Can Rikudou Madara activate his Limbo Perfect Susanoo in the anime after Mugen Tsukuyomi ends? Rikudou Madara in "Naruto Shippuden: Ultimate Ninja Storm 4" activates his Limbo Perfect Susanoo, and  uses it against Naruto and Sasuke.

Can he activate Limbo Perfect Susanoo in the anime? I think it is amazing  to  see final battle with Madara before his death.
He never does, but he theoretically could. He's still an Uchiha, and still has the Rinnegan and also uses Limbo while in Six Paths mode, however there is no reason for him to. The Truth Seeking Balls are a more perfect defense than Susano'o and the new abilities granted to him by the Six Paths' chakra seemed to be at least as powerful, if not moreso, than his Susano'o.