Context: The study aims to assess knowledge of and attitudes towards HIV/AIDS among a sample of 658 university students in Northern Québec, Canada. Method: Cross-sectional study using a French-language translation of the Brief HIV Knowledge Questionnaire to assess students’ knowledge. Results: Overall, the students’ level of knowledge proved high, but the study also identifies marked gaps. Factors such as being born outside Canada (OR: 3.8 ; CI95%: 1.4-10.1), studying at the undergraduate level (as opposed to graduate ; OR: 2.5 ; CI95%: 1.2-5.1) and never having known anyone living with HIV/AIDS (OR: 2.1 ; CI95%: 1.2-3.8) were related to low levels of knowledge about HIV/AIDS. Conclusion: The results point to a need for improved awareness and education strategies among certain sub-groups of the university population.