anneal definition biology
Anneal. Anneal-Definition of Anneal at Dictionary.com a free online dictionary with ... anneal. O.E. onaelan onælan to set on, fire, kindle from- on + on aelan ælan, ... to burn Double-Check Published Tm Data. It is important to check the T m of any oligonucleotide sequences used in PCR even when a previously successful primer and probe set is taken directly from a publication. The design of such published sequences may incorporate T m enhancers such as a minor grove binder or LNA bases. 5. In molecular biology, annealing is a process in which short sections of single-stranded DNA from one source are bound to a filter and incubated with single-stranded, radioactively conjugated DNA from a second source. Where the two sets of DNA possess complementary sequences of nucleotides, bonding occurs. anneal. 1. to toughen, temper, or soften a material, as a metal, by controlled heating and cooling. 2. in molecular biology, to cause the association or reassociation of single-stranded nucleic acids so that double-stranded molecules are formed, often by heating and cooling. anneal. 1. to heat a material, such as glass or metal, followed by controlled cooling to remove internal stresses and induce a desired degree of toughness, temper, or softness of the material. For more information on the calculations and algorithm used by the OligoAnalyzer tool, refer to the IDT technical report, Calculation of T m for Oligonucleotide Duplexes [2]. References. 1  Owczarzy R, Moreira BG, et al. ( 2 2008) Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations. Full article ... Anneal. The sustained heating of a material such as steel or glass at aspecific high temperature, followed by gradual cooling, this is done to eliminate weakness or to produce other qualities. Anneal. Binding of a single-strand ed DNA to its complement ary single-strand ed DNA. The Importance of T m in Molecular Biology Applications. The melting temperature of an oligonucleotide duplex, or T m, is the temperature at which half of the oligonucleotide molecules are single-stranded and half are double-stranded, i.e., the oligonucleotide is 50% annealed to its exact complement. Related ... anneal. The joining of single strand s of DNA because of the pairing of complement ary bases. In PCR, primer s anneal to complement ary target DNA sequence s during cooling of the DNA (after DNA is made single strand ed by heating). Used in the context of dna renaturation after temperature dissociation of the two strands. Rate of annealing is a function of complementarity. 3. Fusion of microtubules or microfilaments end to end. Please contribute to this project, if you have more information about this term feel free to edit this page. This page was last modified on 3 October 2005, at 21:16.This page has been accessed 5,864 times. What links here | Related changes | Permanent link.
Binding of a single-strand ed DNA to its complement ary single-strand ed DNA.