Working princple of flurosence microscope
Many substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Such substances are called ‘fluorescent substances’. Application of this phenomenon is the basis of fluorescence microscope. Working Principle: Many substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Such substances are called ‘fluorescent substances’. Application of this phenomenon is the basis of fluorescence microscope. The fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image. Such substances are called ‘fluorescent substances’. Application of this phenomenon is the basis of fluorescence microscope. In practice, microbes are stained with a fluorescent dye and then illuminated with blue light. 1 The conventional microscope uses visible light (400-700 nanometers) to illuminate and produce a magnified image of a sample. 2  A fluorescence microscope, on the other hand, uses a much higher intensity light source which excites a fluorescent species in a sample of interest. Many substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Such substances are called ‘fluorescent substances’. 1 A fluorescence microscope, on the other hand, uses a much higher intensity light source which excites a fluorescent species in a sample of interest. 2  This fluorescent species in turn emits a lower energy light of a longer wavelength that produces the magnified image instead of the original light source. The fluorescence microscope is based on the phenomenon that certain material emits energy detectable as visible light when irradiated with the light of a specific wavelength. The sample can either be fluorescing in its natural form like chlorophyll and some minerals, or treated with fluorescing chemicals. Working Principle: Many substances absorb light. However some of them, after absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy. Such substances are called ‘fluorescent substances’. In the life sciences fluorescence microscopy is a powerful tool which allows the specific and sensitive staining of a specimen in order to detect the distribution of proteins or other molecules of interest.
absorbing light of a particular wavelength and energy, emit light of a longer wavelength and lesser energy