Introduction {#S0001}
============

Lymphatic filariasis (LF) is one of the neglected tropical diseases (NTD) caused by three species of filarial worm: *Wuchereria bancrofti, Brugia malayi,* and *Brugia timori*. As of 1997, the disease was prevalent in 72 tropical and sub-tropical countries of the world.[@CIT0001] In 1997, the World Health Assembly[@CIT0002] declared elimination of LF as a public health problem. Subsequently, the World Health Organization (WHO) launched the Global Programme to Eliminate Lymphatic Filariasis (GPELF) in 2000 to achieve elimination by 2020.[@CIT0003] The elimination program has two components 1) interruption of transmission; and 2) morbidity management and disability prevention. For interruption of transmission, the strategy is annual single dose mass drug administration (MDA) of albendazole in combination with diethylcarbamazine (DEC) or ivermectin (IVM) to the LF endemic population. The program encompasses the following sequence of steps:[@CIT0004] 1) map LF endemic areas (\>1% of antigenemia (Ag), among 50--100 individuals (\>15 years), from two communities of an endemic district);[@CIT0005] 2) deliver MDA for a minimum of 5 years with an effective coverage of 65%; 3) conduct a transmission-assessment survey (TAS);[@CIT0004] 4) conduct post-MDA surveillance; 5) develop a dossier that documents the achievement of elimination targets; and 6) independent validation of the claim that elimination criteria have been achieved.

Mapping the areas endemic for LF is the pre-requisite for the countries to plan and implement the elimination program. The next step is to implement MDA in endemic areas for a minimum of 5 years with an effective coverage of 65%. The program is to be monitored every 6 months post-MDA by assessing filarial infection (microfilaria \[Mf\]/Ag) in sentinel and spot check sites. If the prevalence of Mf in all the sentinel and in the randomly selected spot check sites are \<1%, TAS is conducted to decide on stopping or continuing MDA. The TAS's decision to stop or continue MDA is based on a pre-determined number of Ag positives below which transmission is expected to be interrupted and hence MDA can be stopped, if not MDA is continued for another two rounds. TAS is repeated twice at 2--3 year intervals (post-MDA surveillance) to assess if transmission interruption is sustained after cessation of MDA following TAS 1. After successfully passing all the three TASs, a dossier is submitted to the WHO, which validates the claim of elimination made by the country and once validated, WHO acknowledges LF elimination in that country. As of 2017, 51 of the 72 LF endemic countries have fully implemented MDA, and WHO acknowledged after post-MDA validation in 11 countries, that LF is no longer a public health problem.[@CIT0006]

As GPELF is nearing its end game in many endemic countries it would be useful to do a comprehensive assessment of the mapping and monitoring activities used so far and to see how they been improvised to help in upscaling and assessing the impact of the program. Such a review would also be helpful to provide an insight on the methods that could be used in future for 1) remapping areas that are unsurveyed and not under MDA and areas bordering endemic districts; and 2) in identifying the appropriate sensitive tool that would detect ongoing transmission during post-MDA surveillance or validation phase. With this background, a systematic review of the literature on use of mapping and monitoring methods for LF elimination is undertaken.

Search criteria {#S0002}
===============

All published articles in national and international peer-reviewed journals were searched. Searches were made in PUBMED MEDLINE database with key words like "lymphatic filariasis" in combination with "monitoring," "mapping," "infection," "disease," "MDA," "antigeneamia," "antibody," "ICT," "FTS," "Wb123,\" "Mf" (or "microfilaria"), "vector infection," and "xenomonitoring" using Boolean operators like "and" and "or." Attempts were made to download the full articles and the back reference lists in these articles were searched for additional studies.

Eligibility criteria {#S0002-S2001}
--------------------

For a study to be eligible for the review it should either have carried out mapping or monitored/measured impact evaluation with respect to MDA or both in terms of filarial infection (Mf/Ag/Ab) in humans or disease in humans or infection in vectors.

Results {#S0003}
=======

The studies in this review were from the regions of Africa, Americas, Western Pacific, East Mediterranean and South-east Asia (as specified by WHO). The countries included from the African region were Benin, Botswana, Burkina Faso, Cameroon, Central African Republic, Côte d'Ivoire, Democratic Republic of the Congo, Ethiopia, Ghana, Malawi, Mozambique, Nigeria, Republic of the Congo, Rwanda, Senegal, Seychelles, Sierra Leone, Togo, Uganda, United Republic of Tanzania and Zambia; the studies in the American region were from Brazil and Haiti; the Western Pacific included studies from Papua New Guinea, Samoa, and American Samoa; Egypt and Sudan were the two countries from the East Mediterranean. The countries included from South East Asia were Bangladesh, India, Indonesia, Myanmar, Nepal, Sri Lanka, and Thailand. While in the African and East Mediterranean countries, where bancroftian filariasis is co-endemic with onchocerciasis (oncho), ivermectin plus albendazole were used for MDA, in the rest of the regions, where bancroftian filariasis is the only predominant form, DEC plus albendazole are being used.

Up until 2018, a total of 138 papers had been published on mapping, and or monitoring of LF. Of these, 54[@CIT0001],[@CIT0007]--[@CIT0059] were not eligible (as per the inclusion criteria) and therefore were excluded from subsequent review ([Figure 1](#F0001){ref-type="fig"}). A total of 84 studies were eligible for this review. Of these, 43 were on mapping alone, 36 on monitoring alone and five both on mapping and monitoring aspects of LF.Figure 1Selection of studies for the review.**Abbreviations:** MDA, Mass Drug Administration; STH, Soil transmitted helminths.

The results of this review on mapping and monitoring of LF are presented in accordance with different phases of the program (pre-MDA, during MDA and post-MDA). While one would expect mapping to be undertaken mostly prior to implementation of MDA, it was observed that mapping was also done in the during- and post-MDA phases. As for monitoring, the studies included in this review assessed only the impact of MDA and not on coverage or compliance aspects of MDA.

Mapping {#S0004}
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The majority (86%; 37/43) of the studies on mapping were conducted prior to implementation of MDA, mainly to delimit areas that required MDA. Of these 37 studies, 23 (62%) were from Africa, 5 (14%) from South East Asia, 6(16%) from the Americas and 3 (8%) from the Eastern Mediterranean region.

The WHO mapping guidelines[@CIT0005] for LF endemic areas (preferably an implementation unit (IU)/district) recommended a two stage sampling method of examining 50--100 individuals aged \>15 years (conveniently sampled) for filarial antigen from two communities purposively selected from the IU/district. If the Ag prevalence is found to be ≥1%, the IU/district is considered for MDA.

Pre-MDA {#S0004-S2001}
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The details of the pre-MDA mapping studies that were carried out to assess if MDA is required are given in [Table 1](#T0001){ref-type="table"}. The review showed that most African countries used the WHO recommended mapping method for delimiting areas requiring MDA.[@CIT0060]--[@CIT0068] The surveys were conducted among adults and or children aged 5--15 years across the studies spanning over countries. In addition to these surveys, a few studies used Mf surveys (current and historic data), key informant techniques[@CIT0069]-[@CIT0078] for assessing LF endemicity. In four studies, Mf, Ag and or disease surveys were used to map areas requiring MDA.[@CIT0079]--[@CIT0082]Table 1Details of the studies on mapping prior to MDAStudy no.WHO RegionCountryAuthor & yearStudy area (no. of communities)No. of districts requiring MDANo. examinedIndicatorAge (years)Findings**1**AfricaBenin, Burkina Faso, Ghana and TogoGyapong et al 2002[@CIT0060]401 rural or semi rural communities representing IU from these four countries.Not mentioned20,050 personsAg prevalence≥15Spatial analysis with RAGFIL data were tested in the four countries Benin, Bukina Faso, Ghana and Togo. The models could closely predict the Mf prevalences at all sampled points, with low or zero prevalence for Togo, Benin and eastern Ghana. This study provided the information that LF was more widely spread than it was thought to be earlier**2**South East AsiaNepalSherchand et al, 2003[@CIT0061]37 districts of 57 endemic districts33 out of 37 districts found endemic and require MDA4,488 personsMf, Ag and disease prevalence≥15Mapping was carried out in 37 districts in 2001 Results showed that 89% (33/37) of the districts were endemic and 11 had an Ag prevalence of more than 20%. The study also suggested the cross-benefits of different national intervention programs, particularly against TB and leprosy.**3**AmericasHaitiMadsen V.E Beau de rochars 2004[@CIT0062]Whole country (133 communes or districts)117 of 133 (87.9%) required MDA22,365 childrenAg prevalence6-11-The study was carried out to see the geographical distribution of LF in Haiti using the Ag survey among children aged 6--11 years among 113 communes of the country in 2001 (Rochars et al, 2004). The results when put across on maps indicated that LF was more widespread and suggested that the entire country will have to be considered for LF elimination programme.**4**AfricaMalawiNgwira et al, 2007[@CIT0063]35 villages of 23 districts29 villages required MDA2,913 personsAg prevalence≥15A national map was developed which incorporates data from surveys in Karonga, Chikwawa and Nsanje districts, carried out in 2000. There is a marked decline in prevalence with increasing altitude. Further analysis revealed a strong negative correlation (R^2^=0.7 *p*\<0.001) between altitude and prevalence. These results suggest that the lake shore, Phalombe plain and the lower Shire valley will be priority areas for the Malawi LF elimination programme. Implications of these findings as regards implementing a national LF elimination program in Malawi are discussed.**5**AfricaRwandaRuberanziza et al, 2009[@CIT0064]Five districtsNo MDA required797 personsAg prevalence\>15A rapid community-based mapping of LF was carried out in five districts of Rwanda by Ag surveys showed that only one was positive was LF and therefore it was declared that LF was not a public health problem in Rwanda**6**East MediterraneanSudanSturrock et al, 2009[@CIT0065]43 villages of Northern Bahr-el Ghazal StateAs per results, MDA is not requiredNot mentionedAg prevalenceAll agesIn a total of 43 villages in state, Ag surveys were carried out. This study used an integrated survey design for NTDs (STH, LF, schistosomiasis) to guide their control programme in the large area of Sudan. This approach was proved to be practical and simplified by reducing the survey work and costs. The exercise provided evidence that none of the areas needed MDA, confirming the cost-effectiveness of integrated mapping methods.**7**AfricaNigeriaIboh et al, 2012[@CIT0066]4 communities, Yakkurr Govt4 communities required MDA785 personsAg and disease prevalenceAll agesYakurr people of Cross River state of Nigeria living in four communities were tested for MF and LF disease. It was found that Mf and disease prevalence were 6.1 and 0.3%. These results were used to by the Government to expand the distribution of albendazole in the LF endemic region**8**AfricaZambiaShawa et al, 2013[@CIT0067]Luangwa districtPrevalence was 8.6%, require MDA546 personsMf,Ag and Ab prevalence≥1A pre-control epidemiological study for assessing the prevalence of infection (both Ag/MF), disease, and transmission and human perception aspects of LF. Results drug administration be initiated to accelerate this positive trend of decline in LF transmission in the area**9**AfricaZambiaMwase et al, 2014[@CIT0068]15 districts (14 endemic and 1 non-endemic)64 of 108 study sites required MDA9964 personsAg prevalenceAll agesThis study carried out Ag surveys, collected remote sensing data and did ecological niche modelling for filarial vectors distribution in the study area. Integrating all the above, it was shown that for areas with Ag prevalence ≥5% and those with ≥15%, land cover and land surface temperature respectively were significant predictors. The maps produced based on these indicated widespread occurrence of LF in Zambia, and the Ministry of Health in Zambia initiated mass drug administration iin late 2012, and scaled up this activity across the country in the next few years.**10**AfricaEthiopiaRebello et al, 2015[@CIT0069]658 districts with 1,315 communities75 of 658 districts with 89 communities require MDA1,30,166 personsAg prevalence≥15These are results of the integrated mapping of LF and Podoconiosis in seven regional states and two cities. Of the 658 districts surveyed, 75 were endemic for LF. Including the previous data on LF endemicity, a total of 112 districts require MDA**11**South East AsiaIndiaSabesan et al, 2000[@CIT0070]289 districts studied257 of 289 found endemic and require MDANot mentionedMf and disease prevalenceAll agesData from published studies on 289 districts was analyzed and showed that LF was endemic in 257 districts of 289 in India,**12**AfricaNigeriaAwolola et al, 2004[@CIT0071]Akinyele local government areaKey informant method95 KIsFilarial disease\>15Results suggest that the data from key informants on filarial disease cases is a rapid method to delimit areas with filariasis**13**South East AsiaIndiaChhotray et al, 2005[@CIT0072]24 villages from 2 districts of OrissaAll 110 villages found endemic and require MDA7304 personsMf and disease prevalenceAll agesThis was a baseline study in 24,110 villages that were surveyed for Ag and Mf in Puri/Ganjam districts and concluded that LF was widely distributed in the coastal districts of Orissa.**14**South East AsiaBiharDas et al 2006[@CIT0073]2 villages of Patna distirctMDA is required in 2 villages1872 personsMf and disease prevalenceAll agesResults showed that both villages were highly endemic with an Mf rate of 8.4% and a disease rate of 12%. Vector infection and infectivity rates were 14% and 8.2% respectively indicating on-going tranmsission in the study villages and that MDA is necessary.**15**South East AsiaIndiaSingh et al, 2006[@CIT0074]7 villages of Patna districtAll 7 villages found endemic and require MDA1878 personsMf prevalenceAll agesA baseline epidemiological study in rural areas of Patna district during pre-MDA period. A total of 7 villages were surveyed in from Patna district. Results showed that while Mf prevalnce was 6.2%, there was no infection in the vectors**16**AmericasBrazilBonfim et al, 2009a[@CIT0075]Municipality of Jaboatão dos Guararapes,Not mentioned23,673 personsMf prevalence≥1The Social deprivation index was useful in quantifying social inequalities thereby help in planning intervention. The strata constructed based on the index was helpful in indicating a risk gradient, with 74.9% of the microfilaremia cases situated in the high-risk stratum.**17**AmericasBrazilBonfim et al, 2009b[@CIT0076]Municipality of Jaboata ˜o dos Guararapes,Not mentioned23,673 personsMf prevalence≥1The socio-environmental composite risk index (SRI) was useful in mapping areas with higher risk of infection. As SRI could stratify spaces by using available official data, it is considered an important tool for use in the worldwide LF elimination program**18**AfricaCongoHope et al, 2011[@CIT0077]10 provinces and six citiesNot mentionedNot mentionedMf prevalenceAll agesAs the country is co-endemic for both loiasis and Onchocerciasis, a new mapping approach termed as Micro-stratification Overlap Mapping (MOM) is proposed prior planning MDA for LF . The authors reproduced the map with historical data of LF, overlapped with the maps of loiasis and onchocerciasis and provided key information about the ecology and transmission of *W. bancrofti*vectors in DRC. These maps were a useful resource for national LF programme in countries with co-endemicity as they provide information on areas with risk of serious adverse events and requires extra precautions or alternative intervention strategies**19**AmericasBrazilBrandao et al, 2011[@CIT0078]24 districts- 484 census tracts13 of 24 districts were hyperendemic and require MDA8670 childrenMf prevalence\<=18Mapping of filarial infection (Mf) and morbidity among children/adolescents was carried out prior to implementation of MDA. Results showed that transmission was intense among the pediatric population in 54% of the surveyed districts .Spatial analysis showed that the localities in which the populations most exposed to filarial transmission were concentrated.**20**AmericasBrazilBrandão et al, 2015[@CIT0079]Municipality of Jaboata ˜o dos Guararapes, State of PernambucoNot applicable8670 childrenMf prevalence\<=18The results on the survey on children and adolescents in combination with SRI (Bonfim et al, 2009) showed that the localities where the children most exposed to ﬁlarial transmission are concentrated. This index precisely measured the relationship between social deprivation and the prevalence of infection among children and cna be used in control and elimination activities.**21**AfricaSierra LeoneKoroma et al, 2012[@CIT0080]14 health districts14 districts require MDA1982 personsMf and Ag prevalence≥15Ag (ICT) survey was carried out in 14 health districts showed LF was endemic nationwide and that preventive chemotherapy was justified across the country. These data provided information for the NTDCP to design and implement MDA and the basis for future monitoring and evaluation of the national LF elimination programme.**22**AfricaEthiopiaShiferaw et al 2011[@CIT0081]125 villages of 112 districts34 of 112 districts require MDA11,685 personsAg and disease prevalenceAll agesThough the overall Ag prevalence was 3.7%, its distribution was found to be heterogeneous in these villages. Of these 112 districts 34 had prevalence rates more than 5% (Range: 4--20%) and these data were used to provide a tentative map of LF distribution in the study area.**23**AfricaNigeriaOkorie et al, 2013[@CIT0082]134 sitesNot applicable55,026 personsMf and Ag prevalenceAll agesThis review collated and mapped all LF data in Nigeria, to assess the extent of co-endemicity with loasis and determine the risk and benefits of different intervention strategies. It is suggested that integrating LF activities with that of STH and distributions of ITN/LLINs may have significant impact on both loasis and f LF.**24**AfricaCameroonDjeunga et al,2015[@CIT0083]120 districts106 of 120 (88.3%) require MDA26,586 personsMf and Ag prevalence≥5With a purpose of providing a quick and easy estimate on filarial endemicity status of the 90% of health districts in Cameroon and to obtain a country-wide map on LF, Ag survey was carried out in 120 health districts showed that 88% were eligible for MDA. (Ag prevalence:3.3% (95% CI: 3.0--3.7%),**25**AfricaMalawiNgwira et al, 2002[@CIT0084]12 villages, Shire valley, Songwe river12 study villags were require MDA685 adultsAg prevalence≥20Mapping of LF areas was carried out using Ag surveys in Karonga, Chikwawa and Nsanje districts. Results suggest that the lake shore, Phalombe plain and the lower Shire valley were found to be the priority areas for the Malawi LF elimination programme. The map so created showed that infection with W. bancrofti was more widespread than previously appreciated.**26**AfricaUgandaOnapa et al, 2005[@CIT0085]45 districts19 of 76 sites require MDA17,533 childrenAg prevalence5--19An Ag survey was carried out among school children (5--19 years) in 15 districts. The study suggested that screening of school children for Ag was a simpler and useful approach to mapping the geographical distribution of LF.**27**Eastern MediterraneanEgyptHassan et al, 1998[@CIT0086]Nile delta area covering 201 villages of 11 districtNot mentionedNot mentionedMf prevalenceAll agesThe first study on spatial analysis that looked into clustering of Mf prevalences (up to 2 km) at community level and showed heterogeneous pattern in filariasis transmission.Correlation between low Mf prevalence and higher humidity, low temperature and low rainfall were significant.**28**AfricaAfricaGyapong et al 2001[@CIT0087]87 communities selected using 25x25km grid sampling method; 30 communities of these were at 50x50km gridsNot mentionedNot mentionedAg prevalence\>15A spatial sampling grid of 50km interval between villages was used for sampling villages for rapid assessment of filariasis endemicity using Ag. This approach of mapping was recommended to capture the cross-border foci, which was found to exist. It was suggested that this method would provide the information for effective treatment planning.**29**AmericasBrazilMedeiros et al, 2012[@CIT0088]27 districts of Jaboatao dos Guararapes Municipality24 districts require MDA23,673 children/adolescentsMf prevalence1--18The data collected in an earlier study (Brandao et al, 2011), was used to identify the areas with high infection foci, applying kernel density approach on household level data. This method rapidly detected areas with highest concentration of infected cases and assisted the programme towards planning, monitoring, and surveillance of filariasis elimination activities. Infection in children/adolescents in combination with SRI showed that the localities where the children most exposed to ﬁlarial transmission were clustered and precisely measured the relationship between social deprivation and the prevalence of infection among children.**30**AfricaEthiopiaSime et al, 2014[@CIT0089]659 districts with 1,315 communities75 of 659 districts require MDA1,29,959 personsAg prevalence\>=15The two studies demonstrated that an integrated nationwide mapping of podoconiosis and LF in 659 districts with 1315 communities showed that this approach was feasible, cost effective and expanded geographical coverage and rapidly made available the data for decision makers.**31**East MediterraneanSudanFinn et al, 2012[@CIT0090]14 counties from 2 states11 out of 14 counties require MDA3,980 personsMf prevalence\>=16This one is the extension of the integrated mapping exercise (Sturrock et al, 2009) to three states of Sudan. In this study, three of the NTDs schistosomiasis, loasis and LF were mapped at county level. One to three sites with LF disease cases within the counties were sampled purposively and 250 individuals ≥16 years were examined for Ag(ICT). Results indicated only in two states the prevalence of Ag was \>2% and these were identified for MDA.**32**AfricaUgandaStensgaard et al, 2011[@CIT0091]Primary schools all over the countryNot mentioned17,533 childrenAg prevalence5-19A geo-statistical model was applied on the data collected in an earlier study (Onap et al, 2005) and predicted the LF and malaria prevalence in unsurveyed locations and determined the extent of geographical overlap of the two diseases. The model predicted areas with hyper-endemic *W. bancroft*i transmission and this was acclaimed to provide a better informed platform for integrated control by the health authorities of Uganda**33**AfricaAfricaSlater et al, 2012[@CIT0092]AfricaNot mentionedNot mentionedNot availableNot mentionedCombining correlative spatial modelling approaches with mechanistic models linking climate envrionmental /population to parasite transmission provide a useful solution of improving spatial predictions**34**AfricaAfricaSlater et al.2013[@CIT0093]AfricaNot mentionedNot mentionedNot availableNot mentionedA generalized linear spatial model fitted to the data on infection from published articles on Lf infection in Africa showed that the predicted LF prevalence to be highly heterogenous across Africa**35**AfricaSub-saharan AfricaMoraga et al, 2015[@CIT0094]Eastern, Middle, Northern and WesternNot applicableNot mentionedAg prevalenceAll agesMapping of LF was carried by predicting LF prevalence by integrated application of geostatistical and mathematical models. Mf data from 1,145 surveys conducted between 1950 and 2000 and Ag data from the Ag surveys conducted in from 1990 to 2000 were used for model building and predictions. The predictions showed that LF transmission is highly heterogeneous and the maps are expected to guide intervention, monitoring and surveillance strategies as countries progress towards LF elimination.**36**AfricaEthiopia, TanzaniaGass et al, 2017[@CIT0095]Ethiopia-45 districts,\
Tanzania-11 districtsThree of 45 districts in ethiopiarequire MDA40,868 childrenAg prevalence9--14A new tool for confirmatory mapping developed of LF and validated it against the mapping tool by WHO in 45 districts of Ethiopia and 10 MDA naïve districts of Tanzania. By avoiding unnecessary MDA in 52 districts, the confirmatory mapping strategy is estimated to have saved a total of \$9,293,219. Particularly in low prevalence setting, this new tool was shown to have the potential to save time, money, resources and avoid unnecessary treatments. With the 2020 elimination targets on the horizon, the confirmatory mapping tool may prove to be particularly useful for "shrinking the map" and conserving resources for use in areas where they are needed most.**37**AfricaEthiopiaSime et al, 2015[@CIT0096]45 districts3 districts require MDA18,254 personsAg prevalence9--14[^1]

In Rwanda, mapping showed that there was only one Ag positive case in all five districts that were surveyed and this helped in declaring that LF was not a public health problem and therefore MDA was deferred thereby saving money and other humane efforts.[@CIT0063]

In the countries of the African region, starting with rapid assessment procedures (Ag survey and key informant interviews on LF symptoms) for delimiting areas endemic for LF, the progress made on mapping of LF has been tremendous. A few studies mapped the distribution of LF[@CIT0061],[@CIT0062],[@CIT0066],[@CIT0083],[@CIT0084] using Mf and or Ag surveys and showed that the LF was more widely spread than it was previously thought in those countries. Few studies went further to carry out spatial analysis of the filarial prevalence data collected for mapping through a spatial sampling grid with 50 km distance between sampled locations and predicted LF prevalence at 25 km distance.[@CIT0060],[@CIT0077],[@CIT0085]--[@CIT0087]

Studies that carried out mapping of LF in Oncho co-endemic area helped the program to produce LF distribution maps[@CIT0080],[@CIT0082] with areas having Ag rates \>1% and recommended inclusion of albendazole with the monotherapy of ivermectin to aid elimination of LF in these areas.[@CIT0065] Subsequently, as the countries in the African region were known to be co-endemic for NTDs like loasis, soil transmitted helminthic diseases, schistosomiasis and podoconiosis, attempts were made to map all these under one roof and this paved the way for an integrated mapping approach to understand the extent of distribution of diseases, and implementing control programs. Integrated mapping of LF with podoconiosis led to efficient use of resources and helped in rapidly delimiting large geographical areas. This approach was popular among the health staff in Ethiopia.[@CIT0068],[@CIT0088] A large NTD survey integrating mapping for LF, schistosomiasis and STH in three states of Sudan[@CIT0089] established that an estimated population of 1.2 and 1.4 million individuals were eligible for regular MDA with preventive chemotherapy (PCT) to treat STH and schistosomiasis respectively, and that 1.3 million individuals residing in Central- and Eastern Equatoria required MDA for LF. This integrated mapping approach cut costs and saved time with the same man-power efforts.[@CIT0064] Yet another approach called Micro stratification overlapped mapping (MOM) in Congo[@CIT0076] helped the program authorities to take extra precautions in handling serious adverse events that may take place due to co-endemicity of LF and loasis. In Nigeria, a similar approach using historical data helped in producing a series of maps to assist in maximizing existing interventions, cost effective usage of resources as the LF elimination programme scaled up.[@CIT0081]

Countries such as Ghana, Uganda and India used the historical data (Mf, Ag), for predicting LF prevalence in the uncertain and un-surveyed areas using geo-statistical models[@CIT0067],[@CIT0069],[@CIT0090]--[@CIT0093] and showed the heterogeneous distribution of LF for the respective countries, indicating the possibility on the risk of occurrence of LF even in the probably non-endemic areas. Three studies from India used Mf prevalence to identify districts for MDA.[@CIT0071]--[@CIT0073] Mapping data along with social deprivation index (SDI) and socio-environmental composite risk index (SRI) were used in Haiti to specifically map LF risk in urban areas[@CIT0074],[@CIT0075],[@CIT0078] and to see clustering of infection. Two studies tested a confirmatory mapping tool against the WHO recommended mapping strategy mainly to assess whether those areas in Ethiopia and Tanzania[@CIT0094],[@CIT0095] with uncertain endemicity (one Ag positive among 200 adults; 45 in Ethiopia and 11 in Tanzania) needed to be included for MDA. This tool tested a sample of 320 children aged 9--14 years, from 30 schools selected based on probability proportional to estimated population size in a Woreda (a district) to find areas requiring MDA. The results showed that while only 3 out of 45 in Ethiopia and none of the 11 from Tanzania required MDA, thereby saving time and the resources by preventing MDA in areas where there was no evidence of transmission.

During MDA {#S0004-S2002}
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Mapping during MDA ([Table 2](#T0002){ref-type="table"}) was useful in re-assessing the geographic limits of LF and identifying new LF endemic areas that were either not surveyed, or classified as uncertain or non-endemic earlier.[@CIT0096]--[@CIT0098] It was observed that in India, 113 of the 190 districts, which were not surveyed earlier and not included for MDA, were identified to be having varying risk of transmission. As the elimination goal is nearing, the National Vector-Borne Disease Control Programme (NVBDCP) is in the process of validating these findings before bringing these districts under MDA net. Mf and entomological surveys conducted in the sentinel villages of the Gampha district of Sri Lanka after 5 rounds of MDA showed ongoing transmission[@CIT0099] and recommended continuation of further rounds of MDA. In another study in Malawi that had already undergone 5 rounds of MDA for LF elimination is co-endemic for oncho and malaria, it was felt that the intervention programmes against these diseases could have impacted the transmission of LF. Therefore, to identify the high-risk areas of LF, a multiple intervention score map (MISM), that is based on weighted sum of individual intervention scores was used. This score was useful in identifying areas with high and low coverage of LF impacting interventions. It was shown that those areas with low coverage with high baseline LF prevalence were identified and considered to be with high risk of transmission or re-emergence.[@CIT0100] Another study[@CIT0101] attempted morbidity mapping to estimate lymphedema burden in Malawi after 5 rounds of MDA, observed that as there was no standardized method for collection of morbidity data, the morbidity burden due to LF could be grossly underestimated.Table 2Details of the studies on mapping LF during and post-MDAStudy no.RegionCountryAuthor & yearStudy area (no. of communities)IndicatorAge (years)Findings**1**AfricaGhanaGyapong et al, 2001[@CIT0097]87 communitiesAg and hydrocele prevalence≥15The grid sampling methodology with Ag prevalence was a rapid method to assess the distribution of filariasis. Variogram analysis showed the presence of spatial dependency among the Mf prevalences of communities spaced at 25 km and 50 km grids. Prevalence contours predicted the infection prevalence in areas where data was not collected.**2**South East AsiaNepalDhimal et al, 2011[@CIT0098]Five administrative areasMXNot relevantThis study was carried out in endemic districts that had completed at least five rounds of MDA to define the geographical limits of the endemic zone of LF in high mountain areas. Results showed that LF cases were found in higher altitudes up to 1800 m asl and LF vectors at 2100 m asl. The study recommends MX to rule out any transmission at this altitude.**3**South East AsiaIndiaChand et al, 2016[@CIT0099]12 villages from four districts of Madhya PradeshMf and vector infection prevalenceAll agesIn India where MDA is currently on-going, Mf and mosquito surveys were carried out in the district considered non-endemic and not under MDA. Mf prevalence varied between 3.2 and 11.2%; vector infection rate: 2--13% and infectivity rate: 1.3--3.6%. The authors suggest accurate mapping of areas is essential to initiate MDA in the bordering areas of filarial endemic districts and areas as population migration may be high.**4**South East AsiaSri lankaWijegunawardana et al, 2012[@CIT0100]One district, Gampaha Mf and vector infection prevalence (MX)\>3 yearsThe study assessed the current status of LF after five rounds of MDA in two sentinel and one spot check site using GIS. While the Mf prevalence ranged between 0.5% and 3.4% in sentinel sites, vector infection by xenomonitoring ranged between 0 and 32.4%, indicating that transmission is on-going and therefore may need to intensify further rounds of MDA.**5**AfricaMalawiStanton et al, 2014[@CIT0101]Six LF-Oncho co-endemic districsAg prevalenceAll agesThis study quantified the geographical extent of LF (MDA), Oncho (MDA), Bednets and IRS interventions impacting LF and produced a multiple intervention score map(MISM--weighted sum of individual intervention score). Districts were classified into four groups based on baseline LF prevalence and MISM. High coverage areas included the LF-onchocerciasis endemic areas in the southern region of the country and areas along the shores of Lake Malawi, where malaria vector control had been prioritised. Three districts with high baseline LF prevalence measures but low coverage of multiple interventions were identified and considered to be most at risk of ongoing transmission or re-emergence. In this analysis, six districts were identified as priority districts for additional interventions, with a further five being identified as having low prevalence and high intervention coverage, suggesting that they should be prioritised for post-MDA surveillance and move to the elimination phase**6**AfricaMalawiSmith et al, 2014[@CIT0102]Chikwawa district hospital catchment areaChronic symptoms (lymphoedema)22--90Mapping of LF disease cases was done after five rounds of MDA showed that lymphoedema cases were nearer Shire river. Authors suggest that there is a need to develop new LF morbidity identiﬁcation and surveillance approaches to ensure that morbidity management strategies are effectively targeted.[^2]

Monitoring {#S0005}
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A total of 36 studies have monitored filarial infection during the period of MDA implementation and also during post-MDA surveillance. Of them, 19 were during MDA implementation, 15 during post-MDA, one study reported monitoring of both pre-MDA and during MDA, and another study about monitoring in all the three phases, (pre, during and post MDA) of elimination programme.

During MDA {#S0005-S2001}
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Of the 19 studies conducted during MDA phase, 6, 7, 3, 2 and 1 were from African, Southeast Asian, Western Pacific, Americas and eastern Mediterranean regions respectively ([Table 3](#T0003){ref-type="table"}). In eleven studies, the impact of MDA had been measured in terms of reduction in Mf Ag or Ab or filarial disease prevalence[@CIT0102]-[@CIT0112] in humans. Seven studies, two from India,[@CIT0113],[@CIT0114] two from Tanzania,[@CIT0115],[@CIT0116] one each from Haiti,[@CIT0117] Nigeria,[@CIT0118] Samoa,[@CIT0119] and Egypt[@CIT0120] monitored infection (xenomonitoring/dissection) in vectors in addition to infection in humans (MF/Ag/Ab).Table 3Details of the studies in which monitoring was done during MDAStudy no.RegionCountryAuthor & yearNo. of districts or villages or sitesNo. of people testedAge (years)IndicatorFindings**1**AmericasHaitiHochberg et al, 2006[@CIT0103]Five sites3,781 personsAll agesAg prevalenceIn this study, the researchers had explored the occurrence of systemic symptoms (fever, dizziness, headache, pruritus and myalgias) after the 5th round of MDA and its relation to filarial infection (Ag using ICT). Those who returned to the distribution posts seeking treatment of symptoms after the MDA were interviewed. Results showed that majority of the people reporting systemic and scrotal symptoms did not have detectable Ag levels at the time of reporting. The authors concluded that given the low doses of DEC and albendazole used in the MDA, it is less likely that the reported symptoms were caused by drug-related side effects. However, they queried whether treating such symptoms as a part of surveillance would affect MDA coverage and that it would vary from country to country.**2**South East AsiaIndiaMukhopadhyay et al, 2007[@CIT0104]Two urban areas and six rural villages of East godavari district5,056 personsAll agesMf, disease and vector infection prevalenceEast Godavari district in Andhra Pradesh, India had undergone six rounds of MDA from 1999 to 2005. Mf and entomological surveys were conducted in six villages and 2 towns of the district. The Mf and disease rates were 4.4% and 2.4% respectively. The vector infection and infectivity rates were 3.6 and 0,4% respectively. It stressed on the need to develop strategies to implement MDA more effectively by increasing the drug compliance.**3**South East AsianIndiaYuvaraj et al, 2008[@CIT0105]15 villages, Villupuram districtNot mentioned\>15Hydrocele prevalenceThis study was conducted during a long term community based trial to measure the effectiveness of MDA with DEC alone or Ivermectin alone where the coverage under MDA ranged between 54--75%. Cross-sectional clinical surveys were carried out before and after seven rounds of MDA. After seven rounds, hydrocele prevalence had declined from the pre-intervention level of 20.5--5.1% (*P*\<0.05) in the DEC arm, from 23.9% to 10.4% (*P*\<0.05) in the ivermectin arm and from 20.4% to 10.9% (*P*\<0.05) in the placebo arm, equivalent to reductions of 75.3%, 56.6% and 46.6%, respectively. However there was only a marginal decrease in lymphoedema in both arms. After the seventh MDA, there was a statistically significant decline in hydrocele prevalence in all other age groups in the communities treated with DEC. The impact was relatively less in ivermectin arm. Repeated DEC administration has the potential to prevent incidence of new hydrocele cases and may resolve the manifestation at least in a proportion of affected people.**4**Western PacificSamoaJoseph et al 2011[@CIT0106]Five villages2,474 personsAll ages & ≤10Mf, Ag and Ab prevalenceIn Samoa, inspite of 6 rounds of MDA, post treatment survey showed persistent Ag in one of the islands. Mf, Ag and Ab surveys (CELISA kit) were carried out to assess if transmission had interrupted in this island with persistent infection and in another island where transmission had interrupted. Results showed the need for strengthened control effects in these areas. The area, which was declared LF free was found to be endemic based on Ab survey in children. It concluded that more studies were required to validate the use of CELISA as a tool for assessing transmission.**5**Western PacificPapua New GuineaTisch et al, 2011[@CIT0107]Dreikikir district of East Sepik ProvinceNot mentioned≥5 yearsDisease prevalenceThis study measured the impact of an MDA trial (with single dose of DEC alone vs single dose of DEC plus ivermectin) after 4 rounds on acute filariasis morbidity(AFM) using a questionnaire. The annual incidence of AFM decreased by 20% during the year following the first MDA and by 49--61% in subsequent years relative to pre-MDA rates. AFM rates did not differ according to microfilaremic status or density (categorized as 0, 1--9, 10--99 and 100 microfilaria per ml) during any other study periods. Risk factor analysis showed that age, residence, Mf positivity, not taking MDA drugs were at greater risk of developing AFM. The authors concluded that the incidence of AFM in this population decreased to 57% of the pre-treatment level after 2 annual MDAs in areas of moderate transmission and by 61% in areas of high transmission irrespective of the drugs used for MDA. This rapid decrease in AFM incidence was sustained over the entire 5-year surveillance period, thereby highlighting the potential of MDA to alleviate this feature of LF morbidity.**6**South East AsiaIndiaShriram et al, 2014[@CIT0108]Five islands of Andaman and Nicobar islands2,561 personsAll agesMf prevalenceTransmission Assessment surveys (TAS) to decide on stopping or continuing MDA was carried out in a lone foci of infection, Nancowry group of Islands. It was found that inspite of 6 rounds of MDA, Mf prevalence was 3.3%. The authors had suggested mass distribution of DEC medicated salt as an adjunct to hasten elimination of infection in these islands.**7**South East AsiaIndonesiaDewi et al, 2015[@CIT0109]Three communities (one non endemic,\
one passed TAS,\
one failed TAS)1,543 children6--7Ab prevalenceThis study provided the epidemiological support for the use of Ab levels to determine "critical cut-off thresholds" in Brugia spp. areas. Results from districts which followed the current WHO guidance for mapping, MDA, and implementing TAS, while providing Ab profiles of treated and untreated populations under programmatic settings, supported the choice of Ab prevalence in the 6 and 7yo group in TAS for stopping MDA decisions.**8**AfricaEthiopiaEndeshaw et al, 2015[@CIT0110]Three villages774 persons≥2Ag prevalenceIt assessed the impact of 7 years of annual MDA with IVM monotherapy for oncho on LF and showed that it did not interrupt LF transmission. If LF was to be targeted along with oncho in the control program, albendazole should be added and treatment coverage should be improved.**9**South East AsiaBangladeshHafiz et al, 2015[@CIT0111]30 villages of Nilphamari district1,242 personsAll agesAcute disease prevalenceThe study assessed the impact of 12 rounds of MDA on clinical filariasis. A two-stage 30 cluster survey with selected villages were surveyed for Ag (ICT), episodes of ADLA and chronic LF manifestations. The study villages and the disease cases were mapped. Results showed there was no association between disease status and filarial Ag positivity. Twenty one of the 30 villages recorded at least one manifestation. Disease prevalence was 4.4 (3.4--5.7). The authors suggested the use of mobile apps for geo referencing the disease database at village level which may be helpful for scaling up MMDP activities and a home-based morbidity control protocol. However, there was no baseline information on disease in these areas to relate the change to the impact of MDA.**10**AfricaSenegalWilson et al, 2016[@CIT0112]14 villages in three districts1,131 persons≥5Ag and Ab prevalenceIt assessed the possibility of adding LF tests to a standard onchocerciasis epidemiological survey to provide meaningful results for LF-onco elimination program. The status of LF in three districts was assessed using ICT and Wb123, along with the Ov16 RDT in Kedougou region of Senegal, co-endemic for both oncho and LF. A convenience sample of individuals from 14 villages from three districts were examined for both *W. bancrofti*and *O. volvulus*infection. Prevalence of LF antigen was found as 0.5% (95%CI: 0.2--1.2%) and that of its antibody was 0.6% (0.2--1.3%). However, one of the three districts, Salemata recorded an Ag and Ab prevalence of 3.5% (1.3--7.4%) and 1.2% (0.2--4.2%). TAS required a sample of 1,500 children to assess interruption of LF transmission but in the current study the sample size was small and therefore it could not be determined if transmission was interrupted even if no ICT positives were detected. The findings of this study provided insight into the complexities that may arise if the stop MDA decision was to be taken in LF and Oncho co-endemic areas.**11**AfricaEthiopiaMengistu et al, 2017[@CIT0113]70 districtsNot mentionedNot mentionedMf and Ag prevalenceThe study described the current status of the LF elimination programme that was integrated with the onchocerciasis control program in 2009 that was implemented in 70 LF endemic districts. By 2016 it had achieved 100% geographical coverage with treatment coverages between 73 and 87% and an epidemiological coverage of 65%. Being a malarious region too, LLIN distribution and IRS also played a significant role in prevention of LF. The current status indicated that Ethiopia was poised to achieve the 2020 goal of elimination of LF if the treatment coverage was sustained and strong monitoring and evaluation were in place.**12**South East AsiaIndiaVaishnav et al, 2007[@CIT0114]One city (Surat)5,058 persons≥1Mf prevalenceThis study assessed the LF situation after 6 rounds of MDA in Surat ciy. LF endemicity rate reduced from 0.24% to 0.11% (reduction 54%) and in north zone it reduced from 0.72% to 0.30% (reduction of 58%). Though, overall Mf rate had reduced due to MDA, higher rate was noticed in North zone of city where the migrant populations influx was higher.**13**South East AsiaIndiaKhan et al, 2015[@CIT0115]Tea garden population of Dibrugarh, Assam634 personsAll agesMf, disease and vector infection prevalenceThe study assessed the current filariasis situation after five rounds of MDA among tea garden workers in Assam who had recorded high Mf rates in the past. Mf, disease and entomological surveys were conducted. While Mf rate was 3.8%, disease rate was 5.7%. Vector infection (13.2%) and infectivity (3.7%) rates were high. While the distribution coverage was \>80% in 2007, it reduced to 60--70% in 2013. However, there were no information on the compliance rates. The study concludes that LF elimination in the State is achievable but poor drug compliance was the main bottleneck to the elimination program. Community participation in adjunct to training of drug distributors for meeting the target of drug compliance were the essential components in the success of GPELF.**14**AfricaTanzaniaSimonsen et al, 2010[@CIT0116],[@CIT0117]One village (Kirare)919 persons≥1Mf, Ag and Ab prevalenceThese two studies from Tanzania, utilized the data from the surveys conducted in the Kirare village in Africa, which had completed four rounds of MDA. All individuals (only residents) in the village were examined under Mf, Ag(Og4C3) and Ab(Bm14) at the baseline and after each round of MDA upto 3rd MDA. Entomological surveys were also carried out for monitoring infection in vectors. However, children enrolled and assessed for Ag in standard Iimmediately after first MDA were followed upto 4th MDA and antigen levels were measured in them prior to each MDA. Results of these studies indicate that the prevalence of Ag and Ab post-4 MDA did not differ significantly from baseline levels. However, there were significant difference in Ag units and Ab OD values among the cohorts of individuals surveyed at both time points (Og4C3 Ag units: from 106.9 to 47.3 CFA units and Ab OD value: (from to 0.784 to 0.405). With regard to vector infection, the reduction was significant even after the first round of MDA. However the reduction in vector infectivity rates was not significant. Even the mean monthly transmission potential decreased by 87 times post 4th MDA. Ag prevalence in children of standard I immediately before the first MDA reduced significantly after 2 MDA onwards and reached 6.4% from 25.5%after the 4th MDA.**15**AfricaTanzaniaSimonsen et al, 201110 rural primary schools700--800 children6--14Ag prevalence**16**AmericasHaitiBoyd et al, 2010[@CIT0118]Six villages455 persons2-4Mf, Ag and Ab prevalenceThe authors described how even if the sentinel sites reported Mf prevalences \<1%, after eight rounds of MDA, transmission could still continue to occur through a 30-cluster survey among 2-4 yo. Ag and Ab prevalences were 14.3% and 19.7%. Filarial infection was focal in nature and infection was significantly associated with non-compliance to MDA drugs.**17**AfricaNigeriaRichards et al, 2011[@CIT0119]10 villages10,753 persons≥2Mf, Ag and vector infection prevalenceThe study assessed the impact of MDA (7 years of monotherapy with IVM in 12 LGAs) in a LF-Oncho co-endemic area. The villages were annually monitored till 2009, for both human and vector infection since 2000 and had completed 7--10 rounds of MDA with a coverage of 72% in 2003, ≥85% in 2006 and 73% by 2009. Impact on transmission showed that by 2009 Mf prevalence decreased from 4.9% to 0.8% (86% reduction), Ag (based on ICT) from 21.6% to 7.2% (67% reduction), vector infection from 3.1 to 0.4 (86% reduction) and infectivity rate from 1.3 to 0.3% (77% reduction). Three sentinel villages had on-going transmission (based on infective L3 in mosquito) and one had a Ag prevalence of 27.3% and vector infection \>2%, indicating the need for increased interventional efforts. The authors suggested that LGAs with greater endemicity (≥25% Ag prevalence at baseline) were likely to be the primary areas of risk of MDA failure. As the remaining LGAs were on the path of making decisions on stopping MDA, particularly for a co-endemic area there were two options: (i) IVM alone may have to be continued with post MDA surveillance for LF to prevent its recrudescence or (ii) after assessing the status of oncho transmission, if found interrupted stop Oncho MDA, and plan for an integrated surveillance method to carry out post-MDA surveillance for both the diseases.**18**Western PacificAmerican SamoaMladonicky et al, 2009[@CIT0120]Three villages579 persons≥5Ag and Vector infection prevalenceThe use of Ab and xeno monitoring in LF transmission was explored in an area that had completed 6 rounds of MDA. Mf, Ag and Ab surveys were conducted in three villages covering individuals aged \>4 years. The overall prevalence of Mf was \<1%, Ag prevalence ranged between 3.7 and 4.6% and Ab prevalence ranged between 12.5% and 14.9% in all these villages. As there were reports of vector infection in these villages (based on an earlier study), the authors concluded that both xeno-monitoring and Ab may be useful to identify areas with potential transmission of LF.**19**Eastern MediterraneanEgyptFarid et al, 2007[@CIT0121]Two sentinel sitesNot mentionedAll agesMf, Ag and Vector infection prevalenceThe study was conducted in Egypt, after five rounds of MDA to appraise if MX can be used as a tool for assessing progress towards elimination of LF. The tool was tested in two sentinel villages to measure the parasite DNA rates in mosquitoes. Results showed that the parasite DNA rates reduced by 93.8% and 100% in high and low prevalence areas respectively after five rounds of MDA. These changes were consistent with decreases in Mf prevalence rates in the sites. It also provided insight regarding the minimal mosquito DNA rates necessary for sustained transmission of filariasis in Egypt. The study concluded that MX is a powerful tool for assessing the impact of MDA.[^3]

Studies in the African,[@CIT0118] East Mediterranean,[@CIT0120] and South East Asian[@CIT0107],[@CIT0114],[@CIT0117] regions which used human and vector infection indicators have concluded that 4--6 rounds of MDA may not be sufficient to interrupt transmission. While the study from Nigeria[@CIT0118] concluded that additional interventions (like more frequent MDA treatments and insecticidal bed nets) were necessary particularly for those sentinel villages that had baseline Ag prevalence \>25%, systematic non-compliance to MDA was the reason for on-going transmission in Haiti which led to two additional MDA rounds.[@CIT0117] The studies that had used vector infection prevalence in addition to Mf/Ag/Ab[@CIT0117],[@CIT0119],[@CIT0120] have suggested that MX is a powerful tool in assessing the impact of MDA and that both Ab in humans and the MX tool could be used to measure on-going transmission.

Few studies that had measured Ab prevalence alone or in addition to Mf/Ag in children[@CIT0105],[@CIT0108],[@CIT0115],[@CIT0116] indicated that measuring Ab in children is a better indicator to assess interruption of transmission as it also measures exposure to infective bites in addition to infection with adult worms. It was suggested that measuring Ab instead of Ag alone may be helpful in identifying areas with residual infections.[@CIT0105]

Studies that used disease prevalence to measure the impact of MDA[@CIT0104],[@CIT0106],[@CIT0110] suggested that 10 rounds of MDA had helped in reducing the overall disease prevalence to low levels. A study from Papua New Guinea showed that after four rounds of MDA, the acute filarial morbidity reduced significantly from the pre-treatment levels and that the reduction was higher in those areas that had higher transmission indices compared to those with lower figures.[@CIT0106] In a community trial from India, it was found that seven rounds of MDA with DEC alone prevented incidence of new hydrocele cases and resolved the manifestation in a proportion of already affected individuals in addition to reducing the levels of Mf and transmission indices in the communities.[@CIT0104] Assessment of occurrence of systemic symptoms[@CIT0102] following a 5th round of MDA and providing free symptomatic treatment was expected to motivate people to report symptoms and seek care. However, the authors concluded that from a programmatic perspective, treatment of these systemic symptoms might not be necessary, because most of the individuals surveyed did not report these symptoms as a barrier to participating in future MDA.

Few studies have reported only the current situation of LF (Mf/Ag/Ab/disease) after six to seven rounds of MDA[@CIT0103],[@CIT0104],[@CIT0110],[@CIT0112]--[@CIT0114] in the endemic areas and found that Mf prevalence were still above the 1% threshold. To interrupt the on-going transmission in spite of several rounds of MDA, these studies recommend the need for additional efforts by program authorities to improve the coverage of the program and better monitoring and evaluation procedures.

One study assessed the possibility of integrating antibody testing for LF and oncho, which has highlighted the complexities that may arise in making a decision on stopping MDA particularly in LF-oncho co-endemic areas of Africa.[@CIT0111] Another study[@CIT0109] observed that 7 years of monotherapy with ivermectin has not reduced Mf prevalence in a oncho-LF co-endemic area and therefore to achieve significant reduction, the authors suggested that albendazole be added to the program particularly in LF endemic areas. In another oncho-LF co-endemic area[@CIT0118] the study showed that while Mf and Ag rates were reduced by 83% and 67%, the mosquito infection and infectivity rates were reduced by 86% and 76% compared to the baseline. As this was a LF-oncho co-endemic area, regarding the decision of stopping MDA, it was suggested that if LF transmission was found to be interrupted, albendazole could be stopped and the MDA could continue only with ivermectin with simultaneous post-MDA surveillance for LF for resurgence. If it is found that oncho transmission also has been interrupted, an integrated surveillance can be planned for post-MDA surveillance.

Post-MDA {#S0005-S2002}
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A total of 17 studies (eleven from South East Asia, three from Western Pacific, two from Africa and one from American regions) have reported the findings during the post-MDA monitoring phase. Of these, one additionally monitored pre-MDA period and another study monitored pre-, during- and post-MDA periods. These studies highlighted the importance of monitoring during different phases of MDA ([Table 4](#T0004){ref-type="table"}).Table 4Details  of the studies where monitoring was done post-MDAStudy no.RegionCountryAuthor & yearNo. of districts or villages or sitesNo. of people/mosquitoes testedAge (years)IndicatorFindings**1**AmericasHaitiChu et al, 2013[@CIT0122]11 countries29,169 children6--7Ag and Ab prevalenceThis study was carried out to assess the revised TAS protocol in its ability to make decisions to stop MDA and also to be used as a post-MDA surveillance tool. While Burkino Faso, Dominican republic, Ghana, Indonesia, Malaysia and Tanzania were in to make decision on stopping MDA, rest of the countries were already in the post_MDA scenario. The diagnostic tools used were ICT for *W.bancrofti* regions, and PanLF for TAS 1 or BMR1 for Brugia spp endemic areas. Sample sizes for these surveys were obtained through SSB (range: 684 in Sri Lanka to 1556 in Burkino Faso). Either school based or community based surveys were adopted. The authors suggest that TAS based on communities face several challenges than the ones based on school survey main reason being poor census, definition of EA boundaries, estimation of target age group etc., Preliminary results from separate TAS studies appear to suggest there is no statistically significant difference or change in the TAS-recommended outcome for EUs with school primary enrolment rates as low as 59% instead of the current 75% (Gass et al, 2013). The results of this study supports the reliability of this strategy but because TAS is not powered to detect change or designed to identify hotspots, post-MDA surveillance would best be complemented in the short and long term with other, complementary diagnostic tests and surveillance methods. In future, if new diagnostic tools are to be used, then the thresholds and the sample sizes may need to be modified.**2**South East AsiaIndiaSrivastava et al, 2014[@CIT0123]42 primary schools in Goa1692 children6--7Ag prevalenceThe study from Goa, describes the first time implementation of the revised TAS in India. Goa, one of the historically LF endemic districts in India, started the MDA against LF in the year 2000. Four rounds of MDA with DEC alone and 5 with DEC+albendazole were completed. Post-treatment MF survey showed that Mf rates in sentinel and spot check sites were 0. In 2013 Mf surveys in additional 10 randomly selected sites also showed 0 Mf prevalence. These observations showed that the district was eligible for TAS. A sample of 1692 (based on the SSB tool for TAS by WHO), children aged 6--7 years were tested for Ag (ICT) of which only 16 were positive, less than the cut-off value of 20, indicating passing of TAS. MDA was stopped and the district is now in the post_MDA surveillance mode. The authors conclude that TAS is a scientific, practical and effective evaluation tool for decisions on stopping or continuing MDA.**3**AfricaTanzaniaBiritwuma et al, 2017[@CIT0124]98 endemic districts of GhanaNot mentionedAll agesAb prevalenceThe study describes the existence of persistent LF hotspots inspite of 14 rounds of MDA . The study aimed to compare the baseline Mf prevalence and anti_filariasis interventions (LLINs) among hotspots and those districts where MDA was stopped. The study population were from the 29 hotspot districts and 69 stopped-MDA districts. The data collected were the baseline Mf prevalences and data on distribution of LLINs to the population. Assessing LF status at baseline, during MDA implementation and the status in 2016 showed that by 2014 69 districts had stopped MDA and by 2016 another 12 more stopped MDA after TAS. Results show that the number of rounds of MDA required for hotspots were higher than those districts where the MDA was stopped. It was also observed that the baseline MF prevalence in these hotspot districts were 10 time higher that those districts in which MDA has been stopped. The authors indicated that these observations may have implication on programme stating the districts with high baseline MF prevalence may require more than the recommended 5--6 rounds of MDA compared those with low baseline prevalence.**4**Western PacificAmerican SamoaShamsuzzaman et al, 2017[@CIT0125]34 districts (19 endemic and 15 low endemic)136,080 children6--7Ag prevalenceThis paper presents TAS results, highlighting the momentous geographical reduction in risk of LF and its contribution to the global elimination target of 2020. Since 2011, a total of 59 TAS have been conducted in 26 EUs across the 19 endemic MDA districts (99,148 students tested from 1,801 schools), and 22 TAS in the 15 low endemic non-MDA districts (36,932 students tested from 663 schools). All endemic MDA districts passed TAS, except in Rangpur which required two further rounds of MDA.The distribution was geographically sparse, with only two small focal areas showing potential evidence of persistent transmission. Bangladesh is now considered to have very low or no risk of LF infection after 15 years of programmatic activities, and is on track to meet elimination targets.Other positive influencing factors for Bangladesh include good administrative development and health system infrastructure, relative political stability, strong political commitment and financial support, strong program management leadership, heightened awareness of morbidity in the endemic areas, which helped to increase drug compliance and importance of disease elimination.**5**Western PacificPapua New GuineaKhieu et al, 2018[@CIT0126]Four sites (two sentinel and two spot check)Not mentioned≥5Ab prevalenceIn this study, as a first step towards LF elimination, mapping exercise was carried out in all provinces and districts, based on Ag surveys during 2000--2002. Any province with even one Ag positive as endemic. Two provinces and four districts in two other provinces classified as endemic due to the focal nature of LF. MDA with DEC and albendazole was initiated in 2005 through 2009 completing five rounds with effective coverage of 65%. In sentinel sites, Mf surveys were conducted during baseline and 2nd, 3rd and 4th years and also after the final round of MDA in 2009. In spot-check sites, mf surveys were carried out during 2nd, 3rd and 4th round of MDA. It was observed that Mf prevalence became 0% from third survey onwards and in sentinel sites it was 0% in all surveys. Hence TAS was conducted following the WHO norms and it was found that the Ag prevalence in six IUs (two provinces and four districts) ranged between 0.1 and 0.7%, much below the 1% level indicating interruption of transmission. MDA was stopped in these provinces and districts. Post MDA surveillance surveys TAS 2 on grade 1 children (after 2--3 eaof stopping MDA) and TAS 3 (after 46 years of stopping MDA) were conducted in 2013 and 2015 respectively. In both, there were no Ag positive child in both TAS2 and TAS3 surveys suggesting total transmission interruption in the IUs. To ensure this, TAS 3 with an antibody survey of children in one of the historically known LF endemic district was carried out and only one child was positive substantiating the earlier finding tha that transmission has been interrupted. Having passed TAS3, as the final stage of declaring elimination of LF, in 2015 the MoH prepared the dossier documenting elimination of LF as a public health problem and submitted to WHO which was validated by the Regional Dossier Review Group of WHO Western Pacific Regional office. In 2016, WHO headquarters officially acknowledged that LF elimination was achieved in Combodia.**6**South East AsiaNepalOjha et al, 2017[@CIT0127]Five districts9495 children6--9Ag prevalenceThis study in Nepal provides findings of the Pre-TAS, TAS and drug coverage surveys conducted after 6 rounds of MDA to assess if the LF transmission has been interrupted or not. The Study was conducted in 7 of the 10 endemic districts (selected purposively) prior implementing MDA. From each districts, 2 sentinel sites were selected (Based on migration, population size and LF prevalence). All sentinel and spot check sites in districts in five districts and one spot check site of another district reported \<2% Ag prevalence. Remaining 4 districts reported \>2% Ag in the pre-TAS surveys. None of these four districts at achieve epidemiological coverage of ≥65% MDA coverage. TAS was conducted in all these five districts and a part of the 6^th^ district (it was split into two EUs) and all passed TAS (number of Ag positive children less the cut-off value). The results suggest that the LF transmission was interrupted in the 5th and partly in 6th district and that MDA may be stopped in these. However in the remaining four, additional rounds of MDA may be necessary. out of the 10 district. Though the MDA coverage varied between the districts (50%-84%) they still passed TAS suggesting that a MDA coverage of around 50% may be sufficient to interrupt LF transmission in urban populations. However, in sptie of high coverage, four rural districts failed to pass TAS and these had the high baseline LF prevalence. These findings corroborate with the finding of Shamsuzzaman et al, 2017 described earlier.**7**South East AsiaMyanmarAye et al, 2018[@CIT0128]19 districtsIn 206 schools in 5 districts\>2Ag prevalenceThis study also, like the previous one summarizes the programmatic activities right from mapping endemic districts to post-MDA surveillance of LF, the progress and impact of the those activities and highlights the first evidence that prevalence has been lowered to a level when transmission is not sustainable. Data for this study was from 15 administrative units of Myanmar, consisting of 65 districts. Mapping exercise in 19 districts and historical data showed that 45 districts were endemic for LF. Starting MDA in only two districts in 2001, it was upscaled to all 45 endemic districts by 2014 so the number of MDAs completed ranged between 2 and 12 in different districts. Seven years after the start of MDA, the first TAS wasconducted in three districts and subsequently in other districts too. TAS passed in 5 districts and MDA was stopped, but monitoring continued in these IUs for next 5 years. The results of this study highlights that Myanmar NPELF has moved forward towards elimination of LF and significant reduction in Mf prevalence and with evidence for interruption of transmission.**8**South East AsiaIndiaSwaminathan et al, 2012[@CIT0129]Two Primary Health Centres35,582 personsAll agesAg prevalenceThis study was undertaken to develop sampling strategies to decide on stopping or continuing MDA in an implementation unit. Both Mf and Ag(Og4C3) prevalence were assessed by covering all individuals in the 92 villages to see the impact of 8 rounds of MDA (upto 2007). It was found that prevalence of Mf and Ag were 0.2% and 2.3%. In these 92 villages there were 7 residual (with Mf prevalence ≥1%) and 17 transmission (atleast one Ag-positive child born during MDA period) hotspots. It was also seen that inspite of eight rounds of MDA, there was spatial clustering of infection both at household and village level. The study highlights the need for identifying factors responsible for the emergence of ''transmission hotspots'' and adoption of appropriate sampling strategies for the development of evidence-based programmatic decision-making tools.**9**South East AsiaIndiaRamaiah et al, 2007[@CIT0130]Ten communites7% of Households, adults and 20--40 children≥15 & 1--10Mf prevalenceTen rounds of mass drug administration was done in 10 communities (5 each for DEC alone and Ivermectin alone) and 49---84% of the eligible population received treatment in different villages. Out of five villages in each treatment arm, the mf rate declined to ≤1% in four villages in the DEC arm and two villages in the ivermectin arm. No mosquitoes with infectivestage larvae were found in three of five villages in the DEC arm and two of five villages in the ivermectin arm. None of the children (n=130) were found to be positive for mf in either treatment arm. None of the 40 sampled children were found to be positive for circulating filarial antigenaemia in villages with lower endemicity in the DEC arm. The results suggest that ten rounds of DEC mass administration have the potential to interrupt transmission of infection in the majority of communities. The outcome was relatively less remarkable with ivermectin.**10**South East AsiaIndiaRamaiah et al, 2013[@CIT0131]Five villages700 persons and 10842 mosquitoes\>2Mf, Ag and Vector infection prevalenceRobust monitoring and evaluation of MDA is necessary to assess its impact and to stop MDA when the indicators of impact -- Mf prevalence in the population or vector infection rate or antigenemia (Ag) prevalence in the children born during the MDA period -- fall below the threshold level. The impact of 10 rounds of MDA (using DEC alone) on LF infection and transmission in 5 endemic communities of south India, were monitored and evaluated for 6 years after the overall Mf rate of the study communities was brought down to,1.0%, considered to be the safe and threshold level to stop MDA. Overall Mf prevalence (n=700) and vector infection rates (n=803--3520) showed a declining trend. Both Mf status in humans and infection in vectors were zero from 3rd year after stopping MDA. In only one village, community Mf rate was at 1.0% and Ag prevalence among 1--7 -year old children was 4.6% (n = 44) and vector infectivity rate during the sixth year was 0.1% (n = 852).**11**South East AsiaIndiaRamaiah et al, 2002[@CIT0132]10 villages588 personsindividuals with ≥15 kg weightMf prevalenceThis placebo-controlled study examined the potential of six rounds of mass treatment with DEC or IVM to eliminate *Wuchereria bancrofti*infection in humans in rural areas in south India. The results indicate that DEC is as effective as or slightly better than IVM against microfilaraemia. Results from this and other recent operational studies proved that single-dose treatment with antifilarials is very effective at community level, feasible, logistically easier and cheap and hence a highly appropriate strategy to control or eliminate LF. Higher treatment coverage than that observed in this study and a few more than six cycles of treatment and more effective treatment tools/strategies may be necessary to reduce microfilaraemia to zero level in all communities, which may lead to elimination of LF.**12**South East AsiaIndiaSubramanian et al, 2015[@CIT0133]33 villages/wards in Tanjavur district20,049 mosquitoesNot relevantParasite DNA rateThe monitoring and evaluation of lymphatic filariasis (LF) has largely relied on the detection of antigenemia and antibodies in human populations. Molecular xenomonitoring (MX), the detection of parasite DNA/RNA in mosquitoes, may be an effective complementary method, particularly for detecting signals in low-level prevalence areas where Culex is the primary mosquito vector. This article investigated the application of a household-based sampling method for MX in Tamil Nadu, India. Households were systematically selected using a sampling interval proportional to the number of households in the EU. Mosquito pools were collected and analyzed by real-time polymerase chain reaction (qPCR).The household-based sampling strategy for MX led to mostly reproducible results and supported the observed LF infection trends found in humans. MX has the potential to be a costeffective, non-invasive monitoring and evaluation tool with sensitive detection of infection signals in low prevalence settings**13**Western PacificAmerican SamoaLau et al, 2016[@CIT0134]32 villages of American Samoa376 personsAll agesAg and Vector infection prevalence (MX)The study evaluated xeno-monitoring as a surveillance tool by linking village level results of published human and vector studies. A total of 32 villages were included in the study. While a 34.4% were positive for Ag (by Og4C3), 56.3% positive for Wb123 Ab and 84.4% were positive for Bm14 Ab. Parasite DNA in vectors was detected in 15 of the 32 villages. Particularly in those villages which recorded Ag positive and Wb 123 positive persons, parasite DNA was found in 91% and 72% of the vectors. In those villages that had no positive persons (for Ag or Wb123), PCR positivity for vector infection were absent.**14**South East AsiaBangladeshIrish et al, 2018[@CIT0135]30 villages in two districts10,021 mosquitoesNot relevantParasite DNA rateIn this study, MX evaluation was conducted in two areas of Bangladesh, one previously endemic district that had stopped MDA (Panchagarh), and part of a non-endemic district (Gaibandha) that borders the district where transmission was most recently recorded.The results showed that none of the mosquito pools tested were positive for*W. bancrofti*DNA which confirms the results of TAS conducted during 2013 and 2015. The authors suggest that MX can be used to identify missing foci of transmission with smaller geographical areas, in areas were Ag positive cases were identified in TAS**15**South East AsiaSri LankaRao et al, 2014[@CIT0136]30 health administrative units28,000 mosquitoesNot relevantParasite DNA rateGalle district (population 1.1 million) was divided into two EUs. These included a coastal EU with known persistent LF and an inland EU with little persistent LF. Mosquitoes were systematically sampled from \~300 trap locations in 30 randomly selected clusters (health administrative units) per EU. Approximately 28,000 *Culex quinquefasciatus*were collected with gravid traps and tested for filarial DNA by qPCR. 92/625 pools (14.7%) from the coastal EU and 8/583 pools (1.4%) from the inland EU were positive for filarial DNA. Maximum likelihood estimates (MLE) for filarial DNA rates were essentially the same when the same number of mosquito pools were collected and tested from 75, 150, or 300 trap sites (range 0.61--0.78% for the coastal EU and 0.04--0.07% for the inland EU). The ability to use a smaller number of trap sites reduces the cost and time required for mosquito sampling. These results suggest there is widespread persistence of *W. bancrofti*infection in the coastal Galle EU 8 years after the last round of MDA in 2006, and this is consistent with other data from the district. This study has shown that MX can be used by national programs to assess and map the persistence of *W. bancrofti* at the level of large EUs in areas with Culex transmission.**16**AfricaTanzaniaRebollo et al, 2015[@CIT0137]Two sentinel sites (islands of Unguja and Pemba)3,275 children6--7Ab prevalenceThe study from Tanzania was conducted in Zanzibar where five rounds of MDA with IVM and albendazole was completed and resulted in zero prevalence of MF, Ag and vector infection in sentinel and spot check villages. MDA was stopped and TAS was performed to assess if transmission was interrupted. The study was conducted in islands Pemba and Unguja of Zanzibar. Two sentinel sites from the islands of Unguja were used for monitoring and data on Mf prevalence and coverages were collected at the baseline (prior to MDA) and repeated before each round of MDA. The Mf rates in these two sites reduced from 17.8% and 17.2% to 1.0% and 0.0% respectively. Following this, TAS was conducted in the two islands and children were examined for Ag (ICT). However the number of Ag positive children was much higher than the cut-off value in Pemba but within the cut-off value for Unguja. The low prevalence of Ag in children in Unguja is attributed vector control measures like usage of LLINs and IRS. However, earlier entomological studies in Unguja immediately after stopping MDA showed that transmission was on-going in Unguja islands. The authors indicate that 5 years of MDA may not be sufficient for interrupting LF transmission. Based on this study, the government of Zanzibar decided to resume MDA with ivermectin and albendazole in both these islands in 2013.TAS will be conducted in 2015.**17**South East AsiaSri LankaRao et al, 2016[@CIT0138]19 PHIs17,479 children,\
78,000 mosquitoesAll ages for MF and Ag, Ab in 6--7Mf, Ag, Ab and Vector infection prevalenceComprehensive surveillance was performed in 19 Public Health Inspector (PHI) areas (subdistrict health administrative units) 6 years after stopping MDA. The surveillance package included cross-sectional community surveys for microfilaremia (Mf) and circulating filarial antigenemia (CFA), school surveys for CFA and anti-filarial antibodies, and collection of Culex mosquitoes with gravid traps for detection of filarial DNA (molecular xenomonitoring, MX). Provisional target rates for interruption of LF transmission were community CFA,2%, antibody in school children,2%, and filarial DNA in mosquitoes,0.25%. Community Mf and CFA prevalence rates ranged from 0--0.9% and 0--3.4%, respectively. Infection rates were significantly higher in males and lower in people who denied prior treatment. Antibody rates in school children exceeded 2% in ten study sites; the area that had the highest community and school CFA rates also had the highest school antibody rate (6.9%). Filarial DNA rates in mosquitoes exceeded 0.25% in 10 PHI areas.[^4]

As seen in pre- and during-MDA phases, studies in the post-MDA phase have also used Ag/Ab and vector infection indicators to monitor the LF situation. In a multi-country study (consisting of eleven countries) Chu et al.[@CIT0121] reported that TAS protocol is a reliable strategy (either school- or community-based) to assess interruption of transmission. Similar observation was made in India[@CIT0122] who reported the first successful TAS in Goa in India after nine rounds of MDA, mentioning that TAS is a scientific, practical, and effective evaluation tool for stopping MDA. The authors emphasized the importance of focal survey to identify the source of infection in communities with antigen-positive children to prevent resurgence in an area declared free from MDA.

A study from Ghana reported the existence of hotspots, despite 14 years of MDA, and attributed the persistence of hotspots to its high baseline Mf prevalence[@CIT0123] and reported that more than the standard five to six rounds of MDAs, say up to 14 rounds were required to interrupt transmission. Another study[@CIT0124] summarized the current situation of LF in Bangladesh through TAS conducted in 19 districts (which had undergone 5--12 rounds of MDA) and 15 low endemic districts (not given MDA). The results showed a significant reduction in prevalence of geographical distribution of infection across Bangladesh and attributed this to factors like good administrative development and health system infrastructure, relative political stability, strong political commitment and financial support, strong program management leadership and heightened awareness of morbidity in the endemic areas. The authors also suggested that districts that required more rounds of MDA had higher baseline Mf prevalence. Post-MDA surveillance in Cambodia[@CIT0125] showed that all the endemic districts had undergone five rounds of MDA with \>65% coverage and had passed even TAS 3 and is currently in the final stage of declaring LF elimination. The authors have attributed this success to the commitment of the government and effective implementation of MDA, monitoring and evaluation, and surveillance activities. MDA was successfully implemented even in forested and remote endemic areas through advocacy, sensitization of various departments, active participation of provincial- and central-level program personnel in MDA activities, and financial and related support from partners and stakeholders. In Nepal[@CIT0126] it was shown that interruption of transmission was established in five of the ten endemic districts and in one EU of another district following successful TAS after six rounds of MDA, in-spite of not achieving the optimum coverage of 65% in three districts. In the remaining districts, MDA continued as they had a higher baseline Mf prevalence as reported in Bangladesh.[@CIT0124]

In Myanmar[@CIT0127] the program initiated in one district in 2001 was up-scaled to 42 of the 45 endemic districts by 2014. This slow progress in upscaling was attributed to lack of funding, resources (eg training of basic health staff, advocacy materials), continued problems in procuring adequate quantities of DEC, as well as security-related issues. In spite of this, five districts declared interruption of transmission with two passing TAS 1 after 6--10 rounds of MDA and three districts that had passed TAS 2 after undergoing five rounds. In this study also, a predictive modeling approach showed that the decline in Mf prevalence was associated with the number of MDAs and initial Mf prevalence as observed in the above studies.

The PHC area in Thanjavur district, Tamil Nadu, India,[@CIT0128] reported low levels of infection (community Mf and Ag prev-0.2%,and 2.3%, and Ag prevalence in 2--8 years old children was 0.12%) after eight rounds of MDA with spatially clustered infection both at household and community level. Based on this study MDA was stopped in this area. Results of a long-term community trial that evaluated the effectiveness of ten rounds of DEC or ivermectin alone in ten villages of the Villupuram district of Tamilnadu, India[@CIT0129]-[@CIT0131] concluded that ten rounds of MDA with DEC alone would be able to interrupt transmission of LF and achieve the objective of LF elimination.[@CIT0129] Subsequent post-MDA study[@CIT0130] in the same area indicated that 6 years of post-MDA monitoring and evaluation was adequate to discern the status of transmission interruption and for making an appropriate decision on certifying LF elimination.

Few studies have also evaluated the use of MX for confirming the findings of TAS and, also as a post-MDA surveillance tool. In a study from India, Ag-prevalence in children and vector infection indicators were used to assess the LF situation in one of the evaluation areas that stopped MDA (after 8 rounds).[@CIT0128],[@CIT0132] The estimated vector infection rate based on xenomonitoring corroborated with the decision based on human infection for stopping MDA in this evaluation unit, suggesting that xenomonitoring could be a potential alternative to post-MDA surveillance or validation. Further, vector infection prevalence was reported to be higher in communities which were transmission hotspots (with Ag positive children). Similar observations were made in American Samoa[@CIT0133] that detected parasite DNA in vector mosquitoes in the communities where Ag-positive persons were detected. In Bangladesh, one study[@CIT0134] reported MX evaluation for transmission interruption in two areas; one previously endemic district that had stopped MDA (Panchagarh), and another part of a non-endemic district that reported transmission. In a study from Sri Lanka, in addition to TAS, a comprehensive surveillance (a package included cross-sectional community surveys for Mf and Ag, school surveys for Ag and Ab, besides xenomonitoring) was carried out to assess the LF situation 6 years after cessation of MDA in 19 Public Health Inspector (PHI) areas of the eleven EUs in eight districts.[@CIT0135] The prevalence of infection in vector exceeded the provisional threshold of 0.25% in 10 of the 19 PHIs where the school- and community-based Ag- and Ab surveys reported \<2% Ag and \>2% Ab prevalence. Based on the results, the authors recommended use of antibody and MX testing as tools to complement TAS for post-MDA surveillance. Similarly, a study from Tanzania showed that in areas that had passed TAS, MX provided evidence of on-going transmission and this led to re-initiation of MDA for another two rounds in those areas. In a subsequent study from Sri Lanka,[@CIT0136] MX indicated ongoing transmission (filarial DNA prevalence of 0.63% greater than the provisional threshold of 0.25%[@CIT0135]) in a coastal district 8 years post-MDA, although Mf prevalence (0.2%) was much lower than the threshold for stopping MDA (1%) proving xenomonitoring is more sensitive than TAS and Mf-survey.

Mapping and monitoring {#S0006}
======================

Five studies reported on mapping and monitoring together ([Table 5](#T0005){ref-type="table"}) during MDA (four studies) and post-MDA phase (one study).Table 5Details of the studies that carried out both mapping and monitoringStudy no.RegionCountryAuthor& yearNo. of districts or villages or sitesIndicatorNo examinedAge (years)Findings**1**South East AsiaHaitiDrexler et al, 2012[@CIT0139]Five communesAg prevalence2639 children4-17In the study from Haiti (Drexler et al, 2017), the authors have explored if there is active transmission particularly in communities that were identified as low prevalence five communes (during mapping exercise in 2001-- Ag prevalence in 6--11-year-olds). A follow-up school surveys for Ag in children were carried out in 2003. For community survey, to examined atleast 100 individuals per community, 20 households within a radius of 5075 m (in urban, peri-urban area) and 100--250 m (rural) of 5--8 index cases (Ag positive children detected in school survey) were selected. Households were selected by systematic sampling method. All individuals available in the selected household was tested for Ag(ICT) and another 200 ul of blood was collected for confirmatory test (using Og4C3). Results of the school survey showed that 2.8% of the 4--17-year-olds were Ag positive. While ICT tested positive children in all five communities. Og4C3 detected Ag positives only in three of the five communities. Analysis on distance from index cases showed that the relationship between Ag positivity and distance from index case was strongest at 20 m and it became weaker with decreasing Ag prevalence with increase in distance.**2**South East AsiaIndiaUpadhyayula et al, 2012[@CIT0140]120 villages from four districts (Karimnagar, Chittoor, East and West Godavari)Mf and vector infection prevalence23,624 personsAll agesThe study was conducted in the state of Andhra Pradesh in India (Upadhyayula et al, 2012). This study is primarily on spatial mapping and analysis of LF over a 3-year period from 2004 to 2007, during which the MDA was implemented.Blood samples were collected from individuals and data on entomology and socio-economical aspects were collected. Mf prevalence in these districts was 31.5% (with the least being 8.9%) and the disease rate ranged between 0.5% and 2.3%. All the villages were geo-referenced with way points, and these were used to create maps on filariasis at village level. While both east and west Godavari districts were hyper endemic, Karim Nagar and Chittoor fell under medium endemicity. The spatial mapping showed various levels of infection even after implementation of MDA**3**AfricaTanzaniaSimonsen et al, 2014[@CIT0141]Seven districts of Tanga regionMf and Ag prevalence2753 persons for Ag\
555 persons for Mf\
1700 mosquitoesAll agesThe study describes the LF control started in rural areas of Tanga region of Tanzania in 2004 completing 8 rounds currently. Levels of transmission and human infection decreased initially, and became less pronounced in subsequent years. Even after 6 MDAs transmission was on-going at a reduced level. Seven other districts in the Tanga region (total 8 districts) were included for monitoring in 2013. Spot check community and school based were conducted in Tanga district. Four hamlets of Kirare village were for the community study and standard I pupils (6--7-year-olds) were examined for Ag (ICT) every year. In other districts, two villages were purposively selected from each district. A total of 200 6--7-year-olds were screened for Ag during 2013. Those positive for Ag only were bled for Mf smears. Vector surveillance was done using light traps in 50 selected households of Kirare village. Mapping was done by using the geo-coordinates of the sites and kriging was used to estimate prevalence in regions that were not surveyed. Results from Tanga district surveys indicate that both Mf and Ag prevalences reduced compared to baseline levels. Prevalence of chronic filarial manifestations (hydrocele/elephantiasis) was less than half of that in 2004. Dissection of msoquitoes post MDA 7 and eight rounds showed no filarial infections. Ag prevalence in 6-7yo since the start of MDA had a declining trend and after the 8th round, the prevalence was 2.3% (91% reduction compared to pre-MDA level of 25%). The authors conclude that though there is a positive downward trend in LF transmission and human infection, LF is still widespread in many parts of the Tanga region even after eight rounds of MDA, particularly in coastal areas. Lower infection rates in inland districts suggests that MDA could be stepped down after rigorous assessment so that resources could be diverted to upscale control activities in coastal districts.**4**Western PacificAmerican SamoaLau et al, 2014[@CIT0142]Three islandsAg and Ab prevalence555 personsAll agesIn this study (Lau et al, 2014), the success story of MDA for 5 years (2000--2006) following which the MDA was stopped (based on TAS) and the post MDA surveillance thereafter is described in detail. The LF antigen (Og4C3) and antibody (Wb123 and Bm14) tests were performed on the adult serum samples (n=807) from a serum bank where the samples were collected for a study on leptospirosis, 4 years after the last effective round of MDA. Information on the residence of the participant whose serum was used was used to look into geographical clustering of serologically positive cases. Results showed that which Ag prevalence was 0.75%, antibody prevalences were 8.1% for Wb123 an 17.9% for Bm14. Antigen and antibody prevalences were inversely associated with number of years of living in American Samoa. While antigen prevalence showed spatial variation, antibody prevalence did not show any spatial pattern. Higher prevalence of antigen in adults shows that residual infection is still there in the communities. Further, migrants (from neighbour LF endemic areas) showed higher antigen prevalences. Authors suggest that TAS may not be able to detect transmission hotspots. In the discussion the authors suggest that the risk of LF and drivers of transmission are likely to be heterogeneous within any evaluation units, and could be influenced by many factors such as climatic conditions, population density, urban versus rural areas, MDA coverage, and vector species and density. Therefore the average prevalence in an evaluation unit could mask focal areas of high prevalence (hotspots) if they are surrounded by large areas of low prevalence. Hotspots are more likely to be missed if they are small, in evaluation areas with greater spatial heterogeneity, and when prevalence is very low such as in the post-MDA surveillance phase.**5**Western PacificPapua New GuineaGraves et al, 2013[@CIT0143]324 survey sites between 1980 and 2011 in 80 districtsMf and Ag prevalence37,425 persons for MF\
43,264 persons for ICT\
16,221 persons for Og4c3All agesThis study is a review in which the LF elimination programme in Papua New Guinea at district level since 1980 to 2011, ie pre and during MDA. Data from a total of 312 district level surveys were utilized for this study. Data on Mf and Ag(ICT and Og4C3) were collected. Results indicate that, on combining these data, the estimates for Mf, ICT and Og4C3 when crudely averaged over districtswere 18.5% for Mf, 10.1% for ICT and 45.5% for Og4C3. Comparison in terms of diagnostic methods showed that Mf prevalence was always lower than ICT prevalence in most surveys. Only one survey reported higher Mf prevalence than that of Ag prevalence (using Og4C3). With respect to time period over every 10 years, it was seen that there was a decline in Mf and Og4C3 prevalences. However no obvious decline was observed in Ag prevalence based on ICT. Results indicated that there was large variation in LF prevalence in the country which may be related to the diverse altitude, geographical and ecological factors and also to the mosquito vectors in different regions of the country. Further, the heterogeneity could be due to the previous malaria control activities and recent distribution of insecticide mosquito nets and to some extent the MDA for LF in limited sites. It was also shown that the number of LF endemic districts differed with GPELF criteria specified earlier and the modified forms. While the earlier criteria classified 60 endemic districts, the modified gave only 36 endemic districts. The alternative criteria defining endemic districts as that one with ≥1% Ag prevalence classified only 34 as endemic districts. On the whole, based on these criteria, about 20 districts do not require MDA as per GPELF criteria 1 and 2. At the outset this review highlights the gaps in data and the knowledge on the remaining unknown districts.**Abbreviations:** LF, Lymphatic filariasis; DEC, Diethylcarbamazine; IVM, Ivermectin; Ag, Antigeneamia; Mf, Microfilaraemia; Ab, Antibody; MDA, Mass drug administration; TAS, Transmission Assessment Survey; ICT, Immunochromatographic Test; MX, Molecular Xenomontoring.

In Haiti, of the five districts that were mapped as low endemic based on Ag survey prior to MDA,[@CIT0137] transmission was on-going during MDA in three districts and a possibility of spatial dependency up to a distance of 20 m from an index case (an Ag-positive child). Spatial mapping of Mf prevalence (overall 31.5%, least 8.5%) after three rounds of MDA in four endemic districts of Andhra Pradesh in India showed spatial heterogeneity with non-interruption of transmission.[@CIT0138]

A study in Tanzania[@CIT0139] showed that even after six rounds of MDA, transmission was on-going but at a lower level. However, the Ag prevalence in 6--7-year-olds had started showing a declining trend. One study,[@CIT0140] using samples from a serum bank assessed the prevalence of LF Ag and Ab, 4 years after stopping MDA. They reported that while Ag prevalence was very low (0.8%), Ab prevalence (8.1%) was high as expected. It was seen that while Ag prevalence showed spatial variation, Ab prevalence did not. Another study[@CIT0141] from Papua New Guinea that reviewed the district level distribution of MDA during 1980--2011, reported that targeting of districts by level of prevalence will strengthen the control program, facilitate monitoring of the disease trend and increase the likelihood of reaching the target of LF elimination by 2020.

Discussion {#S0007}
==========

This review on mapping and monitoring of LF is based on 84 studies from 32 countries representing all WHO regions except the European region over two decades between 1997 and 2018. Most African countries followed the mapping protocol for delimitation in addition to historical data. Countries from other regions used WHO mapping strategy and other methods like the Key informant technique,[@CIT0070] physical examination by health workers for chronic symptoms of filariasis, disease prevalence and Mf prevalence for mapping LF. The review has shown how the WHO mapping strategy that initially used convenience sampling design evolved to become a more robust 30-cluster-based design in line with the confirmatory mapping tool[@CIT0094],[@CIT0095] to delimit areas requiring MDA. This confirmatory mapping tool tested Ag among older children aged 9--14 years in 30 randomly selected schools and was proved to be more effective in low-prevalence settings. Though this tool is similar to the LF-TAS, the sample size requirement is only one-fourth of that of TAS and covers older children and is currently termed as "Mini-TAS." In the current scenario, where most LF endemic countries have completed at least five rounds of MDA, it is proposed to use this for re-mapping in uncertain and naive areas bordering the endemic districts or the unsurveyed areas that have the risk of LF transmission to help the country program to bring in or exclude those areas from the MDA net. However, the challenge will be to choose the appropriate test (Ag/Ab surveys in humans or MX for vector infection) for re-mapping to identify new transmission hotspots if any, and prevent resurgence during post-MDA surveillance.

This review has highlighted how mapping helped in implementing the elimination program only in the areas known to be endemic for LF thereby preventing MDA in areas where the LF was non-endemic. Mf and Ag surveys were used in prioritizing areas requiring MDA and these data were subsequently used to visualize the extent of LF distribution. Accounting for the spatial component in these data with appropriate spatial models, LF prevalence was predicted up to a distance of 25--50 km around the surveyed locations to provide additional information on risk of LF to the program authorities to act upon.[@CIT0060],[@CIT0090],[@CIT0093],[@CIT0096]--[@CIT0098] Such data also provided scientific evidence on the clustering nature of LF around a transmission/residual foci which again could be used to extend MDA or other supplementary interventions in those neighboring areas. In addition, mapping data collected over time from different countries were also useful for modeling the trends of LF prevalence accounting for the effect of climate change on parasite transmission.[@CIT0091],[@CIT0092]

The mapping initiative for LF also paved the way to integrate mapping of all NTDs (LF, podoconiosis, schistosomiasis, loasis and STH) which was proven to be cost effective and feasible while covering a large geographical area.[@CIT0064],[@CIT0088],[@CIT0089],[@CIT0100],[@CIT0142] However, it could be logistically intensive and methodologically difficult due to the difference in the tests used and the target groups to be covered. As this is one of the important activities under the WHO road map[@CIT0143] for implementing interventions against NTDs, the challenges for the endemic countries would be in terms of strong in-country leadership, man power and resources to conduct the surveys and application to capture the data to make it cost effective and feasible.

One of the reasons for the success of this large scale elimination program is attributed to the strong in-built component of monitoring and evaluation during- and post-MDA. The review has shown that impact of MDA is generally measured in terms of Mf and Ag prevalence in humans. However, in recent times Ab prevalence in humans and MX for assessing parasite DNA in vectors are being used for the purpose in view of their high sensitivity in detecting exposure/transmission. It was observed that the WHO recommended strategy of 5--6 rounds of MDA was not sufficient in interrupting transmission[@CIT0103],[@CIT0107],[@CIT0109],[@CIT0111],[@CIT0114],[@CIT0118],[@CIT0119],[@CIT0123] suggesting the requirement of additional interventions like frequent MDA treatments, distribution of insecticide treated nets and other localized vector control measures[@CIT0118] Additional efforts warranting improved MDA coverage and adequate monitoring by the implementing authorities were recommended. Non-interruption of transmission and persistence of hotspots in spite of several rounds of MDA (up to 12 rounds) were found to be significantly associated with high baseline prevalence of Mf or Ag[@CIT0118],[@CIT0123],[@CIT0124] and systematic non-compliance to MDA.[@CIT0117] These imply that the number of rounds required to reach the threshold of \<1% Mf prevalence (or 2% Ag prevalence) cannot be generalized for all endemic areas. The success stories in Bangladesh[@CIT0124] and Cambodia[@CIT0125] indicated that in addition to the adequate MDA coverage, the reasons for success of the elimination program in a country is driven by 1) the commitment of the government and effective implementation of MDA even in remote areas; 2i) active participation of provincial- and central-level program personnel; 3) adequate financial and related support from stakeholders and partners; and 4) a rigorous monitoring and evaluation plan and post-MDA surveillance activities. The impact of MDA on LF disease, both chronic and acute forms were promising with not only reduction in the disease prevalence following 5--8 rounds of MDA but also in preventing new cases of hydrocele, thereby indirectly achieving the MMDP goals too.[@CIT0104],[@CIT0106]

In LF-oncho co-endemic areas, impact assessment surveys during MDA showed that several rounds of MDA with monotherapy of Ivermectin alone, ^100,\ 107,\ 113^ could not interrupt LF transmission and inclusion of albendazole in the subsequent rounds helped in reducing the Mf and Ag prevalence in humans and vector infection and infectivity rates significantly, respectively.[@CIT0109],[@CIT0111] However, the decision on stopping MDA in such areas would imply stopping albendazole but continuing with ivermectin alone along with post-MDA surveillance to ensure there is no resurgence of the disease.

TAS, the decision-making tool for stopping or continuing MDA was adjudged to be a robust, scientific and reliable tool based on the results from 12 countries,[@CIT0121] including India,[@CIT0122] based on a single EU. Detection of filaria parasite DNA in vectors in areas with Ag-positive children, showed that the results of TAS and MX corroborate with each other, when the decision to continue MDA is to be made. However in a few countries, it was also shown that assessing the impact of MDA on transmission interruption was more accurate if Ag tests in TAS are complemented with Ab tests during post-MDA surveillance as they provide additional evidence of on-going transmission/exposure in the community, particularly in children.[@CIT0105],[@CIT0115],[@CIT0116],[@CIT0132],[@CIT0134] In some instances, even when both Ag (TAS) and Ab tests indicated interruption of transmission, vector infection prevalence through MX was found to be well above the provisional threshold level of 0.5% (indicating on-going transmission) suggesting that TAS may not be a sensitive tool in assessing complete interruption of transmission,[@CIT0133],[@CIT0135],[@CIT0136],[@CIT0142] particularly during post-MDA surveillance. In view of this, it was recommended MX/Ab surveys may be used as a complementary tool for TAS to assess transmission, particularly during post-MDA surveillance and validation phases.[@CIT0133],[@CIT0135],[@CIT0136],[@CIT0142] These observations suggest that though TAS is a powerful tool while making decisions on stopping MDA or not, it may or may not be very sensitive particularly during the post-MDA surveillance and validation phases when the transmission levels are very low. Therefore MX, a sensitive tool in detecting on-going transmission may also be done alongside so that there is added support to the results by TAS. However, implementing MX under programmatic mode will be a major challenge as the expertise and the facilities for running the molecular assays need to be made available at all implementation unit levels.

Currently, in order to eliminate LF in hard core areas with persisting transmission, WHO has recommended the use of triple drug regimen (IDA, Ivermectin + Diethylcarbamazine + Albendazole) for MDA towards accelerated elimination of LF by 2020.[@CIT0006] While the monitoring and impact evaluation methods are in place for the existing LF elimination strategy of MDA with two drug regimens (DA, DEC + Albendazole),[@CIT0004] the challenge will be to develop an appropriate monitoring and evaluation (M & E) strategy, as this strategy is expected to interrupt transmission with 2--3 rounds of MDA in the endemic areas. In addition to the appropriate indicators sensitive enough to measure the human infection levels and or vector infection/infectivity levels, the target population need to be examined and the diagnostic to be used in humans for assessing transmission interruption needs to be identified.

Conclusions {#S0008}
===========

The review has highlighted the necessity of mapping for LF before a massive intervention program is to be implemented in large geographical area as it helps in identifying only those areas that require the intervention, thereby saving resources and man-power. If the area is endemic for other NTDs as well, an integrated mapping approach would be cost effective as well as feasible. For example in India, if an area is prone for dengue, chikungunya and LF, serological or MX surveys with appropriate test kits/devices with good sampling designs can be used to carry out integrated mapping of these diseases at the same time sharing the man-power and other resources.[@CIT0144] However, to include this as a part of a national program, a substantial investment has to be made in providing the infrastructure and training to personnel to carry out serological and MX surveys for the NTDs prevalent in the area. The review compared the performance of both serological (Ag/Ab) and molecular tools in assessing the status of LF transmission and highlighted that MX could play a major role in the context of M & E for LF. As most endemic countries under GPELF are progressing towards post-MDA surveillance, validation and elimination phases, M & E component needs to be revised/strengthened with appropriate tools either Ag complemented with Ab or MX for verifying the absence of transmission, assessing the risk of resurgence, identifying hotspots, particularly in the context of introduction of IDA as MDA for accelerated elimination.

With this holistic approach, national programs should take advantage of the existing serological tools and MX and incorporate them wherever necessary in their integrated vector management program to control, monitor and eliminate all vector borne diseases. And wherever possible both mapping of diseases and monitoring of interventions should adopt an integrated approach, to assess the current situation with minimum resources.

Disclosure {#S0009}
==========

The authors report no conflicts of interest in this work.

[^1]: **Abbreviations:** LF, Lymphatic filariasis; DEC, Diethylcarbamazine; IVM, Ivermectin; Ag, Antigeneamia; Mf, Microfilaraemia; Ab, Antibody; MDA, Mass drug administration; ICT, Immunochromatographic Test; EU, Evaluation unit.

[^2]: **Abbreviations:** LF, Lymphatic filariasis; DEC, Diethylcarbamazine; IVM, Ivermectin; Ag, Antigeneamia; Mf, Microfilaraemia; Ab, Antibody; MDA, Mass drug administration; ICT, Immunochromatographic Test; MX, Molecular Xenomontoring.

[^3]: **Abbreviations:** LF, Lymphatic filariasis; DEC, Diethylcarbamazine; IVM, Ivermectin; Ag, Antigeneamia; Mf, Microfilaraemia; Ab, Antibody; MDA, Mass drug administration; TAS, Transmission Assessment Survey; ICT, Immunochromatographic Test; MX, Molecular Xenomontoring.

[^4]: **Abbreviations:** LF, Lymphatic filariasis; DEC, Diethylcarbamazine; IVM, Ivermectin; Ag - Antigeneamia; Mf, Microfilaraemia; Ab, Antibody; MDA, Mass drug administration; TAS, Transmission Assessment Survey; ICT, Immunochromatographic Test; MX, Molecular Xenomontoring.
