Since the newly formed synthesised DNA strand is forming from 5' to 3', does that mean that the DNA polymerase is reading the template DNA strand from 3' to 5'?
https://answers.yahoo.com/question/index?qid=20101113015720AAu0OKk
A nucleotide can be added to DNA polymerase only on a pre-existing 3’-OH group which requires a primer so that it may add to the nucleotide. The so called primers do contain DNA and RNA base. DNA has the base thymine while RNA has uracil as its base.
http://www.differencebetween.net/science/difference-between-dna-polymerase-and-rna-polymerase/
RNA is synthesized by RNA polymerase that reads one strand of DNA. RNA polymerase reads DNA 3' to 5'. When RNA is made, it is made 5' to 3'. Most polymerases have the 3' to 5' reading activity. The created RNA strand is identical to the coding strand of DNA, which is also in the orientation of 5' to 3'.
http://www.answers.com/Q/In_which_direction_does_RNA_polymerase_read_a_DNA_strand
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https://answers.yahoo.com/question/index?qid=20101113015720AAu0OKk
The problem with this model is that ribonucleotide-3'-triphosphates are less stable under acidic conditions due to the neighbouring 2'-OH (though this obviously only applies for RNA, not for DNA). So any 3'-5' polymerase would likely need to use the same nucleotide-5'-triphosphates as the 5'-3' polymerase.
http://biology.stackexchange.com/questions/477/why-is-dna-replication-performed-in-the-5-to-3-direction
1 Transcribing DNA to MRNA Transcription refers to the synthesis of RNA (mRNA or tRNA) from a one strand of DNA. 2  Messenger RNA (mRNA) carries the genetic information from DNA to the ribosomes to synthe…. 3  Transcription Termination Transcription termination is the last step in gene expression.
http://www.answers.com/Q/In_which_direction_does_RNA_polymerase_read_a_DNA_strand
DNA replication goes in the 5' to 3' direction because DNA polymerase acts on the 3'-OH of the existing strand for adding free nucleotides.
http://biology.stackexchange.com/questions/477/why-is-dna-replication-performed-in-the-5-to-3-direction
RNA is usually single stranded while DNA is double stranded. RNA uses the base uracil instead of the base thymine used by DNA. RNA polymerase does not need to use the enzyme h … elicase to unwind the strands of DNA, nor does it need a primer to transcribe. DNA polymerase needs both to replicate DNA.
http://www.answers.com/Q/In_which_direction_does_RNA_polymerase_read_a_DNA_strand
This is no significant problem for the 5'-3' polymerase, as the triphosphate is on the new nucleotide and the polymerase just has to find a new nucleotide. For the 3'-5' polymerase spontaneous hydrolysis is a problem because the triphosphate is on the growing chain.
http://biology.stackexchange.com/questions/477/why-is-dna-replication-performed-in-the-5-to-3-direction
If so, can I conclude that all polymerase, including RNA polymerase and DNA polymerase, read the template DNA strand from 3' to...
https://answers.yahoo.com/question/index?qid=20101113015720AAu0OKk
what direction does rna polymerase read dna
The RNA polymerase reads DNA in the 3' to 5' direction.