Purification of Solid Phase Synthesized Peptide I began working in a newly setup lab, and I'm having a dilysine (K2) peptide synthesized by solid phase method. I plan to cleave the peptides from the resin with a cleavage cocktail of TFA. Unfortunately, our freeze-drier is missing a part and not working then. We also don't have a rotary evaporator yet. We have our HPLC setup, but do you think it is a good idea to take the resulting solution of cleavage (after filtration to remove beads), which contains Boc, Fmoc, TFA, and possibly other impurities, directly to the HPLC?

I've read that HPLC sample should be dissolved in initial composition of my HPLC solvents, but it is in the case of having a crude dry sample.

It is my first time doing a peptide synthesis so I appreciate your ideas.
If you are a using reversed-phase (RP) column, it is okay to directly inject your crude sample in the running HPLC solvent system.  But, make sure to filter the injecting sample through a membrane filter to remove minute particles (from glass beats, etc.) from the original sample after dissolving it in suitable chosen solvent. Please also note that your chosen solvent for peptide separation usually contain small percentage of TFA. Therefore, you may need at least a rotary evaporator  for final concentration.