The assay focuses on the transcription factor Nrf2, which protects cells from harmful substances and maintains cellular redox balance. Nrf2 activates genes that encode for antioxidants, providing a first line of defense against stress caused by radiation, electrophiles, and xenobiotics. Tumor cells often manipulate the Nrf2 pathway to resist chemotherapy and radiotherapy, making Nrf2 inhibition a potential therapeutic target to improve patient survival. A small molecule inhibitor of Nrf2 function could represent a novel treatment option. Is the molecule effective to this assay?