An investigation of human sperm pronuclear chromosome "gaps" using scanning electron microscopy.
A common cytogenetic finding in both Q-banded and solid Giemsa-stained preparations of pronuclear chromosomes obtained from cross-species fertilization of hamster oocytes by human sperm is the presence of a variable-length "gap" in the centromeric region. Scanning electron microscopy was used to investigate these altered chromosomal regions. The centromere in most eukaryotic organisms appears as a constricted region approximately 200-300 nm in diameter. In contrast, the gap portion of the centromeric region of pronuclear chromosomes was found to contain a chromatin fiber with a diameter of 80-150 nm. The detection of this fiber confirms that the chromosome arms are continuous, and the size of the fiber explains the gap appearance in the light photomicrographs. The morphology of the fiber is consistent with the concept that the normal chromatin packaging has been altered in varied regions within the centromere of these chromosomes.