Elimination of phenol by two aquatic plants : Juncus fontanesii (Gay) and Lemna minor L.
Phenols are considered as toxic organic compounds. They can be treated by different physico-chemical or biological processes. These products can be oxidized by chemicals such as H2O2, TiO2, O3, etc. The performance of the process depends on pH, temperature and phenol/oxidant ratio. Otherwise, they can be transformed biologically by enzymes, fungi, yeast or plants. Considerable work has already been done with regard to uptake of phenol by aquatic plants. In our study two aquatic plants: Juncus fontanesii, a rooted species from Joncaceae family and Lemna minor, floating species from Lemnaceae family, have been selected to study their ability to remove phenol from static phenolic solutions. The initial concentration of phenol varied from 8 to 48 mg/l. The density of biomass (wet weight) ranged from 2.8 to 5.6 kg/m2. Experiences were carried out without acclimation and without addition of nutritive elements. Controls (without plants) were prepared with the same concentrations. Under these conditions, the results of quantitative analyses show that J. fontanesii is able to remove phenol more rapidly than L. minor and can release a fraction of it to the medium particularly in the first ten hours of contact. It has been observed that phenol uptake is sensitive to the density of biomass and the initial concentration. In order to examine more closely the effect of these variables, we have carried out experiments where the initial concentration was kept constant (8 mg/l) and biomass density varied. When the density of biomass increases, the kinetic uptake of phenol by J. fontanesii increases too; however, it decreases in the presence of L. minor. In fact, at high densities, L. minor covers fully the surface of the water and causes a screen effect, such that diffusion of atmospheric oxygen into the medium is limited. In addition, L. minor has a short root system, so the amount of oxygen that enters the solution is negligible. Elimination of phenol by L. minor is rapid when the density of biomass ranges from 0.7 to 1.4 kg/m2. For both plants, we have noticed the existence of a maximum time limit of degradation and an optimal density beyond which there is no improvement in elimination. Phenol can be degraded by micro-organisms. In order to elucidate this pathway, an investigation was undertaken using activated sludges in the following situations: under atmospheric conditions, under anaerobic conditions and with bubbling air intermittently. The comparison of obtained results shows that the rate and kinetics of the elimination decrease in the following order: J. fontanesii > L. minor (low densities) > micro-organisms with air bubbling > micro-organisms under atmospheric conditions > controls (without plants) > L. minor (high densities) > micro-organisms under anaerobic conditions.