TOX21_PPARd_BLA_Antagonist_ch1 is an assay readout measuring reporter gene via receptor activity and designed using inducible reporter (beta lactamase induction) detected with GAL4 b-lactamase reporter gene. The signal is derived from the uncleaved reporter gene substrate and is used to calculate the ratio of cleaved (ch2) to uncleaved (ch1) substrate used as the measure of target activity. Data from the assay component TOX21_PPARd_BLA_Antagonist_ch1 was analyzed into 1 assay endpoint. This assay endpoint, TOX21_PPARd_BLA_Antagonist_ch1, was analyzed in the positive fitting direction relative to DMSO as the negative control and baseline of activity. Using a type of inducible reporter,increased activity can be used to understand changes in the reporter gene as they relate to the gene PPARD. Furthermore, this assay endpoint can be referred to as a secondary readout, because this assay has produced multiple assay endpoints where this one serves as artifact detection function. Is this molecule effective to this assay?