Can Sasuke use Tsukuyomi? The question is based on my theory from the recent manga chapter (Naruto 661):


  Sasuke, when stabbed by Madara is the effect of Tsukuyomi from Sasuke.


Now, to prove my theory, is there an episode in manga/anime that Sasuke used Tsukuyomi?
Short answer

Sasuke can not use Tsukuyomi. 


When fighting Kabuto, Sasuke used a regular Sharingan genjutsu on Itachi.
Sasuke's left eye creates Amaterasu, and his right eye manipulates the flames. There is no place for Tsukuyomi.


Long answer

Mostly from a reddit post:

Sasuke does not have the Tsukuyomi. What Sasuke uses is the Sharingan's standard ability to cast genjutsu through eye contact with its "eye of hypnosis". In the past, Sasuke had personally it used on Sai, Orochimaru and Deidara, and we've seen it used by Itachi, Kakashi, Obito and Madara on different occasions. What Sasuke had been doing since Itachi's death was channeling these Sharingan Genjutsu through his Mangekyou Sharingan, which apparently had the effect of amplifying its strength, rendering him capable of instantly incapacitating his opponents.

Sasuke was shown casting genjutsu with one eye of his Mangekyou, like how Itachi cast Tsukuyomi with his left eye (and Amaterasu with his right). But whilst Sasuke typically cast it with his right eye, he had also done it with his left eye. Against C too since he appeared to be holding his left eye, and a third time, if you count his domination of the bijuu.

And even regular Sharingan Genjutsu had been used through a single eye, as demonstrated by Madara and Itachi. Itachi himself used Sharingan Genjutsu with the Mangekyou after his Tsukuyomi eye had been blinded. Madara had done the same.

Sasuke has never once called his genjutsu, Tsukuyomi. Both Danzo and Tobi compared it to Itachi's Tsukuyomi - which was understandable since they're brothers and both had used Amaterasu and Susanoo - but neither of them actually called it Tsukuyomi. Only twice had Sasuke "named" it where he called it "Sharingan" and "Genjutsu - Sharingan" respectively (just like Madara). When he called it "Genjutsu - Sharingan", the difference is also made explicit by showing it side-by-side with Itachi's Tsukuyomi.

Tobi implied that Sasuke had received Itachi's eye techniques, but Itachi had only sealed a single use of the Amaterasu inside Sasuke with the Transcription Seal: Amaterasu which Sasuke had no actual control over. From what I've heard, it came from a misunderstanding over how the Japanese language doesn't differentiate between singular and plural. And when the jutsu was unsealed, his eye transformed into Itachi's Mangekyou Sharingan temporarily. So if Sasuke used a Tsukuyomi (or another Amaterasu) from Itachi, his eyes would have changed to show it.

The genjutsu Sasuke used against B (when he still had to implant Itachi's eyes) resembles the Tsukuyomi with its inverted colours, but this was also the only time it happened. Every other time we saw the genjutsu itself, it didn't have that visual effect. It was also said by Itachi and the second databook that the Tsukuyomi can only be broken by his own blood (presumably someone of the Uchiha bloodline with a powerful Sharingan), which we later saw proven and explained. Yet B was essentially freed with just a genjutsu dispel.

Ultimately, Sasuke only has Amaterasu, Kagutsuchi and Susanoo with his Mangekyou Sharingan. Amaterasu was first used with his left eye against Killer B. Kagutsuchi was used to quell the flames and then used and named it against the Raikage where he shaped the black flames and dubbed it "Enton: Kagutsuchi". The most blatant evidence of these two being his Mangekyou jutsu is when he fought Kaguya and freed himself from ice using both of his eyes.

Even after Sasuke took Itachi's eyes to awaken the Eternal Mangekyou Sharingan, he has never revealed the Tsukuyomi. You can implant the Mangekyou Sharingan and use the abilities of the original holder (e.g. Kakashi using Kamui or Danzo using Kotoamatsukami), but when those eyes are used to evolve to the next stage, it instead empowers their own abilities from what we've seen. Or at least removes the drawbacks.

Once the third databook stated that you would need Amaterasu and Tsukuyomi to awaken the Susanoo, but the above tells us that this is outdated information. If anything, I'd believe that Kishimoto had originally intended to give Sasuke the same jutsu as his brother (with different preferences, perhaps) but decided otherwise at crunch time. As far as we know, the only character with the Tsukuyomi is Uchiha Itachi.

Also, the current revision of Tsukuyomi from Naruto Wiki shows only Itachi Uchiha under the list of users.