This assay monitors the potentiation of the binding of a Cy5-labeled SRC-1 peptide to the ligand binding domain (LBD) of ER-alpha labeled (via a streptavidin-biotin interaction) with a long-lifetime europium. The interaction of ER-alpha with SRC1 under a low estradiol concentration brings europium and Cy5 into proximity, generating basal level of FRET. Compounds that can further enhance the interaction with increased FRET signal are identified as positives for further analysis. These compounds could either be conventional. Is this molecule potentiator to ER-alpha? This assay monitors the potentiation of the binding of a Cy5-labeled SRC-1 peptide to the ligand binding domain (LBD) of ER-alpha labeled (via a streptavidin-biotin interaction) with a long-lifetime europium. The interaction of ER-alpha with SRC1 under a low estradiol concentration brings europium and Cy5 into proximity, generating basal level of FRET. Is this molecule potentiator to ER-alpha? This assay monitors the potentiation of the binding of a Cy5-labeled SRC-1 peptide to the ligand binding domain (LBD) of ER-alpha labeled (via a streptavidin-biotin interaction) with a long-lifetime europium. The interaction of ER-alpha with SRC1 under a low estradiol concentration brings europium and Cy5 into proximity, generating basal level of FRET. Is this molecule effective to ER-alpha? This assay monitors the potentiation of the binding of a Cy5-labeled SRC-1 peptide to the ligand binding domain (LBD) of ER-alpha labeled (via a streptavidin-biotin interaction) with a long-lifetime europium. The interaction of ER-alpha with SRC1 under a low estradiol concentration brings europium and Cy5 into proximity, generating basal level of FRET. Is this molecule effective to this assay?