Turtle population dynamics rely on the longevity of adults to compensate for low juvenile recruitment. Consequently, they cannot recover rapidly from the loss of individuals caused by excess mortality, such as roadkill, which exposes them to significant population declines—and can potentially lead to local extinction. In 2012, a study was initiated to document turtle roadkill rates along Route 245 (a regional road in the Estrie region of Québec, Canada), identified as a threat to turtle populations in the adjacent Missisquoi Nord River. Two years of data collection were sufficient to identify some of the problem areas. In parallel with the study, a partnership with local and regional stakeholders (including the Ministère des Forêts, de la Faune et des Parcs, the Ministère des Transports du Québec [MTQ] and the municipality of Bolton-Est) was initiated to implement roadkill mitigation measures in the most severely impacted areas. To facilitate the safe passage of turtles under Route 245, the MTQ integrated several of these measures into its scheduled culvert replacement work. All partners played a key role in the successes achieved and will continue to be involved during the ongoing monitoring phase.