This validation qHTS assay focuses on human BLM, which is important for resolving abnormal DNA structures. The activity was measured as ATP-dependent separation of a forked DNA substrate tagged with a rhodamine fluorophore and BHQ-2 dark quencher. Interference from compounds such as DNA intercalators was reduced by including poly (dIdC) in the assay. The assay aims to identify drugs that interfere with DNA repair, sensitizing cancer cells to conventional therapy. Is the molecule effective to this assay?