I have the following simple code:

```
<asp:UpdatePanel runat="server">
    <ContentTemplate>
    <asp:LinkButton runat="server" Text="Refresh"></asp:LinkButton>
    </ContentTemplate>
</asp:UpdatePanel>

<asp:UpdatePanel ID="UpdatePanel1" runat="server">
    <ContentTemplate>
    <asp:Label ID="LinkButton1" runat="server" Text="<%# DateTime.Now.ToString() %>"></asp:Label>
    </ContentTemplate>
</asp:UpdatePanel>

```

And in the master page there is a ToolkitScriptManager. but the update panel does not work never similar to they do not exist.

Note: I use .Net 4.0.

**Edit:** I removed the ToolKitScriptManager and added ScriptManager and the problem still exists. Seems the Ajax functionality is disabled in my application but I do not know how.

Any help!