This assay uses a chemiluminescence based AlphaScreen to identify inhibitors of G9a, a histone lysine methyltransferase involved in the epigenetic regulation of gene expression. Inhibition of G9a may be a potential drug target for diseases such as cancer. The assay measures the methylation of biotinylated histone peptide through specific antibody-based detection, and is well-suited for detection of inhibitors acting by the desired histone peptide competitive mechanism. However, caution is advised when interpreting primary screening data due to the prevalence of screening artifacts, and a counterscreen is recommended to eliminate non-specific hits. Is the molecule effective to this assay?