the endothelial blood  brain barrier ( bbb ) , composed of the highly specialized central nervous system ( cns ) microvascular endothelial cells , and the epithelial blood  cerebrospinal fluid barrier ( bcsfb ) , composed of the choroid plexus epithelial cells , protect the cns from the constantly changing milieu in the bloodstream as well as infections and toxins , and are thus essential for maintaining cns homeostasis ( fig .
1 ) . work on how these barriers prevent paracellular diffusion of harmful elements into the cns usually focuses on characterization of the highly complex tight junctions ( tjs ) of the bbb , which resemble epithelial tjs ( kniesel et al . , 1994 ; liebner et al . , 2000b ) , and the unique parallel running tj strands of the bcsfb ( engelhardt et al . , 2001 ; wolburg and paulus , 2010 )
yet , formation of tjs generally requires the existence of adherens junctions ( ajs ) , and central to the organization of dynamic junctions is the continuous crosstalk between components of ajs and tjs , a fact often neglected by researchers studying the bbb and the bcsfb .
ajs do certainly exist in these barriers and are established between neighboring cells by the homophilic interaction between the transmembrane proteins vascular endothelial cadherin ( ve - cadherin ) and epithelial cadherin ( e - cadherin ) in cns endothelial and choroid plexus epithelial cells , respectively ( vorbrodt and dobrogowska , 2003 ) .
intercellular junctions between cns microvascular endothelial cells forming the bbb and between choroid plexus epithelial cells forming the bcsfb .
( a ) schematic of the localization of the brain barriers in parenchymal microvessels ( b ) and the choroid plexus in the ventricles ( c ) in a coronal brain section .
( b ) localization ( top ) and molecular composition ( bottom ) of endothelial bbb ajs and tjs .
ajs at the bbb are established by the homophilic interaction of cis dimers of transmembrane ve - cadherin in between adjacent endothelial cells .
the cytoplasmic tail of ve - cadherin binds the armadillo family proteins p120- and -catenin , which , via interaction with -catenin and afadin ( af-6 ) , mediate the link to the actin cytoskeleton .
nectins are transmembrane proteins belonging to the immunoglobulin ( ig ) superfamily that form homodimers in cis and contribute to aj formation by adhering to nectins on the adjacent cell and binding af-6 via their cytoplasmic tail , which connects nectin with the actin cytoskeleton .
ve - cadherin can also bind to plakoglobin ( -catenin ) instead of to -catenin and thus connect endothelial ajs to the intermediate filaments in addition to the actin cytoskeleton .
although drawn separately in this figure , tj strands that encircle the entire circumference of the cns endothelial cells are intermingled with ajs .
the transmembrane tj proteins found to be localized in cns endothelium are occludin , claudin-3 , claudin-5 , and claudin-12 as well as the junctional adhesion molecules ( jam ) a , b , and c. both claudins and jams mediate homophilic and heterophilic cis and trans interactions within their family , thus sealing the paracellular cleft between the adjacent endothelial cells . with the exception of claudin-12 ,
the transmembrane tj proteins carry a pdz - binding motif in their carboxy terminus , through which they bind the scaffolding proteins zo-1 and zo-2 and , in the case of jams , also af-6 .
zo-1 has also been shown to bind to -catenin and f - actin and thus regulate ajs .
additional molecules found to be present in bbb cell - to - cell contacts are the ig supergene family members esam , pecam-1 , and cd99 .
linkage of tj proteins to cell polarity complexes are omitted from this figure for reasons of simplicity .
( c ) localization ( top ) and molecular composition ( bottom ) of the epithelial bcsfb established by choroid plexus epithelial cells .
the microvessels within the highly vascularized choroid plexus are fenestrated capillaries that allow the free diffusion of water - soluble molecules across the vascular wall . nevertheless , these vascular endothelial cells form ajs and tjs resembling those of peripheral vascular beds and are thus not drawn in detail here .
ajs of the bcsfb are formed by the homophilic interaction of cis homodimers of the transmembrane protein e - cadherin . via its cytoplasmic tail
, e - cadherin binds the armadillo family members p120- and -catenin , which , in turn bind -catenin , linking e - cadherin to the epithelial actin cytoskeleton .
oriented apically relative to the ajs , the tj strands of the bcsfb run in parallel around the entire circumference of the choroid plexus epithelial cells , with characteristic gaps that have been suggested to be important in the context of the transport functions of the choroid plexus .
the transmembrane proteins that have been characterized as localized to the bcsfb tjs include occludin , claudin-1 , claudin-2 , claudin-3 , and claudin-11 as well as jam - a and jam - c . via their carboxy terminus , which harbors a pdz domain binding motif , these proteins bind the scaffolding proteins zo-1 , zo-2 , and probably zo-3 , which link to the actin cytoskeleton .
tjs regulate the diffusion of solutes and ions through the paracellular route , which is referred to as their  gate  function .
tjs also establish a  fence  function by limiting the free movements of lipids and proteins from the apical and basolateral cell surfaces , thus contributing to cell polarity ( dejana et al .
in addition to the tjs between two adjacent cells , a special molecularly distinct tj has been described at tricellular contacts , where the corners of three endothelial or epithelial cells meet .
interestingly , these tricellular tjs exist in bbb endothelial cells and bcsfb epithelial cells ( iwamoto et al . , 2014 ) , suggesting that tricellular tjs may be required for full barrier function of both types of brain barriers . before tj formation , ajs initiate cell - to - cell contacts and promote their maturation , maintenance , and plasticity , and regulate tensile forces . yet ,
far less is known about the underlying role of ajs in regulating dynamics of cell  contacts and the establishment of cell polarity at the brain barriers . in epithelial cells , including the choroid plexus epithelium , tjs are the most apical component of the junctional complex and thus clearly distinguishable from ajs . in endothelial cells ,
however , localization of tjs and ajs is more variable , and both junctional complexes appear intermingled , especially in brain endothelial cell - to - cell junctions ( schulze and firth , 1993 ; vorbrodt and dobrogowska , 2003 ; dejana et al . , 2009 ) .
given that endothelial ajs in peripheral vascular beds play an important role in the control of vascular permeability ( giannotta et al .
, 2013 ) , the contribution of these junctions to the integrity of bbb junctions during development , normal physiology , and disease should be elucidated .
this is underscored by recent evidence demonstrating that the tj protein zonula occludens 1 ( zo-1 ) is also a central regulator of endothelial ajs ( tornavaca et al . , 2015 ) .
at first glance the basic molecular makeup of bbb and bcsfb ajs and tjs resembles that of general endothelial and epithelial barriers ( fig . 1 and table 1 ) , and most work on the function of junctional molecules in regulating brain barriers has been extrapolated from studies on their non - cns counterparts . yet , their high complexity and continuity distinguishes brain barrier tjs from peripheral tjs , and there are also important differences in their molecular makeup .
in addition to the unique combinations of claudins observed in bbb and bcsfb tjs ( fig . 1 and table 1 ) , high expression levels of the integral tj proteins occludin and claudin-5 and low expression levels of the integral aj protein ve - cadherin characterize bbb endothelial cells ( table 1 ) .
this influences spatial arrangement of the cytoplasmic junctional scaffolding proteins , and thus affects characteristics of the anchorage of brain barrier junctional complexes to the cytoskeleton and , ultimately , the downstream signaling events regulating junctional dynamics ( table 1 ; goddard and iruela - arispe , 2013 ; citi et al . , 2014 ) .
such events include junctional protein phosphorylation , the continuous recycling of junctional molecules , and regulation of cytoskeletal dynamics by rho family gtpases ( summarized in citi et al .   and goddard and iruela - arispe ) .
unfortunately , few of these processes have been analyzed specifically in brain endothelial or choroid plexus epithelial cells .
in particular , junction dynamics in brain endothelial cells could be very different , as pinocytotic activity is very low in brain endothelial cells .
if endocytic activity was also low , this would slow down the recycling of junctional components such as cadherins . furthermore , despite the generation of elegant mouse mutants to interrogate the specific role of individual junctional components in regulating aj or tj dynamics , careful analysis of brain barriers has yet to be performed in these models ( schulte et al .
expression and known function of junctional molecules at brain barriers in mice , brain angiogenesis starts at 9.5 d postcoitum , when vascular sprouts invade from the perineural vascular plexus into the developing neuroectoderm , leading to the formation of an undifferentiated vascular network .
the molecular mechanisms driving brain angiogenesis are fairly well understood and have been summarized elsewhere ( engelhardt , 2003 ; engelhardt and liebner , 2014 ) .
how the barrier characteristics of cns endothelial cells , including their unique junctional complexes , mature during brain angiogenesis directed by factors from the developing neuroectoderm is less well understood .
the aj component ve - cadherin is one of the first endothelial cell  specific molecules expressed and required for endothelial survival , blood vessel assembly , and stabilization ( carmeliet et al . , 1999 ;
interestingly , expression of ve - cadherin during brain angiogenesis has been observed to be relatively low ( breier et al . , 1996 ) , and
instead cadherin-10 was abundantly present in ajs of cns endothelial cells ( williams et al . , 2005 ) .
in addition to ve - cadherin , n - cadherin is expressed in cns endothelial cells during brain angiogenesis ( gerhardt et al . , 2000 ) .
it accumulates at contact zones between endothelial cells and the surrounding pericytes , where it regulates expression of ve - cadherin at the cell membrane ( luo and radice , 2005 ) and thus contributes to aj maturation ( gerhardt et al . , 2000 ;
stable ajs are considered to be required for the formation of tjs . a first hint to how endothelial ajs might induce tj formation at the molecular level
is provided by the observation that ve - cadherin , through akt activation and by inhibition of -catenin translocation to the nucleus , causes phosphorylation of the transcription factor forkhead box factor 1 ( foxo1 ) , allowing it to activate expression of the tj protein claudin-5 ( taddei et al . , 2008 ) . this molecular link between ajs and tjs
this is supported by studies in zebrafish , where expression of claudin-5 and zo-1 has been described in cns microvessels starting at day 3 postfertilization , which was concomitant with the maturation of the bbb as determined by restricted permeability to small and large size tracers ( jeong et al . , 2008 ) .
in contrast to our limited knowledge about the molecular mechanisms leading to aj maturation at the bbb , the mechanism of induction of expression of tj proteins such as claudin-3 , which are unique to cns microvascular tjs , is much better understood .
wnt ligands produced by immature neuroectodermal cells bind to frizzled receptors ( fzd ) on cns vascular endothelial cells , inducing canonical wnt signaling .
this leads to translocation of -catenin into the nucleus of brain endothelial cells resulting in the induction of bbb - specific gene transcription , including the tj protein claudin-3 ( liebner et al . , 2008 ; stenman et al . , 2008 ; daneman et al . ,
in addition , the death receptors troy ( tnfrsf19 ) and dr6 ( tnfrs21 ) , which are downstream targets of the wnt/-catenin signaling pathway and thus involved in brain angiogenesis in mice and zebrafish ( tam et al . , 2012 ) , have been linked to regulating expression of zo-1 in brain endothelial cells .
other factors such as the alternative frizzled-4 ligand norrin or gpr 124 , an orphan member of the g protein  coupled receptor family , which are coactivators of the canonical wnt/-catenin signaling pathway in nonoverlapping cns regions , also contribute to the induction of bbb - specific junctional molecules such as claudin-5 ( zhou and nathans , 2014 ; zhou et al . , 2014 ) .
the sonic hedgehog ( shh ) pathway has also been implicated in brain angiogenesis and bbb maturation ( alvarez et al . ,
this study demonstrated that , during brain angiogenesis , shh is secreted by immature astrocytes , and that lack of the shh signal transducer smoothened ( smo ) in cns endothelial cells leads to reduced expression of the tj proteins occludin , claudin-3 , and claudin-5 , but also of zo-1 and p120-catenin , which was accompanied by increased leakage of plasma tracers in the developing bbb . by regulating expression of p120-catenin
, the shh pathway might influence maturation of both cns endothelial ajs and tjs . at the same time , this study provided in vitro evidence that shh increases the expression of the tj proteins occludin , claudin-3 , claudin-5 , and jam - a in human brain endothelial cells ( alvarez et al . ,
another single study that awaits confirmation showed that astrocyte - derived ssecks ( src - suppressed c - kinase substrate ) contributes to cns endothelial junctional maturation by regulating the release of astrocyte - derived ang-1 , which , by binding to its receptor tie-2 on the cns endothelial cells , increases expression of the tj proteins occludin , claudin-1 , zo-1 , and zo-2 in human endothelial cells ( lee et al . , 2003 ) .
a further step in the maturation of the bbb is the interaction of endothelial cells from nascent vessels with pericytes .
pericytes are recruited to the sprouting cns vessels during brain angiogenesis and contribute to bbb maturity including the regulation of astrocyte polarity ( armulik et al .
in addition to the homophilic interaction between endothelial and pericyte n - cadherins , platelet - derived growth factor - b ( pdgf - b ) secreted by endothelial cells and its receptor pdgfr- on the pericytes regulates pericyte / endothelial interaction ( summarized in siegenthaler et al . , 2013 ) .
in contrast to astrocyte - derived factors , pericytes seem to have no influence on the maturation of bbb ajs and tjs , as pericyte - deficient mice develop a normal bbb tj architecture ( armulik et al . , 2010 ) , while showing increased bbb leakiness due to , surprisingly , increased vesicular trafficking across cns endothelial cells .
this has recently been underlined by the finding that mfsd2a ( major facilitator super family domain containing 2a ) is specifically induced in cns endothelial cells by endothelial / pericyte interactions and suppresses transcytotic activity in cns endothelial cells , thus contributing to bbb maturation ( ben - zvi et al . , 2014 ) .
although there are numerous studies dedicated to the investigation of barrier integrity of the bcsfb during embryonic development , few studies have specifically addressed maturation of cell - to - cell junctions in choroid plexus epithelial cells and whether this correlates with the barrier properties of the bcsfb .
complex tjs have been visualized by freeze - fracture and transmission electron microscopy during very early human and sheep fetal development , at a time when peripheral tracers were still found to diffuse across bcsfb obviously via a transcellular route ( mllgrd and saunders , 1975 ) .
these observations indirectly imply that bcsfb ajs connect choroid plexus epithelial cells early during development before barrier maturation .
more recent studies that focused on investigating expression of tj proteins during embryonic development showed expression of occludin and marveld3 in the choroid plexus of mouse embryos ( diez - roux et al . , 2011 ) , while in rats early and high expression levels of up to 10 different claudins as well as of occludin and zo-1 , zo-2 , and zo-3 were observed and thus suggest the early development of mature tj complexes at the bcsfb ( kratzer et al .
specifically , in addition to claudin-1 , claudin-2 , claudin-3 , and claudin-11 , these studies discovered expression of claudin-9 , claudin-19 , and claudin-22 in choroid plexus epithelial cells .
investigation of the maturation of the bcsfb in zebrafish demonstrated that this barrier becomes functional starting at day 2 postfertilization as shown by reduced leakage of different sized tracers ( henson et al . , 2014 ) .
interestingly , this study also describes immunostaining for claudin-5 , which in rodents has been found to be specifically expressed in endothelial cells , in the choroid plexus epithelium ( henson et al . ,
considering its proposed role in bbb maturation , one of the signaling pathways involved in regulating junctional maturation in the bcsfb might be the shh pathway , which was found to be critical in the continual growth and expansion of the hindbrain choroid plexus throughout development ( huang et al . , 2009 ) . a combination of transcriptome and proteome analyses of the bbb and the bcsfb ( huntley et al
, the development of antibodies to detect the subcellular localization of the individual molecular components of junctions , as well as high - resolution imaging have improved our knowledge of the junctional architecture of bbb endothelial cells and the choroid plexus epithelial cells ( fig . 1 and table 1 ) .
choroid plexus epithelial cells seem to maintain their unique barrier characteristics , including proper junctional complexes , when removed from the cns and put in culture ( gath et al . , 1997 ; strazielle and ghersi - egea , 1999 ; schroten et al . , 2012 ) . however , endothelial cells forming the bbb need pericytes and astrocytes as well as the extracellular matrix surrounding the bbb endothelium to maintain their unique barrier characteristics ( wolburg et al . , 1994 ; liebner et al . , 2000b ) .
adhesive contacts between pericytes and endothelial cells are provided by n - cadherin , which in this case is not restricted to ajs ( gerhardt et al . , 2000 ) .
studies in viable pericyte - deficient mouse mutants have shown that pericytes do not directly affect bbb junctional architecture but rather regulate barrier characteristics of cns endothelial cells by inhibiting transcellular vesicular trafficking and inducing astrocyte polarity ( armulik et al .
however , astrocyte - derived factors seem to maintain junctional integrity of the bbb endothelium . indeed , toxin - induced deletion of gfap astrocytes in certain brain regions in mice and rats was found to induce a reversible focal dysfunction of the bbb ( bush et al . , 1999 ; willis et al . , 2013 ) that is accompanied by the loss of junctional localization of claudin-5 and altered phosphorylation of occludin in brain endothelium ( willis et al . , 2013 ) .
interestingly , although junctional localization of ve - cadherin and -catenin was found unchanged in the latter study , expression of ve - cadherin was up - regulated , underlining the tight connection between regulating bbb ajs and tjs .
the molecular cues provided by astrocytes to maintain junctional integrity of the bbb are not well understood .
in addition to its role in bbb development , recent studies support a role of the canonical wnt/-catenin signaling pathway in bbb maintenance and thus probably in sustaining junctional integrity . using novel transgenic reporter lines to visualize wnt/-catenin signaling in zebrafish allowed for the demonstration of wnt/-catenin signaling activity in cns vessels of adult zebrafish ( moro et al . , 2012 ) .
furthermore , elegant gain- and loss - of - function mouse mutants for norrin / fz4 signaling show a cell - autonomous gain or loss of bbb function accompanied by the presence or absence of claudin-5 expression , respectively , in the cerebellum of these mice , which supports the continuous requirement of frizzled signaling in preserving the mature bbb junctional structure ( wang et al .
finally , components of the basement membranes have recently been shown to contribute to junctional integrity of brain endothelial cells ( fig .
the heparan sulfate proteoglycan agrin contributes to barrier properties of brain endothelial cells by stabilizing junctional localization of molecules forming ajs rather than tjs ( steiner et al . , 2014 ) . using mouse models in which 1-integrins are specifically inactivated in endothelial cells , yamamoto et al .
( 2015 ) showed that the absence of this integrin reduced the interaction between ve - cadherin and p120-catenin , causing an increase in the internalization of ve - cadherin .
thus , 1-integrin  mediated binding of cns endothelial cells to the extracellular matrix is critical for stabilizing junctional localization of ve - cadherin and vascular integrity ( yamamoto et al . , 2015 ; fig .
2 ) . the molecular processes identified to be downstream of endothelial 1-integrin involved myosin light chain phosphorylation by the gtpase rap1 and the kinase mrck in addition to rho / rho - kinase signaling . in light of the previous observations that junctional localization of ve - cadherin regulates expression and junctional localization of claudin-5 ( taddei et al . , 2008 ) , it is interesting to note that endothelial cell matrix interactions via endothelial 1-integrins were also found to increase expression and junctional localization of claudin-5 to bbb tjs ( osada et al . , 2011 ; fig .
2 ) . in addition , laminin 2 , which is part of the astrocyte - derived parenchymal basement membrane and thus critical for astrocyte polarity , influences bbb tj morphology and barrier function , as recently observed in laminin 2deficient mice ( menezes et al . , 2014 ) .
( a ) under physiological conditions , bbb endothelial cells are anchored via 1-integrins to the ecm proteins laminin , collagen , and perlecan in the endothelial basement membrane . 1-integrin
 mediated adhesive contacts contribute by not - yet - defined downstream signaling events to junctional stabilization .
( b ) after ischemic stroke , edema formation and bbb breakdown is associated with loss of vascular 1-integrin , probably by shedding or degradation ( indicated by a question mark ) .
genetic or functional inactivation of 1-integrin at the bbb induces bbb leakiness by reducing the association of p120 and -catenin with ve - cadherin .
this leads to increased internalization of ve - cadherin via clathrin - coated pits and ultimately loss of junctional claudin-5 .
in contrast to the bbb tjs , not much is known about factors regulating junctional integrity at the bcsfb . in general , junctional complexes of epithelial cells including those of the bcsfb are intrinsically more stable , as indicated by the formation of mature ajs and tjs in cultured choroid plexus epithelial cells ( gath et al .
however , besides providing the bcsfb , the choroid plexus has been suggested to regulate access of hormones to the csf . in this context , a study performed in sheep has demonstrated photoperiod - dependent regulation of the expression levels of occludin , zo-1 , zo-2 , afadin , and e - cadherin in the choroid plexus in correlation with the regulation of the passage of hormones into the brains of the sheep ( lagaraine et al . , 2011 ) .
this study therefore provides evidence for a physiological and dynamic regulation of junctional complexes at the bcsfb .
neuroinflammation is generally accompanied by impaired bbb and bcsfb function , which includes alterations in their junctional complexes .
for example , in the vascular dysplasia referred to as cerebral cavernous malformation ( ccm ) , loss - of - function mutations in ccm-1 , ccm-2 , or ccm-3 , which localize generally to ajs , primarily affect brain vessels .
ccms form a tripartite complex that associates with -catenin and afadin at ajs . by regulating the small gtpase rap-1 ( ccm-1 ) and the rho gtpase pathway ( ccm-2 ) , they contribute to junction stabilization and endothelial cell polarity ( dejana and orsenigo , 2013 ) .
mutations in ccm proteins lead to endothelial  mesenchymal transition , which is characterized by loss of ve - cadherin , up - regulated expression of n - cadherin , and the accompanying loss of endothelial cell polarity ( maddaluno et al . , 2013 ) .
thus , even though the ccm genes are mutated in endothelial cells throughout the body , barrier function of brain endothelial cells seems to more critically rely on the adhesive properties of ve - cadherin to stabilize cell polarity ( dejana and orsenigo , 2013 ) .
disruption of bbb and bcsfb function also contributes to neurological disorders such as stroke or multiple sclerosis ( ms ) and its animal model experimental autoimmune encephalomyelitis ( eae ) .
ms is an inflammatory demyelinating disease of the cns , in which immune cells cross the bbb and the bcsfb causing cns inflammation , edema formation , and brain barrier dysfunction .
in fact , early lesion formation in ms is associated with focal bbb dysfunction as visualized by gadolinium - enhanced magnetic resonance imaging ( kermode et al . , 1990 ) , which is used as a diagnostic criterion for this disease .
a series of immunofluorescence studies of postmortem brain samples from ms patients have convincingly demonstrated that an abnormal distribution of the tj proteins occludin , jam - a , and zo-1 but not of the aj protein -catenin correlates with the premortem pattern of perivascular serum protein leakage in active ms lesions ( plumb et al . , 2002 ; kirk et al . , 2003 ;
these data therefore strongly support a contribution of tj disruption to bbb dysfunction in ms . based on immunostainings for zo-1 ,
bbb junction alterations as observed in ms are quite reliably modeled in brain tissues of mice after induction of eae ( bennett et al . , 2010 ) .
using the eae model , a critical function for bbb tjs in maintaining barrier functions of the bbb in neuroinflammation has been further supported by the observation that inducible endothelial cell  specific ectopic expression of claudin-1 ameliorated clinical symptoms of eae in mice by specifically blocking bbb leakiness without affecting immune cell migration into the cns ( pfeiffer et al . , 2011 ) .
immune cell infiltration into the cns during eae might , however , add to bbb tj pathology , as further studies in the eae model showed the selective loss of claudin-3 or claudin-5 immunostaining in brain and spinal cord microvessels surrounded by inflammatory infiltrates ( wolburg et al . , 2003 ; paul et al . , 2013 ) .
loss of immunostaining for an individual tj protein is , however , not predictive for a dysfunctional bbb , as recent studies on eae pathogenesis in claudin-3deficient mice failed to define a role for claudin-3 in maintaining bbb integrity or regulating leukocyte infiltration into the cns during eae ( kooij et al . ,
also , considering the known function of claudin-5 in regulating the diffusion of small molecules ( < 800 d ) across the bbb , specific loss of claudin-5 immunostaining in bbb tjs can hardly be the equivalent of bbb leakiness for large serum proteins such as fibrinogen into the cns parenchyma .
rather , these observations suggest that the loss of additional junctional components is mandatory for an impairment of bbb function as observed in ms and eae .
indeed , a recent study has found severe loss of both the tj protein claudin-5 and the aj protein ve - cadherin in microvessels in preactive and active inflammatory lesions in postmortem brain tissues of ms patients , which supports the notion that both bbb ajs and tjs are impaired in ms and that alterations in the molecular composition of tjs are early events in bbb dysfunction during ms ( alvarez et al . , 2015 ) .
a possible molecular mechanism behind these junctional changes might be the loss of 1-integrin  mediated anchorage of brain endothelial cells to the basement membrane , as observed in stroke ( osada et al . , 2011 ) , leading to the internalization of ve - cadherin and claudin-5 as outlined earlier ( fig .
, loss of claudin-5 and occludin at the bbb during eae was found to correlate with increased expression of caveolin-1 and increased leakage of fitc - dextran across the bbb , which suggests that caveolae - mediated endocytosis of claudin-5 and occludin contributes to bbb tj breakdown in eae ( errede et al . , 2012 ) .
another molecule that has been shown to induce disruption of bbb tjs during neuroinflammation by down - regulating claudin-5 and occludin is vegf , expression of which is significantly up - regulated in astrocytes during eae and ms ( argaw et al . , 2009 , 2012 ) .
in peripheral vascular beds , vegf induces tyrosine phosphorylation of ve - cadherin , leading to disruption of p120-catenin and -catenin binding , subsequent internalization and degradation of ve - cadherin , and thus increased vascular permeability ( summarized in goddard and iruela - arispe , 2013 ) .
in contrast to peripheral vascular beds , the bbb endothelium responds to vegf - a in a highly polarized fashion  e.g .
, only abluminal from the cns , but not luminal vegf - a , induces increased bbb permeability by activation of p38 mapk and loss of junctional localization of claudin-5 ( hudson et al . , 2014 ) .
thus , in neuroinflammation , astrocyte - derived factors seem to be instrumental in regulating bbb junctional integrity .
this is further supported by the observations that bbb dysfunction is associated with loss of astrocyte polarity .
this is visualized by the decrease of the polarized localization of the water channel aquaporin 4 ( aqp4 ) from astrocyte end - feet in eae and other neurological disorders such as stroke and glioblastoma multiforme ( wolburg - buchholz et al . , 2009 ) .
loss of astrocyte polarity suggests loss of polarized secretion of other astrocyte - derived factors such as shh , wnts , and norrin that support appropriate expression and localization of bbb junctional molecules .
indeed , up - regulated expression of shh in hypertrophic astrocytes in active demyelinating ms lesions was accompanied by increased expression of the shh receptor patched-1 and nuclear translocation of the shh pathway transcription factor gli-1 in bbb endothelial cells ( alvarez et al . , 2011 ) .
these observations suggest that neuroinflammation activates the shh pathway at the level of the bbb , thereby possibly promoting bbb repair .
the concept that neuroinflammation might reactivate pathways operative during the maturation of bbb junctional complexes in embryonic development is further supported by the recent observations that retinoic acid ( ra ) contributes to bbb maturation .
radial glial cells in fetal human brain tissue were found to express high levels of the ra - producing enzyme retinaldehyde dehydrogenase 2 ( raldh2 ) and to tightly associate with the developing brain vasculature expressing the ra receptor  during embryogenesis ( mizee et al . , 2013 ) .
in vitro studies on brain endothelial cells confirmed the ability of ra to increase barrier characteristics , including enhancing expression of aj and tj proteins .
interestingly , expression of raldh2 is found to be significantly up - regulated in reactive astrocytes in active and chronic ms lesions ( mizee et al . , 2014 ) .
these observations further support the notion that neuroinflammation induces increased expression of shh and enhanced release of ra from reactive astrocytes , with the aim of providing a protective response allowing restoration of bbb junctional integrity . finally , the proinflammatory cytokine interleukin-1 ( il-1 ) is up - regulated in the cns during neuroinflammation and critically contributes to brain barrier dysfunction .
it was shown to mediate transcriptional repression of claudin-5 by inducing nuclear translocation of -catenin and foxo1 in brain endothelial cells ( beard et al . , 2014 ) .
these observations suggest that neuroinflammation reverts some processes described for junctional maturation , e.g. , induction of claudin-5 expression ( taddei et al . , 2008 ) during development .
however , as il-1induced nuclear translocation of -catenin was associated with a decrease in claudin-5 and occludin , but wnt3a - mediated nuclear translocation of -catenin rather increased expression of claudin-1 in an in vitro bbb model , -catenin  mediated transcriptional activity in brain endothelial cells seems to be dependent on the respective stimulus ( beard et al . , 2014 ) .
neuroinflammation induces expression of additional inflammatory mediators such as the chemokine ccl2 , especially in astrocytes . at least in vitro
, ccl2 has been shown to induce src - dependent tyrosine phosphorylation of ve - cadherin and -catenin , causing their transient dissociation from ajs ( roberts et al . , 2012 ) .
concurrently , -catenin is recruited to pecam-1 , thereby remaining sequestered at the cell membrane . upon ccl2-dependent tyrosine phosphorylation of pecam-1
, shp-2 is engaged in inducing the release of -catenin and its reassociation with the aj .
this study assigns an important role for pecam-1 in transient sequestration of -catenin : allowing for rapid reorganization of aj complexes in brain endothelium .
in fact , the observation that pecam-1 c57bl/6 mice develop aggravated eae that is accompanied by increased and prolonged bbb permeability ( graesser et al . , 2002 ) highlights this function of endothelial pecam-1 in restoring bbb ajs and further underlines the fundamental role of mature ajs for properly formed junctional complexes at the bbb .
less still is known about possible changes at the level of bcsfb junctions during neuroinflammation .
although several studies have shown dramatic changes of the choroid plexus during ms and eae ( engelhardt et al . , 2001 ; murugesan et al . ,
2012 ) , only a few specifically addressed alterations in the junctional architecture of the choroid plexus epithelial cells ( wolburg et al . , 2001 ; kooij et al . , 2014 ) and reported interrupted immunoreactivities for occludin , claudin-1 , claudin-2 , and claudin-11 as well as for zo-1 and complete loss of claudin-3 immunostaining at the level of the bcsfb during eae and ms . with the presently limited knowledge of the specific function of each of these junctional molecules , the implications of these findings for bcsfb function during neuroinflammation are of course limited . despite great progress in our understanding of the molecular composition of brain barrier junctions , research in the field has mostly focused on studying brain barrier tjs .
there is now strong experimental evidence for crosstalk between ajs and tjs influencing their stability and dynamics at a structural and transcriptional level . beyond their structural functions , ajs and tjs
perturbation of these junctional complexes at the level of the brain barriers thus disrupts cns homeostasis and is associated with neurological disorders .
there is evidence that protecting the brain barriers by specifically improving junctional integrity is beneficial in animal models for ms or stroke ( pfeiffer et al . , 2011 ; wacker et al . , 2012 )
considering the recent findings that circulating sphingosine-1-phosphate ( s1p ) stabilizes vascular junctions via downstream signaling through its g - protein  coupled receptor s1p1 , which influences the cortical actin cytoskeleton ( summarized in goddard and iruela - arispe , 2013 ) , it is tempting to speculate that therapeutic efficacy of the s1p1 agonist fingolimod in ms patients also relies on stabilizing the bbb
. therefore , future research needs to go beyond studying the role of individual tj proteins at the brain barriers . a more integrated cell biological view on the dynamic collaboration of ajs and tjs and how they regulate junctional dynamics and downstream signaling events is required to improve our understanding of how these junctional complexes establish cell polarity and maintain barrier properties of the bbb and the bcsfb .
at first glance the basic molecular makeup of bbb and bcsfb ajs and tjs resembles that of general endothelial and epithelial barriers ( fig . 1 and table 1 ) , and most work on the function of junctional molecules in regulating brain barriers has been extrapolated from studies on their non - cns counterparts . yet , their high complexity and continuity distinguishes brain barrier tjs from peripheral tjs , and there are also important differences in their molecular makeup .
in addition to the unique combinations of claudins observed in bbb and bcsfb tjs ( fig . 1 and table 1 ) , high expression levels of the integral tj proteins occludin and claudin-5 and low expression levels of the integral aj protein ve - cadherin characterize bbb endothelial cells ( table 1 ) .
this influences spatial arrangement of the cytoplasmic junctional scaffolding proteins , and thus affects characteristics of the anchorage of brain barrier junctional complexes to the cytoskeleton and , ultimately , the downstream signaling events regulating junctional dynamics ( table 1 ; goddard and iruela - arispe , 2013 ; citi et al . , 2014 ) .
such events include junctional protein phosphorylation , the continuous recycling of junctional molecules , and regulation of cytoskeletal dynamics by rho family gtpases ( summarized in citi et al .   and goddard and iruela - arispe ) .
unfortunately , few of these processes have been analyzed specifically in brain endothelial or choroid plexus epithelial cells .
in particular , junction dynamics in brain endothelial cells could be very different , as pinocytotic activity is very low in brain endothelial cells .
if endocytic activity was also low , this would slow down the recycling of junctional components such as cadherins . furthermore , despite the generation of elegant mouse mutants to interrogate the specific role of individual junctional components in regulating aj or tj dynamics , careful analysis of brain barriers has yet to be performed in these models ( schulte et al . , 2011 ;
in mice , brain angiogenesis starts at 9.5 d postcoitum , when vascular sprouts invade from the perineural vascular plexus into the developing neuroectoderm , leading to the formation of an undifferentiated vascular network .
the molecular mechanisms driving brain angiogenesis are fairly well understood and have been summarized elsewhere ( engelhardt , 2003 ; engelhardt and liebner , 2014 ) .
how the barrier characteristics of cns endothelial cells , including their unique junctional complexes , mature during brain angiogenesis directed by factors from the developing neuroectoderm is less well understood .
the aj component ve - cadherin is one of the first endothelial cell  specific molecules expressed and required for endothelial survival , blood vessel assembly , and stabilization ( carmeliet et al . , 1999 ; crosby et al . , 2005 ) .
interestingly , expression of ve - cadherin during brain angiogenesis has been observed to be relatively low ( breier et al . , 1996 ) , and
instead cadherin-10 was abundantly present in ajs of cns endothelial cells ( williams et al . , 2005 ) .
in addition to ve - cadherin , n - cadherin is expressed in cns endothelial cells during brain angiogenesis ( gerhardt et al . , 2000 ) .
it accumulates at contact zones between endothelial cells and the surrounding pericytes , where it regulates expression of ve - cadherin at the cell membrane ( luo and radice , 2005 ) and thus contributes to aj maturation ( gerhardt et al . , 2000
stable ajs are considered to be required for the formation of tjs . a first hint to how endothelial ajs might induce tj formation at the molecular level
is provided by the observation that ve - cadherin , through akt activation and by inhibition of -catenin translocation to the nucleus , causes phosphorylation of the transcription factor forkhead box factor 1 ( foxo1 ) , allowing it to activate expression of the tj protein claudin-5 ( taddei et al . , 2008 ) . this molecular link between ajs and tjs
this is supported by studies in zebrafish , where expression of claudin-5 and zo-1 has been described in cns microvessels starting at day 3 postfertilization , which was concomitant with the maturation of the bbb as determined by restricted permeability to small and large size tracers ( jeong et al . , 2008 ) .
in contrast to our limited knowledge about the molecular mechanisms leading to aj maturation at the bbb , the mechanism of induction of expression of tj proteins such as claudin-3 , which are unique to cns microvascular tjs , is much better understood .
wnt ligands produced by immature neuroectodermal cells bind to frizzled receptors ( fzd ) on cns vascular endothelial cells , inducing canonical wnt signaling .
this leads to translocation of -catenin into the nucleus of brain endothelial cells resulting in the induction of bbb - specific gene transcription , including the tj protein claudin-3 ( liebner et al . , 2008 ;
in addition , the death receptors troy ( tnfrsf19 ) and dr6 ( tnfrs21 ) , which are downstream targets of the wnt/-catenin signaling pathway and thus involved in brain angiogenesis in mice and zebrafish ( tam et al . , 2012 ) , have been linked to regulating expression of zo-1 in brain endothelial cells .
other factors such as the alternative frizzled-4 ligand norrin or gpr 124 , an orphan member of the g protein  coupled receptor family , which are coactivators of the canonical wnt/-catenin signaling pathway in nonoverlapping cns regions , also contribute to the induction of bbb - specific junctional molecules such as claudin-5 ( zhou and nathans , 2014 ; zhou et al . , 2014 ) .
the sonic hedgehog ( shh ) pathway has also been implicated in brain angiogenesis and bbb maturation ( alvarez et al . , 2011 ) .
this study demonstrated that , during brain angiogenesis , shh is secreted by immature astrocytes , and that lack of the shh signal transducer smoothened ( smo ) in cns endothelial cells leads to reduced expression of the tj proteins occludin , claudin-3 , and claudin-5 , but also of zo-1 and p120-catenin , which was accompanied by increased leakage of plasma tracers in the developing bbb . by regulating expression of p120-catenin ,
the shh pathway might influence maturation of both cns endothelial ajs and tjs . at the same time , this study provided in vitro evidence that shh increases the expression of the tj proteins occludin , claudin-3 , claudin-5 , and jam - a in human brain endothelial cells ( alvarez et al .
another single study that awaits confirmation showed that astrocyte - derived ssecks ( src - suppressed c - kinase substrate ) contributes to cns endothelial junctional maturation by regulating the release of astrocyte - derived ang-1 , which , by binding to its receptor tie-2 on the cns endothelial cells , increases expression of the tj proteins occludin , claudin-1 , zo-1 , and zo-2 in human endothelial cells ( lee et al . , 2003 ) .
a further step in the maturation of the bbb is the interaction of endothelial cells from nascent vessels with pericytes .
pericytes are recruited to the sprouting cns vessels during brain angiogenesis and contribute to bbb maturity including the regulation of astrocyte polarity ( armulik et al . , 2010 ;
in addition to the homophilic interaction between endothelial and pericyte n - cadherins , platelet - derived growth factor - b ( pdgf - b ) secreted by endothelial cells and its receptor pdgfr- on the pericytes regulates pericyte / endothelial interaction ( summarized in siegenthaler et al . , 2013 ) .
in contrast to astrocyte - derived factors , pericytes seem to have no influence on the maturation of bbb ajs and tjs , as pericyte - deficient mice develop a normal bbb tj architecture ( armulik et al . , 2010 ) , while showing increased bbb leakiness due to , surprisingly , increased vesicular trafficking across cns endothelial cells .
this has recently been underlined by the finding that mfsd2a ( major facilitator super family domain containing 2a ) is specifically induced in cns endothelial cells by endothelial / pericyte interactions and suppresses transcytotic activity in cns endothelial cells , thus contributing to bbb maturation ( ben - zvi et al . , 2014 ) .
although there are numerous studies dedicated to the investigation of barrier integrity of the bcsfb during embryonic development , few studies have specifically addressed maturation of cell - to - cell junctions in choroid plexus epithelial cells and whether this correlates with the barrier properties of the bcsfb .
complex tjs have been visualized by freeze - fracture and transmission electron microscopy during very early human and sheep fetal development , at a time when peripheral tracers were still found to diffuse across bcsfb obviously via a transcellular route ( mllgrd and saunders , 1975 ) .
these observations indirectly imply that bcsfb ajs connect choroid plexus epithelial cells early during development before barrier maturation .
more recent studies that focused on investigating expression of tj proteins during embryonic development showed expression of occludin and marveld3 in the choroid plexus of mouse embryos ( diez - roux et al . , 2011 ) , while in rats early and high expression levels of up to 10 different claudins as well as of occludin and zo-1 , zo-2 , and zo-3 were observed and thus suggest the early development of mature tj complexes at the bcsfb ( kratzer et al .
, 2012 ; liddelow et al . , 2013 ) . specifically , in addition to claudin-1 , claudin-2 , claudin-3 , and claudin-11 , these studies discovered expression of claudin-9 , claudin-19 , and claudin-22 in choroid plexus epithelial cells .
investigation of the maturation of the bcsfb in zebrafish demonstrated that this barrier becomes functional starting at day 2 postfertilization as shown by reduced leakage of different sized tracers ( henson et al . , 2014 ) .
interestingly , this study also describes immunostaining for claudin-5 , which in rodents has been found to be specifically expressed in endothelial cells , in the choroid plexus epithelium ( henson et al . , 2014 ) .
considering its proposed role in bbb maturation , one of the signaling pathways involved in regulating junctional maturation in the bcsfb might be the shh pathway , which was found to be critical in the continual growth and expansion of the hindbrain choroid plexus throughout development ( huang et al .
in mice , brain angiogenesis starts at 9.5 d postcoitum , when vascular sprouts invade from the perineural vascular plexus into the developing neuroectoderm , leading to the formation of an undifferentiated vascular network .
the molecular mechanisms driving brain angiogenesis are fairly well understood and have been summarized elsewhere ( engelhardt , 2003 ; engelhardt and liebner , 2014 ) .
how the barrier characteristics of cns endothelial cells , including their unique junctional complexes , mature during brain angiogenesis directed by factors from the developing neuroectoderm is less well understood .
the aj component ve - cadherin is one of the first endothelial cell  specific molecules expressed and required for endothelial survival , blood vessel assembly , and stabilization ( carmeliet et al . , 1999 ; crosby et al . , 2005 ) .
interestingly , expression of ve - cadherin during brain angiogenesis has been observed to be relatively low ( breier et al . , 1996 ) , and
instead cadherin-10 was abundantly present in ajs of cns endothelial cells ( williams et al . , 2005 ) .
in addition to ve - cadherin , n - cadherin is expressed in cns endothelial cells during brain angiogenesis ( gerhardt et al . , 2000 ) .
it accumulates at contact zones between endothelial cells and the surrounding pericytes , where it regulates expression of ve - cadherin at the cell membrane ( luo and radice , 2005 ) and thus contributes to aj maturation ( gerhardt et al . , 2000
stable ajs are considered to be required for the formation of tjs . a first hint to how endothelial ajs might induce tj formation at the molecular level
is provided by the observation that ve - cadherin , through akt activation and by inhibition of -catenin translocation to the nucleus , causes phosphorylation of the transcription factor forkhead box factor 1 ( foxo1 ) , allowing it to activate expression of the tj protein claudin-5 ( taddei et al . , 2008 ) . this molecular link between ajs and tjs
this is supported by studies in zebrafish , where expression of claudin-5 and zo-1 has been described in cns microvessels starting at day 3 postfertilization , which was concomitant with the maturation of the bbb as determined by restricted permeability to small and large size tracers ( jeong et al . , 2008 ) .
in contrast to our limited knowledge about the molecular mechanisms leading to aj maturation at the bbb , the mechanism of induction of expression of tj proteins such as claudin-3 , which are unique to cns microvascular tjs , is much better understood .
wnt ligands produced by immature neuroectodermal cells bind to frizzled receptors ( fzd ) on cns vascular endothelial cells , inducing canonical wnt signaling .
this leads to translocation of -catenin into the nucleus of brain endothelial cells resulting in the induction of bbb - specific gene transcription , including the tj protein claudin-3 ( liebner et al . , 2008 ;
in addition , the death receptors troy ( tnfrsf19 ) and dr6 ( tnfrs21 ) , which are downstream targets of the wnt/-catenin signaling pathway and thus involved in brain angiogenesis in mice and zebrafish ( tam et al . , 2012 ) , have been linked to regulating expression of zo-1 in brain endothelial cells .
other factors such as the alternative frizzled-4 ligand norrin or gpr 124 , an orphan member of the g protein  coupled receptor family , which are coactivators of the canonical wnt/-catenin signaling pathway in nonoverlapping cns regions , also contribute to the induction of bbb - specific junctional molecules such as claudin-5 ( zhou and nathans , 2014 ; zhou et al . , 2014 ) .
the sonic hedgehog ( shh ) pathway has also been implicated in brain angiogenesis and bbb maturation ( alvarez et al . , 2011 ) .
this study demonstrated that , during brain angiogenesis , shh is secreted by immature astrocytes , and that lack of the shh signal transducer smoothened ( smo ) in cns endothelial cells leads to reduced expression of the tj proteins occludin , claudin-3 , and claudin-5 , but also of zo-1 and p120-catenin , which was accompanied by increased leakage of plasma tracers in the developing bbb . by regulating expression of p120-catenin ,
the shh pathway might influence maturation of both cns endothelial ajs and tjs . at the same time , this study provided in vitro evidence that shh increases the expression of the tj proteins occludin , claudin-3 , claudin-5 , and jam - a in human brain endothelial cells ( alvarez et al .
another single study that awaits confirmation showed that astrocyte - derived ssecks ( src - suppressed c - kinase substrate ) contributes to cns endothelial junctional maturation by regulating the release of astrocyte - derived ang-1 , which , by binding to its receptor tie-2 on the cns endothelial cells , increases expression of the tj proteins occludin , claudin-1 , zo-1 , and zo-2 in human endothelial cells ( lee et al . , 2003 ) .
a further step in the maturation of the bbb is the interaction of endothelial cells from nascent vessels with pericytes .
pericytes are recruited to the sprouting cns vessels during brain angiogenesis and contribute to bbb maturity including the regulation of astrocyte polarity ( armulik et al . , 2010 ;
in addition to the homophilic interaction between endothelial and pericyte n - cadherins , platelet - derived growth factor - b ( pdgf - b ) secreted by endothelial cells and its receptor pdgfr- on the pericytes regulates pericyte / endothelial interaction ( summarized in siegenthaler et al . , 2013 ) .
in contrast to astrocyte - derived factors , pericytes seem to have no influence on the maturation of bbb ajs and tjs , as pericyte - deficient mice develop a normal bbb tj architecture ( armulik et al . , 2010 ) , while showing increased bbb leakiness due to , surprisingly , increased vesicular trafficking across cns endothelial cells .
this has recently been underlined by the finding that mfsd2a ( major facilitator super family domain containing 2a ) is specifically induced in cns endothelial cells by endothelial / pericyte interactions and suppresses transcytotic activity in cns endothelial cells , thus contributing to bbb maturation ( ben - zvi et al . , 2014 ) .
although there are numerous studies dedicated to the investigation of barrier integrity of the bcsfb during embryonic development , few studies have specifically addressed maturation of cell - to - cell junctions in choroid plexus epithelial cells and whether this correlates with the barrier properties of the bcsfb .
complex tjs have been visualized by freeze - fracture and transmission electron microscopy during very early human and sheep fetal development , at a time when peripheral tracers were still found to diffuse across bcsfb obviously via a transcellular route ( mllgrd and saunders , 1975 ) .
these observations indirectly imply that bcsfb ajs connect choroid plexus epithelial cells early during development before barrier maturation .
more recent studies that focused on investigating expression of tj proteins during embryonic development showed expression of occludin and marveld3 in the choroid plexus of mouse embryos ( diez - roux et al . , 2011 ) , while in rats early and high expression levels of up to 10 different claudins as well as of occludin and zo-1 , zo-2 , and zo-3 were observed and thus suggest the early development of mature tj complexes at the bcsfb ( kratzer et al .
specifically , in addition to claudin-1 , claudin-2 , claudin-3 , and claudin-11 , these studies discovered expression of claudin-9 , claudin-19 , and claudin-22 in choroid plexus epithelial cells .
investigation of the maturation of the bcsfb in zebrafish demonstrated that this barrier becomes functional starting at day 2 postfertilization as shown by reduced leakage of different sized tracers ( henson et al . , 2014 ) .
interestingly , this study also describes immunostaining for claudin-5 , which in rodents has been found to be specifically expressed in endothelial cells , in the choroid plexus epithelium ( henson et al . , 2014 ) .
considering its proposed role in bbb maturation , one of the signaling pathways involved in regulating junctional maturation in the bcsfb might be the shh pathway , which was found to be critical in the continual growth and expansion of the hindbrain choroid plexus throughout development ( huang et al . , 2009 ) .
a combination of transcriptome and proteome analyses of the bbb and the bcsfb ( huntley et al . , 2014 ) ,
the development of antibodies to detect the subcellular localization of the individual molecular components of junctions , as well as high - resolution imaging have improved our knowledge of the junctional architecture of bbb endothelial cells and the choroid plexus epithelial cells ( fig . 1 and table 1 ) .
choroid plexus epithelial cells seem to maintain their unique barrier characteristics , including proper junctional complexes , when removed from the cns and put in culture ( gath et al . , 1997 ; strazielle and ghersi - egea , 1999 ; schroten et al . , 2012 ) .
however , endothelial cells forming the bbb need pericytes and astrocytes as well as the extracellular matrix surrounding the bbb endothelium to maintain their unique barrier characteristics ( wolburg et al . , 1994 ;
adhesive contacts between pericytes and endothelial cells are provided by n - cadherin , which in this case is not restricted to ajs ( gerhardt et al . , 2000 ) .
studies in viable pericyte - deficient mouse mutants have shown that pericytes do not directly affect bbb junctional architecture but rather regulate barrier characteristics of cns endothelial cells by inhibiting transcellular vesicular trafficking and inducing astrocyte polarity ( armulik et al . , 2010 ; daneman et al .
however , astrocyte - derived factors seem to maintain junctional integrity of the bbb endothelium . indeed , toxin - induced deletion of gfap astrocytes in certain brain regions in mice and rats was found to induce a reversible focal dysfunction of the bbb ( bush et al . , 1999 ; willis et al . , 2013 ) that is accompanied by the loss of junctional localization of claudin-5 and altered phosphorylation of occludin in brain endothelium ( willis et al . , 2013 ) .
interestingly , although junctional localization of ve - cadherin and -catenin was found unchanged in the latter study , expression of ve - cadherin was up - regulated , underlining the tight connection between regulating bbb ajs and tjs .
the molecular cues provided by astrocytes to maintain junctional integrity of the bbb are not well understood .
in addition to its role in bbb development , recent studies support a role of the canonical wnt/-catenin signaling pathway in bbb maintenance and thus probably in sustaining junctional integrity . using novel transgenic reporter lines to visualize wnt/-catenin signaling in zebrafish allowed for the demonstration of wnt/-catenin signaling activity in cns vessels of adult zebrafish ( moro et al . , 2012 ) .
furthermore , elegant gain- and loss - of - function mouse mutants for norrin / fz4 signaling show a cell - autonomous gain or loss of bbb function accompanied by the presence or absence of claudin-5 expression , respectively , in the cerebellum of these mice , which supports the continuous requirement of frizzled signaling in preserving the mature bbb junctional structure ( wang et al .
, 2012 ; zhou et al . , 2014 ) . finally , components of the basement membranes have recently been shown to contribute to junctional integrity of brain endothelial cells ( fig .
the heparan sulfate proteoglycan agrin contributes to barrier properties of brain endothelial cells by stabilizing junctional localization of molecules forming ajs rather than tjs ( steiner et al . , 2014 ) .
using mouse models in which 1-integrins are specifically inactivated in endothelial cells , yamamoto et al .
( 2015 ) showed that the absence of this integrin reduced the interaction between ve - cadherin and p120-catenin , causing an increase in the internalization of ve - cadherin .
thus , 1-integrin  mediated binding of cns endothelial cells to the extracellular matrix is critical for stabilizing junctional localization of ve - cadherin and vascular integrity ( yamamoto et al . , 2015 ; fig .
2 ) . the molecular processes identified to be downstream of endothelial 1-integrin involved myosin light chain phosphorylation by the gtpase rap1 and the kinase mrck in addition to rho / rho - kinase signaling . in light of the previous observations that junctional localization of ve - cadherin regulates expression and junctional localization of claudin-5 ( taddei et al . , 2008 ) , it is interesting to note that endothelial cell matrix interactions via endothelial 1-integrins were also found to increase expression and junctional localization of claudin-5 to bbb tjs ( osada et al . , 2011 ; fig .
2 ) . in addition , laminin 2 , which is part of the astrocyte - derived parenchymal basement membrane and thus critical for astrocyte polarity , influences bbb tj morphology and barrier function , as recently observed in laminin 2deficient mice ( menezes et al . , 2014 ) .
( a ) under physiological conditions , bbb endothelial cells are anchored via 1-integrins to the ecm proteins laminin , collagen , and perlecan in the endothelial basement membrane . 1-integrin
 mediated adhesive contacts contribute by not - yet - defined downstream signaling events to junctional stabilization .
( b ) after ischemic stroke , edema formation and bbb breakdown is associated with loss of vascular 1-integrin , probably by shedding or degradation ( indicated by a question mark ) .
genetic or functional inactivation of 1-integrin at the bbb induces bbb leakiness by reducing the association of p120 and -catenin with ve - cadherin .
this leads to increased internalization of ve - cadherin via clathrin - coated pits and ultimately loss of junctional claudin-5 .
in contrast to the bbb tjs , not much is known about factors regulating junctional integrity at the bcsfb . in general , junctional complexes of epithelial cells including those of the bcsfb are intrinsically more stable , as indicated by the formation of mature ajs and tjs in cultured choroid plexus epithelial cells ( gath et al . , 1997 ; strazielle and ghersi - egea , 1999 ) .
however , besides providing the bcsfb , the choroid plexus has been suggested to regulate access of hormones to the csf . in this context , a study performed in sheep has demonstrated photoperiod - dependent regulation of the expression levels of occludin , zo-1 , zo-2 , afadin , and e - cadherin in the choroid plexus in correlation with the regulation of the passage of hormones into the brains of the sheep ( lagaraine et al . , 2011 ) .
this study therefore provides evidence for a physiological and dynamic regulation of junctional complexes at the bcsfb .
neuroinflammation is generally accompanied by impaired bbb and bcsfb function , which includes alterations in their junctional complexes .
for example , in the vascular dysplasia referred to as cerebral cavernous malformation ( ccm ) , loss - of - function mutations in ccm-1 , ccm-2 , or ccm-3 , which localize generally to ajs , primarily affect brain vessels .
ccms form a tripartite complex that associates with -catenin and afadin at ajs . by regulating the small gtpase rap-1 ( ccm-1 ) and the rho gtpase pathway ( ccm-2 ) , they contribute to junction stabilization and endothelial cell polarity ( dejana and orsenigo , 2013 ) .
mesenchymal transition , which is characterized by loss of ve - cadherin , up - regulated expression of n - cadherin , and the accompanying loss of endothelial cell polarity ( maddaluno et al . , 2013 ) .
thus , even though the ccm genes are mutated in endothelial cells throughout the body , barrier function of brain endothelial cells seems to more critically rely on the adhesive properties of ve - cadherin to stabilize cell polarity ( dejana and orsenigo , 2013 ) .
disruption of bbb and bcsfb function also contributes to neurological disorders such as stroke or multiple sclerosis ( ms ) and its animal model experimental autoimmune encephalomyelitis ( eae ) .
ms is an inflammatory demyelinating disease of the cns , in which immune cells cross the bbb and the bcsfb causing cns inflammation , edema formation , and brain barrier dysfunction .
in fact , early lesion formation in ms is associated with focal bbb dysfunction as visualized by gadolinium - enhanced magnetic resonance imaging ( kermode et al . ,
a series of immunofluorescence studies of postmortem brain samples from ms patients have convincingly demonstrated that an abnormal distribution of the tj proteins occludin , jam - a , and zo-1 but not of the aj protein -catenin correlates with the premortem pattern of perivascular serum protein leakage in active ms lesions ( plumb et al .
these data therefore strongly support a contribution of tj disruption to bbb dysfunction in ms . based on immunostainings for zo-1 , bbb junction alterations as observed in ms
are quite reliably modeled in brain tissues of mice after induction of eae ( bennett et al . , 2010 ) .
using the eae model , a critical function for bbb tjs in maintaining barrier functions of the bbb in neuroinflammation has been further supported by the observation that inducible endothelial cell  specific ectopic expression of claudin-1 ameliorated clinical symptoms of eae in mice by specifically blocking bbb leakiness without affecting immune cell migration into the cns ( pfeiffer et al . , 2011 ) .
immune cell infiltration into the cns during eae might , however , add to bbb tj pathology , as further studies in the eae model showed the selective loss of claudin-3 or claudin-5 immunostaining in brain and spinal cord microvessels surrounded by inflammatory infiltrates ( wolburg et al .
loss of immunostaining for an individual tj protein is , however , not predictive for a dysfunctional bbb , as recent studies on eae pathogenesis in claudin-3deficient mice failed to define a role for claudin-3 in maintaining bbb integrity or regulating leukocyte infiltration into the cns during eae ( kooij et al .
, 2014 ) . also , considering the known function of claudin-5 in regulating the diffusion of small molecules ( < 800 d ) across the bbb , specific loss of claudin-5 immunostaining in bbb tjs can hardly be the equivalent of bbb leakiness for large serum proteins such as fibrinogen into the cns parenchyma .
rather , these observations suggest that the loss of additional junctional components is mandatory for an impairment of bbb function as observed in ms and eae .
indeed , a recent study has found severe loss of both the tj protein claudin-5 and the aj protein ve - cadherin in microvessels in preactive and active inflammatory lesions in postmortem brain tissues of ms patients , which supports the notion that both bbb ajs and tjs are impaired in ms and that alterations in the molecular composition of tjs are early events in bbb dysfunction during ms ( alvarez et al . , 2015 ) .
a possible molecular mechanism behind these junctional changes might be the loss of 1-integrin  mediated anchorage of brain endothelial cells to the basement membrane , as observed in stroke ( osada et al . , 2011 ) , leading to the internalization of ve - cadherin and claudin-5 as outlined earlier ( fig .
, loss of claudin-5 and occludin at the bbb during eae was found to correlate with increased expression of caveolin-1 and increased leakage of fitc - dextran across the bbb , which suggests that caveolae - mediated endocytosis of claudin-5 and occludin contributes to bbb tj breakdown in eae ( errede et al . , 2012 ) .
another molecule that has been shown to induce disruption of bbb tjs during neuroinflammation by down - regulating claudin-5 and occludin is vegf , expression of which is significantly up - regulated in astrocytes during eae and ms ( argaw et al .
, vegf induces tyrosine phosphorylation of ve - cadherin , leading to disruption of p120-catenin and -catenin binding , subsequent internalization and degradation of ve - cadherin , and thus increased vascular permeability ( summarized in goddard and iruela - arispe , 2013 ) .
in contrast to peripheral vascular beds , the bbb endothelium responds to vegf - a in a highly polarized fashion 
, only abluminal from the cns , but not luminal vegf - a , induces increased bbb permeability by activation of p38 mapk and loss of junctional localization of claudin-5 ( hudson et al . , 2014 ) .
thus , in neuroinflammation , astrocyte - derived factors seem to be instrumental in regulating bbb junctional integrity .
this is further supported by the observations that bbb dysfunction is associated with loss of astrocyte polarity .
this is visualized by the decrease of the polarized localization of the water channel aquaporin 4 ( aqp4 ) from astrocyte end - feet in eae and other neurological disorders such as stroke and glioblastoma multiforme ( wolburg - buchholz et al . , 2009 ) .
loss of astrocyte polarity suggests loss of polarized secretion of other astrocyte - derived factors such as shh , wnts , and norrin that support appropriate expression and localization of bbb junctional molecules .
indeed , up - regulated expression of shh in hypertrophic astrocytes in active demyelinating ms lesions was accompanied by increased expression of the shh receptor patched-1 and nuclear translocation of the shh pathway transcription factor gli-1 in bbb endothelial cells ( alvarez et al . , 2011 ) .
these observations suggest that neuroinflammation activates the shh pathway at the level of the bbb , thereby possibly promoting bbb repair .
the concept that neuroinflammation might reactivate pathways operative during the maturation of bbb junctional complexes in embryonic development is further supported by the recent observations that retinoic acid ( ra ) contributes to bbb maturation .
radial glial cells in fetal human brain tissue were found to express high levels of the ra - producing enzyme retinaldehyde dehydrogenase 2 ( raldh2 ) and to tightly associate with the developing brain vasculature expressing the ra receptor  during embryogenesis ( mizee et al .
in vitro studies on brain endothelial cells confirmed the ability of ra to increase barrier characteristics , including enhancing expression of aj and tj proteins .
interestingly , expression of raldh2 is found to be significantly up - regulated in reactive astrocytes in active and chronic ms lesions ( mizee et al . , 2014 ) .
these observations further support the notion that neuroinflammation induces increased expression of shh and enhanced release of ra from reactive astrocytes , with the aim of providing a protective response allowing restoration of bbb junctional integrity . finally , the proinflammatory cytokine interleukin-1 ( il-1 ) is up - regulated in the cns during neuroinflammation and critically contributes to brain barrier dysfunction .
it was shown to mediate transcriptional repression of claudin-5 by inducing nuclear translocation of -catenin and foxo1 in brain endothelial cells ( beard et al . , 2014 ) .
these observations suggest that neuroinflammation reverts some processes described for junctional maturation , e.g. , induction of claudin-5 expression ( taddei et al . ,
however , as il-1induced nuclear translocation of -catenin was associated with a decrease in claudin-5 and occludin , but wnt3a - mediated nuclear translocation of -catenin rather increased expression of claudin-1 in an in vitro bbb model , -catenin  mediated transcriptional activity in brain endothelial cells seems to be dependent on the respective stimulus ( beard et al . , 2014 ) .
neuroinflammation induces expression of additional inflammatory mediators such as the chemokine ccl2 , especially in astrocytes .
at least in vitro , ccl2 has been shown to induce src - dependent tyrosine phosphorylation of ve - cadherin and -catenin , causing their transient dissociation from ajs ( roberts et al . , 2012 ) .
concurrently , -catenin is recruited to pecam-1 , thereby remaining sequestered at the cell membrane . upon ccl2-dependent tyrosine phosphorylation of pecam-1
, shp-2 is engaged in inducing the release of -catenin and its reassociation with the aj .
this study assigns an important role for pecam-1 in transient sequestration of -catenin : allowing for rapid reorganization of aj complexes in brain endothelium .
in fact , the observation that pecam-1 c57bl/6 mice develop aggravated eae that is accompanied by increased and prolonged bbb permeability ( graesser et al .
, 2002 ) highlights this function of endothelial pecam-1 in restoring bbb ajs and further underlines the fundamental role of mature ajs for properly formed junctional complexes at the bbb .
less still is known about possible changes at the level of bcsfb junctions during neuroinflammation .
although several studies have shown dramatic changes of the choroid plexus during ms and eae ( engelhardt et al . , 2001 ; murugesan et al . ,
2012 ) , only a few specifically addressed alterations in the junctional architecture of the choroid plexus epithelial cells ( wolburg et al . , 2001 ; kooij et al . ,
2014 ) and reported interrupted immunoreactivities for occludin , claudin-1 , claudin-2 , and claudin-11 as well as for zo-1 and complete loss of claudin-3 immunostaining at the level of the bcsfb during eae and ms . with the presently limited knowledge of the specific function of each of these junctional molecules ,
despite great progress in our understanding of the molecular composition of brain barrier junctions , research in the field has mostly focused on studying brain barrier tjs .
there is now strong experimental evidence for crosstalk between ajs and tjs influencing their stability and dynamics at a structural and transcriptional level . beyond their structural functions , ajs and tjs
perturbation of these junctional complexes at the level of the brain barriers thus disrupts cns homeostasis and is associated with neurological disorders .
there is evidence that protecting the brain barriers by specifically improving junctional integrity is beneficial in animal models for ms or stroke ( pfeiffer et al . , 2011 ; wacker et al . , 2012 )
considering the recent findings that circulating sphingosine-1-phosphate ( s1p ) stabilizes vascular junctions via downstream signaling through its g - protein  coupled receptor s1p1 , which influences the cortical actin cytoskeleton ( summarized in goddard and iruela - arispe , 2013 ) , it is tempting to speculate that therapeutic efficacy of the s1p1 agonist fingolimod in ms patients also relies on stabilizing the bbb
. therefore , future research needs to go beyond studying the role of individual tj proteins at the brain barriers . a more integrated cell biological view on the dynamic collaboration of ajs and tjs and how they regulate junctional dynamics and downstream signaling events is required to improve our understanding of how these junctional complexes establish cell polarity and maintain barrier properties of the bbb and the bcsfb .
 unique intercellular junctional complexes between the central nervous system ( cns ) microvascular endothelial cells and the choroid plexus epithelial cells form the endothelial blood  brain barrier ( bbb ) and the epithelial blood  cerebrospinal fluid barrier ( bcsfb ) , respectively . 
 these barriers inhibit paracellular diffusion , thereby protecting the cns from fluctuations in the blood . 
 studies of brain barrier integrity during development , normal physiology , and disease have focused on bbb and bcsfb tight junctions but not the corresponding endothelial and epithelial adherens junctions . 
 the crosstalk between adherens junctions and tight junctions in maintaining barrier integrity is an understudied area that may represent a promising target for influencing brain barrier function . 
Introduction
CNS endothelial and epithelial AJs and TJs: Components and dynamics
Maturation of brain barrier junctional complexes during development
Vascular junctions at the developing BBB.
Interepithelial junctions at the developing BCSFB.
Maintenance of junctional complexes at mature brain barriers
Neuroinflammation: junctional complexes at dysfunctional brain barriers
Concluding remarks