patients ( n=96 ) were recruited from our hospital - based dental clinic as a subset of a previous clinical bacteremia study ( 8 , 12 ) .
patients were excluded from the study if they had fewer than 10 teeth , had taken a systemic antibiotic within 2 weeks prior to the study , or were immunocompromised .
patient demographics and clinical characteristics are summarized in table 1 
 ( 8) . as changes of clinical parameters were not the focus of this study , we kept the description here as basic . demographic and clinical characteristics of study cohort continuous values are mean ( sd ) , discrete values are number [ percentage ] .
calculus index : mean score for all tooth scores based on scale : 0=no calculus ; 1=supra - gingival ( not more than 1 mm ) ; 2=moderate supra - gingival and/or sub - gingival calculus ; and 3=abundance of supra - gingival and sub - gingival calculus .
gingival index : mean score of all tooth scores based on scale : 0=normal gingiva ; 1=mild inflammation , a slight change in color and edema , and no bleeding on probing ; 2=moderate inflammation , redness , edema , and bleeding on probing ; and 3=severe inflammation , marked redness and edema , ulcerations , and a tendency toward spontaneous bleeding .
plaque index : mean score of all tooth areas scores ( mesial , distal , facial , and lingual ) based on scale : 0=no plaque in the gingival area ; 1=no plaque visible to the unaided eye , but plaque is visible on the probe after being moved across the gingival crevice ; 2=gingival area covered with a thin to moderately thick layer of plaque visible to the naked eye ; and 3=heavy plaque accumulation and soft debris in the interdental area ( 8) .
dental plaque samples were collected at least 1 h before any dental manipulation , such as eating , tooth brushing , or tooth extraction .
they were acquired by scraping a dental scaler across the supra - and superficial sub - gingival aspect of 14 teeth , with the deepest probing depths , and pooled .
the sample was suspended in te buffer ( 50 mm tris hcl , 1 mm edta ph 7.6 ) , transferred on ice , and stored at 80c until furthzer analysis .
bacterial dna extraction was performed using a modification of qiaamp dna mini kit ( qiagen , valencia , ca ) , as described previously ( 12 ) and used for simultaneous analysis by homim and homings .
16s rrna gene sequences were polymerase chain reaction ( pcr ) amplified and labeled with cy3-dctp in a second nested pcr .
individual intensity signals were normalized by comparison to the average signals from universal 16s rrna gene probes ( 10 ) .
band intensity was scored by approximation using a 05 discrete scale , with 0 corresponding to no detection and 5 to highest presence ( relative abundance ) of a bacterial species .
species were identified based on a blast search of the human oral microbiome database ( 2 ) .
homings employs a probeseq program for species detection with modifications as previously described ( 15 ) .
amplification of the 16s rrna gene ( v3v4 region ) was followed by purification and processing using a modified next generation sequencing method as described by caporaso et al .
( 16 ) using a miseq ( illumina , inc . , san diego , ca ) .
probeseq sequence identification used rrna - based in silico probes in a blast program to determine the species taxa and frequency ( 15 ) .
species - specific 16s rrna - based oligonucleotide probes , many of which had originally been designed for homim , were used in a blast program , called probeseq for homings , to identify the frequency of oral bacterial targets .
probeseq loads the raw sequence files into a cell array and then loops through the array , one sequence at a time , looking for a
when a match is found , a running counter starts to give the total number of probe 
the probeseq process was iterative , that is , the sequences that had not been detected by a single species probe were subsequently processed against genus probes , which consist of two or more species within the genus .
all hits were accumulated by species / genus by patient , with a higher hit total interpreted as representing a higher presence of a given species .
modifications of the protocol include the following : dna was amplified directly in the initial pcr 
two rounds of pcr were used in the previous study ( 15 ) , and chimeric sequences were not removed from analyses for this study . however , in a separate analysis , chimeric sequences ranged from 10 to 15% of the total reads .
the relative proportions of detected taxa did not vary significantly ( data not shown ) .
homim intensity data ( 05 ) and homings hits data ( 0300,000 range ) were provided as excel spreadsheets for downstream statistical analysis .
homim in vitro signal intensity estimations and homings e - hybridization hits are not directly comparable . therefore , the data from both technologies were converted into relative proportions to allow semi - quantitative comparison of their outcomes .
common species total abundance per patient between the two technologies was determined based on species probes information . for the common species ,
wilcoxon signed - rank test was used to determine the statistical significance of differences found between homim and homings relative proportions at phylum , genus , and species levels , using a significance level of < 0.05 ( fdr [ false discovery rate ] adjusted p - value ) .
statistical analyses including outlier determination were performed using xlstat - pro ( version 2014.4.06 ) and/or sas enterprise guide ( version 6.1 ) .
patients ( n=96 ) were recruited from our hospital - based dental clinic as a subset of a previous clinical bacteremia study ( 8 , 12 ) .
patients were excluded from the study if they had fewer than 10 teeth , had taken a systemic antibiotic within 2 weeks prior to the study , or were immunocompromised .
patient demographics and clinical characteristics are summarized in table 1 
 ( 8) . as changes of clinical parameters were not the focus of this study , we kept the description here as basic . demographic and clinical characteristics of study cohort continuous values are mean ( sd ) , discrete values are number [ percentage ] .
calculus index : mean score for all tooth scores based on scale : 0=no calculus ; 1=supra - gingival ( not more than 1 mm ) ; 2=moderate supra - gingival and/or sub - gingival calculus ; and 3=abundance of supra - gingival and sub - gingival calculus .
gingival index : mean score of all tooth scores based on scale : 0=normal gingiva ; 1=mild inflammation , a slight change in color and edema , and no bleeding on probing ; 2=moderate inflammation , redness , edema , and bleeding on probing ; and 3=severe inflammation , marked redness and edema , ulcerations , and a tendency toward spontaneous bleeding .
plaque index : mean score of all tooth areas scores ( mesial , distal , facial , and lingual ) based on scale : 0=no plaque in the gingival area ; 1=no plaque visible to the unaided eye , but plaque is visible on the probe after being moved across the gingival crevice ; 2=gingival area covered with a thin to moderately thick layer of plaque visible to the naked eye ; and 3=heavy plaque accumulation and soft debris in the interdental area ( 8) .
dental plaque samples were collected at least 1 h before any dental manipulation , such as eating , tooth brushing , or tooth extraction .
they were acquired by scraping a dental scaler across the supra - and superficial sub - gingival aspect of 14 teeth , with the deepest probing depths , and pooled .
the sample was suspended in te buffer ( 50 mm tris hcl , 1 mm edta ph 7.6 ) , transferred on ice , and stored at 80c until furthzer analysis .
bacterial dna extraction was performed using a modification of qiaamp dna mini kit ( qiagen , valencia , ca ) , as described previously ( 12 ) and used for simultaneous analysis by homim and homings .
16s rrna gene sequences were polymerase chain reaction ( pcr ) amplified and labeled with cy3-dctp in a second nested pcr .
individual intensity signals were normalized by comparison to the average signals from universal 16s rrna gene probes ( 10 ) .
band intensity was scored by approximation using a 05 discrete scale , with 0 corresponding to no detection and 5 to highest presence ( relative abundance ) of a bacterial species .
species were identified based on a blast search of the human oral microbiome database ( 2 ) .
homings employs a probeseq program for species detection with modifications as previously described ( 15 ) .
amplification of the 16s rrna gene ( v3v4 region ) was followed by purification and processing using a modified next generation sequencing method as described by caporaso et al .
( 16 ) using a miseq ( illumina , inc . , san diego , ca ) .
probeseq sequence identification used rrna - based in silico probes in a blast program to determine the species taxa and frequency ( 15 ) .
species - specific 16s rrna - based oligonucleotide probes , many of which had originally been designed for homim , were used in a blast program , called probeseq for homings , to identify the frequency of oral bacterial targets .
probeseq loads the raw sequence files into a cell array and then loops through the array , one sequence at a time , looking for a
when a match is found , a running counter starts to give the total number of probe 
the probeseq process was iterative , that is , the sequences that had not been detected by a single species probe were subsequently processed against genus probes , which consist of two or more species within the genus .
all hits were accumulated by species / genus by patient , with a higher hit total interpreted as representing a higher presence of a given species .
modifications of the protocol include the following : dna was amplified directly in the initial pcr 
two rounds of pcr were used in the previous study ( 15 ) , and chimeric sequences were not removed from analyses for this study . however , in a separate analysis , chimeric sequences ranged from 10 to 15% of the total reads .
the relative proportions of detected taxa did not vary significantly ( data not shown ) .
homim intensity data ( 05 ) and homings hits data ( 0300,000 range ) were provided as excel spreadsheets for downstream statistical analysis .
homim in vitro signal intensity estimations and homings e - hybridization hits are not directly comparable . therefore , the data from both technologies were converted into relative proportions to allow semi - quantitative comparison of their outcomes .
common species total abundance per patient between the two technologies was determined based on species probes information . for the common species ,
wilcoxon signed - rank test was used to determine the statistical significance of differences found between homim and homings relative proportions at phylum , genus , and species levels , using a significance level of < 0.05 ( fdr [ false discovery rate ] adjusted p - value ) .
statistical analyses including outlier determination were performed using xlstat - pro ( version 2014.4.06 ) and/or sas enterprise guide ( version 6.1 ) .
homings and homim data were obtained from dental plaque samples of a patient cohort scheduled for single tooth extraction . demographics and gingival characteristics of these patients are shown in table 1 .
homings doubles the capacity for identification of bacterial species and increases the number of identifiable genera by nearly 85% compared to homim , based on use of species and genus probes counts ( table 2 ) . in our study of 96 pla 
 que samples , homings detected 489 species compared to 244 species by homim ( table 3 ) .
. a greater number of genera were also detected by homings ( i.e. 129 ) than homim ( i.e. 84 ) , corresponding to 53% increase ( table 3 ) .
detailed examination of the 129 genera revealed that 59 were represented by both species and genus probes , while 68 ( i.e. 105 species ) were represented by species probes alone .
unaccounted reads , that is , sequence reads that did not uniquely e - hybridize to species or genus probes , ranged from 4.5 to 36.7% [ mean% ( sd ) : 14% ( 4.9 ) ] of total sequences per sample and were over 20% for nine of the 96 samples ( data not shown ) .
species probes provide three categories of species identification : a unique species that is recognized by a single probe ( vast majority ) , a unique species that is recognized by 23 probes , and groups of 23 species that are recognized by a single probe . the number of single species that can be identified by each technology , after processing of multiple recognition occurrences , is presented .
species probes hits may be summed with genus probes hits to account for total genera hits .
the combination of species and genus probes can identify 159 genera , as a few species or genus probes do not have matching genus or species probes , respectively .
dental plaque microbiome composition by homim and homings 
  the data from patient cohort ( n=96 ) are presented .
( a ) the number of taxa ( species , genera , and phyla ) were determined based on the designation of species and/or genus probes . for the species identified in common by both homim and homings ( based on species probes only )
, there was no overall difference at species , genus , or phylum level , as determined by wilcoxon signed - rank test with fdr procedure ( fdr > 0.05 ) .
( b ) additional homings species and genus probes capacities are taken into account for comparison .
for each taxonomic group , the five taxa with the highest relative proportion are shown . at species level , relative proportions for porphyromonas gingivalis and dialister invisus show inverse trend between homim and homings . at genus level ,
, fusobacterium demonstrated the largest contribution by homings genus probes for genera undetected by homim .
relative proportions for fusobacterium and streptococcus show inverse trend between homim and homings , due to genus probes . at the phylum level ,
firmicutes and fusobacteria were the largest contributors by homings , with the latter benefiting most from genus probes hits . because homings detected double the number of species , full dataset statistical comparisons at any taxonomy level
would be deemed meaningless , as it would simply highlight the obvious . to assess the concordance between the two technologies , we adjusted the homings dataset to match those species contained in homim dataset to enable relative proportion of global comparisons at the phylum , genus , and species levels .
both homim and homings identified 198 species in common , corresponding to 74 genera and 10 phyla ( table 3 ) .
comparison of the relative proportions at three taxonomic levels ( e.g. species , genera , and phyla ) obtained by homim and homings showed no significant overall differences when fdr correction was applied for the multiple comparisons ( fdr>0.05 ) ( table 3 ) . in addition , we compared the total common species ( i.e. 198 spp . ) relative proportion per patient with regard to total homim intensities or homings hits for all 96 patients .
these results did not change when removing patient 26 ( pt26 ) , the most prominent outlier according to homings total species / genus relative proportions per patient ( p=0.805 ) ( fig .
pt26 had the highest number of total hits by homings ( 116,385 , i.e. 2.75% per total 96 patients ) compared to a relatively lower total intensity by homim ( i.e. 149 total intensity , i.e. 1.09% per total 96 patients ) .
further analysis showed that prevotella melaninogenica was responsible for this discrepancy compared to the other 197 common species in pt26 .
p. melaninogenica had 60,505 hits representing 52% of the total hits of 116,385 ( data not shown ) .
total taxa ( i.e. species common to both technologies ) relative proportion for a patient was calculated by dividing the sum of the intensity scores ( homim ) or hits ( homings ) of all bacterial taxa of the patient by the total number of these values for all 96 patients .
( a ) differences in relative taxa proportions are illustrated by patient to patient line chart ( homings [ blue ] ; homim [ red ] ) .
these differences were not statistically significant overall ( p=0.617 , wilcoxon signed - rank test ) .
( b ) grubbs test identified six patients as outliers , with patient 26 ( pt26 ) representing the most prominent outlier . removing pt26 from the analysis also showed that differences in taxa relative proportions were overall not statistically significant ( p=0.805 ) .
descriptive analysis of homings added taxa identification capacity is summarized in table 3 , and figs . 2 and 3 .
at the species level , porphyromonas gingivalis was the most represented species by homings ( table 3 ) .
the proportion of this species was nearly twice that of next most represented species , dialister invisus .
the opposite ratio was observed for the proportions of these two species detected by homim ( table 3 ) .
dental plaque bacterial genera composition by homings with added taxa identification capacity compared to homim . * homings microbiome profiling of dental plaque samples from a patient cohort ( n=96 ) shows a large increase ( > 100% ) in the detection of actinomyces , fusobacterium , leptotrichia , and prevotella genera compared to homim , likely due to additional genera probes .
relative proportions per genus were calculated based on total hits by homings or total intensity scores by homim per 96 patients .
dental plaque bacterial phyla composition by homings with added taxa identification capacity compared to homim . *
homings microbiome profiling of dental plaque samples from a 96 patients cohort shows a large shift in relative proportions for fusobacteria ( 3.8% homim vs. 29.4% homings ) and firmicutes ( 64.4% homim vs. 32.6% homings ) .
relative proportions per phylum were calculated based on total hits by homings or total intensity score by homim per 96 patients .
chloroflexi , gn02 and sr1 were omitted due to negligible representation . at the genus level ,
homings genus probes accounted for 38.7% of total hits ( table 3 ) . due to this
added capacity , larger relative proportions compared to homim were apparent for several genera including fusobacterium , prevotella , and leptotrichia ( table 3 , fig .
the highest relative proportion was observed for fusobacterium , that is , 23.4% ( including 2.1% species probes derived genera ) by homings , compared to 2.8% detected by homim ( table 3 ) .
the next four genera with the highest relative proportion detected by homings were streptococcus ( 14.1% including 6.2% species probes derived genera ) followed by prevotella , leptotrichia , and porphyromonas ( table 3 ) .
of these genera , streptococcus yielded similar proportions by homim ( 16.4% ) . at the phylum level , firmicutes represented twice the proportion by homim ( 64.4% ) compared to homings ( 32.6% ) ( table 3 , fig .
firmicutes and fusobacteria were represented in different proportions by homim alone ( 64.4 and 3.8% ) , whereas homings yielded nearly equal representation of these two phyla ( 32.6 and 29.4% ) .
additionally , fusobacteria were detected at a larger proportion by homings ( 29.4% ) compared to homim ( 3.8% ) ( table 3 , fig .
similarly , bacteroidetes and actinobacteria phyla proportions differed to a large extent using homim ( 11.8 and 3.5% ) and were in similar range ( 17 and 10.9% ) using homings . 

homings and homim data were obtained from dental plaque samples of a patient cohort scheduled for single tooth extraction . demographics and gingival characteristics of these patients are shown in table 1 .
homings doubles the capacity for identification of bacterial species and increases the number of identifiable genera by nearly 85% compared to homim , based on use of species and genus probes counts ( table 2 ) . in our study of 96 pla 
 que samples , homings detected 489 species compared to 244 species by homim ( table 3 ) .
. a greater number of genera were also detected by homings ( i.e. 129 ) than homim ( i.e. 84 ) , corresponding to 53% increase ( table 3 ) .
detailed examination of the 129 genera revealed that 59 were represented by both species and genus probes , while 68 ( i.e. 105 species ) were represented by species probes alone .
unaccounted reads , that is , sequence reads that did not uniquely e - hybridize to species or genus probes , ranged from 4.5 to 36.7% [ mean% ( sd ) : 14% ( 4.9 ) ] of total sequences per sample and were over 20% for nine of the 96 samples ( data not shown ) .
species probes provide three categories of species identification : a unique species that is recognized by a single probe ( vast majority ) , a unique species that is recognized by 23 probes , and groups of 23 species that are recognized by a single probe . the number of single species that can be identified by each technology , after processing of multiple recognition occurrences , is presented .
species probes hits may be summed with genus probes hits to account for total genera hits .
the combination of species and genus probes can identify 159 genera , as a few species or genus probes do not have matching genus or species probes , respectively .
dental plaque microbiome composition by homim and homings 
  the data from patient cohort ( n=96 ) are presented .
( a ) the number of taxa ( species , genera , and phyla ) were determined based on the designation of species and/or genus probes . for the species identified in common by both homim and homings ( based on species probes only )
, there was no overall difference at species , genus , or phylum level , as determined by wilcoxon signed - rank test with fdr procedure ( fdr > 0.05 ) .
( b ) additional homings species and genus probes capacities are taken into account for comparison .
for each taxonomic group , the five taxa with the highest relative proportion are shown . at species level , relative proportions for porphyromonas gingivalis and dialister invisus show inverse trend between homim and homings . at genus level ,
, fusobacterium demonstrated the largest contribution by homings genus probes for genera undetected by homim .
relative proportions for fusobacterium and streptococcus show inverse trend between homim and homings , due to genus probes . at the phylum level ,
firmicutes and fusobacteria were the largest contributors by homings , with the latter benefiting most from genus probes hits .
because homings detected double the number of species , full dataset statistical comparisons at any taxonomy level would be deemed meaningless , as it would simply highlight the obvious . to assess the concordance between the two technologies , we adjusted the homings dataset to match those species contained in homim dataset to enable relative proportion of global comparisons at the phylum , genus , and species levels .
both homim and homings identified 198 species in common , corresponding to 74 genera and 10 phyla ( table 3 ) .
comparison of the relative proportions at three taxonomic levels ( e.g. species , genera , and phyla ) obtained by homim and homings showed no significant overall differences when fdr correction was applied for the multiple comparisons ( fdr>0.05 ) ( table 3 ) .
in addition , we compared the total common species ( i.e. 198 spp . ) relative proportion per patient with regard to total homim intensities or homings hits for all 96 patients .
these results did not change when removing patient 26 ( pt26 ) , the most prominent outlier according to homings total species / genus relative proportions per patient ( p=0.805 ) ( fig .
pt26 had the highest number of total hits by homings ( 116,385 , i.e. 2.75% per total 96 patients ) compared to a relatively lower total intensity by homim ( i.e. 149 total intensity , i.e. 1.09% per total 96 patients ) .
further analysis showed that prevotella melaninogenica was responsible for this discrepancy compared to the other 197 common species in pt26 .
p. melaninogenica had 60,505 hits representing 52% of the total hits of 116,385 ( data not shown ) .
total taxa ( i.e. species common to both technologies ) relative proportion for a patient was calculated by dividing the sum of the intensity scores ( homim ) or hits ( homings ) of all bacterial taxa of the patient by the total number of these values for all 96 patients .
( a ) differences in relative taxa proportions are illustrated by patient to patient line chart ( homings [ blue ] ; homim [ red ] ) .
these differences were not statistically significant overall ( p=0.617 , wilcoxon signed - rank test ) .
( b ) grubbs test identified six patients as outliers , with patient 26 ( pt26 ) representing the most prominent outlier . removing pt26 from the analysis also showed that differences in taxa relative proportions were overall not statistically significant ( p=0.805 )
descriptive analysis of homings added taxa identification capacity is summarized in table 3 , and figs . 2 and 3 .
at the species level , porphyromonas gingivalis was the most represented species by homings ( table 3 ) .
the proportion of this species was nearly twice that of next most represented species , dialister invisus .
the opposite ratio was observed for the proportions of these two species detected by homim ( table 3 ) .
dental plaque bacterial genera composition by homings with added taxa identification capacity compared to homim . *
homings microbiome profiling of dental plaque samples from a patient cohort ( n=96 ) shows a large increase ( > 100% ) in the detection of actinomyces , fusobacterium , leptotrichia , and prevotella genera compared to homim , likely due to additional genera probes .
relative proportions per genus were calculated based on total hits by homings or total intensity scores by homim per 96 patients .
dental plaque bacterial phyla composition by homings with added taxa identification capacity compared to homim .
* homings microbiome profiling of dental plaque samples from a 96 patients cohort shows a large shift in relative proportions for fusobacteria ( 3.8% homim vs. 29.4% homings ) and firmicutes ( 64.4% homim vs. 32.6% homings ) .
relative proportions per phylum were calculated based on total hits by homings or total intensity score by homim per 96 patients .
chloroflexi , gn02 and sr1 were omitted due to negligible representation . at the genus level ,
homings genus probes accounted for 38.7% of total hits ( table 3 ) . due to this
added capacity , larger relative proportions compared to homim were apparent for several genera including fusobacterium , prevotella , and leptotrichia ( table 3 , fig .
the highest relative proportion was observed for fusobacterium , that is , 23.4% ( including 2.1% species probes derived genera ) by homings , compared to 2.8% detected by homim ( table 3 ) .
the next four genera with the highest relative proportion detected by homings were streptococcus ( 14.1% including 6.2% species probes derived genera ) followed by prevotella , leptotrichia , and porphyromonas ( table 3 ) .
of these genera , streptococcus yielded similar proportions by homim ( 16.4% ) . at the phylum level , firmicutes represented twice the proportion by homim ( 64.4% ) compared to homings ( 32.6% ) ( table 3 , fig .
3 ) . firmicutes and fusobacteria were represented in different proportions by homim alone ( 64.4 and 3.8% ) , whereas homings yielded nearly equal representation of these two phyla ( 32.6 and 29.4% ) .
additionally , fusobacteria were detected at a larger proportion by homings ( 29.4% ) compared to homim ( 3.8% ) ( table 3 , fig .
similarly , bacteroidetes and actinobacteria phyla proportions differed to a large extent using homim ( 11.8 and 3.5% ) and were in similar range ( 17 and 10.9% ) using homings . 

identification of bacterial species from various sites in the mouth and quantification of the relative abundance of each species is a daunting task , given the wide diversity of species known to inhabit the human oral cavity .
both homim and homings provide semi - quantitative identification of oral microbiome bacterial species . using the same dental plaque samples in this study , homings technology expanded the detection of the number of species ( via species - level probes ) by 100% and the number of genera ( via species probes and genus probes ) by nearly 85% .
this represents a significant improvement toward achieving full knowledge of oral bacterial composition at the species level .
this capability gives recognition to the fact that bacterial species do not all provide equal unique e - hybridization potential based on the degree of conservation of the v3v4 region of the 16s rrna gene .
examples of highly conserved species that are not effectively uniquely e - hybridized to species - level probes are found in several genera including fusobacterium and streptococcus . by using an iterative process to combine species with genus probe e - hybridization events , homings technology maximizes reads identification .
however , for our dental plaque dataset , there was an average of 14% unaccounted reads per patient .
these correspond to sequences which either do not e - hybridize to a target probe or e - hybridize to multiple target probes .
these sequences provide opportunities for further species probe refinement and new probe development in order to identify all bacterial species present in oral samples to the lowest detection limit possible .
identification of species - level information is preferable , but in the absence of this , genus level information is useful and informative .
in fact , genus probes altogether accounted for nearly 40% of total hits in this study .
therefore , for genera that have species probes but no genus probes , it is unclear whether there are species remaining unaccounted for within the genus .
in addition , within the bulk of genus probes total hits , fusobacterium represented ~20% of hits , followed by , but to a significantly smaller extent , streptococcus ( ~8% ) .
therefore , the largest contribution by homings with the addition of genus probes is the added capacity of detecting more fusobacterium compared to homim .
based on analyses of the samples tested , it seems clear that some key taxa of fusobacterium , leptotrichia , and/or actinomyces are not differentiated at the species level .
additionally , similar proportions for firmicutes with fusobacteria and bacteroidetes with actinobacteria were determined by homings , but not by homim , as probes for many of 
 these taxa were difficult to construct within proper hybridization parameters , for example , gc content and tm .
nevertheless , homim phylum level data were in overall agreement with the literature ( 4 ) , namely , firmicutes was the most predominant phylum .
results obtained by homings suggest a more balanced representation of several phyla of dental plaque . however , they might only be representative of the patient population in need for tooth extraction used in our study .
therefore , the results may not be extrapolated to the general population with a better oral hygiene and less dental disease , which clearly may impact the composition of the dental plaque microbiome .
in addition , the introduction of unique e - hybridization events ( hit counting ) to determine the relative abundance of species and genera is a more precise semi - quantitative process as compared to homim .
the fluorescence - based quantification employed in homim provides only a rough estimate of relative proportions on a narrow discrete scale basis ( 05 ) .
in contrast , homings in silico matching of target sequence to probe must be exact for a hit to be counted , resulting in no ambiguity of presence or absence of a species .
thus , a low number of hits determined by homings would most likely correspond to background intensity or no detection by homim .
taking into account the fact that there is no practical limitation to the number of hits that can be counted , hit counting intrinsically provides a more precise and comprehensive representation of the species relative abundance .
these fundamental changes make direct comparisons of homings semi - quantitative data with those of homim difficult .
a fair comparison can not be achieved because there is no method to convert in vitro hybridization intensities accurately for each species into e - hybridization hits . nonetheless , in the present study , a comparison of relative proportions limited to those species found in both homim and homings demonstrated concordance between the two technologies in that there were no statistically significant differences noted in the overall results at the phylum , genus , or species taxonomic levels .
concordance between the two technologies was also found regarding the total species relative proportion by patient .
in essence , homings partially confirmed homim data obtained from the same dental plaque sample collection , while differences largely out of range might have been anticipated . in this respect ,
previous studies that have used homim have been helpful to the extent that differences between disease and control groups can be understood on a global microbial community at the species level to the limit of technology accuracy . in conclusion , the determination of bacterial species composition in the oral cavity will likely move forward in a significant way with the adoption of homings . assuming that further improvements of homings or other bioinformatic technology would enable detection of all the species present in various oral sites , true quantification may be achieved based on the calibration of bacterial dna input and knowledge of the bacterial genome sizes for the species detected .
refinement of homings will provide a better identification tool for the oral microbiome and will be beneficial to the understanding of distant site infections originating from the oral cavity , such as ie and pjis .
 backgroundover 700 bacterial species reside in human oral cavity , many of which are associated with local or distant site infections . 
 extensive characterization of the oral microbiome depends on the technologies used to determine the presence and proportions of specific bacterial species in various oral sites.objectivethe objective of this study was to compare the microbial composition of dental plaque at baseline using human oral microbe identification microarray ( homim ) and human oral microbe identification using next generation sequencing ( homings ) technologies , which are based on 16s rrna.methodsdental plaque samples were collected from 96 patients at baseline prior to a dental procedure involving manipulation of gingival tissues . 
 the samples were surveyed for 293 and 597 oral bacterial species via homim and homings , respectively , based on 16s rrna gene sequences . 
 we determined the concordance between the two technologies for common species . 
 genus level analysis was performed using homings - specific genus identification capabilities.resultshomings detected twice the number of species in the same dental plaque samples compared to homim . for the species detected by both homim and homings , there was no difference in relative proportions of overall bacterial composition at the species , genus or phylum levels . additionally , there was no difference in relative proportion for total species per patient between the two technologies.conclusionhomings significantly expanded oral bacterial species identification compared to homim . 
 the genus and species probes , combined in homings , provided a more comprehensive representation of oral bacterial community , critical for future characterization of oral microbes in distant site infections . 
Materials and methods
Study population
Sample collection and DNA extraction
HOMIM and HOMI
Statistical analysis
Results
HOMIM and HOMI
Analysis of the common taxa detected by both HOMIM and HOMI
Analysis of the HOMI
Discussion
Author contributions
Conflict of interest and funding