Among OSB populations, genetic diversity is lowest at Mount Hebo and highest at Rock Creek and Bray Point. Of the 32 haplotypes detected in OSB, only 2 were shared among populations (1 shared by Mount Hebo, Cascade Head, Bray Point, and Rock Creek, and 1 shared by Rock Creek and Lake Earl). The remaining 30 haplotypes were identified in individual populations, highlighting the strong differentiation among sites. It is unclear if the shared haplotypes represent widespread, naturally occurring genetic variation or if allele sharing among populations is due to translocation history.
Using full siblings of individuals that were released at Rock Creek and Bray Point in 2012 as comparison standards, the analyses suggest that 54 percent of the sampled individuals from Bray Point were naturally recruited into the population and were not originating from the 2012 release of captive reared individuals. Likewise, 33 percent of the analyzed individuals from Rock Creek were naturally recruited. Both of these estimates may be underestimates if the shared alleles that we identified among populations are naturally occurring and not a product of the 2012 translocations.
The results suggest that there are about 12–13 COI haplotypes in the Mount Hebo population. The U.S. Fish and Wildlife Service anticipates using Mount Hebo as the source of individuals when establishing new populations in the future. Nonlinear regression models based on a series of rarefaction analyses suggest that progeny from 12, 37, 109, and 326 female individuals would be required to respectively capture 25, 50, 75, and 90 percent of the allelic diversity from Mount Hebo.
Phylogenetic analyses identified two different haplotype groups, but the two groups did not correspond to the different subspecies used in the analysis. One group included 22 S. z. hippolyta haplotypes and 7 haplotypes identified in S. z. gloriosa. The second group included eight haplotypes from S. z. hippolyta, three haplotypes from S. z. gloriosa, and one haplotype that was detected in both subspecies.