classical fear conditioning provides one of the most powerful models for studying the neural mechanisms of fear and pathological anxiety , both in animals and humans ( mahan and ressler , 2012 ) . during fear conditioning
, associations are formed between an aversive stimulus and conditioned stimuli such as neutral environmental cues that are on the whole characteristic for that particular aversive situation .
re - exposure to the conditioned stimulus in absence of the predicted punishment eventually leads to fear extinction .
typically a fear response spontaneously recovers with the passage of time indicating that the original fear memory was not erased but complemented by a parallel inhibitory memory trace encoding the safety of the conditioned stimulus in that particular extinction context ( myers and davis , 2007 ) .
extinction of conditioned fear represents the basic paradigm of current exposure - based interventions in the therapy of human anxiety disorders ( phelps et  al . , 2004 ) .
the formation of context - specific fear extinction is believed to involve a distributed neuronal network comprising the hippocampus , thalamus and ventromedial prefrontal cortex ( mpfc ) ( quirk and mueller , 2008 ) , which are strongly interconnected with the amygdaloid complex ( pitknen , 2000 ) .
the role of the mpfc was proposed to ultimately control amygdala activity ( quirk and beer , 2006 ) .
in fact , stimulation of the infralimbic component ( il ) of the mpfc blocks the expression of conditioned fear , likely by altering inhibitory networks within the amygdala ( amano et  al . , 2010 ) . over the past decade evidence
has accumulated pointing to a critical role of the basolateral complex of the amygdala ( bla ) in the expression of contextual fear ( goosens and maren , 2001 ; muller et  al . , 1997 ) and in the acquisition of extinction ( herry et  al . , 2010
while enhanced activation of inhibitory circuits within the bla may constrain the impact of sensory inputs at principal neurons , the intercalated cell clusters ( itcs ) have been proposed to act as an inhibitory gate between the bla and the central nucleus under cortical control , thus regulating amygdala output ( ehrlich et  al . , 2009 ; pape and pare , 2010 ) .
itcs have emerged as a pivotal structure for inhibition of the expression of conditioned fear and for the storage of extinction memory ( likhtik et  al . , 2008 ) .
although in recent years the cellular organization of the itcs has been intensively investigated ( busti et  al . , 2011 ; geracitano et  al . , 2007 ; manko et  al . , 2011
; royer et  al . , 2000 ) , many of the neural circuits and neurochemical systems involving the itcs remain unclear .
anatomical evidence has shown that group iii metabotropic glutamate ( mglu ) receptors are enriched in the amygdala and particularly in association with the itcs ( masugi et  al . , 1999 ) .
selective activation or blockade of these mglu receptors was shown to alter anxiety and fear responses ( swanson et  al . , 2005 ) .
in particular , mglu7-null mice showed a marked reduction in the acquisition of appetitive and aversive conditioning ( callaerts - vegh et  al .
, 2006 ; masugi et  al . , 1999 ) , as well as deficits in the extinction of conditioned emotional responses ( fendt et  al . , 2008 ;
measures of anxiety in mglu8-deficient mice gave rise to inconsistent results ( duvoisin et  al .
, 2002 ; robbins et  al . , 2007 ) . however , recent work revealed in these animals robust deficits in the expression of contextual conditioned fear and chlordiazepoxide - induced facilitation of extinction ( fendt et  al . , 2010 ) . here , we investigated the potential influence of acute pharmacological activation of mglu7 and mglu8 receptors on extinction of contextual fear .
in addition , we carried out a detailed characterization of the synaptic distribution of these receptors in the neural circuits within the amygdala likely implicated in the extinction of fear conditioning .
large neurons present at the border of itc clusters and expressing the mglu1 receptor ( busti et  al . , 2011 )
the reported studies were carried out on adult male c57bl/6j mice ( 2530  g ) . before use , the animals were housed in groups of five under controlled laboratory conditions ( 12:12  h light / dark cycle with lights on at 07:00  h ; 21    1  c ; 60% humidity ) with food and water ad libitum for at least two weeks after delivery from the supplier ( institut fr labortierkunde und genetik , university of vienna , himberg , austria or japan slc inc . ,
all experimental protocols were approved by the austrian animal experimentation ethics board ( gz66.011/58-brgt/2004 and gz66.011/02-brgt/2007 ) or by the national institute for physiological science 's animal care and use committee in compliance with both the european convention for the protection of vertebrate animals used for experimental and other scientific purposes ( ets no .
123 ) and the european communities council directive of 24 november 1986 ( 86/609/eec ) .
the authors further attest that all efforts were made to minimize animal suffering , the number of animals used , and to use alternatives to in  vivo techniques whenever available .
guide canulae ( 25 gauge , 11  mm in length ) , aimed to target the dorsal pole of the bla [ 1.2  mm caudal , 3.2  mm lateral and 4.0  mm ventral to bregma according to the franklin and paxinos mouse brain atlas ( 2001 ) ] , were bilaterally implanted into the skull of anaesthetised mice ( n  =  51 ; 50  mg / kg i.p .
seven days after surgery and 15  min before the onset of the behavioural experiments , the stylets of the guide canulae were replaced by injection microcanulae ( 31 gauge ) , which extended the guide canulae by 1  mm , thus , reaching the bla .
( s)-3,4-dcpg ( tocris , bristol , uk ) and amn082 ( synthesized internally at novartis ) were dissolved in artificial cerebrospinal fluid ( acsf ) .
mice were randomly assigned to 3 treatment groups , which were injected with either vehicle ( acsf ) , amn082 ( 300  pmol ) or ( s)-3,4-dcpg ( 10  nmol ) in a volume of 0.1  l per side at a rate of 0.05  l / min .
behavioural experiments were performed between 09:00 and 16:00  h. fear acquisition , extinction training and extinction recall were performed in two identical , brightly illuminated ( 300 lux ) conditioning chambers ( 26    30    32  cm ; coulbourn instruments , usa ) with floors of stainless steel bars .
the rods were connected to a shock source and grid scrambler ( coulbourn instruments , usa ) for delivery of the footshock used as unconditioned stimulus .
video cameras were mounted above each chamber for recording of the animal 's behaviour during the exposure to the context . for fear acquisition , mice were placed into the conditioning chamber and five mild foot shocks ( 0.7  ma , 2  s ) were delivered . a 2  min stimulus - free period preceded , separated and followed the unconditioned stimulus presentations .
after 24 and 48  h , animals were replaced into the same chamber for 30  min of extinction training and 8  min of extinction recall in the absence of unconditioned stimulus presentations , respectively .
each testing session was continuously recorded and the freezing behaviour displayed by the mice was subsequently analyzed in 2  min bin intervals for fear acquisition , in 1  min bin intervals for fear extinction or for the total time interval for extinction recall , by an experienced observer blind to the treatment groups .
the simultaneous display of a horizontal position of the head , rigid hunched position and stiffening / lifting of the tail by the mice was taken as freezing behaviour ( fanselow , 1980 ) .
unconditioned mice were placed into the conditioning chamber and their spontaneous activity was recorded for 30  min by a video camera positioned above the chamber .
the distance travelled was registered by a tracking system ( videomot2 , tse systems gmbh , bad homburg , germany ) . in addition , the time each mouse spent in freezing behaviour was analyzed as described above .
possible effects of ( s)-3,4-dcpg on motor coordination were tested on the accelerating rotarod ( tse systems gmbh , bad homburg , germany ) according to a previous protocol with slight modifications ( singewald et  al . , 2004 ) .
mice were placed onto a slowly ( 4  rpm ) rotating rod , which was then continuously accelerated to 40  rpm within 5  min .
mice were given an intense training ( 3    3 with a 5  min intertrial interval ) on the first day and further training sessions ( 1    3/day ) on the two following days .
on day 4 , mice were injected with either ( s)-3,4-dcpg or acsf and given two test trials 20 and 40  min later , which corresponded to time points 5 and 25  min spent in the conditioning chambers .
the latency to fall off the rod was automatically recorded . the maximum time on the rotarod
was set at 5  min . at the end of each behavioural experiment , animals were killed by an overdose of pentobarbital and the exact injection sites were histologically established in 50  m - thick brain sections stained with cresyl violet .
behavioural data were analyzed only from those animals that were injected at the correct target sites .
for immunocytochemical light and electron microscopy experiments , mice were deeply anesthetized by intraperitoneal injection of thiopental ( 250  mg / kg , i.p .
; sandoz , kundl , austria ) and perfused transcardially with phosphate buffered saline ( pbs ; 0.9% nacl , ph 7.4 ) followed by ice - cold fixative made of 4% w / v paraformaldehyde and 15% v / v of a saturated solution of picric acid in phosphate buffer ( pb ; 0.1  m , ph 7.4 ) for 15  min .
for electron microscopy experiments , glutaraldehyde was added to the fixative at a final dilution of 0.05% v / v just before the perfusion .
brains were immediately removed from the skull , washed in 0.1  m  pb and sliced coronally in 40 ( for light microscopy ) or 50  m ( for electron microscopy ) thick sections , unless otherwise specified , on a leica vt1000s vibratome ( leica microsystems , vienna , austria ) .
sections were stored in 0.1  m  pb containing 0.05% nan3 at 6  c until immunocytochemical experiments were performed .
for this study , 3 different polyclonal antibodies against mglu1 have been used , two of them raised in rabbit and one in guinea pig .
one of the rabbit polyclonal antibodies against mglu1 was raised to a bacterial fusion protein containing amino acid residues 8591199 of rat mglu1 ( shigemoto et  al . , 1997 ) .
immunoblot results indicated that the antibodies do not react with other mglu receptors or proteins ( shigemoto et  al . , 1997 ) .
the second rabbit polyclonal antibody against mglu1 was raised against a synthetic peptide corresponding to the amino acids 11161130 of the rat mglu1 sequence ( diasorin , stillwater , mn ) .
immunolabelling for this antibody was completely abolished by pre - adsorption with synthetic rat mglu1 ( manufacturer 's technical information ) and in brain sections of mice lacking the mglu1 receptor ( ferraguti et  al . , 2004 ) .
anatomy , hokkaido university , sapporo , japan ) and was raised against a fusion protein containing amino acid residues 9451127 of mouse mglu1 ( tanaka et  al . , 2000 ) .
also for this antibody immunolabelling was completely abolished in brain sections of mice lacking the mglu1 receptor ( ferraguti et  al . , 2004 ) .
the rabbit polyclonal anti - mglu7a antibody was raised against a bacterial fusion protein containing the amino acid residues 874915 of rat mglu7a ( shigemoto et  al .
immunoblot results and tests in heterologous expression systems indicated that the antibody does not react with other mglu receptors or proteins .
no immunolabelling was obtained in the brain of mice deficient in mglu7 protein ( sansig et  al . , 2001 ) .
the guinea pig polyclonal anti - mglu8a antibody was raised against a synthetic peptide corresponding to the last 23 amino acids of mouse mglu8a ( kinoshita et  al . , 1996 ) .
immunoblot analysis showed an immunoreactive band of the expected molecular weight , which disappeared after pre - adsorption of the antibody with the immunogenic peptide ( kinoshita et  al . , 1996 ) .
no immunolabelling was obtained in the brain of mice deficient in mglu8 protein ( ferraguti et  al . , 2005 ) .
guinea pig antibodies to the vesicular -aminobutyric acid transporter ( vgat ) , vesicular glutamate transporters ( vglut ) 1 and 2 were generous gifts from prof .
m. watanabe ( hokkaido university ) . biotinylated goat anti - pha - l was purchased from vector laboratories ( burlingame , ca ; product no .
immunofluorescent experiments were carried out according to previously published procedures ( ferraguti et  al . , 2004 ) using slices obtained from 4 mice .
briefly , free - floating sections were incubated in blocking solution , composed of 20% normal goat serum ( ngs ) , 0.1% tritonx ( tx ) , 0.9% nacl in 50  mm tris ( ph 7.4 ) for 1  h at room temperature ( rt ) and then in primary antibodies ( see table  1 for complete listing and dilutions ) , in combination or alone , made up in tbs , 0.1% tx , and 1% ngs for approximately 48  h ( 6  c ) .
subsequently , after extensive washes in tbs , sections were incubated overnight ( 6  c ) with a mixture of appropriate secondary antibodies ( see table  1 ) .
sections were then washed and mounted onto gelatin - coated slides in vectashield ( vector laboratories , burlingame , ca ) .
to control for a possible cross - reactivity between iggs in double and triple immunolabeling experiments , some sections were processed through the same immunocytochemical sequence , except that only one primary antibody was applied ; but the full complement of secondary antibodies was maintained .
in addition , the secondary antibodies utilized were highly pre - adsorbed to the iggs of numerous species .
immunofluorescence was studied using a zeiss axioimager m1 microscope ( zeiss , vienna , austria ) with epifluorescence illumination equipped with the following filter blocks : cy2/alexa488 ( excitation filter band pass [ bp ] 480/40  nm ; reflection short - pass filter 505  nm ; emission filter bp 527/30  nm ) and cy3 ( excitation filter bp 545/25  nm ; reflection short - pass filter 570  nm ; emission filter bp 605/70  nm ) .
images were displayed and analyzed by the openlab software ( version 5.5.0 ; improvision ) .
images were acquired through an orca - er ccd camera ( hamamatsu , hamamatsu city , japan ) .
brightness and contrast were adjusted for the whole frame with no part of the frame modified independently in any way .
for labelling of projections from the intralaminar thalamus and mpfc to the bla and itcs , as well as from bla to itcs , adult male mice ( n  =  10 ) were injected unilaterally with phaseolus vulgaris leucoagglutinin ( pha - l ) into these regions using previously published procedures ( gu and simerly , 1997 ) .
briefly , animals were anesthetized with 7% chloral hydrate in 0.9% nacl ( 0.5  ml/100  g body weight ) . a 2.5% solution of pha - l ( vector ) in 0.1  m sodium phosphate buffer ( ph 7.8 )
was delivered iontophoretically into the posterior amygdala ( bregma 1.6 ,  + 3.3  ml , 5.1 dv ) , intralaminar thalamus ( bregma 1.0 , 0  ml , 3.9 dv ) or il - mpfc ( bregma  + 2.0 ,  + 0.2  ml , 2.5 dv ) through a stereotaxically positioned glass micropipette ( tip diameter 1520  m ) , by applying a positive current ( 3.55  a , 7  s on / off intervals ) for 1030  min .
after 514 day survival period , mice were deeply anesthetized with sodium pentobarbital ( 50  mg / kg , i.p . ) and perfused transcardially as described in 2.5 . serial coronal sections
( 50  m thick ) , from injection sites to projection sites , were cut with a vibratome ( leica , vienna , austria ) .
every fifth section was used for immunocytochemical detection of pha - l for light microscopy .
sections were first incubated in blocking buffer composed of 2% ngs in pbs for 1  h and then overnight at rt with 5  g / ml biotinylated
anti - pha - l ( vector ) in pbs containing 0.3% tx and 1% ngs .
after extensive washes , the sections were incubated in the abc complex ( 1:100 , vector ) made up in pbs containing 0.1% tx for 2  h followed by diaminobenzidine ( dab ) ( 0.5  mg / ml ) and 0.003% h2o2 , as the electron donor , for 56  min . if the injection sites were correctly placed and projection sites were strongly labelled , amygdala sections from these animals were processed for pre - embedding electron microscopy .
pre - embedding immunocytochemistry experiments were carried out according to previously published procedures ( sreepathi and ferraguti , 2012 ) .
briefly , free - floating sections were washed three times in 0.1  m  pb , cryoprotected in 20% sucrose made in 0.1  m  pb overnight at 6  c .
after removal of the sucrose , the sections were freeze - thawed twice to allow antibody penetration and then incubated in 20% ngs in tbs for 2  h at rt .
after blocking , sections were exposed for 72  h at 6  c to primary antibodies ( see table  1 ) made up in a solution containing 2% ngs in tbs .
after three washes in tbs , sections were incubated overnight at 6  c with the appropriate secondary antibodies ( table  1 ) .
when a single primary antibody was used , it was visualized either by horseradish peroxidase ( hrp ) or by nanogold - silver - enhanced reaction .
when more than one primary antibody was used , one of them was visualized by nanogold - silver - enhanced reaction and the second was visualized by hrp reaction .
after several washes with tbs , sections incubated with fab fragment secondary antibodies coupled to nanogold ( 1.4  nm ) were extensively washed in milliq water , followed by silver enhancement of the gold particles with the hq kit ( nanoprobes inc . , stony brook , ny , usa ) for 510  min .
sections processed for the hrp reaction were incubated in abc complex ( diluted 1:100 ; vector ) made up in tb either overnight at 6  c or at rt for 2  h and then washed in tb several times before the antigen / antibody complex was visualized by means of the dab - h2o2 reaction .
sections were subsequently washed with 0.1  m  pb and treated with 2% oso4 in 0.1  m  pb for 30  min at rt .
after several washes with 0.1  m  pb and then with milliq water , sections were contrasted with 1% uranyl - acetate in 50% ethanol for 30  min at rt , making sure they were protected from light .
sections were washed with milliq water followed by graded ethanol and propylene oxide at rt .
sections were then quickly transferred into weighting boats containing epoxy resin ( durcupan acm - fluka , sigma , gillingham , uk ) and kept overnight at rt .
the following day , the sections were transferred onto siliconized slides , coverslipped with aclar  film coverslips ( ted pella , inc . , redding , ca ) , and incubated for 3 days at 60  c .
blocks containing specific nuclei of the amygdala were cut under a stereomicroscope and re - embedded in epoxy resin .
ultrathin sections ( 70  nm ) were cut using a diamond knife ( diatome , biel , switzerland ) on an ultramicrotome ( ultracut , leica , vienna , austria ) , collected on copper slot grids coated with pioloform ( agar , stansted , england ) and analyzed with a transmission electron microscope ( jeol jem-1010 or philips cm120 ) .
behavioural data are presented as mean    s.e.m . during 1 , 2 and 5  min time periods in contextual fear conditioning and locomotor activity studies , respectively .
statistical analysis of behavioural data was carried out by one - way anova or two - way anova with repeated measures followed by a fisher 's lsd test after a significant omnibus value .
p - levels < 0.05 were considered statistically significant , while p  <  0.08 was used to indicate a trend towards significance . to determine the sample size required for establishing the lack of group iii mglus in specific inputs we used prospectively power analysis .
sample size was obtained by testing for one proportion using the software java applets for power and sample size ( lenth , 2006 - 9 ) .
we assumed the frequency of pha - l positive axon terminals labelled for presynaptic mglu receptors to be 20% and a presumed lack of or occasional presence of receptors in these terminals to be 1% .
we set the power to 0.90 and the alpha to 0.05 ; using these parameters power analysis indicated a minimal sample size ( number of synapses to be analyzed ) of 23 . to estimate whether the type of target and of synapse formed by mglu7 and mglu8-containing terminals was different , the relative proportions were analyzed with the  test using the graphpad prism software ( version 5.0c ; graphpad software inc .
in order to confine the pharmacological activity of the mglu7 receptor agonist amn082 and mglu8 receptor agonist ( s)-3,4-dcpg to amygdala networks , mice were bilaterally implanted with microcanulae targeting the bla ( fig . 
mice exhibited no freezing behaviour in the context prior to the presentation of the first footshock ( first 2  min ) .
freezing levels constantly increased during conditioning to reach a maximum of approximately 40% ( fig . 
the maximum freezing level did not differ among experimental groups ( time effect : f5,150  =  64.106 , p  < 
0.001 ; treatment effect : f2,30  =  1.076 , p  =  0.354 ) ( fig . 
twenty - four hours later , mice were randomly assigned to one of the 3 treatment groups and received microinjections of either vehicle ( acsf ) , amn082 or ( s)-3,4-dcpg 15  min prior to the extinction trial . a significant treatment effect
( f2,29  =  8.028 , p  =  0.002 ) , time effect ( f29,841  =  12.513 , p  <  0.001 ) and treatment    time interaction ( f58,841  =  1.720 , p  <  0.001 ) was observed on extinction of contextual fear conditioning . in the conditioning context
were consistently decreased after 10  min of context exposure ( p  =  0.004 versus 2  min ) reaching less than 15% freezing after 20  min of context exposure ( fig . 
the mglu7 agonist amn082 accelerated the decline in freezing levels as their percentage of freezing was significantly attenuated already after 6  min of context exposure ( p  =  0.017 versus 2  min ) with an almost full extinction after 12  min ( fig . 
in contrast , in mice injected with ( s)-3,4-dcpg freezing levels increased up to 70% within the first 3  min of context exposure ( p  <  0.001 versus 1  min ) and stayed high for another 15  min ( fig . 
after this time point , mice began to show some reduction in freezing behaviour ( p  =  0.036 versus 2  min ) that , however , was incomplete and remained relative high ( 40% ) till the end of the trial ( fig . 
compared with control animals , ( s)-3,4-dcpg - injected mice displayed elevated fear responses during most of the time of the extinction trial ( fig . 
24  h later , mice from all three experimental groups did not differ in freezing levels displayed upon exposure to the conditioning chamber ( f2,27  =  0.070 , p  =  0.933 ; fig . 
in order to exclude that the impaired extinction observed after ( s)-3,4-dcpg - administration could be due to an effect of the drug on motor performance , we assessed the mice on the accelerating rotarod .
animals bilaterally implanted with canulae improved their motor performance over 15 training trials ( data not shown ) .
( s)-3,4-dcpg- and vehicle - treated mice did not differ in the latency to fall off the rod during the test trial at 20  min ( p  =  0.916 ) and at 40  min after microinjections ( treatment effect : f1,8  =  0.011 , p  =  0.920 ; time effect : f1,8  =  4.546 , p  =  0.066 ; treatment    time interaction : f1,8  =  0.018 , p  =  0.898 ) ( fig . 
these results indicate no general motor - impairing properties produced by intra - bla application of ( s)-3,4-dcpg .
although mobility scores , as indicated by the distance travelled in the testing chamber , showed a clear tendency to be reduced in ( s)-3,4-dcpg - treated mice as compared with vehicle - treated controls , no statistically significant treatment effect ( f1,8  =  3.898 , p  =  0.084 ) or treatment    time interaction ( f5,40  =  2.312 , p  =  0.062 ; fig . 
in contrast , vehicle- and ( s)-3,4-dcpg - treated animals differed in their freezing behaviour elicited by exposure to a novel context ( treatment effect : f1,8  =  7.901 , p  =  0.023 ; time effect : f5,40  =  8.141 , p  < 
0.001 ; treatment    time interaction : f5,40  =  6.219 , p  <  0.001 ) . while vehicle - treated animals showed no freezing responses to the conditioning context ,
( s)-3,4-dcpg induced per se high levels of freezing , which declined over time and was absent after 20  min spent in the conditioning chamber ( fig . 
2c ) . to elucidate the molecular and cellular underpinnings of the behavioural effects of mglu7 and mglu8 receptor agonists injected intra - amygdala , we have then investigated at both light and electron microscopic level the cellular and subcellular distribution of these receptors using highly specific antibodies .
we limited our analysis to the most abundant splice variants , namely mglu7a and mglu8a ( corti et  al . , 1998 ) .
however , this should not represent a limitation as the main splice variants for these receptors are generally coexpressed in the same brain regions ( corti et  al .
immunostaining for mglu7a receptors appeared punctate , suggestive of a presynaptic location , and to decorate the soma and dendrites of principal cells and interneurons in the bla as well as large neurons present at the outer border between the bla and itc clusters ( fig . 
similarly , mglu8a receptor labelling was particularly intense in boutons apposed to the dendrites of these large neurons ( fig .  4 ) .
in the basal nucleus of the amygdala ( ba ) , mglu7a immunoreactivity was much more prevalent than mglu8a , being most likely present in terminals of local axon collaterals of pyramidal neurons .
double immunofluorescence experiments allowed us to identify the large neurons as containing mglu1 receptors ( fig . 
3b  i ) , which correspond to previously described large itc neurons ( busti et  al . ,
the presynaptic location of mglu7a and mglu8a receptors was then established both in the ba and itc by means of silver - intensified immunogold pre - embedding electron microscopy .
for both receptors the labelling was restricted to the active zone of presynaptic axon terminals ( figs . 
k ) , which formed either asymmetric synapses on spines and dendrites or symmetric synapses on dendrites and somata . the type and frequency of synapses onto specific targets ( namely spines , dendritic shafts or somata ) was then analyzed in both the ba and itcs . in the ba , 68% of the randomly - selected synapses (
n  =  82 ) containing presynaptic mglu7a receptors were on spines , and of the remaining synapses , that were mostly present on dendrites , 62% were asymmetric .
in the itcs , mglu7a positive terminals ( n  =  43 ) targeted spines in only 28% of the cases , and of the synapses on dendritic shafts 71% were asymmetric .
the axon terminals containing mglu8a receptors in the ba ( n  =  41 ) made mostly ( 56% ) synaptic contacts with dendritic shafts , 30% of which formed asymmetric synapses , whereas the remaining synapses were on spines . in the itc , only 21% of the analyzed synapses ( n  =  95 ) having axon terminals immunolabelled for the mglu8a receptor were on spines , similar to what observed for mglu7a positive inputs , and 61% of the mglu8a - containing synapses on dendrites were asymmetric .
we could , therefore , observe a significant difference in the ba ( p  <  0.001 ,  test ) in the pattern of target innervation between mglu7a and mglu8a receptor - containing axon terminals , whereas in itcs no differences were detected ( p  =  0.22 ,  test ) .
these data suggest that in the ba these two mglu receptors may be to a large degree segregated in independent afferents , whereas in the itcs they could at least in part coexist . in order to understand whether mglu7a and mglu8a receptors indeed coexist in the same presynaptic axon terminals targeting large itc neurons , we performed double immunofluorescent experiments , which revealed a complex pattern of coexpression as some labelled boutons showed coexistence between mglu7a and mglu8a receptors whereas others appeared only labelled for mglu7a or mglu8a receptors , respectively ( fig .  5 ) . to assess whether the synapses on large mglu1+ itc neurons and containing mglu7a receptors in the presynaptic bouton were glutamatergic or gabaergic , we carried out triple - immunopre - embedding electron microscopy experiments .
we used silver - intensified immunogold reactions for detecting presynaptic mglu7a and postsynaptic mglu1 receptors and immunoperoxidase for visualizing vglut1 , vglut2 or vgat .
immunoreactivity for mglu7a was observed in gabaergic vgat+ inputs as well as in both vglut1 and vglut2 glutamatergic afferents ( fig . 
this indicates the presence of mglu7a receptors in afferents originated from thalamic / hypothalamic areas as well as from the neocortex / hippocampus and/or bla . to identify the origin of the glutamatergic inputs containing mglu7a and mglu8a receptors , we carried out tracing experiments injecting iontophoretically in mice the sensitive anterograde tracer pha - l combined with pre - embedding immuno - electron microscopy for revealing the presence of mglu7a or mglu8a receptors in the projections to the amygdala . because the mpfc plays a critical role in the expression and consolidation of fear extinction ( quirk and mueller , 2008 ) ,
we have injected pha - l into the mpfc . to our knowledge , no tracing studies describing the projection from the mpfc to the amygdala in the brain of any mouse strain have been published .
we show here two cases , selected from a larger series of injections , in which the injection site was mostly confined to the il - pfc ( fig . 
however , as a few labelled neurons could be observed in the prelimbic component of the mpfc , we will refer here our tracing as originating from the mpfc .
, 1996 ; vertes , 2004 ) , most of the fibres appeared to reach the amygdala through the medial forebrain bundle and then turning laterally through the substantia innominata .
projections from the mpfc terminated mostly in the intermediate division of the ba , although some labelled boutons could be observed in itc clusters placed along the intermediate capsule ( fig . 
other portions of the amygdala such as the bed nucleus of the stria terminalis intra - amygdala , the medial nucleus and some of the superficial nuclei received also a relatively dense projection . outside the amygdala , dense terminal and non - terminal projections were observed in the substantia innominata , nucleus accumbens , endopiriform nucleus , mediodorsal thalamus and lateral hypothalamus . by means of combined anterograde tracing and pre - embedding immuno - electron microscopic methods
, we have analyzed whether mpfc terminals forming synapses onto ba and itc neurons possessed presynaptic mglu7a or mglu8a receptors , and second whether terminals containing group iii mglu receptors formed synapses with mglu1-expressing neurons in these areas .
mpfc terminals formed only asymmetric synapses most of which ( 95% in the ba ; 98% in the itc ) with dendritic spines and the remaining with dendritic shafts . as shown in table  2 , none of the terminals immunolabeled for pha - l showed immunoreactivity for mglu7a or mglu8a receptors , suggesting that mpfc projections to the itc or ba do not contain these group iii presynaptic mglu receptors ( fig . 
similar to the mpfc , also for the thalamic output to the amygdala no studies are available for the mouse brain .
as thalamo - amygdala projections in the rat originate largely from midline nuclei ( ottersen and ben - ari , 1979 ; su and bentivoglio , 1990 ; turner and herkenham , 1991 ) , we have injected pha - l into the dorsal subdivision of the thalamic midline . the two cases presented here , selected from a larger series , showed little variability in the pattern of terminal labelling ,
therefore the descriptions below are representative for both animals . as shown in fig .  8a , the tracer injections involved mainly the paraventricular , intermediodorsal and central medial nuclei .
labelled axons leaving the injection site projected laterally to the inferior thalamic peduncles and entered the amygdala from its rostral pole .
the amygdaloid terminal zone was particularly intense in the intermediate division of the ba , moderate in the capsular and lateral divisions of the ce and weak in the medial division of the ce ( fig . 
a few labelled axons were also observed in the intermediate capsule and main nucleus of the itc ( fig . 
intense innervation was observed in the dorso - medial striatum and moderate in the ventral pallidum , accumbens , claustrum  endopiriform nucleus and cingulate cortex .
a combined anterograde tracing and pre - embedding immuno - electron microscopy approach was carried out also for pha - l labelled thalamic terminals .
thalamic terminals formed only asymmetric synapses most of which ( 90% in the ba ; 95% in the itc ) with dendritic spines , whereas the remaining were on dendritic shafts .
as shown in table  2 , approximately 23% of thalamic pha - l labelled terminals on spines in the itc possessed mglu7a receptors presynaptically , 29% of which formed synapses with mglu1 immunopositive profiles . in the ba , 33% of thalamic terminals with presynaptic mglu7a receptors contacting dendrites and 8% contacting spines
conversely , none of the terminals immunolabelled for pha - l showed immunoreactivity for mglu8a receptors , suggesting that also thalamic projections to the itc or ba do not contain presynaptic mglu8 receptors ( table  2 ; fig .  8) . to investigate
whether intrinsic projections from the ba possess group iii mglus and target itc neurons , we injected pha - l iontophoretically in the posterior parvicellular division of the ba ( fig . 
the terminal zone of labelled axons was most intense within the entire extent of the ba ( fig . 
several neurons were found intensely labelled for pha - l also in rostral areas of the ba and in the la , possibly due to retrograde transport of the tracer .
a robust projection was observed in the medial cea whereas the innervation of the itc seemed moderate to weak and mostly confined to the medial paracapsular cluster and main nucleus . a large bundle of labelled axons entered the stria terminalis to leave the amygdala . for this pathway ,
pha - l and mglu receptor immuno - electron microscopy was limited to the itc ( fig . 
although pha - l labelled ba terminals in the itc formed approximately 86% of synapses with dendritic spines , we observed a higher degree of synapses on dendritic shafts ( 14% ) , when compared to the other pathways analyzed .
of the shaft synapses 50% were symmetric , suggesting that some labelled interneurons of the ba anterogradely projected to the itc .
as shown in table  2 , 20% of ba labelled terminals in the itc possessed mglu7a receptors presynaptically , of these 50% were in boutons targeting the shafts and 24% the spines of mglu1 immunolabelled neurons .
a full understanding of how a pharmacological treatment influences a specific behavioural response requires , among other aspects , the identification of the brain areas and neural circuitries expressing the drug target . here
, we showed that the activation of mglu7 or mglu8 receptors through specific agonists , administered intra - amygdala before extinction training , produced different effects on the conditioned response .
the mglu7 receptor agonist amn082 facilitated extinction acquisition , whereas activation of mglu8 receptors by ( s)-3,4-dcpg prevented the reduction of the freezing behaviour normally observed by the re - exposure of the animals to the conditioning context in the absence of the unconditioned stimulus . despite the relatively similar distribution of the immunoreactivity for mglu7a and mglu8a receptors within the bla and itcs ( masugi et  al .
, 1999 ) , our anatomical analysis at the ultrastructural level now revealed how these two receptors are to a significant extent segregated to different inputs .
10 , which tries to summarise our anatomical findings , mglu7a receptors were located primarily onto glutamatergic afferents either intrinsic to the bla or extrinsic and arising from the thalamus , but not the mpfc . on the other hand
, mglu8a showed a more restricted distribution than mglu7a receptors in the ba and appeared absent from the three pathways that have been examined in this study .
the identified segregation of these two group iii mglu receptors into primarily distinct afferents corroborates the marked pharmacological difference caused by their respective agonists on extinction training . in the present study ,
the direct injection of amn082 and ( s)-3,4-dcpg into the bla pre - empted the complicated interpretation of systemic administrations and removed the risk of confounding actions of these compounds on peripheral group iii mglu receptors ( julio - pieper et  al . ,
based on previous studies , we can assume that the area in which these drugs have exerted their effect remains probably restricted to less that 1  mm around the injection site ( palazzo et  al .
therefore , the effect of amn082 and ( s)-3,4-dcpg should be confined primarily if not exclusively to the bla and the surrounding itcs .
moreover , we selected drug doses within the range of those previously used in other studies and shown to induce marked behavioural or physiological effects ( marabese et  al . , 2007 ;
on the other hand , given the relatively high concentration of amn082 and ( s)-3,4-dcpg in the vicinity of the injection site a possible off site effect , e.g. on other mglu receptors , can not be ruled out .
however , as the drugs were infused into the bla at a very low speed and 15  min prior to the extinction training , we believe that significant off site effects influencing extinction training are unlikely .
recently , group iii mglu receptors have received increasing attention as potential novel targets for anxiety disorders ( swanson et  al . , 2005 ) .
the overall effect mediated by these receptors appears to result from a complex balance between facilitatory and inhibitory actions that is dependent on their regulation of neurotransmitter release directly at the active zone of axon terminals .
indeed , we found that mglu7a and mglu8a receptors in the bla and itcs are exclusively located presynaptically in both glutamatergic and gabaergic terminals , similar to what observed in other brain areas ( ferraguti and shigemoto , 2006 ; ferraguti et  al . , 2005 ) . only in the last few years
, the individual role of distinct group iii mglu receptors has begun to emerge with the development of subtype selective drugs ( nicoletti et  al .
this study shows distinct roles of mglu7 and mglu8 in the expression of extinction and concurs with previous reports in delineating different functions for these receptors on a number of behavioural manifestations including pain modulation and anxiety ( marabese et  al .
, 2007 ; palazzo et  al . , 2008 ; ren et  al . , 2011 ) . our behavioural findings , which demonstrate a facilitated acquisition of extinction of contextual fear by amn082 , are in agreement with the observed delayed extinction learning in mglu7-deficient mice in both aversive and appetitive experimental paradigms ( callaerts - vegh et  al . , 2006
, as well as with the facilitated extinction of cue - induced fear - potentiated startle ( fendt et  al . , 2008 ) .
however , systemic administration of amn082 in rats has been recently shown to attenuate within - session extinction , but to facilitate between - session extinction retention ( toth et  al . , 2012 ) .
the limited facilitatory effect of amn082 on extinction acquisition observed in our study is likely due to the use of already well extinguishing mice .
since these animals show a very efficient extinction recall , it is difficult to demonstrate a further reduction of the freezing .
this view is supported by additional evidence obtained with oral administration of amn082 to 129/svimj mice , an animal model of impaired extinction ( hefner et  al . , 2008 ) .
in 129/svimj mice , amn082 produced a significant improvement of both acquisition and consolidation of fear extinction ( whittle , ferraguti and singewald , personal communication ) .
taken together our results substantiate the view that memories can be more easily inhibited when mglu7 receptors are activated ( fendt et  al . , 2008 ) .
one of the main conclusions of our study is that the facilitation of extinction occurs through the activation of mglu7 receptors located within amygdala networks .
previous anatomical evidence has demonstrated that in the bla mglu7 had the highest density of all group iii mglu receptor subtypes ( corti et  al . , 1998 ;
, we reveal the precise localization of mglu7 receptors in afferents to the ba and itcs . these were primarily glutamatergic inputs originating from ba pyramidal neurons and the midline thalamus , which contacted mostly spines .
however , the relatively high number of asymmetric synapses on dendrites made by mglu7 receptor - containing axon terminals suggests that they may originate from other brain areas with a preferential targeting onto dendrites .
the ba and itcs receive a prominent mesolimbic dopaminergic innervation ( fuxe et  al . , 2003 ) .
however , in  vivo microdialysis studies showed that systemic administration of amn082 did not influence extracellular dopamine ( da ) levels ( li et  al . , 2008 ) .
moreover , the ventral tegmental area and substantia nigra pars compacta possess negligible levels of mglu7 receptor mrna ( ohishi et  al .
these findings suggest that mglu7 receptors are not present on daergic terminals and that the facilitation of extinction by amn082 is a da - independent mechanism .
evidence from previous studies indicates that the modulation of the noradrenergic system within the bla facilitates the expression and consolidation of extinction of contextual fear conditioning ( berlau and mcgaugh , 2006 ; morris and bouton , 2007 ) .
the noradrenergic innervation of the bla arises mainly from the locus coeruleus in the brain stem ( fallon et  al . , 1978 ;
pickel et  al . , 1974 ; roder and ciriello , 1993 ) , a brain structure expressing very high levels of mglu7 receptor transcripts ( ohishi et  al . ,
moreover , noradrenergic axons in the bla form both asymmetric and symmetric synapses primarily with dendrites ( li et  al . , 2001 ) .
therefore , mglu7 receptors , besides glutamatergic and gabaergic terminals , may be located also presynaptically on noradrenergic afferents to the ba and itcs .
further studies addressing the distribution and role of mglu7 receptors on the noradrenergic system in the amygdala are warranted .
the localization of mglu7 receptors in a number of different afferents to both ba and itc neurons makes it difficult to relate the behavioural effects elicited by amn082 to a definite network . on the other hand , our study confines the presence of mglu7 receptors to a few defined inputs and rules out an action of amn082 on mpfc inputs , and in particular those of the il - pfc known to be important for the long - term storage of fear extinction memories ( quirk and mueller , 2008 ) .
the facilitation of extinction acquisition mediated by amn082 , besides being possibly due to an activity - dependent depression of neurotransmission ( perroy et  al . , 2002 ;
in fact , it has been shown that amn082 in addition to stimulating can induce the internalization of mglu7 receptors ( pelkey et  al . , 2007 ) .
however , since the selective downregulation of mglu7 receptor mrna expression by means of short interfering rnas inhibited the extinction of fear - potentiated startle , whereas amn082 given systemically facilitated extinction of conditioned fear ( fendt et  al . , 2008 )
the present study demonstrates that ( s)-3,4-dcpg , when injected intra - amygdala , precluded the attenuation of the freezing response during the extinction training in mice , but did not prevent the formation and consolidation of an extinction memory since the level of freezing in extinction recall was comparable to the control group .
( s)-3,4-dcpg also caused a reduced spontaneous locomotor activity and a short lasting immobility that was not due to motor impairment or sedation , as indicated by the rotarod test .
these behavioural effects suggest an anxiogenic - like activity of ( s)-3,4-dcpg that influenced fear behaviour and concealed the behavioural expression of fear extinction rather than impairing it .
interestingly , this seems in line with the impaired fear extinction shown by animals characterized by enhanced trait anxiety ( muigg et  al . , 2008 ) , which also closely mirrors findings in anxiety patients ( lissek et  al . , 2005 ) .
at odds with our findings , ( s)-3,4-dcpg was found to exert anxiolytic - like activity when given systemically in mice ( duvoisin et  al . ,
however , systemic application of ( s)-3,4-dcpg might have influenced a number of areas other than the bla ( gosnell et  al . , 2011 ) , and thus yielded a different net effect compared to a local injection .
no help for interpretation of our data can be gained from studies on anxiety - like behaviour in mglu8-deficient mice as they gave rise to largely conflicting results .
in fact , some of these studies reported an anxiogenic - like phenotype in mglu8-null mice ( duvoisin et  al .
, 2005 ; linden et  al . , 2002 ; robbins et  al . , 2007
) , whereas others found no effect of the genotype on measures of anxiety ( fendt et  al . , 2010 ;
injection of ( s)-3,4-dcpg in the rat bla showed no effect in the elevated plus maze and vogel test ( palazzo et  al . , 2008 ; stachowicz et  al . ,
2005 ) , but spontaneous locomotor activity and extinction of fear conditioning were not analyzed .
although these data might question an effect of ( s)-3,4-dcpg on measures of anxiety upon local infusion in the bla , we can not exclude that differences in doses , tests used and species contributed to these apparently incongruous findings .
our study reveals a more restricted distribution of mglu8a in the bla compared to mglu7a receptors , but a similarly intense decoration of large mglu1+ neurons at the border of itc clusters .
none of the traced pathways appeared to contain detectable amounts of mglu8a receptors , thus leaving still open the precise identification of the source of the inputs enriched in these receptors .
therefore , even though we can not confine the behavioural effects of ( s)-3,4-dcpg to a definite amygdala network , our work suggests that the mglu8a - containing inputs forming asymmetric synapses mostly arise from areas extrinsic to the amygdala , which could include the somatosensory , perirhinal and/or piriform cortex or the ventromedial nucleus of the hypothalamus .
in fact , among the few areas expressing significant amounts of mglu8a mrna ( corti et  al . , 1998 ;
1997 ) , these appear the only ones to provide a significant source of axons to the bla ( pitknen , 2000 ) . on the other hand , the large percentage of symmetric synapses on dendrites of bla neurons made by mglu8a - containing inputs may derive from local interneurons , consistent with the moderate expression of mglu8a mrna in the bla ( corti et  al . , 1998 ) .
further tracing studies combined with immuno - electron microscopy are needed to finally reveal the exact source of mglu8 + inputs .
one of the main findings of this work is the selective targeting by axon terminals enriched in group iii mglu receptors of the large mglu1+ neurons found at the border of itc clusters ( busti et  al . , 2011 ) .
the extensive dendritic arbors of these neurons mostly align along the intermediate capsule and are probably capable of receiving and integrating a large variety of inputs .
indeed , we have shown that the mglu7a receptor is present in both gabaergic and glutamatergic afferents , and that the latter ones form synapses primarily with the dendritic shafts , in agreement with the poorly - spiny nature of these itc neurons .
interestingly , both short- and long - term extinction , but not fear acquisition , was found impaired after injection of a mglu1 receptor antagonist into the bla ( kim et  al . , 2007 ) .
moreover , systemic pharmacologic blockade of mglu1 receptors before extinction retrieval facilitated relapse phenomena , such as renewal and reinstatement ( clem and huganir , 2010 ) . taken together
these data suggest that the activity of mglu1 receptors is linked specifically to mechanisms underlying fear extinction .
although the exact cellular and molecular mechanisms underlying the role of mglu1 receptors on extinction of fear conditioning remain to be established , our finding that large mglu1+ itc neurons are recipient of heavy innervation by inputs from the thalamus , ba and mpfc as well as by afferents enriched in group iii mglu receptors , which themselves influence fear extinction , identifies them as a potential key component of the neural networks underlying fear extinction .
however , these treatments appear of limited efficacy ( pull , 2007 ) underlying the need for treatments that address novel mechanisms . in addition ,
pharmacological treatments that could be used in adjunct to behavioural therapy to facilitate extinction would be of particularly clinical interest .
our study substantiates the view that mglu7 receptor agonists or positive allosteric modulators may represent such a class of drugs .
moreover , the elucidation of how and where , at the synaptic level , group iii mglu receptors may impinge on the neural pathways within the amygdala underlying inhibition of fear , will help determining the therapeutic value of drugs acting at these receptors .
 the group iii metabotropic glutamate ( mglu ) receptors mglu7 and mglu8 are receiving increased attention as potential novel therapeutic targets for anxiety disorders . 
 the effects mediated by these receptors appear to result from a complex interplay of facilitatory and inhibitory actions at different brain sites in the anxiety / fear circuits . 
 to better understand the effect of mglu7 and mglu8 receptors on extinction of contextual fear and their critical sites of action in the fear networks , we focused on the amygdala . 
 direct injection into the basolateral complex of the amygdala of the mglu7 receptor agonist amn082 facilitated extinction , whereas the mglu8 receptor agonist ( s)-3,4-dcpg sustained freezing during the extinction acquisition trial . 
 we also determined at the ultrastructural level the synaptic distribution of these receptors in the basal nucleus ( ba ) and intercalated cell clusters ( itcs ) of the amygdala . 
 both areas are thought to exert key roles in fear extinction . 
 we demonstrate that mglu7 and mglu8 receptors are located in different presynaptic terminals forming both asymmetric and symmetric synapses , and that they preferentially target neurons expressing mglu1 receptors mostly located around itcs . 
 in addition we show that mglu7 and mglu8 receptors were segregated to different inputs to a significant extent . in particular , mglu7a receptors were primarily onto glutamatergic afferents arising from the ba or midline thalamic nuclei , but not the medial prefrontal cortex ( mpfc ) , as revealed by combined anterograde tracing and pre - embedding electron microscopy . on the other hand , mglu8a showed a more restricted distribution in the ba and appeared absent from thalamic , mpfc and intrinsic inputs . 
 this segregation of mglu7 and mglu8 receptors in different neuronal pathways of the fear circuit might explain the distinct effects on fear extinction training observed with mglu7 and mglu8 receptor agonists.this article is part of a special issue entitled  metabotropic glutamate receptors. 
Introduction
Materials and methods
Results
Discussion
Conclusions