the burgundy   perinatal network was created in 1992 to improve the quality of 
 perinatal care in burgundy , 
 a french region with 1 800 000 inhabitants and 18 500 births annually . 

the medical 
 steering committee , made up of medical representatives of each hospital ( one 
 paediatrician and one obstetrician for each ) has progressively reorganized 
 perinatal care in the region . in particular ,
medical conventions between hospitals 
 were signed to improve coordination of admissions , transfers of patients , and 
 the use of technical facilities . moreover , a half - yearly systematic analysis of 
 perinatal deaths was initiated .
the 
 impact of actions promoted in the perinatal network has been assessed yearly 
 since 1998 .
a 
 multidisciplinary working group previously chose and precisely defined specific 
 indicators ( 25 for each mother and 17 for each newborn ) [ 1 , 2 ] . these indicators 
 correspond to maternal medical history , psychosocial risk factors , maternal and 
 neonatal medical data such as hospitalisations during pregnancy , delivery 
 method , postpartum period , and the different hospitalisations of the newborns .
most 
 of these items could be obtained from the mandatory discharge abstracts for 
 each hospitalised patient within the programme de mdicalisation du systme 
 dinformation ( pmsi ) .
the pmsi collection system was used as it is compulsory 
 in both public and private hospitals in france .
these data are rendered anonymous 
 before being sent from each hospital to the committee in charge of the 
 assessment of the perinatal network 's performance ( regional audit committee ) .
optimal 
 assessment of perinatal care obviously requires effective linkage between 
 maternal and neonatal data .
indeed , in order to study the mechanisms of 
 neonatal diseases it is essential to link ( 1 ) the abstracts of a mother to 
 those of her newborn even if they were not cared for in the same hospital and ( 2 ) 
 all discharge abstracts for the same patient ( mother and/or newborns ) , who may 
 have several discharge abstracts from different hospitals . in our case , this 
 happens when an antenatal or postnatal transfer from one hospital to another is 
 decided , according to the written rules for good medical practice in the 
 perinatal network .
this 
 study intends to assess the performance of the file - linkage process of maternal 
 and neonatal data used for the evaluation of the burgundy perinatal network .
all 
 deliveries and births , whether live   or not , are included in the perinatal 
 network if the gestational age is at least 22 weeks 
 and/or if the birth weight is greater than 500 g. for the purpose of this 
 study , we took into account the population included from 1998 , the year data 
 collection began , to 2001 . 
  in 1998 , only nine hospitals participated in the data 
 collection , whereas in 2001 all of the 18 hospitals involved in the regional 
 perinatal care network were involved , thus providing data for 100% of the 18 500 annual births .
discharge 
 abstracts for all mothers ( social and medical data about gestation , childbirth 
 and the postnatal period ) and all newborns ( hospitalisations in maternity 
 hospitals , paediatric wards , neonatal units , and neonatal intensive care units ) 
 collected within the pmsi were provided by each hospital in burgundy .
the collection of discharge 
 abstracts is mandatory and does not require the agreement of the patient .
the 
 usual data collection system of the pmsi was expanded to include the collection 
 of birth weight and gestational age .
moreover , six identification items ( maiden 
 names , first names and birth dates of mothers , first names and birth dates of newborns , 
 zip codes of the main residence of mothers ) were recorded for both mothers and newborns 
 to allow linkage between their discharge abstracts .
the directive 95/46/ce of the european parliament and 
 of the council of 24 october 1995 on the protection of individuals with regard to the processing of personal data and on the free movement of such data and the act n78 - 17 of 6 
 january 1978 on data processing , data files and individual 
 liberties ( amended by the act of 6 august 2004 relating to 
 the protection of individuals with regard to the processing of 
 personal data ) are the european and french legislations on 
 data privacy and security . following these recommendations , 

the data were rendered anonymous in each hospital using 3 
 anonymat software [ 35 ] .
the principle of this   software based on the standard hash algorithm is to 
 ensure the irreversible transformation of independent fields , which prevents 
 the identification of an individual .
the aim is to obtain a strictly anonymous 
 code , but always the same one for a given couple of a mother and her newborn(s ) . 

the use of anonymat software has been authorized by the french department for 
 information system security ( scssi ) , and in 1998 the french data protection 
 commission ( commission nationale de l'information 
 et des liberts  cnil ) specifically 
 authorized the management of perinatal data in burgundy .
the collection of the data does 
 not require the specific consent of the patient , who only has to be informed 
 that the information contained in discharge abstracts will be used for 
 epidemiological purposes and not only for budgetary purposes .
an information 
 leaflet explaining the use of the data is given to the patient on admission to 
 the hospital . in 
 order to reduce the impact of typing errors ,
the principle is to 
 transform the spelling of names according to phonetic rules . among the 
 different treatments ,
soundex is most widely used . however , in the perinatal 
 network , a treatment specifically adapted to the particularities of the french 
 language was used .
once 
 rendered anonymous , the data were transmitted to the regional audit committee 
 located in the university hospital of dijon ( france ) and included in a regional 
 database ( cf .
the first part of the linkage process is to gather all 
 discharge abstracts concerning the same patient ( mother or newborns ) from the 
 same or different hospitals .
the second part is devoted to linking the 
 abstracts of a mother to those of her newborn even if they were not cared for 
 in the same hospital . in order to respect the confidentiality of medical files , 

linkage is carried out on files that are rendered anonymous and not directly on 
 nominative data .
technically , there is no difference between using original 
 nominative data or one - way hash - coded data for record linkage , as the 
 correspondence between these data is almost one - to - one ( low collision rate ) . in 
 fact
, the spelling processing used in the anonymity procedure reduces the 
 impact of typing errors and increases the efficiency of the linkage .
however , 
 two types of linkage errors are of concern : erroneous links of notifications 
 ( information ) from two distinct patients , also called homonym errors , and 
 failure to link multiple notifications ( information ) on the same patient , also 
 called synonym errors .
these 
 errors would be reduced if we could use the french identification number which 
 is unique for each individual .
however , this number is not recorded by 
 hospitals , which only use the social security number ( ssn ) , which is not unique 
 for each individual as it is assigned to each insured person , who may register 
 his family under the same ssn .
thus , the ssn of a woman may change , for 
 example , after her marriage . moreover ,
communication of the ssn is not allowed 
 by french legislation except for the transmission of information from 
 private - sector hospitals to french health - insurance companies .
therefore , 
 we have developed a record linkage system which is based on the method proposed 
 by jaro   and takes into account the 6 identification variables . in 
 this procedure , like in other probabilistic methods [ 815 ] ,
a weight is 
 associated with each variable according to the reliability of the information 
 provided .
the weight given to two identical dates of birth is then higher than 
 that given to two identical zip codes , as date of birth is more discriminating 
 than zip code .
it is then possible to compute the weight corresponding to each 
 pair of records by summing the positive weights obtained for concordant 
 variables and negative weights for discordant variables .
two threshold values 
 can then be computed , from which three sets of possible decisions can be 
 determined as follows : ( 1 ) the pair is matched ; ( 2 ) no determination is made ; ( 3 ) 
 the pair is not matched . in case 2 , no determination , other information is 
 explored .
it 
 was assumed that a link between a mother and a noncorresponding newborn was impossible 
 in the case of perfect agreement between two records on the 6 identification 
 items .
indeed , it is highly unlikely that in the same maternity hospital , two 
 women having the same zip code , the same maiden name , the same first name , the 
 same birth date would give birth on the same day to babies with the same first 
 name . additionally , the risk for a homonym error was very low ( 10 ) with the standard hash algorithm . in 
 a perinatal network , it is obvious that every newborn has a mother and vice 
 versa .
when a mother abandons her infant at birth and has the right to remain 
 anonymous , the maiden name , the first name , and the date of birth of the mother 
 were filled with 
 random numbers and thus rendered anonymous in the hospital file before hash 
 coding , both in the mother 's and in the newborn 's files . as the linkage items for 
 the mother and
her newborn are the same ( filled in with the same numbers ) , 
 linkage is possible .
so , 
 the fact that no link was found between a newborn and a mother would indicate 
 either a linkage error or the lack of the mother 's record .
linkage errors were 
 identified by again using the linkage method on the basis of only five items or 
 even four . to verify these potential links ,
each hospital was asked to verify 
 the identification items of the records corresponding to the given anonymous 
 numbers .
the corrected data were rendered anonymous before being returned from 
 the hospitals to the regional database .
finally , 
 the linkage was only performed on the basis of all 6 identification items in 
 the final database . before 
 linkage ,
the exhaustiveness of both medical items and linkage items was monitored as follows : 
 ( 1 ) exhaustiveness for the 6 linkage items was verified for each discharge 
 abstract ; ( 2 ) exhaustiveness for gestational age and birth weight was verified 
 for each neonatal discharge abstract .
after 
 linkage , the exhaustiveness for the number of mothers and newborns registered 
 in the regional database was assessed from hand - written notebooks which are 
 used in each hospital for the registration of births and/or admissions of sick 
 newborns to units caring for these infants .
the law requires these hand - written 
 notebooks to be completed . regarding 
 data quality
, computerized procedures have also been developed to reveal 
 discrepancies between medical data or between dates of exit and admission for 
 successive hospitalisations .
all erroneous data were then corrected in the nominative files 
 in each hospital , before being rendered anonymous and sent back to the regional 
 database . as a consequence , after validation , the number of mothers and 
 newborns agreed with hand - written notebooks , and the medical or administrative 
 inconsistencies between discharge abstracts were corrected .
we then considered 
 data after validation as a gold standard for the assessment of the linkage 
 procedure ( on data before validation ) .
the exhaustiveness of data collection and the 
 linkage rate have improved with time . during 
 validation after linkage , the number of mothers and newborns registered in the 
 regional database was assessed from the hand - written notebooks which are used 
 in each hospital for the registration of births and/or admissions of sick 
 newborns to units caring for these infants . at the beginning of the collection 
 in 1998 or 1999 , we detected some discrepancies .
( 1 ) exhaustiveness of discharge abstractsin 1998 , 9 hospitals were involved in the collection of discharge abstracts . 

abstracts for 84.1% and 99.1% of all eligible mothers were retrieved in the 
 regional database , respectively , before and after the validation procedure ; for 
 newborns , the percentages were 100% and 98.7% .
overestimation of the 
 hospitalisation rate in some neonatal files in 1998 was explained by undue 
 inclusion of hospitalisation beyond the neonatal period.from 
 2001 to 2005 , 18 hospitals were involved in the collection of discharge 
 abstracts and the overall exhaustiveness for both mothers and newborns reached 
 99.9% after the validation procedure .
the exhaustiveness of discharge abstracts 
 was 100% but some mothers may not have been hospitalised if the delivery 
 occurred at home . in 1998 , 9 hospitals were involved in the collection of discharge abstracts . 

abstracts for 84.1% and 99.1% of all eligible mothers were retrieved in the 
 regional database , respectively , before and after the validation procedure ; for 
 newborns , the percentages were 100% and 98.7% .
overestimation of the 
 hospitalisation rate in some neonatal files in 1998 was explained by undue 
 inclusion of hospitalisation beyond the neonatal period . from 
 2001 to 2005 , 18 hospitals were involved in the collection of discharge 
 abstracts and the overall exhaustiveness for both mothers and newborns reached 
 99.9% after the validation procedure .
the exhaustiveness of discharge abstracts 
 was 100% but some mothers may not have been hospitalised if the delivery 
 occurred at home .
( 2 ) exhaustiveness of the data in the discharge abstractsin 
 1998 , the six items used for the linkage procedure were recorded in 80% of 
 discharge abstracts before validation and in 99% after validation . from 2001 to 
 2005 , the exhaustiveness of these items was between 91.9% and 98.5% before and 100% after validation . in 
 1998 , the six items used for the linkage procedure were recorded in 80% of 
 discharge abstracts before validation and in 99% after validation . from 2001 to 
 2005 , the exhaustiveness of these items was between 91.9% and 98.5% before and 100% after validation .
( 3 ) linkage assessmentbefore 
 validation , the percentage of newborns linked to their corresponding mothers on 
 the basis of the 6 identification items was 71% in 1998 , 92.9% in 2001 , and 
 93.4% in 2005.different 
 types of errors were found during the validation procedure after linkage .
the 
 most frequent one corresponded to errors in spelling surnames and first names 
 leading to phonetic changes that were not subsequently corrected by the 
 spelling processing included in the anonymat software .
the inversion of the 
 married name , and the maiden name , during data collection was also a source of 
 linkage failure , and was corrected through the validation procedure .
after 
 validation , 99.98% of newborns were linked to their mothers whatever the year 
 concerned .
only newborns who were transferred from a hospital of the burgundy 
 region to a hospital of another region were missed ( 0.2%).the 
 results regarding the assessment of the linkage procedure , using validated data 
 as a gold standard , are presented in table 1 , for each year.sensitivity 
 is most often higher than 90% ( 97.1% in 2006 ) .
the year 2004 may be considered as an outlier : several 
 hospitals had to change their software and faced many difficulties in 
 collecting data .
specificity is nearly always higher than 85% , except in 2006 
 ( 77.7%).the 
 estimation of the false positive and false negative rates , given in table 1 , 
 also indicates an unexpected higher rate of false positives in 2006 ( 5.3% ) .
the 
 analysis of the false positives is presented in table 2 , which provides , in 
 case of false positives , the percentage of missing data for each identification 
 item .
we can observe that the main reason for false positives appears to be 
 related to missing data ; this was particularly the case in 2006 . among false 
 positives ,
85.11% were due to perfect concordance between the record of the 
 baby and the record of the mother on the birth dates of the mother and on the 
 zip codes of the main residence of the mother , while the other items were 
 nearly all missing.of 
 course , in these cases , agreement between the two records on the 6 
 identification items can not be considered perfect .
in fact , in case of missing 
 data on an identification item , the two records are considered discordant on 
 this item.for 
 false negatives , the same analysis is presented in table 3 . here again , missing 
 data is the main cause . in particular , because of missing data on the maiden 
 and first names of the mother , the two records were often considered discordant 
 on these items , which prevented linkage of these records .
hopefully these 
 errors have been corrected thanks to the validation procedure . before 
 validation ,
the percentage of newborns linked to their corresponding mothers on 
 the basis of the 6 identification items was 71% in 1998 , 92.9% in 2001 , and 
 93.4% in 2005 .
the 
 most frequent one corresponded to errors in spelling surnames and first names 
 leading to phonetic changes that were not subsequently corrected by the 
 spelling processing included in the anonymat software .
the inversion of the 
 married name , and the maiden name , during data collection was also a source of 
 linkage failure , and was corrected through the validation procedure .
after 
 validation , 99.98% of newborns were linked to their mothers whatever the year 
 concerned .
only newborns who were transferred from a hospital of the burgundy 
 region to a hospital of another region were missed ( 0.2% ) .
the 
 results regarding the assessment of the linkage procedure , using validated data 
 as a gold standard , are presented in table 1 , for each year .
the year 2004 may be considered as an outlier : several 
 hospitals had to change their software and faced many difficulties in 
 collecting data .
specificity is nearly always higher than 85% , except in 2006 
 ( 77.7% ) .
the 
 estimation of the false positive and false negative rates , given in table 1 , 
 also indicates an unexpected higher rate of false positives in 2006 ( 5.3% ) .
the 
 analysis of the false positives is presented in table 2 , which provides , in 
 case of false positives , the percentage of missing data for each identification 
 item .
we can observe that the main reason for false positives appears to be 
 related to missing data ; this was particularly the case in 2006 . among false 
 positives ,
85.11% were due to perfect concordance between the record of the 
 baby and the record of the mother on the birth dates of the mother and on the 
 zip codes of the main residence of the mother , while the other items were 
 nearly all missing .
of 
 course , in these cases , agreement between the two records on the 6 
 identification items can not be considered perfect .
in fact , in case of missing 
 data on an identification item , the two records are considered discordant on 
 this item . for 
 false negatives ,
the same analysis is presented in table 3 . here again , missing 
 data is the main cause . in particular , because of missing data on the maiden 
 and first names of the mother , the two records were often considered discordant 
 on these items , which prevented linkage of these records . hopefully these 
 errors have been corrected thanks to the validation procedure .
optimal 
 assessment of perinatal care needs a linkage procedure between successive files 
 for the same patient and between files for the mothers and their corresponding newborn(s ) . 

the latter linkage was found to be essential in the assessment of the postnatal 
 consequences of antenatal risk factors and maternal diseases .
for instance , in 
 1999 , one maternity hospital showed significantly higher rates of both 
 caesarean section and neonatal hospitalisation compared with regional and 
 national rates .
the linkage procedure revealed that the excess in neonatal 
 hospitalisations was related to an excess in the use of caesarean section in a 
 population of mothers similar to that admitted to other maternity hospitals in burgundy .
this finding was 
 a strong argument for reorganising perinatal care leading to a significant 
 decrease in the use of caesarean section in this hospital .
the 
 fact that each mother corresponded to a newborn and vice versa was particularly 
 helpful in testing the linkage procedure .
indeed , this study demonstrated that 
 coupling the direct linkage of anonymous data files with the validation 
 procedure that takes into account the potential links revealed by the 
 statistical procedure generated very satisfactory results on a regional scale .
this 
 linkage method could also be used to link the siblings ( having the same 
 mother ) .
the linkage of 
 full brothers or sisters   by sorting the dates of birth of all the individuals with 
 the same parents , half brothers and sisters   by sorting the dates of birth of all the individuals 
 with the same father or the same mother , 
 would require the implementation of a new identifier , such as the family - based 
 identifier ( also including the names and date of birth of the father ) [ 17 , 18 ] .
full brothers or sisters   by sorting the dates of birth of all the individuals with 
 the same parents , half brothers and sisters   by sorting the dates of birth of all the individuals 
 with the same father or the same mother , the 
 assessment method used in burgundy relied on the pmsi system .
the main advantages of using the pmsi information 
 system were that this data collection system is mandatory in all french 
 hospitals and that the classification used in this program ( i.e. , the 
 international disease classification10th revision ) contained most of the items 
 necessary for an audit of the perinatal network .
moreover , the number of 
 additional items included was limited . although pmsi was not designed for the 
 assessment of care networks , using this information system made it unnecessary 
 to set up another data collection system in each participating hospital and 
 thus avoided duplication of work .
however , 
 the collection of additional data and the need to extract items from pmsi required 
 extension of the existing software in each hospital .
moreover , these changes involved 
 several companies that were more or less interested in modifying their 
 software .
moreover , health 
 professionals and a computer engineer were needed for validation procedures , 
 management of the regional database , statistical analyses , transmitting 
 regional data to the medical steering committee , drafting reports , and helping 
 the hospitals to improve item recording .
our 
 results showed that satisfactory collection of the linkage items was more 
 difficult to obtain than was the collection of medical items .
this is easily 
 explained by the fact that physicians are less motivated to collect 
 administrative data .
therefore , the validation procedure for the identification 
 items was made a cornerstone in the quality process .
the 
 results regarding the assessment of the linkage procedure revealed that 
 sensitivity and specificity are quite high ( resp .
the decrease in sensitivity in 2004 is related to software changes in several 
 hospitals .
the decrease in specificity in 2006 , as well as the overall false 
 positive rate among the whole period , seemed to be the consequence of missing 
 data .
these observations led us to propose harmonization of the procedures 
 ( software and rules for collecting data ) in all hospitals .
this initiative , 
 called the extranat project , is described in the next section .
the burgundy perinatal 
 network was created in 1992 because of the high perinatal morbidity and 
 mortality in france ( 7th highest 
 among ocde countries ) notably in burgundy ( 14th 
 highest in france ) . since 1995
a hierarchal interestablishment 
 network of all of the maternity clinics ( 1 type iii maternity clinic , 2 type ii 
 maternity clinics , 8 type i maternity clinics , and 4 local perinatal care 
 centers ) .
the missions of each establishment and the criteria and procedures 
 for the transfer of the mother ( before or after delivery ) and the newborn have 
 been defined.a common paper - based record document.an original and exhaustive evaluation system , 
 based on the pmsi , for all of the 18 000 annual pregnancies , with anonymization of data in each 
 establishment after discharge , and linkage between the mother and her infant . 
 
a hierarchal interestablishment 
 network of all of the maternity clinics ( 1 type iii maternity clinic , 2 type ii 
 maternity clinics , 8 type i maternity clinics , and 4 local perinatal care 
 centers ) .
the missions of each establishment and the criteria and procedures 
 for the transfer of the mother ( before or after delivery ) and the newborn have 
 been defined . a common paper - based record document .
an original and exhaustive evaluation system , 
 based on the pmsi , for all of the 18 000 annual pregnancies , with anonymization of data in each 
 establishment after discharge , and linkage between the mother and her infant .
thanks to the 
 combination of these three elements and to the perseverance of those involved 
 in perinatal care and the burgundy dim , since 1999 , our region has climbed to 
 be among the top three in france 
  with regard to low maternal and perinatal mortality and morbidity . however , 
 though retrospective analysis of records on perinatal mortality showed a 
 considerable improvement in the quality of intra- and interhospital care 
 between 1996 and 2003 ( in - utero transfers for extremely premature births , 
 high - risk patients , antenatal corticotherapy , pediatric care at the maternity 
 clinic ) , it also revealed a significant deterioration in preclinic conditions , showing 
 the need to set up an 
tool for all of those involved in perinatal 
 care ( gps , specialists , midwives ) , whether they are based in public hospitals , 
 in private practice or in mother and child care centres ( protection maternelle 
 et infantile ) .
project , a computer system to facilitate 
 the communication ( between professionals),the training and the evaluation of 
 professional practices ( discussions about protocols),the implementation of shared interactive 
 computerized medical records , with the medical and organizational references of 
 the network . 
 
the communication ( between professionals ) , the training and the evaluation of 
 professional practices ( discussions about protocols ) , the implementation of shared interactive 
 computerized medical records , with the medical and organizational references of 
 the network . thanks to extranat , 
 it will be possible to have real - time communication , and regular meetings in the 
 form of videoconferences ( telemedicine ) to study problem cases .
the telemedicine 
 system is already up and running and extranat is currently being set up .
the aim of 
 this project is to fulfill the three missions of the perinatal network as follows : 
				 

								 improvement of the quality of care by enabling the health - care professional to input 
 initial medical data ( no longer final data at discharge from the 
 establishment ) .
this is a criterion in the improvement in information 
 collection and in the sharing of medical data with complementary specialists 
 ( specialists in women 's health , specialists in neonatal health for infants that 
 may be transferred to other medical departments or other establishments),evaluation of professional practices , 
 which requires identification and anonymization of the professionals concerned , evaluation of the medical and organizational 
 aspects of the burgundy perinatal network 
 ( anonymized database with mother - child linkage ) , already operational and presented 
 in this article . 
 
								
improvement of the quality of care by enabling the health - care professional to input 
 initial medical data ( no longer final data at discharge from the 
 establishment ) .
this is a criterion in the improvement in information 
 collection and in the sharing of medical data with complementary specialists 
 ( specialists in women 's health , specialists in neonatal health for infants that 
 may be transferred to other medical departments or other establishments ) , evaluation of professional practices , 
 which requires identification and anonymization of the professionals concerned , evaluation of the medical and organizational 
 aspects of the burgundy perinatal network 
 ( anonymized database with mother - child linkage ) , already operational and presented 
 in this article .
it is 
 therefore essential for the new extranat system to use the same patient 
 identification and anonymization system ( mothers , newborns , fathers ) as the perinatal 
 network , so as to 
 provide access ( limited and 
 controlled ) to medical data for professionals who are treating the mother or newborn 
 ( data identified at the physician 's practice and/or the medical establishment),respect the anonymization 
 requirements for data for the patients ( as requested by the cnil ) , and for the 
 professionals , in the regional data base . 
 
provide access ( limited and 
 controlled ) to medical data for professionals who are treating the mother or newborn 
 ( data identified at the physician 's practice and/or the medical establishment ) , respect the anonymization 
 requirements for data for the patients ( as requested by the cnil ) , and for the 
 professionals , in the regional data base .
it is possible to set up a continuous and exhaustive recording system for linked 
 perinatal data to assess the quality of care on a regional scale .
a pre - existing 
 database like pmsi may be valuable , but may have to be extended if necessary . 

the linkage of anonymous files may greatly enhance the accuracy of the 
 assessment procedure .
these principles of assessment of a perinatal network 
 could be extended to other medical domains .
 to assess the burgundy perinatal network ( 18 obstetrical units ; 18 500 births per year ) , discharge abstracts and additional data were collected for all mothers and newborns . in accordance with french law , data were rendered anonymous before statistical analysis , and were linked to patients using a specific procedure . 
 this procedure allowed data concerning each mother to be linked to those for her newborn(s ) . 
 this study showed that all mothers and newborns were included in the regional database ; the data for all mothers were linked to those for their infant(s ) in all cases . 
 additional data ( gestational age ) were obtained for 99.9% of newborns . 
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
5. Conclusion