In addition to the pyruvate, the net products are two molecules glucose two molecules of ATP and two molecules of NADH. of pyruvate Step 2 An isomerization COO–
After the enzyme removes a proton from the CH3 group on acetyl CoA, the negatively charged CH2 – forms a bond to a carbonyl carbon of oxaloacetate. The subsequent loss by hydrolysis of the coenzyme A (HS–CoA) drives the reaction strongly forward. acetyl CoA S-citryl-CoA intermediate citrateoxaloacetate COO–COO–OOCCSCoAcitrate synthase CH3CH2H2OOCSCoACH2COO–COO–CHOCH2CH2COO–COO–COO–CCH2CoAHSH+CoAHO+++Details of these eight steps are shown below. In this part of the panel, for each step, the part of the molecule that undergoes a change is shadowed in blue, and the name of the enzyme that catalyzes the reaction is in a yellow box. CH2COO–COO–COO–CHOCH2H2OH2OH2OCH2CO2CO2CO2COO–COO–COO–HCCHHOCH2COO–COO–COCH2CH2COO–COO–CH2CHCOO–COO–CHHOHCCOO–COO–CH2COO–COCH2SCoAOCH3SCoAacetyl CoA coenzyme A HSCoAHSHSCoAHSCoACH2OCCOO–COO–CH2OOCCOO–COO–COO–CH2CH3Cnext cycle + Step 1 Step 2 Step 3 Step 4 Step 6 Step 7 Step 8 Step 5 citrate (6C) isocitrate (6C) succinyl CoA (4C)succinate (4C) fumarate (4C) malate (4C) oxaloacetate (4C) oxaloacetate (4C) pyruvate ˜-ketoglutarate (5C) + H+ + H+ + H+ + H+ (2C) CITRIC ACID CYCLE Overview of the complete citric acid cycle. The two carbons from acetyl CoA that enter this turn of the cycle (shadowed in red ) will be converted to CO2 in subsequent turns of the cycle: it is the two carbons shadowed in blue that are converted to CO2 in this cycle. CGTPGDPNADHNADHNADHNAD+NAD+NAD+Pi FADFADH2Step 1 106 PaNel 2–9: The Complete Citric acid Cycle NADHNAD+ reaction, in which water is aconitaseadded back, moves the hydroxyl group from one carbon atom to its neighbor.