microorganisms play an essential role in pulpal and periapical diseases . therefore , the purpose of endodontic treatment is to eliminate microorganisms from the root canal system and to prevent recontamination by creating a seal barrier between the oral microflora and the root canal system and periapical tissue . in reality , creating a fluid - tight apical , lateral and coronal seal is necessary to prevent recontamination and long - term clinical success . several root filling materials and techniques
have been developed with the purpose of obtaining a tight root canal seal . ideally , the root canal filling should be a complete , homogeneous mass that fills the prepared canal completely .
mineral trioxide aggregate ( mta ) has a variety of potential uses , including a root canal filling material [ 48 ] .
studies have demonstrated the encouraging regeneration of periradicular tissues , such as periodontal ligament , bone and cementum when mta was used in endodontic procedures .
there are also several reports of its superior biocompatibility with periodontal tissues , excellent sealing ability in the presence of moisture   and appropriate mechanical properties as apical sealing material .
these encouraging outcomes from in vivo and in vitro studies have prompted many clinicians to consider the use of mta as a root end filling material and as a material suitable for repairing perforations and performing apexification .
mta has been used for apexification of immature roots instead of calcium hydroxide [ ca(oh)2 ] because it facilitates the normal periradicular architecture by inducing hard tissue barriers .
recently , great interest has been focused on the evolution of portland cement ( pc ) as an alternative to mta and several studies have compared both materials .
pc differs from mta by the absence of bismuth ions   and the presence of potassium ions .
both materials have comparable antibacterial activity   and almost identical properties macroscopically , microscopically and by x - ray diffraction analysis .
it has also been shown that pc and mta have similar effects on pulpal cells when used for direct pulp capping in rat teeth .
taking into account the low cost and apparently similar properties of pc in comparison to mta , it is reasonable to consider pc as a possible substitute for mta in endodontic applications .
the purpose of this in vitro study was to evaluate the apical sealability of white - colored mta ( wmta ) and grey - colored pc ( gpc ) when used alone or as a sealer with gutta - percha points in root canal filling .
eighty - one recently extracted for several reasons , single - rooted , permanent human teeth were selected .
the teeth with open apical cracks and resorptive defects were excluded and stored in saline solution until they were used .
after that , the teeth were cleaned and x - rays were taken to assess the absence of anatomical anomalies in the canal system .
after creation of the coronal access , canal length was determined by placing a # 10 stainless steel k file ( dentsply maillefer , ballaigues , switzerland ) through the canal space until it could be visualized exiting the apical foramen .
the coronal and middle two thirds of these canals were prepared sequentially with size 2 , 3 , and 4 gates - glidden burs ( dentsply maillefer , ballaigues , switzerland ) and the apical third of the canals was prepared with hand files ( dentsply maillefer , ballaigues , switzerland ) using the step - back technique .
after preparation , the canals were irrigated with 1 ml of 17% edta solution ( md - cleanser , meta biomed , cheongju , korea ) for 60 seconds to remove the smear layer , because it contains bacteria and may proliferate into the dentinal tubules which may serve as a reservoir of microbial irritants .
it may act as a substrate for bacteria , allowing their deeper penetration in the dentinal tubules .
the canals were then dried with sterile paper points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) .
the teeth were randomly divided into five equal groups of 15 teeth each , according to the root canal filling material used : 
 group1 : wmta alone ( proroot mta , dentsply , tulsa , usa).group2 : gpc alone ( syrian company , tartuos , syria ) was previously sterilized with dry heat cycle 170c for one hour.group3 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with wmta ( proroot mta , dentsply , tulsa , usa).group4 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with gpc ( syrian company , tartuos , syria).group5 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa)laterally condensed with ah26 sealer ( dentsply , detrey gmbh , konstanz , germany ) which was considered as the control group because it is a standard material in root canal filling .
group2 : gpc alone ( syrian company , tartuos , syria ) was previously sterilized with dry heat cycle 170c for one hour .
group3 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with wmta ( proroot mta , dentsply , tulsa , usa ) .
group4 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with gpc ( syrian company , tartuos , syria ) .
group5 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa)laterally condensed with ah26 sealer ( dentsply , detrey gmbh , konstanz , germany ) which was considered as the control group because it is a standard material in root canal filling . to test the nail varnish sealability and the penetrability of the dye in the root canal
, three roots were obturated using gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with zinc oxide - eugenolsealer(z.o.e ) ( s.s.white , artigos dentrios ltda , brasileira ) and considered as a positive control group .
in addition , three roots were left empty without obturation and considered as the negative control group . in the third and fourth groups , gutta - percha points were used to facilitate retreatment .
mta and ah26 were prepared according to manufacturer recommendations ; pc was mixed by adding distilled water to the powder at the same proportion recommended by the manufacturer of mta . in the first and second groups ,
the mixed cements were applied to the canal walls with a lentulo spiral at slow speed and the fresh mix was then incrementally packed in the root canals using measured figer endodonticpluggers(dentsply , maillefer , swiss ) . to control placement and manipulation of the mta and pc , large paper points (
alpha - dent , north hamlin avenue , lincolnwood , usa ) were used to blot the excess moisture from the cement and to assist in the vertical condensation of mta and pc . in the third , fourth and fifth groups ,
the mixed cements were applied to the canal walls with a lentulo spiral at slow speed and the teeth were filled by the lateral condensation technique .
excess gutta - percha and cement were removed with a heated plugger and vertically condensed .
each tooth was radiographed to confirm the length and density of the root canal obturation .
after that , glass ionomer cement ( gic ) ( restomolar , dencare , england , uk eec ) was placed in the access opening of all groups and the teeth were stored in 100% humidity at 37c for 7 days to allow the obturation materials to set .
next , all groups were removed from the incubator , dried and then the teeth surfaces were coated with two layers of nail varnish leaving the apical foramen of the root exposed .
the negative control group was completely coated with two layers of nail varnish , including the apical foramen .
all specimens were immersed in 2% methylene blue solution ( 100% humidity at 37c ) for 24 hours .
the teeth were sectioned longitudinally in a buccolingual direction with a diamond straight fissure bur ( 1.6 mm in diameter and 8 mm in length ) using a high speed hand piece with water coolant .
then , the sections were examined by a stereomicroscope ( x 20 magnification ) ( meiji techno , model skt 41323 , japan ) for evaluation of linear dye penetration at the tooth - material interface .
the linear apical leakage was measured from the distance of the anatomical apex to the maximum extent of dye penetration in the coronal direction .
the greatest extent of linear dye penetration was recorded using electronic digital caliper ( lezaco , china , measuring range 0150 mm ) .
escobar et al s   criteria was used to evaluate the infiltration proportions : 
 0-infiltration loss ( dye penetration 0  < 1.5 mm).1-simple infiltration ( dye penetration 1.5  3 mm).2-medium infiltration ( dye penetration > 3 mm ) . 0-infiltration loss ( dye penetration 0  < 1.5 mm ) .
statistical analysis of the data was accomplished using a kruskal - wallis test at a significance level of 0.05 .
we also used statistical tests that depended on the arithmetic means and the standard deviations to obtain more clear and precise results .
so , one - way anova analysis was used to compare the linear dye penetration values at a significance level of 0.05 according to the type of the variables and their distribution , we used the parametric one - way anova or non - parametric kruskal - wallis test for statistical analysis .
eighty - one recently extracted for several reasons , single - rooted , permanent human teeth were selected .
the teeth with open apical cracks and resorptive defects were excluded and stored in saline solution until they were used .
after that , the teeth were cleaned and x - rays were taken to assess the absence of anatomical anomalies in the canal system .
after creation of the coronal access , canal length was determined by placing a # 10 stainless steel k file ( dentsply maillefer , ballaigues , switzerland ) through the canal space until it could be visualized exiting the apical foramen .
the coronal and middle two thirds of these canals were prepared sequentially with size 2 , 3 , and 4 gates - glidden burs ( dentsply maillefer , ballaigues , switzerland ) and the apical third of the canals was prepared with hand files ( dentsply maillefer , ballaigues , switzerland ) using the step - back technique .
after preparation , the canals were irrigated with 1 ml of 17% edta solution ( md - cleanser , meta biomed , cheongju , korea ) for 60 seconds to remove the smear layer , because it contains bacteria and may proliferate into the dentinal tubules which may serve as a reservoir of microbial irritants .
it may act as a substrate for bacteria , allowing their deeper penetration in the dentinal tubules .
the canals were then dried with sterile paper points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) .
the teeth were randomly divided into five equal groups of 15 teeth each , according to the root canal filling material used : 
 group1 : wmta alone ( proroot mta , dentsply , tulsa , usa).group2 : gpc alone ( syrian company , tartuos , syria ) was previously sterilized with dry heat cycle 170c for one hour.group3 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with wmta ( proroot mta , dentsply , tulsa , usa).group4 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with gpc ( syrian company , tartuos , syria).group5 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa)laterally condensed with ah26 sealer ( dentsply , detrey gmbh , konstanz , germany ) which was considered as the control group because it is a standard material in root canal filling .
group2 : gpc alone ( syrian company , tartuos , syria ) was previously sterilized with dry heat cycle 170c for one hour .
group3 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with wmta ( proroot mta , dentsply , tulsa , usa ) .
group4 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with gpc ( syrian company , tartuos , syria ) .
group5 : gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa)laterally condensed with ah26 sealer ( dentsply , detrey gmbh , konstanz , germany ) which was considered as the control group because it is a standard material in root canal filling . to test the nail varnish sealability and the penetrability of the dye in the root canal
, three roots were obturated using gutta - percha points ( alpha - dent , north hamlin avenue , lincolnwood , usa ) laterally condensed with zinc oxide - eugenolsealer(z.o.e ) ( s.s.white , artigos dentrios ltda , brasileira ) and considered as a positive control group . in addition , three roots were left empty without obturation and considered as the negative control group . in the third and fourth groups , gutta - percha points were used to facilitate retreatment .
mta and ah26 were prepared according to manufacturer recommendations ; pc was mixed by adding distilled water to the powder at the same proportion recommended by the manufacturer of mta . in the first and second groups ,
the mixed cements were applied to the canal walls with a lentulo spiral at slow speed and the fresh mix was then incrementally packed in the root canals using measured figer endodonticpluggers(dentsply , maillefer , swiss ) . to control placement and manipulation of the mta and pc , large paper points (
alpha - dent , north hamlin avenue , lincolnwood , usa ) were used to blot the excess moisture from the cement and to assist in the vertical condensation of mta and pc . in the third , fourth and fifth groups ,
the mixed cements were applied to the canal walls with a lentulo spiral at slow speed and the teeth were filled by the lateral condensation technique .
excess gutta - percha and cement were removed with a heated plugger and vertically condensed .
each tooth was radiographed to confirm the length and density of the root canal obturation .
after that , glass ionomer cement ( gic ) ( restomolar , dencare , england , uk eec ) was placed in the access opening of all groups and the teeth were stored in 100% humidity at 37c for 7 days to allow the obturation materials to set .
next , all groups were removed from the incubator , dried and then the teeth surfaces were coated with two layers of nail varnish leaving the apical foramen of the root exposed .
the negative control group was completely coated with two layers of nail varnish , including the apical foramen .
all specimens were immersed in 2% methylene blue solution ( 100% humidity at 37c ) for 24 hours .
the teeth were sectioned longitudinally in a buccolingual direction with a diamond straight fissure bur ( 1.6 mm in diameter and 8 mm in length ) using a high speed hand piece with water coolant . then
, the sections were examined by a stereomicroscope ( x 20 magnification ) ( meiji techno , model skt 41323 , japan ) for evaluation of linear dye penetration at the tooth - material interface .
the linear apical leakage was measured from the distance of the anatomical apex to the maximum extent of dye penetration in the coronal direction .
the greatest extent of linear dye penetration was recorded using electronic digital caliper ( lezaco , china , measuring range 0150 mm ) .
escobar et al s   criteria was used to evaluate the infiltration proportions : 
 0-infiltration loss ( dye penetration 0  < 1.5 mm).1-simple infiltration ( dye penetration 1.5  3 mm).2-medium infiltration ( dye penetration > 3 mm ) .
statistical analysis of the data was accomplished using a kruskal - wallis test at a significance level of 0.05 .
we also used statistical tests that depended on the arithmetic means and the standard deviations to obtain more clear and precise results .
so , one - way anova analysis was used to compare the linear dye penetration values at a significance level of 0.05 according to the type of the variables and their distribution , we used the parametric one - way anova or non - parametric kruskal - wallis test for statistical analysis .
the scores for apical dye penetration evaluation by escobar et al criteria are shown in table i. the extent of dye penetration in millimeters , the means and the standard deviations for each group are listed in table ii .
apical dye penetration in the materials of five groups are seen in figures [ i  vi ] .
the three roots in the positive control group showed dye penetration throughout the entire length of the root canal , whereas all three roots in the negative control group showed no evidence of dye penetration which proves that the conditions of the tests are correct .
data for apical leakage were subjected to a kruskal - wallis test , there were no statistically significant differences among the materials of five groups ( 2 = 4.672 , df = 4 , p = 0.323 , p - value > 0.05 ) .
data were also subjected to one - way anova analysis , there were no statistically significant differences among the materials of five groups ( f = 2.175 , p = 0.081 , p - value > 0.05 ) .
three - dimensional hermetic sealing of the root canal is one of the main goals of endodontic treatment and is essential for preventing apical and coronal leakage in the root canal system .
inadequate filling may result in fluid movement into the filling defects , inducing a periapical chronic inflammatory reaction and compromising the treatment success .
several test methods have been described to evaluate the sealing quality of obturated root canals .
dye penetration studies are commonly used because they are easy to accomplish and do not require sophisticated materials .
methylene blue dye was used in this study because it easily allows quantitative measurement of the extent of dye penetration by linear measurement techniques .
its molecular size is similar to bacterial by products such as butyric acid which can leak out of infected root canals to irritate periapical tissues .
cleaning the dentinal surface by removing the smear layer is an essential step in the process of successful root canal treatment . in the present study ,
the smear layer was removed by edta in order to improve the adaptation of the cement to the canal wall .
removing the smear layer does not increase the leakage because most in vitro and in vivo studies that evaluated mta in root canal filling have used edta for removing the smear layer .
if smear layer removal is not implemented , it does not appear to affect the sealability of mta and its presence might actually improve the seal over time .
the apical microleakage of mta is less when the smear layer is present compared to when it is absent .
it has been speculated that the smear layer might act as a ( coupling agent ) that might enhance mta bonding to root canal dentin .
mta is a type of hydraulic cement that can set in the presence of water .
the moist environment caused by the smear layer might have a positive effect on the adaptation of mta to the root canal wall .
because the present data are inconclusive regarding smear layer removal , clinicians might choose to leave the smear layer in selected cases and fill canals with mta without apparently compromising the outcome .
the results of this study showed that the materials of five groups presented similar apical sealability .
this has resulted in a significant body of research   that shows pc appears to be a useful substitute for mta .
danesh et al   showed that mta displayed superior material properties compared topc , which was significantly more soluble , reached lower microhardness values and was less radiopaque .
the lack of radiopacity in pure pc is due to the absence of bismuth , a chemically inert radiopacifier in its composition .
however , our results differ from those of vizgirda et al , who reported that the apical seal produced by laterally condensed gutta - percha with roth s 801 sealer was superior to that produced by gutta - percha with mta .
the difference in results could be attributed to the following reasons : ( a ) in our study , dye penetration was evaluated by buccolingual longitudinal sections method as opposed to the teeth clearing technique ; ( b ) human teeth were used here instead of bovine teeth ; ( c ) besides ; the difference could be attributed to the type of sealer used ; ( d ) canal preparation was accomplished by using the crown - down technique as opposed to step - back canal preparation with lightspeed instruments and saline ; ( e ) the apical portion of the root canal was prepared to a # 80 with light speed nickel - titanium rotary instruments ; ( f ) without removing the smear layer .
the findings of the present study are in contrast with those obtained by tabrizizadeh et al .
they assessed the apical sealability of mta alone and laterally condensed gutta - percha with ah26 , and showed that the canal obturation with gutta - percha and ah26 sealer may provide a better apical seal compared with mta alone .
the difference could be attributed to the method of dye penetration evaluation ( teeth clearing ) , besides the smear layer was not removed .
et al   used human saliva penetration and showed that mta alone is better than vertically condensed gutta - percha and kerr pulp canal sealer ewt ( zinc oxide - eugenol based sealer ) .
the difference could be attributed to the leakage measured by bacterial penetration as opposed to dye penetration , the type of sealer used and the condensation technique .
clinical support for the use of mta as an obturating material , however , was presented in some case reports . in a case report
, osullivan and hartwell   used mta alone as the obturating material for the root canal system of a retained primary second molar . at the 4-month follow - up ,
the patient was asymptomatic , clinical findings were within normal limits and there was evidence of radiographic healing . in another case report , mta separately was used for obturation of the root canal system of two mature mandibular central incisors with apical periodontitis .
a 2-year follow - up radiographic examination demonstrated the dramatic regeneration of the periradicular tissue .
extrapolation of the results of this in vitro study into clinical reality must be performed with caution . post space preparation is often required immediately following root canal obturation with mta or pc each separately .
in addition , retrieving the set mta or pc alone from the root canal is difficult if nonsurgical retreatment is indicated .
therefore , it is recommended that root canal filling with mta or pc separately should be limited to selected cases such as one - visit apexification and situations where future nonsurgical retreatment is nonfeasible or may not render better tooth prognosis .
further investigation should be conducted to determine whether some physical properties of mta and pc or the technique for its placement could be modified to facilitate its use as a root canal filling material .
within the limitations of this study , both wmta and gpc alone produced the apical sealability equal to that produced by gutta - percha points and lateral condensation techniques .
 objective : one of the principle purposes of root canal obturation is to obtain hermetic sealing of the root canal system . according to the development of technology , 
 many materials are now used in root canal filling . 
 an in vitro dye leakage study was performed to evaluate the apical sealability of white - colored mineral trioxide aggregate ( wmta ) and gray - colored portland cement ( gpc ) when used alone or as a sealer with gutta - percha points in root canal filling.materials and methods : seventy - five single - rooted extracted human teeth were used in this study . after cleaning and shaping , 
 the teeth were randomly divided into five equal groups of 15 teeth each based on the root canal filling material used ; group 1 , ( wmta ) alone ; group 2 , ( gpc ) alone ; group 3 , ( gutta - percha points + wmta ) ; group 4 , ( gutta - percha points + gpc ) ; group 5 , ( gutta - percha points + ah26 ) . 
 methylene blue was used to determine the apical leakage . 
 after sectioning the teeth longitudinally , linear dye penetration was measured with a caliper under the stereomicroscope . 
 data were analyzed by kruskal - wallis and one - way anova tests with ( p  0.05 ) as the level of significance.results:the results showed that there were no statistically significant differences among the materials of five groups.conclusion:(wmta ) alone , ( gutta - percha points + wmta ) , ( gpc ) alone and ( gutta - percha points + gpc ) may be used in the root canal filling . 
INTRODUCTION
MATERIALS AND METHODS
Selection of teeth
Preparation
Obturation
Assessment of apical leakage
Data Management
RESULTS
DISCUSSION
CONCLUSION