Standard operating procedure to verify system suitability software results Vs manually of the high Performance liquid chromatography for result accuracy.
To Verify system suitability values manually v/s software.
This procedure is applicable to verify system suitability software results Vs manually of the high-performance liquid chromatography.
5.1 Frequency: Once in 6 months.
5.2 Operate the instrument as per respective standard operating procedure of HPLC.
5.3 Prepare the mobile phase consisting of 70% methanol. : 30 % of water.
5.4 Prepare a mixture of 0.1 % Benzene and 0.1% Toluene in methanol.
5.5 Set flow rate at 1.0 ml/min.
5.6 Allow the system to be saturated with mobile phase for at least 15 minutes before injecting the test sample.
5.7 Record the area and retention time of both Benzene and Toluene in methanol.
5.8 Calculate the system suitability parameters with the help of software such as resolution, tailing factor and theoretical plate.
The performance of software of the instrument is satisfactory if the all the parameters of the system suitability test are within the acceptance criteria.