The assay involves a high throughput screening of small molecules that potentially reduce or block internalization of anthrax toxin. LF-beta-lactamase fusion proteins were used to generate a quantitative assay that measures beta-lactamase activity in cells in the presence of PA. The assay relies on the FRET system involving a fluorescent substrate (CCF2/AM) trapped in cells after cleavage by cytoplasm esterases. The EnVision plate reader was used to monitor fluorescence intensity. The data were plotted as a ratio of the 460 nm/530 nm emissions. Is the molecule effective to this assay?