MD, Dučić T, Liang X, Algarra M, Mi L, Korićanac L, Žakula J, Kop TJ, Bjelaković MS, Mitrović A, Gojgić Cvijović GD, Stepić M, Petković M. SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives. in International Journal of Biological Macromolecules. 2020;165:2541-2549.
doi:10.1016/j.ijbiomac.2020.10.141 .
Nešić, Maja D., Dučić, Tanja, Liang, Xinyue, Algarra, Manuel, Mi, Lan, Korićanac, Lela, Žakula, Jelena, Kop, Tatjana J., Bjelaković, Mira S., Mitrović, Aleksandra, Gojgić Cvijović, Gordana D., Stepić, Milutin, Petković, Marijana, "SR-FTIR spectro-microscopic interaction study of biochemical changes in HeLa cells induced by Levan-C60, Pullulan-C60, and their cholesterol-derivatives" in International Journal of Biological Macromolecules, 165 (2020):2541-2549,
https://doi.org/10.1016/j.ijbiomac.2020.10.141 . .
Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer
Matijević, Milica; Nakarada, Đura; Liang, Xinyue; Korićanac, Lela; Rajsiglova, Lenka; Vannucci, Luca; Nešić, Maja D.; Vranješ, Mila; Mojović, Miloš D.; Mi, Lan; Estrela-Lopis, Irina; Böttner, Julia; Šaponjić, Zoran; Petković, Marijana; Stepić, Milutin
AU  - Rajsiglova, Lenka
AU  - Vannucci, Luca
AU  - Vranješ, Mila
AU  - Mojović, Miloš D.
AU  - Estrela-Lopis, Irina
AU  - Böttner, Julia
AU  - Šaponjić, Zoran
AB  - TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells' viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines' metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study.
T2  - Journal of Nanoparticle Research
T1  - Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer
author = "Matijević, Milica and Nakarada, Đura and Liang, Xinyue and Korićanac, Lela and Rajsiglova, Lenka and Vannucci, Luca and Nešić, Maja D. and Vranješ, Mila and Mojović, Miloš D. and Mi, Lan and Estrela-Lopis, Irina and Böttner, Julia and Šaponjić, Zoran and Petković, Marijana and Stepić, Milutin",
abstract = "TiO2 prolatenanospheroids (PNSs) may be photosensitizers (PSs), which act by catalyzation of hydroxyl radical (∙OH) formation upon light illumination. ∙OH might, in turn, contribute to killing of cancer cells. On the other hand, there is great concern about toxicity in the dark of TiO2 nanoparticles in general. In this work, we have investigated the biocompatibility of TiO2 PNSs of the anatase crystal form (length between 100 and 300 nm and width 50 nm) in the dark with immune cells and light-induced cytotoxicity on several cancer cell lines. The effects of the treatment of different cell lines with several concentrations of TiO2 PNSs suspensions showed the specifics of cells' viability and the intracellular localization. The results of in vitro studies obtained by cytotoxicity assays adjusted to individual cell lines' metabolism point towards the biocompatibility of TiO2 PNSs at low and moderate concentrations in the dark, which neither kill the cells, nor induce activation of the immune system cells. Laser scanning confocal microscopy revealed that PNSs are taken up by cells, and insight into the intracellular distribution was obtained in this study.",
journal = "Journal of Nanoparticle Research",
title = "Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer",
Matijević, M., Nakarada, Đ., Liang, X., Korićanac, L., Rajsiglova, L., Vannucci, L., Nešić, M. D., Vranješ, M., Mojović, M. D., Mi, L., Estrela-Lopis, I., Böttner, J., Šaponjić, Z., Petković, M.,& Stepić, M.. (2020). Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer. in Journal of Nanoparticle Research, 22(7), 175.
Matijević M, Nakarada Đ, Liang X, Korićanac L, Rajsiglova L, Vannucci L, Nešić MD, Vranješ M, Mojović MD, Mi L, Estrela-Lopis I, Böttner J, Šaponjić Z, Petković M, Stepić M. Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer. in Journal of Nanoparticle Research. 2020;22(7):175.
Matijević, Milica, Nakarada, Đura, Liang, Xinyue, Korićanac, Lela, Rajsiglova, Lenka, Vannucci, Luca, Nešić, Maja D., Vranješ, Mila, Mojović, Miloš D., Mi, Lan, Estrela-Lopis, Irina, Böttner, Julia, Šaponjić, Zoran, Petković, Marijana, Stepić, Milutin, "Biocompatibility of TiO2 prolate nanospheroids as a potential photosenzitizer in therapy of cancer" in Journal of Nanoparticle Research, 22, no. 7 (2020):175,
Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes
Radisavljević, Snežana; Ćoćić, Dušan; Jovanović, Snežana; Šmit, Biljana; Petković, Marijana; Milivojević, Nevena; Planojević, Nevena; Marković, Snežana D.; Petrović, Biljana V.
AU  - Radisavljević, Snežana
AU  - Ćoćić, Dušan
AU  - Jovanović, Snežana
AU  - Šmit, Biljana
AU  - Milivojević, Nevena
AU  - Planojević, Nevena
AU  - Marković, Snežana D.
AU  - Petrović, Biljana V.
AB  - Abstract: In this study, we have synthesized a series of dinuclear and trinuclear gold(III) complexes of the general formula [Au2(N–N)Cl6] (1–3) for dinuclear and [Au3(N–N)2Cl8]+ (4–6) for trinuclear compounds, respectively, in which N–N is a bidentate ligand (1,4-diaminobutane; 1,6-diaminohexane or 1,8-diaminooctane). These complexes were characterized by elemental analysis, molar conductivity, and spectroscopic techniques (IR, UV–Vis, 1H NMR, ESI–MS). We performed DFT calculations to get insight into the geometry of the studies complexes. DNA-binding studies were performed by UV–Vis spectrophotometry and fluorescence spectroscopy. The results of competitive reactions between gold(III) complexes and ethidium bromide (EB) towards DNA have shown that selected complexes can displace EB from DNA-EB adduct. In addition, these experiments confirm that polynuclear gold(III) complexes interact with DNA covalently or via intercalation. Furthermore, high values of binding constants of gold(III) complexes towards bovine serum albumin (BSA) protein indicate good binding affinity. In addition, redox stability of complexes in the presence of DNA/BSA was confirmed by cyclic voltammetry. Results of the interactions between gold(III) complexes with DNA/BSA were discussed in reference to molecular docking data obtain by Molegro virtual docker. The cytotoxic activity of synthesized gold(III) complexes was evaluated on human breast cancer cell line (MDA-MB-231), human colorectal cancer cell line (HCT-116), and normal human lung fibroblast cell line (MRC-5). All complexes dose-dependently reduced cancer and normal cells viabilities, with significant cytotoxic effects (IC50 < 25 μM) for trinuclear gold(III) complexes (4, 5) on HCT-116 cells. Graphic abstract: [Figure not available: see fulltext.].
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes
author = "Radisavljević, Snežana and Ćoćić, Dušan and Jovanović, Snežana and Šmit, Biljana and Petković, Marijana and Milivojević, Nevena and Planojević, Nevena and Marković, Snežana D. and Petrović, Biljana V.",
abstract = "Abstract: In this study, we have synthesized a series of dinuclear and trinuclear gold(III) complexes of the general formula [Au2(N–N)Cl6] (1–3) for dinuclear and [Au3(N–N)2Cl8]+ (4–6) for trinuclear compounds, respectively, in which N–N is a bidentate ligand (1,4-diaminobutane; 1,6-diaminohexane or 1,8-diaminooctane). These complexes were characterized by elemental analysis, molar conductivity, and spectroscopic techniques (IR, UV–Vis, 1H NMR, ESI–MS). We performed DFT calculations to get insight into the geometry of the studies complexes. DNA-binding studies were performed by UV–Vis spectrophotometry and fluorescence spectroscopy. The results of competitive reactions between gold(III) complexes and ethidium bromide (EB) towards DNA have shown that selected complexes can displace EB from DNA-EB adduct. In addition, these experiments confirm that polynuclear gold(III) complexes interact with DNA covalently or via intercalation. Furthermore, high values of binding constants of gold(III) complexes towards bovine serum albumin (BSA) protein indicate good binding affinity. In addition, redox stability of complexes in the presence of DNA/BSA was confirmed by cyclic voltammetry. Results of the interactions between gold(III) complexes with DNA/BSA were discussed in reference to molecular docking data obtain by Molegro virtual docker. The cytotoxic activity of synthesized gold(III) complexes was evaluated on human breast cancer cell line (MDA-MB-231), human colorectal cancer cell line (HCT-116), and normal human lung fibroblast cell line (MRC-5). All complexes dose-dependently reduced cancer and normal cells viabilities, with significant cytotoxic effects (IC50 < 25 μM) for trinuclear gold(III) complexes (4, 5) on HCT-116 cells. Graphic abstract: [Figure not available: see fulltext.].",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes",
Radisavljević, S., Ćoćić, D., Jovanović, S., Šmit, B., Petković, M., Milivojević, N., Planojević, N., Marković, S. D.,& Petrović, B. V.. (2019). Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes. in JBIC Journal of Biological Inorganic Chemistry, 24(7), 1057-1076.
Radisavljević S, Ćoćić D, Jovanović S, Šmit B, Petković M, Milivojević N, Planojević N, Marković SD, Petrović BV. Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes. in JBIC Journal of Biological Inorganic Chemistry. 2019;24(7):1057-1076.
Radisavljević, Snežana, Ćoćić, Dušan, Jovanović, Snežana, Šmit, Biljana, Petković, Marijana, Milivojević, Nevena, Planojević, Nevena, Marković, Snežana D., Petrović, Biljana V., "Synthesis, characterization, DFT study, DNA/BSA-binding affinity, and cytotoxicity of some dinuclear and trinuclear gold(III) complexes" in JBIC Journal of Biological Inorganic Chemistry, 24, no. 7 (2019):1057-1076,
Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
Nišavić, Marija; Janjić, Goran V.; Hozić, Amela; Petković, Marijana; Milčić, Miloš K.; Vujčić, Zoran; Cindrić, Mario
AU  - Nišavić, Marija
AU  - Janjić, Goran V.
AU  - Hozić, Amela
AU  - Milčić, Miloš K.
AU  - Vujčić, Zoran
AU  - Cindrić, Mario
UR  - http://xlink.rsc.org/?DOI=C7MT00330G
AB  - Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.
T2  - Metallomics
T1  - Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins
DO  - 10.1039/C7MT00330G
author = "Nišavić, Marija and Janjić, Goran V. and Hozić, Amela and Petković, Marijana and Milčić, Miloš K. and Vujčić, Zoran and Cindrić, Mario",
abstract = "Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4-chloro-2,2:6,2-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode. The negative ion mode was achieved after the post-column addition of an isopropanol solution of formaldehyde that enabled sample ionization at micro-flow rates. The negative ion mode MS has been proved to be beneficial for the analysis of binding sites on ruthenated protein in terms of ion charge reduction and consequent simplification of target sequence identification based on isotopic differences between ruthenated and non-ruthenated peptides. Moreover, the negative ion mode ESI MS shows the advantage of singly charged ion formation and, unlike MALDI MS, it does not cause complete ligand fragmentation, merging the benefits of each method into a single experiment. Six target sequences were identified for the binding of en and dach compounds, and four sequences for the binding of bipy. All compounds have been found to bind histidine and one aspartate residue. Docking studies showed that the identified sequences are the constituents of five distinct binding sites for en and dach, or two sites for the bipy complex. The selection of binding sites seems to be dependent on the chelate ligand and the form of the complex prior or after hydrolysis of the leaving chloride ligand.",
journal = "Metallomics",
title = "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins",
doi = "10.1039/C7MT00330G"
Nišavić, M., Janjić, G. V., Hozić, A., Petković, M., Milčić, M. K., Vujčić, Z.,& Cindrić, M.. (2018). Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics, 10(4), 587-594.
https://doi.org/10.1039/C7MT00330G
Nišavić M, Janjić GV, Hozić A, Petković M, Milčić MK, Vujčić Z, Cindrić M. Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins. in Metallomics. 2018;10(4):587-594.
doi:10.1039/C7MT00330G .
Nišavić, Marija, Janjić, Goran V., Hozić, Amela, Petković, Marijana, Milčić, Miloš K., Vujčić, Zoran, Cindrić, Mario, "Positive and negative nano-electrospray mass spectrometry of ruthenated serum albumin supported by docking studies: an integrated approach towards defining metallodrug binding sites on proteins" in Metallomics, 10, no. 4 (2018):587-594,
https://doi.org/10.1039/C7MT00330G . .
Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
Miletić, Jadranka; Drakulić, Dunja R.; Pejić, Snežana; Petković, Marijana; Ilić, Tihomir V.; Miljković, Milica; Stefanović, Aleksandra; Prostran, Milica; Stojanov, Marina
AU  - Miletić, Jadranka
AU  - Miljković, Milica
AU  - Prostran, Milica
AU  - Stojanov, Marina
AB  - Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.
T2  - International Journal of Neuroscience
T1  - Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease
author = "Miletić, Jadranka and Drakulić, Dunja R. and Pejić, Snežana and Petković, Marijana and Ilić, Tihomir V. and Miljković, Milica and Stefanović, Aleksandra and Prostran, Milica and Stojanov, Marina",
abstract = "Background: Biomarkers of oxidative stress are relevant in the evaluation of the disease status and prooxidant-antioxidant balance, advanced oxidation protein products and lipid peroxidation products (malondialdehyde and 4-hydroxynonenal) are being extensively evaluated regarding their relationship with clinical presentation and disease severity.Aim of the study: The aim of this study was to evaluate the levels of the above-mentioned parameters in plasma of 39 men and 17 women with Parkinson's disease, originated from the Republic of Serbia and their relation to clinicopathological characteristics (gender, age at examination, duration of the disease, and Hoehn and Yahr score) and oxidative status.Results: The incidence of disease was 2:1 towards males. The investigated oxidative parameters were gender and Hoehn and Yahr related. Significant association of higher Hoehn and Yahr scores was observed for malondialdehyde (p = 0.01) and prooxidant-antioxidant balance (p = 0.02). Relation between oxidant-antioxidant status was further supported by observed positive correlation between 4-hydroxynonenal (p = 0.04) and prooxidant-antioxidant balance (p = 0.03). Finally, the multivariate analysis indicated that prooxidant-antioxidant balance and malondialdehyde were partially determined by gender (10.6% and 7.6%) and Hoehn and Yahr scores (13.6% and 18.8%), while Hoehn and Yahr scores contributed to the variance of advanced oxidation protein products with 13.2%.Conclusion: Our results indicate the higher level of oxidative stress (oxidant-antioxidant imbalance) and possible relation of several markers with gender and disease stage in patients with Parkinson's disease. The analyzed markers could be used to specify the severity of oxidative stress; however, their potential value should be analyzed in further studies.",
journal = "International Journal of Neuroscience",
title = "Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease",
Miletić, J., Drakulić, D. R., Pejić, S., Petković, M., Ilić, T. V., Miljković, M., Stefanović, A., Prostran, M.,& Stojanov, M.. (2018). Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience, 128(7), 600-607.
Miletić J, Drakulić DR, Pejić S, Petković M, Ilić TV, Miljković M, Stefanović A, Prostran M, Stojanov M. Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease. in International Journal of Neuroscience. 2018;128(7):600-607.
Miletić, Jadranka, Drakulić, Dunja R., Pejić, Snežana, Petković, Marijana, Ilić, Tihomir V., Miljković, Milica, Stefanović, Aleksandra, Prostran, Milica, Stojanov, Marina, "Prooxidant–antioxidant balance, advanced oxidation protein products and lipid peroxidation in Serbian patients with Parkinson's disease" in International Journal of Neuroscience, 128, no. 7 (2018):600-607,
Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy
Matijević, Milica; Nešić, Maja A.; Stepić, Milutin; Radoičić, Marija B.; Šaponjić, Zoran; Petković, Marijana
AU  - Nešić, Maja A.
UR  - http://link.springer.com/10.1007/s11082-018-1495-z
AB  - Photodynamic therapy implies a combined use of a photosensitizing medicament and low-intensity light to cause selective damage to the target tissue—tumor. As potential medicament, we use Ru(II)(dcbpy)2Cl2complex, and in order to achieve better photosensitization properties, the Ru complex was attached to the nano carrier—TiO2nanoparticles. Additionally, this nanocomposite system was encapsulated in the phospholipid vesicles, which could be classified as small unilamellar vesicles, based on the technique of production. The complex-release tests were performed under light illumination, at pH 5, characteristic for tumor cells` interior and compared with the release pattern at pH 7, characteristic for the serum, i.e. physiological solution.
T2  - Optical and Quantum Electronics
T1  - Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy
author = "Matijević, Milica and Nešić, Maja A. and Stepić, Milutin and Radoičić, Marija B. and Šaponjić, Zoran and Petković, Marijana",
abstract = "Photodynamic therapy implies a combined use of a photosensitizing medicament and low-intensity light to cause selective damage to the target tissue—tumor. As potential medicament, we use Ru(II)(dcbpy)2Cl2complex, and in order to achieve better photosensitization properties, the Ru complex was attached to the nano carrier—TiO2nanoparticles. Additionally, this nanocomposite system was encapsulated in the phospholipid vesicles, which could be classified as small unilamellar vesicles, based on the technique of production. The complex-release tests were performed under light illumination, at pH 5, characteristic for tumor cells` interior and compared with the release pattern at pH 7, characteristic for the serum, i.e. physiological solution.",
journal = "Optical and Quantum Electronics",
title = "Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy",
Matijević, M., Nešić, M. A., Stepić, M., Radoičić, M. B., Šaponjić, Z.,& Petković, M.. (2018). Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy. in Optical and Quantum Electronics, 50(6), 232.
Matijević M, Nešić MA, Stepić M, Radoičić MB, Šaponjić Z, Petković M. Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy. in Optical and Quantum Electronics. 2018;50(6):232.
Matijević, Milica, Nešić, Maja A., Stepić, Milutin, Radoičić, Marija B., Šaponjić, Zoran, Petković, Marijana, "Light controllable TiO2-Ru nanocomposite system encapsulated in phospholipid unilamellar vesicles for anti-cancer photodynamic therapy" in Optical and Quantum Electronics, 50, no. 6 (2018):232,
Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics
Matijević, Milica; Popović, Iva A.; Stepić, Milutin; Nešić, Maja D.; Radoičić, Marija B.; Stanković, Maja; Šaponjić, Zoran; Petković, Marijana
AU  - Stanković, Maja
AB  - Among various nano-scaled materials composed from a spectrum of chemical compounds, inorganic nanoparticles are very attractive due to their physico-chemical properties, as well as their availability, simplicity, possibility of modifications, stability and biocompatibility. They are, on the one hand, an useful tool in advanced analytical chemistry, in particular for studying of biologically-relevant processes, but also important as functional parts of the systems designed for controlled and targeted delivery of medicaments for treatment of a variety of diseases and for imaging. So far, thousands of compounds and systems have been developed for the above-mentioned purposes, but there are only a few reviews dealing with these topics. The aim of this review is, thus, to summarize recent applications of nano-structured inorganic materials in the field of drug delivery, bioimaging and bioanalytics, and to give a prospective from the standpoint of biology-related applications.
T2  - Biologica Nyssana
T1  - Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics
DO  - 10.5281/zenodo.1470841
author = "Matijević, Milica and Popović, Iva A. and Stepić, Milutin and Nešić, Maja D. and Radoičić, Marija B. and Stanković, Maja and Šaponjić, Zoran and Petković, Marijana",
abstract = "Among various nano-scaled materials composed from a spectrum of chemical compounds, inorganic nanoparticles are very attractive due to their physico-chemical properties, as well as their availability, simplicity, possibility of modifications, stability and biocompatibility. They are, on the one hand, an useful tool in advanced analytical chemistry, in particular for studying of biologically-relevant processes, but also important as functional parts of the systems designed for controlled and targeted delivery of medicaments for treatment of a variety of diseases and for imaging. So far, thousands of compounds and systems have been developed for the above-mentioned purposes, but there are only a few reviews dealing with these topics. The aim of this review is, thus, to summarize recent applications of nano-structured inorganic materials in the field of drug delivery, bioimaging and bioanalytics, and to give a prospective from the standpoint of biology-related applications.",
journal = "Biologica Nyssana",
title = "Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics",
doi = "10.5281/zenodo.1470841"
Matijević, M., Popović, I. A., Stepić, M., Nešić, M. D., Radoičić, M. B., Stanković, M., Šaponjić, Z.,& Petković, M.. (2018). Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics. in Biologica Nyssana, 9(1), 1-19.
Matijević M, Popović IA, Stepić M, Nešić MD, Radoičić MB, Stanković M, Šaponjić Z, Petković M. Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics. in Biologica Nyssana. 2018;9(1):1-19.
doi:10.5281/zenodo.1470841 .
Matijević, Milica, Popović, Iva A., Stepić, Milutin, Nešić, Maja D., Radoičić, Marija B., Stanković, Maja, Šaponjić, Zoran, Petković, Marijana, "Inorganic Nanoparticles In Biology: Drug Carriers And Auxiliary Tools In Bioimaging And Bioanalytics" in Biologica Nyssana, 9, no. 1 (2018):1-19,
https://doi.org/10.5281/zenodo.1470841 . .
Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes
Matijević, Milica; Cindrić, Marina; Petković, Marijana; Nišavić, Marija
AU  - Cindrić, Marina
C3  - FEBS OPEN BIO
T1  - Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes
SP  - 406+
DO  - 10.1002/2211-5463.12453
author = "Matijević, Milica and Cindrić, Marina and Petković, Marijana and Nišavić, Marija",
journal = "FEBS OPEN BIO",
title = "Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes",
pages = "406+",
doi = "10.1002/2211-5463.12453"
Matijević, M., Cindrić, M., Petković, M.,& Nišavić, M.. (2018). Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes. in FEBS OPEN BIO, 8(1), 406+.
https://doi.org/10.1002/2211-5463.12453
Matijević M, Cindrić M, Petković M, Nišavić M. Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes. in FEBS OPEN BIO. 2018;8(1):406+.
doi:10.1002/2211-5463.12453 .
Matijević, Milica, Cindrić, Marina, Petković, Marijana, Nišavić, Marija, "Structural characterization of transferrin-bound ruthenium(II) terpyridine complexes" in FEBS OPEN BIO, 8, no. 1 (2018):406+,
https://doi.org/10.1002/2211-5463.12453 . .
Gold chloride cluster ions generated by vacuum laser ablation
Rajčić, Boris; Dimitrijević, Silvana B.; Petković, Marijana; Nišavić, Marija; Cindrić, Mario; Veljković, Filip M.; Veličković, Suzana
AU  - Rajčić, Boris
AU  - Dimitrijević, Silvana B.
AU  - Veljković, Filip M.
AU  - Veličković, Suzana
UR  - http://link.springer.com/10.1007/s11082-018-1476-2
AB  - In this work, a simple way for study the possibility of formation a vapor cluster species of tetrachloroauric acid (HAuCl4), using the laser ablation in the absence of a buffer or reactive atmosphere, and without a postablation supersonic expansion on a commercial matrix assisted laser desorption/ionization time-of-flight mass spectrometer, is reported. Tetrachloroauric acid is known as precursor for the synthesis of gold nanostructures and the complex salts; therefore it is an important task to discover and quantify the species arising from HAuCl4, in order to understand their role in the gold assisted reactions. Mass spectrum of HAuCl4in a reflector negative-ion mode contains the hydrated mono- and dinuclear gold clusters in the m/z range 286–436, and gold chloride clusters in the m/z range 447–795. In the first part of spectrum, m/z range 286–436, the hydrated gold cluster species of type Aun−(H2O)m(n = 1–2; m = 1, 2, 5, 7, 8) and [Aun(OH)k]−(H2O)m(n = 1–2; k = 1–2; m = 1, 4–8) were found. Besides that, there are gold chloride clusters with general formula [AuHr(HCl)2]−(H2O)m(m = 1–5; 8–9; r = 0–2) in this part of spectrum. In the second part of spectrum, the m/z range 447–795, only gold chloride clusters were obtained. Their general formulae can be written as [AuClt(HCl)v]−(H2O)m(t = 1–4; v = 5–8; m = 2–4, 6–8) and [Aun(HCl)v]−(H2O)m(n = 1–2, v = 4–5, m = 1–2, 5, 7). The analysis of concentration effects on the LDI mass spectra of gold clusters reveals that the relative intensities of signals for the mono- and dinuclear Au clusters increase with decreasing the concentration of water HAuCl4solutions.
T1  - Gold chloride cluster ions generated by vacuum laser ablation
author = "Rajčić, Boris and Dimitrijević, Silvana B. and Petković, Marijana and Nišavić, Marija and Cindrić, Mario and Veljković, Filip M. and Veličković, Suzana",
abstract = "In this work, a simple way for study the possibility of formation a vapor cluster species of tetrachloroauric acid (HAuCl4), using the laser ablation in the absence of a buffer or reactive atmosphere, and without a postablation supersonic expansion on a commercial matrix assisted laser desorption/ionization time-of-flight mass spectrometer, is reported. Tetrachloroauric acid is known as precursor for the synthesis of gold nanostructures and the complex salts; therefore it is an important task to discover and quantify the species arising from HAuCl4, in order to understand their role in the gold assisted reactions. Mass spectrum of HAuCl4in a reflector negative-ion mode contains the hydrated mono- and dinuclear gold clusters in the m/z range 286–436, and gold chloride clusters in the m/z range 447–795. In the first part of spectrum, m/z range 286–436, the hydrated gold cluster species of type Aun−(H2O)m(n = 1–2; m = 1, 2, 5, 7, 8) and [Aun(OH)k]−(H2O)m(n = 1–2; k = 1–2; m = 1, 4–8) were found. Besides that, there are gold chloride clusters with general formula [AuHr(HCl)2]−(H2O)m(m = 1–5; 8–9; r = 0–2) in this part of spectrum. In the second part of spectrum, the m/z range 447–795, only gold chloride clusters were obtained. Their general formulae can be written as [AuClt(HCl)v]−(H2O)m(t = 1–4; v = 5–8; m = 2–4, 6–8) and [Aun(HCl)v]−(H2O)m(n = 1–2, v = 4–5, m = 1–2, 5, 7). The analysis of concentration effects on the LDI mass spectra of gold clusters reveals that the relative intensities of signals for the mono- and dinuclear Au clusters increase with decreasing the concentration of water HAuCl4solutions.",
title = "Gold chloride cluster ions generated by vacuum laser ablation",
Rajčić, B., Dimitrijević, S. B., Petković, M., Nišavić, M., Cindrić, M., Veljković, F. M.,& Veličković, S.. (2018). Gold chloride cluster ions generated by vacuum laser ablation. in Optical and Quantum Electronics, 50(5), 218.
Rajčić B, Dimitrijević SB, Petković M, Nišavić M, Cindrić M, Veljković FM, Veličković S. Gold chloride cluster ions generated by vacuum laser ablation. in Optical and Quantum Electronics. 2018;50(5):218.
Rajčić, Boris, Dimitrijević, Silvana B., Petković, Marijana, Nišavić, Marija, Cindrić, Mario, Veljković, Filip M., Veličković, Suzana, "Gold chloride cluster ions generated by vacuum laser ablation" in Optical and Quantum Electronics, 50, no. 5 (2018):218,
Structure analysis of geopolymers synthesized from clay originated from Serbia
Nenadović, Snežana S.; Kljajević, Ljiljana M.; Nešić, Maja A.; Petković, Marijana; Trivunac, Katarina V.; Pavlović, Vladimir B.
AU  - Trivunac, Katarina V.
AU  - Pavlović, Vladimir B.
AB  - The paper presents chemical and structural analysis of geopolymer materials which are obtained by alkali-activated calcined clay (metakaolin) originated from Serbia under strictly defined conditions. Characterization of the metakaolin and geopolymers molecular structure has been done using X-ray diffraction, scanning electron microscope, Fourier transform infrared spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The paper presents the possibility of obtaining geopolymer structure and differences in chemical and structural characterization of these materials taking into account the concentration of NaOH as a variable parameter. The results of MALDI analysis of metakaolin and synthesized geopolymer structures using various matrix system: 2,4,6 trihydroxyacetophenone (THAP), alpha-cyano-4-hydroxycinnamic, 2,6 dihydroxyacetophenone and laser desorption/ionization, have shown that THAP matrix is the most appropriate for analysing these aluminosilicate materials.
T2  - Environmental Earth Sciences
T1  - Structure analysis of geopolymers synthesized from clay originated from Serbia
author = "Nenadović, Snežana S. and Kljajević, Ljiljana M. and Nešić, Maja A. and Petković, Marijana and Trivunac, Katarina V. and Pavlović, Vladimir B.",
abstract = "The paper presents chemical and structural analysis of geopolymer materials which are obtained by alkali-activated calcined clay (metakaolin) originated from Serbia under strictly defined conditions. Characterization of the metakaolin and geopolymers molecular structure has been done using X-ray diffraction, scanning electron microscope, Fourier transform infrared spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The paper presents the possibility of obtaining geopolymer structure and differences in chemical and structural characterization of these materials taking into account the concentration of NaOH as a variable parameter. The results of MALDI analysis of metakaolin and synthesized geopolymer structures using various matrix system: 2,4,6 trihydroxyacetophenone (THAP), alpha-cyano-4-hydroxycinnamic, 2,6 dihydroxyacetophenone and laser desorption/ionization, have shown that THAP matrix is the most appropriate for analysing these aluminosilicate materials.",
journal = "Environmental Earth Sciences",
title = "Structure analysis of geopolymers synthesized from clay originated from Serbia",
Nenadović, S. S., Kljajević, L. M., Nešić, M. A., Petković, M., Trivunac, K. V.,& Pavlović, V. B.. (2017). Structure analysis of geopolymers synthesized from clay originated from Serbia. in Environmental Earth Sciences, 76(2).
Nenadović SS, Kljajević LM, Nešić MA, Petković M, Trivunac KV, Pavlović VB. Structure analysis of geopolymers synthesized from clay originated from Serbia. in Environmental Earth Sciences. 2017;76(2).
Nenadović, Snežana S., Kljajević, Ljiljana M., Nešić, Maja A., Petković, Marijana, Trivunac, Katarina V., Pavlović, Vladimir B., "Structure analysis of geopolymers synthesized from clay originated from Serbia" in Environmental Earth Sciences, 76, no. 2 (2017),
Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes
Rakić-Kostić, Tijana M.; Bogojeski, Jovana V.; Popović, Iva A.; Nešić, Maja D.; Rajčić, Boris; Nišavić, Marija; Petković, Marijana; Veličković, Suzana
AU  - Rakić-Kostić, Tijana M.
AU  - Bogojeski, Jovana V.
AB  - This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2 , 2 -terpyridine) palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). This complex is of interest for potential use in the cancer therapy. DoE methodology was proved to succeed in optimization of many complex analytical problems. However, it has been poorly used for MALDI-TOF-MS optimization up to now. The theoretical mathematical relationships which explain the influence of important experimental factors (laser energy, grid voltage and number of laser shots) on the selected responses (signal to noise - S/N ratio and the resolution - R of the leading peak) is established. The optimal instrumental settings providing maximal S/N and R are identified and experimentally verified.
T1  - Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes
author = "Rakić-Kostić, Tijana M. and Bogojeski, Jovana V. and Popović, Iva A. and Nešić, Maja D. and Rajčić, Boris and Nišavić, Marija and Petković, Marijana and Veličković, Suzana",
abstract = "This paper presents optimization of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer (MS) instrumental parameters for the analysis of chloro(2,2 , 2 -terpyridine) palladium(II) chloride dihydrate complex applying design of experiments methodology (DoE). This complex is of interest for potential use in the cancer therapy. DoE methodology was proved to succeed in optimization of many complex analytical problems. However, it has been poorly used for MALDI-TOF-MS optimization up to now. The theoretical mathematical relationships which explain the influence of important experimental factors (laser energy, grid voltage and number of laser shots) on the selected responses (signal to noise - S/N ratio and the resolution - R of the leading peak) is established. The optimal instrumental settings providing maximal S/N and R are identified and experimentally verified.",
title = "Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes",
doi = "10.2298/HEMIND160614038R"
Rakić-Kostić, T. M., Bogojeski, J. V., Popović, I. A., Nešić, M. D., Rajčić, B., Nišavić, M., Petković, M.,& Veličković, S.. (2017). Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes. in Hemijska industrija, 71(4), 281-288.
Rakić-Kostić TM, Bogojeski JV, Popović IA, Nešić MD, Rajčić B, Nišavić M, Petković M, Veličković S. Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes. in Hemijska industrija. 2017;71(4):281-288.
doi:10.2298/HEMIND160614038R .
Rakić-Kostić, Tijana M., Bogojeski, Jovana V., Popović, Iva A., Nešić, Maja D., Rajčić, Boris, Nišavić, Marija, Petković, Marijana, Veličković, Suzana, "Experimental design for optimizing MALDI-TOF-MS analysis of palladium complexes" in Hemijska industrija, 71, no. 4 (2017):281-288,
https://doi.org/10.2298/HEMIND160614038R . .
Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry
Kamceva, Tina T.; Nešić, Maja D.; Stoiljković, Milovan; Popović, Iva A.; Miletić, Jadranka; Rajčić, Boris; Petković, Marijana; Veličković, Suzana
AU  - Kamceva, Tina T.
AU  - Stoiljković, Milovan
AB  - In this work it has been shown that both the laser desorption/ionization mass spectrometry (LDI MS) and the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) are the simple and quick methods for determination of relative natural isotopic distribution of lead. The analysis of metal salts with these approaches does not require any time-consuming preparation of samples: a single run can take only a minute, and numerous information can be obtained. Results obtained in this work show that chosen matrix has no negative effect on quantitative determination of lead isotopes and support once more the applicability of MALDI TOF MS for lead isotope distribution determination in the sample and accurate data are obtained. Additionally, the generation of PbnOn and PbnOn-1 (n: 2-6) clusters have been successfully achieved in the positive mode, using the both LDI and MALDI methods. All stoichiometries were confirmed using isotopic pattern modelling.
T1  - Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry
DO  - 10.2298/HEMIND151218013K
author = "Kamceva, Tina T. and Nešić, Maja D. and Stoiljković, Milovan and Popović, Iva A. and Miletić, Jadranka and Rajčić, Boris and Petković, Marijana and Veličković, Suzana",
abstract = "In this work it has been shown that both the laser desorption/ionization mass spectrometry (LDI MS) and the matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) are the simple and quick methods for determination of relative natural isotopic distribution of lead. The analysis of metal salts with these approaches does not require any time-consuming preparation of samples: a single run can take only a minute, and numerous information can be obtained. Results obtained in this work show that chosen matrix has no negative effect on quantitative determination of lead isotopes and support once more the applicability of MALDI TOF MS for lead isotope distribution determination in the sample and accurate data are obtained. Additionally, the generation of PbnOn and PbnOn-1 (n: 2-6) clusters have been successfully achieved in the positive mode, using the both LDI and MALDI methods. All stoichiometries were confirmed using isotopic pattern modelling.",
title = "Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry",
doi = "10.2298/HEMIND151218013K"
Kamceva, T. T., Nešić, M. D., Stoiljković, M., Popović, I. A., Miletić, J., Rajčić, B., Petković, M.,& Veličković, S.. (2017). Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry. in Hemijska industrija, 71(1), 19-26.
https://doi.org/10.2298/HEMIND151218013K
Kamceva TT, Nešić MD, Stoiljković M, Popović IA, Miletić J, Rajčić B, Petković M, Veličković S. Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry. in Hemijska industrija. 2017;71(1):19-26.
doi:10.2298/HEMIND151218013K .
Kamceva, Tina T., Nešić, Maja D., Stoiljković, Milovan, Popović, Iva A., Miletić, Jadranka, Rajčić, Boris, Petković, Marijana, Veličković, Suzana, "Determination of isotopic distribution of lead by a matrix assisted laser desorption/ionization versus a laser desorption/ionization time of flight mass spectrometry" in Hemijska industrija, 71, no. 1 (2017):19-26,
https://doi.org/10.2298/HEMIND151218013K . .
Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
Nešić, Maja A.; Žakula, Jelena; Korićanac, Lela; Stepić, Milutin; Radoičić, Marija B.; Popović, Iva A.; Šaponjić, Zoran; Petković, Marijana
AB  - We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Journal of Photochemistry and Photobiology. A: Chemistry
T1  - Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
DO  - 10.1016/jjphotochem.2017.06.045
author = "Nešić, Maja A. and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Radoičić, Marija B. and Popović, Iva A. and Šaponjić, Zoran and Petković, Marijana",
abstract = "We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Journal of Photochemistry and Photobiology. A: Chemistry",
title = "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex",
doi = "10.1016/jjphotochem.2017.06.045"
Nešić, M. A., Žakula, J., Korićanac, L., Stepić, M., Radoičić, M. B., Popović, I. A., Šaponjić, Z.,& Petković, M.. (2017). Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry, 347, 55-66.
https://doi.org/10.1016/jjphotochem.2017.06.045
Nešić MA, Žakula J, Korićanac L, Stepić M, Radoičić MB, Popović IA, Šaponjić Z, Petković M. Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry. 2017;347:55-66.
doi:10.1016/jjphotochem.2017.06.045 .
Nešić, Maja A., Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Radoičić, Marija B., Popović, Iva A., Šaponjić, Zoran, Petković, Marijana, "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex" in Journal of Photochemistry and Photobiology. A: Chemistry, 347 (2017):55-66,
https://doi.org/10.1016/jjphotochem.2017.06.045 . .
The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains
Dimkic, Ivica; Stanković, Slavka; Nišavić, Marija; Petković, Marijana; Ristivojevic, Petar; Fira, Djordje; Beric, Tanja
AU  - Dimkic, Ivica
AU  - Stanković, Slavka
AU  - Ristivojevic, Petar
AU  - Fira, Djordje
AU  - Beric, Tanja
AB  - In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.
T2  - Frontiers in Microbiology
T1  - The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains
DO  - 10.3389/fmicb.2017.00925
author = "Dimkic, Ivica and Stanković, Slavka and Nišavić, Marija and Petković, Marijana and Ristivojevic, Petar and Fira, Djordje and Beric, Tanja",
abstract = "In this study the efficacy of two different methods for extracting lipopeptides produced by five Bacillus strains-ethyl acetate extraction, and acid precipitation followed by methanol extraction-was investigated using mass spectrometry. High performance thin layer chromatography (HPTLC) was also used for the simultaneous separation of complex mixtures of lipopeptide extracts and for the determination of antimicrobial activity of their components. The mass spectra clearly showed well-resolved groups of peaks corresponding to different lipopeptide families (kurstakins, iturins, surfactins, and fengycins). The ethyl acetate extracts produced the most favorable results. The extracts of SS-12.6, SS-13.1, and SS-38.4 showed the highest inhibition zones. An iturin analog is responsible for the inhibition of Xanthomonas arboricola and Pseudomonas syringae phytopathogenic strains. HPTLC bioautography effectively identified the active compounds from a mixture of lipopeptide extracts, proving in situ its potential for use in direct detection and determination of antimicrobials. In the test of potential synergism among individual extracts used in different mixtures, stronger antimicrobial effects were not observed. Biochemical and phylogenetic analysis clustered isolates SS-12.6, SS-13.1, SS-27.2, and SS-38.4 together with Bacillus amyloliquefaciens, while SS-10.7 was more closely related to Bacillus pumilus.",
journal = "Frontiers in Microbiology",
title = "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains",
doi = "10.3389/fmicb.2017.00925"
Dimkic, I., Stanković, S., Nišavić, M., Petković, M., Ristivojevic, P., Fira, D.,& Beric, T.. (2017). The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology, 8.
https://doi.org/10.3389/fmicb.2017.00925
Dimkic I, Stanković S, Nišavić M, Petković M, Ristivojevic P, Fira D, Beric T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00925 .
Dimkic, Ivica, Stanković, Slavka, Nišavić, Marija, Petković, Marijana, Ristivojevic, Petar, Fira, Djordje, Beric, Tanja, "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00925 . .
The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017)
TY  - GEN
T1  - The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017)
@misc{
title = "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017)",
Dimkic, I., Stanković, S., Nišavić, M., Petković, M., Ristivojevic, P., Fira, D.,& Beric, T.. (2017). The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017). in Frontiers in Microbiology, 8.
Dimkic I, Stanković S, Nišavić M, Petković M, Ristivojevic P, Fira D, Beric T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017). in Frontiers in Microbiology. 2017;8.
Dimkic, Ivica, Stanković, Slavka, Nišavić, Marija, Petković, Marijana, Ristivojevic, Petar, Fira, Djordje, Beric, Tanja, "The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains (vol 8, pg 925, 2017)" in Frontiers in Microbiology, 8 (2017),
Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin
Nišavić, Marija; Masnikosa, Romana; Butorac, Ana; Perica, Kristina; Rilak, Ana; Korićanac, Lela; Hozic, Amela; Petković, Marijana; Cindrić, Mario
AU  - Masnikosa, Romana
AU  - Butorac, Ana
AU  - Perica, Kristina
AU  - Rilak, Ana
AU  - Hozic, Amela
AB  - Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.
T1  - Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin
author = "Nišavić, Marija and Masnikosa, Romana and Butorac, Ana and Perica, Kristina and Rilak, Ana and Korićanac, Lela and Hozic, Amela and Petković, Marijana and Cindrić, Mario",
abstract = "Hyphenated mass spectrometry (MS) techniques have attained an important position in analysis of covalent and non-covalent interactions of metal complexes with peptides and proteins. The aim of the present study was to qualitatively and quantitatively determine ruthenium binding sites on a protein using tandem mass spectrometry and allied techniques, i.e. liquid chromatography (LC) and inductively coupled plasma optical emission spectrometry (ICP-OES). For that purpose, two newly synthesized Ru(II) complexes of a meridional geometry, namely mer-[Ru(4 Cl-tpy)(en)Cl](+) (1) and mer-[Ru(4 Cl-tpy)(dach)Cl](+) (2) (where 4 Cl-tpy = 4-chloro-2,2:6,2 -terpyridine, en = 1,2-diaminoethane and dach = 1,2-diaminocyclohexane), and bovine serum albumin were used. The binding of the complexes to the protein was investigated by means of size exclusion- and reversed phase-LC, ICP OES, matrix-assisted laser desorption ionization MS and MS/MS. Ruthenated peptide sequence and a binding target amino acid were revealed through accurate elucidation of MS/MS spectra. The results obtained in this study suggest a high binding capacity of the protein towards both complexes, with up to 5.77 +/- 0.14 and 6.95 +/- 0.43 mol of 1 and 2 bound per mol of protein, respectively. The proposed binding mechanism for the selected complexes includes the release of Cl ligand, its replacement with water molecule and further coordination to electron donor histidine residue. (C) 2016 Elsevier Inc. All rights reserved.",
title = "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin",
Nišavić, M., Masnikosa, R., Butorac, A., Perica, K., Rilak, A., Korićanac, L., Hozic, A., Petković, M.,& Cindrić, M.. (2016). Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry, 159, 89-95.
Nišavić M, Masnikosa R, Butorac A, Perica K, Rilak A, Korićanac L, Hozic A, Petković M, Cindrić M. Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin. in Journal of Inorganic Biochemistry. 2016;159:89-95.
Nišavić, Marija, Masnikosa, Romana, Butorac, Ana, Perica, Kristina, Rilak, Ana, Korićanac, Lela, Hozic, Amela, Petković, Marijana, Cindrić, Mario, "Elucidation of the binding sites of two novel Ru(II) complexes on bovine serum albumin" in Journal of Inorganic Biochemistry, 159 (2016):89-95,
SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates
Popović, Iva A.; Nešić, Maja A.; Vranješ, Mila; Šaponjić, Zoran; Petković, Marijana
AB  - Surface-assisted laser desorption/ionisation time-of-flight mass spectrometry (SALDI-TOF-MS) might be the method of choice for the analysis of low mass molecules (less than m/z 500). Titanium dioxide (TiO2) nanocrystals as a substrate for SALDI-TOF-MS improve the reproducibility of the signal intensities and prevent the fragmentation of some molecules upon laser irradiation, as we have previously shown. In addition, variously shaped and sized TiO2 nanocrystals/substrates for SALDI-MS could be used for quantification of small molecules, which are otherwise difficult to detect with the assistance of organic matrices. TiO2-assisted LDI-MS spectra could be acquired with excellent reproducibility and repeatability and with low detection limit. In the current study, we analysed the spectra of dexasone, citric acid, vitamin E and vitamin A acquired with TiO2 nanocrystals of various shapes and dimensions, i.e. the colloidal TiO2 nanoparticles (TiO2 NPs), TiO2 prolate nanospheroids (TiO2 PNSs) and TiO2 nanotubes (TiO2 NTs). Various shapes and dimensions of substrates were used since these factors determine desorption and ionisation processes. The homogeneity on the target plate was compared based on signal-to-noise values of peaks of interest of analysed molecules as well as the within-day and day-to-day repeatability. In summary, the obtained results show that the applicability of individual TiO2 nanocrystals depends on the analyte. Signals which are acquired with the assistance of TiO2 PNSs have the highest sensitivity and reproducibility (the smallest standard deviation), even compared with those in the LDI mode. This implies that TiO2 PNSs could also be suitable for quantitative analyses of small molecules.
T2  - Analytical and Bioanalytical Chemistry
T1  - SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates
author = "Popović, Iva A. and Nešić, Maja A. and Vranješ, Mila and Šaponjić, Zoran and Petković, Marijana",
abstract = "Surface-assisted laser desorption/ionisation time-of-flight mass spectrometry (SALDI-TOF-MS) might be the method of choice for the analysis of low mass molecules (less than m/z 500). Titanium dioxide (TiO2) nanocrystals as a substrate for SALDI-TOF-MS improve the reproducibility of the signal intensities and prevent the fragmentation of some molecules upon laser irradiation, as we have previously shown. In addition, variously shaped and sized TiO2 nanocrystals/substrates for SALDI-MS could be used for quantification of small molecules, which are otherwise difficult to detect with the assistance of organic matrices. TiO2-assisted LDI-MS spectra could be acquired with excellent reproducibility and repeatability and with low detection limit. In the current study, we analysed the spectra of dexasone, citric acid, vitamin E and vitamin A acquired with TiO2 nanocrystals of various shapes and dimensions, i.e. the colloidal TiO2 nanoparticles (TiO2 NPs), TiO2 prolate nanospheroids (TiO2 PNSs) and TiO2 nanotubes (TiO2 NTs). Various shapes and dimensions of substrates were used since these factors determine desorption and ionisation processes. The homogeneity on the target plate was compared based on signal-to-noise values of peaks of interest of analysed molecules as well as the within-day and day-to-day repeatability. In summary, the obtained results show that the applicability of individual TiO2 nanocrystals depends on the analyte. Signals which are acquired with the assistance of TiO2 PNSs have the highest sensitivity and reproducibility (the smallest standard deviation), even compared with those in the LDI mode. This implies that TiO2 PNSs could also be suitable for quantitative analyses of small molecules.",
journal = "Analytical and Bioanalytical Chemistry",
title = "SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates",
Popović, I. A., Nešić, M. A., Vranješ, M., Šaponjić, Z.,& Petković, M.. (2016). SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates. in Analytical and Bioanalytical Chemistry, 408(26), 7481-7490.
Popović IA, Nešić MA, Vranješ M, Šaponjić Z, Petković M. SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates. in Analytical and Bioanalytical Chemistry. 2016;408(26):7481-7490.
Popović, Iva A., Nešić, Maja A., Vranješ, Mila, Šaponjić, Zoran, Petković, Marijana, "SALDI-TOF-MS analyses of small molecules (citric acid, dexasone, vitamins E and A) using TiO2 nanocrystals as substrates" in Analytical and Bioanalytical Chemistry, 408, no. 26 (2016):7481-7490,