The assay is focused on developing potent and validated inhibitors for orthopoxviruses, including smallpox and monkeypox, which have no FDA-licensed drugs to treat infected individuals. The assay uses a quantitative high throughput method using a vaccinia virus model system to identify novel anti-orthopoxviral compounds. The assay uses a coupled reporter system that monitors viral gene expression and viral spread. The goal is to find compounds that inhibit viral gene expression and prevent viral spread. Is the molecule effective to this assay?