The polymerase chain reaction (PCR) is a biochemical technology in molecular biology used to amplify a single, or a few copies, of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Applications.
https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/bioinformatics-83/amplifying-dna-the-polymerase-chain-reaction-458-5372/
PCR works by repeated cycles of strand separation, annealing of primers, and extension of the primed strands. Simply put, the target DNA is replicated in vitro, many, many times, to obtain a large amount of the DNA that lies between the two primer regions.
http://oregonstate.edu/instruct/bb494/pcr.html
PCR stands for Polymerase Chain Reaction, a method for amplifying DNA in vitro. Where, in cloning DNA into a plasmid, the DNA is amplified by the bacterial cell when it replicates the plasmid, PCR amplifies DNA in a test-tube (when we speak of amplification in this context, we mean that many copies of a given DNA are being made).
http://oregonstate.edu/instruct/bb494/pcr.html
is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the first nucleotide.
https://www.ncbi.nlm.nih.gov/probe/docs/techpcr/
PCR is used to amplify a specific region of a DNA strand (the DNA target). Most PCR methods typically amplify DNA fragments of up to ~10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.
https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/bioinformatics-83/amplifying-dna-the-polymerase-chain-reaction-458-5372/
Since PCR is, basically, replication of DNA in a test-tube, all the usual ingredients needed for DNA replication are required: A template. Primers. DNA polymerase. dNTPs (deoxynucleoside triphosphates used to make DNA). The template is the DNA that contains the target sequence you want to amplify.
http://oregonstate.edu/instruct/bb494/pcr.html
In a PCR experiment, two primers are designed to match to the segment of DNA you want to copy. Through complementary base pairing, one primer attaches to the top strand at one end of your segment of interest, and the other primer attaches to the bottom strand at the other end.
http://learn.genetics.utah.edu/content/labs/pcr/
PCR (short for Polymerase Chain Reaction) is a relatively simple and inexpensive tool that you can use to focus in on a segment of DNA and copy it billions of times over. PCR is used every day to diagnose diseases, identify bacteria and viruses, match criminals to crime scenes, and in many other ways.
http://learn.genetics.utah.edu/content/labs/pcr/
What is PCR (polymerase chain reaction)? PCR (polymerase chain reaction) is a method to analyze a short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA. Previously, amplification of DNA involved cloning the segments of interest into vectors for expression in bacteria, and took weeks. But now, with PCR done in test tubes, it takes only a few hours.
http://www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm
PCR: How We Copy DNA. Polymerase chain reaction (PCR) is an efficient and cost-effective way to copy or “amplify” small segments of DNA or RNA. Using PCR, millions of copies of a section of DNA are made in just a few hours, yielding enough DNA required for analysis. This innovative yet simple method allows clinicians to diagnose and monitor diseases using a minimal amount of sample, such as blood or tissue.
https://molecular.roche.com/innovation/pcr/what-is-pcr/
what does pcr amplify
The polymerase chain reaction is a biochemical technology in molecular biology used to amplify a single, or a few copies, of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. The Polymerase chain reaction is a biochemical technology in molecular biology used to amplify a single and a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.