Highway 10 in southern Québec (Canada) fragments natural habitats in the northern portion of the Green Mountains, which form part of the Appalachian chain. The objective of Appalachian Corridor and its partners is to maintain or restore habitat connectivity on both sides of this element of fragmentation. The multipronged approach chosen to achieve this first involves identifying the natural corridors on both sides of Highway 10, and the passages that need to be improved to increase wildlife permeability. The protocol, data collection and initial analyses were developed and conducted in partnership with research and conservation stakeholders, as well as with representatives from the relevant provincial ministries. The approach also involves crucial consultations with municipal authorities and local conservation groups to maintain or restore ecological connectivity through adequate protection of the natural environment within the identified corridors. This multidisciplinary approach, coupled with the development of strong partnerships, are essential for achieving solid results that improve road safety and wildlife permeability along Highway 10, and that protect the ecological connectivity of the area for biodiversity and facilitate its adaptation to climate change.