Histogram measurement in single spot I have a video that I shot for scientific purposes. I would like to measure the colour information in a single spot and export for example the RGB or CMYK values of that spot through every frame into a text file.

The video itself is a .MOV file which I colour-balanced with a white card. I just couldn't find any information on how to extract the information with any software. My university provides Adobe Photoshop in which I am able to open the video.

Does anyone know a software which enables me to extract that data? Or does someone know how I can do that with Photoshop?
The first thing I'd be looking at is Processing. There's a library that lets you read video, there's an example here that shows how to to sequentially grab frames from a video.

Then (instead of displaying the frame like it does in that example) you need to find the channel values for the spot which you could do by copying the frame to a PImage. Then the value of the pixel (x,y) is found in the PImage property pixels[x,y]. Finally use createWriter() to output the data to a file. You could even get fancy and use the mouse to control your sample point, and so on.