the osteoporotic fractures in men ( mros ) study is a longitudinal observational study performed at six clinical sites in the u.s . for the primary aim of studying fracture determinants in older men . at baseline ,
5,994 community - dwelling ambulatory men aged 65 years were enrolled using mass mailing targeted to age - eligible men , as described previously ( 8,9 ) .
the protocol was approved by each study site institutional review board , and written informed consent was obtained from all participants .
the initial study visits occurred between march 2000 and april 2002 , and participants returned for a second clinic visit between march 2005 and may 2006 .
a total of 12% of participants died before the second clinic visit , 9.3% were alive but terminated the study before the second visit , and 1.5% responded to postcard questions instead of attending the second visit .
participants who did not participate in the second clinic visit were on average older and had poorer self - rated health and a higher prevalence of cardiac disease , diabetes , and hypertension than participants in our analytic cohort . compared with normoglycemic men ,
the age - adjusted risk of mortality did not differ significantly for men with ifg ( hazard ratio 0.99 , 95% ci 0.701.41 ) or for men with diabetes ( 1.44 , 0.982.12 ) .
the cohort in the present analysis comprised 3,752 participants with complete body composition measurements from dual - energy absorptiometry ( dxa ) at baseline and visit 2 and complete measures for fasting glucose , triglycerides , hdl cholesterol levels , systolic blood pressure , and medication use for diabetes , hypertension , and dyslipidemia at baseline .
weight was taken with balance beam or digital scales , and height was measured with wall - mounted stadiometers .
mean systolic blood pressure was calculated as the mean of blood pressure measured twice using a mercury sphygmomanometer .
questionnaires and interviews were used to obtain demographic , lifestyle , and health data including age , race ( white or other ) , education ( greater than or equal to a college degree versus less than a college degree ) , clinic site , alcohol use ( moderate / high defined as 7 vs. < 7 drinks / week ) , current smoking status , physical activity levels using the physical activity scale for the elderly ( pase ) score ( 10 ) , self - reported health status ( excellent / good vs. fair / poor / very poor ) , and medical conditions ( self - reported physician diagnosis of hypertension , diabetes , cancer , stroke , myocardial infarction , congestive heart failure , and angina ) .
participants were categorized as having hypertension if their mean systolic blood pressure was 140 mmhg or they reported a physician diagnosis of hypertension .
men were considered to have cardiac disease if they reported a physician diagnosis of a myocardial infarction , congestive heart failure , or angina .
the presence of dyslipidemia was characterized by hdl cholesterol < 40 mg / dl , triglycerides 200 mg / dl , or the use of one or more lipid - lowering medication(s ) .
participants were instructed to bring in all prescription medications taken in the past 30 days to their clinic visit , and study coordinators recorded the medications .
the iowa drug information service drug vocabulary ( college of pharmacy , university of iowa , iowa city , ia ) was used to identify ingredient(s ) in the medications , and these data were stored in an electronic medications inventory database ( san francisco coordinating center , san francisco , ca ) ( 11 ) .
glucose was measured using a hexokinase method from previously unthawed serum ( northwest lipid metabolism and diabetes research laboratories , seattle , wa ) .
the interassay cv for glucose based on blind duplicates was < 3% . thawed and refrozen serum from the baseline visit was assayed for triglyceride and hdl cholesterol levels using a roche cobas integra 800 automated analyzer ( roche diagnostics , indianapolis , in ) at the veteran s administration clinical laboratory in portland , oregon .
total lean , appendicular lean , and total fat mass were derived from whole - body dxa scans ( qdr 4500w ; hologic , bedford , ma ) at baseline and visit 2 .
the hologic reading provided values for total bone mineral content , fat mass , and fat - free mass for each whole - body dxa scan .
total lean mass was calculated by subtracting total whole - body bone mineral content from total fat - free mass . for all clinic sites ,
the cvs were < 1.7% for measurements of fat mass and < 1.5% for measurements of lean mass .
the dxa operators adjusted the cut lines on the whole - body dxa scans to define regions according to standard hologic procedures .
cut lines were drawn between the head of the humerus and the scapula at the glenoid fossa for delineation of the arm and across the midpoint of the femoral neck for delineation of the leg , and appendicular lean mass was the sum of lean mass in the arms and legs .
a hologic whole - body phantom was scanned three times weekly at each clinic site for the calculation and application of correction factors to adjust for longitudinal drift in dxa measures .
reproducibility was ensured by certifying dxa operators , using standardized scanning procedures and using a central quality control laboratory ( san francisco coordinating center ) .
categories of baseline glycemic status were created using fasting glucose levels , self - reported diagnosis of diabetes , and the medication inventory ( fig .
men without self - reported diabetes or use of diabetes medications were considered to have normoglycemia if their fasting glucose level was < 100 mg / dl and ifg if their fasting glucose level was 100125 mg / dl .
men with a fasting glucose 126 mg / dl and/or self - reported diabetes were considered to have undiagnosed / untreated diabetes if they did not use medications to treat diabetes and treated diabetes if they used medication(s ) to treat diabetes .
men with treated diabetes were further characterized as using insulin sensitizers ( metformin and/or thiazolidinediones ) at baseline or not , since studies using hyperinsulinemic - euglycemic clamps demonstrated improved peripheral insulin sensitivity with these medications ( 1215 ) .
additional diabetes medications taken by participants included insulin ( n = 42 ) , sulfonylureas ( n = 158 ) , and meglitinides ( n = 4 ) .
differences in baseline characteristics by glycemic and diabetes treatment categories were assessed using the  test for categorical variables and anova for continuous variables .
median absolute and percent changes in weight , total body fat mass , total body lean mass , and appendicular lean mass between baseline and visit 2 were calculated for these categories .
linear regression models were used to determine differences in percentage change in total lean and appendicular lean mass for men in glycemic and diabetes treatment categories ( ifg , untreated diabetes , diabetes treated with insulin sensitizers , and diabetes treated without insulin sensitizers ) using normoglycemic men as the referent group .
covariates that were significant in the baseline table and also significantly associated with the percentage change in total or appendicular lean mass ( p < 0.05 ) using anova for categorical variables or spearman correlation for continuous variables were considered confounders and were included in the multivariable regression model .
fasting glucose was not included in the multivariable model , since it was used to generate our glycemic and diabetes treatment categories .
because of collinearity with other baseline body composition measurements , bmi was not included in the multivariable model .
postestimation comparisons using the wald test were performed to compare changes in lean mass for diabetic men on insulin sensitizers with untreated diabetes and diabetes treated without insulin sensitizers .
adjusted least - squares mean ( lsm ) percentage changes in total lean and appendicular lean mass for each category were calculated from the multivariable models .
these analyses were repeated for glycemic and diabetes treatment categories restricted to participants who remained in consistent categories of normoglycemia , ifg , untreated diabetes , diabetes treated with insulin sensitizers , and diabetes treated without insulin sensitizers at the follow - up visit .
statistical analyses were performed using stata / ic 11.0 ( statacorp lp , college station , tx ) .
the osteoporotic fractures in men ( mros ) study is a longitudinal observational study performed at six clinical sites in the u.s . for the primary aim of studying fracture determinants in older men . at baseline ,
5,994 community - dwelling ambulatory men aged 65 years were enrolled using mass mailing targeted to age - eligible men , as described previously ( 8,9 ) .
the protocol was approved by each study site institutional review board , and written informed consent was obtained from all participants .
the initial study visits occurred between march 2000 and april 2002 , and participants returned for a second clinic visit between march 2005 and may 2006 .
a total of 12% of participants died before the second clinic visit , 9.3% were alive but terminated the study before the second visit , and 1.5% responded to postcard questions instead of attending the second visit .
participants who did not participate in the second clinic visit were on average older and had poorer self - rated health and a higher prevalence of cardiac disease , diabetes , and hypertension than participants in our analytic cohort . compared with normoglycemic men ,
the age - adjusted risk of mortality did not differ significantly for men with ifg ( hazard ratio 0.99 , 95% ci 0.701.41 ) or for men with diabetes ( 1.44 , 0.982.12 ) .
the cohort in the present analysis comprised 3,752 participants with complete body composition measurements from dual - energy absorptiometry ( dxa ) at baseline and visit 2 and complete measures for fasting glucose , triglycerides , hdl cholesterol levels , systolic blood pressure , and medication use for diabetes , hypertension , and dyslipidemia at baseline .
weight was taken with balance beam or digital scales , and height was measured with wall - mounted stadiometers .
mean systolic blood pressure was calculated as the mean of blood pressure measured twice using a mercury sphygmomanometer .
questionnaires and interviews were used to obtain demographic , lifestyle , and health data including age , race ( white or other ) , education ( greater than or equal to a college degree versus less than a college degree ) , clinic site , alcohol use ( moderate / high defined as 7 vs. < 7 drinks / week ) , current smoking status , physical activity levels using the physical activity scale for the elderly ( pase ) score ( 10 ) , self - reported health status ( excellent / good vs. fair / poor / very poor ) , and medical conditions ( self - reported physician diagnosis of hypertension , diabetes , cancer , stroke , myocardial infarction , congestive heart failure , and angina ) .
participants were categorized as having hypertension if their mean systolic blood pressure was 140 mmhg or they reported a physician diagnosis of hypertension .
men were considered to have cardiac disease if they reported a physician diagnosis of a myocardial infarction , congestive heart failure , or angina .
the presence of dyslipidemia was characterized by hdl cholesterol < 40 mg / dl , triglycerides 200 mg / dl , or the use of one or more lipid - lowering medication(s ) .
participants were instructed to bring in all prescription medications taken in the past 30 days to their clinic visit , and study coordinators recorded the medications . the iowa drug information service drug vocabulary ( college of pharmacy , university of iowa , iowa city , ia ) was used to identify ingredient(s ) in the medications , and these data were stored in an electronic medications inventory database ( san francisco coordinating center , san francisco , ca ) ( 11 ) .
glucose was measured using a hexokinase method from previously unthawed serum ( northwest lipid metabolism and diabetes research laboratories , seattle , wa ) .
the interassay cv for glucose based on blind duplicates was < 3% . thawed and refrozen serum from the baseline visit was assayed for triglyceride and hdl cholesterol levels using a roche cobas integra 800 automated analyzer ( roche diagnostics , indianapolis , in ) at the veteran s administration clinical laboratory in portland , oregon .
total lean , appendicular lean , and total fat mass were derived from whole - body dxa scans ( qdr 4500w ; hologic , bedford , ma ) at baseline and visit 2 .
the hologic reading provided values for total bone mineral content , fat mass , and fat - free mass for each whole - body dxa scan .
total lean mass was calculated by subtracting total whole - body bone mineral content from total fat - free mass . for all clinic sites ,
the cvs were < 1.7% for measurements of fat mass and < 1.5% for measurements of lean mass .
the dxa operators adjusted the cut lines on the whole - body dxa scans to define regions according to standard hologic procedures .
cut lines were drawn between the head of the humerus and the scapula at the glenoid fossa for delineation of the arm and across the midpoint of the femoral neck for delineation of the leg , and appendicular lean mass was the sum of lean mass in the arms and legs .
a hologic whole - body phantom was scanned three times weekly at each clinic site for the calculation and application of correction factors to adjust for longitudinal drift in dxa measures .
reproducibility was ensured by certifying dxa operators , using standardized scanning procedures and using a central quality control laboratory ( san francisco coordinating center ) .
categories of baseline glycemic status were created using fasting glucose levels , self - reported diagnosis of diabetes , and the medication inventory ( fig .
1 ) . men without self - reported diabetes or use of diabetes medications were considered to have normoglycemia if their fasting glucose level was < 100 mg / dl and ifg if their fasting glucose level was 100125 mg / dl .
men with a fasting glucose 126 mg / dl and/or self - reported diabetes were considered to have undiagnosed / untreated diabetes if they did not use medications to treat diabetes and treated diabetes if they used medication(s ) to treat diabetes .
men with treated diabetes were further characterized as using insulin sensitizers ( metformin and/or thiazolidinediones ) at baseline or not , since studies using hyperinsulinemic - euglycemic clamps demonstrated improved peripheral insulin sensitivity with these medications ( 1215 ) .
additional diabetes medications taken by participants included insulin ( n = 42 ) , sulfonylureas ( n = 158 ) , and meglitinides ( n = 4 ) .
differences in baseline characteristics by glycemic and diabetes treatment categories were assessed using the  test for categorical variables and anova for continuous variables .
median absolute and percent changes in weight , total body fat mass , total body lean mass , and appendicular lean mass between baseline and visit 2 were calculated for these categories .
linear regression models were used to determine differences in percentage change in total lean and appendicular lean mass for men in glycemic and diabetes treatment categories ( ifg , untreated diabetes , diabetes treated with insulin sensitizers , and diabetes treated without insulin sensitizers ) using normoglycemic men as the referent group .
covariates that were significant in the baseline table and also significantly associated with the percentage change in total or appendicular lean mass ( p < 0.05 ) using anova for categorical variables or spearman correlation for continuous variables were considered confounders and were included in the multivariable regression model .
fasting glucose was not included in the multivariable model , since it was used to generate our glycemic and diabetes treatment categories .
because of collinearity with other baseline body composition measurements , bmi was not included in the multivariable model .
postestimation comparisons using the wald test were performed to compare changes in lean mass for diabetic men on insulin sensitizers with untreated diabetes and diabetes treated without insulin sensitizers .
adjusted least - squares mean ( lsm ) percentage changes in total lean and appendicular lean mass for each category were calculated from the multivariable models .
these analyses were repeated for glycemic and diabetes treatment categories restricted to participants who remained in consistent categories of normoglycemia , ifg , untreated diabetes , diabetes treated with insulin sensitizers , and diabetes treated without insulin sensitizers at the follow - up visit .
statistical analyses were performed using stata / ic 11.0 ( statacorp lp , college station , tx ) .
at baseline , this cohort of 3,752 men had a mean age of 72.7 years ( range 6592 years ) and a bmi of 27.4 kg / m . when separated into glycemic categories , 1,853 men had normoglycemia , 1,403 had ifg , 234 had untreated diabetes , 151 had diabetes treated with insulin sensitizers , and 111 had diabetes treated without insulin sensitizers .
these groups differed significantly at baseline by race , education , alcohol use , self - rated health , cardiovascular disease , hypertension , dyslipidemia , -blocker and ace inhibitor use , and total body lean and fat mass ( table 1 ) .
baseline characteristics by glycemic and diabetes treatment categories data are proportions or means ( sd ) .
p values are provided for anova or  test . over a mean duration of 3.5  0.7 years of follow - up
, men lost on average 1.5% in total body weight , gained 1.6% total body fat , lost 2.0% total body lean mass , and lost 3.2% appendicular lean mass .
men with untreated diabetes or diabetes treated without insulin sensitizers had the greatest median absolute and percentage loss in weight and total fat , total lean , and appendicular lean mass ( table 2 ) . men who were taking insulin sensitizers to treat diabetes
median ( interquartile range ) absolute and percent change (  ) in body weight , total fat , total lean , and appendicular lean mass between visits 1 and 2 by glycemic and diabetes treatment categories over 3.5 years in linear regression models adjusted for age , race , and clinic site , individuals with ifg , untreated diabetes , or diabetes treated without insulin sensitizers had a greater percentage loss in total or appendicular lean mass than normoglycemic men ( table 3 ) . in models further adjusted for medical comorbidities and physical and lifestyle characteristics , the percentage loss in appendicular lean mass in these categories still exceeded that of normoglycemic men .
in contrast , the percentage of total or appendicular lean mass lost in men with diabetes treated with insulin sensitizers was significantly less than that of normoglycemic men in both models .
when compared with men who had untreated diabetes or diabetes treated without insulin sensitizers , men with diabetes treated with insulin sensitizers also had a lower percentage loss in total lean mass ( p < 0.001 ) and appendicular lean mass ( p < 0.001 ) for these wald test comparisons using model 1 .
although the frequency of meglitinide use did not differ significantly between groups of insulin sensitizer treatment , there was a higher frequency of insulin and sulfonylurea use among men with diabetes who were not taking insulin sensitizers .
neither adjustment for sulfonylurea nor insulin use in these multivariate models altered our results significantly ( results not shown ) .
adjusted lsm percentage change ( 95% ci ) in total lean and appendicular lean mass by glycemic and diabetes treatment categories over 3.5 years model 1 is adjusted for age , race , and clinic site .
model 2 includes model 1 covariates + self - rated health , cardiac disease , hypertension , dyslipidemia , education , pase , baseline total fat mass , and total lean mass . *
additional investigation of the lean mass changes associated with specific insulin sensitizers was performed as post hoc analyses in models adjusted for age , race , and clinic site .
men with diabetes treated with thiazolidinediones ( n = 31 ) did not differ significantly from normoglycemic men in the amount of total lean mass loss (  = 0.62 , p = 0.37 , lsm change 2.4% [ 95% ci 3.7 to 1.0 ] ) or appendicular lean mass loss (  = 0.15 , p = 0.86 , lsm change 3.1% [ 4.8 to 1.4 ] ) . although men with diabetes treated with metformin ( n = 104 ) lost less total lean ( lsm change 1.5% [ 2.2 to 0.7 ] ) and appendicular lean mass ( lsm change 2.4% [ 3.4 to 1.5 ] ) than normoglycemic men , the differences were not statistically significant ( total lean :  = 0.27 , p = 0.49 , and appendicular lean :  = 0.49 , p = 0.32 ) .
men with diabetes treated with metformin + thiazolidinedione ( n = 16 ) had a significant gain in total lean mass (  = 2.59 , p < 0.01 , lsm change + 0.8% [ 1.0 to 2.7 ] ) and appendicular lean mass (  = 3.44 , p < 0.01 , lsm change = + 0.5% [ 1.8 to 2.9 ] ) compared with normoglycemic men .
men treated with metformin or metformin + thiazolidinedione had significantly less total or appendicular lean mass percentage loss than men with untreated diabetes ( p < 0.005 ) or men with diabetes treated without insulin sensitizers ( p < 0.001 ) for these wald test comparisons in age- , race- , and clinic site  adjusted models . when the above analyses were repeated after restricting to men remaining in consistent glycemic and diabetes treatment categories at visit 2 , all of the results above remained unchanged .
however , while the percentage loss in appendicular lean mass for men with diabetes treated with metformin + thiazolidinedione ( lsm change
0.2% [ 95% ci 3.0 to 2.6 ] ) was still less than for normoglycemic men ( lsm change 2.9%
[ 3.1 to 2.7 ] ) , the findings were no longer significantly different ( metformin + thiazolidinedione :  = 2.72 , p = 0.06 ) .
our study confirms the greater loss in total and appendicular lean mass in older men with untreated diabetes compared with normoglycemic men that has been described in a slightly older cohort of men and women ( 4 ) .
the study also provides additional evidence of greater total and appendicular lean mass loss in older men with ifg or with diabetes treated without insulin sensitizers compared with normoglycemic men .
however , the loss in total or appendicular lean mass for men who had diabetes treated with insulin sensitizers was significantly less than that for normoglycemic men .
furthermore , men with diabetes using insulin sensitizers lost significantly less total or appendicular lean mass than men with untreated diabetes or diabetes treated without insulin sensitizers .
these data show that conditions with underlying insulin resistance are associated with greater muscle loss and suggest that such loss may be prevented with pharmacological treatment of insulin resistance .
metformin and thiazolidinediones are among the most frequent therapies used to treat type 2 diabetes , and both drugs can improve insulin sensitivity ( 12,13 ) . because greater insulin resistance is associated with greater losses in total and appendicular lean mass ( 6 )
, one could hypothesize that the reduction of insulin resistance with insulin sensitizer treatment in men with type 2 diabetes would attenuate this loss .
indeed , our findings revealed that diabetic men treated with insulin sensitizers had significantly less appendicular lean mass loss than diabetic men who were untreated or using other therapies .
whereas it is possible that glycemic control differs between these categories , it seems unlikely that these findings are explained by differences in dysglycemia , since the loss in lean mass was less for men with diabetes treated with insulin sensitizers and more for men with diabetes treated without insulin sensitizers when compared with normoglycemic men , despite higher levels of fasting glucose in men with diabetes .
further analysis of specific insulin sensitizers revealed that diabetic men on metformin or metformin + thiazolidinediones had significantly less total and appendicular lean mass loss than men with untreated diabetes or diabetes treated without insulin sensitizers .
it is possible that additional differences for men taking thiazolidinediones alone were not detected because of the small sample size of this group .
in contrast to sulfonylureas that act on the pancreas to stimulate insulin secretion , insulin sensitizers can act peripherally on muscle , similar to the effects of exercise on muscle , to activate 5-adenosine monophosphate  activated protein kinase ( ampk ) and stimulate gene expression of peroxisome proliferator  activated receptor- coactivator 1- ( pgc1 ) to upregulate the transcription of genes to enhance fatty acid oxidation for reduced muscle lipid accumulation , stimulate angiogenesis , increase mitochondrial biogenesis , and switch muscle fiber types from glycolytic to more oxidative fatigue - resistant fibers ( 1620 ) .
further evidence from basic studies demonstrates that muscle - specific overexpression of pgc1 in transgenic mice increased muscle mass and reduced the number of falls ( 21 ) .
in addition , treatment of mice with aminoimidazole carboxamide ribonucleotide ( aicar ) , an ampk agonist , increased phosphorylation of ampk in the quadriceps muscle , induced expression of pgc1 in myoblasts , improved endurance , and extended running distance ( 22 ) .
more translational studies are needed to further understand the effects of insulin sensitizers on muscle in humans .
this is a cohort of older predominantly white men who were ambulatory and healthy enough to participate in follow - up visits ; therefore , our results may not be generalized to ailing men , minorities , older women , or younger populations . because this is an observational study without randomization to different diabetes treatments , confounding by indication can not be excluded .
although adjustment for between - group differences did not materially change the significant associations among diabetes treatment groups , residual confounding may remain .
data on treatment adherence were not available , but results were similar when information derived from patient report was used to restrict analyses to men consistently reporting diabetes diagnosis and medication use at visit 2 .
misclassification of men with untreated diabetes was possible , since oral glucose tolerance testing was not performed in the mros study ; therefore , the loss in lean mass in men with ifg may be due to inclusion of men with untreated diabetes .
it is possible that some men who were categorized as having treated diabetes had insulin - sensitive type 1 diabetes rather than insulin - resistant type 2 diabetes .
however , analyses repeated excluding the 42 participants on insulin did not materially change our results ( data not shown ) . in summary ,
muscle loss was accelerated in individuals with ifg , untreated diabetes , or diabetes treated without insulin sensitizer medications . however , the loss in muscle mass was markedly attenuated in diabetic men using insulin sensitizers .
these results suggest that insulin resistance may be causally linked to muscle loss in age - related sarcopenia .
furthermore , they raise the need for randomized clinical trials of insulin sensitizers to understand their potential effects on muscle loss and possible preventative or therapeutic applications in sarcopenia .
 objectiveto examine longitudinal changes in total and appendicular lean body mass in older men with impaired fasting glucose ( ifg ) or diabetes and to determine whether these changes differ by diabetes treatment.research design and methodsa total of 3,752 ambulatory men aged 65 years at baseline participated in a multicenter longitudinal cohort study . 
 baseline glycemic status was categorized as normoglycemia , ifg , undiagnosed / untreated diabetes , or treated diabetes . 
 insulin sensitizer medication use ( metformin and/or thiazolidinediones ) was assessed by prescription medication inventory . 
 the change in total lean and appendicular lean mass was derived from dual x - ray absorptiometry scans taken at baseline and 3.5  0.7 years later.resultsthis male cohort included 1,853 individuals with normoglycemia , 1,403 with ifg , 234 with untreated diabetes , 151 with diabetes treated with insulin sensitizers , and 111 with diabetes treated without insulin sensitizers . 
 men with untreated diabetes , diabetes treated without insulin sensitizers , or ifg had greater percentage loss in total or appendicular lean mass ( p  0.05 in comparison to normoglycemic men ) . there remained a significantly greater percentage loss in appendicular lean mass for these groups even after adjustment for medical comorbidities or lifestyle factors . 
 in contrast , the percentage loss in total or appendicular lean mass in men with diabetes treated with insulin sensitizers was significantly less than that in normoglycemic men in minimally and fully adjusted models.conclusionsskeletal muscle loss was accelerated in men with ifg and diabetes , except when the latter was treated with insulin sensitizers . 
 these findings suggest that insulin sensitizers may attenuate muscle loss . 
RESEARCH DESIGN AND METHODS
Study population and design
Baseline study visit measurements
Biochemical measurements
Body composition measurements
Glycemic status
Statistical analysis
RESULTS
CONCLUSIONS