Preparation method: Human blood vessel: artery tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human blood vessel: artery tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated blood vessel: artery tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated blood vessel: artery tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
Applications: Blood Vessel: Artery cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.