The assay aims to identify small molecule inhibitors that target the JMJD2A-tudor domain interaction with its target methylhistone mark using a quantitative high throughput AlphaScreen. Methylation is a post-transcriptional modification that affects epigenetic transcription, and JmjD2A is a lysine demethylase that specifically binds to trimethyl H3K4 and H4K20 marks. Small molecule targeting of this interaction is expected to enable sophisticated experiments probing the fine regulatory pathways leading to selective demethylation of a given methyllysine locus based on the methylation state of adjacent histone marks. However, caution should be taken due to potential screening artifacts, and an AlphaScreen counterscreen is recommended to eliminate non-specific hits. Is the molecule effective to this assay?