IL-3 stands for interleukin 3. Human IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro , for example, CD34+ cells from umbilical cord blood2 .
In vitro differentiation studies, for example, of B lymphoid progenitors3 .
Cultivation of plasmacytoid dendritic cells4 .
In vitro expansion of hematopoietic stem cells.
Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5 .
Investigation of IL-3–mediated signaling pathways.
Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.
. The biological activity of Human IL-3, premium grade was determined by proliferation assay using TF-1 cells.
from Miltenyi Biotec (MB) in comparison to recombinant human IL-3 from a different supplier (S), both under reducing and non-reducing conditions.
Mass spectrometry analysis (ESI-MS) of Human IL-3, premium grade. The peak corresponds to the calculated molecular mass of 15089 Da.
expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of SCID repopulating cells.