various studies have shown that internal carotid artery ( ica ) atherosclerotic disease is a strong predictor of ischemic stroke [ 1 , 2 ] . the majority of these studies focused on the extracranial part of the ica and the association with intracranial atherosclerotic disease is generally not considered . however , kappelle et al .  
have demonstrated that the presence of intracranial ica atherosclerotic disease is an independent risk factor for subsequent stroke in patients with symptomatic extracranial ica stenosis .
furthermore , it has been estimated that intracranial arterial disease is responsible for approximately 510% or 3366% of ischemic strokes in the usa and asia , respectively [ 48 ] .
because of its tortuosity , the intracranial carotid siphon is a predilection site for early atherosclerotic lesions .
therefore , the presence of atherosclerotic disease in this segment of the ica may be an important prognostic factor in the prediction of recurrent stroke .
furthermore , reliable assessment of intracranial ica atherosclerotic disease may influence treatment approach of patients with acute ischemic stroke referred for endovascular treatment . in patients with severe carotid siphon stenosis , thrombectomy with mechanical devices
will be more difficult and hazardous than in those without . to definitely establish the significance of carotid siphon disease in future studies , there is an urgent need for accurate and reproducible measurement methods .
traditionally , digital subtraction angiography ( dsa ) is considered as the gold standard for evaluating intracranial carotid artery stenosis .
presently , dsa is no longer routinely performed in the diagnostic workup of stroke patients , and it is unethical even to apply it for research purposes only because of a small but non - negligible risk of serious complications .
nowadays , noninvasive or minimally invasive tests , such as ct angiography ( cta ) , are increasingly used in the evaluation of the carotid artery .
the diagnostic accuracy of cta in evaluating intracranial atherosclerotic stenosis compared with dsa has been assessed in previous studies [ 1013 ] .
these studies have shown high sensitivity and negative predictive value for detecting intracranial arterial stenosis in cta as compared with dsa .
this makes cta a potentially useful and accurate noninvasive diagnostic test for detecting intracranial stenosis .
however , to our knowledge , the interobserver agreement of the stenosis measurements of the ( often severely calcified ) intracranial ica in cta has not been addressed before .
for example , the delineation of the intracranial ica with cta causes difficulties because calcifications in the intracranial ica are in the same intensity range as the skull .
furthermore , there is overlap of intensity of the calcifications and contrast - enhanced lumen . in summary , up to now , there is a lack of studies on the quantitative measurement of the intracranial ica disease in cta images .
the aim of this study was to present a novel semi - automatic application to quantitatively measure the degree of intracranial ica stenosis and the calcium volume burden in the intracranial ica and to assess its interobserver agreement in a population of symptomatic patients .
in our center , in the routine diagnostic workup , symptomatic patients suspected of having carotid artery stenosis are primarily evaluated by duplex sonography .
all consecutive patients who underwent cta on a 64-section ct scanner for carotid stenosis evaluation between april 1 , 2006 , and december 31 , 2008 , were included in the present study .
patients with a previous carotid intervention were excluded . within a case of an occlusion of the extracranial part of the ica and of an unreliable reference diameter of the intracranial ica ,
permission of the medical ethics committee was given for this retrospective analysis of anonymized patient data .
no informed consent was required because the evaluation of the images was performed outside the clinical setting .
cta was performed with a 64-slice scanner ( brilliance 64 , philips healthcare , best , the netherlands ) .
an 18-gauge intravenous catheter was placed in the antecubital vein , and 80  ml of contrast ( visipaque 320 , ge healthcare ) was infused at 4  ml / s after an initial injection delay depending on an attenuation of 150  hu in the aortic arch .
acquisition and reconstruction parameters were as follows : tube voltage , 120  kv ; effective mas , 265 ; pitch , 0.765 ; increment , 0.45  mm ; slice thickness , 0.9 or 1.5  mm .
the scan ranged from the aortic arch up to 3  cm above the sella turcica .
aspirin symptomatic intracranial disease ( wasid ) method for determining the degree of stenosis in the intracranial ica
. the wasid degree of stenosis is calculated as\documentclass[12pt]{minimal } 
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				 \begin{document}$$ \left ( { 1 - \frac{{smallest\,diameter}}{{reference\,diameter } } } \right ) \times 100\% $ $ \end{document } the reference diameter is measured at the widest straight part of the petrous segment of the ica .
if the entire petrous part of the ica is diseased , the most distal parallel part of the extracranial ica is used as reference . in the cta images ,
a central lumen line was created by a single trained observer lb using the 3mensio vascular software ( bilthoven , the netherlands ) .
the centerline started at the vertical running part of the petrous segment of the ica closely adjacent to the skull base , which lies in the carotid canal , up to the middle cerebral artery  anterior cerebral artery bifurcation ( fig . 
1views of the semi - automatic software . a volume rendering view of the ct data including the central lumen line starting at the vertical petrous segment adjacent to the skull base up to the middle cerebral artery  anterior cerebral artery bifurcation .
b a view perpendicular to the central lumen line used for diameter measurements ; the circle could be adjusted to measure the lumen diameter .
c stretched vessel view providing an overview of the whole running of the vessel , which includes lumen diameters as estimated by the software .
d illustration of the adjustment of the region of interest of the calcium volume measurement , which can be performed with the circular contour editor .
d calculated calcium voxels , which are the voxels with an intensity above a threshold value ( of 420  hu ) within the region of interest views of the semi - automatic software . a volume rendering view of the ct data including the central lumen line starting at the vertical petrous segment adjacent to the skull base up to the middle cerebral artery 
b a view perpendicular to the central lumen line used for diameter measurements ; the circle could be adjusted to measure the lumen diameter . c stretched vessel view providing an overview of the whole running of the vessel , which includes lumen diameters as estimated by the software .
d illustration of the adjustment of the region of interest of the calcium volume measurement , which can be performed with the circular contour editor .
d calculated calcium voxels , which are the voxels with an intensity above a threshold value ( of 420  hu ) within the region of interest the centerline was used to generate additional views of the intracranial ica for the vessel analysis : a stretched vessel view , producing an overview of the whole running of the artery , and a perpendicular view , generating a view of the artery orthogonal to the centerline ( fig . 
subsequently , the observers independently measured the degree of stenosis , blinded for clinical information . to determine the degree of stenosis , the smallest diameter of intracranial ica up to the vertical running of segment c7 in the internal carotid artery classification of bouthillier   and a reference diameter of the intracranial ica was manually measured on the view perpendicular to the running of the vessel . in a separate session with both observers before the actual measurements , it was mutually agreed that the diameter was defined as the shortest cross - section through the center of artery . due to the high density of calcified plaques
, these may appear larger in ct images due to the so - called blooming artifact .
both observers were aware of these blooming artifacts and performed the diameter measurements while optimally correcting for these artifacts .
window / level settings were set to 750  hu ( width ) and 200  hu ( level ) , but the observers were allowed to adjust these settings .
we excluded the c7 segment of the intracranial ica because the diameter of the intracranial ica commonly reduces after the origin of the posterior communicating artery . including the diameter of the c7 segment may introduce pseudo - stenosis even in healthy arteries . following nguyen - huynh et al . and the wasid trial , we categorized the stenosis in the intracranial ica as either smaller or larger than a cutoff of 30% and 50% [ 10 , 17 ] . using the additional generated views
based upon the visible amount of high - intensity voxels in the ct images , the arteries were qualitatively labeled as absent , mild , moderate , or severe by two independent observers c.b.m and r.v.d.b . before the scoring sessions , a joint meeting was organized in which the observers agreed upon the method and measures of classification . a single observer l.b .
measured the volume of the calcium burden quantitatively with the 3mensio software of all arteries .
using the central lumen line as determined for the stenosis measurements , the segment of the central lumen line that contained calcifications was selected .
a region of interest was generated in this selected segment , which could be manually adjusted in the perpendicular view ( fig . 
since the calcifications were often closely adjacent to the skull base and the hu values of the skull are in the same range as the calcified plaques , the skull was manually removed from the region of interest .
a threshold value for calcifications was set at 420  hu to successfully separate the calcified voxels from the contrast - enhanced lumen .
subsequently , the software automatically calculated the volume of the voxels ( in cubic millimeters ) above this threshold in the defined region of interest ( fig . 
the semi - automatically segmented voxels were displayed in the software , subsequently checked for errors , and manually adjusted if required . because the volumetric calcium calculation is fully automatic and the delineation of the calcium from the skull was the only manual interaction , only limited user dependence was expected .
therefore , the interobserver variability of the calcium measurement was assessed using a subset of 20 consecutive patients .
the pearson correlation coefficient r was calculated to estimate the interobserver agreement . to estimate the statistical significance of the pearson correlation coefficient
, the p value was calculated using a two - tailed student s t test ; 95% confidence interval ( ci ) values were calculated .
using linear weighted kappa statistics , interobserver agreement of the categorized stenosis measurements ( cutoff values , 30% and 50% ) was determined .
interobserver differences in calcium volume classification ( absent , mild , moderate , or severe ) were presented with the linear weighted kappa . the following as standards for strength of agreement for the kappa coefficient were applied : < 0.00  =  poor , 0.000.20  =  slight , 0.210.40  =  fair , 0.410.60  =  moderate , 0.610.80  =  substantial , and 0.811.00  =  almost perfect .
the correlation of the semi - automatically quantified calcium volume measurement and calcification categorization of a single observer l.b . was presented as the mean    sd .
both arteries of each patient were considered independent from each other in the analysis . to evaluate possible causes of disagreement of the interobserver stenosis measurements , we selected stenosis measurements that differed more than 15% from the two observers .
we classified the errors as : ( a ) differences in reference diameter , ( b ) different position of the site of maximal stenosis , and ( c ) different measurement of the minimal diameter .
scatter plots of the differences in stenosis measurements as a function of minimal diameter or reference diameter were generated .
in our center , in the routine diagnostic workup , symptomatic patients suspected of having carotid artery stenosis are primarily evaluated by duplex sonography .
all consecutive patients who underwent cta on a 64-section ct scanner for carotid stenosis evaluation between april 1 , 2006 , and december 31 , 2008 , were included in the present study .
patients with a previous carotid intervention were excluded . within a case of an occlusion of the extracranial part of the ica and of an unreliable reference diameter of the intracranial ica ,
permission of the medical ethics committee was given for this retrospective analysis of anonymized patient data .
no informed consent was required because the evaluation of the images was performed outside the clinical setting .
cta was performed with a 64-slice scanner ( brilliance 64 , philips healthcare , best , the netherlands ) .
an 18-gauge intravenous catheter was placed in the antecubital vein , and 80  ml of contrast ( visipaque 320 , ge healthcare ) was infused at 4  ml / s after an initial injection delay depending on an attenuation of 150  hu in the aortic arch .
acquisition and reconstruction parameters were as follows : tube voltage , 120  kv ; effective mas , 265 ; pitch , 0.765 ; increment , 0.45  mm ; slice thickness , 0.9 or 1.5  mm .
the scan ranged from the aortic arch up to 3  cm above the sella turcica .
we used the warfarin  aspirin symptomatic intracranial disease ( wasid ) method for determining the degree of stenosis in the intracranial ica .
the wasid degree of stenosis is calculated as\documentclass[12pt]{minimal } 
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				 \begin{document}$$ \left ( { 1 - \frac{{smallest\,diameter}}{{reference\,diameter } } } \right ) \times 100\% $ $ \end{document } the reference diameter is measured at the widest straight part of the petrous segment of the ica .
if the entire petrous part of the ica is diseased , the most distal parallel part of the extracranial ica is used as reference . in the cta images ,
a central lumen line was created by a single trained observer lb using the 3mensio vascular software ( bilthoven , the netherlands ) .
the centerline started at the vertical running part of the petrous segment of the ica closely adjacent to the skull base , which lies in the carotid canal , up to the middle cerebral artery  anterior cerebral artery bifurcation ( fig . 
1views of the semi - automatic software . a volume rendering view of the ct data including the central lumen line starting at the vertical petrous segment adjacent to the skull base up to the middle cerebral artery  anterior cerebral artery bifurcation .
b a view perpendicular to the central lumen line used for diameter measurements ; the circle could be adjusted to measure the lumen diameter .
c stretched vessel view providing an overview of the whole running of the vessel , which includes lumen diameters as estimated by the software .
d illustration of the adjustment of the region of interest of the calcium volume measurement , which can be performed with the circular contour editor .
d calculated calcium voxels , which are the voxels with an intensity above a threshold value ( of 420  hu ) within the region of interest views of the semi - automatic software . a volume rendering view of the ct data including the central lumen line starting at the vertical petrous segment adjacent to the skull base up to the middle cerebral artery 
b a view perpendicular to the central lumen line used for diameter measurements ; the circle could be adjusted to measure the lumen diameter .
c stretched vessel view providing an overview of the whole running of the vessel , which includes lumen diameters as estimated by the software .
d illustration of the adjustment of the region of interest of the calcium volume measurement , which can be performed with the circular contour editor .
d calculated calcium voxels , which are the voxels with an intensity above a threshold value ( of 420  hu ) within the region of interest the centerline was used to generate additional views of the intracranial ica for the vessel analysis : a stretched vessel view , producing an overview of the whole running of the artery , and a perpendicular view , generating a view of the artery orthogonal to the centerline ( fig . 
subsequently , the observers independently measured the degree of stenosis , blinded for clinical information . to determine the degree of stenosis , the smallest diameter of intracranial ica up to the vertical running of segment c7 in the internal carotid artery classification of bouthillier   and a reference diameter of the intracranial ica was manually measured on the view perpendicular to the running of the vessel . in a separate session with both observers before the actual measurements , it was mutually agreed that the diameter was defined as the shortest cross - section through the center of artery . due to the high density of calcified plaques
, these may appear larger in ct images due to the so - called blooming artifact .
both observers were aware of these blooming artifacts and performed the diameter measurements while optimally correcting for these artifacts .
window / level settings were set to 750  hu ( width ) and 200  hu ( level ) , but the observers were allowed to adjust these settings .
we excluded the c7 segment of the intracranial ica because the diameter of the intracranial ica commonly reduces after the origin of the posterior communicating artery . including the diameter of the c7 segment may introduce pseudo - stenosis even in healthy arteries .
following nguyen - huynh et al . and the wasid trial , we categorized the stenosis in the intracranial ica as either smaller or larger than a cutoff of 30% and 50% [ 10 , 17 ] .
using the additional generated views , the intracranial ica was reviewed to detect calcifications in the artery wall . based upon the visible amount of high - intensity voxels in the ct images ,
the arteries were qualitatively labeled as absent , mild , moderate , or severe by two independent observers c.b.m and r.v.d.b . before the scoring sessions , a joint meeting was organized in which the observers agreed upon the method and measures of classification . a single observer l.b . measured the volume of the calcium burden quantitatively with the 3mensio software of all arteries . using the central lumen line as determined for the stenosis measurements ,
a region of interest was generated in this selected segment , which could be manually adjusted in the perpendicular view ( fig . 
since the calcifications were often closely adjacent to the skull base and the hu values of the skull are in the same range as the calcified plaques , the skull was manually removed from the region of interest .
a threshold value for calcifications was set at 420  hu to successfully separate the calcified voxels from the contrast - enhanced lumen .
subsequently , the software automatically calculated the volume of the voxels ( in cubic millimeters ) above this threshold in the defined region of interest ( fig . 
the semi - automatically segmented voxels were displayed in the software , subsequently checked for errors , and manually adjusted if required . because the volumetric calcium calculation is fully automatic and the delineation of the calcium from the skull was the only manual interaction , only limited user dependence was expected .
therefore , the interobserver variability of the calcium measurement was assessed using a subset of 20 consecutive patients .
the pearson correlation coefficient r was calculated to estimate the interobserver agreement . to estimate the statistical significance of the pearson correlation coefficient
, the p value was calculated using a two - tailed student s t test ; 95% confidence interval ( ci ) values were calculated . using linear weighted kappa statistics , interobserver agreement of the categorized stenosis measurements ( cutoff values , 30% and 50% ) was determined .
interobserver differences in calcium volume classification ( absent , mild , moderate , or severe ) were presented with the linear weighted kappa . the following as standards for strength of agreement for the kappa coefficient were applied : < 0.00  =  poor , 0.000.20  =  slight , 0.210.40  =  fair , 0.410.60  =  moderate , 0.610.80  =  substantial , and 0.811.00  =  almost perfect . the correlation of the semi - automatically quantified calcium volume measurement and calcification categorization of a single observer l.b .
to evaluate possible causes of disagreement of the interobserver stenosis measurements , we selected stenosis measurements that differed more than 15% from the two observers .
we classified the errors as : ( a ) differences in reference diameter , ( b ) different position of the site of maximal stenosis , and ( c ) different measurement of the minimal diameter .
scatter plots of the differences in stenosis measurements as a function of minimal diameter or reference diameter were generated .
eligible cta images of 88 patients were available . sixty percent of patients were male and the mean age was 67  years ( sd  =  12 ) . the baseline characteristics are presented in table  1 .
one artery was excluded because of an unreliable reference diameter of the ica.table 1demographic and clinical characteristics in patients ( n  =  88)characteristicvalueage ( years , mean ( sd))67 ( 12)gender  male53 ( 60% )  female35 ( 40%)index event  stroke39 ( 44% )  tia32 ( 36% )  amaurosis fugax14 ( 16% )  asymptomatic3 ( 3% )  ocular ischemic syndrome1 ( 1% ) demographic and clinical characteristics in patients ( n  =  88 ) the scatter plot of the stenosis measurements is shown in fig .  2 .
the pearson correlation coefficient r was 0.78 ( 95% ci  =  0.710.83 , p  <  0.001 ) . with a cutoff at 30% stenosis , the interobserver agreement measured by the linear weighted kappa was 0.45 ( 95% ci  =  0.280.62 ) . with a taken 50% stenosis cutoff ,
the mean difference of the degree of stenosis measurements was 9.2    7.7% .
the interobserver agreement expressed as the linear weighted kappa of the qualitative categorization of the calcium volume was 0.62 ( 95% ci  =  0.540.70).fig .
2scatter plot of the stenosis measurement of the two observers with the cutoff values at 30% and 50% scatter plot of the stenosis measurement of the two observers with the cutoff values at 30% and 50% the relation between the qualitative and quantitative calcium score is illustrated in fig . 
the agreement of the quantitative and qualitative scoring of the calcium volume of arteries labeled as absent calcium was perfect with a mean volume of 0    0  mm .
the mean calcium score of the mildly calcified vessels was 31    37  mm , and the vessels that were qualified as moderate calcified vessels had a mean calcium score of 113    76  mm .
the vessels that were qualified as severe calcified had a mean of 288    162  mm calcium .
the interobserver agreement of the semi - automatically quantitative calcium score was excellent , with a pearson s correlation coefficient of 0.99 ( p  <  0.01 ) with a 95% limit of agreement of 0.3    8.5  mm.fig .
the boxes show the mean quantitative calcium score ( in cubic millimeters ) per calcification category ; the whiskers show the standard deviation .
the mean quantitative calcium scores in the absent , mild , moderate , and severe groups are 0    0 , 31    37 , 113    76 , and 288    162  mm , respectively correlation between the qualitative and quantitative calcium score .
the boxes show the mean quantitative calcium score ( in cubic millimeters ) per calcification category ; the whiskers show the standard deviation .
the mean quantitative calcium scores in the absent , mild , moderate , and severe groups are 0    0 , 31    37 , 113    76 , and 288    162  mm , respectively eighty - five percent of the measured degree of stenosis had a difference in stenosis measurement of 15% or less between the two observers . of the arteries with larger differences , 7 had a relatively large difference in reference diameter , 15 arteries had a different position of the site of maximal degree of stenosis , and a different measurement of the minimal diameter was observed in 9 arteries .
absolute differences in the stenosis measurements did not depend on the size of mean reference diameter or mean minimal diameter ( fig . 
the horizontal lines express the mean difference ( 4.65 ) and the mean    2 sd difference in stenosis measurements as a function of mean reference or minimal diameter .
the horizontal lines express the mean difference ( 4.65 ) and the mean    2 sd
eighty - five percent of the measured degree of stenosis had a difference in stenosis measurement of 15% or less between the two observers .
of the arteries with larger differences , 7 had a relatively large difference in reference diameter , 15 arteries had a different position of the site of maximal degree of stenosis , and a different measurement of the minimal diameter was observed in 9 arteries .
absolute differences in the stenosis measurements did not depend on the size of mean reference diameter or mean minimal diameter ( fig . 
the horizontal lines express the mean difference ( 4.65 ) and the mean    2 sd difference in stenosis measurements as a function of mean reference or minimal diameter .
the horizontal lines express the mean difference ( 4.65 ) and the mean    2 sd
we have presented a novel semi - automatic application to quantify the intracranial ica stenosis and calcification on cta images . a good interobserver agreement for the stenosis measurement
the qualitative classification of the calcifications in the intracranial ica agrees well with the quantitative calcium volume measurements .
there is increasing evidence that the quantitative assessment of intracranial atherosclerotic disease is of great importance for prognostics and treatment decision .
since the 1980s , it is known that stenosis of the intracranial ica carries an increased risk of stroke [ 19 , 20 ] . in a systematic review to assess the impact of potential risk factors other than compromised cerebral blood flow , it was found that patients with intracranial ica stenosis or occlusion have a higher rate of recurrent stroke ( rate ratio , 1.09 ; 95% ci  =  1.051.14 ) than patients with extracranial carotid or middle cerebral artery stenosis or occlusion . the incidence of symptomatic cerebral ischemia as results of in - tandem stenosis (
extracranial ica stenosis with ipsilateral intracranial ica stenosis ) has been estimated between 20% and 50% .
supported the idea that in symptomatic carotid disease , deep watershed infarcts result from hemodynamic impairment secondary to severe lumen stenosis or from microembolism secondary to plaque inflammation .
decision making for treatment may need to take the hemodynamic situation of in - tandem stenosis into account .
carotid endarterectomy has been reported to have an increased perioperative risk in the presence of carotid siphon stenosis [ 24 , 25 ] .
have shown that the characteristics of intracranial stenoses determine the feasibility of the endovascular procedures .
alternative treatments have been suggested such as simultaneous stenting of intra- and extracranial carotid artery or a combined extracranial endarterectomy and intracranial angioplasty [ 27 , 28 ] .
the interobserver variability of intracranial stenosis measurement has been addressed by a few previous studies . in a group of patients suspected of acute cerebral ischemia , nguyen - huynh et al .  
measured the intracranial stenosis in which 93.3% and 90.5% of the vessel segments had an error smaller than 10% for dsa and maximum intensity profile thin slab images of cta , respectively . in our study
this difference in performance may be caused by the inclusion of multiple intracranial vessel segments in the stenosis measurement instead of only the most severe .
.   showed a high interobserver agreement for the stenosis measurement in 28 patients using the nascet criteria in 24 segments per patient , resulting in a pearson correlation coefficient of 0.95 ( 95% ci  =  0.930.96 ) .
.   showed a good interobserver agreement with a kappa of 0.79 ( 95% ci  =  0.551.00 ) in intracranial stenosis categorization arteries excluding the carotid siphon in the cta images of 50 patients .
the somewhat lower correlation values that we find may indicate that the degree of stenosis of the intracranial ica is more difficult to measure than the other intracranial vessel segments . in a previous study ,
the interobserver agreement of a manual stenosis measurement of the extracranial ica was calculated with a comparable pearson correlation coefficient , r  =  0.86 ( 95% ci  =  0.760.92 ) , on the same patient group .
similar results were obtained with correlation coefficients of 0.60 , 0.770.79 , and 0.90 [ 3133 ] . for ( semi-)automated analysis , the correlation improved with correlation coefficients of 0.96 ( 95% ci  =  0.950.96 ) , 0.93 , and 0.90 [ 30 , 31 , 34 ] .
kappa values for stenosis categorization of the extracranial stenosis vary considerably , ranging from 0.48 to 0.93 and from 0.65 to 0.93 for manual and automated stenosis measurements , respectively .
the kappa values that we determined for the intracranial stenosis measurements are in the low end of this range .
the somewhat lower kappa values can be associated with scattering of the stenosis measurements around the cutoff points of 30% and 50% stenosis .
measurement of the calcification in the intracranial ica has been addressed in previous studies with either qualitative categorization [ 35 , 36 ] or quantitative measurements [ 37 , 38 ] .
however , the interobserver agreement and correlation between quantitative and qualitative calcium measurements was not considered previously .
only the separation of calcium in the vessel wall from the skull was performed manually .
therefore , a high interobserver agreement was expected and a smaller population of 20 patients was used to assess this agreement . with a pearson s correlation of 0.99 , it was shown that the interobserver agreement was indeed excellent .
the calculated calcium volume may depend on the threshold that was used . in the literature , this threshold varies severely from 130  hu in non - contrast ct images of the coronary arteries   up to 800  hu in the cta of the aortic root . the threshold should be set to a value that accurately separates calcium from the contrast - enhanced lumen .
the threshold of 420  hu was empirically determined in a subset of the images before the actual measurements were performed .
we observed a good correlation of the qualitative calcium scoring with the quantitative calcium volume measurements , which is reassuring .
this means that no bias can be expected when either one is chosen in diagnostic studies . in this study
, we addressed the quantitative volume measurements because we expect that intracranial calcium volume will play an important role in future risk assessment in patients with stroke .
. a smaller interobserver agreement of these measurements as compared with extracranial stenosis measurements could be expected . with the small diameters of the intracranial ica with reference diameter ranging between 2.3 and 6.7  mm , in combination with the limited resolution of about 0.5  mm , small errors in a measurement
however , in our study , no direct relation between smaller reference diameter or minimal diameter with difference in stenosis measurement was found . along the course of the intracranial ica ,
there are relatively large variations of the diameter , which makes it more difficult to obtain reproducible reference diameters .
we tried to reduce interobserver variations by choosing the site of the largest diameter in the horizontal petrous part of the internal carotid artery as the reference diameter .
in a recent study , it was shown that volume rendering and maximum - intensity projection techniques provide the optimal interobserver agreement for intracranial stenosis measurement . however , for intracranial ica , these techniques are suboptimal due to the present adjacent skull .
the software used in this study produced a stretched vessel view based upon the centerline .
this view enabled an overview of the whole vessel in a single view , which facilitated the identification of the area with the smallest diameter .
the cross - section view of the vessel made it possible to accurately measure the diameter .
furthermore , hong et al .   found a correlation between the degree of carotid siphon calcification and the occurrence of lacunar infarction .
the software enabled a rotation around the vessel , facilitating an overview of the calcification of the vessel .
because the intracranial ica is closely adjacent to the skull and the intravascular contrast enhancement is in the same range of hu as the calcifications , it is difficult to measure the calcifications . with the software
, we could manually adjust the region of interest by removing the skull out of this region of interest .
a limitation of the present study is that the quantitative calcium volume measurement is not fully automated and can be rather labor - intensive due to the manual editing .
the analysis required placement of seed points for the generation of centerlines , manual diameter measurements , and a separation of the calcifications from the skull .
it was estimated that it lasted approximately 34  min per vessel to generate the central lumen line per vessel .
the semi - automatic quantitative calcium measurement was performed in approximately 10  min per vessel .
another limitation of the current study is that we could not estimate the diagnostic accuracy by comparison with a gold standard because performing dsa in all patients , for research purposes , is not ethical anymore .
however , the accuracy in stenosis measurement of intracranial arteries in cta as compared with the gold standard dsa has previously been demonstrated in previous studies [ 1013 ] .
since the actual measurement was comparable to these previous studies , we assume a similar accuracy of the stenosis measurements in this study . also , the accuracy of the calcium volume measurement was not assessed .
calcium volume measurements are based on straightforward thresholding techniques in which the voxels with intensities higher than a threshold are counted and weighted .
this technique was introduced in the early 1990s and is routinely performed in clinical practice to assess the calcium burden in coronary arteries .
the validation of this technique is still subject of research even 20  years since its introduction . in 1995 , rumberger et al .
confirmed an intimate relationship with histologically observed atherosclerotic plaque volume and calcium volume scores on ct images [ 44 , 45 ] .
since then , it was shown that calcium score is a good indicator of the extent of atherosclerotic disease .
more recently , it was shown that calcium volume measured on cta also strongly correlates with the original agatston score on non - enhanced ct images .
the spatial resolution of cta is limited with a 0.9-mm z - axis resolution and an average axial resolution of 0.3  mm .
in small vessels with a diameter <3  mm , a small difference in diameter measurement may result in a considerable difference in degree of stenosis .
although both observers have more than 10  years experience in measuring the degree of stenosis in cta images , the intracranial ica stenosis is not measured on a routine basis in cta , and this study may suffer from a learning curve effect .
we expect that advanced image processing algorithms may decrease the analysis time and increase the interobserver agreement .
for example , matched masked bone elimination results in an image of the intracranial artery tree without the skull .
on such an image , it is easier to segment the arteries and to automatically generate a centerline .
subsequently , the position of minimal diameter and the amount of calcification could be determined .
it has been shown that such automations result in an increase of interobserver agreement and a reduction in analysis time .
we have presented and evaluated a semi - automatic method to quantitatively measure the degree of stenosis and the calcification volume in the intracranial ica .
a good interobserver agreement was obtained for the stenosis measurements and a substantial interobserver agreement for qualitative calcium categorization . by providing reproducible quantitative assessment of intracranial internal carotid disease
this article is distributed under the terms of the creative commons attribution noncommercial license which permits any noncommercial use , distribution , and reproduction in any medium , provided the original author(s ) and source are credited .
 introductionintracranial carotid artery atherosclerotic disease is an independent predictor for recurrent stroke . 
 however , its quantitative assessment is not routinely performed in clinical practice . in this diagnostic study , we present and evaluate a novel semi - automatic application to quantitatively measure intracranial internal carotid artery ( ica ) degree of stenosis and calcium volume in ct angiography ( cta ) images.methodsin this retrospective study involving cta images of 88 consecutive patients , intracranial ica stenosis was quantitatively measured by two independent observers . 
 stenoses were categorized with cutoff values of 30% and 50% . 
 the calcification in the intracranial ica was qualitatively categorized as absent , mild , moderate , or severe and quantitatively measured using the semi - automatic application . 
 linear weighted kappa values were calculated to assess the interobserver agreement of the stenosis and calcium categorization . 
 the average and the standard deviation of the quantitative calcium volume were calculated for the calcium categories.resultsfor the stenosis measurements , the cta images of 162 arteries yielded an interobserver correlation of 0.78 ( p  <  0.001 ) . 
 kappa values of the categorized stenosis measurements were moderate : 0.45 and 0.58 for cutoff values of 30% and 50% , respectively . 
 the kappa value for the calcium categorization was 0.62 , with a good agreement between the qualitative and quantitative calcium assessment.conclusionsquantitative degree of stenosis measurement of the intracranial ica on cta is feasible with a good interobserver agreement ica . 
 qualitative calcium categorization agrees well with quantitative measurements . 
Introduction
Methods
Study population
CT imaging protocol
Degree of stenosis in the intracranial ICA
Intracranial calcium volume
Statistical analysis
Error analysis
Results
Error analysis
Discussion
Conclusion
Conflict of interest
Open Access