cancer   of the   ovary represents 30% of all malignancies of the female genital organs .
the most common ovarian neoplasms , typically occurring in women of reproductive age and    beyond , originate from the ovarian surface epithelium and belong to one of three major types : serous , mucinous , and endometrioid tumors .
these neoplasms range from the clearly benign ( 80% ) to highly malignant carcinomas , with tumors of borderline malignancy in between .  
bilateral carcinomas of the ovary vary in frequency depending on which tumor type is involved   but can be found in roughly 25% of all ovarian cancer cases .
the question of whether bilateral ovarian carcinomas are the result of metastatic spreading from one ovary harboring the primary tumor to the contralateral ovary , as opposed to the alternative , simultaneous occurrence of two independent primary tumors , was addressed nearly two decades ago by pejovic et al . .
in a chromosome banding analysis they found no clear - cut difference in the karyotypic pattern between the tumors of the two ovaries in each woman but considerable differences from case to case .
hence , metastatic spreading from one side to the other must have been the pathogenetic mechanism , although the side carrying the primary tumor could not be identified .
no similar later studies have been undertaken to confirm or falsify the findings and conclusions then made .
we present a series of 32 bilateral ovarian carcinoma cases , in six also including cancerous lesions in the omentum or peritoneum , analyzed primarily by karyotyping and high - resolution comparative genomic hybridization ( hr - cgh ) but also tested for microsatellite instability . because the findings in the two or three samples from each woman were largely similar ,
we conclude that bilateral ovarian cancer occurs by a metastatic process and that spreading to the contralateral ovary mostly is a late event in the clonal evolution of these cancers .
the examined material consists of 70 fresh     samples from ovarian carcinomas surgically removed at the norwegian radium   hospital from 1999 to 2004 ( see table   1 in supplementary material available online at   doi:10.1155/2010/646340 ) .
the tumors were part of a consecutive series of 248 ovarian tumors cultured and karyotyped by us ( of which 203 were carcinomas ; unpublished data ) .
the 70 tumors came from altogether 32 patients with bilateral ovarian cancer . from all patients we had tumor material from both ovaries , and from six patients we also had samples from a metastasis to the omentum ( three patients ) or peritoneal cavity ( three patients ) .
neoadjuvant therapy had been given to four patients before surgery ( cases 5 , 9 , 22 , and 24 ) ; otherwise , no preoperative chemotherapy or irradiation had been given .
the tumors were classified as serous papillary adenocarcinoma ( 22 cases ;   supplementary   table   1 ) , endometrioid carcinoma ( cases 23 , 29 , and 31 ) , adenocarcinoma nos ( case 24 ) , carcinosarcoma ( case 21 ) , and   mucinous adenocarcinoma ( case 26 ) , and four cases showed a mixed histology and were classified as endometrioid and serous papillary ( cases 3 , 6 , and 27 ) and clear cell and serous papillary carcinoma ( case 28 ) .
the tumors also showed different patterns of differentiation ( well , moderately , and poorly differentiated ; supplementary table   1 ) .
the tumor samples were manually minced and disaggregated with collagen ii ( worthington , freehold , nj , usa ) until a suitable suspension of cells and cell clumps was obtained .
after 6 - 7 days of culturing in a selective medium , colchicine was added and the cultures harvested according to mandahl .
the chromosomes of the dividing cells were then g - banded and a karyotype established according to the recommendations of the iscn .
chromosomes were karyotyped based on their inverted dapi appearance and the relative hybridization signal intensity was determined along each chromosome .
a negative ( normal versus normal ) and a positive ( a cell line with known copy number changes ) controls were included in the experiments .
for the scoring of cgh results , we adopted the use of dynamic standard reference intervals ( d - sri ) .
normal  ratio profile that takes into account the amount of variation detected in negative controls for each chromosome band .
this provides a more objective and sensitive scoring criterion than fixed thresholds [ 1012 ] and , consequently , a higher resolution .
the d - sri used was generated with data from 10 normal versus normal hybridizations ( totalling 110 cells ) .
this interval was automatically scaled onto each sample profile , and aberrations were scored whenever the case profile and the standard reference profile at 99% confidence intervals did not overlap . the description of the cgh copy number changes was based on the recommendation of the iscn .
the tumor 's microsatellite instability ( msi ) status was determined in all samples using a consensus panel of five microsatellite markers ( bat25 , bat26 , d2s123 , d5s346 , and d17s250 ) .
a tumor was considered to be msi - high if two or more of the five markers exhibited novel alleles compared to normal dna , msi - low if only one marker deviated from the normal pattern , and microsatellite stable ( mss ) if none of the tumor genotypes showed an aberrant pattern .
control dna corresponding to the individual tumors was not available from the patients and therefore single allele changes ,   that is , the presence of two different alleles , can reflect a heterozygous constitutional genotype or a homozygous genotype with a novel tumor - specific allele .
thus , dinucleotide markers were not scored when such a pattern appeared in the tumors .
allelic sizes were determined using genemapper 3.7 software ( applied biosystems , foster city , ca , usa ) , and the results were independently scored by two investigators .
the cell culturing and subsequent g - banding cytogenetic analysis gave informative results in 58 samples ( supplementary   table   1 ) , 39 of which showed an abnormal karyotype whereas 19 were normal .
the remaining 12 samples were culture failures and therefore could not be examined using this technique .
the abnormal karyotypes were complex ; that is , more than four abnormalities were present in all informative cases but one , case 21 .
this case was the only carcinosarcoma of our series ; the tumor of the right ovary ( case 21b ) showed a   der(16)t(1;16)(q21;q22 ) as the sole abnormality whereas a complex karyotype was seen in the tumor of the left ovary ( case 21a ) , but with a similar der(16)t(1;16)(q21;q22 ) as one of many changes .
mostly many more aberrations than four were seen , several of which could not be completely identified .
the modal chromosome number was hypodiploid in six cases , diploid in three , hyperdiploid in three , hypotriploid in one case , hypertriploid in 19 , neartetraploid in one , hyperpentaploid in three cases , and a mix of hyperdiploid and hypertetraploid clones in one ( case 5a ) . for 14 cases
, we obtained abnormal karyotypes from either both ovaries or from one ovarian tumor and a metastasis in the omentum / peritoneum , but in six of these cases , the technical quality was not sufficiently good to be able to compare the karyotypic data from different lesions in a reliable way . for eight patients , however , it was possible to compare the abnormal karyotypes , and in one additional patient both ovarian tumors and the peritoneal metastasis yielded informative results and could be compared ( cases 8a , 8b , and 8c in supplementary table   1 ) . in all these nine cases ,
more or less extensive karyotypic similarities between the samples taken from the different tumor lesions were found , with the number of identical aberrations ranging from one to eight per karyotype .
however , because in seven out of these nine cases the karyotypic description was incomplete due to the complexity of the rearrangements , it is possible that more common abnormalities were present . in cases
19a and 19b , the karyotypic description was identical for the two tumorous ovaries ( figure 1 ) .  
the chromosomes seen to be most frequently involved in structural rearrangements by g - banding analysis were , in order of falling frequency , chromosomes 19 , 1 , 11 , and 16 ( figure 2 ) .  
the bands most frequently rearranged were 19p13 ( involved in 12 rearrangements ) , 19q13 ( 11 rearrangements ) , 1q21 , 16q22 , and 19q10 - 11 ( six rearrangements ) , 11p15 , 12p13 , and 15p11 ( five rearrangements each ) , and 1p36 and 16q24 ( four rearrangements each ) .
the chromosomes most frequently involved in numerical aberrations were the x chromosome ( in eight tumors ) and chromosomes 8 and 14 , involved in six tumors each .  
the hr - cgh gave informative results on 60 samples showing genomic imbalances in 56 of them . from seven lesions
no informative results were obtained in cases 13b , 22b , and 32b because of poor quality of the hybridization signal , despite running the experiments twice . in six cases ,
however , because the g - banding analysis often showed an incomplete karyotype with marker chromosomes and additional material of unknown origin sitting on known chromosomes , the cgh analysis allowed the identification of more imbalances . in 17 cases , a normal karyotype was detected by g - banding analysis whereas the cgh experiments showed genomic imbalances in the tumor samples .
we gained information also on the 10 cases that were culture failures , finding imbalances in seven of them .
gains were more frequent than losses as seen by hr - cgh , and high - level amplification was found in 23 lesions .
the major copy number changes were gains of or from chromosome arms 1p , 1q , 2p , 3q , 5p , 8q , 11q , 12p , and 20q and losses of or from xp , 4q , 5q , 6q , 8p , 13q , 16q , 17p , 17q , 18q , 19q , and 22q .
more specifically , the most frequently gained bands were , in order of decreasing frequency , 5p14 and 8q23 ( 39% ) , 2p23 ( 38% ) , 1q24 , 3q25 , and 3q27q28 ( 36% ) , 1q21 , and 3q22 ( 34% ) , 2p13 , 3q13 , and 8q21 ( 32% ) , 1p31 ( 30% ) , 20q13 ( 29% ) , and 11q22 and 12p12 ( 25% ) .
the most frequently lost bands were 17p11 and 17p13 ( 45% ) , 17p12 ( 43% ) , 16q23 and 22q13 ( 38% ) , 8p21 and 17q21 ( 34% ) , 8p22 - 23 ( 32% ) , xp21 and 6q25 ( 30% ) , 4q34 , and 18q22 ( 29% ) , 13q14 and 19q13 ( 27% ) , and 5q13 - 14 ( 25% ) ( figure 3(a ) ) .
a comparison of the imbalances scored for the tumors in the two ovaries and/or the omentum / peritoneum showed that the bands most often gained by both ovarian tumors were 5p14 ( 70% of the 23 cases or 46 samples showing informative results ) , 8q23 - 24 ( 65% ) , 1q23 - 24 ( 57% ) , 12p12 ( 48% ) , 2q23 and 3q22 ( 43% ) , and 2p23 , 3q13 - 21 , 3q24 - 28 , and 11q14 ( 39% ) .
the most often lost bands were 17p11 ( 78% ) , 17p13 ( 74% ) , 17p12 ( 70% ) , 22q13 ( 61% ) , 8p21 and 19q13 ( 52% ) , 8p22 - 23 ( 48% ) , 16q22 - 23 , 17q12 - 21 , and 18q22 ( 43% ) , and 4q31 , 4q33q34 , 11p15 , and xp21 ( 39%
the samples showed from one ( samples 4a and 4b ) to 58 ( sample 11a ) copy number alterations with an average number of copy alterations ( anca ) index of 37.9 .
amplifications were most often scored on chromosome arms 8q ( eight tumors )   and 3q and 12p ( four tumors each ) . as ovarian cancer can be part of the hereditary nonpolyposis colon cancer ( hnpcc ) spectrum , characterized by microsatellite instability ( msi ) ; we tested for this in the present series .
fifty - six of the tumors were classified as microsatellite stable ( mss ) as none of the tumor genotypes showed an aberrant pattern ; these were mss tumors .
three cases ( 9a , 9c , and 16b ) were scored as msi - low ( msi - l ;   supplementary table   1 ) . in four cases ,
cytogenetic studies of bilateral ovarian cancer are limited to the one by pejovic et al .   who karyotyped tumors from both ovaries in 15 patients .
because the baseline karyotypes in each tumor pair were identical in the 11 patients from whom informative results were obtained , the conclusion was that the second tumor always arose by spreading of a monoclonal process from the first one . however , since the clonal evolution of the neoplastic cells in the two locations was similar , one could not determine which tumor was primary and which was metastatic .  
we report a series of 32 patients with bilateral ovarian cancer analyzed by karyotyping , hr - cgh , and a microsatellite instability assay . as in the study by pejovic
, considerable similarity was observed between the left - sided and right - sided tumors . from one to eight common aberrations
were seen by karyotyping in the nine patients from whom informative results for both ovaries / omentum / peritoneum were obtained , averaging 3.22 common aberrations per patient . because the karyotypic descriptions were incomplete
indeed , the hr - cgh analysis revealed from one ( cases 4 and 31 ) to 43 ( case 1 ) genomic imbalances common to both or all lesions in the 23 patients from whom informative results for both ovaries / omentum / peritoneum were obtained , giving an average of 25.5 common aberrations per case or patient .
that bilateral ovarian cancer occurs   via a metastatic mechanism , but the   addition of cgh data allowed us to expand on this assessment of the pathogenetic connection between macroscopically discrete tumor lesions : the fact that so many aberrations are common to the tumors in both sides indicates that spreading to the contralateral ovary is a late event in the clonal evolution of the neoplastic parenchyma cells .
the aberrations unique to each tumor lesion , on the other hand , in all likelihood arose after the metastasis was set up , in the other - sided ovary or in the omentum / peritoneum .
the data are too sparse to conclude with certainty whether the latter metastases differ in any significant way from the tumors situated in the ovaries themselves when it comes to acquired genomic aberrations , but this does not appear to be the case .    in the present study ,
the chromosomes most frequently involved in numerical aberrations were the x chromosome and chromosomes 8 and 14 .
more precisely , the x chromosome was lost in six tumors and gained in two , whereas chromosomes 8 and 14 were involved in numerical aberrations in six tumors each , equally often in gains and losses .
the chromosome most frequently involved in structural rearrangements was chromosome 19 ; more specifically , 19p13 was involved in 12 rearrangements in eight tumors , whereas 19q13 was involved in 11 rearrangements in eight tumors , and the centromeric region of chromosome 19 was involved in six rearrangements in three cases .
alterations of 19p13 particularly , but also 19q13 , in the form of added extra material are known to be among the most frequent cytogenetic aberrations in ovarian carcinomas [ 4 , 1618 ] .
sometimes the 19p+ or 19q+ markers look alike [ 16 , 19 ] , but the origin of the additional material could only rarely be identified .
the rearrangements of chromosome 19 have never been seen as the only chromosomal aberration in ovarian carcinoma , and so it seems likely that they are progressional rather than primary anomalies .
their detection here in many cases indicates that they show no frequency - difference between unilateral and bilateral ovarian carcinomas .
rearrangements of chromosome 1 were also detected quite often in the present series , involving mostly 1q21 ( six rearrangements ) and 1p36 ( four rearrangements ) .
[ 16 , 19 ] found frequent deletions of the distal half of 1q and various abnormalities resulting in loss of 1p34 - 36 .
again , no difference between the current series of bilateral carcinomas and ovarian carcinomas in general is discernible .  
another hot - spot of chromosomal rearrangements in our series was 11p14 - 15 ( eight rearrangements in total , five mapped to 11p15 and three to 11p14 ) .
often the rearrangements are described as an add(11)(p14 - 15 ) which , in addition to the added material , may well also lead to loss of 11p - material distal to the breakpoint .
similar changes have been reported by other investigators [ 16 , 20 , 21 ] .
also chromosomal bands 16q22 and 16q24 were repeatedly rearranged in the present series , in six and four cases , respectively .
the mitelman database of chromosome aberrations in cancer reports such changes in five and 14 ovarian carcinomas , respectively . as for the other above - mentioned changes , neither the genes involved nor anything else about the mechanism of their contribution to tumorigenesis is known .  
the karyotypic features of the only bilateral carcinosarcoma analyzed in the present series deserve special mention .
the tumor of the left ovary showed two apparently unrelated clones with many abnormalities in an incomplete karyotypic description .
the right - sided tumor had a 46,xx , der(16)t(1;16)(q21;q22 ) as the sole abnormality in all analyzed cells , an aberration that was shared also by the contralateral tumor .
in contrast to what appears to be the general rule in the other bilateral tumors , therefore , spreading to the left side seems to have taken place early in clonal evolution in this case .
unfortunately , no dna was available to perform cgh on the tumor from the right side of this case to compare the genomic imbalances of the two lesions .
they are microscopically characterized by a mixture of malignant epithelial and stromal element , similar to what is observed in corresponding uterine tumors .
rearrangement of chromosome 1 seems to be the most frequent cytogenetic change in carcinosarcomas of the ovary as well as of the uterus .
the bands most often seen by hr - cgh to be gained in both tumorous ovaries were 5p14 ( 70% of the 23 cases or 46 samples ) , 8q23 - 24 ( 65% ) , 1q23 - 24 ( 57% ) , 12p12 ( 48% ) , 2q23 and 3q22 ( 43% ) , and 2p23 , 3q13 - 21 , 3q24 - 28 , and 11q14 ( 39% ) .
the most often lost bands were 17p11 ( 78% ) , 17p13 ( 74% ) , 17p12 ( 70% ) , 22q13 ( 61% ) , 8p21 and 19q13 ( 52% ) , 16q22 - 23 , 17q12 - 21 , and 18q22 ( 43% ) , and 4q31 , 4q33 - 34 , 11p15 , and xp21 ( 39% ) .
this picture of imbalances by and large tallies well with what has been reported before in unilateral ovarian carcinomas .
the most common imbalances detected in ovarian carcinomas by chromosome - based cgh have been gains of or from chromosome arms 1q , 3q , 8q , 12p , and 20q and losses of or from 4p , 4q , 8p , 13q , 16q , 18q , and xp [ 3139 ] .
interestingly , the chromosomal band most frequently gained in our series of bilateral ovarian carcinomas was 5p14 , being gained in 70% of the cases .
this is the first time that the short arm of chromosome 5 is seen to be gained so often in ovarian carcinomas .
the two only previous reports in which the same band was found gained showed a frequency of 17.6% ( three out of 17 sampled analyzed ; ) and 41.1% ( six out of 13 samples analyzed ; ) . in addition , a cgh profile of 543 cases of ovarian carcinomas ( summarized in http://www.progenetix.net/ ) shows a profile for 5p14 with 14.4% gains , 3.9% losses , and 1.3% amplifications .
an explanation for the higher frequency of gain of 5p14 observed in our series could be found in the fact that our data are based on high - resolution cgh , a method that is known to be more sensitive in the detection of imbalances compared to normal chromosomal cgh .
the most exciting possibility , of course , would be that the observed gain has to do with a tumor 's propensity to spread to the contralateral side , but confirmation in independent studies is needed before we ascribe much credibility to this explanation .
regardless of what it might mean , the direct pathogenetic significance of this specific gain remains unknown ; possibly oncogene(s ) located in 5p14 may be active in ovarian carcinogenesis and/or tumor progression and spreading .
the most frequent losses seen in the present series were from 17p , more precisely 17p11 in 78% of the samples , 17p13 in 74% , and 17p12 in 70% .
much interest has focused on the loss of genetic information from the short arm of chromosome 17 , where losses seem to occur especially at 17p13.1 [ 42 , 43 ] as well as at an even more distal locus in 17p13.3 [ 42 , 44 ] .
two possible target tumor suppressor genes have been mapped to 17p13.3 , ovca1 and ovca2 .
indeed , loss at 17p is the most common genetic alteration thus far detected in ovarian cancer , with mutation rates as high as 50% in advanced stage carcinomas .
however , we did not perform any further , detailed analysis to see if tp53 or possibly other gene(s ) were directly involved in the losses detected in this series .
dna microsatellite instability reflects an altered pattern of short tandem repeat sequences ( microsatellites ) in dividing cells and has been described in hnpcc as well as other tumor types .
ovarian cancer , although most often sporadic , can occur together with hnpcc as part of the lynch cancer family syndrome .
several studies have suggested an association between msi and certain histological types of ovarian carcinoma .
however , in most of these studies , different kinds of microsatellite markers were used , and the presence of msi was declared based on the demonstration of instability at only one locus [ 4852 ] . in studies where the nci markers and criteria were used , sood et al .   found msi - h in 12% of invasive carcinomas , whereas gras et al .  
reported that msi - h was limited to endometrioid and clear cell carcinomas ( 12.5% ) .
found that 20% of endometrioid carcinomas were msi - h . in our series of bilateral ovarian carcinomas , where 22 of 32 were serous papillary tumors , all cases but one ( tumors 9a and 9c ; these tumors were serous papillary carcinomas and
this is in agreement with previous studies finding that most of the msi were scored in tumors of the clear cell and endometrioid subtype of ovarian cancer .
 cancer of the ovary is bilateral in 25% . 
 cytogenetic analysis could determine whether the disease in bilateral cases is metastatic or two separately occurring primary tumors , but karyotypic information comparing the two cancerous ovaries is limited to a single report with 11 informative cases . 
 we present a series of 32 bilateral ovarian carcinoma cases ,   analyzed by karyotyping and   high - resolution cgh . 
 our karyotypic findings showed that spreading to the contralateral ovary had occurred in bilateral ovarian cancer cases and that it was a late event in the clonal evolution of the tumors . 
 this was confirmed by the large number of similar changes detected by hr - cgh in the different lesions from the same patient . 
 the chromosomal bands most frequently involved in structural rearrangements were 19p13 ( n = 12 ) and 19q13 ( n = 11 ) . 
 the chromosomal bands most frequently gained by both tumorous ovaries were 5p14 ( 70% ) , 8q23 - 24 ( 65% ) , 1q23 - 24 ( 57% ) , and 12p12 ( 48% ) , whereas the most frequently lost bands were 17p11 ( 78% ) , 17p13 ( 74% ) , 17p12 ( 70% ) , 22q13 ( 61% ) , 8p21 and 19q13 ( 52% ) , and 8p22 - 23 ( 48% ) . 
 this is the first time that 5p14 is seen gained at such a high frequency in cancer of the ovary ; possibly oncogene(s ) involved in bilateral ovarian carcinogenesis or tumor progression may reside in this band . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion