Fine structure and primary sensory projections of sensilla located in the sacculus of the antenna of Drosophila melanogaster.
Sensilla lining the inner walls of the sacculus on the third antennal segment of Drosophila melanogaster were studied by light and transmission electron microscopy. The sacculus consists of three chambers: I, II and III. Inside each chamber morphologically distinct groups of sensilla having inflexible sockets were observed. Chamber I contains no-pore sensilla basiconica (np-SB). The lumen of all np-SB are innervated by two neurons, both resembling hygroreceptors. However, a few np-SB contain one additional neuron, presumed to be thermoreceptive. Chamber II houses no-pore sensilla coeloconica (np-SC). All np-SC are innervated by three neurons. The outer dendritic segments of two of these neurons fit tightly to the wall of the lumen and resemble hygroreceptor neurons. A third, more electron-dense sensory neuron, terminates at the base of the sensillum and resembles a thermoreceptor cell. Chamber III of the sacculus is divided into ventral and dorsal compartments, each housing morphologically distinct grooved sensilla (GS). The ventral compartment contains thick GS1, and the dorsal compartment has slender sensilla GS2. Ultrastructurally, both GS1 and GS2 are double-walled sensilla with a longitudinal slit-channel system and are innervated by two neurons. The dendritic outer segment of one of the two neurons innervates the lumen of the GS and branches. On morphological criteria, we infer this neuron to be olfactory. The other sensory neuron is probably thermoreceptive. Thus, the sacculus in Drosophila has sensilla that are predominantly involved in hygroreception, thermoreception, and olfaction. We have traced the sensory projections of the neurons innervating the sacculus sensilla of chamber III using cobaltous lysine or ethanolic cobalt (II) chloride. The fibres project to the antennal lobes, and at least four glomeruli (VM3, DA3 and DL2-3) are projection areas of sensory neurons from these sensilla. Glomerulus DL2 is a common target for the afferent fibres of the surface sensilla coeloconica and GS, whereas the VM3, DA3 and DL3 glomeruli receive sensory fibres only from the GS.