 a simple and rapid reversed phase - high performance liquid chromatographic method was developed for simultaneous determination of imipramine hydrochloride and diazepam in pharmaceutical formulations . 
 the elution was done in isocratic mode utilizing a mobile phase consisting of methanol : water:0.1 m sodium acetate ( 30:50:20 v / v / v ) on chromosil c18 column with a flow rate of 1.0 ml / min and with detection at 243 nm . 
 the measured retention time was 3.330.02 min for imipramine hydrochloride and 4.640.02 min for diazepam . 
 linearity was measured in the range 25 - 150 g / ml for imipramine hydrochloride ( r2=0.999 ) and in the range 5 - 30 g / ml for diazepam ( r2=0.9994 ) , respectively . 
 the limits of detection and quantitation were 0.03 and 0.1 g / ml for imipramine hydrochloride and 0.02 and 0.07 g / ml for diazepam . 
 satisfactory validation was also obtained from recovery ( 100.95 - 101.52% for imipramine hydrochloride and 99.47 - 100.33% for diazepam ) studies , intraday and interday precision ( < 2% ) and robustness results . 
 the reported method was the first study of these drugs in combination and could be employed for routine quantitative determination of imipramine hydrochloride and diazepam in tablets . 
Financial support and sponsorship:
Conflict of interest: