The article discusses how the immune system fights infections by activating antigen specific T cells, which undergo a proliferative expansion and differentiation process to generate potent pathogen-specific effector T cells. Immunological tolerance prevents T cells from attacking self peptides, and negative feedback mechanisms serve to tune down TCR signaling in response to chronic antigens. Chronic viral, bacterial, and parasitic infections can evade a robust immune response by allowing T cells to tune down their signaling, and disrupting the tuning could allow T cells to function again and deliver a robust effector response. A sensitive HTS T cell based assay based on IL-2 is used to find small molecules in the Molecular Libraries Small Molecule Repository (MLSMR) that could elicit a T cell response. Is the molecule effective to this assay?