The assay aims to identify compounds that induce DNA re-replication in normal cells. The assay utilizes the fluorescent DNA binding dye, Hoechst, to measure the DNA content of nuclei. Cell nuclei are imaged, and compounds that trigger re-replication are identified based on an increase in nuclei displaying elevated fluorescence intensity. Key regulators of the process include cell cycle components such as cyclin A, Cdt-1, Cdc6, and geminin. Misregulation of these components can lead to DNA re-replication in both normal and cancer cells. Is the molecule effective to this assay?