The antioxidant response element (ARE) is a transcriptional regulatory element involved in the activation of genes coding for a number of antioxidant proteins and detoxifying enzymes. The protective effects of ARE activation are primarily triggered through Nrf2 binding to and activating AREs. Nrf2 controls the production of over 250 antioxidant and detoxification proteins, thereby protecting tissues by increasing cellular antioxidant content and suppressing inflammatory signaling pathways. Identifying novel and potent Nrf2 activators by using the AREc32 cell line to detect ARE activation by small molecules will lead to the development of probes to study protective pathways in multiple tissues for the treatment of cardiovascular diseases, obesity, diabetes, Alzheimer's, and Parkinson's disease. Is the molecule effective to this assay?