The PubChem BioAssay is a luminescence-based biochemical assay which is primarily used to determine the dose response of inhibitors that can act on Trypanosoma brucei methionyl tRNA synthetase (MetRS). The assay classifies as a confirmatory assay and it is categorized as a direct single protein target assigned. The assay is specifically designed for Trypanosoma brucei brucei strain 927/4 GUTat10.1 and its assay type description is functional. Hence, this assay is highly useful to confirm the effectiveness of potential inhibitors of MetRS in treating the diseases caused by Trypanosoma brucei. Does this assay show efficacy of the molecule?