text: Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16"
  
  Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
  <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
  
  Objective
  
  We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol
  
  Description
  
  1. Add experimental record here. Include what, how, and why...
  
  Data
  
  Stock Solution
  
  1. Buffer
  1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
  1. Dissolve 12.9mg luminol in 300uL of DMSO
  2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
  1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
  2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM
  
  Notes
  
  - We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300
  
  Graph showing the absorbance spectra for citrate AuNP and HRP AuNP
  
  Making the BSA AuNP solution Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA
  
  ```   - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA
  [BSA] = 15.6μM
  - (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution
  ```
  
  Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours
explanation: The document appears to be a lab notebook entry detailing an experiment involving the testing of HRP-NPs (Horseradish Peroxidase Nanoparticles) for the catalytic conversion of luminol. It includes a description of the experiment, data on stock solutions, and notes on absorbance measurements. There are also calculations for preparing a BSA AuNP solution. The document does not contain any explicit math questions or answers.
distilabel_metadata.content: Evaluate the user given text extract and determine if it contains a math question and optionally and answer to this question.
  
  Output rules:
  
  1. Briefly describe the document and mention if it contains a question or a question and answer.
  2. Finalize the output with: Label: no-question,question,question-and-answer,multiple-question,multiple-question-and-answer
distilabel_metadata.role: system
distilabel_metadata.content: Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16"
  
  Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
  <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
  
  Objective
  
  We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol
  
  Description
  
  1. Add experimental record here. Include what, how, and why...
  
  Data
  
  Stock Solution
  
  1. Buffer
  1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
  2. Luminol
  1. Dissolve 12.9mg luminol in 300uL of DMSO
  2. Add to 50mL buffer ---> 1.46mM
  3. H2O2
  1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
  2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM
  
  Notes
  
  - We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300
  
  Graph showing the absorbance spectra for citrate AuNP and HRP AuNP
  
  Making the BSA AuNP solution Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA
  
  ```   - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA
  [BSA] = 15.6μM
  - (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution
  ```
  
  Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours
distilabel_metadata.role: user
distilabel_metadata.raw_output_q_a_detector_0: The document appears to be a lab notebook entry detailing an experiment involving the testing of HRP-NPs (Horseradish Peroxidase Nanoparticles) for the catalytic conversion of luminol. It includes a description of the experiment, data on stock solutions, and notes on absorbance measurements. There are also calculations for preparing a BSA AuNP solution. The document does not contain any explicit math questions or answers.
  
  Label: no-question