Mechanisms of nitrogen and phosphorus removal in a high rate algal pond
The fate of nitrogen (N) and phosphorus (P) was studied in a high-rate algal pond (HRAP) during the start-up period and under steady-state conditions. The HRAP was first used by Oswald and co-workers in Berkeley (California, USA) in the late 1950s for wastewater treatment with algae collection as a by-product for Single Cell Protein production. The HRAP consisted of a raceway shaped, shallow pond (less than 0.50 m deep) equipped with a continuous and gentle mixing device (paddle wheel) generally operated at 8 rpm. The paddle wheel is not used for aeration purposes. In this study, the HRAP is included in a wastewater treatment plant aimed at producing good quality effluent for agriculture reuse purposes. The treatment plant includes three components with the HRAP placed in the middle of the pond train. Up-stream, there is a two-phase anaerobic reactor (TAR) and downstream, two maturation ponds in series. Assigned removal tasks for the plant components are as follows: i) organic matter, suspended solids and helminth eggs for the TAR; ii) N and P for the HRAP; and iii) fecal coliforms for the two maturation ponds. The capacity of the plant is 1,500 inhabitants (70 m3/day), total hydraulic retention time is 8 days and the total occupied area is 1,500 m2 including walkways and other facilities. The plant was constructed in December 1996 and has been continuously operated since that time. The present paper focuses on the fate of N and P in the HRAP in order to evaluate its performance and to shed light on the mechanisms behind N and P removal, with the ultimate goal to improve surface and groundwater protection from pollution by wastewater nutrients. Under steady-state conditions and when we consider the soluble part of N and P taken up by the algal cells together with that lost (either by stripping or chemical precipitation), the total removal averaged 70% for N and 40% for P. Such important removal must be highlighted especially because the system relies on solar energy with no electromechanical aeration, is cheap to construct and easy to operate and maintain. To understand the mechanisms behind such a performance and also to gain experience for the process start-up and for rational operation and maintenance of future plants, an in-depth analysis on the fate of N and P was done based on the four-year follow up data. Total N losses reached an average of 34.5% in the start-up period and 24.5% under steady-state conditions, whereas N assimilated by the algae represented 32 and 43.5%, respectively. Particulate organic nitrogen (PON) accumulated in the bottom of the unit did undergo rapid mineralization under steady-state conditions. Mechanisms involved in N losses might be attributed to settling of PONr and, to much lesser extent, NH3 volatilization in the cold season, whereas the latter mechanism might occur primarily in the hot season. The rates of mineralization of HRAP sediment were estimated to be 12.4 and 1.3 kg of N ha-1 d-1 respectively for the hot and the cold season. On average, total P losses in the HRAP reach 27% in the start-up period and only 17.5 % under steady-state conditions, whereas algae assimilation represented 25 and 17.5% respectively. Under steady-state conditions, algae uptake (54%) and chemical precipitation (46%) were found to be the main mechanisms for P removal in the HRAP.