first approved for use in 1988   the applications of liquid fibrin sealant are expanding with current guidelines indicating improved safety and efficacy of two - component fibrin sealant products such as tisseel vh ( baxter , westlake village , ca , usa ) [ 2 , 3 ] .
[ 4 , 5 ] fibrin sealants have been used for urologic application including partial nephrectomy ( laparoscopic and open surgical procedures ) , which takes advantage of the hemostatic properties [ 57 ] , and during hypospadias surgery , fistula repair , and urethroplasty , which employ the sealant properties . [ 6 , 8 , 9 ] more recently , use of fibrin sealant at the urethrovesical anastomosis in radical retropubic prostatectomy has been employed to facilitate earlier catheter removal   and decreased urinary leakage .
no studies have been done to date expanding this use into robotic surgery but , by inference , application of fibrin sealant at the urethrovesical anastomosis during ralrp may offer advantages . at our institution ,
patients undergoing robotic assisted laparoscopic radical prostatectomy are routinely discharged on post - operative day one with minimal morbidity . the protocol for post - operative care
pratt drains are removed prior to discharge if volumes remain less than 60  cc after ambulation .
foley catheters are removed at one week if a cystogram shows no leak . in our study , we questioned whether the application of tisseel vh , a two component fibrinogen and activated thrombin sealant , at the anastomosis of the urethra and bladder after radical robotic prostatectomy confers any advantages .
after completion of the first 100 cases , to minimize the effect of the learning curve , 40 consecutive men underwent ralp by one surgeon ( wds ) at our institution .
robotic assisted laparoscopic radical prostatectomy was performed in the standard transperitoneal fashion in the manner described in 2002 by menon [ 13 , 14 ] after prostate removal , the urethrovesical anastomosis is sutured in a running fashion with 3 - 0 monocryl .
an 18 french silicone foley catheter is placed prior to the completion of the anastomosis and the balloon inflated with 12  cc of normal saline .
the bladder neck and urethra are approximated over the catheter prior to the final anastomotic sutures and any slack in the running suture is removed .
the first 20 men had the running sutured anastomosis without addition of fibrin sealant and served as the control group .
the second group of 20 men underwent the running anastomosis in the same standard fashion with addition of 6  cc fibrin sealant tisseel vh in an omega pattern starting at the right posterior and moving to the left posterior border .
both groups of patients had a 15 french round jp drain placed at the urethrovesical anastomosis , via the 5  mm suction port , at the completion of the case . 

1fibrin sealant applied over the urethrovesical anastomosis fibrin sealant applied over the urethrovesical anastomosis patients were discharged to the post anesthesia care unit and then to the surgical floor where output from the jp drain was measured for eight - hour intervals .
drains were removed when output was less than 60  cc per shift after ambulation .
volume of jp output per shift , date of jp removal , and date of discharge were recorded for each patient and obtained from the medical record and bedside chart .
hundred and fifty milliliters of dilute omnipaque contrast material were instilled under gravity per catheter under fluoroscopic guidance . if leak was demonstrated the procedure was repeated a week later .
date of removal of foley catheter was recorded as was presence or absence of anastomotic leak .
data were collected in a retrospective chart review for factors that would influence tissue healing , including smoking history , use of steroids , and presence of diabetes .
final pathologic staging was determined by the pathologist at the time of accessioning as was gleason grade on the prostate specimen .
mean , standard deviation , and standard error were calculated for the data in each group .
the control group and the tisseel vh group were matched for age , preoperative psa , gleason grade , prostate size , and pathologic stage at removal .
there were no statistically significant differences between the two groups ( table  1 ) . 

table  1comparison of control and study groups for age , psa , gleason grade , pathologic stage , and prostate sizecontrol grouptisseel groupage  mean60.55se 1.75858.25se 1.285 
 p  =  0.29  range48734666psa  mean5.23se 0.454.708se 0.64 
 p  =  0.51  range3.110.20.3611.96gleason grade  mean6.3se 0.1286.5se 0.115 
 p  =  0.251  range(3  +  2  =  5)(4  +  3  =  7)(3  +  3  =  6)(4  +  3  =  7)pathologic stage  pt2a46  pt2b10  pt2c1113  pt3a41prostate size  mean51.7se 3.20847.73se 3.021 
 p  =  0.37  range3182.633.9285 comparison of control and study groups for age , psa , gleason grade , pathologic stage , and prostate size the jackson  pratt drain was removed on mean pod 2.1 ( range 113 se 0.632 ) in the control group and mean pod 1.3 ( range 13 se 0.147 ) in the tisseel group ( p  =  0.23 ) .
this was equivalent to a mean of 3.8 nursing shifts ( range 28 se 0.484 ) in the control and a mean of 3.1 nursing shifts ( range 28 se 0.347 ) in the tisseel group ( p  =  0.24 ) .
patients were discharged on pod 1.4 ( range 13 se 0.152 ) in the control , and pod 1.3 ( range 13 se 0.147 ) in the tisseel group ( p  =  0.64 ) . for the control group
for the tisseel group the cc per shift jp output was 38.65 ( range 0100 se 4.84 ) ( p  =  0.26 ) .
catheter removal was on pod 12.1 ( range 726 se 1.397 ) in the control group . in the tisseel group
the catheter was removed on pod 9.75 ( range 718 se 0.602 ) ( p  =  0.13 ) ( table  2 ) . 

table  2comparison of control and study groups for drain removal , date of discharge , date of catheter removal , and cc per shift of drain outputcontrol grouptisseel group 
 p valuejp drain removal pod #  mean2.1se 0.6321.3se 0.147 
 p  =  0.23  range11313jp drain # shifts  mean3.8se 0.4843.1se 0.347 
 p  =  0.24  range2828discharge pod #  mean1.4se 0.1521.3se 0.147 
 p  =  0.64  range1313catheter removal pod #  mean12.1se 1.3979.75se 0.602 
 p  =  0.130  range 726718cc per shift  mean63.24se 20.7538.65se 4.84 
 p  =  0.26  range84350100 comparison of control and study groups for drain removal , date of discharge , date of catheter removal , and cc per shift of drain output one patient in the tisseel group failed one cystogram trial , and no patient was readmitted with large - volume leak or ileus . in the control group ,
two patients failed one cystogram trial , one patient failed two cystogram trials , and two patients were readmitted with large - volume drain / drain site output and ileus table  3 . 

table  3comparison of factors affecting healing and rate of ileus in the control and study groupscontrol grouptisseel groupcystogram leak 51ileus20tobacco use  history810  current11steroid use00diabetes1 ( diet controlled)0 comparison of factors affecting healing and rate of ileus in the control and study groups factors potentially contributing to poor wound healing and thereby affecting results were also taken into account ( table 3 ) . smoking history , steroid use , and diabetes diagnosis were all retrospectively reviewed . in the control group : one patient had diet - controlled diabetes , no patient had a history of steroid use , eight patients were former smokers , and one patient was a current smoker . in the tisseel group : no patient had diabetes , no patient had a history of steroid use , ten patients were former smokers , and one patient was a current smoker .
first introduced for use in cardiothoracic surgery in 1988 , the use of fibrin sealant and other tissue sealant and hemostatic products has expanded across surgical disciplines in the last two decades . in urology , fibrin sealants are the most commonly employed , and though many applications are off - label uses , there has been mounting evidence for their usefulness in urology [ 611 ] .
report that use in radical retropubic prostatectomy decreases perioperative drain output , and suggest that in some patients undergoing traditional open radical prostatectomy application of fibrin sealant could accelerate discharge .
morey et al . have applied fibrin sealant in simple retropubic prostatectomy and those patients required no perioperative drain .
novel uses of fibrin sealant in urology utilize its use as a hemostatic adjunct in partial nephrectomy and renal trauma , and as a sealant in hypospadias , ureteral anastomoses , and urethroplasty [ 6 , 8 ] .
several case reports and small series exist supporting the use in pyeloplasty , vasovasostomy , ureteral anastomosis , and open radical prostatectomy . in our study
we expand the potential use to include reinforcing the urethrovesical anastomosis in ralrp . already shown to reduce drain output in the open radical prostatectomy   and to assist in the two - sutured anastomotic technique described by gallo et al , tisseel s use in this realm
seems a natural progression given the minimally invasive nature of the procedure and reduced hospital stay . in our study ,
we were not able to find a statistically significant difference in jp drain output or days with jp drain , likely because most patients with jp drains have them removed after a relatively short interval .
jp was removed in 2.1 versus 1.3  days in the control and tisseel groups ( p  =  0.23 ) .
similarly discharge home was not statistically different with most patients able to leave on pod 1 .
days in the hospital were 1.4 in the control group and 1.3 in the tisseel group .
catheter removal was earlier in the study group 9.75 versus 12.1  days , with a trend toward statistical difference ( p  =  0.13 ) ( table  2 ) . where the use of fibrin sealant was most noticeable was at follow up cystogram and catheter removal .
once tisseel was introduced , only one patient had leak on cystogram and had to return for a repeat cystogram compared to six in the control group .
no patient was readmitted with leak or ileus in the study population while two in the control group were readmitted with ileus .
we examined other factors that could account for poor tissue healing including smoking , steroid use , and diabetes and found no significant differences .
we conclude that application of the fibrin sealant aids in healing and sealing the anastomosis and accounts for the reduced leakage .
use of fibrin sealant in urology can be expanded to include reinforcing the suture line in the urethrovesical anastomosis in ralrp .
application of fibrin sealant at the urethrovesical anastomosis appears to facilitate sealing , thereby allowing earlier removal of the jp drain by 0.8  days and the foley catheter by 2.35  days in patients undergoing ralrp .
further study to validate the procedure should be undertaken and a cost - effective approach to use should be employed .
 leakage at the urethrovesical anastomosis in the post - operative period can result in morbidity including ileus . 
 we examined the effectiveness of using a fibrin sealant at the anastomosis to limit urine leakage thereby facilitating earlier jackson  pratt drain and foley catheter removal following robotic assisted laparoscopic prostatectomy ( ralrp ) . 
 forty consecutive patients underwent ralrp by one surgeon at our institution . 
 the first 20 patients underwent standard operation and served as the control group . 
 the subsequent 20 patients underwent the same operation with addition of fibrin sealant following a running absorbable sutured urethrovesical anastomosis . 
 the two groups were compared for age ( 60.5 vs. 58.2  years ) , pre - operative psa ( 5.23 vs. 4.71 ) , gleason score ( 6.3 vs. 6.5 ) , stage at resection , and prostate size at resection ( 51.7 vs. 47.7  g ) . 
 wilcoxon rank sum test determined no statistically significant differences in the groups . 
 patients in the fibrin sealant group had 1.3 versus 2.1  days with a jackson  pratt drain , 9.75 versus 12.1  days with a catheter , and an average of 38.6 versus 63.2  cc of drainage per shift . 
 catheters were removed when a cystogram demonstrated no extravasation of contrast . 
 two patients in the control group and no patients in the fibrin sealant group had large - volume leakage and ileus post - operatively . in patients undergoing ralrp , 
 application of fibrin sealant at the urethrovesical anastomosis appears to facilitate sealing , thereby allowing earlier removal of the jp drain , by 0.8  days , and the foley catheter , by 2.35  days , than in controls . 
 no patients in the fibrin sealant group suffered post - operative ileus . 
 this adjunct may be especially useful early in the learning process to reduce morbidity . 
Introduction
Materials and methods
Results
Discussion
Conclusion