" Item can't be played " whenever I tap on a song I'm currently using iPhone 6 with iOS9.3.1. Whenever I try to play music I get an error. After I tap on a song I get the error item can't be played.  Also, I have tried restarting my iPhone multiple times but it doesn't seem to work.
This happened to me too (itunes 12.4.1.6-ipad 2). If you're trying to sync over wi-fi, try plugging in directly with the cable... worked for me.