are the adhesive systems , due to their high bond strength to human enamel1 and low incidence of enamel fracture on bracket removal .
the use of such material is only possible after the substrate acid etching and application of an adhesive coating to bond the composite resin or brackets to enamel .
when acid conditioner is presented in isolated bottles than primer and adhesives it constitutes the " etch - and - rinse " adhesive systems .
the acid etching demineralizes the enamel prisms , creating a porous surface through which the adhesive can penetrate , resulting on a micro - mechanical retention .
such systems simplify the steps of acid etching and primer in a single step , transforming it into a self - etching primer .
the self - etching bond procedure leads to less technique sensitivity ( i.e. , no rinsing step)9,16,23 and fewer operative steps .
contamination during the adhesion process reduces the longevity of the bonding materials , once it can alter the material properties6 , substrate surface21 and cause problems in the bonding process19
. in orthodontics , saliva contamination could lead to debonding of metallic brackets increasing treatment duration .
the aim of this study was to verify the influence of saliva contamination on the bond strength and failure pattern of different adhesive systems used with a light - cured composite resin for bonding of metallic orthodontic brackets to human enamel .
the sample consisted of 72 extracted human healthy third molars which were randomly divided into four groups and stored in distilled water until the bonding procedure .
the adhesive systems used and its components , composition and manufacturer are shown in figure 1 .
enamel surface of each specimen was polished with pumice at low speed , thoroughly washed with distilled water and dried . the bonding procedure for each group
each adhesive system was tested under 2 different enamel conditions : with no contamination and contaminated with saliva . in t , a and x groups , the adhesive systems were applied to the enamel surface in accordance with manufacturer 's instructions . in ts , as and xs groups , saliva was applied to enamel surface immediately before the etching ( ts ) and acidic primer ( as and xs ) steps .
tooth contamination was done with natural and stimulated human saliva from a male donor , who was instructed to brush his teeth and do not eat for 1 h before saliva collection .
an amount of 3 l of saliva was applied to each tooth with a microbrush until the surface was totally contaminated for approximately 5 ( 1 ) s. after saliva contamination , the enamel surface was not blown off and the adhesive systems were applied . in all groups , specimens were light - cured for 20 s with a light - curing unit ( curing light xl 3000 , 3 m espe , st paul , mn , usa ) with light intensity of 550 mw / cm , as measured with a radiometer ( model 100 , demetron , danbury , ct , usa ) .
the metallic brackets ( morelli , sorocaba , sp , brazil ) were bonded on the central portion of the specimens surface with a light - cured resin ( transbond xt - 3m / unitek co. , minneapolis , mn , usa ) , according to the manufacturer 's instructions , and cured at a distance of 5 mm for 40 s. after bonding the brackets , the specimens were stored in distilled water at 37c for 24 h. each specimen was measured with a digital caliper before de test .
the shear bond strength test was performed in a universal testing machine ( emic dl2000 , so jos dos pinhais , pr , brazil ) with a crosshead speed of 1 mm / min with a guillotine device .
after bracket debonding , the adhesive remnant index ( ari ) was verified under an optical microscope at 40x magnification21 .
the failure patterns were classified according to the following scores : 0 = no adhesive remaining on the tooth in the bonding area ; 1 = less than half of the adhesive remaining ; 2 = more than half of the adhesive remaining ; and 3 = all adhesive remaining , with a distinct impression of the bracket mesh .
in addition to descriptive statistical analysis ( mean and standard deviation ) , the kolmogorof - smirnoff test was performed to verify the normality of the shear bond strength data .
the analysis of the data was completed with anova and multiple comparisons tukey 's test to determine whether difference existed among the groups .
the mean values of shear bond strength are showed in table 1 . when compared the dry condition and saliva contamination for each material , no statistical significant difference were found . however , the group t ( " etch and rinse " adhesive system ) , in dry condition , showed significantly higher bond strength than self - etching adhesive systems contaminated groups ( as , xs ) ( p<0.05 ) .
t= transbond xt ; ts= transbond with contamination ; a= adhese ; as= adhese with contamination ; x= xeno iii ; xs= xeno iii with contamination .
the kruskal - wallis test showed statistically significant difference in the ari scores ( p<0.001 ) .
the mann - whitney pairwise test showed that contamination for the same adhesive system did not produce statistical significance .
statistically significant difference was observed between the self - etching and " etch and rinse " adhesive systems ( figure 3 ) .
ts group had significantly higher scores of no adhesive remaining on the tooth in the bonding area ( score 0 ) then other groups .
direct bonding of orthodontic brackets using the acid- etching of tooth substrate has become a common technique in orthodontics8,14 .
moreover , there is a need for improving the capacity to obtain adequate bond strength , minimizing the amount of enamel loss9,17 and reducing the technique sensitivity of some adhesive systems .
an effective adhesion to the enamel depends on the acidic capacity to demineralize the enamel prisms , producing porosities and allowing a low viscosity resin to penetrate into them , resulting in micromechanical retention .
acid etching technique in " etch and rinse " systems requires rinsing and drying before application of the adhesive resin . while the self - etching primers are able to produce simultaneously prisms demineralization and adhesive infiltration10,16 eliminating the clinical rinsing step .
phosphoric acid etching has been blamed for decalcification and the development of white spot lesions around bonded orthodontic appliances 4,20 .
some reports have mentioned the mechanical damage to the enamel during debonding and removal of the remaining resin after acid etching11,12,15 .
some studies observed by field - emission electron scanning microscopy ( fe - sem ) more dissolution of the enamel surface as a result of phosphoric acid treatment than from self - etching primer procedures2,3 .
thus , reduction of enamel loss may be achieved using self - etching primer adhesives .
important issues should be pointed out during the choice of bonding materials for orthodontic brackets , such as their bond strength , longevity and ease of removal without damaging the dental structure .
those in vitro characteristics support the clinical practice through the shear bond strength and ari scores .
the correlation between in vitro and in vivo adhesive / resin interfaces and bond strength tests has been shown elsewhere24 .
this " in vitro " research evaluated the influence of saliva contamination on the bond strength of different adhesive systems used with light - cured composite for bonding orthodontic brackets to human enamel .
the results showed no significant difference in shear bond strength between contaminated and no contamination conditions for the same adhesive systems7,13 .
however , there are reports that demonstrate reduction of the bond strength when using self - etching primers3,12,17,22 . despite the difficult to compare absolute means results of different studies this
partially agree with our findings that transbond xt group ( t ) presented higher shear bond strength than the self - etching contaminated groups ( as and xs ) .
the contamination was done after the acid etching step in group ts and before the self - etching primer application in groups as and xs .
thus , saliva could buffer the acidic monomers reducing its capacity of etch the substrate decreasing the mean bond strength for this groups . even though , there was no significant difference between the not contaminated and contaminated conditions in the self - etching adhesive system groups .
restorative dentistry requires high bond strength and durability of adhesive procedures , while in orthodontics the bracket adhesion to tooth substrate is temporary .
when brackets debond , the failure at the bracket - adhesive interface or cohesively in adhesive is more desirable and safer than failure at the adhesive - enamel interface , since enamel fracture and crazing have been reported at the time of fracture 13 . the most frequent pattern of bonding failure in the self - etching groups occurred at the bracket - adhesive interface and none enamel fracture was observed .
moreover , saliva contamination did not influence the failure patterns of self - etching and the " etch and rinse " adhesive systems groups .
thus , it seems that self - etching primers does not represent a risk for irreversible damage to tooth substrate since few specimens presented remaining adhesive on enamel surface .
the present study used the same saliva application time for all groups ( 5 s ) .
a prolonged contamination time reduces the bond strength for self - etching adhesive systems23 probably because of the monomers dissolution and the well known buffering capacity of saliva .
other important fact of saliva contamination is the biofilm formation on etched enamel surface18 that reduces the interpenetration capacity of etch - and - rinse systems monomers .
others studies are necessary to evaluate the influence of different times of contamination at brackets bond strength . considering the longevity of bonding procedure
, there is evidence that bond strength decreases over time at the adhesive / tooth interface2 .
however , these monomers are hydrophilic and so , more susceptible to hydrolytic degradation longitudinally6 . in this study ,
saliva contamination showed influence at immediately shear bond strength of orthodontic brackets between " etch and rinse " and self - etch adhesive systems .
further studies are necessary to evaluate the longitudinal behavior of these adhesive systems on orthodontic brackets with or without saliva contamination as well as to support its application in the orthodontic treatment .
in conclusion , saliva contamination showed little influence on the immediate shear bond strength of orthodontic brackets .
 objective : to evaluate the influence of saliva contamination on shear bond strength and the bond failure pattern of 3 adhesive systems ( transbond xt , adhese and xeno iii ) on orthodontic metallic brackets bonded to human enamel.material and methods : seventy - two permanent human molars were cut longitudinally in a mesiodistal direction , producing seventy - two specimens randomly divided into six groups . 
 each system was tested under 2 different enamel conditions : no contamination and contaminated with saliva . in t , 
 a and x groups , the adhesive systems were applied to the enamel surface in accordance with manufacturer 's instructions . in ts , as and xs groups , saliva was applied to enamel surface followed by adhesive system application . 
 the samples were stored in distilled water at 37c for 24 h , and then tested for shear bond strength in a universal testing machine ( emic , dl 2000 ) running at a crosshead speed of 1 mm / min . 
 after bond failure , the enamel surfaces were observed under an optical microscope at 40x magnification.results:the control and contaminated groups showed no significant difference in shear bond strength for the same adhesive system . 
 however , shear bond strength of t group ( 17.034.91 ) was significantly higher than that of as ( 8.581.73 ) and xs ( 10.394.06 ) groups ( p<0.05 ) . 
 regarding the bond failure pattern , ts group had significantly higher scores of no adhesive remaining on the tooth in the bonding area than other groups considering the adhesive remnant index ( ari ) used to evaluate the amount of adhesive left on the enamel.conclusion:saliva contamination showed little influence on the 24-h shear bond strength of orthodontic brackets . 
INTRODUCTION
MATERIAL AND METHODS
RESULTS
DISCUSSION
CONCLUSIONS