How to test a sample of cigarette tobacco for its nicotine content? I was wondering how one would go about testing samples of different cigarette tobaccos for their nicotine content. Is there a kit of some kind available online to do this?
Unfortunately, nicotine analysis is prohibitively complicated and expensive for a home kit. The presence of other alkaloids similar to nicotine pretty much necessitates that somewhat expensive equipment (i.e. a gas chromatograph) is used to first separate out the nicotine prior to analysis (typically by mass spectroscopy).  

The only practical means for a layperson to test tobacco products for nicotine content is to out-source the work to a GC-MS equipped laboratory that routinely performs such analysis.