Rinse in several changes of Alcohol, 80% pH 1.0 (#26328-03).
Stain in Aldehyde Fuchsin Solution (#26328-01) for 30 minutes.
Rinse off excess stain with Alcohol, 80% pH 1.0 (#26328-03).
Counterstain with Metanil Yellow Solution (#26328-02) until background is a light yellow (approximately 1 minute). Rinse in distilled water.
Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene (#23400), two changes each.
All mucins except the highly acidic sulfated mucosubstances are aldehyde fuchsin negative at pH values of 1.0 and below.
Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964.
Aldehyde Fuchsin (pH 1.0) Method is available online from the EMS Catalog. For ordering or product information, click here.