this was a randomized clinical trial study that conducted in alzahra hospital in isfahan between 2004 - 2007 .
presented study consisted of 11 male patients with the mean age of 31.09 years ( ranged 20 to 44 years ) ; of which 90.9 % ( 10 patients ) had the chondral lesion in the medial condyle and 9.1 % ( one patient ) had the chondral lesion in lateral condyle of the femur .
patients presented themselves with isolated symptomatic cartilage defects , a limited chondral lesion ( mean surface area : 5 cm ) and without generalized osteoarthritis , genu valgum and/or genu varum of the knee .
patients under 40 years old with chief compliant of knee pain and up to three cartilaginous lesions between 2 - 9 cm were entered to study and patients lost to follow - up were excluded .
follow - up of all patients was for at least 12 months after surgery , additionally follow - up for 4 patients continued for 48 months .
the study was approved by the scientific & ethical committee of isfahan university of medical science ( iums ) , isfahan , iran ( no.186012 ) .
after decision for performing act was taken , approximately , a 200 - 300 mg full thickness cartilage biopsy from a healthy area of the joint was obtained from the outer edge of the superior medial condyle , from a non - weight - bearing area ( e.g. , trochlear cartilage ) and immediately transferred into a sterile transportation vessel containing calcium and magnesium free phosphate buffered saline solution at 4 c. biopsy specimens were transferred to royan institute , isfahan campus , for processing and culturing as previously described.1415 briefly , after enzymatic digestion of the tissue with 0.2% protease ( calbiochem , la jolla , ca ) for two hours in a 37c shaking water bath , chondrocytes were seeded into a medium cell culture flask containing whole cell culture medium ( including dmem / ham'sf12 ( dulbecco 's ) , modified eagle 's medium containing human serum ( 10% ) , ascorbic acid ( 25 g / ml ) , streptomycin ( 50 iu / ml ) and penicillin ( 50 iu / ml ) ) all obtained from seromed ( munich , germany ) and cultured until confluency was reached .
overall , chondrocytes were cultured for 2 - 3 weeks and alcian blue staining performed to confirm maintenance of chondrogenic phenotype before re - implantation .
for chondrocyte re - implantation , patients were readmitted to the hospital as previously described.1415 to prepare for chondrocyte implantation , a medial or lateral para - patellar arthrotomy either in left or right ( depended on the defect site ) was performed .
the defect was very deep , so an autogenous bone graft ( from proximal of tibia ) was placed in the depth of the defect before performing act . in the next step ,
the periosteal flap was harvested from the proximal medial tibia through a small separate incision over the anteromedial tibia just distal to the insertion of the pes tendons and was fitted and sutured to the surrounding rim of the cartilage with pds .
the periosteal flap was sealed to the rim with fibrin glue except for one upper corner , within which the cultured chondrocytes were later injected into the defect .
the flap was tested first with normal saline insufflations into the space between the periosteal flap and bone layers through a syringe - soft catheter setup to check that there was no leakage .
after chondrocyte injection beneath the periosteal flap , the remaining defect between the periosteal flap and rim was sutured with pds and sealed with fibrin glue ( berlin heart , gmibh , berlin , germany ) .
the postoperative rehabilitation program was a critical part of the treatment , including active and passive movement , muscle training , and weight - bearing exercises .
patients began physiotherapy with 030 angle of continuous passive motion beginning 6 hours after surgery .
the range of motion was gradually increased until 12 weeks , culminating in full flexion .
each patient remained non - weight - bearing for the 1 - 4 week , with partial weight - bearing exercise beginning after the 4 week . by the 12 week post act ,
sports activity was gradually increased after 6 months ; however , hard sporting activity was allowed only after 12 months .
the clinical status of patients was evaluated before , 6 and 12 months after act , using the brittberg - peterson functional assessment ( the best 0 to the worst 130 ) , and modified cincinnati rating score ( the worst 2 to the best 10 ) .
evaluation of mri findings was performed based on the scoring systems used by sally roberts and by henderson , by a blind skilled musculoskeletal radiologist .
wilcoxon statistical test was used for data analysis in spss ( version 18.0 ) and p - value < 0.05 was considered statistically significant .
this was a randomized clinical trial study that conducted in alzahra hospital in isfahan between 2004 - 2007 .
presented study consisted of 11 male patients with the mean age of 31.09 years ( ranged 20 to 44 years ) ; of which 90.9 % ( 10 patients ) had the chondral lesion in the medial condyle and 9.1 % ( one patient ) had the chondral lesion in lateral condyle of the femur .
patients presented themselves with isolated symptomatic cartilage defects , a limited chondral lesion ( mean surface area : 5 cm ) and without generalized osteoarthritis , genu valgum and/or genu varum of the knee .
patients under 40 years old with chief compliant of knee pain and up to three cartilaginous lesions between 2 - 9 cm were entered to study and patients lost to follow - up were excluded .
follow - up of all patients was for at least 12 months after surgery , additionally follow - up for 4 patients continued for 48 months .
the study was approved by the scientific & ethical committee of isfahan university of medical science ( iums ) , isfahan , iran ( no.186012 ) .
after decision for performing act was taken , approximately , a 200 - 300 mg full thickness cartilage biopsy from a healthy area of the joint was obtained from the outer edge of the superior medial condyle , from a non - weight - bearing area ( e.g. , trochlear cartilage ) and immediately transferred into a sterile transportation vessel containing calcium and magnesium free phosphate buffered saline solution at 4 c. biopsy specimens were transferred to royan institute , isfahan campus , for processing and culturing as previously described.1415 briefly , after enzymatic digestion of the tissue with 0.2% protease ( calbiochem , la jolla , ca ) for two hours in a 37c shaking water bath , chondrocytes were seeded into a medium cell culture flask containing whole cell culture medium ( including dmem / ham'sf12 ( dulbecco 's ) , modified eagle 's medium containing human serum ( 10% ) , ascorbic acid ( 25 g / ml ) , streptomycin ( 50 iu / ml ) and penicillin ( 50 iu / ml ) ) all obtained from seromed ( munich , germany ) and cultured until confluency was reached .
overall , chondrocytes were cultured for 2 - 3 weeks and alcian blue staining performed to confirm maintenance of chondrogenic phenotype before re - implantation .
for chondrocyte re - implantation , patients were readmitted to the hospital as previously described.1415 to prepare for chondrocyte implantation , a medial or lateral para - patellar arthrotomy either in left or right ( depended on the defect site ) was performed .
the defect was very deep , so an autogenous bone graft ( from proximal of tibia ) was placed in the depth of the defect before performing act . in the next step ,
the periosteal flap was harvested from the proximal medial tibia through a small separate incision over the anteromedial tibia just distal to the insertion of the pes tendons and was fitted and sutured to the surrounding rim of the cartilage with pds .
the periosteal flap was sealed to the rim with fibrin glue except for one upper corner , within which the cultured chondrocytes were later injected into the defect .
the flap was tested first with normal saline insufflations into the space between the periosteal flap and bone layers through a syringe - soft catheter setup to check that there was no leakage .
after chondrocyte injection beneath the periosteal flap , the remaining defect between the periosteal flap and rim was sutured with pds and sealed with fibrin glue ( berlin heart , gmibh , berlin , germany ) .
the postoperative rehabilitation program was a critical part of the treatment , including active and passive movement , muscle training , and weight - bearing exercises .
patients began physiotherapy with 030 angle of continuous passive motion beginning 6 hours after surgery .
the range of motion was gradually increased until 12 weeks , culminating in full flexion .
each patient remained non - weight - bearing for the 1 - 4 week , with partial weight - bearing exercise beginning after the 4 week . by the 12 week post act ,
sports activity was gradually increased after 6 months ; however , hard sporting activity was allowed only after 12 months .
the clinical status of patients was evaluated before , 6 and 12 months after act , using the brittberg - peterson functional assessment ( the best 0 to the worst 130 ) , and modified cincinnati rating score ( the worst 2 to the best 10 ) .
evaluation of mri findings was performed based on the scoring systems used by sally roberts and by henderson , by a blind skilled musculoskeletal radiologist .
wilcoxon statistical test was used for data analysis in spss ( version 18.0 ) and p - value < 0.05 was considered statistically significant .
this was a randomized clinical trial study that conducted in alzahra hospital in isfahan between 2004 - 2007 .
presented study consisted of 11 male patients with the mean age of 31.09 years ( ranged 20 to 44 years ) ; of which 90.9 % ( 10 patients ) had the chondral lesion in the medial condyle and 9.1 % ( one patient ) had the chondral lesion in lateral condyle of the femur .
patients presented themselves with isolated symptomatic cartilage defects , a limited chondral lesion ( mean surface area : 5 cm ) and without generalized osteoarthritis , genu valgum and/or genu varum of the knee .
patients under 40 years old with chief compliant of knee pain and up to three cartilaginous lesions between 2 - 9 cm were entered to study and patients lost to follow - up were excluded .
follow - up of all patients was for at least 12 months after surgery , additionally follow - up for 4 patients continued for 48 months .
the study was approved by the scientific & ethical committee of isfahan university of medical science ( iums ) , isfahan , iran ( no.186012 ) .
after decision for performing act was taken , approximately , a 200 - 300 mg full thickness cartilage biopsy from a healthy area of the joint was obtained from the outer edge of the superior medial condyle , from a non - weight - bearing area ( e.g. , trochlear cartilage ) and immediately transferred into a sterile transportation vessel containing calcium and magnesium free phosphate buffered saline solution at 4 c. biopsy specimens were transferred to royan institute , isfahan campus , for processing and culturing as previously described.1415 briefly , after enzymatic digestion of the tissue with 0.2% protease ( calbiochem , la jolla , ca ) for two hours in a 37c shaking water bath , chondrocytes were seeded into a medium cell culture flask containing whole cell culture medium ( including dmem / ham'sf12 ( dulbecco 's ) , modified eagle 's medium containing human serum ( 10% ) , ascorbic acid ( 25 g / ml ) , streptomycin ( 50 iu / ml ) and penicillin ( 50 iu / ml ) ) all obtained from seromed ( munich , germany ) and cultured until confluency was reached .
overall , chondrocytes were cultured for 2 - 3 weeks and alcian blue staining performed to confirm maintenance of chondrogenic phenotype before re - implantation .
for chondrocyte re - implantation , patients were readmitted to the hospital as previously described.1415 to prepare for chondrocyte implantation , a medial or lateral para - patellar arthrotomy either in left or right ( depended on the defect site ) was performed .
the defect was very deep , so an autogenous bone graft ( from proximal of tibia ) was placed in the depth of the defect before performing act . in the next step ,
the periosteal flap was harvested from the proximal medial tibia through a small separate incision over the anteromedial tibia just distal to the insertion of the pes tendons and was fitted and sutured to the surrounding rim of the cartilage with pds .
the periosteal flap was sealed to the rim with fibrin glue except for one upper corner , within which the cultured chondrocytes were later injected into the defect .
the flap was tested first with normal saline insufflations into the space between the periosteal flap and bone layers through a syringe - soft catheter setup to check that there was no leakage .
after chondrocyte injection beneath the periosteal flap , the remaining defect between the periosteal flap and rim was sutured with pds and sealed with fibrin glue ( berlin heart , gmibh , berlin , germany ) .
the postoperative rehabilitation program was a critical part of the treatment , including active and passive movement , muscle training , and weight - bearing exercises .
patients began physiotherapy with 030 angle of continuous passive motion beginning 6 hours after surgery .
the range of motion was gradually increased until 12 weeks , culminating in full flexion .
each patient remained non - weight - bearing for the 1 - 4 week , with partial weight - bearing exercise beginning after the 4 week . by the 12 week post act ,
sports activity was gradually increased after 6 months ; however , hard sporting activity was allowed only after 12 months .
the clinical status of patients was evaluated before , 6 and 12 months after act , using the brittberg - peterson functional assessment ( the best 0 to the worst 130 ) , and modified cincinnati rating score ( the worst 2 to the best 10 ) .
evaluation of mri findings was performed based on the scoring systems used by sally roberts and by henderson , by a blind skilled musculoskeletal radiologist .
wilcoxon statistical test was used for data analysis in spss ( version 18.0 ) and p - value < 0.05 was considered statistically significant .
the outcome of the act was rated to be good or excellent by 82% of the patients after 12 months . in the 4 patients who were followed for 48 months rating
the results of brittberg - peterson functional assessment for all 11 patients were a mean of 79.27  16.93 before act and declined to 25.82  7.33 and 19.27  6.05 at 6 and 12 months post act ( table 1 ) .
statistical analysis revealed a significant difference between brittberg - peterson functional assessment scores before act compared to 6 months ( p < 0.001 ) and 12 months ( p
there was also a statistical significant difference between 6 and 12 months post act in brittberg - peterson functional assessment ( p = 0.001 ) .
in the 4 patients that were followed for 48 month post act , brittberg - peterson functional assessment decreased from 86.25  17.02 before act to 11.75  2.36 48 months after act , and was statistical significant ( p = 0.003 , p = 0.001 and p = 0.001 respectively ) .
the results of wilcoxon test demonstrated a statistical difference ( p = 0.007 ) for the score before act to 6 , 12 and 48 month post act .
brittberg - peterson functional assessment before , 6 , 12 and 48 month after surgery the modified cincinnati rating score for all patients was 2.73 1.01 before act .
it improved to 7.27  1.35 and 8.36  1.50 at 6 and 12 months post act ( table 2 ) .
statistical analysis revealed a significant difference of modified cincinnati rating score comparing patients before and after 6 months ( p < 0.001 ) and 12 months ( p
a statistically significant difference of the modified cincinnati rating score was also found between 6 and month follow up post act ( p = 0.007 ) . in
the 4 patients which were followed up to 48 month post act , modified cincinnati rating score increased from 2.50  1.0 before act to 9.50  1 after 48 month post act , and was statistically significant ( p = 0.001 ) .
modified cincinnati rating score before , 6 , 12 and 48 month after surgery para clinical evaluation by the mri scoring system revealed a score of 2.5 for robert criteria ( table 3 ) and a score of 6.5 for henderson criteria ( table 4 ) .
the aim of the presented clinical study was to evaluate the clinical outcome of act on circumscribed human knee cartilage defects after follow up of 6 , 12 or 48 months .
excellent clinical outcome on all 11 patients after 6 , 12 and 48 months follow up was obtained as defined by modified cincinnati score , brittberg - peterson assessment and para - clinical mri scoring by henderson and robert criteria .
the outcome of the act 12 months after treatment was rated as good or excellent by 82% of the patients . in 4 patients who were followed for 48 month this rate was 100% . in the presented study we compared the modified cincinnati rating score and brittberg - peterson functional assessment in regard to their influence on act outcome rating .
the findings reveal that the cincinnati score increased after act treatment while the brittberg - peterson functional assessment score decreased after act , thus suggesting act improves functional capacity of knee including range of motion and decreases the symptoms of articular defects including pain while sitting , and/or during joint movement , locking , joint instability and running .
para clinical evaluation by mri scoring revealed a score of 6.5 out of 14 for henderson criteria and a score of 2.5 out of 4 for robert criteria .
the results of robert criteria were higher than in a previous study by moriya et al.,16 while our henderson score was lower than what had been reported by them , thus suggesting that our act outcome was better in some aspects of mri scoring .
our results concur with other studies that excellent results for graft integrity and short term follow up of act were observed.11718 in a systematic review on act by jobanputra et al . on 2600 patients
, it was concluded that the outcome of the act two years after treatment was rated as good or excellent by 70% of the patients.18 furthermore , erggelet and colleagues determined act as  a safe and effective method for the treatment of large full thickness cartilage defects.1 clinical assessment of act by bentley et al . also showed that 88% of patients had excellent or good results and 82% had excellent or good repairs in arthroscopy at one year post act.17 in a multicenter cohort study
, it was further concluded that  act is a viable treatment option that may yield relatively long - term symptomatic relief and functional improvement.19 in our study the modified cincinnati rating score improved from 2.73 before act to 7.27 , 8.36 and 9.5 at 6 , 12 and 48 month post act .
, were the modified cincinnati rating score improved from 2.2 before act to 7.6 , 9 , and 9.6 at 6 , 12 , and 24 months post act.20 however , although our results and results from the above mentioned studies were excellent , several studies also found contradictory findings after knee act .
odriscoll et al.11 did not recommend act in general population and gikas et al.21 even suggested that 80% of the patients have early pain relief but after 8 years 80% have osteoarthritis and 75% showed poor clinical results .
furthermore , clar et al.22 concluded that there is insufficient evidence to suggest that act is cost - effective compared with microfracture or mosaicplasty and horas et al.23 reported that the defects treated with act were primarily filled with fibrocartilage . a study by knutsen et al.24 further suggested that microfracture is a less costly and less invasive procedure and should be preferred as the first - line cartilage repair procedure and that act may be preferred only as a second - line treatment .
however , it is important to note that inclusion criteria of these studies were broad , and little consideration was paid to patients age or the size and location of the defects .
mandelbaum et al.12 also report that the degree of improvement was not significantly different between patients who had a concurrent procedure with act than those who did not , however they did not take into consideration the history of failed marrow stimulation procedure in their exclusion criteria and 48% of their case had a history of failed marrow stimulation .
the findings that were obtained in this study provided clear evidence that indeed the auto logous chondrocyte transplantation is an excellent method for full thickness cartilage repair of limited size and well - defined human articular knee cartilage defects as considered in this study .
kn has planned the study and finalized it . as collect the data and did the statistical analysis and prepared the first version of manuscript .
 background : although a variety of strategies have been employed for managing articular cartilage defects in the knee , overall outcomes have not been satisfactory 
 . an alternative option may be autologous chondrocyte transplantation ( act ) . 
 however , as this method is still under investigation , here we assessed the efficacy of act for human knee defect cartilage repair.methods:in a randomized clinical trial study , eleven patients ( mean age 31.09 years ) were enrolled in the study with full thickness cartilage defects in the knee . 
 arthroscopically , healthy cartilage was obtained , chondrocytes expanded for 2 - 3 weeks and act performed . 
 clinical status was evaluated before act , 6 and 12 months after act using the brittberg - peterson functional assessment and modified cincinnati rating score . magnetic resonance imaging ( mri ) 
 findings were evaluated based on the scoring systems used by sally roberts and by henderson.results:modified cincinnati rating indicated significant improvement of clinical score before act compared to 6 ( p = 0.000 ) and 12 ( p = 0.000 ) months after act ( from 2.73 before act to 7.27 , 8.36 and 9.5 at 6 , 12 , and 48 months after act , respectively ) . 
 brittberg - peterson functional assessment indicated a decline from 79.27 to 25.82 and 19.27 at 6 and 12 months post act . further 
 , statistical test demonstrated significant differences 6 , 12 and 48 months post act ( p = 0.007 ) . 
 evaluation of mri revealed a score of 6.5 for henderson criteria and a score of 2.5 for robert criteria.conclusions:our study demonstrated that act of the knee provides an excellent treatment for full thickness cartilage defects with outstanding clinical and radiological outcomes . 
Methods
None
Study design
Chondrocyte isolation and culture
ACT procedure
Post-operative rehabilitation
Evaluation of ACT outcome
Statistical analysis
Results
Discussion
Conclusion
Authors Contributions