dc.identifier.citation Kogan M, Beke-Somfai T, Nordén B (2011) Flow-alignment of bicellar lipid mixtures: orientations of probe molecules and membrane-associated biomacromolecules in lipid membranes studied with polarized light. Chem Commun 47: 7356. Available: http://dx.doi.org/10.1039/c1cc12313k.
dc.description.abstract Bicelles are excellent membrane-mimicking hosts for a dynamic and structural study of solutes with NMR, but the magnetic fields required for their alignment are hard to apply to optical conditions. Here we demonstrate that bicellar mixtures can be aligned by shear forces in a Couette flow cell, to provide orientation of membrane-bound retinoic acid, pyrene and cytochrome c (cyt c) protein, conveniently studied with linear dichroism spectroscopy. © 2011 The Royal Society of Chemistry.
dc.description.sponsorship This work is financed by King Abdullah University of Science and Technology (KAUST) (Grant KUK-11-008-23) and the European Research Council (ERC-2008 AdG 227700-SUMO).
High anisotropy of flow-aligned bicellar membrane systems.
Bicelles: A natural 'molecular goniometer' for structural, dynamical and topological studies of molecules in membranes.
Magnetically aligned bicelles to study the orientation of lipophilic ligands in membrane bilayers.
Not only oxidation of cardiolipin affects the affinity of cytochrome C for lipid bilayers.