the chinese population is aging , which has resulted in an increase in osteoporosis and fractures , particularly vertebral and hip fractures . a large - scale epidemiological survey of the han population showed that in women over the age of 50 yr , the prevalence of osteoporosis was 30.8% , the incidence of vertebral osteoporosis was 27.3% and the prevalence of low bone mass was 64.6% ( 1 ) .
women over the age of 50 , with osteoporotic vertebral fractures , accounted for approximately 3639% of all of osteoporotic fracture cases ( 2 ) .
rewrite : the fracture risk was influenced by both rate of bone loss and peak bone mineral density ( pbmd ) that will be higher in individuals with a low pbmd than normal even if the low rate of bone loss in the following years .
pbmd is a complex quantitative trait that is determined from early childhood and involves the in teraction of multiple genetic and environmental components ( 3 ) .
environmental factors include calcium intake , diet , physical activity and other aspects of life style ( 4 , 5 ) .
the measurement of pbmd is also affected by the site of evaluation . assessing the bone mineral density ( bmd ) of spine , it has a heritability of approximately 40% ( 3 ) .
there were many studies had been performed to identify the relationship between candidate gene polymorphisms and bmd in postmenopausal women with osteoporosis .
proteins such as estrogen receptors , osteoprotegerin , lipoprotein receptor - related protein and sclerostin had been assessed for polymorphisms ( 68 ) .
recently , koller et al . ( 9 ) investigated the effect of 50 single - nucleotide polymorphisms ( snps ) on bmd , an association was observed between bmd and snps that were identified in the sperm ion channel protein ( catsperb ) gene , on chromosome 14 .
the role of catsperb is poorly understood in bone , but its expression in the ovary indicates a potential role in hormonal regulation of bone metabolism ( 9 , 10 ) . the nuclear receptor subfamily 5 member a2 ( nr5a2 ) gene is a member of the nr5a subfamily and
rewrite : nr5a2 gene play a very important role in the process of regulation in the gonadal steroidogenic gene expression .
 it is thougt to play a key role in the regulation of gonadal steroidogenic gene expression 
( 12 ) reported that nr5a2 is expressed at a high level in bone and modulates the expression of some osteoblastic genes .
the rs3762397 snp is a novel loci that affects bmd and is located in the nr5a2 gene , at chromosome site 1q32 ( 11 , 13 ) .
currently , the genetic research on osteoporosis were more concentrated in postmenopausal women ( 14 ) , only a few studies had investigated the relationship between candidate gene polymorphisms , which underlie pbmd variation , and environmental factors .
the objective of this study was to investigate the association between the rs1298989 snp of the catsperb gene and rs3762397 snp of the nr5a2 gene with bmd in female han and uyghur college students .
at the same time the relationships between bmd , gene polymorphisms and life styles also been analyzed .
a total of 370 female xinjiang medical university students , aged between 19.751.21 and 21.461.18 yr , volunteered for the study between the years 2012 and 2013 .
they were recruited from grade four and included five classes of han and four minority classes , of which uyghur subjects accounted for 60%-70% .
samples from 11 individuals could not be amplified and genotyped due to the poor quality of dna .
female college students were excluded if they had any of the following : ( a ) chronic diseases ( e.g. chronic liver disease , chronic renal disease ) , ( b ) an intake of drugs that could influence bone metabolism , ( c ) evidence of other metabolic or inherited bone diseases , ( d ) major gastrointestinal disease , ( e ) any disease , treatment or condition that could be a non - genetic cause of low bone mass .
this study was conducted with approval from the ethics committee of the first affiliated hospital of xinjiang medical university .
all participants answered a questionnaire on age , age at menarche , habitual tea consumption , current drinking habits , current smoking habits , habitual milk consumption , habitual coffee consumption , physical exercise ( regular or not ) and medical history , such as medications and fractures . weight and height were measured in indoor clothes , without shoes , at the time of bone densitometry assessments . the body mass index ( bmi )
following on overnight fasting ( at least 8 hour ) , venous blood samples were obtained for evaluation of bone metabolism .
bone biochemistry was evaluated by serum calcium ( ca ) , phosphorus , alkaline phosphatase , measured by a commercial elisa kit .
the bmd of the lumbar spine ( l2-l4 ) was measured by computed tomography ( ct ) ( 64 light - speed vct , ge ; milwaukee , wi , usa ) .
mineral content was determined in the midspine of the l2-l4 vertebrae , with a 5 mm slice obtained at each level .
a reference phantom that contained calcium hydroxyapatite , at 200 mg / ml , and a water equivalent were used for control measurements .
representative volumes of trabeculae , at the midplane of the three vertebral bodies ( l2-l4 ) , were quantified and averaged .
the results were expressed as mineral equivalents of calcium hydroxyapatite , in mg / cm . based on multiple measurements of 10 subjects , 1.17% was taken as the precious for the lumbar spine .
the participants filled in a standardized food frequency questionnaire ( ffq ) , which included 12 questions that covered nine food items .
all these foods contributed to 75% of the calcium intake and 90% of the vitamin d intake in the most dietary intake studies , performed in denmark .
the questionnaire in ffq included nine predetermined potential frequencies ( ranging from  less than one time per month  to  four to five times per day or more  ) .
genomic dna was extracted from blood using the salting - out method and dna was stored at-20c until use .
the working concentration of dna was 5 - 10ng/l , the concentrations of primers is 0.8m .
the genotypes of the snps were determined using snapshot ( applied biosystems , forster city , ca ) .
this technology uses the polymerase chain reaction ( pcr ) and multiplexed single - base extension , followed by capillary electrophoresis .
primers for the rs1298989 snp of nr5a2 and the rs3762397 snp of catsperb were designed using primer 3 ( http://frodo.wi.m-it.edu/cgi-bin/primer3/primer3_www.cgi ) .
the pcr premix contained 20 l of 10 buffer i ( abi co. , ltd . , foster city , usa ) , 3.0 mm mgcl2 , 0.3 mm dntps , 1 unit of hotstartaq dna polymerase ( qiagen co. , ltd . ,
valencia , spain ) , 1 l of dna and 2 l of multi - pcr primer .
the pcr thermocycling conditions were : 95 c for 2 min ; 11 cycles of 94 c for 20 s , 65 c ( reduced by 0.5 c each cycle ) for 40 s , 72 c for 90 s ; followed by 24 cycles of 94 c for 20 s , 59 c for 30 s , 72 c for 90 s , and a final extension at 72 c for 2 min .
multiplex single - base primer extension reactions were performed using the snapshot multiplex kit ( abi co. , ltd . , foster city , usa ) .
the reactions were then treated with one unit of calf intestinal phosphatase ( new england biolabs , ma , usa ) , at 37 c for 60 min , followed by 15 min at 75 c .
labelled extension products were resolved by capillary electrophoresis , on an dna analyzer ( abi co. , ltd . , foster city , usa ) , and data analysis was performed using genemapper analysis software , version 4.0 ( biosystems co. , ltd .
, usa ) participants were divided into two groups in accordance with the ethnicity given in the baseline survey : ( a ) han nationality ( b ) uyghur nationality .
data for all variables were normally distributed , which allowed parametric tests of significance to be performed .
comparisons of the basic characteristics , bmd and serum biology , between the groups , were calculated through t - tests or t  -tests . to determine whether the allele distributions of the snps satisfied the hardy  weinberg equilibrium ,
a chi - squared test was used to compare the observed and expected allele frequencies .
the association between each snp and physical characteristics ( age , weight and height ) , in addition to dietary calcium intake , energy intake , vitamin d intake and bmd , was assessed by an analysis of variance ( anova ) .
differences in bmd and snp alleles , in relation to ethnicity , were assessed by t - tests .
regression analysis was used to determine the factors that predict bmd in the lumbar spine , evaluate their significance and assess the proportion of variance that these factors can explain .
the regression model analyzed the following variables : ethnic group , rs3762397 geno - type , rs1298989 genotype , age , bmi , age at menarche , total calcium in - take , energy intake , vitamin d intake , regular exercise , current smoking habits , current drinking habits , habitual tea consumption and habitual milk consumption .
all statistical analyses were carried out using spss for windows , version 13.0 ( spss , chicago , il , usa ) .
a total of 370 female xinjiang medical university students , aged between 19.751.21 and 21.461.18 yr , volunteered for the study between the years 2012 and 2013 .
they were recruited from grade four and included five classes of han and four minority classes , of which uyghur subjects accounted for 60%-70% .
samples from 11 individuals could not be amplified and genotyped due to the poor quality of dna .
female college students were excluded if they had any of the following : ( a ) chronic diseases ( e.g. chronic liver disease , chronic renal disease ) , ( b ) an intake of drugs that could influence bone metabolism , ( c ) evidence of other metabolic or inherited bone diseases , ( d ) major gastrointestinal disease , ( e ) any disease , treatment or condition that could be a non - genetic cause of low bone mass .
this study was conducted with approval from the ethics committee of the first affiliated hospital of xinjiang medical university .
all participants answered a questionnaire on age , age at menarche , habitual tea consumption , current drinking habits , current smoking habits , habitual milk consumption , habitual coffee consumption , physical exercise ( regular or not ) and medical history , such as medications and fractures .
weight and height were measured in indoor clothes , without shoes , at the time of bone densitometry assessments . the body mass index ( bmi )
following on overnight fasting ( at least 8 hour ) , venous blood samples were obtained for evaluation of bone metabolism .
bone biochemistry was evaluated by serum calcium ( ca ) , phosphorus , alkaline phosphatase , measured by a commercial elisa kit .
the bmd of the lumbar spine ( l2-l4 ) was measured by computed tomography ( ct ) ( 64 light - speed vct , ge ; milwaukee , wi , usa ) .
mineral content was determined in the midspine of the l2-l4 vertebrae , with a 5 mm slice obtained at each level .
a reference phantom that contained calcium hydroxyapatite , at 200 mg / ml , and a water equivalent were used for control measurements .
representative volumes of trabeculae , at the midplane of the three vertebral bodies ( l2-l4 ) , were quantified and averaged .
the results were expressed as mineral equivalents of calcium hydroxyapatite , in mg / cm . based on multiple measurements of 10 subjects ,
the participants filled in a standardized food frequency questionnaire ( ffq ) , which included 12 questions that covered nine food items .
all these foods contributed to 75% of the calcium intake and 90% of the vitamin d intake in the most dietary intake studies , performed in denmark .
the questionnaire in ffq included nine predetermined potential frequencies ( ranging from  less than one time per month  to  four to five times per day or more  ) .
genomic dna was extracted from blood using the salting - out method and dna was stored at-20c until use .
the working concentration of dna was 5 - 10ng/l , the concentrations of primers is 0.8m .
the genotypes of the snps were determined using snapshot ( applied biosystems , forster city , ca ) .
this technology uses the polymerase chain reaction ( pcr ) and multiplexed single - base extension , followed by capillary electrophoresis .
primers for the rs1298989 snp of nr5a2 and the rs3762397 snp of catsperb were designed using primer 3 ( http://frodo.wi.m-it.edu/cgi-bin/primer3/primer3_www.cgi ) .
the pcr premix contained 20 l of 10 buffer i ( abi co. , ltd . , foster city , usa ) , 3.0 mm mgcl2 , 0.3 mm dntps , 1 unit of hotstartaq dna polymerase ( qiagen co. , ltd . ,
valencia , spain ) , 1 l of dna and 2 l of multi - pcr primer .
the pcr thermocycling conditions were : 95 c for 2 min ; 11 cycles of 94 c for 20 s , 65 c ( reduced by 0.5 c each cycle ) for 40 s , 72 c for 90 s ; followed by 24 cycles of 94 c for 20 s , 59 c for 30 s , 72 c for 90 s , and a final extension at 72 c for 2 min .
multiplex single - base primer extension reactions were performed using the snapshot multiplex kit ( abi co. , ltd .
the reactions were then treated with one unit of calf intestinal phosphatase ( new england biolabs , ma , usa ) , at 37 c for 60 min , followed by 15 min at 75 c .
labelled extension products were resolved by capillary electrophoresis , on an dna analyzer ( abi co. , ltd .
, foster city , usa ) , and data analysis was performed using genemapper analysis software , version 4.0 ( biosystems co. , ltd .
participants were divided into two groups in accordance with the ethnicity given in the baseline survey : ( a ) han nationality ( b ) uyghur nationality .
data for all variables were normally distributed , which allowed parametric tests of significance to be performed .
comparisons of the basic characteristics , bmd and serum biology , between the groups , were calculated through t - tests or t  -tests . to determine whether the allele distributions of the snps satisfied the hardy  weinberg equilibrium ,
a chi - squared test was used to compare the observed and expected allele frequencies .
the association between each snp and physical characteristics ( age , weight and height ) , in addition to dietary calcium intake , energy intake , vitamin d intake and bmd , was assessed by an analysis of variance ( anova ) .
differences in bmd and snp alleles , in relation to ethnicity , were assessed by t - tests .
regression analysis was used to determine the factors that predict bmd in the lumbar spine , evaluate their significance and assess the proportion of variance that these factors can explain .
the regression model analyzed the following variables : ethnic group , rs3762397 geno - type , rs1298989 genotype , age , bmi , age at menarche , total calcium in - take , energy intake , vitamin d intake , regular exercise , current smoking habits , current drinking habits , habitual tea consumption and habitual milk consumption .
all statistical analyses were carried out using spss for windows , version 13.0 ( spss , chicago , il , usa ) .
there were no differences in age , weight , height , bmi index , menarche and serum biology , between the han and uyghur groups .
in contrast , total calcium intake , energy intake , habitual tea consumption and habitual milk consumption were significantly different between the two groups .
basic characteristics , bone mineral densities and lifestyle factors of han and uyghur female college students analysis of the rs3762397 snp identified three possible genotypes ; cc , ct and tt . of the 203 han participants ,
62.56% had the cc genotype , 33.99% had the ct genotype and 3.45% had the tt genotype .
allele frequencies were 0.796 for the c allele and 0.204 for the t allele , which were consistent with the hardy  weinberg equilibrium ( =0.410 , p = 0.522 ) .
of the 156 uyghur participants , 36.54% had the cc genotype , 53.21% had the ct genotype and 10.26% had the tt genotype .
allele frequencies were 0.631 for the c allele and 0.369 for the t allele , which were consistent with the hardy  weinberg equilibrium ( =3.193 , p = 0.074 ) .
significant differences were observed between the han and uyghur genotype and allele frequencies ( p = 0.000 , p = 0.000 ) ( table 2 ) .
comparison of the distribution of genotypes and allele frequencies for rs3762397 between han and uyghur female college students the observed rs1298989 genotypes were ga and gg , aa was absent .
of the 203 han participants , 4.93% had the ga genotype , whilst 95.07% had the gg genotype .
allele frequencies were 0.975 for the g allele and 0.025 for the a allele , which were consistent with the hardy - weinberg equilibrium ( =0.128 , p = 0.279 ) .
of the 156 uyghur participants , 23.72% had the ga genotype , whilst 76.28% had the gg genotype .
allele frequencies were 0.881 for the g allele and 0.119 for the a allele , which satisfied the hardy  weinberg equilibrium ( =2.808 , p = 0.906 ) .
significant differences were observed between han and uyghur genotype and allele frequencies ( p = 0.000 , p = 0.000 ) ( table 3 ) .
comparison of the distribution of genotypes and allele frequencies for rs1298989 between han and uyghur female college students although age , weight , height , menarche , total calcium intake and vitamin d intake were similar across all rs3762397 and rs1298989 genotypes , in both the han and uyghur groups , the l2 - 4 bmd association with rs3762397 genotypes was different between the two groups .
the bmd values in han subjects with the tt genotype were significantly ( p < 0.05 ) higher than in those with the cc genotype .
the bmd values in uyghur subjects with the cc or tt genotype were significantly ( p < 0.05 ) higher than in those with the ct genotype .
significant differences were observed in the l2 - 4 bmd values of the uyghur group , between the rs1298989 genotypes .
the uyghur subjects with the gg genotype had significantly higher bmd values , compared with subjects with the ga genotype ( tables 4 and 5 ) .
comparison of demographic features , clinical characteristics , daily energy and nutrient intake and bmd values in the han and uyghur subjects , categorized by rs3762397 genotype comparison of demographic features , clinical characteristics , daily energy and nutrient intake and bmd values in the han and uyghur subjects , categorized by rs1298989 genotype there was no association between rs1298989 genotype and bmd in the han group ( p > 0.05 ) the allelic association with bmd was different between the two groups for the rs3762397 snp .
a high bmd was associated with the cc genotype in uyghur subjects ( p = 0.003 ) , while it was associated with the ct genotype in han subjects ( p = 0.019 ) ( table 6 ) .
bmd of rs3762397 and rs1298989 genotypes in han and uyghur female college students we also analyzed the correlation between bmd and risk factors using a multivariate linear statistical method .
the regression equation was : y(bmd ) = 76.208 + 8.465vitamm d intake + 11.904habitual milk consumption+ 9.18rs1298989 + 6.969regular exercise + 0.009energy intake .
variables associated with bmd in han and uyghur female college students , with increased bmd of the lumbar spine evaluated by multiple regression analysis the variables explained 13.4% of the variance in bmd .
the second variable , in terms of the size of impact , was habitual milk consumption .
there were no differences in age , weight , height , bmi index , menarche and serum biology , between the han and uyghur groups .
in contrast , total calcium intake , energy intake , habitual tea consumption and habitual milk consumption were significantly different between the two groups .
basic characteristics , bone mineral densities and lifestyle factors of han and uyghur female college students
analysis of the rs3762397 snp identified three possible genotypes ; cc , ct and tt .
of the 203 han participants , 62.56% had the cc genotype , 33.99% had the ct genotype and 3.45% had the tt genotype .
allele frequencies were 0.796 for the c allele and 0.204 for the t allele , which were consistent with the hardy  weinberg equilibrium ( =0.410 , p = 0.522 ) .
of the 156 uyghur participants , 36.54% had the cc genotype , 53.21% had the ct genotype and 10.26% had the tt genotype .
allele frequencies were 0.631 for the c allele and 0.369 for the t allele , which were consistent with the hardy  weinberg equilibrium ( =3.193 , p = 0.074 ) .
significant differences were observed between the han and uyghur genotype and allele frequencies ( p = 0.000 , p = 0.000 ) ( table 2 ) .
comparison of the distribution of genotypes and allele frequencies for rs3762397 between han and uyghur female college students the observed rs1298989 genotypes were ga and gg , aa was absent .
of the 203 han participants , 4.93% had the ga genotype , whilst 95.07% had the gg genotype .
allele frequencies were 0.975 for the g allele and 0.025 for the a allele , which were consistent with the hardy - weinberg equilibrium ( =0.128 , p = 0.279 ) .
of the 156 uyghur participants , 23.72% had the ga genotype , whilst 76.28% had the gg genotype .
allele frequencies were 0.881 for the g allele and 0.119 for the a allele , which satisfied the hardy  weinberg equilibrium ( =2.808 , p = 0.906 ) .
significant differences were observed between han and uyghur genotype and allele frequencies ( p = 0.000 , p = 0.000 ) ( table 3 ) .
comparison of the distribution of genotypes and allele frequencies for rs1298989 between han and uyghur female college students
although age , weight , height , menarche , total calcium intake and vitamin d intake were similar across all rs3762397 and rs1298989 genotypes , in both the han and uyghur groups , the l2 - 4 bmd association with rs3762397 genotypes was different between the two groups .
the bmd values in han subjects with the tt genotype were significantly ( p < 0.05 ) higher than in those with the cc genotype .
the bmd values in uyghur subjects with the cc or tt genotype were significantly ( p < 0.05 ) higher than in those with the ct genotype .
significant differences were observed in the l2 - 4 bmd values of the uyghur group , between the rs1298989 genotypes .
the uyghur subjects with the gg genotype had significantly higher bmd values , compared with subjects with the ga genotype ( tables 4 and 5 ) .
comparison of demographic features , clinical characteristics , daily energy and nutrient intake and bmd values in the han and uyghur subjects , categorized by rs3762397 genotype comparison of demographic features , clinical characteristics , daily energy and nutrient intake and bmd values in the han and uyghur subjects , categorized by rs1298989 genotype there was no association between rs1298989 genotype and bmd in the han group ( p > 0.05 ) the allelic association with bmd was different between the two groups for the rs3762397 snp . a high bmd was associated with the cc genotype in uyghur subjects ( p = 0.003 ) , while it was associated with the ct genotype in han subjects ( p = 0.019 ) ( table 6 ) .
we also analyzed the correlation between bmd and risk factors using a multivariate linear statistical method .
the regression equation was : y(bmd ) = 76.208 + 8.465vitamm d intake + 11.904habitual milk consumption+ 9.18rs1298989 + 6.969regular exercise + 0.009energy intake .
variables associated with bmd in han and uyghur female college students , with increased bmd of the lumbar spine evaluated by multiple regression analysis the variables explained 13.4% of the variance in bmd .
the second variable , in terms of the size of impact , was habitual milk consumption .
in this study , we observed differences in genotype and allele frequencies of the rs1298989 snp in the catsperb gene and rs3762397 snp in the nr5a2 gene , between female han and uyghur college students .
differences in genotype distributions were observed , between the han and uyghur participants , for both snps .
this was consistent with other studies that have shown population differences in the distribution of genotypes ( 16 , 17 ) , which were consistent with the hardy - weinberg equilibrium within a population .
the aa genotype of the apa i snp in the vitamin d receptor ( vdr ) gene was found in 7.8% of chinese han women ( 18 ) , 15.1% of japanese women ( 19 ) , 36% of an indian population ( 20 ) .
anthropological research has demonstrated that the gene structure of uyghur subjects contains caucasian and mongolian ancestry ( 21 , 22 ) .
these gene fusion characteristics may explain the differences observed in the genotype distributions and bmd phenotype , when compared with the han chinese .
bmd is related to hormonal balance , aging , environmental factors , life style and genetic predisposition .
familial and twin studies have estimated that 6080% of the variance in pbmd is due to genetic factors ( 23 ) .
our investigation showed that adequate vitamin d intake , drinking milk , rs1298989 genotype , regular exercise and adequate energy intake were factors that can be used to predict increased bmd in the lumbar spine , in female college students .
although the genotype of rs1298989 and rs3762397 affected the pbmd , they did not show a large effect .
it may be due to existence of multiple genes and their interactions effect on pbmd ( 3 , 24 ) .
our research showed that the factor with the greatest impact on bmd in the lumbar spine was vitamin d intake .
vitamin d is a secosteroid hormone that is essential for calcium absorption and bone mineralization , which are positively associated with bmd .
a number of studies have investigated the relationship between adequate calcium intake and bmd ( 25 , 26 ) .
vitamin d deficiency leads to decreased calcium absorption and ultimately the release of calcium from the bones , in order to maintain circulating calcium concentrations ( 27 ) .
uyghur is the main minority group and the ethnic culture , religion , lifestyle and dietary customs are different from those of the han people .
the uyghur diet consists mainly of meat ( lamb and beef ) , milk , cooked wheaten food and dairy products .
it is high in calories , fat , spices , strong flavors , strong and milky tea ( milky tea is the conventional drink of the uyghur ) .
in contrast , the han chinese population in the same region eats mainly vegetables , fish , pork , eggs and rice .
this study showed that total calcium intake , energy intake , habitual tea consumption and habitual milk consumption were significantly different between the han and uyghur populations .
our data showed that the second variable , in terms of impact on bmd in the lumbar spine , was the habit of drinking milk .
the presence of minerals and macronutrients in milk , in addition to calcium , may promote bone mineralization in adolescent girls or young women .
studies have shown that adequate calcium intake during childhood and adolescence is vital for bmd ( 28 , 29 ) .
( 28 ) investigated 192 unrelated , healthy adolescent girls and young women and found that milk consumption , in preference to other calcium sources , is associated with lumbar bone mineral content ( bmc ) and bmd .
exercise that involves the loading of bone also has a major impact on bone mass ( 30 , 31 ) .
( 32 ) showed that increased physical activity is related to an advantageous development of bmd and cortical bone size , which indicated that exercise is important for the optimization of pbmd in young men .
however , there was no statistical difference in the levels of regular exercise between han and uyghur subjects .
this caused the uyghur subjects to use more energy than the han subjects , who are relatively quiet , introverted and like to play badminton , walk and do yoga .
firstly , there are more daylight hours in xinjiang , than hours of darkness , and cold , dry conditions are the main climatic features that differ from inland areas .
vitamin d plays an important role in skeletal development and maintenance , and it is generally accepted that serum 25(oh ) d reflects vitamin d status .
healthy adults obtain most of their vitamin d through exposure of skin to sunlight , with natural dietary sources being limited ( 33 , 34 ) .
the levels of sunshine and 25(oh ) d levels were not measured in this study .
we did not collect familial information or analyze the relevant factors , especially the information of their mothers , it should be estimated in the further study .
in addition , not all potential confounding factors , such as estrogen hormone , were assessed and measured .
this study showed that the rs1298989 polymorphism of the catsperb gene and rs3762397 polymorphism of the nr5a2 gene were associated with pbmd in han and uyghur populations .
it also demonstrated the correlations between bmd and variables such as adequate vitamin d intake , drinking milk , genotype , regular exercise and adequate energy intake .
these variables were the main predictors of increased bmd in the lumbar spine of female college students .
ethical issues ( including plagiarism , informed consent , misconduct , data fabrication and/or falsification , double publication and/or submission , redundancy , etc . ) have been completely observed by the authors .
 abstractbackgroundpeak bone mineral density ( pbmd ) is influenced by both genetic and environmental factors , genes explains most of variation . 
 as the novel candidate genes catsperb and nr5a2 may have been associated with spinal pbmd in adult . 
 this study was to investigate the relationship among these two genes^ pbmd and the life style factors in young female.methodsthe rs1298989 single nucleotide polymorphism ( snp ) of the catsperb gene and the rs3762397 snp of the nr5a2 gene were genotyped using snapshot in 359 students from xinjiang . 
 the prospective study included 203 han and 156 uyghur subjects . 
 pbmd was measured using quantitative computed tomography ( qct ) . 
 calcium , phosphate and alkaline phosphatase were measured by elisa method . 
 physical activity , dietary calcium and life styles were assessed by questionnaire.resultsboth snps showed differences in genotype and allele frequencies ( p < 0.05 ) between the han and uyghur subjects . 
 total calcium intake , energy intake , tea and milk intake were also significantly different between two groups ( p < 0.05 ) . 
 multiple regression analysis showed an association between pbmd and vitamin d intake ( p = 0.000 ) , milk ( p = 0.000 ) , exercise ( p = 0.029 ) , rs1298989 ( p = 0.028 ) , energy intake ( p = 0.043).conclusionthis study demonstrated the polymorphisms of the rs1298989 and rs3762397 are associated with pbmd both in han and uyghur subjects . 
 pbmd , in xinjiang , appears to be associated with several known factors that are well described in the literature . while the genotypes of rs1298989 and rs3762397 do not appear have a strong effect on the pbmd 
 . 
Introduction
Materials and Methods
Materials
Questionnaire
Measurement
Assessment of dietary calcium and vitamin D
Genotyping test
Statistical analysis
Results
Comparison the basic characteristics, bone mineral densities and lifestyle factors
Comparison of the distribution of genotypes and allele frequencies for rs3762397 and rs1298989
Comparison of demographic features, clinical characteristics, daily energy and nutrient intake and BMD values
Variables associated with BMD
Discussion
Conclusion
Ethical considerations