What should I do about a tensioned/trued wheel that lost significant spoke tension after first use? My front and rear wheels needed truing, so I picked up tools to do it.  Rims are DT Swiss R460: 20 spokes front, 24 back.  Max spoke tension is 1200N.
I got my front wheel to a left-side tension range of 685-889N and a right-side tension range of 685-938N, and I got my rear wheel to a left-side tension range of 540-720N and a right-side tension range of 758-991N.  Numbers are without tire or tube and after de-stressing both sides of each wheel.  I adjusted each spoke using tighten-then-back-off spoke wrench technique.  I didn't use spoke prep -- figuring it wasn't strictly necessary for an existing wheel if I was attentive and executed tighten-then-back-off correctly.
I put tires and tubes on the wheels and reinstalled them, then did 13mi Sunday.  The rear wheel felt maybe more &quot;taut&quot; than normal (from fresh reinflation to max 120psi?), but otherwise things went fine.
It's now Tuesday, and I figured it'd be a good idea to recheck tensions after a ride and some time.  Didn't remove the tires, just popped the bike in a stand and ran the TM-1 over a few spokes.  The tensions were much lower, 14-16 on a TM-1 versus 17-22 before for a few random left-side spokes on each wheel.  (Maybe &quot;taut&quot; is not actually what I was feeling in the rear wheel.)  That decrease isn't normal -- is it?
Should I just tighten all the spokes to get them to a more desirable tension range again?  Is it normal for tension to decrease so much from putting an inflated tire on a wheel?  (This answer suggests no.)  Should I remove (or at least deflate) the tires before doing so, and if so should I tension beyond 1200N assuming some loosening when a tire is added again?  (This answer again suggests no.)  Or is my no-spoke-prep tactic coming back to bite me, prior tensioning would likely have been fine if I'd used some, and I should apply some to every spoke and then tension things up?
Yes, it’s normal for spoke tension to decrease when the tire is inflated.
First of all I’d use much more tension and try to get it more even. Currently you have about 30% difference between slackest and tautest spoke, which is a lot (I’d aim for ~10%, but I’m a perfectionist in that regard).
540N on the slackest rear spoke before you’ve even inflated the tire is very little. I’d get the drive side spokes to slightly over the allowed 1200N and hope that the non-drive side ends up around 700N.
I’d also aim for 900N on the front wheel.
All tensions assuming zero tire pressure.
Considering the low spoke count and the high tire pressure you are using you could go for even higher (initial) spoke tension.
I’ve had good experience with using grease on the spoke nipples (on contact surfaces and thread). Makes truing much easier, avoids wear from truing and also avoids deforming the spokes or nipples from all the tension force.