The Staying Connected Initiative (SCI) is a binational collaboration between more than 55 public and private entities in Canada and the United States, including public transportation and wildlife agencies, universities and conservation organizations. The SCI partners have been working together since 2009 to sustain landscape connectivity across the Northern Appalachian/Acadian ecoregion, and they have implemented a suite of strategies to make roads safer for wildlife and people. Partners collaborate in spatial analyses to identify road segments that are key to maintaining connectivity; participate in the validation of critical wildlife passages; and are involved in choosing designs to facilitate safe passage for wildlife and to reduce roadkill. To ensure that wildlife can access habitats beyond the right-of-way, the SCI partners use measures such as land protection along wildlife corridors (including along roadsides), land-use planning, and public engagement, and they provide support for policy to safeguard investments made for landscape connectivity.