There are numerous instruments in the literature to measure motivation in physical activity and sport achieved in physical education context. However, tools for assessing motivation in physical and health education (PHE) courses are rare. To promote physical activity, PHE teachers are developing various teaching and assessment strategies. In this context, an instrument that measures motivation in PHE would help to assess the effectiveness of the strategies put in place. This study reports on the process of developing and validating such an instrument with French-speaking postsecondary students, including a pre-intervention version and a post-intervention version. Results indicate good psychometric properties for the instrument. These results are discussed in the light of the instrument’s foresights and the needs that justified its conduct.