###Human: When does Methotrexate lower hCG level in ectopic pregnancy? ###Assistant: Hello. I hope you had taken the Methotrexate dose under supervision only. The usual protocol is to check for a repeat beta hCG on the fourth day of taking Methotrexate. Early testing may cause undue anxiety and panic as it takes some time for the levels to come down. Moreover, a few times the levels get slightly elevated after a Methotrexate. So stay calm and follow the instructions of the gynecologist you are following. Just report to the emergency in case of any acute pain, giddiness or bleeding.