**Background:** Human-animal interfaces pose opportunities for pathogen spillover from animal hosts into humans through contact with animal blood, secretions, or other means. Reducing spillover is a concern in Egypt, where diseases like brucellosis and Middle East Respiratory Syndrome have potential to spread from animal to human. Identification of behavioral risk intervention points at human-animal interfaces in Egypt may inform interventions to reduce this risk.

**Methods & Materials:** Cross-sectional surveys were conducted at 5 abattoirs, 2 animal markets, and 1 camel farm in Egypt from October 2016 to May 2017. Participants completed a standardized questionnaire. Beliefs about animal to human disease risks and behavioral practices were assessed to generate prevalence ratios (PRs) and 95% confidence intervals (CIs) modeling unsafe behavioral practices using belief about animal to human disease risks as an independent variable. PRs were adjusted for potential confounders (age, gender, education, and site type).

**Results:** Overall, 700 individuals completed the questionnaire (75% from abattoirs, 22% from markets, and 3% from the farm). Of them, 492 (70%) did not believe there are risks associated with slaughtering or butchering animals while having an open wound, and only 194 (28%) were worried about diseases in live animals. Those who believe there are risks associated were significantly less likely to have not washed, rinsed, or bandaged their most recent wound from animal bites or scratches or from butchering or slaughtering animals (adjusted PR = 0.69, 95% CI = 0.52-0.91). Similarly, those who believe there are risks associated were significantly less likely to not use personal protective equipment (PPE) at work (adjusted PR = 0.75, 95% CI = 0.66-0.86). Among 378 abattoir workers using PPE, only 7 (2%) use a mask and 16 (4%) use gloves.

**Conclusion:** A belief that animals do not pose disease risks to humans was prevalent, highlighting a knowledge gap that could be addressed through educational intervention. A sizable portion of those interviewed engage in behavioral practices that may increase their risk of a zoonosis, such as not washing wounds from animals. A multi-faceted intervention that increases knowledge about zoonotic disease risk and provides material resources like PPE and training may reduce spillover risk at human-animal interfaces in Egypt.
