CX3CR1 cells (green), macrophages (blue), B. anthracis spores (red) in the lungs. Confocal microscopy performed ex vivo. Click on the image to run the animation.
Alveolar collagen (SHG, grey), CX3CR1 cells (green), B. anthracis spores (red) in the lungs. Two-photon experiment performed ex vivo. Click on the image to run the animation.
How does the body cope with sepsis?
To help answering this very broad question, I develop new imaging tools based on two-photon microscopy of the living mouse. I especially investigate in vivo the altered behavior of microglia in the brain, immune cells in the lungs and lymph nodes, and satellite cells in the muscles.
Understanding the role of the microglial cells in the septic associated encephalopathy and the impact of molecular modulation.
After my PhD thesis (2010-2013) at the French Armed Forces Biomedical Research Institute, which dealt with the study of immune cells interaction in the lungs of infected mice using two-photon intravital microscopy, I worked as a postdoc at the University of California, Los Angeles (UCLA) on imaging the cortical plasticity after stroke at the cellular and functionnal level.
Fiole D, Tournier JN, Intravital microscopy of the lung: minimizing invasiveness, J Biophotonics 2016 Feb;.
Fiole D, Deman P, Trescos Y, Mayol JF, Mathieu J, Vial JC, Douady J, Tournier JN, Two-photon intravital imaging of lungs during anthrax infection reveals long-lasting macrophage-dendritic cell contacts, Infect. Immun. 2014 Feb;82(2):864-72.
Daniel Fiole, Thèse : Apports de la microscopie biphotonique intravitale pulmonaire à l’étude de la physiopathologie de la maladie du charbon., Daniel Fiole. Apports de la microscopie biphotonique intravitale pulmonaire à l'étude de la physiopathologie de la maladie du charbon. Université de Grenoble, 2013..
Fiole D, Douady J, Vial JC, Quesnel-Hellmann A, Tournier JN, Dynamics of rapid spore capture by dendritic cells in the lung alveolus, Am. J. Respir. Crit. Care Med. 2012 Jul;186(2):e2-3.
Fiole D, Touvrey C, Quesnel-Hellmann A, Douady J, Tournier JN, Shape-based tracking allows functional discrimination of two immune cell subsets expressing the same fluorescent tag in mouse lung explant, PLoS ONE 2012;7(6):e39831.
Klezovich-Bénard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL, Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins, PLoS Pathog. 2012 Jan;8(1):e1002481.
Garraud K, Cleret A, Mathieu J, Fiole D, Gauthier Y, Quesnel-Hellmann A, Tournier JN, Differential role of the interleukin-17 axis and neutrophils in resolution of inhalational anthrax, Infect. Immun. 2012 Jan;80(1):131-42.