Dornase alfa
Dornase alfa (proprietary name Pulmozyme from Genentech) is a highly purified solution of recombinant human deoxyribonuclease I (rhDNase), an enzyme which selectively cleaves DNA.   Dornase alfa hydrolyzes the DNA present in sputum/mucus of cystic fibrosis patients and reduces viscosity in the lungs, promoting improved clearance of secretions.  This protein therapeutic agent is produced in Chinese hamster ovary cells.

Cystic fibrosis
Dornase alfa is the most recent therapeutic agent developed with this basic mechanism of action.  Prior to the cloning of the human enzyme, bovine DNase I was on the market for many years, though its utility was limited by the inherent antigenic response to a cow protein in the lungs of patients.  Other DNases, such as DNase II, have therapeutic potential as well, but no further DNases have been brought to market yet for cystic fibrosis.
Dornase alfa is an orphan drug.

Contraindications
Hypersensitivity to dornase alfa
Hypersensitivity to Chinese hamster ovary cell products

Economy
Dornase alpha 1000 units (1 mg/ml)2.5ml (2500units) = £18.52 (GBP)

Off label use
Dornase alfa has recently been shown to improve lung function in non-cystic fibrosis pre-term infants atelectasis where other therapies have failed.In studies conducted, Dornase alfa has been thought to help resolve mucus secretion in COVID-19 patients as well as in the treatment of cystic fibrosis, but since it is taken with nebulizer in general use and there is a possibility of aerosolization of the virus, it is not preferred in most medical centers to avoid secondary infections.


== References ==