Compared to the rest of Quebec (Canada), the St. Lawrence Lowlands (SLL) exhibit a particularly high biological diversity, and are home to many species at risk. This part of the province is also where anthropogenic activities that affect ecosystem integrity are concentrated. An atlas of sites of conservation interest within the SLL has recently been produced to identify locations where conservation actions are required (Jobin et al., 2019a). The conservation targets (coarse filter) are woodlands, wetlands, open habitats (e.g., old fields and perennial crops) and aquatic environments. Sites of interest were determined by first selecting those of very high conservation value (e.g., presence of species at risk and protected areas), followed by a multi-criteria prioritization analysis. Local sites of importance for biodiversity (fine filter) are also provided, such as fish spawning grounds and bird colonies. This article presents an overview of the results of the atlas, possible applications of the results and potential uses for stakeholders involved in the conservation of natural areas in Quebec. As geospatial data are available for the sites of interest, users can identify their location and adapt the information presented to their particular area.