Diterpenoids salvimulticanol (1) and salvimulticaoic acid (2) together with known diterpenoid (3-6) were isolated from Salvia multicaulis. Structures were elucidated by spectroscopic techniques including HRESIMS as well as 1D-, and 2D-NMR. In-vitro cytotoxicity was assayed against human cancer cell lines. As several metabolites exhibited activity against drug-resistance lines, compounds were screened against a panel of human drug-sensitive and multidrug-resistant cancer lines. A proposed biosynthetic pathway for these new diterpenoids (1-2) as well as the cytotoxic structure-activity relationship of all identified compounds were discussed. Compound 1 and 6 showed the most potent cytotoxicity with IC50 11.58 and 4.13 towards leukemia cell lines CCRF-CEM and CEM-ADR5000, respectively.
Hegazy MF, Hamed AR, El-Halawany AM, Hussien TA, Abdelfatah S, Ohta S, Paré PW, Abdel-Sattar E, Efferth T. Cytotoxicity of abietane diterpenoids from Salvia multicaulis towards multidrug-resistant cancer cells. Fitoterapia. 2018 Oct;130:54-60. doi: 10.1016/j.fitote.2018.08.002.
Keywords: cytotoxicity, abietane diterpenoids, Salvia multicaulis, multidrug-resistant cancer cells, CCRF-CEM, CEM-ADR5000, HRESIMS, 1D-, and 2D-NMR, salvimulticaoic acid, salvimulticanol.