Can Kakashi still use Susanoo after losing his Sharingan? When Kakashi had double Sharingan, he was able to use Susanoo. However, he lost the Sharingan.

Can he still use Susanoo without Sharingan now?
No, he can't now.

During the battle with Kaguya, Obito lent Kakashi the full power of the Mangekyo Sharingan which gave him the ability to use Susanoo. But after the battle, Kakashi lost his Sharingan along with Susanoo. So, I don't think that Kakashi can use Susanoo after all.