The project aimed to develop a new assay to discover small molecule ligands for class B1 G protein-coupled receptors, focusing specifically on the GLP-1R receptor. The assay used stably transfected HEK293 cells that co-expressed a cAMP-responsive luciferase reporter gene and constitutively active GLP-1R. The goal was to find inverse agonists that would inhibit luciferase activity. The assay was optimized for screening in a 1536-well format and was screened against the MLSMR. Is the molecule effective to this assay?