Save. In paper chromatography, RF stands for retention factor, or the distance a liquid compound travels up a chromatography plate. The chromatography paper is the stationary phase and the liquid compound is the mobile phase; the liquid carries the sample solutions along the paper.ith your ruler, measure the distance the solvent traveled, which is Df, and measure the distance the test solution traveled, which is Ds. Calculate the retention factor using this equation: RF = Ds/Df.
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With your ruler, measure the distance the solvent traveled, which is Df, and measure the distance the test solution traveled, which is Ds. Calculate the retention factor using this equation: RF = Ds/Df.Simply divide the distance the solution traveled by the distance the solvent traveled.The retention factor will always be between zero and one.ith your ruler, measure the distance the solvent traveled, which is Df, and measure the distance the test solution traveled, which is Ds. Calculate the retention factor using this equation: RF = Ds/Df.
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Examine the results of the chromatography performed in step 2. Measure the distance that the test substance traveled before dropping out of the solution (Ds) and the distance that the solvent traveled(Df). The retardation factor, Rf, is then given by Ds/Df. Interpret the retardation factor.The retardation factor is only meaningful if the test substance moves some measurable distance, and it can never move farther than the solvent.ach substance in the mixture does this at a specific rate, causing it to become separated as it moves into the stationary phase. The retardation factor (RF) is the relative amount of time that a particular substance spends in the mobile phase.
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Thus, Rf = Ds/Df ,where Rf is the retardation factor, Ds is the migration distance of the substance, and Df is the migration distance of the solvent front. Dip a strip of filter paper into a solution that contains a mixture of substances to be analyzed and a solvent.ach substance in the mixture does this at a specific rate, causing it to become separated as it moves into the stationary phase. The retardation factor (RF) is the relative amount of time that a particular substance spends in the mobile phase.
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Rf values are the ratio between the distance traveled by t … he spot of the analyte to the distance traveled by the solvent used.This is used in paper chromatography and thin layer chromatography.f=Distance travelled by pigment/ Distance travelled by the solvent.Rf is the distance travelled by a sample or analyte divided by distance travelled by the solvent front in c … hromatography.
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Best Answer: I assume you are doing chromatography. For paper chromatography the rf value is the ratio the spot moved compared to the distance the solvent moved from the starting point.Rf=distance spot moved/distance solvent moved.Source(s): drcjs_007 · 9 years ago.est Answer: I assume you are doing chromatography. For paper chromatography the rf value is the ratio the spot moved compared to the distance the solvent moved from the starting point.
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Chromatography is a collection of laboratory techniques for separating a mixture of substances. These techniques involve passing the mixture from a mobile phase to a stationary phase. Each substance in the mixture does this at a specific rate, causing it to become separated as it moves into the stationary phase.The retardation factor (RF) is the relative amount of time that a particular substance spends in the mobile phase.ach substance in the mixture does this at a specific rate, causing it to become separated as it moves into the stationary phase. The retardation factor (RF) is the relative amount of time that a particular substance spends in the mobile phase.
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Retention Value, is also known as Rf value. Retention Value, Rf can be defined as the ratio of distance moved by the solute and the distance moved by the solvent.f=Distance travelled by pigment/ Distance travelled by the solvent.Rf is the distance travelled by a sample or analyte divided by distance travelled by the solvent front in c … hromatography.
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Rf values do not have units since it is a ration of distances. 1  Rf = 0.66 (60% Ethanol) - if % is given it is assumed that the mixture is in water hence 60% ethanol 40% water. 2  Rf = 0.78 (Ethanol-Methanol mixture {1:2}) - a mixture of 1 part Ethanol and 2 parts Methanol.ue the fact that the solvent front is always larger from the distance travelled by the solute, Rf values are always between 0 - one extreme where solute remains fixed at its origin and 1 - the other extreme where the solute is so soluble that it moves as far as the solvent.
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Rf=Distance travelled by pigment/ Distance travelled by the solvent.Rf is the distance travelled by a sample or analyte divided by distance travelled by the solvent front in c … hromatography.+ 18 others found this useful.f=Distance travelled by pigment/ Distance travelled by the solvent.Rf is the distance travelled by a sample or analyte divided by distance travelled by the solvent front in c … hromatography.
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formula to calculate Rf
The formula to calculate Rf  is, Rf is equal to Ds divided by Df.