Discrete multi-tone (DMT) technology is an attractive modulation technique for short reach optical transmission system. One of the main factors that limit system performance is fiber dispersion, which is strongly influenced by the chirp characteristics of transmitters. We investigated the fiber dispersion impairment in a 400GbE (4 × 116.1-Gb/s) DMT system on LAN-WDM grid for reach enhancement up to 40 km through experiments and numerical simulations.