Since Mavericks, I can't mount ISO files on my Mac over a network Since upgrading to OS X Mavericks, when I want to mount an ISO file that's on my Mac from my Windows 8.1 PC (so, mounting a Mac share on a Windows computer, so I am accessing the file from the Windows PC), I get an error saying it can't mount it. I have to copy the file to the PC to mount the ISO file. 

Is this due to how Mavericks switched from AFP/SMB to SMB2? Is there a way to fix this?
It sounds more to me as if it's a Windows issue than a macOS issue, even though I realize that it didn't start until after the Mavericks upgrade.  Have you tried any other mount tools, such as CloneDrive or PowerISO?  It may be worth trying a couple of other tools as a test.  Are you able to mount the ISO from another Mac?