risk factors for the development and progression of glaucoma include elevated intraocular pressure ( iop ) , older age , advanced glaucoma stage , lower central corneal thickness , decreased ocular perfusion pressure , and the presence of disc hemorrhage ( dh ) .
the structural features of the lamina cribrosa ( lc ) were recently investigated as potential risk factors for glaucoma progression .
eyes with lc defects tended to progress faster than eyes without lc defects . a thinner , deeper lc
was significantly associated with the rate of progressive retinal nerve fiber layer ( rnfl ) thinning .
posterior lc displacement was prominent in preperimetric and mild - to - moderate glaucoma when different glaucoma stages were compared .
however , the roles of the lc findings and how they may contribute to glaucoma progression remain to be determined . with the advent of spectral - domain optical coherence tomography ( oct ) with enhanced depth imaging ( edi ) ,
park proposed that structural characteristics of the lc in patients with glaucoma can occur in both generalized and localized patterns .
the lc depth ( lcd ) and lc thickness ( lct ) , which could be one of generalized characteristics of the lc , are reported to be associated with age and iop .
focal lc defects , which could be one of focal characteristics of the lc , are reported to be associated with localized rnfl defects , dh , normal - tension glaucoma diagnosis , and myopic refractive errors .
because the related factors differ between generalized and focal lc characteristics , we hypothesized that these characteristics may have different roles in glaucoma progression .
the purpose of this study was to determine the role of generalized lc characteristics , as assessed by lcd and lct , and focal lc characteristics , as assessed by the presence of focal lc defects , on the future rate of progressive rnfl thinning in patients with glaucoma .
mary 's hospital , seoul , south korea and followed the tenets of the declaration of helsinki .
written informed consent was obtained from consecutive patients who met the eligibility criteria and were willing to participate in the study .
these patients were part of the cmc suspect cohort study , an ongoing study that has been conducted since march 2009 .
patients who had undergone at least 4 oct examinations ( cirrus oct ; carl zeiss meditec , inc . ,
dublin , ca ) with follow - up at for least 3 years were selected for this study .
each participant underwent a comprehensive ophthalmic assessment , including measurement of best - corrected visual acuity , refraction , slit - lamp biomicroscopy , gonioscopy , goldmann applanation tonometry , central corneal thickness using ultrasound pachymetry ( tomey corporation , nagoya , japan ) , determination of axial length using ocular biometry ( iol master ; carl zeiss meditec , inc . ,
dublin , ca ) , dilated stereoscopic examination of the optic disc and fundus , color disc photography , red - free rnfl photography ( canon , tokyo , japan ) , and humphrey vf examination using the 24 - 2 swedish interactive threshold algorithm standard program ( carl zeiss meditec , inc . ,
all patients were followed every 3 to 6 months with regular follow - up , including slit - lamp biomicroscopy , goldmann applanation tonometry , and optic disc examination for dh detection .
color disc and fundus photography and cirrus oct examinations were usually repeated at intervals ranging from 6 to 12 months . for a glaucoma diagnosis
, patients had to fulfill the following criteria : glaucomatous optic disc appearances ( such as diffuse or localized rim thinning , a notch in the rim , or a vertical cup - to - disc ratio higher than that of the other eye by > 0.2 ) and glaucomatous vf loss ( defined as the consistent presence of a cluster of 3 nonedge points on the pattern deviation plot with a probability of occurring in < 5% of the normal population , with one of these points having the probability of occurring in < 1% of the normal population , a pattern standard deviation with p  <  5% , or a glaucoma hemifield test result outside the normal limits in a consistent pattern on 2 qualifying vfs ) , confirmed by 2 glaucoma specialists ( hyp and ckp ) , in addition to an open angle on gonioscopic examination . to be included in the study ,
patients were required to meet the following inclusion criteria : a best - corrected visual acuity of 20/40 , a spherical refraction within 6.0 diopters , a cylinder correction within 3.0 diopters , consistently reliable vfs ( defined as false - negative rate of < 15% , false - positive rate of < 15% , and fixation losses of < 20% ) , and a mean deviation worse than 30.00  db .
patients were excluded on the basis of any of the following criteria : a history of any retinal diseases ; a history of eye trauma or surgery , with the exception of uncomplicated cataract surgery ; a glaucoma incisional surgery or laser procedure ; another optic nerve disease besides glaucoma ; or a history of systemic or neurological diseases that might affect the vf .
one eye was randomly selected from each patient with glaucoma who met the inclusion and exclusion criteria if both eyes were eligible .
we recorded the age at the time of edi oct and baseline vf mean deviation at the initial visit .
the mean iop during the entire follow - up period was calculated by averaging all measurements .
dh was defined as an isolated flame - shape or splinter - like hemorrhage on the optic disc or peripapillary area , extending to the border of the optic disc .
stereoscopic disc photographs and medical records were reviewed for the presence of dh . at the time of enrollment , serial horizontal and vertical cross - sectional scans covering the optic nerve head ( onh ) ,
approximately 30  m apart , were obtained using the edi technique of the spectralis oct ( heidelberg engineering , gmbh , dossenheim , germany ) for lc analyses , as described previously .
images with a quality score > 15 were obtained ( approximately 6570 sections per eye ) .
first , the edi oct images were reviewed for the presence of focal lc defects that violated the smooth curvilinear u- or w - shaped cross - sectional contour of the lc .
experienced glaucoma specialists ( hyp and sik ) , blinded to the patients clinical information and infrared optic disc photographs , performed this review .
focal characteristics of the lc were defined using the guidelines specified by kiumehr et al focal lc defects were considered to be present when violating the curvilinear u- or w - shaped contour of the anterior lc surface was observed .
the diameter of the defects at their opening was required to be 100  m in diameter and > 30  m in depth .
in addition , to be considered as focal lc defects , the lc findings had to be present in 2 neighboring b - scans to avoid false positives in both the horizontal and vertical scans ( fig .
the location of the focal lc defect was classified into superior or inferior region according to the location of the b - scan in which the defect was found . to assess the intraobserver reproducibility of focal lc defect evaluation , edi oct images of 50 randomly selected glaucomatous eyes were evaluated .
the absolute agreement of the single observer 's evaluation was calculated with the intraclass correlation coefficient from a 2-way mixed effects model .
enhanced depth imaging of the optic nerve head using spectralis optical coherence tomography was performed at the time of enrollment .
a focal lamina cribrosa ( lc ) defect was considered as a violation of the curvilinear u- or w - shaped contour of the anterior lc surface ( green outline ) . it had to be identified in 2 neighboring b - scans with their opening to be 100  m in diameter and > 30  m in depth .
both lc depth ( lcd ) and lc thickness ( lct ) were measured at three locations ( superior midperipheral , green arrow ; midhorizontal , blue arrow ; inferior midperipheral , yellow arrow ) .
the lcd was determined by measuring the distance from the bruch membrane ( bm ) opening plane to the level of the anterior lc surface .
the reference line ( red line ) connecting the 2 termination points of the bm edges ( red glyphs ) was used as a reference plane and was drawn in each b - scan image . the distance from the reference line to the level of the anterior border of the lc was measured at 3 points in each location ( the maximally depressed point and 2 additional points , 100 and 200  m temporally from the maximally depressed point ) .
the average of the 3 values was used as the lcd ( three red perpendicular lines ) at that location .
the lct was measured as the distance between the anterior and posterior borders of lc in the direction perpendicular to the anterior lc surface at the measurement point ( yellow line between yellow glyphs marking the anterior and posterior border of the lc ) .
the average of 3 values from 3 locations was used as the mean lcd and mean lct .
next , the lcd and lct were measured at 3 locations ( midhorizontal , superior , and inferior midperipheral regions of the onh ) .
the lcd was determined by measuring the distance from the opening plane of bruch membrane ( bm ) to the level of the anterior lc surface .
the reference line connecting the 2 termination points of the bm edges was used as a reference plane and was drawn in each b - scan image ( fig . 1 , red glyphs and red line ) .
the distance from the reference line to the level of the anterior border of the lc was measured at 3 points ( the maximally depressed point and 2 additional points , 100 and 200  m temporally from the maximally depressed point ) .
the average of the 9 measurements , 3 values from each 3 location , was used as the lcd ( fig . 1 , 3 red perpendicular lines ) .
the lct was measured as the distance between the anterior and posterior borders of the lc in the direction perpendicular to the anterior lc surface at the measurement point ( fig .
1 , yellow line between yellow glyphs marking the anterior and posterior borders of the lc ) .
the measurement of the lcd and lct were classified into superior and inferior lcd / lct according to the measured location .
average of 3 measurements for lcd and 1 measurement of lct was considered as superior and inferior lcd / lct . to assess intraobserver reproducibility of the measurement of lcd and lct , edi oct images of 100
the absolute agreement of the single observer 's evaluation was calculated with the intraclass correlation coefficient from 2-way mixed effects model .
rnfl thickness was measured using the optic disc cube protocol of a cirrus oct running version 6.0 software ( cirrus oct ; carl zeiss meditec , inc . ,
the optic disc cube protocol scans a 6    6  mm area centered on the onh and collects 200    200 axial scans containing 40,000 points .
images exhibiting involuntary saccade , misalignment , or blinking artifacts and those with a signal strength of < 6 were discarded .
eyes were classified as those with or without progressive rnfl thinning using the guided progression analysis ( gpa ) software of cirrus oct .
at least 4 oct examinations are necessary to generate a gpa report , which was an inclusion criterion for this study . the superior and inferior rnfl thicknesses are plotted against the duration of follow - up .
if progression was suspected , the rnfl thickness at each visit was indicated as  possible loss  or  likely loss . 
progression was identified if the observed change from baseline ( the first 2 oct examinations ) to a test value exceeds the test  retest variability of the system . if the   possible loss 
criterion was met on 2 successive visits , that patient was considered to show  likely loss .
likely loss  in either the superior or inferior region reflected progressive rnfl thinning and thus glaucomatous progression .
linear regression analysis against time was performed for the global average , inferior , and superior rnfl thicknesses for each patient to determine the rate of change in the rnfl thickness ( expressed in m / year ) .
a general linear model was used to identify factors associated with the rate of change in rnfl thickness .
the dependent variable was the rate of rnfl thinning in the global , inferior , and superior regions .
the independent variables exhibiting significance at p  <  0.10 in the univariate model were included in the multivariate model . a p value of  <  0.05 was considered to indicate statistical significance .
statistical analyses were performed using the spss software ( ver . 16.0 ; spss inc . ,
mary 's hospital , seoul , south korea and followed the tenets of the declaration of helsinki .
written informed consent was obtained from consecutive patients who met the eligibility criteria and were willing to participate in the study .
these patients were part of the cmc suspect cohort study , an ongoing study that has been conducted since march 2009 .
patients who had undergone at least 4 oct examinations ( cirrus oct ; carl zeiss meditec , inc . ,
dublin , ca ) with follow - up at for least 3 years were selected for this study .
each participant underwent a comprehensive ophthalmic assessment , including measurement of best - corrected visual acuity , refraction , slit - lamp biomicroscopy , gonioscopy , goldmann applanation tonometry , central corneal thickness using ultrasound pachymetry ( tomey corporation , nagoya , japan ) , determination of axial length using ocular biometry ( iol master ; carl zeiss meditec , inc . ,
dublin , ca ) , dilated stereoscopic examination of the optic disc and fundus , color disc photography , red - free rnfl photography ( canon , tokyo , japan ) , and humphrey vf examination using the 24 - 2 swedish interactive threshold algorithm standard program ( carl zeiss meditec , inc . ,
all patients were followed every 3 to 6 months with regular follow - up , including slit - lamp biomicroscopy , goldmann applanation tonometry , and optic disc examination for dh detection .
color disc and fundus photography and cirrus oct examinations were usually repeated at intervals ranging from 6 to 12 months . for a glaucoma diagnosis
, patients had to fulfill the following criteria : glaucomatous optic disc appearances ( such as diffuse or localized rim thinning , a notch in the rim , or a vertical cup - to - disc ratio higher than that of the other eye by > 0.2 ) and glaucomatous vf loss ( defined as the consistent presence of a cluster of 3 nonedge points on the pattern deviation plot with a probability of occurring in < 5% of the normal population , with one of these points having the probability of occurring in < 1% of the normal population , a pattern standard deviation with p  <  5% , or a glaucoma hemifield test result outside the normal limits in a consistent pattern on 2 qualifying vfs ) , confirmed by 2 glaucoma specialists ( hyp and ckp ) , in addition to an open angle on gonioscopic examination . to be included in the study ,
patients were required to meet the following inclusion criteria : a best - corrected visual acuity of 20/40 , a spherical refraction within 6.0 diopters , a cylinder correction within 3.0 diopters , consistently reliable vfs ( defined as false - negative rate of < 15% , false - positive rate of < 15% , and fixation losses of < 20% ) , and a mean deviation worse than 30.00  db .
patients were excluded on the basis of any of the following criteria : a history of any retinal diseases ; a history of eye trauma or surgery , with the exception of uncomplicated cataract surgery ; a glaucoma incisional surgery or laser procedure ; another optic nerve disease besides glaucoma ; or a history of systemic or neurological diseases that might affect the vf .
one eye was randomly selected from each patient with glaucoma who met the inclusion and exclusion criteria if both eyes were eligible .
we recorded the age at the time of edi oct and baseline vf mean deviation at the initial visit .
the mean iop during the entire follow - up period was calculated by averaging all measurements .
dh was defined as an isolated flame - shape or splinter - like hemorrhage on the optic disc or peripapillary area , extending to the border of the optic disc .
at the time of enrollment , serial horizontal and vertical cross - sectional scans covering the optic nerve head ( onh ) , approximately 30  m apart , were obtained using the edi technique of the spectralis oct ( heidelberg engineering , gmbh , dossenheim , germany ) for lc analyses , as described previously .
images with a quality score > 15 were obtained ( approximately 6570 sections per eye ) .
first , the edi oct images were reviewed for the presence of focal lc defects that violated the smooth curvilinear u- or w - shaped cross - sectional contour of the lc .
experienced glaucoma specialists ( hyp and sik ) , blinded to the patients clinical information and infrared optic disc photographs , performed this review .
focal characteristics of the lc were defined using the guidelines specified by kiumehr et al focal lc defects were considered to be present when violating the curvilinear u- or w - shaped contour of the anterior lc surface was observed .
the diameter of the defects at their opening was required to be 100  m in diameter and > 30  m in depth .
in addition , to be considered as focal lc defects , the lc findings had to be present in 2 neighboring b - scans to avoid false positives in both the horizontal and vertical scans ( fig .
the location of the focal lc defect was classified into superior or inferior region according to the location of the b - scan in which the defect was found . to assess the intraobserver reproducibility of focal lc defect evaluation , edi oct images of 50 randomly selected glaucomatous eyes were evaluated .
the absolute agreement of the single observer 's evaluation was calculated with the intraclass correlation coefficient from a 2-way mixed effects model .
enhanced depth imaging of the optic nerve head using spectralis optical coherence tomography was performed at the time of enrollment . a focal lamina cribrosa ( lc )
defect was considered as a violation of the curvilinear u- or w - shaped contour of the anterior lc surface ( green outline ) .
it had to be identified in 2 neighboring b - scans with their opening to be 100  m in diameter and > 30  m in depth . both lc depth ( lcd ) and lc thickness ( lct ) were measured at three locations ( superior midperipheral , green arrow ; midhorizontal , blue arrow ; inferior midperipheral , yellow arrow ) .
the lcd was determined by measuring the distance from the bruch membrane ( bm ) opening plane to the level of the anterior lc surface .
the reference line ( red line ) connecting the 2 termination points of the bm edges ( red glyphs ) was used as a reference plane and was drawn in each b - scan image . the distance from the reference line to the level of the anterior border of the lc was measured at 3 points in each location ( the maximally depressed point and 2 additional points , 100 and 200  m temporally from the maximally depressed point ) .
the average of the 3 values was used as the lcd ( three red perpendicular lines ) at that location .
the lct was measured as the distance between the anterior and posterior borders of lc in the direction perpendicular to the anterior lc surface at the measurement point ( yellow line between yellow glyphs marking the anterior and posterior border of the lc ) .
the average of 3 values from 3 locations was used as the mean lcd and mean lct .
next , the lcd and lct were measured at 3 locations ( midhorizontal , superior , and inferior midperipheral regions of the onh ) .
the lcd was determined by measuring the distance from the opening plane of bruch membrane ( bm ) to the level of the anterior lc surface .
the reference line connecting the 2 termination points of the bm edges was used as a reference plane and was drawn in each b - scan image ( fig . 1 , red glyphs and red line ) .
the distance from the reference line to the level of the anterior border of the lc was measured at 3 points ( the maximally depressed point and 2 additional points , 100 and 200  m temporally from the maximally depressed point ) .
the average of the 9 measurements , 3 values from each 3 location , was used as the lcd ( fig . 1 , 3 red perpendicular lines ) .
the lct was measured as the distance between the anterior and posterior borders of the lc in the direction perpendicular to the anterior lc surface at the measurement point ( fig .
1 , yellow line between yellow glyphs marking the anterior and posterior borders of the lc ) .
the measurement of the lcd and lct were classified into superior and inferior lcd / lct according to the measured location .
average of 3 measurements for lcd and 1 measurement of lct was considered as superior and inferior lcd / lct . to assess intraobserver reproducibility of the measurement of lcd and lct , edi oct images of 100
the absolute agreement of the single observer 's evaluation was calculated with the intraclass correlation coefficient from 2-way mixed effects model .
rnfl thickness was measured using the optic disc cube protocol of a cirrus oct running version 6.0 software ( cirrus oct ; carl zeiss meditec , inc . , dublin , ca ) .
the optic disc cube protocol scans a 6    6  mm area centered on the onh and collects 200    200 axial scans containing 40,000 points .
images exhibiting involuntary saccade , misalignment , or blinking artifacts and those with a signal strength of < 6 were discarded .
eyes were classified as those with or without progressive rnfl thinning using the guided progression analysis ( gpa ) software of cirrus oct .
at least 4 oct examinations are necessary to generate a gpa report , which was an inclusion criterion for this study .
the superior and inferior rnfl thicknesses are plotted against the duration of follow - up .
if progression was suspected , the rnfl thickness at each visit was indicated as  possible loss  or  likely loss . 
progression was identified if the observed change from baseline ( the first 2 oct examinations ) to a test value exceeds the test  retest variability of the system . if the   possible loss 
criterion was met on 2 successive visits , that patient was considered to show  likely loss .  we considered that  likely loss  in either the superior or inferior region reflected progressive rnfl thinning and thus glaucomatous progression .
linear regression analysis against time was performed for the global average , inferior , and superior rnfl thicknesses for each patient to determine the rate of change in the rnfl thickness ( expressed in m / year ) .
a general linear model was used to identify factors associated with the rate of change in rnfl thickness .
the dependent variable was the rate of rnfl thinning in the global , inferior , and superior regions .
the independent variables exhibiting significance at p  <  0.10 in the univariate model were included in the multivariate model . a p value of  <  0.05 was considered to indicate statistical significance .
in total , 127 eyes of 127 patients with open - angle glaucoma who met the inclusion and exclusion criteria were included .
of these 127 eyes , 9 ( 7.1% ) were excluded from further analysis because of poor oct scan images of the lc or unable to measure the lc thickness .
all 118 eyes were under treatment to lower the iop ( 72.8% on prostaglandins ) .
the mean number of evaluated octs was 5.87    0.70 , with a mean follow - up of 4.18    0.79 years .
focal lc defects were present in 66 of 118 ( 55.9% ) eyes . among these focal lc defects , 29 ( 24.6% ) eyes
were located in the superior region and 37 ( 31.4% ) in the inferior region .
the mean lcd and lct were 425.35    138.50 ( range , 146914 ) m and 242.05    46.14 ( range , 150410 ) m , respectively .
detection of the presence of focal lc defects and measurements of lcd and lct showed excellent intraobserver reproducibility .
the intraclass correlation coefficient for focal lc defect evaluation was 0.973 [ 95% confidence interval ( 95% ci )  =  0.9620.985 ] , 0.960 ( 95%
the global rate of rnfl thinning in all eyes was 0.88    2.48 ( range , 6.0 to 3.5 ) m / year .
the rate of rnfl thinning was 1.73    4.09 ( range , 11.0 to 2.2 ) m / year in the superior region and 2.14    3.04 ( range , 16.5 to 2.0 ) m / year in the inferior region .
factors associated with the rate of superior and inferior rnfl thinning were evaluated using a general linear model .
when factors associated with the rate of superior rnfl thinning were evaluated , a greater baseline lcd and thinner baseline lct were the only factors significantly associated in both univariate and multivariate analyses ( table 2 ) .
when factors associated with the rate of inferior rnfl thinning were evaluated , a greater baseline pattern standard deviation of the vf , dh during follow - up , and the presence of focal lc defects showed statistically significant relationships in the univariate analysis .
the presence of dh during follow - up and the presence of focal lc defects at baseline were significantly associated with inferior rnfl thinning in the multivariate analysis ( table 3 ) .
factors associated with the rate of inferior retinal nerve fiber layer thinning . a scatterplot indicating the relationship between the rate of rnfl thinning and the lc parameters ( mean lcd and lct ) is shown in fig .
the rate of global rnfl thinning showed a significant linear relationship with the lcd ( r  =  0.037 , p  =  0.038 ) and lct ( r  =  0.085 , p  =  0.001 ) .
however , the relationship between the lcd and rate of superior rnfl thinning was stronger ( r  =  0.273 , p  <  0.001 ) .
there was no significant relationship between the lc parameters and the rate of inferior rnfl thinning .
there was no significant relationship between the lc parameters and the rate of inferior rnfl thinning .
the relationship with the rate of inferior rnfl thinning in eyes without focal lc defects showed that a deeper lcd and thinner lct were related to more rapid rnfl thinning , although the relationship was statistically significant only with the lct .
however , in eyes with focal lc defects , the rate of inferior rnfl thinning showed no relationship with the lcd or lct .
scatterplot showing the relationship between the rate of retinal nerve fiber layer ( rnfl ) thinning and the lamina cribrosa depth ( lcd ) and the lamina cribrosa thickness ( lct ) .
the relationships with global rnfl thinning ( left ) , superior rnfl thinning ( middle ) , and inferior rnfl thinning ( right ) are shown .
scatterplot showing the relationship between the rate of inferior retinal nerve fiber layer thinning and the lamina cribrosa ( lc ) depth or the lc thickness in eyes with and without focal lc defects .
eyes were classified into progressive or nonprogressive rnfl thinning groups using the gpa analysis of the cirrus oct .
eyes with progressive superior rnfl thinning showed significant differences in the superior lcd and superior lct ( p  =  0.005 and p  =  0.001 ) compared with eyes without superior rnfl progression .
the frequency of focal lc defects or the superiorly located focal lc defects did not differ between eyes with or without progressive superior rnfl thinning ( p  =  0.216 and p  =  0.537 ) .
however , eyes with progressive inferior rnfl thinning , focal lc defects were significantly more frequent in eyes with progressive inferior rnfl thinning ( 93.8% ) than in eyes without progressive inferior rnfl thinning ( 50.0% , p  =  0.001 ) .
our study demonstrated an association between lc features and the rate of rnfl thinning in patients with glaucoma .
importantly , deepening and thinning of the lc were risk factors for faster progression of global rnfl thinning , as reported previously .
a deeper and thinner lc was significantly correlated to the rate of superior rnfl thinning . for the inferior region ,
the presence of a focal lc defect was the only lc feature that was correlated to the rate of rnfl thinning .
the frequency of focal lc defects was significantly different between eyes with and without progressive inferior rnfl thinning , but was not different between eyes with and without progressive superior rnfl thinning .
these findings showed that lc features contribute differently to glaucoma progression throughout the onh region .
however , when focal lc features are present , especially in the inferior region of the lc , rnfl thinning mostly occurs in that susceptible region , whereas general features of the lc , such as deeper and thinner lc , contribute less to the rate of rnfl thinning in eyes with focal lc defects . in eyes without focal lc defects , a deeper lcd and thinner
lct had relationships with the rate of inferior rnfl thinning similar to those of global and superior rnfl thinning . in eyes with focal lc defects , other parameters such as
the lc defect size or depth may contribute to the rate of rnfl thinning ; however , this was difficult to analyze because no standardized method with which to quantify lc defects has yet been established .
previously , 100  m deepening of mean lcd increased 35% of global rnfl thinning rate .
also , 100  m thickening of mean lct decreased 85% of global rnfl thinning rate .
our data showed that 1  m deepening of mean lcd was associated with 0.7% increase and 1  m thickening of mean lct was associated with 0.6% decrease in the rate of superior rnfl thinning .
a previous study showed that eyes with vf progression showed a more frequent presence of focal lc defects .
this study showed that eyes with focal lc defects showed faster rates of both global and localized vf progression .
the inferotemporal and superotemporal regions of the lc have larger pores with less connective tissue , increasing the likelihood of glaucomatous damage .
because glaucomatous damage occurs more often in the inferotemporal and superotemporal regions , focal lc features observed in oct images have been frequently identified in these regions .
factors related to the rate of inferior rnfl thinning were the presence of dh and focal lc defects , which were also related to each other in other studies . because focal lc defects are associated with more rapid localized vf progression and progressive inferior rnfl thinning , focal lc defects may indicate regional susceptibility of the lc .
when a focal lc defect is present at baseline , localized changes in the vf and rnfl should be monitored more carefully .
the reason why only the inferior region was associated with the presence of focal lc defects and not the superior region remains unclear .
some reports have suggested that the inferotemporal lc has less connective tissue and the larger pores than does the superotemporal lc and this contributes to the increased glaucoma susceptibility in the inferior region , followed by the superior disc region .
hood et al proposed that the inferior foveal location , relative to the optic disc , results in a distinct asymmetric distribution of the circumpapillary retinal nerve fiber layer between the superior and inferior retina .
particularly ,  crowding  of the papillomacular bundles at the inferotemporal disc at the level of the lc occurs and increases susceptibility to glaucomatous damage .
focal lc defects in the inferotemporal region could add susceptibility in that region where retinal ganglion cell axons are susceptible due to a  crowding effect .  however , generalized characteristics of the lc affect the rate of rnfl thinning globally and in the superior region .
the lc demonstrates posterior bowing and compression during glaucomatous cupping . by in vivo imaging of the lc using oct ,
backward bowing could be observed as deepening of the lcd and compression as thinning of the lct .
the lcd and lct , which could be generalized lc features , are reportedly associated with age and iop .
the lcd correlated with baseline untreated iop and lcd reduction was related to the degree of iop reduction in several studies .
this could be summarized as follows : generalized lc features are mainly influenced by risk factors , such as iop and age , which have been reported to be major risk factors for global glaucoma progression . when eyes have no focal lc features by lc imaging , the lcd and lct can be used as significant risk factors to predict progressive rnfl thinning in the global and superior region .
the reason for the significant association of the lcd and lct parameter for only the superior region in terms of rnlf thinning , not in the inferior region , may be a result of the anatomy of the lc .
the anterior lc insertion is reported to be deeper in the superior than inferior region in both glaucoma and normal control eyes .
this lc insertion was shown to be located more posteriorly in glaucomatous eyes , suggesting posterior migration of the lc insertion , and it was more prominent in the superior than inferior region .
computer modeling studies have shown that more deeply located lc is exposed to increased strain within the tissue and is thought to be more susceptible to glaucomatous changes .
this could be one reason for the contribution of the lcd and lct only to superior rnlf thinning .
open - angle glaucoma with high iop is reported to exhibit diffuse damage in the rnfl and vf compared with glaucoma with normal iop .
these findings suggest that the backward bowing and posterior location of the lc by high iop may be related to the pathogenic mechanism of diffuse glaucomatous damage in glaucoma with high iop .
glaucoma with normal iop displays more localized and deeper damage in the superior vf region than does glaucoma with high iop . the progression pattern of glaucoma with normal iop
is reported to be faster in the superior vf region , consistent with our findings that eyes with focal lc defects showed faster inferior rnfl thinning .
first , only patients from a single ethnic group with glaucoma who were treated medically were included .
further investigation is needed to determine the long - term influence of lc features on glaucoma progression .
third , issues of the poor visualization of the lc under the optic disc rim and vessels exist .
it is possible that some lc alterations in areas with poor oct penetration may have been missed . to reduce false - positive detection of focal lc defects , we defined a lc defect as having a diameter of 100  m and a depth of > 30  m . the lc defect also had to be present in 2 neighboring b - scans .
the definition of lc defects was based on previous studies and may not be ideal .
however , previous studies have mentioned that the definition we used may exclude normal anatomical variations and artifacts . in conclusion
, our study shows that different lc features had different effects on regional rnfl thinning .
generalized characteristics of the lc , such as deeper and thinner lc at baseline , were related to future superior rnfl thinning .
focal characteristics of the lc , such as focal lc defects , were related to inferior rnfl thinning .
 abstractthe advent of optical coherence tomography ( oct ) imaging allows identification of the structural contribution of the lamina cribrosa ( lc ) to glaucoma progression . this study aimed to determine the role of various lc features , such as the lc depth ( lcd ) , lc thickness ( lct ) , and focal lc defects , on the future rate of progressive retinal nerve fiber layer ( rnfl ) thinning in patients with glaucoma . 
 one hundred eighteen patients with glaucoma who had undergone at least 4 oct examinations were included . 
 features of lc , including the lcd , lct , and presence of focal lc defects , from serial scan of the optic disc using the enhanced depth imaging of spectralis oct ; were analyzed at baseline . 
 eyes were classified as those with or without progressive rnfl thinning using the guided progression analysis of cirrus oct . 
 factors associated with the rate of rnfl thinning ( linear regression analysis against time for global average , inferior , and superior rnfl thicknesses , m / year ) were evaluated using a general linear model . 
 greater baseline lcd and thinner baseline lct were significantly associated with the rate of superior rnfl thinning . 
 focal lc defects were significantly more frequent in eyes with progressive inferior rnfl thinning ( 93.8% ) and the location of the focal lc defect was only related to the location of progression rnfl thinning in the inferior region ( p  <  0.001 ) . 
 a deeper and thinner lc was related to the rate of superior rnfl thinning , and the presence of focal lc defects was related to the rate of inferior rnfl thinning . 
Introduction
Methods
Study subjects
Enhanced-depth imaging optical coherence tomography
Cirrrus optical coherence tomography
Statistical analysis
Results
Discussion