TOX21_MMP_ratio, is one of 2 assay component(s) measured or calculated from the TOX21_MMP assay. It is designed to make measurements of dye binding, a form of membrane potential reporter, as detected with fluorescence intensity signals by homogenous mitochondrial membrane potential assay technology.Data from the assay component TOX21_MMP_ratio was analyzed into 2 assay endpoints. The assay endpoin, TOX21_MMP_ratio_up, was analyzed in the positive fitting direction relative to DMSO as the negative control and baseline of activity. Using a type of membrane potential reporter, gain-of-signal activity can be used to understand changes in the signaling. Furthermore, this assay endpoint can be referred to as a primary readout, because this assay has produced multiple assay endpoints where this one serves a signaling function. To generalize the intended target to other relatable targets, this assay endpoint is annotated to the cell morphology intended target family, where the subfamily is organelle conformation. Is this molecule effective to this assay?