The Abenaki First Nation of Odanak studied lake sturgeon (Acipenser fulvescens) reproduction in the Saint-François River (Québec, Canada), just downstream of the Drummondville hydroelectric plant, from 2012 to 2018. The spawning population was assessed, egg deposition sites were located, and larval production and reproductive success were estimated. This study aimed to determine if interannual variability in the abundance of spawners and larvae were influenced by water flow rate and temperature. Between 54 and 188 spawners used 3 separate sites for egg deposition. The number of larvae produced differed between years, ranging from 8,953 to 44,218, representing a reproductive success ranging from 0.29% to 4.59%. The results suggest that the river flow rate during migration positively influenced annual spawner abundance. However, water temperature did not affect the number of spawners or larvae. The highest larval abundance values were observed under a stable flow rate of 85 m3/s during larval drift. Thanks to the work conducted by the Abenaki community of Odanak, it has been possible to inform and guide fishery and water flow management to support and enhance lake sturgeon conservation.